AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71267

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71267.t1 MTR_0059s0060 93.000 100 6 1 1 100 26 124 3.43e-42 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAGAGAGGAAGAAGGTTC+TGG 0.298442 4.2:-64415282 None:intergenic
AGAAGAAGGAGAAAGGTCTT+GGG 0.330843 4.2:-64415246 None:intergenic
CATTACTTCTCAAGAGTGAT+AGG 0.340890 4.2:+64415489 MS.gene71267:CDS
GGAGAGAGGAAGAAGGTTCT+GGG 0.357732 4.2:-64415281 None:intergenic
GAGAAGAAGGAGAAAGGTCT+TGG 0.368226 4.2:-64415247 None:intergenic
AATGCAGCTTTCCCTTTCTT+AGG 0.383121 4.2:-64415470 None:intergenic
GTTACTACTAAGCCTAAGAA+AGG 0.488925 4.2:+64415458 MS.gene71267:CDS
GTTAGATTAACCGACTTAGT+AGG 0.492831 4.2:-64415317 None:intergenic
GGAAAAGAACGGAGAGAAGA+AGG 0.505919 4.2:-64415260 None:intergenic
GAAGAAGGAGAAAGGTCTTG+GGG 0.510778 4.2:-64415245 None:intergenic
TTAATCTAACCTACCAGTCT+AGG 0.519742 4.2:+64415330 MS.gene71267:CDS
GTAAAATCGAAGACCTAGAC+TGG 0.533354 4.2:-64415343 None:intergenic
AAGAAGGAGAAAGGTCTTGG+GGG 0.541318 4.2:-64415244 None:intergenic
GCTTCAAAACGAGGGGAGAG+AGG 0.550446 4.2:-64415295 None:intergenic
AACGGAGAGAAGAAGGAGAA+AGG 0.551129 4.2:-64415253 None:intergenic
AACGAGGGGAGAGAGGAAGA+AGG 0.573710 4.2:-64415288 None:intergenic
GAGAAGGATGGTGTTGATGA+TGG 0.575832 4.2:+64415413 MS.gene71267:CDS
ATTACTTCTCAAGAGTGATA+GGG 0.582083 4.2:+64415490 MS.gene71267:CDS
TTACTACTAAGCCTAAGAAA+GGG 0.585009 4.2:+64415459 MS.gene71267:CDS
AACTGTTGATGAAGATGAGA+AGG 0.595375 4.2:+64415397 MS.gene71267:CDS
GGGGTGAGAATAGAAGAAGA+GGG 0.598380 4.2:-64415224 None:intergenic
ACTTAGTAGGCTTCAAAACG+AGG 0.603839 4.2:-64415304 None:intergenic
AATCGAAGACCTAGACTGGT+AGG 0.609515 4.2:-64415339 None:intergenic
GGGTGAGAATAGAAGAAGAG+GGG 0.614380 4.2:-64415223 None:intergenic
AGAAGGAGAAAGGTCTTGGG+GGG 0.616732 4.2:-64415243 None:intergenic
AGAAGGTTCTGGGAAAAGAA+CGG 0.632360 4.2:-64415271 None:intergenic
CTTAGTAGGCTTCAAAACGA+GGG 0.651809 4.2:-64415303 None:intergenic
AGCAACATCAGCAGTGACAG+CGG 0.657412 4.2:-64415372 None:intergenic
GTTGATGAAGATGAGAAGGA+TGG 0.658305 4.2:+64415401 MS.gene71267:CDS
TTAGTAGGCTTCAAAACGAG+GGG 0.672051 4.2:-64415302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTACTACTAAGCCTAAGAAA+GGG + chr4.2:64415459-64415478 MS.gene71267:CDS 30.0%
CATTACTTCTCAAGAGTGAT+AGG + chr4.2:64415489-64415508 MS.gene71267:CDS 35.0%
GTTACTACTAAGCCTAAGAA+AGG + chr4.2:64415458-64415477 MS.gene71267:CDS 35.0%
TTAATCTAACCTACCAGTCT+AGG + chr4.2:64415330-64415349 MS.gene71267:CDS 35.0%
! AACTGTTGATGAAGATGAGA+AGG + chr4.2:64415397-64415416 MS.gene71267:CDS 35.0%
! GTTAGATTAACCGACTTAGT+AGG - chr4.2:64415320-64415339 None:intergenic 35.0%
