AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354690 MtrunA17_CPg0492421 75.472 159 26 2 1 149 1 156 3.10e-76 226
Msa1354690 MtrunA17_Chr4g0016101 75.472 159 26 2 1 149 1 156 3.10e-76 226
Msa1354690 MtrunA17_Chr3g0090841 75.472 159 26 2 1 149 1 156 3.10e-76 226
Msa1354690 MtrunA17_Chr4g0002541 73.333 60 8 1 25 76 1 60 8.82e-22 84.3
Msa1354690 MtrunA17_CPg0492431 97.297 37 1 0 165 201 31 67 2.47e-17 78.2
Msa1354690 MtrunA17_Chr3g0090851 97.297 37 1 0 165 201 31 67 2.47e-17 78.2
Msa1354690 MtrunA17_Chr4g0016091 97.297 37 1 0 165 201 31 67 2.47e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354690 ATCG00900.1 77.070 157 25 2 1 149 1 154 1.86e-79 234
Msa1354690 ATCG01240.1 77.070 157 25 2 1 149 1 154 1.86e-79 234

Find 34 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAATCGATTACGATAAAT+TGG 0.238141 tig0014543:-451850 None:intergenic
TCCGGAATTGATCCTAATTT+TGG 0.252033 tig0014543:+452596 Msa1354690:CDS
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0014543:+452099 Msa1354690:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0014543:+452057 Msa1354690:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0014543:+452131 Msa1354690:CDS
AGAAGAATTAGACCAAAATT+AGG 0.319968 tig0014543:-452608 None:intergenic
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0014543:+452158 Msa1354690:CDS
ACCAAAATTAGGATCAATTC+CGG 0.350320 tig0014543:-452597 None:intergenic
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0014543:+452196 Msa1354690:CDS
CTTGGCAGCATCCACTAATT+CGG 0.392237 tig0014543:-452169 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0014543:-451983 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0014543:-451976 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0014543:+452027 Msa1354690:intron
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0014543:-452066 None:intergenic
CGAATTAGTGGATGCTGCCA+AGG 0.489621 tig0014543:+452170 Msa1354690:CDS
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0014543:+451929 Msa1354690:CDS
GTATGGCATCGCCATTCCCT+TGG 0.512820 tig0014543:-452187 None:intergenic
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0014543:+452091 Msa1354690:CDS
TAATCGATTAGTTAACATGT+TGG 0.538486 tig0014543:+451861 Msa1354690:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0014543:+452120 Msa1354690:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0014543:+452214 Msa1354690:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0014543:-452067 None:intergenic
AGTGGATGCTGCCAAGGGAA+TGG 0.548588 tig0014543:+452176 Msa1354690:CDS
TCATGGGTTCTTCTCTCCAT+CGG 0.567612 tig0014543:-452727 None:intergenic
TAACGGCGCTGTTGTTCCGA+TGG 0.571426 tig0014543:+452711 Msa1354690:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0014543:+452220 Msa1354690:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0014543:-452138 None:intergenic
GAATTAGTGGATGCTGCCAA+GGG 0.619190 tig0014543:+452171 Msa1354690:CDS
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0014543:-452103 None:intergenic
TCGATTACGATAAATTGGAT+CGG 0.627987 tig0014543:-451845 None:intergenic
AAGTTTAGTAATGAGCATAA+CGG 0.644237 tig0014543:+452694 Msa1354690:CDS
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0014543:+451883 Msa1354690:CDS
TTTACGCTCATGTCACGGCG+AGG 0.672550 tig0014543:+451796 None:intergenic
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0014543:+452069 Msa1354690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAGAGGAATATT+TGG + tig0014543:452441-452460 Msa1354690:intron 15.0%
!! AATAAATAAAGAGGAATATT+TGG + tig0014543:452441-452460 Msa1354690:intron 15.0%
!! AGAATCAATAGAAAAAGAAT+CGG + tig0014543:452338-452357 Msa1354690:intron 20.0%
!! TTGCTTAAGAATAAATAAAG+AGG + tig0014543:452432-452451 Msa1354690:intron 20.0%
!!! AGGAATATTTGGTATTTTTT+TGG + tig0014543:452452-452471 Msa1354690:intron 20.0%
