AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0269140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0269140 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0269140 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0269140 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0269140 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa0269140 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0269140 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0269140 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0269140 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa0269140 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 2_2:-70536026 Msa0269140:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 2_2:-70536068 Msa0269140:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 2_2:-70535994 Msa0269140:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 2_2:-70535967 Msa0269140:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 2_2:-70535928 Msa0269140:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 2_2:+70535956 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 2_2:+70536142 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 2_2:+70536149 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 2_2:-70536098 Msa0269140:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 2_2:+70535938 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 2_2:+70536059 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 2_2:-70536196 Msa0269140:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 2_2:-70536034 Msa0269140:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 2_2:-70535948 Msa0269140:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 2_2:-70536005 Msa0269140:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 2_2:-70535910 Msa0269140:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 2_2:+70536058 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 2_2:-70535953 Msa0269140:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 2_2:-70535954 Msa0269140:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 2_2:-70535904 Msa0269140:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 2_2:+70535987 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 2_2:+70536022 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 2_2:-70536242 Msa0269140:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 2_2:-70536138 Msa0269140:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 2_2:-70536056 Msa0269140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + chr2_2:70535889-70535908 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - chr2_2:70535881-70535900 Msa0269140:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - chr2_2:70535895-70535914 Msa0269140:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - chr2_2:70535927-70535946 Msa0269140:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + chr2_2:70536017-70536036 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - chr2_2:70536156-70536175 Msa0269140:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + chr2_2:70535984-70536003 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - chr2_2:70535984-70536003 Msa0269140:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + chr2_2:70536016-70536035 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + chr2_2:70536068-70536087 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - chr2_2:70536213-70536232 Msa0269140:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - chr2_2:70535983-70536002 Msa0269140:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - chr2_2:70535985-70536004 Msa0269140:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - chr2_2:70536025-70536044 Msa0269140:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - chr2_2:70536055-70536074 Msa0269140:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + chr2_2:70536067-70536086 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - chr2_2:70536067-70536086 Msa0269140:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - chr2_2:70536097-70536116 Msa0269140:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + chr2_2:70536129-70536148 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - chr2_2:70536129-70536148 Msa0269140:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + chr2_2:70536139-70536158 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + chr2_2:70536170-70536189 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - chr2_2:70536170-70536189 Msa0269140:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + chr2_2:70536206-70536225 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + chr2_2:70535977-70535996 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - chr2_2:70536089-70536108 Msa0269140:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + chr2_2:70536104-70536123 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + chr2_2:70536128-70536147 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - chr2_2:70536169-70536188 Msa0269140:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - chr2_2:70536219-70536238 Msa0269140:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + chr2_2:70536083-70536102 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + chr2_2:70536188-70536207 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - chr2_2:70536195-70536214 Msa0269140:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - chr2_2:70536175-70536194 Msa0269140:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - chr2_2:70536118-70536137 Msa0269140:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70535875 70536270 70535875 ID=Msa0269140;Name=Msa0269140
chr2_2 mRNA 70535875 70536270 70535875 ID=Msa0269140-mRNA-1;Parent=Msa0269140;Name=Msa0269140-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
chr2_2 exon 70535875 70536270 70535875 ID=Msa0269140-mRNA-1:exon:22739;Parent=Msa0269140-mRNA-1
chr2_2 CDS 70535875 70536270 70535875 ID=Msa0269140-mRNA-1:cds;Parent=Msa0269140-mRNA-1
Gene Sequence

>Msa0269140

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa0269140

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*