AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46314.t1 MTR_7g096150 94.853 136 7 0 1 136 1 136 7.29e-83 239
MS.gene46314.t1 MTR_1g070495 73.984 123 31 1 15 136 15 137 3.49e-52 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46314.t1 AT2G30570 57.664 137 53 3 1 136 1 133 2.27e-43 139

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATGAGGATGAAGATTC+TGG 0.217541 7.3:+15205616 MS.gene46314:CDS
TGGATCTTGTTTGGTGTCTT+TGG 0.223893 7.3:+15205556 MS.gene46314:CDS
TTGGTGTCTTTGGTCTTATA+TGG 0.276875 7.3:+15205566 MS.gene46314:CDS
CTTCTTGGTTGGATCTTGTT+TGG 0.292250 7.3:+15205547 MS.gene46314:CDS
GGACTTAGCAACAATCTTCT+TGG 0.299425 7.3:+15205532 MS.gene46314:CDS
CTTCCAACCTTAGCTATTGA+TGG 0.334379 7.3:-15205343 None:intergenic
GACCTGCTTCCTTGCACATC+AGG 0.343789 7.3:-15205391 None:intergenic
GAAGGAACTGGCCTTCCCTT+TGG 0.386325 7.3:+15205511 MS.gene46314:CDS
TCTACCAAAGCCATGGCTGC+TGG 0.411412 7.3:-15205472 None:intergenic
TGTTGAGGTGCCAGCAATAA+TGG 0.413871 7.3:-15205157 None:intergenic
AAAACTTACCAACAATGGTC+TGG 0.428853 7.3:-15205233 None:intergenic
AAAATGGCAACCATTATTGC+TGG 0.451832 7.3:+15205147 None:intergenic
AAGCCCAGCAGCCATGGCTT+TGG 0.453307 7.3:+15205468 MS.gene46314:CDS
TTAGCAACAATCTTCTTGGT+TGG 0.463955 7.3:+15205536 MS.gene46314:CDS
TTTGTGCACAAGCAACCTCT+TGG 0.465311 7.3:+15205201 MS.gene46314:CDS
AGAATGAGCACTGAAGGAAC+TGG 0.491605 7.3:+15205499 MS.gene46314:CDS
CTACCAAAGCCATGGCTGCT+GGG 0.492560 7.3:-15205471 None:intergenic
AGCAGAGATAGCTGCTATCA+AGG 0.498626 7.3:-15205434 None:intergenic
TTGATGAGGATGAAGATTCT+GGG 0.502222 7.3:+15205617 MS.gene46314:CDS
AGCAGGTCAAACATAGGAAT+GGG 0.505396 7.3:+15205406 MS.gene46314:CDS
CAGAATCTTCATCCTCATCA+AGG 0.515447 7.3:-15205615 None:intergenic
GTCCCATCCCAGACCATTGT+TGG 0.518566 7.3:+15205225 MS.gene46314:CDS
AACCTGATGTGCAAGGAAGC+AGG 0.522854 7.3:+15205389 MS.gene46314:CDS
CTTGTCAAGCCCAGCAGCCA+TGG 0.525662 7.3:+15205462 MS.gene46314:CDS
AGAGTGAGATGTTCCATTGA+GGG 0.532339 7.3:+15205364 MS.gene46314:CDS
AAACTTACCAACAATGGTCT+GGG 0.543127 7.3:-15205232 None:intergenic
AAGCAGGTCAAACATAGGAA+TGG 0.548495 7.3:+15205405 MS.gene46314:CDS
AAGATTGTTGCTAAGTCCAA+AGG 0.554462 7.3:-15205527 None:intergenic
CTACGCTTCCTCCCTTGATG+AGG 0.562437 7.3:+15205603 MS.gene46314:CDS
TACCAACAATGGTCTGGGAT+GGG 0.563076 7.3:-15205227 None:intergenic
TTGCCATCAATAGCTAAGGT+TGG 0.567060 7.3:+15205340 MS.gene46314:CDS
AGATTGTTGCTAAGTCCAAA+GGG 0.569327 7.3:-15205526 None:intergenic
TTACCAACAATGGTCTGGGA+TGG 0.569922 7.3:-15205228 None:intergenic
ACATCTTCAATCATCCGCAC+AGG 0.569929 7.3:+15205177 MS.gene46314:CDS
GATGAGAGAATGAGCACTGA+AGG 0.576075 7.3:+15205493 MS.gene46314:CDS
CATGGCTGCTGGGCTTGACA+AGG 0.577276 7.3:-15205461 None:intergenic
AGGATTGCCATCAATAGCTA+AGG 0.584181 7.3:+15205336 MS.gene46314:intron
AAGAGTGAGATGTTCCATTG+AGG 0.585764 7.3:+15205363 MS.gene46314:CDS
CAAGGAAGCAGGTCAAACAT+AGG 0.586835 7.3:+15205400 MS.gene46314:CDS
AGAATCTTCATCCTCATCAA+GGG 0.614208 7.3:-15205614 None:intergenic
GAGGGAAAACCTGATGTGCA+AGG 0.626356 7.3:+15205382 MS.gene46314:CDS
TCTCTCATCTACCAAAGCCA+TGG 0.628797 7.3:-15205479 None:intergenic
ATCTTCATCCTCATCAAGGG+AGG 0.635880 7.3:-15205611 None:intergenic
TGTTGCTAAGTCCAAAGGGA+AGG 0.637762 7.3:-15205522 None:intergenic
GCGGATGATTGAAGATGTTG+AGG 0.660911 7.3:-15205172 None:intergenic
GTCTGGGATGGGACTCCAAG+AGG 0.664580 7.3:-15205216 None:intergenic
GCTTGTGCACAAAACCTGTG+CGG 0.820156 7.3:-15205191 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTCAAACATAAATTGTAT+AGG + chr7.3:15205316-15205335 MS.gene46314:intron 20.0%
! TTTGATGAAGATGAAATTGT+TGG - chr7.3:15205273-15205292 None:intergenic 25.0%
TAGGAAAAACTTACCAACAA+TGG - chr7.3:15205241-15205260 None:intergenic 30.0%
! AAATTGTTGGTTGATTCACT+AGG - chr7.3:15205260-15205279 None:intergenic 30.0%
AAAACTTACCAACAATGGTC+TGG - chr7.3:15205236-15205255 None:intergenic 35.0%
AAACTTACCAACAATGGTCT+GGG - chr7.3:15205235-15205254 None:intergenic 35.0%
