AlfalfaGEDB | Msa1354620

M. sativa cultivar zhongmu-4 / Msa1354620

KEGG: K02992
GO: GO:0000028 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00177.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1354620	YP_009141653.1	100.000	131	0	0	1	131	25	155	1.21e-90	269

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1354620	sp\|P07135\|RR7_SOYBN	96.947	131	4	0	1	131	25	155	1.13e-90	262

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1354620	A0A0F6NLW0	100.000	131	0	0	1	131	25	155	5.78e-91	269

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095190	Msa1354620	0.814246	1.698715e-51	-8.615850e-47
Msa1354320	Msa1354620	0.804401	2.199275e-49	-8.615850e-47
Msa1354420	Msa1354620	0.905212	5.450568e-80	-8.615850e-47
Msa1354430	Msa1354620	0.861803	7.938249e-64	-8.615850e-47
Msa1354480	Msa1354620	0.871190	8.252772e-67	-8.615850e-47
Msa1354490	Msa1354620	0.927450	1.154945e-91	-8.615850e-47
Msa1354500	Msa1354620	0.869471	3.020158e-66	-8.615850e-47
Msa1354550	Msa1354620	0.915936	3.252253e-85	-8.615850e-47
Msa1354560	Msa1354620	0.854898	9.054901e-62	-8.615850e-47
Msa1354610	Msa1354620	0.880770	4.175529e-70	-8.615850e-47
Msa1354620	Msa1354630	0.879798	9.286708e-70	-8.615850e-47
Msa1354620	Msa1354680	0.849130	3.939284e-60	-8.615850e-47
Msa1354620	Msa1354690	0.917795	3.435369e-86	-8.615850e-47
Msa1354620	Msa1354700	0.835747	1.401676e-56	-8.615850e-47
Msa1354620	Msa1354740	0.864750	9.711250e-65	-8.615850e-47
Msa1354620	Msa1354750	0.933701	1.255426e-95	-8.615850e-47
Msa1354620	Msa1354760	0.861740	8.298847e-64	-8.615850e-47
Msa1354620	Msa1354810	0.912249	2.417325e-83	-8.615850e-47
Msa1354620	Msa1354820	0.864850	9.035277e-65	-8.615850e-47
Msa1354620	Msa1354900	0.814076	1.851743e-51	-8.615850e-47
Msa1354620	Msa1354910	0.831326	1.779017e-55	-8.615850e-47
Msa1354620	Msa1354920	0.828772	7.464918e-55	-8.615850e-47
Msa1354620	Msa1354930	0.830191	3.374183e-55	-8.615850e-47
Msa1354620	Msa1354980	0.904658	9.746639e-80	-8.615850e-47
Msa1354620	Msa1354990	0.866934	1.983113e-65	-8.615850e-47
Msa1354620	Msa1355070	0.803104	4.088331e-49	-8.615850e-47
Msa1354620	Msa1355080	0.825029	5.858059e-54	-8.615850e-47
Msa1354620	Msa1355090	0.823448	1.377521e-53	-8.615850e-47
Msa1354620	Msa1355100	0.813559	2.408042e-51	-8.615850e-47
Msa1354620	Msa1365890	0.878326	3.075329e-69	-8.615850e-47
Msa1354620	Msa1365900	0.835573	1.550743e-56	-8.615850e-47
Msa1354620	Msa1365950	0.865886	4.264424e-65	-8.615850e-47
Msa1354620	Msa1365960	0.891183	5.010378e-74	-8.615850e-47
Msa1354620	Msa1365970	0.862437	5.071724e-64	-8.615850e-47
Msa1354620	Msa1366080	0.907283	5.990190e-81	-8.615850e-47
Msa1354620	Msa1366090	0.834121	3.599796e-56	-8.615850e-47
