AlfalfaGEDB | Msa1087040

M. sativa cultivar zhongmu-4 / Msa1087040

KEGG: K02992
GO: GO:0000028 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00177.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1087040	YP_009141653.1	100.000	131	0	0	1	131	25	155	1.21e-90	269

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1087040	sp\|P07135\|RR7_SOYBN	96.947	131	4	0	1	131	25	155	1.13e-90	262

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1087040	A0A0F6NLW0	100.000	131	0	0	1	131	25	155	5.78e-91	269

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095190	Msa1087040	0.815156	1.068228e-51	-8.615850e-47
Msa0202640	Msa1087040	0.828783	7.420081e-55	-8.615850e-47
Msa0370210	Msa1087040	0.822237	2.637692e-53	-8.615850e-47
Msa0398630	Msa1087040	0.817606	3.021993e-52	-8.615850e-47
Msa0405420	Msa1087040	0.847488	1.120651e-59	-8.615850e-47
Msa0405430	Msa1087040	0.870300	1.619553e-66	-8.615850e-47
Msa0405440	Msa1087040	0.847460	1.140370e-59	-8.615850e-47
Msa0405510	Msa1087040	0.860643	1.790282e-63	-8.615850e-47
Msa0405520	Msa1087040	0.855581	5.732399e-62	-8.615850e-47
Msa0405530	Msa1087040	0.839014	2.040693e-57	-8.615850e-47
Msa0983630	Msa1087040	0.885007	1.177915e-71	-8.615850e-47
Msa0983640	Msa1087040	0.866514	2.697127e-65	-8.615850e-47
Msa0983720	Msa1087040	0.814600	1.418324e-51	-8.615850e-47
Msa1022050	Msa1087040	0.875828	2.264311e-68	-8.615850e-47
Msa1022060	Msa1087040	0.841123	5.752093e-58	-8.615850e-47
Msa1064410	Msa1087040	0.854938	8.820169e-62	-8.615850e-47
Msa1086070	Msa1087040	0.824832	6.519314e-54	-8.615850e-47
Msa1086080	Msa1087040	0.844818	5.971431e-59	-8.615850e-47
Msa1086090	Msa1087040	0.822341	2.495246e-53	-8.615850e-47
Msa1086100	Msa1087040	0.820018	8.561241e-53	-8.615850e-47
Msa1086140	Msa1087040	0.847341	1.229481e-59	-8.615850e-47
Msa1086150	Msa1087040	0.817950	2.527032e-52	-8.615850e-47
Msa1086180	Msa1087040	0.821788	3.350747e-53	-8.615850e-47
Msa1086190	Msa1087040	0.847075	1.454547e-59	-8.615850e-47
Msa1086200	Msa1087040	0.826446	2.701631e-54	-8.615850e-47
Msa1086210	Msa1087040	0.815390	9.472939e-52	-8.615850e-47
Msa1086270	Msa1087040	0.845593	3.687746e-59	-8.615850e-47
Msa1086280	Msa1087040	0.821064	4.924107e-53	-8.615850e-47
Msa1086380	Msa1087040	0.829928	3.911333e-55	-8.615850e-47
Msa1086450	Msa1087040	0.815322	9.811411e-52	-8.615850e-47
Msa1086470	Msa1087040	0.803569	3.274616e-49	-8.615850e-47
Msa1086550	Msa1087040	0.859680	3.498482e-63	-8.615850e-47
Msa1086560	Msa1087040	0.822514	2.274378e-53	-8.615850e-47
Msa1086590	Msa1087040	0.877683	5.161125e-69	-8.615850e-47
Msa1086600	Msa1087040	0.864911	8.645056e-65	-8.615850e-47
Msa1086720	Msa1087040	0.865162	7.211575e-65	-8.615850e-47
