AlfalfaGEDB | Msa1086730

M. sativa cultivar zhongmu-4 / Msa1086730

KEGG: K02992
GO: GO:0000028 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00177.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086730	YP_009141653.1	100.000	131	0	0	1	131	25	155	1.21e-90	269

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086730	sp\|P07135\|RR7_SOYBN	96.947	131	4	0	1	131	25	155	1.13e-90	262

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086730	A0A0F6NLW0	100.000	131	0	0	1	131	25	155	5.78e-91	269

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095190	Msa1086730	0.817413	3.341181e-52	-8.615850e-47
Msa0139800	Msa1086730	0.817795	2.739007e-52	-8.615850e-47
Msa0202640	Msa1086730	0.837036	6.586037e-57	-8.615850e-47
Msa0370210	Msa1086730	0.825581	4.335048e-54	-8.615850e-47
Msa0398630	Msa1086730	0.803233	3.844019e-49	-8.615850e-47
Msa0405420	Msa1086730	0.829106	6.195643e-55	-8.615850e-47
Msa0405430	Msa1086730	0.854046	1.597340e-61	-8.615850e-47
Msa0405440	Msa1086730	0.849973	2.292532e-60	-8.615850e-47
Msa0405510	Msa1086730	0.840916	6.517029e-58	-8.615850e-47
Msa0405520	Msa1086730	0.843638	1.238855e-58	-8.615850e-47
Msa0405530	Msa1086730	0.863757	1.981747e-64	-8.615850e-47
Msa0983630	Msa1086730	0.907772	3.530984e-81	-8.615850e-47
Msa0983640	Msa1086730	0.854403	1.260071e-61	-8.615850e-47
Msa0983720	Msa1086730	0.801465	8.888338e-49	-8.615850e-47
Msa1022050	Msa1086730	0.895917	6.071083e-76	-8.615850e-47
Msa1022060	Msa1086730	0.835369	1.747111e-56	-8.615850e-47
Msa1064410	Msa1086730	0.854741	1.005958e-61	-8.615850e-47
Msa1086070	Msa1086730	0.803625	3.188541e-49	-8.615850e-47
Msa1086080	Msa1086730	0.819196	1.318350e-52	-8.615850e-47
Msa1086090	Msa1086730	0.838868	2.226486e-57	-8.615850e-47
Msa1086100	Msa1086730	0.830857	2.318941e-55	-8.615850e-47
Msa1086140	Msa1086730	0.850956	1.214447e-60	-8.615850e-47
Msa1086150	Msa1086730	0.807353	5.268738e-50	-8.615850e-47
Msa1086180	Msa1086730	0.804562	2.035751e-49	-8.615850e-47
Msa1086190	Msa1086730	0.849055	4.133500e-60	-8.615850e-47
Msa1086200	Msa1086730	0.839500	1.527265e-57	-8.615850e-47
Msa1086210	Msa1086730	0.824475	7.910565e-54	-8.615850e-47
Msa1086250	Msa1086730	0.816564	5.181287e-52	-8.615850e-47
Msa1086270	Msa1086730	0.850016	2.230238e-60	-8.615850e-47
Msa1086280	Msa1086730	0.805132	1.546838e-49	-8.615850e-47
Msa1086380	Msa1086730	0.822093	2.848286e-53	-8.615850e-47
Msa1086470	Msa1086730	0.802240	6.162625e-49	-8.615850e-47
Msa1086550	Msa1086730	0.845306	4.410739e-59	-8.615850e-47
Msa1086560	Msa1086730	0.845405	4.146355e-59	-8.615850e-47
Msa1086590	Msa1086730	0.873917	1.014017e-67	-8.615850e-47
Msa1086600	Msa1086730	0.869523	2.906174e-66	-8.615850e-47
Msa1086720	Msa1086730	0.871038	9.260990e-67	-8.615850e-47