AAGATTGTTGCTAAGTCCAA+AGG - chr7.3:15205530-15205549 None:intergenic 35.0%
AGAATCTTCATCCTCATCAA+GGG - chr7.3:15205617-15205636 None:intergenic 35.0%
AGATTGTTGCTAAGTCCAAA+GGG - chr7.3:15205529-15205548 None:intergenic 35.0%
TTAGCAACAATCTTCTTGGT+TGG + chr7.3:15205536-15205555 MS.gene46314:CDS 35.0%
TTGATGAGGATGAAGATTCT+GGG + chr7.3:15205617-15205636 MS.gene46314:CDS 35.0%
!! TTGGTGTCTTTGGTCTTATA+TGG + chr7.3:15205566-15205585 MS.gene46314:CDS 35.0%
AAGAGTGAGATGTTCCATTG+AGG + chr7.3:15205363-15205382 MS.gene46314:CDS 40.0%
AAGCAGGTCAAACATAGGAA+TGG + chr7.3:15205405-15205424 MS.gene46314:CDS 40.0%
AGAGTGAGATGTTCCATTGA+GGG + chr7.3:15205364-15205383 MS.gene46314:CDS 40.0%
AGCAGGTCAAACATAGGAAT+GGG + chr7.3:15205406-15205425 MS.gene46314:CDS 40.0%
AGGATTGCCATCAATAGCTA+AGG + chr7.3:15205336-15205355 MS.gene46314:intron 40.0%
CAGAATCTTCATCCTCATCA+AGG - chr7.3:15205618-15205637 None:intergenic 40.0%
CTTCCAACCTTAGCTATTGA+TGG - chr7.3:15205346-15205365 None:intergenic 40.0%
CTTCTTGGTTGGATCTTGTT+TGG + chr7.3:15205547-15205566 MS.gene46314:CDS 40.0%
CTTGATGAGGATGAAGATTC+TGG + chr7.3:15205616-15205635 MS.gene46314:CDS 40.0%
GGACTTAGCAACAATCTTCT+TGG + chr7.3:15205532-15205551 MS.gene46314:CDS 40.0%
TTGCCATCAATAGCTAAGGT+TGG + chr7.3:15205340-15205359 MS.gene46314:CDS 40.0%
!! TGGATCTTGTTTGGTGTCTT+TGG + chr7.3:15205556-15205575 MS.gene46314:CDS 40.0%
ACATCTTCAATCATCCGCAC+AGG + chr7.3:15205177-15205196 MS.gene46314:CDS 45.0%
AGCAGAGATAGCTGCTATCA+AGG - chr7.3:15205437-15205456 None:intergenic 45.0%
ATCTTCATCCTCATCAAGGG+AGG - chr7.3:15205614-15205633 None:intergenic 45.0%
CAAGGAAGCAGGTCAAACAT+AGG + chr7.3:15205400-15205419 MS.gene46314:CDS 45.0%
GCGGATGATTGAAGATGTTG+AGG - chr7.3:15205175-15205194 None:intergenic 45.0%
TCTCTCATCTACCAAAGCCA+TGG - chr7.3:15205482-15205501 None:intergenic 45.0%
TGTTGCTAAGTCCAAAGGGA+AGG - chr7.3:15205525-15205544 None:intergenic 45.0%
TTACCAACAATGGTCTGGGA+TGG - chr7.3:15205231-15205250 None:intergenic 45.0%
TTTGTGCACAAGCAACCTCT+TGG + chr7.3:15205201-15205220 MS.gene46314:CDS 45.0%
! CACATCAGGTTTTCCCTCAA+TGG - chr7.3:15205380-15205399 None:intergenic 45.0%
! TACCAACAATGGTCTGGGAT+GGG - chr7.3:15205230-15205249 None:intergenic 45.0%
! TGTTGAGGTGCCAGCAATAA+TGG - chr7.3:15205160-15205179 None:intergenic 45.0%
!! AGAATGAGCACTGAAGGAAC+TGG + chr7.3:15205499-15205518 MS.gene46314:CDS 45.0%
!! GATGAGAGAATGAGCACTGA+AGG + chr7.3:15205493-15205512 MS.gene46314:CDS 45.0%
AACCTGATGTGCAAGGAAGC+AGG + chr7.3:15205389-15205408 MS.gene46314:CDS 50.0%
GAGGGAAAACCTGATGTGCA+AGG + chr7.3:15205382-15205401 MS.gene46314:CDS 50.0%
GCTTGTGCACAAAACCTGTG+CGG - chr7.3:15205194-15205213 None:intergenic 50.0%
CTACCAAAGCCATGGCTGCT+GGG - chr7.3:15205474-15205493 None:intergenic 55.0%
CTACGCTTCCTCCCTTGATG+AGG + chr7.3:15205603-15205622 MS.gene46314:CDS 55.0%
GACCTGCTTCCTTGCACATC+AGG - chr7.3:15205394-15205413 None:intergenic 55.0%
GTCCCATCCCAGACCATTGT+TGG + chr7.3:15205225-15205244 MS.gene46314:CDS 55.0%
TCTACCAAAGCCATGGCTGC+TGG - chr7.3:15205475-15205494 None:intergenic 55.0%
!! GAAGGAACTGGCCTTCCCTT+TGG + chr7.3:15205511-15205530 MS.gene46314:CDS 55.0%
AAGCCCAGCAGCCATGGCTT+TGG + chr7.3:15205468-15205487 MS.gene46314:CDS 60.0%
CATGGCTGCTGGGCTTGACA+AGG - chr7.3:15205464-15205483 None:intergenic 60.0%
CTTGTCAAGCCCAGCAGCCA+TGG + chr7.3:15205462-15205481 MS.gene46314:CDS 60.0%
! GTCTGGGATGGGACTCCAAG+AGG - chr7.3:15205219-15205238 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 15205150 15205651 15205150 ID=MS.gene46314
chr7.3 mRNA 15205150 15205651 15205150 ID=MS.gene46314.t1;Parent=MS.gene46314
chr7.3 exon 15205150 15205246 15205150 ID=MS.gene46314.t1.exon1;Parent=MS.gene46314.t1
chr7.3 CDS 15205150 15205246 15205150 ID=cds.MS.gene46314.t1;Parent=MS.gene46314.t1
chr7.3 exon 15205338 15205651 15205338 ID=MS.gene46314.t1.exon2;Parent=MS.gene46314.t1
chr7.3 CDS 15205338 15205651 15205338 ID=cds.MS.gene46314.t1;Parent=MS.gene46314.t1
Gene Sequence