Msa1086730	Msa1087040	0.933527	1.639538e-95	-8.615850e-47
Msa1086740	Msa1087040	0.855967	4.419781e-62	-8.615850e-47
Msa1086790	Msa1087040	0.894282	2.855620e-75	-8.615850e-47
Msa1086800	Msa1087040	0.887542	1.302222e-72	-8.615850e-47
Msa1086880	Msa1087040	0.829303	5.551462e-55	-8.615850e-47
Msa1086890	Msa1087040	0.849939	2.343071e-60	-8.615850e-47
Msa1086900	Msa1087040	0.824175	9.304832e-54	-8.615850e-47
Msa1086910	Msa1087040	0.824685	7.061310e-54	-8.615850e-47
Msa1086960	Msa1087040	0.871293	7.633459e-67	-8.615850e-47
Msa1086970	Msa1087040	0.852057	5.926651e-61	-8.615850e-47
Msa1087030	Msa1087040	0.860728	1.687338e-63	-8.615850e-47
Msa1087040	Msa1087050	0.855629	5.548261e-62	-8.615850e-47
Msa1087040	Msa1087110	0.890522	9.133543e-74	-8.615850e-47
Msa1087040	Msa1087120	0.861283	1.143892e-63	-8.615850e-47
Msa1087040	Msa1143120	0.857476	1.590867e-62	-8.615850e-47
Msa1087040	Msa1143130	0.827977	1.160947e-54	-8.615850e-47
Msa1087040	Msa1143160	0.829565	4.795295e-55	-8.615850e-47
Msa1087040	Msa1143170	0.812259	4.638316e-51	-8.615850e-47
Msa1087040	Msa1143180	0.819420	1.172661e-52	-8.615850e-47
Msa1087040	Msa1305090	0.842266	2.872018e-58	-8.615850e-47
Msa1087040	Msa1354320	0.808387	3.175454e-50	-8.615850e-47
Msa1087040	Msa1354380	0.805099	1.571989e-49	-8.615850e-47
Msa1087040	Msa1354420	0.889262	2.835371e-73	-8.615850e-47
Msa1087040	Msa1354430	0.868479	6.331377e-66	-8.615850e-47
Msa1087040	Msa1354480	0.862070	6.575639e-64	-8.615850e-47
Msa1087040	Msa1354490	0.904548	1.093624e-79	-8.615850e-47
Msa1087040	Msa1354500	0.863759	1.978612e-64	-8.615850e-47
Msa1087040	Msa1354550	0.900187	9.414041e-78	-8.615850e-47
Msa1087040	Msa1354560	0.848500	5.890877e-60	-8.615850e-47
Msa1087040	Msa1354610	0.882963	6.703082e-71	-8.615850e-47
Msa1087040	Msa1354620	0.891679	3.187476e-74	-8.615850e-47
Msa1087040	Msa1354630	0.875054	4.169814e-68	-8.615850e-47
Msa1087040	Msa1354680	0.842370	2.695077e-58	-8.615850e-47
Msa1087040	Msa1354690	0.890513	9.206752e-74	-8.615850e-47
Msa1087040	Msa1354700	0.847312	1.251915e-59	-8.615850e-47
Msa1087040	Msa1354740	0.861084	1.314681e-63	-8.615850e-47
Msa1087040	Msa1354750	0.920632	1.000712e-87	-8.615850e-47
Msa1087040	Msa1354760	0.866022	3.861575e-65	-8.615850e-47
Msa1087040	Msa1354810	0.889398	2.510711e-73	-8.615850e-47
Msa1087040	Msa1354820	0.861758	8.191002e-64	-8.615850e-47
Msa1087040	Msa1354900	0.828180	1.037366e-54	-8.615850e-47
Msa1087040	Msa1354910	0.844134	9.126775e-59	-8.615850e-47
Msa1087040	Msa1354920	0.823295	1.495791e-53	-8.615850e-47
Msa1087040	Msa1354930	0.820416	6.938746e-53	-8.615850e-47
Msa1087040	Msa1354980	0.884831	1.369830e-71	-8.615850e-47
Msa1087040	Msa1354990	0.855008	8.412820e-62	-8.615850e-47