Msa1086730	Msa1086740	0.881079	3.232619e-70	-8.615850e-47
Msa1086730	Msa1086790	0.910525	1.698984e-82	-8.615850e-47
Msa1086730	Msa1086800	0.881810	1.761657e-70	-8.615850e-47
Msa1086730	Msa1086860	0.804525	2.071753e-49	-8.615850e-47
Msa1086730	Msa1086880	0.813927	1.998128e-51	-8.615850e-47
Msa1086730	Msa1086890	0.838765	2.367333e-57	-8.615850e-47
Msa1086730	Msa1086900	0.833903	4.080460e-56	-8.615850e-47
Msa1086730	Msa1086910	0.829812	4.174449e-55	-8.615850e-47
Msa1086730	Msa1086960	0.899639	1.624554e-77	-8.615850e-47
Msa1086730	Msa1086970	0.844500	7.276495e-59	-8.615850e-47
Msa1086730	Msa1087030	0.860295	2.281345e-63	-8.615850e-47
Msa1086730	Msa1087040	0.933527	1.639538e-95	-8.615850e-47
Msa1086730	Msa1087050	0.873554	1.343857e-67	-8.615850e-47
Msa1086730	Msa1087110	0.913350	6.810900e-84	-8.615850e-47
Msa1086730	Msa1087120	0.868462	6.412067e-66	-8.615850e-47
Msa1086730	Msa1143120	0.859496	3.973062e-63	-8.615850e-47
Msa1086730	Msa1143130	0.819447	1.156192e-52	-8.615850e-47
Msa1086730	Msa1143160	0.839887	1.210966e-57	-8.615850e-47
Msa1086730	Msa1143170	0.835317	1.800276e-56	-8.615850e-47
Msa1086730	Msa1143180	0.819768	9.762465e-53	-8.615850e-47
Msa1086730	Msa1305090	0.847962	8.296754e-60	-8.615850e-47
Msa1086730	Msa1354320	0.804431	2.167636e-49	-8.615850e-47
Msa1086730	Msa1354420	0.903271	4.122522e-79	-8.615850e-47
Msa1086730	Msa1354430	0.845649	3.559533e-59	-8.615850e-47
Msa1086730	Msa1354480	0.856513	3.057328e-62	-8.615850e-47
Msa1086730	Msa1354490	0.923576	2.213428e-89	-8.615850e-47
Msa1086730	Msa1354500	0.882076	1.411027e-70	-8.615850e-47
Msa1086730	Msa1354550	0.911323	6.926147e-83	-8.615850e-47
Msa1086730	Msa1354560	0.839911	1.193236e-57	-8.615850e-47
Msa1086730	Msa1354610	0.879330	1.361337e-69	-8.615850e-47
Msa1086730	Msa1354620	0.909842	3.638154e-82	-8.615850e-47
Msa1086730	Msa1354630	0.885976	5.108793e-72	-8.615850e-47
Msa1086730	Msa1354680	0.853793	1.888636e-61	-8.615850e-47
Msa1086730	Msa1354690	0.902451	9.565794e-79	-8.615850e-47
Msa1086730	Msa1354700	0.836877	7.233907e-57	-8.615850e-47
Msa1086730	Msa1354740	0.868252	7.496058e-66	-8.615850e-47
Msa1086730	Msa1354750	0.915838	3.656992e-85	-8.615850e-47
Msa1086730	Msa1354760	0.872816	2.377214e-67	-8.615850e-47
Msa1086730	Msa1354810	0.914677	1.447849e-84	-8.615850e-47
Msa1086730	Msa1354820	0.860445	2.054879e-63	-8.615850e-47
Msa1086730	Msa1354900	0.801599	8.345005e-49	-8.615850e-47
Msa1086730	Msa1354910	0.849329	3.467435e-60	-8.615850e-47
Msa1086730	Msa1354920	0.845325	4.356823e-59	-8.615850e-47
Msa1086730	Msa1354930	0.831265	1.840631e-55	-8.615850e-47
Msa1086730	Msa1354980	0.907704	3.798187e-81	-8.615850e-47
Msa1086730	Msa1354990	0.840447	8.648208e-58	-8.615850e-47
