Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0908230 | YP_009141653.1 | 100.000 | 155 | 0 | 0 | 1 | 155 | 1 | 155 | 3.73e-109 | 317 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0908230 | sp|Q9B1A1|RR7_LOTJA | 96.774 | 155 | 5 | 0 | 1 | 155 | 1 | 155 | 3.68e-109 | 310 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0202640 | Msa0908230 | 0.822214 | 2.669860e-53 | -8.615850e-47 |
| Msa0370210 | Msa0908230 | 0.815603 | 8.494664e-52 | -8.615850e-47 |
| Msa0405420 | Msa0908230 | 0.848210 | 7.087289e-60 | -8.615850e-47 |
| Msa0405430 | Msa0908230 | 0.890499 | 9.321991e-74 | -8.615850e-47 |
| Msa0405440 | Msa0908230 | 0.868467 | 6.391719e-66 | -8.615850e-47 |
| Msa0405510 | Msa0908230 | 0.863094 | 3.181442e-64 | -8.615850e-47 |
| Msa0405520 | Msa0908230 | 0.869259 | 3.539467e-66 | -8.615850e-47 |
| Msa0405530 | Msa0908230 | 0.858888 | 6.047424e-63 | -8.615850e-47 |
| Msa0263770 | Msa0908230 | 0.844666 | 6.564221e-59 | -8.615850e-47 |
| Msa0268170 | Msa0908230 | 0.804801 | 1.814232e-49 | -8.615850e-47 |
| Msa0268250 | Msa0908230 | 0.835419 | 1.696738e-56 | -8.615850e-47 |
| Msa0268260 | Msa0908230 | 0.912877 | 1.176196e-83 | -8.615850e-47 |
| Msa0268270 | Msa0908230 | 0.871865 | 4.933672e-67 | -8.615850e-47 |
| Msa0268300 | Msa0908230 | 0.890272 | 1.144910e-73 | -8.615850e-47 |
| Msa0268310 | Msa0908230 | 0.865265 | 6.691702e-65 | -8.615850e-47 |
| Msa0268390 | Msa0908230 | 0.805806 | 1.117367e-49 | -8.615850e-47 |
| Msa0268400 | Msa0908230 | 0.805336 | 1.402482e-49 | -8.615850e-47 |
| Msa0268410 | Msa0908230 | 0.815494 | 8.984563e-52 | -8.615850e-47 |
| Msa0268550 | Msa0908230 | 0.809702 | 1.660672e-50 | -8.615850e-47 |
| Msa0268560 | Msa0908230 | 0.817339 | 3.471370e-52 | -8.615850e-47 |
| Msa0268570 | Msa0908230 | 0.813204 | 2.881446e-51 | -8.615850e-47 |
| Msa0268580 | Msa0908230 | 0.816937 | 4.273163e-52 | -8.615850e-47 |
| Msa0268630 | Msa0908230 | 0.818700 | 1.709423e-52 | -8.615850e-47 |
| Msa0268640 | Msa0908230 | 0.800479 | 1.413469e-48 | -8.615850e-47 |
| Msa0268740 | Msa0908230 | 0.821381 | 4.162640e-53 | -8.615850e-47 |
| Msa0268750 | Msa0908230 | 0.819899 | 9.116187e-53 | -8.615850e-47 |
| Msa0268830 | Msa0908230 | 0.835857 | 1.314428e-56 | -8.615850e-47 |
| Msa0268840 | Msa0908230 | 0.805003 | 1.646228e-49 | -8.615850e-47 |
| Msa0268940 | Msa0908230 | 0.800855 | 1.184685e-48 | -8.615850e-47 |
| Msa0268950 | Msa0908230 | 0.815635 | 8.358114e-52 | -8.615850e-47 |
| Msa0268960 | Msa0908230 | 0.833543 | 5.019979e-56 | -8.615850e-47 |
| Msa0269040 | Msa0908230 | 0.844433 | 7.585379e-59 | -8.615850e-47 |
| Msa0269050 | Msa0908230 | 0.816714 | 4.795485e-52 | -8.615850e-47 |
