AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1086590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086590 MtrunA17_CPg0492431 97.015 67 2 0 141 207 1 67 7.24e-38 132
Msa1086590 MtrunA17_Chr3g0090851 97.015 67 2 0 141 207 1 67 7.24e-38 132
Msa1086590 MtrunA17_Chr4g0016091 97.015 67 2 0 141 207 1 67 7.24e-38 132
Msa1086590 MtrunA17_CPg0492421 59.223 103 32 4 27 120 50 151 4.77e-29 106
Msa1086590 MtrunA17_Chr4g0016101 59.223 103 32 4 27 120 50 151 4.77e-29 106
Msa1086590 MtrunA17_Chr3g0090841 59.223 103 32 4 27 120 50 151 4.77e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1086590 ATCG00900.1 65.347 101 27 3 27 120 50 149 9.59e-36 123
Msa1086590 ATCG01240.1 65.347 101 27 3 27 120 50 149 9.59e-36 123

Find 30 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCGTGACATGAGCGTAAA+AGG 0.177996 7_2:-87461635 None:intergenic
ACTAATCGATTACGATAAAT+TGG 0.238141 7_2:-87461692 None:intergenic
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 7_2:+87461897 Msa1086590:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 7_2:+87461971 Msa1086590:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 7_2:+87461998 Msa1086590:CDS
TTTGGCAGCATCCACTAATT+CGG 0.375526 7_2:-87462009 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 7_2:-87461823 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 7_2:-87461816 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 7_2:+87461867 Msa1086590:CDS
GTATGGCATCGCCATTCCTT+TGG 0.449289 7_2:-87462027 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 7_2:-87461906 None:intergenic
ATGGCGATGCCATACGCAAA+AGG 0.523996 7_2:+87462035 Msa1086590:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 7_2:+87461931 Msa1086590:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 7_2:+87461960 Msa1086590:intron
TTGTGTGGATCCTATTTCAA+CGG 0.548545 7_2:-87461907 None:intergenic
GCCGTGACATGAGCGTAAAA+GGG 0.558201 7_2:-87461634 None:intergenic
ATCGAAAAGAAGCAAATGAA+AGG 0.561694 7_2:-87462397 None:intergenic
AAAGGAAGAGACTCATAGAA+TGG 0.565489 7_2:+87462053 Msa1086590:CDS
TCATGGGTTCTTCTCTCCAT+CGG 0.567612 7_2:-87462566 None:intergenic
TAACGGCGCTGTTGTTCCGA+TGG 0.571426 7_2:+87462550 Msa1086590:CDS
AGTGGATGCTGCCAAAGGAA+TGG 0.579178 7_2:+87462016 Msa1086590:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 7_2:+87462059 Msa1086590:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 7_2:-87461978 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 7_2:-87461943 None:intergenic
GAATTAGTGGATGCTGCCAA+AGG 0.621520 7_2:+87462011 Msa1086590:CDS
TCGATTACGATAAATTGGAT+CGG 0.627987 7_2:-87461687 None:intergenic
AAGTTTAGTAATGAGCATAA+CGG 0.644237 7_2:+87462533 Msa1086590:CDS
TTTACGCTCATGTCACGGCG+AGG 0.672550 7_2:+87461638 Msa1086590:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 7_2:+87461827 Msa1086590:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 7_2:+87461909 Msa1086590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAGAGGAATATT+TGG + chr7_2:87462279-87462298 Msa1086590:intron 15.0%