Msa1086730	Msa1355070	0.810872	9.286530e-51	-8.615850e-47
Msa1086730	Msa1355080	0.843863	1.078375e-58	-8.615850e-47
Msa1086730	Msa1355090	0.845069	5.109546e-59	-8.615850e-47
Msa1086730	Msa1355100	0.811996	5.294822e-51	-8.615850e-47
Msa1086730	Msa1365890	0.883067	6.136313e-71	-8.615850e-47
Msa1086730	Msa1365900	0.840942	6.416707e-58	-8.615850e-47
Msa1086730	Msa1365950	0.858284	9.156767e-63	-8.615850e-47
Msa1086730	Msa1365960	0.896800	2.603524e-76	-8.615850e-47
Msa1086730	Msa1365970	0.859400	4.244849e-63	-8.615850e-47
Msa1086730	Msa1366080	0.906583	1.270738e-80	-8.615850e-47
Msa1086730	Msa1366090	0.841110	5.794764e-58	-8.615850e-47
Msa1086730	Msa1368230	0.904353	1.341444e-79	-8.615850e-47
Msa1086730	Msa1368240	0.863552	2.294297e-64	-8.615850e-47
Msa1086730	Msa1380600	0.823103	1.658901e-53	-8.615850e-47
Msa1086730	Msa1380620	0.817597	3.035760e-52	-8.615850e-47
Msa1086730	Msa1380680	0.815183	1.053345e-51	-8.615850e-47
Msa1086730	Msa1385410	0.875022	4.274796e-68	-8.615850e-47
Msa1086730	Msa1385420	0.831328	1.776350e-55	-8.615850e-47
Msa1086730	Msa1391090	0.856470	3.147527e-62	-8.615850e-47
Msa1086730	Msa1391100	0.932031	1.566531e-94	-8.615850e-47
Msa1086730	Msa1391110	0.860287	2.294739e-63	-8.615850e-47
Msa1086730	Msa1391160	0.896174	4.750480e-76	-8.615850e-47
Msa1086730	Msa1391170	0.857088	2.070783e-62	-8.615850e-47
Msa1086730	Msa1391220	0.870077	1.916118e-66	-8.615850e-47
Msa1086730	Msa1391230	0.887323	1.578770e-72	-8.615850e-47
Msa1086730	Msa1391240	0.863136	3.087735e-64	-8.615850e-47
Msa1086730	Msa1394030	0.832912	7.212053e-56	-8.615850e-47
Msa1086730	Msa1394040	0.852160	5.537699e-61	-8.615850e-47
Msa1086730	Msa1394150	0.846200	2.521264e-59	-8.615850e-47
Msa1086730	Msa1394160	0.832001	1.212365e-55	-8.615850e-47
Msa1086730	Msa1394210	0.838784	2.340285e-57	-8.615850e-47
Msa1086730	Msa1394220	0.811985	5.322269e-51	-8.615850e-47
Msa1086730	Msa1394230	0.810167	1.319013e-50	-8.615850e-47
Msa1086730	Msa1460120	0.820911	5.339903e-53	-8.615850e-47
Msa1086730	Msa1460130	0.839970	1.151971e-57	-8.615850e-47
Msa0263770	Msa1086730	0.831727	1.417178e-55	-8.615850e-47
Msa0268170	Msa1086730	0.819729	9.968268e-53	-8.615850e-47
Msa0268250	Msa1086730	0.858619	7.275513e-63	-8.615850e-47
Msa0268260	Msa1086730	0.903757	2.492806e-79	-8.615850e-47
Msa0268270	Msa1086730	0.878613	2.437257e-69	-8.615850e-47
Msa0268300	Msa1086730	0.875725	2.457686e-68	-8.615850e-47
Msa0268310	Msa1086730	0.874518	6.345018e-68	-8.615850e-47
Msa0268390	Msa1086730	0.815143	1.075153e-51	-8.615850e-47
Msa0268400	Msa1086730	0.810179	1.310920e-50	-8.615850e-47
Msa0268410	Msa1086730	0.815604	8.490748e-52	-8.615850e-47
Msa0268550	Msa1086730	0.820985	5.134412e-53	-8.615850e-47