| Msa0269130 | Msa0908230 | 0.845085 | 5.059443e-59 | -8.615850e-47 |
| Msa0269140 | Msa0908230 | 0.920683 | 9.381091e-88 | -8.615850e-47 |
| Msa0269150 | Msa0908230 | 0.880077 | 7.384406e-70 | -8.615850e-47 |
| Msa0344180 | Msa0908230 | 0.801947 | 7.080753e-49 | -8.615850e-47 |
| Msa0344190 | Msa0908230 | 0.803891 | 2.806873e-49 | -8.615850e-47 |
| Msa0344310 | Msa0908230 | 0.839523 | 1.505608e-57 | -8.615850e-47 |
| Msa0582510 | Msa0908230 | 0.839677 | 1.372962e-57 | -8.615850e-47 |
| Msa0582630 | Msa0908230 | 0.816776 | 4.645052e-52 | -8.615850e-47 |
| Msa0582640 | Msa0908230 | 0.832082 | 1.158033e-55 | -8.615850e-47 |
| Msa0582650 | Msa0908230 | 0.818213 | 2.203734e-52 | -8.615850e-47 |
| Msa0582660 | Msa0908230 | 0.820572 | 6.389046e-53 | -8.615850e-47 |
| Msa0582690 | Msa0908230 | 0.843870 | 1.074211e-58 | -8.615850e-47 |
| Msa0582700 | Msa0908230 | 0.804589 | 2.008721e-49 | -8.615850e-47 |
| Msa0582750 | Msa0908230 | 0.818136 | 2.293869e-52 | -8.615850e-47 |
| Msa0582760 | Msa0908230 | 0.828734 | 7.624378e-55 | -8.615850e-47 |
| Msa0582770 | Msa0908230 | 0.805697 | 1.177950e-49 | -8.615850e-47 |
| Msa0582780 | Msa0908230 | 0.825529 | 4.460939e-54 | -8.615850e-47 |
| Msa0582810 | Msa0908230 | 0.843235 | 1.587651e-58 | -8.615850e-47 |
| Msa0582820 | Msa0908230 | 0.813785 | 2.147297e-51 | -8.615850e-47 |
| Msa0582910 | Msa0908230 | 0.836224 | 1.060976e-56 | -8.615850e-47 |
| Msa0582920 | Msa0908230 | 0.808861 | 2.515095e-50 | -8.615850e-47 |
| Msa0582930 | Msa0908230 | 0.814990 | 1.162687e-51 | -8.615850e-47 |
| Msa0591290 | Msa0908230 | 0.859411 | 4.213463e-63 | -8.615850e-47 |
| Msa0591310 | Msa0908230 | 0.849459 | 3.190038e-60 | -8.615850e-47 |
| Msa0591350 | Msa0908230 | 0.900139 | 9.871165e-78 | -8.615850e-47 |
| Msa0591360 | Msa0908230 | 0.829332 | 5.462561e-55 | -8.615850e-47 |
| Msa0591380 | Msa0908230 | 0.823720 | 1.190169e-53 | -8.615850e-47 |
| Msa0867680 | Msa0908230 | 0.903919 | 2.106546e-79 | -8.615850e-47 |
| Msa0867690 | Msa0908230 | 0.834965 | 2.208562e-56 | -8.615850e-47 |
| Msa0908220 | Msa0908230 | 0.855623 | 5.571008e-62 | -8.615850e-47 |
| Msa0908230 | Msa0908240 | 0.875388 | 3.205869e-68 | -8.615850e-47 |
| Msa0908230 | Msa0908310 | 0.897978 | 8.316862e-77 | -8.615850e-47 |
| Msa0908230 | Msa0908320 | 0.842177 | 3.032412e-58 | -8.615850e-47 |
| Msa0908230 | Msa0908370 | 0.885705 | 6.459290e-72 | -8.615850e-47 |
| Msa0908230 | Msa0908380 | 0.830968 | 2.178217e-55 | -8.615850e-47 |
| Msa0908230 | Msa0908430 | 0.846894 | 1.630699e-59 | -8.615850e-47 |
| Msa0908230 | Msa0908440 | 0.943807 | 6.169854e-103 | -8.615850e-47 |
| Msa0908230 | Msa0908450 | 0.888726 | 4.572986e-73 | -8.615850e-47 |