!! AGAATCAATAGAAAAAGAAT+CGG + tig0014543:452338-452357 Msa1354690:intron 20.0%
!! TTGCTTAAGAATAAATAAAG+AGG + tig0014543:452432-452451 Msa1354690:intron 20.0%
!!! AGGAATATTTGGTATTTTTT+TGG + tig0014543:452452-452471 Msa1354690:intron 20.0%
! ACTAATCGATTACGATAAAT+TGG - tig0014543:451853-451872 None:intergenic 25.0%
!! TAATCGATTAGTTAACATGT+TGG + tig0014543:451861-451880 Msa1354690:CDS 25.0%
!! TTTTTCCGTGTTTCATAATA+CGG - tig0014543:451891-451910 None:intergenic 25.0%
!! GATTCTTTTCCAATTATGTA+TGG - tig0014543:452324-452343 None:intergenic 25.0%
! AGAAGAATTAGACCAAAATT+AGG - tig0014543:452611-452630 None:intergenic 25.0%
! AAGTTTAGTAATGAGCATAA+CGG + tig0014543:452694-452713 Msa1354690:CDS 25.0%
! ACTAATCGATTACGATAAAT+TGG - tig0014543:451853-451872 None:intergenic 25.0%
!! TAATCGATTAGTTAACATGT+TGG + tig0014543:451861-451880 Msa1354690:CDS 25.0%
!! TTTTTCCGTGTTTCATAATA+CGG - tig0014543:451891-451910 None:intergenic 25.0%
!! GATTCTTTTCCAATTATGTA+TGG - tig0014543:452324-452343 None:intergenic 25.0%
! AGAAGAATTAGACCAAAATT+AGG - tig0014543:452611-452630 None:intergenic 25.0%
! AAGTTTAGTAATGAGCATAA+CGG + tig0014543:452694-452713 Msa1354690:CDS 25.0%
! ATAAATTGGATCGGATTTTG+CGG - tig0014543:451839-451858 None:intergenic 30.0%
TCGATTACGATAAATTGGAT+CGG - tig0014543:451848-451867 None:intergenic 30.0%
GTTAACCGTATTATGAAACA+CGG + tig0014543:451883-451902 Msa1354690:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG + tig0014543:451897-451916 Msa1354690:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG + tig0014543:451929-451948 Msa1354690:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG - tig0014543:452019-452038 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG + tig0014543:452158-452177 Msa1354690:CDS 30.0%
ACATAGACCTATGTTATGTA+TGG + tig0014543:452296-452315 Msa1354690:intron 30.0%
TATGGATCCATACATAACAT+AGG - tig0014543:452306-452325 None:intergenic 30.0%
TGTATGGATCCATACATAAT+TGG + tig0014543:452312-452331 Msa1354690:intron 30.0%
GAAAGACGAAATAGAAATGA+TGG + tig0014543:452386-452405 Msa1354690:intron 30.0%
ATCGAAAAGAAGCAAATGAA+AGG - tig0014543:452562-452581 None:intergenic 30.0%
! TTTCATTTGCTTCTTTTCGA+TGG + tig0014543:452561-452580 Msa1354690:intron 30.0%
!!! CGATGGAAGTTTTATTTTTC+CGG + tig0014543:452578-452597 Msa1354690:intron 30.0%
ACCAAAATTAGGATCAATTC+CGG - tig0014543:452600-452619 None:intergenic 30.0%
! AGATAGATCTTTTTGATCAG+AGG - tig0014543:452651-452670 None:intergenic 30.0%
CTGATCAAAAAGATCTATCT+TGG + tig0014543:452651-452670 Msa1354690:CDS 30.0%
! ATAAATTGGATCGGATTTTG+CGG - tig0014543:451839-451858 None:intergenic 30.0%
TCGATTACGATAAATTGGAT+CGG - tig0014543:451848-451867 None:intergenic 30.0%
GTTAACCGTATTATGAAACA+CGG + tig0014543:451883-451902 Msa1354690:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG + tig0014543:451897-451916 Msa1354690:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG + tig0014543:451929-451948 Msa1354690:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG - tig0014543:452019-452038 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG + tig0014543:452158-452177 Msa1354690:CDS 30.0%
ACATAGACCTATGTTATGTA+TGG + tig0014543:452296-452315 Msa1354690:intron 30.0%
TATGGATCCATACATAACAT+AGG - tig0014543:452306-452325 None:intergenic 30.0%
TGTATGGATCCATACATAAT+TGG + tig0014543:452312-452331 Msa1354690:intron 30.0%
GAAAGACGAAATAGAAATGA+TGG + tig0014543:452386-452405 Msa1354690:intron 30.0%
ATCGAAAAGAAGCAAATGAA+AGG - tig0014543:452562-452581 None:intergenic 30.0%
! TTTCATTTGCTTCTTTTCGA+TGG + tig0014543:452561-452580 Msa1354690:intron 30.0%
!!! CGATGGAAGTTTTATTTTTC+CGG + tig0014543:452578-452597 Msa1354690:intron 30.0%
ACCAAAATTAGGATCAATTC+CGG - tig0014543:452600-452619 None:intergenic 30.0%