Msa0268560	Msa1086730	0.846534	2.045356e-59	-8.615850e-47
Msa0268570	Msa1086730	0.833308	5.744722e-56	-8.615850e-47
Msa0268580	Msa1086730	0.835766	1.385691e-56	-8.615850e-47
Msa0268630	Msa1086730	0.838754	2.383555e-57	-8.615850e-47
Msa0268640	Msa1086730	0.827629	1.407475e-54	-8.615850e-47
Msa0268740	Msa1086730	0.848745	5.039053e-60	-8.615850e-47
Msa0268750	Msa1086730	0.835440	1.676181e-56	-8.615850e-47
Msa0268760	Msa1086730	0.813294	2.752340e-51	-8.615850e-47
Msa0268830	Msa1086730	0.854139	1.501771e-61	-8.615850e-47
Msa0268840	Msa1086730	0.827177	1.807060e-54	-8.615850e-47
Msa0268940	Msa1086730	0.815286	9.992853e-52	-8.615850e-47
Msa0268950	Msa1086730	0.831599	1.523851e-55	-8.615850e-47
Msa0268960	Msa1086730	0.848500	5.893412e-60	-8.615850e-47
Msa0269040	Msa1086730	0.859781	3.261229e-63	-8.615850e-47
Msa0269050	Msa1086730	0.836268	1.033455e-56	-8.615850e-47
Msa0269130	Msa1086730	0.853235	2.731669e-61	-8.615850e-47
Msa0269140	Msa1086730	0.921127	5.324538e-88	-8.615850e-47
Msa0269150	Msa1086730	0.885253	9.531908e-72	-8.615850e-47
Msa0344180	Msa1086730	0.811388	7.178910e-51	-8.615850e-47
Msa0344190	Msa1086730	0.829369	5.349402e-55	-8.615850e-47
Msa0344300	Msa1086730	0.805540	1.270849e-49	-8.615850e-47
Msa0344310	Msa1086730	0.848559	5.672721e-60	-8.615850e-47
Msa0484040	Msa1086730	0.809258	2.067752e-50	-8.615850e-47
Msa0582510	Msa1086730	0.858386	8.537324e-63	-8.615850e-47
Msa0582520	Msa1086730	0.816465	5.454379e-52	-8.615850e-47
Msa0582630	Msa1086730	0.837568	4.814617e-57	-8.615850e-47
Msa0582640	Msa1086730	0.862122	6.334557e-64	-8.615850e-47
Msa0582650	Msa1086730	0.831093	2.029597e-55	-8.615850e-47
Msa0582660	Msa1086730	0.834573	2.771800e-56	-8.615850e-47
Msa0582690	Msa1086730	0.853356	2.522108e-61	-8.615850e-47
Msa0582700	Msa1086730	0.823066	1.692035e-53	-8.615850e-47
Msa0582750	Msa1086730	0.823831	1.120743e-53	-8.615850e-47
Msa0582760	Msa1086730	0.848468	6.011860e-60	-8.615850e-47
Msa0582770	Msa1086730	0.819652	1.037882e-52	-8.615850e-47
Msa0582780	Msa1086730	0.839133	1.900926e-57	-8.615850e-47
Msa0582810	Msa1086730	0.863130	3.101369e-64	-8.615850e-47
Msa0582820	Msa1086730	0.824067	9.866292e-54	-8.615850e-47
Msa0582910	Msa1086730	0.843289	1.535223e-58	-8.615850e-47
Msa0582920	Msa1086730	0.825578	4.343047e-54	-8.615850e-47
Msa0582930	Msa1086730	0.824858	6.426910e-54	-8.615850e-47
Msa0591290	Msa1086730	0.828526	8.558554e-55	-8.615850e-47
Msa0591310	Msa1086730	0.843896	1.056915e-58	-8.615850e-47
Msa0591350	Msa1086730	0.878288	3.171124e-69	-8.615850e-47
Msa0591360	Msa1086730	0.824280	8.794830e-54	-8.615850e-47
Msa0591380	Msa1086730	0.815149	1.071963e-51	-8.615850e-47
Msa0867680	Msa1086730	0.901481	2.565985e-78	-8.615850e-47
Msa0867690	Msa1086730	0.834558	2.795908e-56	-8.615850e-47