| Msa0908230 | Msa0908480 | 0.836256 | 1.041106e-56 | -8.615850e-47 |
| Msa0908230 | Msa0908530 | 0.907725 | 3.713749e-81 | -8.615850e-47 |
| Msa0908230 | Msa0908540 | 0.866000 | 3.924473e-65 | -8.615850e-47 |
| Msa0908230 | Msa0908590 | 0.844014 | 9.824802e-59 | -8.615850e-47 |
| Msa0908230 | Msa0908600 | 0.914537 | 1.706034e-84 | -8.615850e-47 |
| Msa0908230 | Msa0908610 | 0.843167 | 1.655011e-58 | -8.615850e-47 |
| Msa0908230 | Msa0908650 | 0.846742 | 1.793850e-59 | -8.615850e-47 |
| Msa0908230 | Msa0908660 | 0.927598 | 9.401364e-92 | -8.615850e-47 |
| Msa0908230 | Msa0908670 | 0.878935 | 1.877397e-69 | -8.615850e-47 |
| Msa0908230 | Msa0908720 | 0.899818 | 1.359353e-77 | -8.615850e-47 |
| Msa0908230 | Msa0908730 | 0.844622 | 6.743816e-59 | -8.615850e-47 |
| Msa0908230 | Msa0911190 | 0.865254 | 6.747740e-65 | -8.615850e-47 |
| Msa0908230 | Msa0911200 | 0.823562 | 1.295732e-53 | -8.615850e-47 |
| Msa0908230 | Msa0911230 | 0.815579 | 8.599904e-52 | -8.615850e-47 |
| Msa0908230 | Msa0924830 | 0.826252 | 3.004737e-54 | -8.615850e-47 |
| Msa0908230 | Msa0924880 | 0.800212 | 1.602343e-48 | -8.615850e-47 |
| Msa0908230 | Msa0924930 | 0.878394 | 2.910432e-69 | -8.615850e-47 |
| Msa0908230 | Msa0924940 | 0.835307 | 1.811097e-56 | -8.615850e-47 |
| Msa0908230 | Msa0925100 | 0.888157 | 7.571570e-73 | -8.615850e-47 |
| Msa0908230 | Msa0925110 | 0.858064 | 1.064462e-62 | -8.615850e-47 |
| Msa0908230 | Msa0931070 | 0.821175 | 4.642188e-53 | -8.615850e-47 |
| Msa0908230 | Msa0931130 | 0.833403 | 5.442600e-56 | -8.615850e-47 |
| Msa0908230 | Msa0931230 | 0.800448 | 1.434282e-48 | -8.615850e-47 |
| Msa0908230 | Msa0931240 | 0.800772 | 1.231998e-48 | -8.615850e-47 |
| Msa0908230 | Msa0931260 | 0.843942 | 1.027270e-58 | -8.615850e-47 |
| Msa0908230 | Msa0983630 | 0.897690 | 1.100973e-76 | -8.615850e-47 |
| Msa0908230 | Msa0983640 | 0.831477 | 1.632353e-55 | -8.615850e-47 |
| Msa0908230 | Msa1022050 | 0.883395 | 4.654695e-71 | -8.615850e-47 |
| Msa0908230 | Msa1022060 | 0.836307 | 1.010390e-56 | -8.615850e-47 |
| Msa0908230 | Msa1064410 | 0.840498 | 8.388907e-58 | -8.615850e-47 |
| Msa0908230 | Msa1086070 | 0.806061 | 9.877128e-50 | -8.615850e-47 |
| Msa0908230 | Msa1086080 | 0.814119 | 1.811633e-51 | -8.615850e-47 |
| Msa0908230 | Msa1086090 | 0.821240 | 4.486001e-53 | -8.615850e-47 |
| Msa0908230 | Msa1086100 | 0.809735 | 1.633457e-50 | -8.615850e-47 |
| Msa0908230 | Msa1086140 | 0.829284 | 5.611846e-55 | -8.615850e-47 |
| Msa0908230 | Msa1086180 | 0.807157 | 5.798176e-50 | -8.615850e-47 |
| Msa0908230 | Msa1086190 | 0.820665 | 6.083064e-53 | -8.615850e-47 |
| Msa0908230 | Msa1086200 | 0.824961 | 6.077614e-54 | -8.615850e-47 |
