AlfalfaGEDB | MS.gene63538

M. sativa cultivar XinJiangDaYe / MS.gene63538

KEGG: K08905
GO: GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009522 GO:0009987 GO:0015979 GO:0016020 GO:0032991 GO:0044237 GO:0098796 GO:0110165
PFAM: PF01241.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63538.t1	AFK46340.1	99.4	162	1	0	1	162	1	162	8.60E-83	316.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63538.t1	Q9S7N7	70.3	165	41	3	1	162	1	160	4.5e-56	218.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63538.t1	I3T1E8	99.4	162	1	0	1	162	1	162	6.2e-83	316.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049346	MS.gene63538	0.812795	3.54E-51	-1.69E-46
MS.gene049356	MS.gene63538	0.816267	6.04E-52	-1.69E-46
MS.gene049369	MS.gene63538	0.807613	4.64E-50	-1.69E-46
MS.gene049427	MS.gene63538	0.843701	1.19E-58	-1.69E-46
MS.gene049491	MS.gene63538	0.810209	1.29E-50	-1.69E-46
MS.gene049609	MS.gene63538	0.846053	2.76E-59	-1.69E-46
MS.gene049614	MS.gene63538	0.808323	3.28E-50	-1.69E-46
MS.gene049661	MS.gene63538	0.882694	8.40E-71	-1.69E-46
MS.gene049662	MS.gene63538	0.886396	3.55E-72	-1.69E-46
MS.gene049805	MS.gene63538	0.811923	5.49E-51	-1.69E-46
MS.gene050076	MS.gene63538	0.832744	7.94E-56	-1.69E-46
MS.gene050301	MS.gene63538	0.849712	2.71E-60	-1.69E-46
MS.gene050397	MS.gene63538	0.849493	3.12E-60	-1.69E-46
MS.gene050404	MS.gene63538	0.838001	3.73E-57	-1.69E-46
MS.gene050406	MS.gene63538	0.845332	4.34E-59	-1.69E-46
MS.gene050432	MS.gene63538	0.872954	2.14E-67	-1.69E-46
MS.gene050529	MS.gene63538	0.846156	2.59E-59	-1.69E-46
MS.gene050538	MS.gene63538	0.803311	3.70E-49	-1.69E-46
MS.gene050593	MS.gene63538	0.825028	5.86E-54	-1.69E-46
MS.gene050670	MS.gene63538	0.866495	2.73E-65	-1.69E-46
MS.gene050747	MS.gene63538	0.805958	1.04E-49	-1.69E-46
MS.gene050767	MS.gene63538	0.853605	2.14E-61	-1.69E-46
MS.gene050770	MS.gene63538	0.855898	4.63E-62	-1.69E-46
MS.gene050772	MS.gene63538	0.851767	7.16E-61	-1.69E-46
MS.gene050783	MS.gene63538	0.804585	2.01E-49	-1.69E-46
MS.gene050812	MS.gene63538	0.805698	1.18E-49	-1.69E-46
MS.gene050950	MS.gene63538	0.806558	7.76E-50	-1.69E-46
MS.gene05101	MS.gene63538	0.846628	1.93E-59	-1.69E-46
MS.gene05105	MS.gene63538	0.923241	3.44E-89	-1.69E-46
MS.gene051228	MS.gene63538	0.802148	6.44E-49	-1.69E-46
MS.gene051387	MS.gene63538	0.811416	7.08E-51	-1.69E-46
MS.gene051471	MS.gene63538	0.823213	1.56E-53	-1.69E-46