Msa1354620	Msa1368230	0.913611	5.035989e-84	-8.615850e-47
Msa1354620	Msa1368240	0.854144	1.496227e-61	-8.615850e-47
Msa1354620	Msa1380590	0.800998	1.107570e-48	-8.615850e-47
Msa1354620	Msa1380600	0.833734	4.497549e-56	-8.615850e-47
Msa1354620	Msa1380620	0.825417	4.740503e-54	-8.615850e-47
Msa1354620	Msa1385410	0.897010	2.127888e-76	-8.615850e-47
Msa1354620	Msa1385420	0.812979	3.227099e-51	-8.615850e-47
Msa1354620	Msa1391090	0.884435	1.922232e-71	-8.615850e-47
Msa1354620	Msa1391100	0.918986	7.910296e-87	-8.615850e-47
Msa1354620	Msa1391110	0.855671	5.395504e-62	-8.615850e-47
Msa1354620	Msa1391160	0.894761	1.819372e-75	-8.615850e-47
Msa1354620	Msa1391170	0.844054	9.586932e-59	-8.615850e-47
Msa1354620	Msa1391220	0.874342	7.277506e-68	-8.615850e-47
Msa1354620	Msa1391230	0.910796	1.254546e-82	-8.615850e-47
Msa1354620	Msa1391240	0.882552	9.466913e-71	-8.615850e-47
Msa1354620	Msa1394030	0.829996	3.764659e-55	-8.615850e-47
Msa1354620	Msa1394040	0.843025	1.805655e-58	-8.615850e-47
Msa1354620	Msa1394150	0.831376	1.728546e-55	-8.615850e-47
Msa1354620	Msa1394160	0.820133	8.056995e-53	-8.615850e-47
Msa1354620	Msa1394210	0.829716	4.406417e-55	-8.615850e-47
Msa1354620	Msa1394220	0.822251	2.618302e-53	-8.615850e-47
Msa1354620	Msa1460120	0.842600	2.342862e-58	-8.615850e-47
Msa1354620	Msa1460130	0.851510	8.468226e-61	-8.615850e-47
Msa0604740	Msa1354620	0.805664	1.196568e-49	-8.615850e-47
Msa0139800	Msa1354620	0.800655	1.301925e-48	-8.615850e-47
Msa0202640	Msa1354620	0.827259	1.727140e-54	-8.615850e-47
Msa1305090	Msa1354620	0.858668	7.034498e-63	-8.615850e-47
Msa0370210	Msa1354620	0.829128	6.119813e-55	-8.615850e-47
Msa0398630	Msa1354620	0.804796	1.818771e-49	-8.615850e-47
Msa0405420	Msa1354620	0.840076	1.081082e-57	-8.615850e-47
Msa0405430	Msa1354620	0.876295	1.564219e-68	-8.615850e-47
Msa0405440	Msa1354620	0.857272	1.827175e-62	-8.615850e-47
Msa0405510	Msa1354620	0.851223	1.020750e-60	-8.615850e-47
Msa0405520	Msa1354620	0.853230	2.742139e-61	-8.615850e-47
Msa0405530	Msa1354620	0.853467	2.344900e-61	-8.615850e-47
Msa0983630	Msa1354620	0.906683	1.141907e-80	-8.615850e-47
Msa0983640	Msa1354620	0.850900	1.259116e-60	-8.615850e-47
Msa0983720	Msa1354620	0.800185	1.622741e-48	-8.615850e-47
Msa1022050	Msa1354620	0.887874	9.721502e-73	-8.615850e-47
Msa1022060	Msa1354620	0.846339	2.310985e-59	-8.615850e-47
Msa1064410	Msa1354620	0.840019	1.118446e-57	-8.615850e-47
Msa1086070	Msa1354620	0.811522	6.713014e-51	-8.615850e-47
Msa1086080	Msa1354620	0.838465	2.829845e-57	-8.615850e-47
Msa1086090	Msa1354620	0.823855	1.106098e-53	-8.615850e-47
Msa1086100	Msa1354620	0.816207	6.227278e-52	-8.615850e-47
Msa1086140	Msa1354620	0.832109	1.140303e-55	-8.615850e-47
Msa1086150	Msa1354620	0.812700	3.714724e-51	-8.615850e-47