| Msa0908230 | Msa1086210 | 0.811716 | 6.092604e-51 | -8.615850e-47 |
| Msa0908230 | Msa1086250 | 0.804444 | 2.153797e-49 | -8.615850e-47 |
| Msa0908230 | Msa1086270 | 0.828127 | 1.068174e-54 | -8.615850e-47 |
| Msa0908230 | Msa1086280 | 0.800461 | 1.425633e-48 | -8.615850e-47 |
| Msa0908230 | Msa1086380 | 0.801984 | 6.956374e-49 | -8.615850e-47 |
| Msa0908230 | Msa1086550 | 0.846179 | 2.554792e-59 | -8.615850e-47 |
| Msa0908230 | Msa1086560 | 0.835924 | 1.264094e-56 | -8.615850e-47 |
| Msa0908230 | Msa1086590 | 0.888504 | 5.568489e-73 | -8.615850e-47 |
| Msa0908230 | Msa1086600 | 0.876382 | 1.459613e-68 | -8.615850e-47 |
| Msa0908230 | Msa1086720 | 0.845595 | 3.682375e-59 | -8.615850e-47 |
| Msa0908230 | Msa1086730 | 0.933856 | 9.908312e-96 | -8.615850e-47 |
| Msa0908230 | Msa1086740 | 0.879538 | 1.148776e-69 | -8.615850e-47 |
| Msa0908230 | Msa1086790 | 0.901774 | 1.905418e-78 | -8.615850e-47 |
| Msa0908230 | Msa1086800 | 0.876345 | 1.503457e-68 | -8.615850e-47 |
| Msa0908230 | Msa1086880 | 0.806807 | 6.876549e-50 | -8.615850e-47 |
| Msa0908230 | Msa1086890 | 0.810958 | 8.898353e-51 | -8.615850e-47 |
| Msa0908230 | Msa1086900 | 0.820837 | 5.555306e-53 | -8.615850e-47 |
| Msa0908230 | Msa1086910 | 0.804123 | 2.512466e-49 | -8.615850e-47 |
| Msa0908230 | Msa1086960 | 0.892450 | 1.570277e-74 | -8.615850e-47 |
| Msa0908230 | Msa1086970 | 0.847680 | 9.922560e-60 | -8.615850e-47 |
| Msa0908230 | Msa1087030 | 0.846675 | 1.871359e-59 | -8.615850e-47 |
| Msa0908230 | Msa1087040 | 0.924133 | 1.057550e-89 | -8.615850e-47 |
| Msa0908230 | Msa1087050 | 0.878882 | 1.958818e-69 | -8.615850e-47 |
| Msa0908230 | Msa1087110 | 0.899298 | 2.277287e-77 | -8.615850e-47 |
| Msa0908230 | Msa1087120 | 0.851117 | 1.093811e-60 | -8.615850e-47 |
| Msa0908230 | Msa1143120 | 0.842673 | 2.239508e-58 | -8.615850e-47 |
| Msa0908230 | Msa1143160 | 0.801029 | 1.091955e-48 | -8.615850e-47 |
| Msa0908230 | Msa1143170 | 0.823267 | 1.518910e-53 | -8.615850e-47 |
| Msa0908230 | Msa1143180 | 0.807905 | 4.021743e-50 | -8.615850e-47 |
| Msa0908230 | Msa1305090 | 0.828375 | 9.312936e-55 | -8.615850e-47 |
| Msa0908230 | Msa1354320 | 0.800234 | 1.585802e-48 | -8.615850e-47 |
| Msa0908230 | Msa1354420 | 0.903577 | 3.004568e-79 | -8.615850e-47 |
| Msa0908230 | Msa1354430 | 0.858860 | 6.164688e-63 | -8.615850e-47 |
| Msa0908230 | Msa1354480 | 0.848186 | 7.194522e-60 | -8.615850e-47 |
| Msa0908230 | Msa1354490 | 0.932169 | 1.274123e-94 | -8.615850e-47 |
| Msa0908230 | Msa1354500 | 0.878674 | 2.319155e-69 | -8.615850e-47 |
| Msa0908230 | Msa1354550 | 0.909500 | 5.317559e-82 | -8.615850e-47 |
| Msa0908230 | Msa1354560 | 0.853618 | 2.120887e-61 | -8.615850e-47 |