Msa1087040	Msa1355070	0.822514	2.274582e-53	-8.615850e-47
Msa1087040	Msa1355080	0.840276	9.584599e-58	-8.615850e-47
Msa1087040	Msa1355090	0.822519	2.268614e-53	-8.615850e-47
Msa1087040	Msa1355100	0.814352	1.609775e-51	-8.615850e-47
Msa1087040	Msa1365890	0.873354	1.569350e-67	-8.615850e-47
Msa1087040	Msa1365900	0.864023	1.638487e-64	-8.615850e-47
Msa1087040	Msa1365950	0.856533	3.016749e-62	-8.615850e-47
Msa1087040	Msa1365960	0.897528	1.287976e-76	-8.615850e-47
Msa1087040	Msa1365970	0.838626	2.572133e-57	-8.615850e-47
Msa1087040	Msa1366080	0.894001	3.716970e-75	-8.615850e-47
Msa1087040	Msa1366090	0.851623	7.867574e-61	-8.615850e-47
Msa1087040	Msa1368230	0.881795	1.783445e-70	-8.615850e-47
Msa1087040	Msa1368240	0.839925	1.183456e-57	-8.615850e-47
Msa1087040	Msa1380590	0.805876	1.080356e-49	-8.615850e-47
Msa1087040	Msa1380600	0.817789	2.748201e-52	-8.615850e-47
Msa1087040	Msa1380620	0.815091	1.104040e-51	-8.615850e-47
Msa1087040	Msa1385410	0.873756	1.148722e-67	-8.615850e-47
Msa1087040	Msa1385420	0.818488	1.909531e-52	-8.615850e-47
Msa1087040	Msa1391090	0.856370	3.369319e-62	-8.615850e-47
Msa1087040	Msa1391100	0.916019	2.943882e-85	-8.615850e-47
Msa1087040	Msa1391110	0.843125	1.697858e-58	-8.615850e-47
Msa1087040	Msa1391160	0.879672	1.029583e-69	-8.615850e-47
Msa1087040	Msa1391170	0.868219	7.684750e-66	-8.615850e-47
Msa1087040	Msa1391220	0.858228	9.515125e-63	-8.615850e-47
Msa1087040	Msa1391230	0.915945	3.216677e-85	-8.615850e-47
Msa1087040	Msa1391240	0.854788	9.746950e-62	-8.615850e-47
Msa1087040	Msa1394030	0.821807	3.316973e-53	-8.615850e-47
Msa1087040	Msa1394040	0.848594	5.548338e-60	-8.615850e-47
Msa1087040	Msa1394150	0.851463	8.734351e-61	-8.615850e-47
Msa1087040	Msa1394160	0.823064	1.693620e-53	-8.615850e-47
Msa1087040	Msa1394210	0.834256	3.330004e-56	-8.615850e-47
Msa1087040	Msa1394220	0.812313	4.515336e-51	-8.615850e-47
Msa1087040	Msa1394230	0.815684	8.149733e-52	-8.615850e-47
Msa1087040	Msa1460120	0.827048	1.939453e-54	-8.615850e-47
Msa1087040	Msa1460130	0.854348	1.307114e-61	-8.615850e-47
Msa0263770	Msa1087040	0.838459	2.840225e-57	-8.615850e-47
Msa0268170	Msa1087040	0.825417	4.741309e-54	-8.615850e-47
Msa0268250	Msa1087040	0.850047	2.185565e-60	-8.615850e-47
Msa0268260	Msa1087040	0.919801	2.857270e-87	-8.615850e-47
Msa0268270	Msa1087040	0.860554	1.905495e-63	-8.615850e-47
Msa0268300	Msa1087040	0.879702	1.004356e-69	-8.615850e-47
Msa0268310	Msa1087040	0.858727	6.754026e-63	-8.615850e-47
Msa0268390	Msa1087040	0.827481	1.528038e-54	-8.615850e-47
Msa0268400	Msa1087040	0.809602	1.744549e-50	-8.615850e-47
Msa0268410	Msa1087040	0.821923	3.118313e-53	-8.615850e-47
Msa0268550	Msa1087040	0.830622	2.648244e-55	-8.615850e-47