Msa1086180	Msa1354620	0.816615	5.048788e-52	-8.615850e-47
Msa1086190	Msa1354620	0.841131	5.722981e-58	-8.615850e-47
Msa1086200	Msa1354620	0.826487	2.640187e-54	-8.615850e-47
Msa1086210	Msa1354620	0.832515	9.044927e-56	-8.615850e-47
Msa1086270	Msa1354620	0.821024	5.030358e-53	-8.615850e-47
Msa1086380	Msa1354620	0.812370	4.386502e-51	-8.615850e-47
Msa1086450	Msa1354620	0.812739	3.643889e-51	-8.615850e-47
Msa1086470	Msa1354620	0.807620	4.623814e-50	-8.615850e-47
Msa1086550	Msa1354620	0.844269	8.393862e-59	-8.615850e-47
Msa1086560	Msa1354620	0.835115	2.024615e-56	-8.615850e-47
Msa1086590	Msa1354620	0.870477	1.416719e-66	-8.615850e-47
Msa1086600	Msa1354620	0.875684	2.537323e-68	-8.615850e-47
Msa1086720	Msa1354620	0.863472	2.430456e-64	-8.615850e-47
Msa1086730	Msa1354620	0.909842	3.638154e-82	-8.615850e-47
Msa1086740	Msa1354620	0.857884	1.203992e-62	-8.615850e-47
Msa1086790	Msa1354620	0.899504	1.856813e-77	-8.615850e-47
Msa1086800	Msa1354620	0.864106	1.543315e-64	-8.615850e-47
Msa1086880	Msa1354620	0.823978	1.035409e-53	-8.615850e-47
Msa1086890	Msa1354620	0.827008	1.983438e-54	-8.615850e-47
Msa1086900	Msa1354620	0.815541	8.768529e-52	-8.615850e-47
Msa1086910	Msa1354620	0.816053	6.742076e-52	-8.615850e-47
Msa1086960	Msa1354620	0.890163	1.262284e-73	-8.615850e-47
Msa1086970	Msa1354620	0.856961	2.257728e-62	-8.615850e-47
Msa1087030	Msa1354620	0.859916	2.969540e-63	-8.615850e-47
Msa1087040	Msa1354620	0.891679	3.187476e-74	-8.615850e-47
Msa1087050	Msa1354620	0.853419	2.420126e-61	-8.615850e-47
Msa1087110	Msa1354620	0.895505	8.995551e-76	-8.615850e-47
Msa1087120	Msa1354620	0.852183	5.454768e-61	-8.615850e-47
Msa0263770	Msa1354620	0.838028	3.668327e-57	-8.615850e-47
Msa0268170	Msa1354620	0.808473	3.044007e-50	-8.615850e-47
Msa0268250	Msa1354620	0.866123	3.588675e-65	-8.615850e-47
Msa0268260	Msa1354620	0.917224	6.891549e-86	-8.615850e-47
Msa0268270	Msa1354620	0.869214	3.660291e-66	-8.615850e-47
Msa0268300	Msa1354620	0.867903	9.711376e-66	-8.615850e-47
Msa0268310	Msa1354620	0.872938	2.164048e-67	-8.615850e-47
Msa0268390	Msa1354620	0.817007	4.122094e-52	-8.615850e-47
Msa0268400	Msa1354620	0.808024	3.794837e-50	-8.615850e-47
Msa0268410	Msa1354620	0.822154	2.756346e-53	-8.615850e-47
Msa0268550	Msa1354620	0.823325	1.471639e-53	-8.615850e-47
Msa0268560	Msa1354620	0.842859	1.998373e-58	-8.615850e-47
Msa0268570	Msa1354620	0.806865	6.683767e-50	-8.615850e-47
Msa0268580	Msa1354620	0.829869	4.043022e-55	-8.615850e-47
Msa0268630	Msa1354620	0.833231	6.004847e-56	-8.615850e-47
Msa0268640	Msa1354620	0.820105	8.176248e-53	-8.615850e-47
Msa0268740	Msa1354620	0.835114	2.026272e-56	-8.615850e-47
Msa0268750	Msa1354620	0.822094	2.846343e-53	-8.615850e-47