| Msa0908230 | Msa1354610 | 0.867565 | 1.245929e-65 | -8.615850e-47 |
| Msa0908230 | Msa1354620 | 0.915044 | 9.391405e-85 | -8.615850e-47 |
| Msa0908230 | Msa1354630 | 0.890927 | 6.323371e-74 | -8.615850e-47 |
| Msa0908230 | Msa1354680 | 0.865399 | 6.074610e-65 | -8.615850e-47 |
| Msa0908230 | Msa1354690 | 0.926020 | 8.310705e-91 | -8.615850e-47 |
| Msa0908230 | Msa1354700 | 0.843952 | 1.020626e-58 | -8.615850e-47 |
| Msa0908230 | Msa1354740 | 0.853885 | 1.777313e-61 | -8.615850e-47 |
| Msa0908230 | Msa1354750 | 0.925750 | 1.200347e-90 | -8.615850e-47 |
| Msa0908230 | Msa1354760 | 0.872408 | 3.254002e-67 | -8.615850e-47 |
| Msa0908230 | Msa1354810 | 0.904227 | 1.529843e-79 | -8.615850e-47 |
| Msa0908230 | Msa1354820 | 0.874601 | 5.944720e-68 | -8.615850e-47 |
| Msa0908230 | Msa1354910 | 0.822335 | 2.502545e-53 | -8.615850e-47 |
| Msa0908230 | Msa1354920 | 0.832483 | 9.214533e-56 | -8.615850e-47 |
| Msa0908230 | Msa1354930 | 0.808824 | 2.561741e-50 | -8.615850e-47 |
| Msa0908230 | Msa1354980 | 0.897546 | 1.266407e-76 | -8.615850e-47 |
| Msa0908230 | Msa1354990 | 0.846523 | 2.059149e-59 | -8.615850e-47 |
| Msa0908230 | Msa1355080 | 0.808880 | 2.491941e-50 | -8.615850e-47 |
| Msa0908230 | Msa1355090 | 0.837572 | 4.803416e-57 | -8.615850e-47 |
| Msa0908230 | Msa1355100 | 0.803131 | 4.034782e-49 | -8.615850e-47 |
| Msa0908230 | Msa1365890 | 0.890999 | 5.923823e-74 | -8.615850e-47 |
| Msa0908230 | Msa1365900 | 0.849286 | 3.563620e-60 | -8.615850e-47 |
| Msa0908230 | Msa1365950 | 0.856057 | 4.159885e-62 | -8.615850e-47 |
| Msa0908230 | Msa1365960 | 0.877625 | 5.409184e-69 | -8.615850e-47 |
| Msa0908230 | Msa1365970 | 0.854041 | 1.602704e-61 | -8.615850e-47 |
| Msa0908230 | Msa1366080 | 0.896265 | 4.352935e-76 | -8.615850e-47 |
| Msa0908230 | Msa1366090 | 0.852195 | 5.414668e-61 | -8.615850e-47 |
| Msa0908230 | Msa1368230 | 0.899197 | 2.513990e-77 | -8.615850e-47 |
| Msa0908230 | Msa1368240 | 0.851685 | 7.552715e-61 | -8.615850e-47 |
| Msa0908230 | Msa1380600 | 0.806095 | 9.715774e-50 | -8.615850e-47 |
| Msa0908230 | Msa1380620 | 0.801843 | 7.435110e-49 | -8.615850e-47 |
| Msa0908230 | Msa1385410 | 0.886571 | 3.044916e-72 | -8.615850e-47 |
| Msa0908230 | Msa1385420 | 0.831271 | 1.835379e-55 | -8.615850e-47 |
| Msa0908230 | Msa1391090 | 0.858333 | 8.855163e-63 | -8.615850e-47 |
| Msa0908230 | Msa1391100 | 0.907505 | 4.714395e-81 | -8.615850e-47 |
| Msa0908230 | Msa1391110 | 0.868122 | 8.254241e-66 | -8.615850e-47 |
| Msa0908230 | Msa1391160 | 0.906749 | 1.063983e-80 | -8.615850e-47 |
| Msa0908230 | Msa1391170 | 0.828137 | 1.062365e-54 | -8.615850e-47 |
| Msa0908230 | Msa1391220 | 0.861153 | 1.252825e-63 | -8.615850e-47 |
