AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365960 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1365960 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1365960 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1365960 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa1365960 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365960 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1365960 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1365960 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa1365960 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0018442:-50485 Msa1365960:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0018442:-50527 Msa1365960:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0018442:-50453 Msa1365960:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0018442:-50426 Msa1365960:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0018442:-50387 Msa1365960:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 tig0018442:+50415 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0018442:+50601 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0018442:+50608 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0018442:-50557 Msa1365960:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 tig0018442:+50397 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0018442:+50518 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0018442:-50655 Msa1365960:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0018442:-50493 Msa1365960:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 tig0018442:-50407 Msa1365960:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0018442:-50464 Msa1365960:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0018442:-50369 Msa1365960:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0018442:+50517 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 tig0018442:-50412 Msa1365960:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 tig0018442:-50413 Msa1365960:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0018442:-50363 Msa1365960:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0018442:+50446 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0018442:+50481 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0018442:-50701 Msa1365960:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 tig0018442:-50597 Msa1365960:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0018442:-50515 Msa1365960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + tig0018442:50348-50367 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - tig0018442:50340-50359 Msa1365960:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - tig0018442:50354-50373 Msa1365960:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - tig0018442:50386-50405 Msa1365960:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + tig0018442:50476-50495 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - tig0018442:50615-50634 Msa1365960:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + tig0018442:50443-50462 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - tig0018442:50443-50462 Msa1365960:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + tig0018442:50475-50494 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + tig0018442:50527-50546 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - tig0018442:50672-50691 Msa1365960:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - tig0018442:50442-50461 Msa1365960:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - tig0018442:50444-50463 Msa1365960:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - tig0018442:50484-50503 Msa1365960:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - tig0018442:50514-50533 Msa1365960:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + tig0018442:50526-50545 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - tig0018442:50526-50545 Msa1365960:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - tig0018442:50556-50575 Msa1365960:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + tig0018442:50588-50607 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - tig0018442:50588-50607 Msa1365960:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + tig0018442:50598-50617 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + tig0018442:50629-50648 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - tig0018442:50629-50648 Msa1365960:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + tig0018442:50665-50684 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + tig0018442:50436-50455 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - tig0018442:50548-50567 Msa1365960:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + tig0018442:50563-50582 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + tig0018442:50587-50606 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - tig0018442:50628-50647 Msa1365960:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - tig0018442:50678-50697 Msa1365960:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + tig0018442:50542-50561 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + tig0018442:50647-50666 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - tig0018442:50654-50673 Msa1365960:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - tig0018442:50634-50653 Msa1365960:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - tig0018442:50577-50596 Msa1365960:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018442 gene 50334 50729 50334 ID=Msa1365960;Name=Msa1365960
tig0018442 mRNA 50334 50729 50334 ID=Msa1365960-mRNA-1;Parent=Msa1365960;Name=Msa1365960-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
tig0018442 exon 50334 50729 50334 ID=Msa1365960-mRNA-1:exon:14880;Parent=Msa1365960-mRNA-1
tig0018442 CDS 50334 50729 50334 ID=Msa1365960-mRNA-1:cds;Parent=Msa1365960-mRNA-1
Gene Sequence

>Msa1365960

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1365960

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*