Msa0908220	Msa1086730	0.865070	7.710104e-65	-8.615850e-47
Msa0908230	Msa1086730	0.933856	9.908312e-96	-8.615850e-47
Msa0908240	Msa1086730	0.864971	8.277898e-65	-8.615850e-47
Msa0908310	Msa1086730	0.906981	8.296387e-81	-8.615850e-47
Msa0908320	Msa1086730	0.854073	1.568554e-61	-8.615850e-47
Msa0908370	Msa1086730	0.905833	2.824478e-80	-8.615850e-47
Msa0908380	Msa1086730	0.823829	1.122015e-53	-8.615850e-47
Msa0908430	Msa1086730	0.846626	1.929862e-59	-8.615850e-47
Msa0908440	Msa1086730	0.922000	1.735369e-88	-8.615850e-47
Msa0908450	Msa1086730	0.885866	5.619968e-72	-8.615850e-47
Msa0908480	Msa1086730	0.819611	1.060762e-52	-8.615850e-47
Msa0908530	Msa1086730	0.907133	7.043236e-81	-8.615850e-47
Msa0908540	Msa1086730	0.859731	3.375611e-63	-8.615850e-47
Msa0908590	Msa1086730	0.839060	1.985329e-57	-8.615850e-47
Msa0908600	Msa1086730	0.890876	6.623321e-74	-8.615850e-47
Msa0908610	Msa1086730	0.844367	7.901330e-59	-8.615850e-47
Msa0908650	Msa1086730	0.861909	7.364317e-64	-8.615850e-47
Msa0908660	Msa1086730	0.908571	1.477803e-81	-8.615850e-47
Msa0908670	Msa1086730	0.889201	2.992732e-73	-8.615850e-47
Msa0908720	Msa1086730	0.908923	1.004428e-81	-8.615850e-47
Msa0908730	Msa1086730	0.836335	9.940796e-57	-8.615850e-47
Msa0911190	Msa1086730	0.848821	4.800718e-60	-8.615850e-47
Msa0911200	Msa1086730	0.823228	1.550582e-53	-8.615850e-47
Msa0911230	Msa1086730	0.819321	1.235166e-52	-8.615850e-47
Msa0924830	Msa1086730	0.812792	3.547069e-51	-8.615850e-47
Msa0924870	Msa1086730	0.814235	1.708656e-51	-8.615850e-47
Msa0924880	Msa1086730	0.813193	2.897248e-51	-8.615850e-47
Msa0924890	Msa1086730	0.801892	7.267201e-49	-8.615850e-47
Msa0924930	Msa1086730	0.878333	3.057172e-69	-8.615850e-47
Msa0924940	Msa1086730	0.839540	1.491179e-57	-8.615850e-47
Msa0925040	Msa1086730	0.801469	8.871580e-49	-8.615850e-47
Msa0925100	Msa1086730	0.873791	1.118414e-67	-8.615850e-47
Msa0925110	Msa1086730	0.843735	1.166932e-58	-8.615850e-47
Msa0931070	Msa1086730	0.841447	4.726317e-58	-8.615850e-47
Msa0931080	Msa1086730	0.818908	1.533019e-52	-8.615850e-47
Msa0931130	Msa1086730	0.855224	7.281778e-62	-8.615850e-47
Msa0931140	Msa1086730	0.823202	1.572528e-53	-8.615850e-47
Msa0931220	Msa1086730	0.813798	2.133282e-51	-8.615850e-47
Msa0931230	Msa1086730	0.807946	3.942055e-50	-8.615850e-47
Msa0931240	Msa1086730	0.834647	2.655653e-56	-8.615850e-47
Msa0931260	Msa1086730	0.861007	1.387672e-63	-8.615850e-47
Msa0931270	Msa1086730	0.814267	1.680860e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086730	MtrunA17_CPg0492421	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1086730	MtrunA17_Chr4g0016101	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1086730	MtrunA17_Chr3g0090841	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1086730	MtrunA17_Chr4g0002541	83.333	60	10	0	1	60	1	60	3.60e-30	103