| Msa0908230 | Msa1391230 | 0.890466 | 9.601994e-74 | -8.615850e-47 |
| Msa0908230 | Msa1391240 | 0.868406 | 6.685722e-66 | -8.615850e-47 |
| Msa0908230 | Msa1394030 | 0.828554 | 8.428539e-55 | -8.615850e-47 |
| Msa0908230 | Msa1394040 | 0.830560 | 2.742348e-55 | -8.615850e-47 |
| Msa0908230 | Msa1394150 | 0.815784 | 7.742346e-52 | -8.615850e-47 |
| Msa0908230 | Msa1394160 | 0.821848 | 3.246671e-53 | -8.615850e-47 |
| Msa0908230 | Msa1394210 | 0.820853 | 5.506215e-53 | -8.615850e-47 |
| Msa0908230 | Msa1460120 | 0.863638 | 2.157419e-64 | -8.615850e-47 |
| Msa0908230 | Msa1460130 | 0.865461 | 5.808901e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0908230 | MtrunA17_CPg0492421 | 92.994 | 157 | 9 | 1 | 1 | 155 | 1 | 157 | 3.39e-104 | 294 |
| Msa0908230 | MtrunA17_Chr4g0016101 | 92.994 | 157 | 9 | 1 | 1 | 155 | 1 | 157 | 3.39e-104 | 294 |
| Msa0908230 | MtrunA17_Chr3g0090841 | 92.994 | 157 | 9 | 1 | 1 | 155 | 1 | 157 | 3.39e-104 | 294 |
| Msa0908230 | MtrunA17_Chr4g0002541 | 83.333 | 60 | 10 | 0 | 25 | 84 | 1 | 60 | 7.63e-30 | 103 |
| Msa0908230 | MtrunA17_Chr4g0057401 | 87.500 | 40 | 5 | 0 | 116 | 155 | 1 | 40 | 2.03e-17 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0908230 | ATCG00900.1 | 95.484 | 155 | 7 | 0 | 1 | 155 | 1 | 155 | 3.40e-109 | 307 |
| Msa0908230 | ATCG01240.1 | 95.484 | 155 | 7 | 0 | 1 | 155 | 1 | 155 | 3.40e-109 | 307 |
| Msa0908230 | ATMG01270.1 | 33.571 | 140 | 83 | 3 | 22 | 155 | 11 | 146 | 1.72e-18 | 77.4 |
| Msa0908230 | AT2G07696.1 | 33.571 | 140 | 83 | 3 | 22 | 155 | 11 | 146 | 1.72e-18 | 77.4 |
Find 29 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAATCGATTACGATAAAT+TGG | 0.238141 | 6_2:+9504132 | None:intergenic |
| CTTGCCATTCGTTGGTTATT+AGG | 0.268444 | 6_2:-9503883 | Msa0908230:CDS |
| CATCAAGTTCCCGTTGAAAT+AGG | 0.307921 | 6_2:-9503925 | Msa0908230:CDS |
| AAAACGTCCAGGTCGAAATA+TGG | 0.317789 | 6_2:-9503851 | Msa0908230:CDS |
| CAAATTAAGTTCCGAATTAG+TGG | 0.342047 | 6_2:-9503824 | Msa0908230:CDS |
| TGGCGATGCCATACGCAAAA+AGG | 0.366109 | 6_2:-9503785 | Msa0908230:CDS |
| TTTGGCAGCATCCACTAATT+CGG | 0.404543 | 6_2:+9503813 | None:intergenic |
| ACGTATTGCTTGACGTAAAA+CGG | 0.405511 | 6_2:+9503999 | None:intergenic |
| GCTTGACGTAAAACGGATAG+TGG | 0.418362 | 6_2:+9504006 | None:intergenic |
| GTAAAAGCAAGACGCGTAAA+CGG | 0.427761 | 6_2:-9503955 | Msa0908230:CDS |
| TATGGCATCGCCATTCCCTT+TGG | 0.436234 | 6_2:+9503795 | None:intergenic |
| TGTGTGGATCCTATTTCAAC+GGG | 0.470768 | 6_2:+9503916 | None:intergenic |
| TTATCTATCGAGCTATGAAA+AGG | 0.512086 | 6_2:-9504053 | Msa0908230:CDS |
| GAAAAGCACTTGCCATTCGT+TGG | 0.526859 | 6_2:-9503891 | Msa0908230:CDS |