Msa0268560	Msa1087040	0.839847	1.240384e-57	-8.615850e-47
Msa0268570	Msa1087040	0.815390	9.474931e-52	-8.615850e-47
Msa0268580	Msa1087040	0.832734	7.984425e-56	-8.615850e-47
Msa0268630	Msa1087040	0.840227	9.869784e-58	-8.615850e-47
Msa0268640	Msa1087040	0.819521	1.111778e-52	-8.615850e-47
Msa0268740	Msa1087040	0.851720	7.382176e-61	-8.615850e-47
Msa0268750	Msa1087040	0.818289	2.118073e-52	-8.615850e-47
Msa0268760	Msa1087040	0.812452	4.209044e-51	-8.615850e-47
Msa0268830	Msa1087040	0.849646	2.828969e-60	-8.615850e-47
Msa0268840	Msa1087040	0.822383	2.440140e-53	-8.615850e-47
Msa0268940	Msa1087040	0.829471	5.052983e-55	-8.615850e-47
Msa0268950	Msa1087040	0.821931	3.104726e-53	-8.615850e-47
Msa0268960	Msa1087040	0.831759	1.391429e-55	-8.615850e-47
Msa0269040	Msa1087040	0.857860	1.224135e-62	-8.615850e-47
Msa0269050	Msa1087040	0.835066	2.083283e-56	-8.615850e-47
Msa0269130	Msa1087040	0.849477	3.153552e-60	-8.615850e-47
Msa0269140	Msa1087040	0.925539	1.599618e-90	-8.615850e-47
Msa0269150	Msa1087040	0.870453	1.443014e-66	-8.615850e-47
Msa0344180	Msa1087040	0.804640	1.960227e-49	-8.615850e-47
Msa0344190	Msa1087040	0.829757	4.304382e-55	-8.615850e-47
Msa0344300	Msa1087040	0.806557	7.765302e-50	-8.615850e-47
Msa0344310	Msa1087040	0.856784	2.545908e-62	-8.615850e-47
Msa0582510	Msa1087040	0.855379	6.561444e-62	-8.615850e-47
Msa0582520	Msa1087040	0.816215	6.202528e-52	-8.615850e-47
Msa0582630	Msa1087040	0.843900	1.054050e-58	-8.615850e-47
Msa0582640	Msa1087040	0.855044	8.216213e-62	-8.615850e-47
Msa0582650	Msa1087040	0.819043	1.428315e-52	-8.615850e-47
Msa0582660	Msa1087040	0.832311	1.016562e-55	-8.615850e-47
Msa0582690	Msa1087040	0.847498	1.113296e-59	-8.615850e-47
Msa0582700	Msa1087040	0.824567	7.528373e-54	-8.615850e-47
Msa0582750	Msa1087040	0.832109	1.140202e-55	-8.615850e-47
Msa0582760	Msa1087040	0.852743	3.778500e-61	-8.615850e-47
Msa0582770	Msa1087040	0.814935	1.195642e-51	-8.615850e-47
Msa0582780	Msa1087040	0.834515	2.867398e-56	-8.615850e-47
Msa0582810	Msa1087040	0.852183	5.455588e-61	-8.615850e-47
Msa0582820	Msa1087040	0.832876	7.357991e-56	-8.615850e-47
Msa0582910	Msa1087040	0.838876	2.216032e-57	-8.615850e-47
Msa0582920	Msa1087040	0.817536	3.134888e-52	-8.615850e-47
Msa0582930	Msa1087040	0.841864	3.669284e-58	-8.615850e-47
Msa0591290	Msa1087040	0.828035	1.124331e-54	-8.615850e-47
Msa0591310	Msa1087040	0.846806	1.722776e-59	-8.615850e-47
Msa0591350	Msa1087040	0.871768	5.312325e-67	-8.615850e-47
Msa0591360	Msa1087040	0.843070	1.756508e-58	-8.615850e-47
Msa0591380	Msa1087040	0.830579	2.712367e-55	-8.615850e-47
Msa0867680	Msa1087040	0.882686	8.461996e-71	-8.615850e-47
Msa0867690	Msa1087040	0.836470	9.186356e-57	-8.615850e-47