Msa1086730	MtrunA17_Chr4g0057401	87.500	40	5	0	92	131	1	40	1.06e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1086730	ATCG00900.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1086730	ATCG01240.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1086730	ATMG01270.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5
Msa1086730	AT2G07696.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCATTCGTTGGTTATT+AGG	0.268444	7_2:-87543241	Msa1086730:CDS
CATCAAGTTCCCGTTGAAAT+AGG	0.307921	7_2:-87543283	Msa1086730:CDS
AAAACGTCCAGGTCGAAATA+TGG	0.317789	7_2:-87543209	Msa1086730:CDS
CAAATTAAGTTCCGAATTAG+TGG	0.342047	7_2:-87543182	Msa1086730:CDS
TGGCGATGCCATACGCAAAA+AGG	0.366109	7_2:-87543143	Msa1086730:CDS
TTTGGCAGCATCCACTAATT+CGG	0.404543	7_2:+87543171	None:intergenic
ACGTATTGCTTGACGTAAAA+CGG	0.405511	7_2:+87543357	None:intergenic
GCTTGACGTAAAACGGATAG+TGG	0.418362	7_2:+87543364	None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG	0.427761	7_2:-87543313	Msa1086730:CDS
TATGGCATCGCCATTCCCTT+TGG	0.436234	7_2:+87543153	None:intergenic
TGTGTGGATCCTATTTCAAC+GGG	0.470768	7_2:+87543274	None:intergenic
TTATCTATCGAGCTATGAAA+AGG	0.512086	7_2:-87543411	Msa1086730:CDS
GAAAAGCACTTGCCATTCGT+TGG	0.526859	7_2:-87543249	Msa1086730:CDS
GTGGATGCTGCCAAAGGGAA+TGG	0.532446	7_2:-87543163	Msa1086730:CDS
GGCGCATCCCGAAAACGTCC+AGG	0.540732	7_2:-87543220	Msa1086730:CDS
AAAGGAAGAGACTCATAGAA+TGG	0.548096	7_2:-87543125	Msa1086730:CDS
TTGTGTGGATCCTATTTCAA+CGG	0.548545	7_2:+87543273	None:intergenic
AATTAGTGGATGCTGCCAAA+GGG	0.575951	7_2:-87543168	Msa1086730:CDS
GAATTAGTGGATGCTGCCAA+AGG	0.584526	7_2:-87543169	Msa1086730:CDS
AGAGACTCATAGAATGGCAG+AGG	0.593635	7_2:-87543119	Msa1086730:CDS
TTGAAAGCCATATTTCGACC+TGG	0.605724	7_2:+87543202	None:intergenic
TGCGCCTAATAACCAACGAA+TGG	0.620213	7_2:+87543237	None:intergenic
GTTAACCGTATTATGAAACA+CGG	0.650772	7_2:-87543457	Msa1086730:CDS
TTTACGTCAAGCAATACGTG+GGG	0.715577	7_2:-87543353	Msa1086730:CDS
GTTGAAATAGGATCCACACA+AGG	0.731975	7_2:-87543271	Msa1086730:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTTTCCGTGTTTCATAATA+CGG	+	chr7_2:87543104-87543123	None:intergenic	25.0%
	GTTAACCGTATTATGAAACA+CGG	-	chr7_2:87543096-87543115	Msa1086730:CDS	30.0%
	GAAACACGGAAAAAAATCAT+TGG	-	chr7_2:87543110-87543129	Msa1086730:CDS	30.0%
	TTATCTATCGAGCTATGAAA+AGG	-	chr7_2:87543142-87543161	Msa1086730:CDS	30.0%
!!	TCTTGCTTTTACTGCTATAT+CGG	+	chr7_2:87543232-87543251	None:intergenic	30.0%
	CAAATTAAGTTCCGAATTAG+TGG	-	chr7_2:87543371-87543390	Msa1086730:CDS	30.0%
	ACGTATTGCTTGACGTAAAA+CGG	+	chr7_2:87543199-87543218	None:intergenic	35.0%