MS.gene051582	MS.gene63538	0.827351	1.64E-54	-1.69E-46
MS.gene051605	MS.gene63538	0.842442	2.58E-58	-1.69E-46
MS.gene051666	MS.gene63538	0.847357	1.22E-59	-1.69E-46
MS.gene051858	MS.gene63538	0.805702	1.18E-49	-1.69E-46
MS.gene052078	MS.gene63538	0.828004	1.14E-54	-1.69E-46
MS.gene052188	MS.gene63538	0.841187	5.53E-58	-1.69E-46
MS.gene052253	MS.gene63538	0.852093	5.79E-61	-1.69E-46
MS.gene052271	MS.gene63538	0.824082	9.79E-54	-1.69E-46
MS.gene052290	MS.gene63538	0.839312	1.71E-57	-1.69E-46
MS.gene052310	MS.gene63538	0.879803	9.25E-70	-1.69E-46
MS.gene052322	MS.gene63538	0.825083	5.69E-54	-1.69E-46
MS.gene052326	MS.gene63538	0.809372	1.96E-50	-1.69E-46
MS.gene052340	MS.gene63538	0.857637	1.43E-62	-1.69E-46
MS.gene052421	MS.gene63538	0.843025	1.81E-58	-1.69E-46
MS.gene052481	MS.gene63538	0.847251	1.30E-59	-1.69E-46
MS.gene052484	MS.gene63538	0.831885	1.29E-55	-1.69E-46
MS.gene052485	MS.gene63538	0.849606	2.90E-60	-1.69E-46
MS.gene052982	MS.gene63538	0.821361	4.21E-53	-1.69E-46
MS.gene053009	MS.gene63538	0.85079	1.35E-60	-1.69E-46
MS.gene053103	MS.gene63538	0.860403	2.12E-63	-1.69E-46
MS.gene053104	MS.gene63538	0.85689	2.37E-62	-1.69E-46
MS.gene053207	MS.gene63538	0.810352	1.20E-50	-1.69E-46
MS.gene053209	MS.gene63538	0.881709	1.92E-70	-1.69E-46
MS.gene053213	MS.gene63538	0.831916	1.27E-55	-1.69E-46
MS.gene053286	MS.gene63538	0.815665	8.23E-52	-1.69E-46
MS.gene053322	MS.gene63538	0.849475	3.16E-60	-1.69E-46
MS.gene053557	MS.gene63538	0.827291	1.70E-54	-1.69E-46
MS.gene053574	MS.gene63538	0.839457	1.57E-57	-1.69E-46
MS.gene053836	MS.gene63538	0.804507	2.09E-49	-1.69E-46
MS.gene054376	MS.gene63538	0.860804	1.60E-63	-1.69E-46
MS.gene054642	MS.gene63538	0.805944	1.05E-49	-1.69E-46
MS.gene054802	MS.gene63538	0.812427	4.26E-51	-1.69E-46
MS.gene054803	MS.gene63538	0.809804	1.58E-50	-1.69E-46
MS.gene054804	MS.gene63538	0.813625	2.33E-51	-1.69E-46
MS.gene054927	MS.gene63538	0.80436	2.24E-49	-1.69E-46
MS.gene05505	MS.gene63538	0.854195	1.45E-61	-1.69E-46
MS.gene055116	MS.gene63538	0.939417	1.32E-99	-1.69E-46
MS.gene055212	MS.gene63538	0.818965	1.49E-52	-1.69E-46
MS.gene055254	MS.gene63538	0.834385	3.09E-56	-1.69E-46
MS.gene055277	MS.gene63538	0.847029	1.50E-59	-1.69E-46
MS.gene055289	MS.gene63538	0.824446	8.03E-54	-1.69E-46
MS.gene055397	MS.gene63538	0.831947	1.25E-55	-1.69E-46
MS.gene055422	MS.gene63538	0.815415	9.35E-52	-1.69E-46
MS.gene055428	MS.gene63538	0.824463	7.96E-54	-1.69E-46
