AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0924930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924930 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0924930 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0924930 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0924930 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa0924930 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924930 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0924930 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0924930 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa0924930 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 6_2:+67486452 Msa0924930:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 6_2:+67486410 Msa0924930:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 6_2:+67486484 Msa0924930:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 6_2:+67486511 Msa0924930:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 6_2:+67486550 Msa0924930:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 6_2:-67486522 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 6_2:-67486336 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 6_2:-67486329 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 6_2:+67486380 Msa0924930:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 6_2:-67486540 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 6_2:-67486419 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 6_2:+67486282 Msa0924930:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 6_2:+67486444 Msa0924930:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 6_2:+67486530 Msa0924930:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 6_2:+67486473 Msa0924930:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 6_2:+67486568 Msa0924930:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 6_2:-67486420 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 6_2:+67486525 Msa0924930:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 6_2:+67486524 Msa0924930:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 6_2:+67486574 Msa0924930:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 6_2:-67486491 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 6_2:-67486456 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 6_2:+67486236 Msa0924930:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 6_2:+67486340 Msa0924930:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 6_2:+67486422 Msa0924930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG - chr6_2:67486244-67486263 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG + chr6_2:67486236-67486255 Msa0924930:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG + chr6_2:67486250-67486269 Msa0924930:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG + chr6_2:67486282-67486301 Msa0924930:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG - chr6_2:67486372-67486391 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG + chr6_2:67486511-67486530 Msa0924930:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG - chr6_2:67486339-67486358 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG + chr6_2:67486339-67486358 Msa0924930:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG - chr6_2:67486371-67486390 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG - chr6_2:67486423-67486442 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG + chr6_2:67486568-67486587 Msa0924930:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG + chr6_2:67486338-67486357 Msa0924930:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG + chr6_2:67486340-67486359 Msa0924930:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG + chr6_2:67486380-67486399 Msa0924930:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG + chr6_2:67486410-67486429 Msa0924930:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG - chr6_2:67486422-67486441 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG + chr6_2:67486422-67486441 Msa0924930:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG + chr6_2:67486452-67486471 Msa0924930:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG - chr6_2:67486484-67486503 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG + chr6_2:67486484-67486503 Msa0924930:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG - chr6_2:67486494-67486513 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG - chr6_2:67486525-67486544 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG + chr6_2:67486525-67486544 Msa0924930:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG - chr6_2:67486561-67486580 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG - chr6_2:67486332-67486351 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG + chr6_2:67486444-67486463 Msa0924930:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG - chr6_2:67486459-67486478 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG - chr6_2:67486483-67486502 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG + chr6_2:67486524-67486543 Msa0924930:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG + chr6_2:67486574-67486593 Msa0924930:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG - chr6_2:67486438-67486457 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG - chr6_2:67486543-67486562 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG + chr6_2:67486550-67486569 Msa0924930:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG + chr6_2:67486530-67486549 Msa0924930:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG + chr6_2:67486473-67486492 Msa0924930:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 67486230 67486625 67486230 ID=Msa0924930;Name=Msa0924930
chr6_2 mRNA 67486230 67486625 67486230 ID=Msa0924930-mRNA-1;Parent=Msa0924930;Name=Msa0924930-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
chr6_2 exon 67486230 67486625 67486230 ID=Msa0924930-mRNA-1:exon:10635;Parent=Msa0924930-mRNA-1
chr6_2 CDS 67486230 67486625 67486230 ID=Msa0924930-mRNA-1:cds;Parent=Msa0924930-mRNA-1
Gene Sequence

>Msa0924930

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa0924930

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*