AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1391100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391100 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391100 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391100 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391100 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa1391100 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391100 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1391100 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1391100 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa1391100 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0021410:-24078 Msa1391100:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0021410:-24120 Msa1391100:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0021410:-24046 Msa1391100:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0021410:-24019 Msa1391100:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0021410:-23980 Msa1391100:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 tig0021410:+24008 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0021410:+24194 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0021410:+24201 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0021410:-24150 Msa1391100:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 tig0021410:+23990 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0021410:+24111 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0021410:-24248 Msa1391100:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0021410:-24086 Msa1391100:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 tig0021410:-24000 Msa1391100:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0021410:-24057 Msa1391100:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0021410:-23962 Msa1391100:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0021410:+24110 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 tig0021410:-24005 Msa1391100:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 tig0021410:-24006 Msa1391100:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0021410:-23956 Msa1391100:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0021410:+24039 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0021410:+24074 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0021410:-24294 Msa1391100:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 tig0021410:-24190 Msa1391100:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0021410:-24108 Msa1391100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + tig0021410:23941-23960 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - tig0021410:23933-23952 Msa1391100:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - tig0021410:23947-23966 Msa1391100:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - tig0021410:23979-23998 Msa1391100:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + tig0021410:24069-24088 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - tig0021410:24208-24227 Msa1391100:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + tig0021410:24036-24055 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - tig0021410:24036-24055 Msa1391100:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + tig0021410:24068-24087 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + tig0021410:24120-24139 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - tig0021410:24265-24284 Msa1391100:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - tig0021410:24035-24054 Msa1391100:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - tig0021410:24037-24056 Msa1391100:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - tig0021410:24077-24096 Msa1391100:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - tig0021410:24107-24126 Msa1391100:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + tig0021410:24119-24138 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - tig0021410:24119-24138 Msa1391100:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - tig0021410:24149-24168 Msa1391100:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + tig0021410:24181-24200 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - tig0021410:24181-24200 Msa1391100:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + tig0021410:24191-24210 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + tig0021410:24222-24241 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - tig0021410:24222-24241 Msa1391100:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + tig0021410:24258-24277 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + tig0021410:24029-24048 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - tig0021410:24141-24160 Msa1391100:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + tig0021410:24156-24175 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + tig0021410:24180-24199 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - tig0021410:24221-24240 Msa1391100:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - tig0021410:24271-24290 Msa1391100:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + tig0021410:24135-24154 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + tig0021410:24240-24259 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - tig0021410:24247-24266 Msa1391100:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - tig0021410:24227-24246 Msa1391100:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - tig0021410:24170-24189 Msa1391100:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0021410 gene 23927 24322 23927 ID=Msa1391100;Name=Msa1391100
tig0021410 mRNA 23927 24322 23927 ID=Msa1391100-mRNA-1;Parent=Msa1391100;Name=Msa1391100-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
tig0021410 exon 23927 24322 23927 ID=Msa1391100-mRNA-1:exon:12542;Parent=Msa1391100-mRNA-1
tig0021410 CDS 23927 24322 23927 ID=Msa1391100-mRNA-1:cds;Parent=Msa1391100-mRNA-1
Gene Sequence

>Msa1391100

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1391100

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*