MS.gene055499	MS.gene63538	0.84854	5.74E-60	-1.69E-46
MS.gene055722	MS.gene63538	0.852215	5.34E-61	-1.69E-46
MS.gene05592	MS.gene63538	0.850803	1.34E-60	-1.69E-46
MS.gene05602	MS.gene63538	0.811566	6.57E-51	-1.69E-46
MS.gene056191	MS.gene63538	0.87229	3.56E-67	-1.69E-46
MS.gene056374	MS.gene63538	0.801093	1.06E-48	-1.69E-46
MS.gene056407	MS.gene63538	0.848807	4.84E-60	-1.69E-46
MS.gene056410	MS.gene63538	0.825169	5.43E-54	-1.69E-46
MS.gene056511	MS.gene63538	0.84978	2.60E-60	-1.69E-46
MS.gene056609	MS.gene63538	0.826	3.45E-54	-1.69E-46
MS.gene056612	MS.gene63538	0.81619	6.28E-52	-1.69E-46
MS.gene056659	MS.gene63538	0.815211	1.04E-51	-1.69E-46
MS.gene056897	MS.gene63538	0.832725	8.03E-56	-1.69E-46
MS.gene057011	MS.gene63538	0.812159	4.88E-51	-1.69E-46
MS.gene05702	MS.gene63538	0.864135	1.51E-64	-1.69E-46
MS.gene057076	MS.gene63538	0.808523	2.97E-50	-1.69E-46
MS.gene057123	MS.gene63538	0.804179	2.45E-49	-1.69E-46
MS.gene057124	MS.gene63538	0.819753	9.84E-53	-1.69E-46
MS.gene057129	MS.gene63538	0.852869	3.48E-61	-1.69E-46
MS.gene057146	MS.gene63538	0.835158	1.98E-56	-1.69E-46
MS.gene057207	MS.gene63538	0.81619	6.28E-52	-1.69E-46
MS.gene057295	MS.gene63538	0.802406	5.70E-49	-1.69E-46
MS.gene057310	MS.gene63538	0.810435	1.15E-50	-1.69E-46
MS.gene057331	MS.gene63538	0.854167	1.47E-61	-1.69E-46
MS.gene05733	MS.gene63538	0.81688	4.40E-52	-1.69E-46
MS.gene057416	MS.gene63538	0.834915	2.27E-56	-1.69E-46
MS.gene057450	MS.gene63538	0.801191	1.01E-48	-1.69E-46
MS.gene057749	MS.gene63538	0.814737	1.32E-51	-1.69E-46
MS.gene057823	MS.gene63538	0.807439	5.05E-50	-1.69E-46
MS.gene058002	MS.gene63538	0.833728	4.52E-56	-1.69E-46
MS.gene058007	MS.gene63538	0.81102	8.63E-51	-1.69E-46
MS.gene058019	MS.gene63538	0.831086	2.04E-55	-1.69E-46
MS.gene058082	MS.gene63538	0.838025	3.67E-57	-1.69E-46
MS.gene05811	MS.gene63538	0.801116	1.05E-48	-1.69E-46
MS.gene058130	MS.gene63538	0.827742	1.32E-54	-1.69E-46
MS.gene058233	MS.gene63538	0.84485	5.85E-59	-1.69E-46
MS.gene058293	MS.gene63538	0.854325	1.33E-61	-1.69E-46
MS.gene058323	MS.gene63538	0.807283	5.45E-50	-1.69E-46
MS.gene058365	MS.gene63538	0.814068	1.86E-51	-1.69E-46
MS.gene058419	MS.gene63538	0.803276	3.77E-49	-1.69E-46
MS.gene058660	MS.gene63538	0.876659	1.17E-68	-1.69E-46
MS.gene058723	MS.gene63538	0.850495	1.64E-60	-1.69E-46
MS.gene058787	MS.gene63538	0.837905	3.94E-57	-1.69E-46