Msa0908220	Msa1087040	0.875107	3.998963e-68	-8.615850e-47
Msa0908230	Msa1087040	0.924133	1.057550e-89	-8.615850e-47
Msa0908240	Msa1087040	0.854240	1.404391e-61	-8.615850e-47
Msa0908310	Msa1087040	0.912194	2.573215e-83	-8.615850e-47
Msa0908320	Msa1087040	0.861203	1.210007e-63	-8.615850e-47
Msa0908370	Msa1087040	0.900239	8.939887e-78	-8.615850e-47
Msa0908380	Msa1087040	0.828077	1.098522e-54	-8.615850e-47
Msa0908430	Msa1087040	0.852883	3.446801e-61	-8.615850e-47
Msa0908440	Msa1087040	0.907694	3.840116e-81	-8.615850e-47
Msa0908450	Msa1087040	0.867342	1.468476e-65	-8.615850e-47
Msa0908480	Msa1087040	0.819306	1.244781e-52	-8.615850e-47
Msa0908530	Msa1087040	0.905101	6.127114e-80	-8.615850e-47
Msa0908540	Msa1087040	0.854438	1.230800e-61	-8.615850e-47
Msa0908590	Msa1087040	0.849661	2.801260e-60	-8.615850e-47
Msa0908600	Msa1087040	0.887391	1.486860e-72	-8.615850e-47
Msa0908610	Msa1087040	0.825836	3.771391e-54	-8.615850e-47
Msa0908650	Msa1087040	0.862134	6.281304e-64	-8.615850e-47
Msa0908660	Msa1087040	0.895508	8.966114e-76	-8.615850e-47
Msa0908670	Msa1087040	0.865514	5.587017e-65	-8.615850e-47
Msa0908720	Msa1087040	0.897170	1.823162e-76	-8.615850e-47
Msa0908730	Msa1087040	0.833744	4.473627e-56	-8.615850e-47
Msa0911190	Msa1087040	0.824870	6.386831e-54	-8.615850e-47
Msa0911200	Msa1087040	0.824076	9.819309e-54	-8.615850e-47
Msa0911230	Msa1087040	0.803379	3.584788e-49	-8.615850e-47
Msa0924830	Msa1087040	0.819505	1.121450e-52	-8.615850e-47
Msa0924870	Msa1087040	0.830036	3.680695e-55	-8.615850e-47
Msa0924880	Msa1087040	0.801216	9.999215e-49	-8.615850e-47
Msa0924890	Msa1087040	0.801588	8.390241e-49	-8.615850e-47
Msa0924930	Msa1087040	0.881654	2.005997e-70	-8.615850e-47
Msa0924940	Msa1087040	0.862215	5.934496e-64	-8.615850e-47
Msa0925100	Msa1087040	0.862481	4.916137e-64	-8.615850e-47
Msa0925110	Msa1087040	0.861643	8.881514e-64	-8.615850e-47
Msa0931070	Msa1087040	0.841367	4.960634e-58	-8.615850e-47
Msa0931080	Msa1087040	0.839388	1.632146e-57	-8.615850e-47
Msa0931130	Msa1087040	0.849270	3.601837e-60	-8.615850e-47
Msa0931140	Msa1087040	0.824211	9.125553e-54	-8.615850e-47
Msa0931210	Msa1087040	0.806537	7.839053e-50	-8.615850e-47
Msa0931220	Msa1087040	0.820571	6.392830e-53	-8.615850e-47
Msa0931240	Msa1087040	0.819592	1.071336e-52	-8.615850e-47
Msa0931260	Msa1087040	0.854258	1.387390e-61	-8.615850e-47
Msa0931270	Msa1087040	0.831302	1.802690e-55	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1087040	MtrunA17_CPg0492421	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1087040	MtrunA17_Chr4g0016101	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1087040	MtrunA17_Chr3g0090841	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1087040	MtrunA17_Chr4g0002541	83.333	60	10	0	1	60	1	60	3.60e-30	103