Msa0268760	Msa1354620	0.825375	4.851692e-54	-8.615850e-47
Msa0268830	Msa1354620	0.850205	1.974272e-60	-8.615850e-47
Msa0268840	Msa1354620	0.809738	1.630950e-50	-8.615850e-47
Msa0268940	Msa1354620	0.800350	1.501907e-48	-8.615850e-47
Msa0268950	Msa1354620	0.838892	2.195240e-57	-8.615850e-47
Msa0268960	Msa1354620	0.840718	7.342948e-58	-8.615850e-47
Msa0269040	Msa1354620	0.850262	1.902526e-60	-8.615850e-47
Msa0269050	Msa1354620	0.826523	2.589223e-54	-8.615850e-47
Msa0269130	Msa1354620	0.847748	9.499848e-60	-8.615850e-47
Msa0269140	Msa1354620	0.924390	7.510367e-90	-8.615850e-47
Msa0269150	Msa1354620	0.878446	2.791278e-69	-8.615850e-47
Msa0344180	Msa1354620	0.808183	3.510513e-50	-8.615850e-47
Msa0344190	Msa1354620	0.831484	1.626142e-55	-8.615850e-47
Msa0344310	Msa1354620	0.849386	3.342637e-60	-8.615850e-47
Msa1143120	Msa1354620	0.835281	1.838427e-56	-8.615850e-47
Msa1143160	Msa1354620	0.831145	1.970597e-55	-8.615850e-47
Msa1143170	Msa1354620	0.811584	6.508674e-51	-8.615850e-47
Msa1143180	Msa1354620	0.819901	9.105020e-53	-8.615850e-47
Msa0582510	Msa1354620	0.844211	8.700143e-59	-8.615850e-47
Msa0582520	Msa1354620	0.812308	4.524848e-51	-8.615850e-47
Msa0582630	Msa1354620	0.826413	2.749813e-54	-8.615850e-47
Msa0582640	Msa1354620	0.860839	1.560697e-63	-8.615850e-47
Msa0582650	Msa1354620	0.804340	2.263681e-49	-8.615850e-47
Msa0582660	Msa1354620	0.826506	2.613835e-54	-8.615850e-47
Msa0582690	Msa1354620	0.846771	1.761875e-59	-8.615850e-47
Msa0582700	Msa1354620	0.818085	2.356669e-52	-8.615850e-47
Msa0582750	Msa1354620	0.821276	4.400435e-53	-8.615850e-47
Msa0582760	Msa1354620	0.847186	1.356111e-59	-8.615850e-47
Msa0582770	Msa1354620	0.801953	7.058415e-49	-8.615850e-47
Msa0582780	Msa1354620	0.827777	1.296907e-54	-8.615850e-47
Msa0582810	Msa1354620	0.843591	1.274931e-58	-8.615850e-47
Msa0582820	Msa1354620	0.819238	1.289684e-52	-8.615850e-47
Msa0582910	Msa1354620	0.834512	2.870972e-56	-8.615850e-47
Msa0582920	Msa1354620	0.816254	6.081001e-52	-8.615850e-47
Msa0582930	Msa1354620	0.832039	1.186617e-55	-8.615850e-47
Msa0591270	Msa1354620	0.800191	1.617757e-48	-8.615850e-47
Msa0591290	Msa1354620	0.852465	4.534627e-61	-8.615850e-47
Msa0591310	Msa1354620	0.859893	3.017914e-63	-8.615850e-47
Msa0591350	Msa1354620	0.896691	2.891936e-76	-8.615850e-47
Msa0591360	Msa1354620	0.834981	2.187949e-56	-8.615850e-47
Msa0591370	Msa1354620	0.801684	8.017091e-49	-8.615850e-47
Msa0591380	Msa1354620	0.823671	1.221631e-53	-8.615850e-47
Msa0867680	Msa1354620	0.903847	2.272101e-79	-8.615850e-47
Msa0867690	Msa1354620	0.857030	2.154560e-62	-8.615850e-47
Msa0876520	Msa1354620	0.803404	3.543539e-49	-8.615850e-47
Msa0908220	Msa1354620	0.863786	1.941051e-64	-8.615850e-47