| GTGGATGCTGCCAAAGGGAA+TGG | 0.532446 | 6_2:-9503805 | Msa0908230:CDS |
| TAATCGATTAGTTAACATGT+TGG | 0.538486 | 6_2:-9504121 | Msa0908230:CDS |
| GGCGCATCCCGAAAACGTCC+AGG | 0.540732 | 6_2:-9503862 | Msa0908230:CDS |
| AAAGGAAGAGACTCATAGAA+TGG | 0.548096 | 6_2:-9503767 | Msa0908230:CDS |
| TTGTGTGGATCCTATTTCAA+CGG | 0.548545 | 6_2:+9503915 | None:intergenic |
| AATTAGTGGATGCTGCCAAA+GGG | 0.575951 | 6_2:-9503810 | Msa0908230:CDS |
| GAATTAGTGGATGCTGCCAA+AGG | 0.584526 | 6_2:-9503811 | Msa0908230:CDS |
| AGAGACTCATAGAATGGCAG+AGG | 0.593635 | 6_2:-9503761 | Msa0908230:CDS |
| TTGAAAGCCATATTTCGACC+TGG | 0.605724 | 6_2:+9503844 | None:intergenic |
| TGCGCCTAATAACCAACGAA+TGG | 0.620213 | 6_2:+9503879 | None:intergenic |
| TCGATTACGATAAATTGGAT+CGG | 0.627987 | 6_2:+9504137 | None:intergenic |
| GTTAACCGTATTATGAAACA+CGG | 0.650772 | 6_2:-9504099 | Msa0908230:CDS |
| TTTACGCTCATGTCACGGCG+AGG | 0.672550 | 6_2:-9504186 | None:intergenic |
| TTTACGTCAAGCAATACGTG+GGG | 0.715577 | 6_2:-9503995 | Msa0908230:CDS |
| GTTGAAATAGGATCCACACA+AGG | 0.731975 | 6_2:-9503913 | Msa0908230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTAATCGATTACGATAAAT+TGG | + | chr6_2:9503780-9503799 | None:intergenic | 25.0% |
| !! | TAATCGATTAGTTAACATGT+TGG | - | chr6_2:9503788-9503807 | Msa0908230:CDS | 25.0% |
| !! | TTTTTCCGTGTTTCATAATA+CGG | + | chr6_2:9503818-9503837 | None:intergenic | 25.0% |
| ! | ACTAATCGATTACGATAAAT+TGG | + | chr6_2:9503780-9503799 | None:intergenic | 25.0% |
| !! | TAATCGATTAGTTAACATGT+TGG | - | chr6_2:9503788-9503807 | Msa0908230:CDS | 25.0% |
| !! | TTTTTCCGTGTTTCATAATA+CGG | + | chr6_2:9503818-9503837 | None:intergenic | 25.0% |
| ! | ATAAATTGGATCGGATTTTG+CGG | + | chr6_2:9503766-9503785 | None:intergenic | 30.0% |
| TCGATTACGATAAATTGGAT+CGG | + | chr6_2:9503775-9503794 | None:intergenic | 30.0% | |
| GTTAACCGTATTATGAAACA+CGG | - | chr6_2:9503810-9503829 | Msa0908230:CDS | 30.0% | |
| GAAACACGGAAAAAAATCAT+TGG | - | chr6_2:9503824-9503843 | Msa0908230:CDS | 30.0% | |
| TTATCTATCGAGCTATGAAA+AGG | - | chr6_2:9503856-9503875 | Msa0908230:CDS | 30.0% | |
| !! | TCTTGCTTTTACTGCTATAT+CGG | + | chr6_2:9503946-9503965 | None:intergenic | 30.0% |
| CAAATTAAGTTCCGAATTAG+TGG | - | chr6_2:9504085-9504104 | Msa0908230:CDS | 30.0% | |
| ! | ATAAATTGGATCGGATTTTG+CGG | + | chr6_2:9503766-9503785 | None:intergenic | 30.0% |
| TCGATTACGATAAATTGGAT+CGG | + | chr6_2:9503775-9503794 | None:intergenic | 30.0% | |
| GTTAACCGTATTATGAAACA+CGG | - | chr6_2:9503810-9503829 | Msa0908230:CDS | 30.0% | |
| GAAACACGGAAAAAAATCAT+TGG | - | chr6_2:9503824-9503843 | Msa0908230:CDS | 30.0% | |
| TTATCTATCGAGCTATGAAA+AGG | - | chr6_2:9503856-9503875 | Msa0908230:CDS | 30.0% | |