Msa1087040	MtrunA17_Chr4g0057401	87.500	40	5	0	92	131	1	40	1.06e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1087040	ATCG00900.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1087040	ATCG01240.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1087040	ATMG01270.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5
Msa1087040	AT2G07696.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCATTCGTTGGTTATT+AGG	0.268444	7_2:-87691587	Msa1087040:CDS
CATCAAGTTCCCGTTGAAAT+AGG	0.307921	7_2:-87691629	Msa1087040:CDS
AAAACGTCCAGGTCGAAATA+TGG	0.317789	7_2:-87691555	Msa1087040:CDS
CAAATTAAGTTCCGAATTAG+TGG	0.342047	7_2:-87691528	Msa1087040:CDS
TGGCGATGCCATACGCAAAA+AGG	0.366109	7_2:-87691489	Msa1087040:CDS
TTTGGCAGCATCCACTAATT+CGG	0.404543	7_2:+87691517	None:intergenic
ACGTATTGCTTGACGTAAAA+CGG	0.405511	7_2:+87691703	None:intergenic
GCTTGACGTAAAACGGATAG+TGG	0.418362	7_2:+87691710	None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG	0.427761	7_2:-87691659	Msa1087040:CDS
TATGGCATCGCCATTCCCTT+TGG	0.436234	7_2:+87691499	None:intergenic
TGTGTGGATCCTATTTCAAC+GGG	0.470768	7_2:+87691620	None:intergenic
TTATCTATCGAGCTATGAAA+AGG	0.512086	7_2:-87691757	Msa1087040:CDS
GAAAAGCACTTGCCATTCGT+TGG	0.526859	7_2:-87691595	Msa1087040:CDS
GTGGATGCTGCCAAAGGGAA+TGG	0.532446	7_2:-87691509	Msa1087040:CDS
GGCGCATCCCGAAAACGTCC+AGG	0.540732	7_2:-87691566	Msa1087040:CDS
AAAGGAAGAGACTCATAGAA+TGG	0.548096	7_2:-87691471	Msa1087040:CDS
TTGTGTGGATCCTATTTCAA+CGG	0.548545	7_2:+87691619	None:intergenic
AATTAGTGGATGCTGCCAAA+GGG	0.575951	7_2:-87691514	Msa1087040:CDS
GAATTAGTGGATGCTGCCAA+AGG	0.584526	7_2:-87691515	Msa1087040:CDS
AGAGACTCATAGAATGGCAG+AGG	0.593635	7_2:-87691465	Msa1087040:CDS
TTGAAAGCCATATTTCGACC+TGG	0.605724	7_2:+87691548	None:intergenic
TGCGCCTAATAACCAACGAA+TGG	0.620213	7_2:+87691583	None:intergenic
GTTAACCGTATTATGAAACA+CGG	0.650772	7_2:-87691803	Msa1087040:CDS
TTTACGTCAAGCAATACGTG+GGG	0.715577	7_2:-87691699	Msa1087040:CDS
GTTGAAATAGGATCCACACA+AGG	0.731975	7_2:-87691617	Msa1087040:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTTTCCGTGTTTCATAATA+CGG	+	chr7_2:87691450-87691469	None:intergenic	25.0%
	GTTAACCGTATTATGAAACA+CGG	-	chr7_2:87691442-87691461	Msa1087040:CDS	30.0%
	GAAACACGGAAAAAAATCAT+TGG	-	chr7_2:87691456-87691475	Msa1087040:CDS	30.0%
	TTATCTATCGAGCTATGAAA+AGG	-	chr7_2:87691488-87691507	Msa1087040:CDS	30.0%
!!	TCTTGCTTTTACTGCTATAT+CGG	+	chr7_2:87691578-87691597	None:intergenic	30.0%
	CAAATTAAGTTCCGAATTAG+TGG	-	chr7_2:87691717-87691736	Msa1087040:CDS	30.0%
	ACGTATTGCTTGACGTAAAA+CGG	+	chr7_2:87691545-87691564	None:intergenic	35.0%
