AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0908440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908440 MtrunA17_CPg0492421 92.994 157 9 1 1 155 1 157 3.39e-104 294
Msa0908440 MtrunA17_Chr4g0016101 92.994 157 9 1 1 155 1 157 3.39e-104 294
Msa0908440 MtrunA17_Chr3g0090841 92.994 157 9 1 1 155 1 157 3.39e-104 294
Msa0908440 MtrunA17_Chr4g0002541 83.333 60 10 0 25 84 1 60 7.63e-30 103
Msa0908440 MtrunA17_Chr4g0057401 87.500 40 5 0 116 155 1 40 2.03e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908440 ATCG00900.1 95.484 155 7 0 1 155 1 155 3.40e-109 307
Msa0908440 ATCG01240.1 95.484 155 7 0 1 155 1 155 3.40e-109 307
Msa0908440 ATMG01270.1 33.571 140 83 3 22 155 11 146 1.72e-18 77.4
Msa0908440 AT2G07696.1 33.571 140 83 3 22 155 11 146 1.72e-18 77.4

Find 29 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAATCGATTACGATAAAT+TGG 0.238141 6_2:+9606652 None:intergenic
CTTGCCATTCGTTGGTTATT+AGG 0.268444 6_2:-9606403 Msa0908440:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 6_2:-9606445 Msa0908440:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 6_2:-9606371 Msa0908440:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 6_2:-9606344 Msa0908440:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 6_2:-9606305 Msa0908440:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 6_2:+9606333 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 6_2:+9606519 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 6_2:+9606526 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 6_2:-9606475 Msa0908440:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 6_2:+9606315 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 6_2:+9606436 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 6_2:-9606573 Msa0908440:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 6_2:-9606411 Msa0908440:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 6_2:-9606325 Msa0908440:CDS
TAATCGATTAGTTAACATGT+TGG 0.538486 6_2:-9606641 Msa0908440:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 6_2:-9606382 Msa0908440:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 6_2:-9606287 Msa0908440:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 6_2:+9606435 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 6_2:-9606330 Msa0908440:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 6_2:-9606331 Msa0908440:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 6_2:-9606281 Msa0908440:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 6_2:+9606364 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 6_2:+9606399 None:intergenic
TCGATTACGATAAATTGGAT+CGG 0.627987 6_2:+9606657 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 6_2:-9606619 Msa0908440:CDS
TTTACGCTCATGTCACGGCG+AGG 0.672550 6_2:-9606706 None:intergenic
TTTACGTCAAGCAATACGTG+GGG 0.715577 6_2:-9606515 Msa0908440:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 6_2:-9606433 Msa0908440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTAATCGATTACGATAAAT+TGG + chr6_2:9606300-9606319 None:intergenic 25.0%
!! TAATCGATTAGTTAACATGT+TGG - chr6_2:9606308-9606327 Msa0908440:CDS 25.0%
!! TTTTTCCGTGTTTCATAATA+CGG + chr6_2:9606338-9606357 None:intergenic 25.0%
! ATAAATTGGATCGGATTTTG+CGG + chr6_2:9606286-9606305 None:intergenic 30.0%
TCGATTACGATAAATTGGAT+CGG + chr6_2:9606295-9606314 None:intergenic 30.0%
GTTAACCGTATTATGAAACA+CGG - chr6_2:9606330-9606349 Msa0908440:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - chr6_2:9606344-9606363 Msa0908440:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - chr6_2:9606376-9606395 Msa0908440:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + chr6_2:9606466-9606485 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - chr6_2:9606605-9606624 Msa0908440:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + chr6_2:9606433-9606452 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - chr6_2:9606433-9606452 Msa0908440:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + chr6_2:9606465-9606484 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + chr6_2:9606517-9606536 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - chr6_2:9606662-9606681 Msa0908440:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - chr6_2:9606432-9606451 Msa0908440:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - chr6_2:9606434-9606453 Msa0908440:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - chr6_2:9606474-9606493 Msa0908440:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - chr6_2:9606504-9606523 Msa0908440:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + chr6_2:9606516-9606535 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - chr6_2:9606516-9606535 Msa0908440:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - chr6_2:9606546-9606565 Msa0908440:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + chr6_2:9606578-9606597 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - chr6_2:9606578-9606597 Msa0908440:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + chr6_2:9606588-9606607 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + chr6_2:9606619-9606638 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - chr6_2:9606619-9606638 Msa0908440:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + chr6_2:9606655-9606674 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + chr6_2:9606426-9606445 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - chr6_2:9606538-9606557 Msa0908440:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + chr6_2:9606553-9606572 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + chr6_2:9606577-9606596 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - chr6_2:9606618-9606637 Msa0908440:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - chr6_2:9606668-9606687 Msa0908440:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + chr6_2:9606532-9606551 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + chr6_2:9606637-9606656 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - chr6_2:9606644-9606663 Msa0908440:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - chr6_2:9606624-9606643 Msa0908440:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - chr6_2:9606567-9606586 Msa0908440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 9606252 9606719 9606252 ID=Msa0908440;Name=Msa0908440
chr6_2 mRNA 9606252 9606719 9606252 ID=Msa0908440-mRNA-1;Parent=Msa0908440;Name=Msa0908440-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|155
chr6_2 exon 9606252 9606719 9606252 ID=Msa0908440-mRNA-1:exon:2341;Parent=Msa0908440-mRNA-1
chr6_2 CDS 9606252 9606719 9606252 ID=Msa0908440-mRNA-1:cds;Parent=Msa0908440-mRNA-1
Gene Sequence

>Msa0908440

ATGTCACGGCGAGGTACTGCAGAAAAAAAAACCGCAAAATCCGATCCAATTTATCGTAATCGATTAGTTAACATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa0908440

MSRRGTAEKKTAKSDPIYRNRLVNMLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*