MS.gene058928	MS.gene63538	0.824184	9.26E-54	-1.69E-46
MS.gene05907	MS.gene63538	0.826547	2.56E-54	-1.69E-46
MS.gene059294	MS.gene63538	0.803468	3.44E-49	-1.69E-46
MS.gene059366	MS.gene63538	0.811839	5.73E-51	-1.69E-46
MS.gene059536	MS.gene63538	0.860166	2.50E-63	-1.69E-46
MS.gene059597	MS.gene63538	0.82125	4.46E-53	-1.69E-46
MS.gene059676	MS.gene63538	0.820497	6.65E-53	-1.69E-46
MS.gene059688	MS.gene63538	0.923384	2.85E-89	-1.69E-46
MS.gene059692	MS.gene63538	0.923613	2.11E-89	-1.69E-46
MS.gene059695	MS.gene63538	0.924623	5.50E-90	-1.69E-46
MS.gene059711	MS.gene63538	0.817413	3.34E-52	-1.69E-46
MS.gene059807	MS.gene63538	0.809374	1.95E-50	-1.69E-46
MS.gene05984	MS.gene63538	0.806029	1.00E-49	-1.69E-46
MS.gene059894	MS.gene63538	0.819909	9.06E-53	-1.69E-46
MS.gene059902	MS.gene63538	0.808198	3.48E-50	-1.69E-46
MS.gene059960	MS.gene63538	0.848583	5.59E-60	-1.69E-46
MS.gene059974	MS.gene63538	0.813215	2.86E-51	-1.69E-46
MS.gene06037	MS.gene63538	0.840633	7.73E-58	-1.69E-46
MS.gene060403	MS.gene63538	0.849807	2.55E-60	-1.69E-46
MS.gene060804	MS.gene63538	0.885796	5.97E-72	-1.69E-46
MS.gene060818	MS.gene63538	0.802012	6.87E-49	-1.69E-46
MS.gene060835	MS.gene63538	0.812348	4.44E-51	-1.69E-46
MS.gene060837	MS.gene63538	0.831046	2.08E-55	-1.69E-46
MS.gene06092	MS.gene63538	0.801474	8.85E-49	-1.69E-46
MS.gene061041	MS.gene63538	0.855329	6.79E-62	-1.69E-46
MS.gene061049	MS.gene63538	0.865707	4.86E-65	-1.69E-46
MS.gene061051	MS.gene63538	-0.837908	3.94E-57	-1.69E-46
MS.gene061154	MS.gene63538	0.820573	6.39E-53	-1.69E-46
MS.gene061200	MS.gene63538	0.8457	3.45E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63538.t1	MTR_3g010000	91.358	162	2	1	1	162	1	150	1.43e-104	296

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63538.t1	AT1G55670	70.303	165	41	3	1	162	1	160	1.38e-77	228

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTATGATCCAAGTTTCTT+TGG	0.281064	3.3:-5102718	MS.gene63538:CDS
TTGCTCAGGTAAACCTTGTT+TGG	0.325278	3.3:+5102900	None:intergenic
TATTGTTGCTTACTATATCT+TGG	0.334380	3.3:-5102755	MS.gene63538:CDS
GTTAAAACCAACAGGATCAT+TGG	0.338510	3.3:+5102813	None:intergenic
CCTTGGAAGGACAAGCTTGA+TGG	0.371656	3.3:+5103099	None:intergenic
CAAGGTTTACCTGAGCAAAA+TGG	0.413142	3.3:-5102895	MS.gene63538:CDS
TCAACAAGGTTAAAACCAAC+AGG	0.475786	3.3:+5102805	None:intergenic
CCATCAAGCTTGTCCTTCCA+AGG	0.477711	3.3:-5103099	MS.gene63538:CDS
TTGTGAGAGGGTTGAAACCT+TGG	0.480579	3.3:+5103082	None:intergenic