>MS.gene46314

ATGGCAACCATTATTGCTGGCACCTCAACATCTTCAATCATCCGCACAGGTTTTGTGCACAAGCAACCTCTTGGAGTCCCATCCCAGACCATTGTTGGTAAGTTTTTCCTAGTGAATCAACCAACAATTTCATCTTCATCAAATAAAATGACATAGCAATTCATTTCTTTCAAACATAAATTGTATAGGATTGCCATCAATAGCTAAGGTTGGAAGAGTGAGATGTTCCATTGAGGGAAAACCTGATGTGCAAGGAAGCAGGTCAAACATAGGAATGGGAGCATCCTTGATAGCAGCTATCTCTGCTGCAACCTTGTCAAGCCCAGCAGCCATGGCTTTGGTAGATGAGAGAATGAGCACTGAAGGAACTGGCCTTCCCTTTGGACTTAGCAACAATCTTCTTGGTTGGATCTTGTTTGGTGTCTTTGGTCTTATATGGACTCTCTATTTTATCTACGCTTCCTCCCTTGATGAGGATGAAGATTCTGGGTTGTCTCTATAA

Protein sequence

>MS.gene46314.t1

MATIIAGTSTSSIIRTGFVHKQPLGVPSQTIVGLPSIAKVGRVRCSIEGKPDVQGSRSNIGMGASLIAAISAATLSSPAAMALVDERMSTEGTGLPFGLSNNLLGWILFGVFGLIWTLYFIYASSLDEDEDSGLSL