!	TTTTACGTCAAGCAATACGT+GGG	-	chr7_2:87543199-87543218	Msa1086730:CDS	35.0%
!!	CTTGCTTTTACTGCTATATC+GGG	+	chr7_2:87543231-87543250	None:intergenic	35.0%
	TTGTGTGGATCCTATTTCAA+CGG	+	chr7_2:87543283-87543302	None:intergenic	35.0%
	AAAGGAAGAGACTCATAGAA+TGG	-	chr7_2:87543428-87543447	Msa1086730:CDS	35.0%
!	GTTTTACGTCAAGCAATACG+TGG	-	chr7_2:87543198-87543217	Msa1086730:CDS	40.0%
	TTTACGTCAAGCAATACGTG+GGG	-	chr7_2:87543200-87543219	Msa1086730:CDS	40.0%
	GTAAAAGCAAGACGCGTAAA+CGG	-	chr7_2:87543240-87543259	Msa1086730:CDS	40.0%
	CATCAAGTTCCCGTTGAAAT+AGG	-	chr7_2:87543270-87543289	Msa1086730:CDS	40.0%
	TGTGTGGATCCTATTTCAAC+GGG	+	chr7_2:87543282-87543301	None:intergenic	40.0%
	GTTGAAATAGGATCCACACA+AGG	-	chr7_2:87543282-87543301	Msa1086730:CDS	40.0%
!	CTTGCCATTCGTTGGTTATT+AGG	-	chr7_2:87543312-87543331	Msa1086730:CDS	40.0%
!	TATTTCGACCTGGACGTTTT+CGG	+	chr7_2:87543344-87543363	None:intergenic	40.0%
	AAAACGTCCAGGTCGAAATA+TGG	-	chr7_2:87543344-87543363	Msa1086730:CDS	40.0%
	TTGAAAGCCATATTTCGACC+TGG	+	chr7_2:87543354-87543373	None:intergenic	40.0%
	TTTGGCAGCATCCACTAATT+CGG	+	chr7_2:87543385-87543404	None:intergenic	40.0%
!	AATTAGTGGATGCTGCCAAA+GGG	-	chr7_2:87543385-87543404	Msa1086730:CDS	40.0%
!	AGTCTCTTCCTTTTTGCGTA+TGG	+	chr7_2:87543421-87543440	None:intergenic	40.0%
	GCTTGACGTAAAACGGATAG+TGG	+	chr7_2:87543192-87543211	None:intergenic	45.0%
!!	GAAAAGCACTTGCCATTCGT+TGG	-	chr7_2:87543304-87543323	Msa1086730:CDS	45.0%
	TGCGCCTAATAACCAACGAA+TGG	+	chr7_2:87543319-87543338	None:intergenic	45.0%
!	ATTTCGACCTGGACGTTTTC+GGG	+	chr7_2:87543343-87543362	None:intergenic	45.0%
!	GAATTAGTGGATGCTGCCAA+AGG	-	chr7_2:87543384-87543403	Msa1086730:CDS	45.0%
	AGAGACTCATAGAATGGCAG+AGG	-	chr7_2:87543434-87543453	Msa1086730:CDS	45.0%
!	GGCAAGTGCTTTTCCTTGTG+TGG	+	chr7_2:87543298-87543317	None:intergenic	50.0%
	TATGGCATCGCCATTCCCTT+TGG	+	chr7_2:87543403-87543422	None:intergenic	50.0%
	TGGCGATGCCATACGCAAAA+AGG	-	chr7_2:87543410-87543429	Msa1086730:CDS	50.0%
	GTGGATGCTGCCAAAGGGAA+TGG	-	chr7_2:87543390-87543409	Msa1086730:CDS	55.0%
	GGCGCATCCCGAAAACGTCC+AGG	-	chr7_2:87543333-87543352	Msa1086730:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	87543090	87543485	87543090	ID=Msa1086730;Name=Msa1086730
chr7_2	mRNA	87543090	87543485	87543090	ID=Msa1086730-mRNA-1;Parent=Msa1086730;Name=Msa1086730-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|131
chr7_2	exon	87543090	87543485	87543090	ID=Msa1086730-mRNA-1:exon:23858;Parent=Msa1086730-mRNA-1
chr7_2	CDS	87543090	87543485	87543090	ID=Msa1086730-mRNA-1:cds;Parent=Msa1086730-mRNA-1

Gene Sequence

>Msa1086730

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1086730

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*