AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368230 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1368230 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1368230 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1368230 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa1368230 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368230 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1368230 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1368230 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa1368230 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0018631:-1077 Msa1368230:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0018631:-1119 Msa1368230:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0018631:-1045 Msa1368230:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0018631:-1018 Msa1368230:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0018631:-979 Msa1368230:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 tig0018631:+1007 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0018631:+1193 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0018631:+1200 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0018631:-1149 Msa1368230:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 tig0018631:+989 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0018631:+1110 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0018631:-1247 Msa1368230:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0018631:-1085 Msa1368230:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 tig0018631:-999 Msa1368230:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0018631:-1056 Msa1368230:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0018631:-961 Msa1368230:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0018631:+1109 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 tig0018631:-1004 Msa1368230:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 tig0018631:-1005 Msa1368230:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0018631:-955 Msa1368230:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0018631:+1038 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0018631:+1073 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0018631:-1293 Msa1368230:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 tig0018631:-1189 Msa1368230:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0018631:-1107 Msa1368230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + tig0018631:940-959 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - tig0018631:932-951 Msa1368230:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - tig0018631:946-965 Msa1368230:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - tig0018631:978-997 Msa1368230:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + tig0018631:1068-1087 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - tig0018631:1207-1226 Msa1368230:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + tig0018631:1035-1054 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - tig0018631:1035-1054 Msa1368230:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + tig0018631:1067-1086 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + tig0018631:1119-1138 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - tig0018631:1264-1283 Msa1368230:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - tig0018631:1034-1053 Msa1368230:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - tig0018631:1036-1055 Msa1368230:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - tig0018631:1076-1095 Msa1368230:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - tig0018631:1106-1125 Msa1368230:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + tig0018631:1118-1137 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - tig0018631:1118-1137 Msa1368230:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - tig0018631:1148-1167 Msa1368230:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + tig0018631:1180-1199 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - tig0018631:1180-1199 Msa1368230:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + tig0018631:1190-1209 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + tig0018631:1221-1240 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - tig0018631:1221-1240 Msa1368230:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + tig0018631:1257-1276 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + tig0018631:1028-1047 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - tig0018631:1140-1159 Msa1368230:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + tig0018631:1155-1174 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + tig0018631:1179-1198 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - tig0018631:1220-1239 Msa1368230:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - tig0018631:1270-1289 Msa1368230:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + tig0018631:1134-1153 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + tig0018631:1239-1258 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - tig0018631:1246-1265 Msa1368230:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - tig0018631:1226-1245 Msa1368230:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - tig0018631:1169-1188 Msa1368230:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018631 gene 926 1321 926 ID=Msa1368230;Name=Msa1368230
tig0018631 mRNA 926 1321 926 ID=Msa1368230-mRNA-1;Parent=Msa1368230;Name=Msa1368230-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
tig0018631 exon 926 1321 926 ID=Msa1368230-mRNA-1:exon:385;Parent=Msa1368230-mRNA-1
tig0018631 CDS 926 1321 926 ID=Msa1368230-mRNA-1:cds;Parent=Msa1368230-mRNA-1
Gene Sequence

>Msa1368230

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1368230

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*