! AGATAGATCTTTTTGATCAG+AGG - tig0014543:452651-452670 None:intergenic 30.0%
CTGATCAAAAAGATCTATCT+TGG + tig0014543:452651-452670 Msa1354690:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG - tig0014543:451986-452005 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG + tig0014543:451986-452005 Msa1354690:intron 35.0%
!! CTTGCTTTTACTGCTATATC+GGG - tig0014543:452018-452037 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG - tig0014543:452070-452089 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG + tig0014543:452214-452233 Msa1354690:CDS 35.0%
! ATTTTCGTTAATCCATGAGC+AGG + tig0014543:452261-452280 Msa1354690:intron 35.0%
! TCCGGAATTGATCCTAATTT+TGG + tig0014543:452596-452615 Msa1354690:CDS 35.0%
ACGTATTGCTTGACGTAAAA+CGG - tig0014543:451986-452005 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG + tig0014543:451986-452005 Msa1354690:intron 35.0%
!! CTTGCTTTTACTGCTATATC+GGG - tig0014543:452018-452037 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG - tig0014543:452070-452089 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG + tig0014543:452214-452233 Msa1354690:CDS 35.0%
! ATTTTCGTTAATCCATGAGC+AGG + tig0014543:452261-452280 Msa1354690:intron 35.0%
! TCCGGAATTGATCCTAATTT+TGG + tig0014543:452596-452615 Msa1354690:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG + tig0014543:451985-452004 Msa1354690:intron 40.0%
TTTACGTCAAGCAATACGTG+GGG + tig0014543:451987-452006 Msa1354690:intron 40.0%
GTAAAAGCAAGACGCGTAAA+CGG + tig0014543:452027-452046 Msa1354690:intron 40.0%
CATCAAGTTCCCGTTGAAAT+AGG + tig0014543:452057-452076 Msa1354690:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG - tig0014543:452069-452088 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG + tig0014543:452069-452088 Msa1354690:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG + tig0014543:452099-452118 Msa1354690:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG - tig0014543:452131-452150 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG + tig0014543:452131-452150 Msa1354690:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG - tig0014543:452141-452160 None:intergenic 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG - tig0014543:452207-452226 None:intergenic 40.0%
GTGTATATAGATCCTGCTCA+TGG - tig0014543:452276-452295 None:intergenic 40.0%
! ATGGCTCAACTAAGCACTTT+TGG + tig0014543:452405-452424 Msa1354690:intron 40.0%
! TGGCTCAACTAAGCACTTTT+GGG + tig0014543:452406-452425 Msa1354690:intron 40.0%
! GTTTTACGTCAAGCAATACG+TGG + tig0014543:451985-452004 Msa1354690:intron 40.0%
TTTACGTCAAGCAATACGTG+GGG + tig0014543:451987-452006 Msa1354690:intron 40.0%
GTAAAAGCAAGACGCGTAAA+CGG + tig0014543:452027-452046 Msa1354690:intron 40.0%
CATCAAGTTCCCGTTGAAAT+AGG + tig0014543:452057-452076 Msa1354690:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG - tig0014543:452069-452088 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG + tig0014543:452069-452088 Msa1354690:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG + tig0014543:452099-452118 Msa1354690:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG - tig0014543:452131-452150 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG + tig0014543:452131-452150 Msa1354690:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG - tig0014543:452141-452160 None:intergenic 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG - tig0014543:452207-452226 None:intergenic 40.0%
GTGTATATAGATCCTGCTCA+TGG - tig0014543:452276-452295 None:intergenic 40.0%
! ATGGCTCAACTAAGCACTTT+TGG + tig0014543:452405-452424 Msa1354690:intron 40.0%
! TGGCTCAACTAAGCACTTTT+GGG + tig0014543:452406-452425 Msa1354690:intron 40.0%