CTTAAATCCAATGATCCTGT+TGG	0.489692	3.3:-5102820	MS.gene63538:CDS
AAATATCAACCAAAGAAACT+TGG	0.505006	3.3:+5102709	None:intergenic
CTTAAGCTTATCACAAGTGA+AGG	0.506695	3.3:+5102985	None:intergenic
AGCTGGTGACAAACGTGCTA+AGG	0.525842	3.3:-5102857	MS.gene63538:CDS
TAATGCATGAGTTGATATCA+TGG	0.550417	3.3:+5103161	None:intergenic
ATCTTGGCTACTTCTAGCAA+TGG	0.561786	3.3:-5102739	MS.gene63538:CDS
AGAGAAAATGTAGCCAAACA+AGG	0.605293	3.3:-5102913	MS.gene63538:CDS
AAGGTTTCAACCCTCTCACA+AGG	0.615424	3.3:-5103080	MS.gene63538:CDS
CTTGTGATAAGCTTAAGCAC+TGG	0.620138	3.3:-5102979	MS.gene63538:CDS
GAGAGGGTTGAAACCTTGGA+AGG	0.623355	3.3:+5103086	None:intergenic
TTAAGCTTATCACAAGTGAA+GGG	0.638403	3.3:+5102986	None:intergenic
ATGAGTTGATATCATGGATG+AGG	0.640889	3.3:+5103167	None:intergenic
GGTGTGACACACTTTGAAGC+TGG	0.654419	3.3:-5102874	MS.gene63538:CDS
CCCAAGCCAAAACATCAACA+AGG	0.663918	3.3:+5102791	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTGTTGCTTACTATATCT+TGG	-	chr3.3:5103139-5103158	MS.gene63538:CDS	25.0%
!!	TTTTAACCTTGTTGATGTTT+TGG	-	chr3.3:5103097-5103116	MS.gene63538:CDS	25.0%
	ACCTTCCTAAAAACAATGAA+AGG	+	chr3.3:5102941-5102960	None:intergenic	30.0%
	TTAAGCTTATCACAAGTGAA+GGG	+	chr3.3:5102911-5102930	None:intergenic	30.0%
!	CTTGAAAATCTCTTTTTGCT+TGG	+	chr3.3:5102864-5102883	None:intergenic	30.0%
!!	AGTTTTGTGTTTTTGAATGG+TGG	+	chr3.3:5102763-5102782	None:intergenic	30.0%
!!	GTGAGTTTTGTGTTTTTGAA+TGG	+	chr3.3:5102766-5102785	None:intergenic	30.0%
!!	TAATGCATGAGTTGATATCA+TGG	+	chr3.3:5102736-5102755	None:intergenic	30.0%
	AGAGAAAATGTAGCCAAACA+AGG	-	chr3.3:5102981-5103000	MS.gene63538:CDS	35.0%
	CTTAAATCCAATGATCCTGT+TGG	-	chr3.3:5103074-5103093	MS.gene63538:CDS	35.0%
	CTTAAGCTTATCACAAGTGA+AGG	+	chr3.3:5102912-5102931	None:intergenic	35.0%
	GGTTATGATCCAAGTTTCTT+TGG	-	chr3.3:5103176-5103195	MS.gene63538:CDS	35.0%
	GTTAAAACCAACAGGATCAT+TGG	+	chr3.3:5103084-5103103	None:intergenic	35.0%
	TCAACAAGGTTAAAACCAAC+AGG	+	chr3.3:5103092-5103111	None:intergenic	35.0%
!	GTTTGGCTACATTTTCTCTT+TGG	+	chr3.3:5102980-5102999	None:intergenic	35.0%
!!	ATGAGTTGATATCATGGATG+AGG	+	chr3.3:5102730-5102749	None:intergenic	35.0%
!!!	ACTGGCCTTTCATTGTTTTT+AGG	-	chr3.3:5102933-5102952	MS.gene63538:CDS	35.0%
!!!	GCCTTTCATTGTTTTTAGGA+AGG	-	chr3.3:5102937-5102956	MS.gene63538:CDS	35.0%
	ATCTTGGCTACTTCTAGCAA+TGG	-	chr3.3:5103155-5103174	MS.gene63538:CDS	40.0%