!	TTTTACGTCAAGCAATACGT+GGG	-	chr7_2:87691545-87691564	Msa1087040:CDS	35.0%
!!	CTTGCTTTTACTGCTATATC+GGG	+	chr7_2:87691577-87691596	None:intergenic	35.0%
	TTGTGTGGATCCTATTTCAA+CGG	+	chr7_2:87691629-87691648	None:intergenic	35.0%
	AAAGGAAGAGACTCATAGAA+TGG	-	chr7_2:87691774-87691793	Msa1087040:CDS	35.0%
!	GTTTTACGTCAAGCAATACG+TGG	-	chr7_2:87691544-87691563	Msa1087040:CDS	40.0%
	TTTACGTCAAGCAATACGTG+GGG	-	chr7_2:87691546-87691565	Msa1087040:CDS	40.0%
	GTAAAAGCAAGACGCGTAAA+CGG	-	chr7_2:87691586-87691605	Msa1087040:CDS	40.0%
	CATCAAGTTCCCGTTGAAAT+AGG	-	chr7_2:87691616-87691635	Msa1087040:CDS	40.0%
	TGTGTGGATCCTATTTCAAC+GGG	+	chr7_2:87691628-87691647	None:intergenic	40.0%
	GTTGAAATAGGATCCACACA+AGG	-	chr7_2:87691628-87691647	Msa1087040:CDS	40.0%
!	CTTGCCATTCGTTGGTTATT+AGG	-	chr7_2:87691658-87691677	Msa1087040:CDS	40.0%
!	TATTTCGACCTGGACGTTTT+CGG	+	chr7_2:87691690-87691709	None:intergenic	40.0%
	AAAACGTCCAGGTCGAAATA+TGG	-	chr7_2:87691690-87691709	Msa1087040:CDS	40.0%
	TTGAAAGCCATATTTCGACC+TGG	+	chr7_2:87691700-87691719	None:intergenic	40.0%
	TTTGGCAGCATCCACTAATT+CGG	+	chr7_2:87691731-87691750	None:intergenic	40.0%
!	AATTAGTGGATGCTGCCAAA+GGG	-	chr7_2:87691731-87691750	Msa1087040:CDS	40.0%
!	AGTCTCTTCCTTTTTGCGTA+TGG	+	chr7_2:87691767-87691786	None:intergenic	40.0%
	GCTTGACGTAAAACGGATAG+TGG	+	chr7_2:87691538-87691557	None:intergenic	45.0%
!!	GAAAAGCACTTGCCATTCGT+TGG	-	chr7_2:87691650-87691669	Msa1087040:CDS	45.0%
	TGCGCCTAATAACCAACGAA+TGG	+	chr7_2:87691665-87691684	None:intergenic	45.0%
!	ATTTCGACCTGGACGTTTTC+GGG	+	chr7_2:87691689-87691708	None:intergenic	45.0%
!	GAATTAGTGGATGCTGCCAA+AGG	-	chr7_2:87691730-87691749	Msa1087040:CDS	45.0%
	AGAGACTCATAGAATGGCAG+AGG	-	chr7_2:87691780-87691799	Msa1087040:CDS	45.0%
!	GGCAAGTGCTTTTCCTTGTG+TGG	+	chr7_2:87691644-87691663	None:intergenic	50.0%
	TATGGCATCGCCATTCCCTT+TGG	+	chr7_2:87691749-87691768	None:intergenic	50.0%
	TGGCGATGCCATACGCAAAA+AGG	-	chr7_2:87691756-87691775	Msa1087040:CDS	50.0%
	GTGGATGCTGCCAAAGGGAA+TGG	-	chr7_2:87691736-87691755	Msa1087040:CDS	55.0%
	GGCGCATCCCGAAAACGTCC+AGG	-	chr7_2:87691679-87691698	Msa1087040:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	87691436	87691831	87691436	ID=Msa1087040;Name=Msa1087040
chr7_2	mRNA	87691436	87691831	87691436	ID=Msa1087040-mRNA-1;Parent=Msa1087040;Name=Msa1087040-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|131
chr7_2	exon	87691436	87691831	87691436	ID=Msa1087040-mRNA-1:exon:23983;Parent=Msa1087040-mRNA-1
chr7_2	CDS	87691436	87691831	87691436	ID=Msa1087040-mRNA-1:cds;Parent=Msa1087040-mRNA-1

Gene Sequence

>Msa1087040

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1087040

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*