!! AATAAATAAAGAGGAATATT+TGG + chr7_2:87462279-87462298 Msa1086590:intron 15.0%
!! AGAATCAATAGAAAAAGAAT+CGG + chr7_2:87462177-87462196 Msa1086590:intron 20.0%
!! TTGCTTAAGAATAAATAAAG+AGG + chr7_2:87462270-87462289 Msa1086590:intron 20.0%
!!! AGGAATATTTGGTATTTTTT+TGG + chr7_2:87462290-87462309 Msa1086590:intron 20.0%
!! AGAATCAATAGAAAAAGAAT+CGG + chr7_2:87462177-87462196 Msa1086590:intron 20.0%
!! TTGCTTAAGAATAAATAAAG+AGG + chr7_2:87462270-87462289 Msa1086590:intron 20.0%
!!! AGGAATATTTGGTATTTTTT+TGG + chr7_2:87462290-87462309 Msa1086590:intron 20.0%
! ACTAATCGATTACGATAAAT+TGG - chr7_2:87461695-87461714 None:intergenic 25.0%
!! TTTTTCCGTGTTTCATAATA+CGG - chr7_2:87461732-87461751 None:intergenic 25.0%
!! GATTCTTTTCCAATTATGTA+TGG - chr7_2:87462163-87462182 None:intergenic 25.0%
! GAAGAATTAGACCAAAAATT+AGG - chr7_2:87462449-87462468 None:intergenic 25.0%
! AAGTTTAGTAATGAGCATAA+CGG + chr7_2:87462533-87462552 Msa1086590:CDS 25.0%
! ACTAATCGATTACGATAAAT+TGG - chr7_2:87461695-87461714 None:intergenic 25.0%
!! TTTTTCCGTGTTTCATAATA+CGG - chr7_2:87461732-87461751 None:intergenic 25.0%
!! GATTCTTTTCCAATTATGTA+TGG - chr7_2:87462163-87462182 None:intergenic 25.0%
! GAAGAATTAGACCAAAAATT+AGG - chr7_2:87462449-87462468 None:intergenic 25.0%
! AAGTTTAGTAATGAGCATAA+CGG + chr7_2:87462533-87462552 Msa1086590:CDS 25.0%
! ATAAATTGGATCGGATTTTG+CGG - chr7_2:87461681-87461700 None:intergenic 30.0%
TCGATTACGATAAATTGGAT+CGG - chr7_2:87461690-87461709 None:intergenic 30.0%
GTTAACCGTATTATGAAACA+CGG + chr7_2:87461724-87461743 Msa1086590:intron 30.0%
GAAACACGGAAAAAAATCAT+TGG + chr7_2:87461738-87461757 Msa1086590:intron 30.0%
ATTATCTATCGAGCTATGAA+AGG + chr7_2:87461769-87461788 Msa1086590:intron 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG - chr7_2:87461859-87461878 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG + chr7_2:87461998-87462017 Msa1086590:CDS 30.0%
ACATAGACCTATGTTATGTA+TGG + chr7_2:87462135-87462154 Msa1086590:intron 30.0%
TATGGATCCATACATAACAT+AGG - chr7_2:87462145-87462164 None:intergenic 30.0%
TGTATGGATCCATACATAAT+TGG + chr7_2:87462151-87462170 Msa1086590:intron 30.0%
ATCGAAAAGAAGCAAATGAA+AGG - chr7_2:87462400-87462419 None:intergenic 30.0%
! TTTCATTTGCTTCTTTTCGA+TGG + chr7_2:87462399-87462418 Msa1086590:CDS 30.0%
!!! CGATGGAAGTTTTATTTTTC+CGG + chr7_2:87462416-87462435 Msa1086590:CDS 30.0%
CCAAAAATTAGGATCAATTC+CGG - chr7_2:87462438-87462457 None:intergenic 30.0%
! AGATAGATCTTTTTGATCAG+AGG - chr7_2:87462490-87462509 None:intergenic 30.0%
CTGATCAAAAAGATCTATCT+TGG + chr7_2:87462490-87462509 Msa1086590:CDS 30.0%
! ATAAATTGGATCGGATTTTG+CGG - chr7_2:87461681-87461700 None:intergenic 30.0%
TCGATTACGATAAATTGGAT+CGG - chr7_2:87461690-87461709 None:intergenic 30.0%
GTTAACCGTATTATGAAACA+CGG + chr7_2:87461724-87461743 Msa1086590:intron 30.0%
GAAACACGGAAAAAAATCAT+TGG + chr7_2:87461738-87461757 Msa1086590:intron 30.0%
ATTATCTATCGAGCTATGAA+AGG + chr7_2:87461769-87461788 Msa1086590:intron 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG - chr7_2:87461859-87461878 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG + chr7_2:87461998-87462017 Msa1086590:CDS 30.0%