	CAAGGTTTACCTGAGCAAAA+TGG	-	chr3.3:5102999-5103018	MS.gene63538:CDS	40.0%
	CTTGTGATAAGCTTAAGCAC+TGG	-	chr3.3:5102915-5102934	MS.gene63538:CDS	40.0%
	TTGCTCAGGTAAACCTTGTT+TGG	+	chr3.3:5102997-5103016	None:intergenic	40.0%
!	ACTTTTGTTGACCTTGTGAG+AGG	+	chr3.3:5102828-5102847	None:intergenic	40.0%
!	CTTTTGTTGACCTTGTGAGA+GGG	+	chr3.3:5102827-5102846	None:intergenic	40.0%
!!!	ACCTTGTTGATGTTTTGGCT+TGG	-	chr3.3:5103102-5103121	MS.gene63538:CDS	40.0%
!!!	CCTTGTTGATGTTTTGGCTT+GGG	-	chr3.3:5103103-5103122	MS.gene63538:CDS	40.0%
!!!	CTTGTTGATGTTTTGGCTTG+GGG	-	chr3.3:5103104-5103123	MS.gene63538:CDS	40.0%
	AAGGTTTCAACCCTCTCACA+AGG	-	chr3.3:5102814-5102833	MS.gene63538:CDS	45.0%
	CCCAAGCCAAAACATCAACA+AGG	+	chr3.3:5103106-5103125	None:intergenic	45.0%
	TTGTGAGAGGGTTGAAACCT+TGG	+	chr3.3:5102815-5102834	None:intergenic	45.0%
!	TGTGTCACACCATTTTGCTC+AGG	+	chr3.3:5103011-5103030	None:intergenic	45.0%
!!!	GTTTTGGCTTGGGGTTCTTT+AGG	-	chr3.3:5103113-5103132	MS.gene63538:CDS	45.0%
	AGCTGGTGACAAACGTGCTA+AGG	-	chr3.3:5103037-5103056	MS.gene63538:CDS	50.0%
	CCATCAAGCTTGTCCTTCCA+AGG	-	chr3.3:5102795-5102814	MS.gene63538:CDS	50.0%
	CCTTGGAAGGACAAGCTTGA+TGG	+	chr3.3:5102798-5102817	None:intergenic	50.0%
	GAGAGGGTTGAAACCTTGGA+AGG	+	chr3.3:5102811-5102830	None:intergenic	50.0%
!!	GGTGTGACACACTTTGAAGC+TGG	-	chr3.3:5103020-5103039	MS.gene63538:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	5102714	5103202	5102714	ID=MS.gene63538
chr3.3	mRNA	5102714	5103202	5102714	ID=MS.gene63538.t1;Parent=MS.gene63538
chr3.3	exon	5102714	5103202	5102714	ID=MS.gene63538.t1.exon1;Parent=MS.gene63538.t1
chr3.3	CDS	5102714	5103202	5102714	ID=cds.MS.gene63538.t1;Parent=MS.gene63538.t1

Gene Sequence

>MS.gene63538

ATGGCAGCTTCAGCCTCATCCATGATATCAACTCATGCATTAACAACCACCATTCAAAAACACAAAACTCACAATCTCAAGCCATCAAGCTTGTCCTTCCAAGGTTTCAACCCTCTCACAAGGTCAACAAAAGTCAACACATTCACACCAAGCAAAAAGAGATTTTCAAGTGTTGTTGTCAAAGCTGAACTCAACCCTTCACTTGTGATAAGCTTAAGCACTGGCCTTTCATTGTTTTTAGGAAGGTTTGTGTTTTTCAACTTCCAAAGAGAAAATGTAGCCAAACAAGGTTTACCTGAGCAAAATGGTGTGACACACTTTGAAGCTGGTGACAAACGTGCTAAGGAATATGTTAGCTTGCTTAAATCCAATGATCCTGTTGGTTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTTTAGGTCATATTGTTGCTTACTATATCTTGGCTACTTCTAGCAATGGTTATGATCCAAGTTTCTTTGGTTGA

Protein sequence

>MS.gene63538.t1

MAASASSMISTHALTTTIQKHKTHNLKPSSLSFQGFNPLTRSTKVNTFTPSKKRFSSVVVKAELNPSLVISLSTGLSLFLGRFVFFNFQRENVAKQGLPEQNGVTHFEAGDKRAKEYVSLLKSNDPVGFNLVDVLAWGSLGHIVAYYILATSSNGYDPSFFG