AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1391230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391230 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391230 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391230 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1391230 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa1391230 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1391230 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1391230 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1391230 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa1391230 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0021410:-106359 Msa1391230:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0021410:-106401 Msa1391230:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0021410:-106327 Msa1391230:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0021410:-106300 Msa1391230:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0021410:-106261 Msa1391230:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 tig0021410:+106289 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0021410:+106475 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0021410:+106482 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0021410:-106431 Msa1391230:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 tig0021410:+106271 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0021410:+106392 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0021410:-106529 Msa1391230:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0021410:-106367 Msa1391230:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 tig0021410:-106281 Msa1391230:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0021410:-106338 Msa1391230:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0021410:-106243 Msa1391230:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0021410:+106391 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 tig0021410:-106286 Msa1391230:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 tig0021410:-106287 Msa1391230:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0021410:-106237 Msa1391230:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0021410:+106320 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0021410:+106355 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0021410:-106575 Msa1391230:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 tig0021410:-106471 Msa1391230:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0021410:-106389 Msa1391230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + tig0021410:106222-106241 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - tig0021410:106214-106233 Msa1391230:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - tig0021410:106228-106247 Msa1391230:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - tig0021410:106260-106279 Msa1391230:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + tig0021410:106350-106369 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - tig0021410:106489-106508 Msa1391230:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + tig0021410:106317-106336 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - tig0021410:106317-106336 Msa1391230:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + tig0021410:106349-106368 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + tig0021410:106401-106420 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - tig0021410:106546-106565 Msa1391230:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - tig0021410:106316-106335 Msa1391230:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - tig0021410:106318-106337 Msa1391230:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - tig0021410:106358-106377 Msa1391230:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - tig0021410:106388-106407 Msa1391230:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + tig0021410:106400-106419 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - tig0021410:106400-106419 Msa1391230:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - tig0021410:106430-106449 Msa1391230:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + tig0021410:106462-106481 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - tig0021410:106462-106481 Msa1391230:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + tig0021410:106472-106491 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + tig0021410:106503-106522 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - tig0021410:106503-106522 Msa1391230:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + tig0021410:106539-106558 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + tig0021410:106310-106329 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - tig0021410:106422-106441 Msa1391230:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + tig0021410:106437-106456 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + tig0021410:106461-106480 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - tig0021410:106502-106521 Msa1391230:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - tig0021410:106552-106571 Msa1391230:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + tig0021410:106416-106435 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + tig0021410:106521-106540 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - tig0021410:106528-106547 Msa1391230:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - tig0021410:106508-106527 Msa1391230:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - tig0021410:106451-106470 Msa1391230:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0021410 gene 106208 106603 106208 ID=Msa1391230;Name=Msa1391230
tig0021410 mRNA 106208 106603 106208 ID=Msa1391230-mRNA-1;Parent=Msa1391230;Name=Msa1391230-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
tig0021410 exon 106208 106603 106208 ID=Msa1391230-mRNA-1:exon:12541;Parent=Msa1391230-mRNA-1
tig0021410 CDS 106208 106603 106208 ID=Msa1391230-mRNA-1:cds;Parent=Msa1391230-mRNA-1
Gene Sequence

>Msa1391230

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1391230

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*