ACATAGACCTATGTTATGTA+TGG + chr7_2:87462135-87462154 Msa1086590:intron 30.0%
TATGGATCCATACATAACAT+AGG - chr7_2:87462145-87462164 None:intergenic 30.0%
TGTATGGATCCATACATAAT+TGG + chr7_2:87462151-87462170 Msa1086590:intron 30.0%
ATCGAAAAGAAGCAAATGAA+AGG - chr7_2:87462400-87462419 None:intergenic 30.0%
! TTTCATTTGCTTCTTTTCGA+TGG + chr7_2:87462399-87462418 Msa1086590:CDS 30.0%
!!! CGATGGAAGTTTTATTTTTC+CGG + chr7_2:87462416-87462435 Msa1086590:CDS 30.0%
CCAAAAATTAGGATCAATTC+CGG - chr7_2:87462438-87462457 None:intergenic 30.0%
! AGATAGATCTTTTTGATCAG+AGG - chr7_2:87462490-87462509 None:intergenic 30.0%
CTGATCAAAAAGATCTATCT+TGG + chr7_2:87462490-87462509 Msa1086590:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG - chr7_2:87461826-87461845 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG + chr7_2:87461826-87461845 Msa1086590:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG - chr7_2:87461858-87461877 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG - chr7_2:87461910-87461929 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG + chr7_2:87462053-87462072 Msa1086590:CDS 35.0%
! ATTTTCGTTAATCCATGAGC+AGG + chr7_2:87462100-87462119 Msa1086590:intron 35.0%
CGAAGACGAAATAGAAATGA+TGG + chr7_2:87462224-87462243 Msa1086590:intron 35.0%
! CCGGAATTGATCCTAATTTT+TGG + chr7_2:87462435-87462454 Msa1086590:CDS 35.0%
ACGTATTGCTTGACGTAAAA+CGG - chr7_2:87461826-87461845 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG + chr7_2:87461826-87461845 Msa1086590:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG - chr7_2:87461858-87461877 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG - chr7_2:87461910-87461929 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG + chr7_2:87462053-87462072 Msa1086590:CDS 35.0%
! ATTTTCGTTAATCCATGAGC+AGG + chr7_2:87462100-87462119 Msa1086590:intron 35.0%
CGAAGACGAAATAGAAATGA+TGG + chr7_2:87462224-87462243 Msa1086590:intron 35.0%
! CCGGAATTGATCCTAATTTT+TGG + chr7_2:87462435-87462454 Msa1086590:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG + chr7_2:87461825-87461844 Msa1086590:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG + chr7_2:87461827-87461846 Msa1086590:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG + chr7_2:87461867-87461886 Msa1086590:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG + chr7_2:87461897-87461916 Msa1086590:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG - chr7_2:87461909-87461928 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG + chr7_2:87461909-87461928 Msa1086590:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG + chr7_2:87461939-87461958 Msa1086590:intron 40.0%
! TATTTCGACCTGGACGTTTT+CGG - chr7_2:87461971-87461990 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG + chr7_2:87461971-87461990 Msa1086590:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG - chr7_2:87461981-87462000 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG - chr7_2:87462012-87462031 None:intergenic 40.0%