Msa0908230	Msa1354620	0.915044	9.391405e-85	-8.615850e-47
Msa0908240	Msa1354620	0.864131	1.516557e-64	-8.615850e-47
Msa0908310	Msa1354620	0.900651	5.919408e-78	-8.615850e-47
Msa0908320	Msa1354620	0.849216	3.728469e-60	-8.615850e-47
Msa0908370	Msa1354620	0.893292	7.202043e-75	-8.615850e-47
Msa0908380	Msa1354620	0.839239	1.784142e-57	-8.615850e-47
Msa0908430	Msa1354620	0.846865	1.660501e-59	-8.615850e-47
Msa0908440	Msa1354620	0.944038	4.050619e-103	-8.615850e-47
Msa0908450	Msa1354620	0.877862	4.471017e-69	-8.615850e-47
Msa0908480	Msa1354620	0.823157	1.611493e-53	-8.615850e-47
Msa0908530	Msa1354620	0.907897	3.083036e-81	-8.615850e-47
Msa0908540	Msa1354620	0.868321	7.122365e-66	-8.615850e-47
Msa0908590	Msa1354620	0.851569	8.150178e-61	-8.615850e-47
Msa0908600	Msa1354620	0.921926	1.907705e-88	-8.615850e-47
Msa0908610	Msa1354620	0.828965	6.704373e-55	-8.615850e-47
Msa0908650	Msa1354620	0.847640	1.017437e-59	-8.615850e-47
Msa0908660	Msa1354620	0.937598	2.669398e-98	-8.615850e-47
Msa0908670	Msa1354620	0.883005	6.469914e-71	-8.615850e-47
Msa0908720	Msa1354620	0.891575	3.504591e-74	-8.615850e-47
Msa0908730	Msa1354620	0.856014	4.283426e-62	-8.615850e-47
Msa0911190	Msa1354620	0.851641	7.775355e-61	-8.615850e-47
Msa0911200	Msa1354620	0.859250	4.711313e-63	-8.615850e-47
Msa0911230	Msa1354620	0.828519	8.594670e-55	-8.615850e-47
Msa0924830	Msa1354620	0.808282	3.344004e-50	-8.615850e-47
Msa0924870	Msa1354620	0.816308	5.914281e-52	-8.615850e-47
Msa0924890	Msa1354620	0.806731	7.132541e-50	-8.615850e-47
Msa0924930	Msa1354620	0.891924	2.545449e-74	-8.615850e-47
Msa0924940	Msa1354620	0.854110	1.530611e-61	-8.615850e-47
Msa0925100	Msa1354620	0.872401	3.272272e-67	-8.615850e-47
Msa0925110	Msa1354620	0.863953	1.721964e-64	-8.615850e-47
Msa0931070	Msa1354620	0.829180	5.944672e-55	-8.615850e-47
Msa0931080	Msa1354620	0.803167	3.966796e-49	-8.615850e-47
Msa0931130	Msa1354620	0.831118	2.001252e-55	-8.615850e-47
Msa0931140	Msa1354620	0.802680	5.001437e-49	-8.615850e-47
Msa0931220	Msa1354620	0.829758	4.303293e-55	-8.615850e-47
Msa0931230	Msa1354620	0.801021	1.096028e-48	-8.615850e-47
Msa0931240	Msa1354620	0.826306	2.917034e-54	-8.615850e-47
Msa0931260	Msa1354620	0.854420	1.245745e-61	-8.615850e-47
Msa0931270	Msa1354620	0.823072	1.686309e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1354620	MtrunA17_CPg0492421	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1354620	MtrunA17_Chr4g0016101	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1354620	MtrunA17_Chr3g0090841	91.729	133	9	1	1	131	25	157	1.30e-85	246
Msa1354620	MtrunA17_Chr4g0002541	83.333	60	10	0	1	60	1	60	3.60e-30	103