AATGCAGCTTTCCCTTTCTT+AGG - chr4.2:64415473-64415492 None:intergenic 40.0%
ACTTAGTAGGCTTCAAAACG+AGG - chr4.2:64415307-64415326 None:intergenic 40.0%
AGAAGAAGGAGAAAGGTCTT+GGG - chr4.2:64415249-64415268 None:intergenic 40.0%
CTTAGTAGGCTTCAAAACGA+GGG - chr4.2:64415306-64415325 None:intergenic 40.0%
GTAAAATCGAAGACCTAGAC+TGG - chr4.2:64415346-64415365 None:intergenic 40.0%
TTAGTAGGCTTCAAAACGAG+GGG - chr4.2:64415305-64415324 None:intergenic 40.0%
! GTTGATGAAGATGAGAAGGA+TGG + chr4.2:64415401-64415420 MS.gene71267:CDS 40.0%
!! AGAAGGTTCTGGGAAAAGAA+CGG - chr4.2:64415274-64415293 None:intergenic 40.0%
!!! CGTTTTGAAGCCTACTAAGT+CGG + chr4.2:64415307-64415326 MS.gene71267:CDS 40.0%
AACGGAGAGAAGAAGGAGAA+AGG - chr4.2:64415256-64415275 None:intergenic 45.0%
AAGAAGGAGAAAGGTCTTGG+GGG - chr4.2:64415247-64415266 None:intergenic 45.0%
AATCGAAGACCTAGACTGGT+AGG - chr4.2:64415342-64415361 None:intergenic 45.0%
GAAGAAGGAGAAAGGTCTTG+GGG - chr4.2:64415248-64415267 None:intergenic 45.0%
GAGAAGAAGGAGAAAGGTCT+TGG - chr4.2:64415250-64415269 None:intergenic 45.0%
GGAAAAGAACGGAGAGAAGA+AGG - chr4.2:64415263-64415282 None:intergenic 45.0%
GGGGTGAGAATAGAAGAAGA+GGG - chr4.2:64415227-64415246 None:intergenic 45.0%
GGGTGAGAATAGAAGAAGAG+GGG - chr4.2:64415226-64415245 None:intergenic 45.0%
!! GAGAAGGATGGTGTTGATGA+TGG + chr4.2:64415413-64415432 MS.gene71267:CDS 45.0%
AGAAGGAGAAAGGTCTTGGG+GGG - chr4.2:64415246-64415265 None:intergenic 50.0%
AGCAACATCAGCAGTGACAG+CGG - chr4.2:64415375-64415394 None:intergenic 50.0%
GGAGAGAGGAAGAAGGTTCT+GGG - chr4.2:64415284-64415303 None:intergenic 50.0%
GGGGGTGAGAATAGAAGAAG+AGG - chr4.2:64415228-64415247 None:intergenic 50.0%
AACGAGGGGAGAGAGGAAGA+AGG - chr4.2:64415291-64415310 None:intergenic 55.0%
GCTTCAAAACGAGGGGAGAG+AGG - chr4.2:64415298-64415317 None:intergenic 55.0%
GGGAGAGAGGAAGAAGGTTC+TGG - chr4.2:64415285-64415304 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 64415212 64415511 64415212 ID=MS.gene71267
chr4.2 mRNA 64415212 64415511 64415212 ID=MS.gene71267.t1;Parent=MS.gene71267
chr4.2 exon 64415212 64415511 64415212 ID=MS.gene71267.t1.exon1;Parent=MS.gene71267.t1
chr4.2 CDS 64415212 64415511 64415212 ID=cds.MS.gene71267.t1;Parent=MS.gene71267.t1
Gene Sequence

>MS.gene71267

ATGCTAACACACCCCTCTTCTTCTATTCTCACCCCCCAAGACCTTTCTCCTTCTTCTCTCCGTTCTTTTCCCAGAACCTTCTTCCTCTCTCCCCTCGTTTTGAAGCCTACTAAGTCGGTTAATCTAACCTACCAGTCTAGGTCTTCGATTTTACTTGCTTCCGCTGTCACTGCTGATGTTGCTGAAACTGTTGATGAAGATGAGAAGGATGGTGTTGATGATGGTGTTGCTGAAACTGTGACTGTTGTTACTACTAAGCCTAAGAAAGGGAAAGCTGCATTACTTCTCAAGAGTGATAGG
Protein sequence

>MS.gene71267.t1

MLTHPSSSILTPQDLSPSSLRSFPRTFFLSPLVLKPTKSVNLTYQSRSSILLASAVTADVAETVDEDEKDGVDDGVAETVTVVTTKPKKGKAALLLKSDR