GTGTATATAGATCCTGCTCA+TGG - chr7_2:87462115-87462134 None:intergenic 40.0%
! ATGGCTCAACTAAGCACTTT+TGG + chr7_2:87462243-87462262 Msa1086590:intron 40.0%
! TGGCTCAACTAAGCACTTTT+GGG + chr7_2:87462244-87462263 Msa1086590:intron 40.0%
! GTTTTACGTCAAGCAATACG+TGG + chr7_2:87461825-87461844 Msa1086590:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG + chr7_2:87461827-87461846 Msa1086590:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG + chr7_2:87461867-87461886 Msa1086590:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG + chr7_2:87461897-87461916 Msa1086590:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG - chr7_2:87461909-87461928 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG + chr7_2:87461909-87461928 Msa1086590:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG + chr7_2:87461939-87461958 Msa1086590:intron 40.0%
! TATTTCGACCTGGACGTTTT+CGG - chr7_2:87461971-87461990 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG + chr7_2:87461971-87461990 Msa1086590:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG - chr7_2:87461981-87462000 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG - chr7_2:87462012-87462031 None:intergenic 40.0%
GTGTATATAGATCCTGCTCA+TGG - chr7_2:87462115-87462134 None:intergenic 40.0%
! ATGGCTCAACTAAGCACTTT+TGG + chr7_2:87462243-87462262 Msa1086590:intron 40.0%
! TGGCTCAACTAAGCACTTTT+GGG + chr7_2:87462244-87462263 Msa1086590:intron 40.0%
! TCCCTTTTACGCTCATGTCA+CGG + chr7_2:87461633-87461652 Msa1086590:CDS 45.0%
GCTTGACGTAAAACGGATAG+TGG - chr7_2:87461819-87461838 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG + chr7_2:87461931-87461950 Msa1086590:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG - chr7_2:87461946-87461965 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG - chr7_2:87461970-87461989 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG + chr7_2:87462011-87462030 Msa1086590:CDS 45.0%
! GAGTCTCTTCCTTTTGCGTA+TGG - chr7_2:87462047-87462066 None:intergenic 45.0%
AGAGACTCATAGAATGGCAG+AGG + chr7_2:87462059-87462078 Msa1086590:CDS 45.0%
! GGCTCAACTAAGCACTTTTG+GGG + chr7_2:87462245-87462264 Msa1086590:intron 45.0%
TCATGGGTTCTTCTCTCCAT+CGG - chr7_2:87462569-87462588 None:intergenic 45.0%
! TCCCTTTTACGCTCATGTCA+CGG + chr7_2:87461633-87461652 Msa1086590:CDS 45.0%
GCTTGACGTAAAACGGATAG+TGG - chr7_2:87461819-87461838 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG + chr7_2:87461931-87461950 Msa1086590:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG - chr7_2:87461946-87461965 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG - chr7_2:87461970-87461989 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG + chr7_2:87462011-87462030 Msa1086590:CDS 45.0%
! GAGTCTCTTCCTTTTGCGTA+TGG - chr7_2:87462047-87462066 None:intergenic 45.0%
AGAGACTCATAGAATGGCAG+AGG + chr7_2:87462059-87462078 Msa1086590:CDS 45.0%
! GGCTCAACTAAGCACTTTTG+GGG + chr7_2:87462245-87462264 Msa1086590:intron 45.0%
TCATGGGTTCTTCTCTCCAT+CGG - chr7_2:87462569-87462588 None:intergenic 45.0%