Msa1354620	MtrunA17_Chr4g0057401	87.500	40	5	0	92	131	1	40	1.06e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1354620	ATCG00900.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1354620	ATCG01240.1	95.420	131	6	0	1	131	25	155	7.02e-91	260
Msa1354620	ATMG01270.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5
Msa1354620	AT2G07696.1	33.824	136	80	3	2	131	15	146	3.86e-18	75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCATTCGTTGGTTATT+AGG	0.268444	tig0014543:-401843	Msa1354620:CDS
CATCAAGTTCCCGTTGAAAT+AGG	0.307921	tig0014543:-401885	Msa1354620:CDS
AAAACGTCCAGGTCGAAATA+TGG	0.317789	tig0014543:-401811	Msa1354620:CDS
CAAATTAAGTTCCGAATTAG+TGG	0.342047	tig0014543:-401784	Msa1354620:CDS
TGGCGATGCCATACGCAAAA+AGG	0.366109	tig0014543:-401745	Msa1354620:CDS
TTTGGCAGCATCCACTAATT+CGG	0.404543	tig0014543:+401773	None:intergenic
ACGTATTGCTTGACGTAAAA+CGG	0.405511	tig0014543:+401959	None:intergenic
GCTTGACGTAAAACGGATAG+TGG	0.418362	tig0014543:+401966	None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG	0.427761	tig0014543:-401915	Msa1354620:CDS
TATGGCATCGCCATTCCCTT+TGG	0.436234	tig0014543:+401755	None:intergenic
TGTGTGGATCCTATTTCAAC+GGG	0.470768	tig0014543:+401876	None:intergenic
TTATCTATCGAGCTATGAAA+AGG	0.512086	tig0014543:-402013	Msa1354620:CDS
GAAAAGCACTTGCCATTCGT+TGG	0.526859	tig0014543:-401851	Msa1354620:CDS
GTGGATGCTGCCAAAGGGAA+TGG	0.532446	tig0014543:-401765	Msa1354620:CDS
GGCGCATCCCGAAAACGTCC+AGG	0.540732	tig0014543:-401822	Msa1354620:CDS
AAAGGAAGAGACTCATAGAA+TGG	0.548096	tig0014543:-401727	Msa1354620:CDS
TTGTGTGGATCCTATTTCAA+CGG	0.548545	tig0014543:+401875	None:intergenic
AATTAGTGGATGCTGCCAAA+GGG	0.575951	tig0014543:-401770	Msa1354620:CDS
GAATTAGTGGATGCTGCCAA+AGG	0.584526	tig0014543:-401771	Msa1354620:CDS
AGAGACTCATAGAATGGCAG+AGG	0.593635	tig0014543:-401721	Msa1354620:CDS
TTGAAAGCCATATTTCGACC+TGG	0.605724	tig0014543:+401804	None:intergenic
TGCGCCTAATAACCAACGAA+TGG	0.620213	tig0014543:+401839	None:intergenic
GTTAACCGTATTATGAAACA+CGG	0.650772	tig0014543:-402059	Msa1354620:CDS
TTTACGTCAAGCAATACGTG+GGG	0.715577	tig0014543:-401955	Msa1354620:CDS
GTTGAAATAGGATCCACACA+AGG	0.731975	tig0014543:-401873	Msa1354620:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTTTCCGTGTTTCATAATA+CGG	+	tig0014543:401706-401725	None:intergenic	25.0%
	GTTAACCGTATTATGAAACA+CGG	-	tig0014543:401698-401717	Msa1354620:CDS	30.0%
	GAAACACGGAAAAAAATCAT+TGG	-	tig0014543:401712-401731	Msa1354620:CDS	30.0%
	TTATCTATCGAGCTATGAAA+AGG	-	tig0014543:401744-401763	Msa1354620:CDS	30.0%
!!	TCTTGCTTTTACTGCTATAT+CGG	+	tig0014543:401834-401853	None:intergenic	30.0%
	CAAATTAAGTTCCGAATTAG+TGG	-	tig0014543:401973-401992	Msa1354620:CDS	30.0%
	ACGTATTGCTTGACGTAAAA+CGG	+	tig0014543:401801-401820	None:intergenic	35.0%