GCTTGACGTAAAACGGATAG+TGG - tig0014543:451979-451998 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG + tig0014543:452091-452110 Msa1354690:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG - tig0014543:452106-452125 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG - tig0014543:452130-452149 None:intergenic 45.0%
CTTGGCAGCATCCACTAATT+CGG - tig0014543:452172-452191 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+GGG + tig0014543:452171-452190 Msa1354690:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG + tig0014543:452220-452239 Msa1354690:CDS 45.0%
! GGCTCAACTAAGCACTTTTG+GGG + tig0014543:452407-452426 Msa1354690:intron 45.0%
TCATGGGTTCTTCTCTCCAT+CGG - tig0014543:452730-452749 None:intergenic 45.0%
GCTTGACGTAAAACGGATAG+TGG - tig0014543:451979-451998 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG + tig0014543:452091-452110 Msa1354690:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG - tig0014543:452106-452125 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG - tig0014543:452130-452149 None:intergenic 45.0%
CTTGGCAGCATCCACTAATT+CGG - tig0014543:452172-452191 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+GGG + tig0014543:452171-452190 Msa1354690:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG + tig0014543:452220-452239 Msa1354690:CDS 45.0%
! GGCTCAACTAAGCACTTTTG+GGG + tig0014543:452407-452426 Msa1354690:intron 45.0%
TCATGGGTTCTTCTCTCCAT+CGG - tig0014543:452730-452749 None:intergenic 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG - tig0014543:452085-452104 None:intergenic 50.0%
! CGAATTAGTGGATGCTGCCA+AGG + tig0014543:452170-452189 Msa1354690:CDS 50.0%
TGGCGATGCCATACGCAAAA+AGG + tig0014543:452196-452215 Msa1354690:CDS 50.0%
! GGCAAGTGCTTTTCCTTGTG+TGG - tig0014543:452085-452104 None:intergenic 50.0%
! CGAATTAGTGGATGCTGCCA+AGG + tig0014543:452170-452189 Msa1354690:CDS 50.0%
TGGCGATGCCATACGCAAAA+AGG + tig0014543:452196-452215 Msa1354690:CDS 50.0%
AGTGGATGCTGCCAAGGGAA+TGG + tig0014543:452176-452195 Msa1354690:CDS 55.0%
GTATGGCATCGCCATTCCCT+TGG - tig0014543:452190-452209 None:intergenic 55.0%
TAACGGCGCTGTTGTTCCGA+TGG + tig0014543:452711-452730 Msa1354690:CDS 55.0%
AGTGGATGCTGCCAAGGGAA+TGG + tig0014543:452176-452195 Msa1354690:CDS 55.0%
GTATGGCATCGCCATTCCCT+TGG - tig0014543:452190-452209 None:intergenic 55.0%
TAACGGCGCTGTTGTTCCGA+TGG + tig0014543:452711-452730 Msa1354690:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG + tig0014543:452120-452139 Msa1354690:CDS 65.0%
GGCGCATCCCGAAAACGTCC+AGG + tig0014543:452120-452139 Msa1354690:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 451805 452751 451805 ID=Msa1354690;Name=Msa1354690
tig0014543 mRNA 451805 452751 451805 ID=Msa1354690-mRNA-1;Parent=Msa1354690;Name=Msa1354690-mRNA-1;_AED=0.36;_eAED=0.42;_QI=0|0|0|1|0|0|3|0|201
tig0014543 exon 451805 452003 451805 ID=Msa1354690-mRNA-1:exon:9202;Parent=Msa1354690-mRNA-1
tig0014543 exon 452028 452266 452028 ID=Msa1354690-mRNA-1:exon:9203;Parent=Msa1354690-mRNA-1
tig0014543 exon 452587 452751 452587 ID=Msa1354690-mRNA-1:exon:9204;Parent=Msa1354690-mRNA-1
tig0014543 CDS 451805 452003 451805 ID=Msa1354690-mRNA-1:cds;Parent=Msa1354690-mRNA-1
tig0014543 CDS 452028 452266 452028 ID=Msa1354690-mRNA-1:cds;Parent=Msa1354690-mRNA-1
tig0014543 CDS 452587 452751 452587 ID=Msa1354690-mRNA-1:cds;Parent=Msa1354690-mRNA-1
Gene Sequence

>Msa1354690

ATGTCACGGCGAGGTACTGCAGAAAAAAAAACCGCAAAATCCGATCCAATTTATCGTAATCGATTAGTTAACATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCTTTTATTTTTCCGGAATTGATCCTAATTTTGGTCTAATTCTTCTTCTGATGATTGATTTAACCTCTGATCAAAAAGATCTATCTTGGTTCTATTTTATCTCTTCAACAAGTTTAGTAATGAGCATAACGGCGCTGTTGTTCCGATGGAGAGAAGAACCCATGATT

Protein sequence

>Msa1354690

MSRRGTAEKKTAKSDPIYRNRLVNMLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAILKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGMAMPYAKRKRLIEWQRQIELLHIFFYFSGIDPNFGLILLLMIDLTSDQKDLSWFYFISSTSLVMSITALLFRWREEPMI