GCCGTGACATGAGCGTAAAA+GGG - chr7_2:87461637-87461656 None:intergenic 50.0%
! GGCAAGTGCTTTTCCTTGTG+TGG - chr7_2:87461925-87461944 None:intergenic 50.0%
AGTGGATGCTGCCAAAGGAA+TGG + chr7_2:87462016-87462035 Msa1086590:CDS 50.0%
GTATGGCATCGCCATTCCTT+TGG - chr7_2:87462030-87462049 None:intergenic 50.0%
ATGGCGATGCCATACGCAAA+AGG + chr7_2:87462035-87462054 Msa1086590:CDS 50.0%
GCCGTGACATGAGCGTAAAA+GGG - chr7_2:87461637-87461656 None:intergenic 50.0%
! GGCAAGTGCTTTTCCTTGTG+TGG - chr7_2:87461925-87461944 None:intergenic 50.0%
AGTGGATGCTGCCAAAGGAA+TGG + chr7_2:87462016-87462035 Msa1086590:CDS 50.0%
GTATGGCATCGCCATTCCTT+TGG - chr7_2:87462030-87462049 None:intergenic 50.0%
ATGGCGATGCCATACGCAAA+AGG + chr7_2:87462035-87462054 Msa1086590:CDS 50.0%
CGCCGTGACATGAGCGTAAA+AGG - chr7_2:87461638-87461657 None:intergenic 55.0%
TTTACGCTCATGTCACGGCG+AGG + chr7_2:87461638-87461657 Msa1086590:CDS 55.0%
TAACGGCGCTGTTGTTCCGA+TGG + chr7_2:87462550-87462569 Msa1086590:CDS 55.0%
CGCCGTGACATGAGCGTAAA+AGG - chr7_2:87461638-87461657 None:intergenic 55.0%
TTTACGCTCATGTCACGGCG+AGG + chr7_2:87461638-87461657 Msa1086590:CDS 55.0%
TAACGGCGCTGTTGTTCCGA+TGG + chr7_2:87462550-87462569 Msa1086590:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG + chr7_2:87461960-87461979 Msa1086590:intron 65.0%
GGCGCATCCCGAAAACGTCC+AGG + chr7_2:87461960-87461979 Msa1086590:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 87461625 87462590 87461625 ID=Msa1086590;Name=Msa1086590
chr7_2 mRNA 87461625 87462590 87461625 ID=Msa1086590-mRNA-1;Parent=Msa1086590;Name=Msa1086590-mRNA-1;_AED=0.30;_eAED=0.39;_QI=0|0|0|1|0|0|4|0|207
chr7_2 exon 87461625 87461701 87461625 ID=Msa1086590-mRNA-1:exon:23827;Parent=Msa1086590-mRNA-1
chr7_2 exon 87461791 87461952 87461791 ID=Msa1086590-mRNA-1:exon:23828;Parent=Msa1086590-mRNA-1
chr7_2 exon 87461961 87462105 87461961 ID=Msa1086590-mRNA-1:exon:23829;Parent=Msa1086590-mRNA-1
chr7_2 exon 87462354 87462590 87462354 ID=Msa1086590-mRNA-1:exon:23830;Parent=Msa1086590-mRNA-1
chr7_2 CDS 87461625 87461701 87461625 ID=Msa1086590-mRNA-1:cds;Parent=Msa1086590-mRNA-1
chr7_2 CDS 87461791 87461952 87461791 ID=Msa1086590-mRNA-1:cds;Parent=Msa1086590-mRNA-1
chr7_2 CDS 87461961 87462105 87461961 ID=Msa1086590-mRNA-1:cds;Parent=Msa1086590-mRNA-1
chr7_2 CDS 87462354 87462590 87462354 ID=Msa1086590-mRNA-1:cds;Parent=Msa1086590-mRNA-1
Gene Sequence

>Msa1086590

ATGGATTCTCCCTTTTACGCTCATGTCACGGCGAGGTACTGCAGAAAAAAAAACCGCAAAATCCGATCCAATTTATCGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGAATGGCGATGCCATACGCAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCAATGAAAATCTCATTCTCGAGTCTACGAGAATTTTTATGAAAGCCTTTCATTTGCTTCTTTTCGATGGAAGTTTTATTTTTCCGGAATTGATCCTAATTTTTGGTCTAATTCTTCTTCTGATGATTGATTTAACCTCTGATCAAAAAGATCTATCTTGGTTCTATTTTATCTCTTCAACAAGTTTAGTAATGAGCATAACGGCGCTGTTGTTCCGATGGAGAGAAGAACCCATGATT

Protein sequence

>Msa1086590

MDSPFYAHVTARYCRKKNRKIRSNLSIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWRIPKTSRSKYGFQIKFRISGCCQRNGDAIRKRKRLIEWQRQIELLHIFNENLILESTRIFMKAFHLLLFDGSFIFPELILIFGLILLLMIDLTSDQKDLSWFYFISSTSLVMSITALLFRWREEPMI