!	TTTTACGTCAAGCAATACGT+GGG	-	tig0014543:401801-401820	Msa1354620:CDS	35.0%
!!	CTTGCTTTTACTGCTATATC+GGG	+	tig0014543:401833-401852	None:intergenic	35.0%
	TTGTGTGGATCCTATTTCAA+CGG	+	tig0014543:401885-401904	None:intergenic	35.0%
	AAAGGAAGAGACTCATAGAA+TGG	-	tig0014543:402030-402049	Msa1354620:CDS	35.0%
!	GTTTTACGTCAAGCAATACG+TGG	-	tig0014543:401800-401819	Msa1354620:CDS	40.0%
	TTTACGTCAAGCAATACGTG+GGG	-	tig0014543:401802-401821	Msa1354620:CDS	40.0%
	GTAAAAGCAAGACGCGTAAA+CGG	-	tig0014543:401842-401861	Msa1354620:CDS	40.0%
	CATCAAGTTCCCGTTGAAAT+AGG	-	tig0014543:401872-401891	Msa1354620:CDS	40.0%
	TGTGTGGATCCTATTTCAAC+GGG	+	tig0014543:401884-401903	None:intergenic	40.0%
	GTTGAAATAGGATCCACACA+AGG	-	tig0014543:401884-401903	Msa1354620:CDS	40.0%
!	CTTGCCATTCGTTGGTTATT+AGG	-	tig0014543:401914-401933	Msa1354620:CDS	40.0%
!	TATTTCGACCTGGACGTTTT+CGG	+	tig0014543:401946-401965	None:intergenic	40.0%
	AAAACGTCCAGGTCGAAATA+TGG	-	tig0014543:401946-401965	Msa1354620:CDS	40.0%
	TTGAAAGCCATATTTCGACC+TGG	+	tig0014543:401956-401975	None:intergenic	40.0%
	TTTGGCAGCATCCACTAATT+CGG	+	tig0014543:401987-402006	None:intergenic	40.0%
!	AATTAGTGGATGCTGCCAAA+GGG	-	tig0014543:401987-402006	Msa1354620:CDS	40.0%
!	AGTCTCTTCCTTTTTGCGTA+TGG	+	tig0014543:402023-402042	None:intergenic	40.0%
	GCTTGACGTAAAACGGATAG+TGG	+	tig0014543:401794-401813	None:intergenic	45.0%
!!	GAAAAGCACTTGCCATTCGT+TGG	-	tig0014543:401906-401925	Msa1354620:CDS	45.0%
	TGCGCCTAATAACCAACGAA+TGG	+	tig0014543:401921-401940	None:intergenic	45.0%
!	ATTTCGACCTGGACGTTTTC+GGG	+	tig0014543:401945-401964	None:intergenic	45.0%
!	GAATTAGTGGATGCTGCCAA+AGG	-	tig0014543:401986-402005	Msa1354620:CDS	45.0%
	AGAGACTCATAGAATGGCAG+AGG	-	tig0014543:402036-402055	Msa1354620:CDS	45.0%
!	GGCAAGTGCTTTTCCTTGTG+TGG	+	tig0014543:401900-401919	None:intergenic	50.0%
	TATGGCATCGCCATTCCCTT+TGG	+	tig0014543:402005-402024	None:intergenic	50.0%
	TGGCGATGCCATACGCAAAA+AGG	-	tig0014543:402012-402031	Msa1354620:CDS	50.0%
	GTGGATGCTGCCAAAGGGAA+TGG	-	tig0014543:401992-402011	Msa1354620:CDS	55.0%
	GGCGCATCCCGAAAACGTCC+AGG	-	tig0014543:401935-401954	Msa1354620:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0014543	gene	401692	402087	401692	ID=Msa1354620;Name=Msa1354620
tig0014543	mRNA	401692	402087	401692	ID=Msa1354620-mRNA-1;Parent=Msa1354620;Name=Msa1354620-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|131
tig0014543	exon	401692	402087	401692	ID=Msa1354620-mRNA-1:exon:9189;Parent=Msa1354620-mRNA-1
tig0014543	CDS	401692	402087	401692	ID=Msa1354620-mRNA-1:cds;Parent=Msa1354620-mRNA-1

Gene Sequence

>Msa1354620

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1354620

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*