| !! | TCTTGCTTTTACTGCTATAT+CGG | + | chr6_2:9503946-9503965 | None:intergenic | 30.0% |
| CAAATTAAGTTCCGAATTAG+TGG | - | chr6_2:9504085-9504104 | Msa0908230:CDS | 30.0% | |
| ACGTATTGCTTGACGTAAAA+CGG | + | chr6_2:9503913-9503932 | None:intergenic | 35.0% | |
| ! | TTTTACGTCAAGCAATACGT+GGG | - | chr6_2:9503913-9503932 | Msa0908230:CDS | 35.0% |
| !! | CTTGCTTTTACTGCTATATC+GGG | + | chr6_2:9503945-9503964 | None:intergenic | 35.0% |
| TTGTGTGGATCCTATTTCAA+CGG | + | chr6_2:9503997-9504016 | None:intergenic | 35.0% | |
| AAAGGAAGAGACTCATAGAA+TGG | - | chr6_2:9504142-9504161 | Msa0908230:CDS | 35.0% | |
| ACGTATTGCTTGACGTAAAA+CGG | + | chr6_2:9503913-9503932 | None:intergenic | 35.0% | |
| ! | TTTTACGTCAAGCAATACGT+GGG | - | chr6_2:9503913-9503932 | Msa0908230:CDS | 35.0% |
| !! | CTTGCTTTTACTGCTATATC+GGG | + | chr6_2:9503945-9503964 | None:intergenic | 35.0% |
| TTGTGTGGATCCTATTTCAA+CGG | + | chr6_2:9503997-9504016 | None:intergenic | 35.0% | |
| AAAGGAAGAGACTCATAGAA+TGG | - | chr6_2:9504142-9504161 | Msa0908230:CDS | 35.0% | |
| ! | GTTTTACGTCAAGCAATACG+TGG | - | chr6_2:9503912-9503931 | Msa0908230:CDS | 40.0% |
| TTTACGTCAAGCAATACGTG+GGG | - | chr6_2:9503914-9503933 | Msa0908230:CDS | 40.0% | |
| GTAAAAGCAAGACGCGTAAA+CGG | - | chr6_2:9503954-9503973 | Msa0908230:CDS | 40.0% | |
| CATCAAGTTCCCGTTGAAAT+AGG | - | chr6_2:9503984-9504003 | Msa0908230:CDS | 40.0% | |
| TGTGTGGATCCTATTTCAAC+GGG | + | chr6_2:9503996-9504015 | None:intergenic | 40.0% | |
| GTTGAAATAGGATCCACACA+AGG | - | chr6_2:9503996-9504015 | Msa0908230:CDS | 40.0% | |
| ! | CTTGCCATTCGTTGGTTATT+AGG | - | chr6_2:9504026-9504045 | Msa0908230:CDS | 40.0% |
| ! | TATTTCGACCTGGACGTTTT+CGG | + | chr6_2:9504058-9504077 | None:intergenic | 40.0% |
| AAAACGTCCAGGTCGAAATA+TGG | - | chr6_2:9504058-9504077 | Msa0908230:CDS | 40.0% | |
| TTGAAAGCCATATTTCGACC+TGG | + | chr6_2:9504068-9504087 | None:intergenic | 40.0% | |
| TTTGGCAGCATCCACTAATT+CGG | + | chr6_2:9504099-9504118 | None:intergenic | 40.0% | |
| ! | AATTAGTGGATGCTGCCAAA+GGG | - | chr6_2:9504099-9504118 | Msa0908230:CDS | 40.0% |
| ! | AGTCTCTTCCTTTTTGCGTA+TGG | + | chr6_2:9504135-9504154 | None:intergenic | 40.0% |
| ! | GTTTTACGTCAAGCAATACG+TGG | - | chr6_2:9503912-9503931 | Msa0908230:CDS | 40.0% |
| TTTACGTCAAGCAATACGTG+GGG | - | chr6_2:9503914-9503933 | Msa0908230:CDS | 40.0% | |
| GTAAAAGCAAGACGCGTAAA+CGG | - | chr6_2:9503954-9503973 | Msa0908230:CDS | 40.0% | |
| CATCAAGTTCCCGTTGAAAT+AGG | - | chr6_2:9503984-9504003 | Msa0908230:CDS | 40.0% | |
| TGTGTGGATCCTATTTCAAC+GGG | + | chr6_2:9503996-9504015 | None:intergenic | 40.0% | |
| GTTGAAATAGGATCCACACA+AGG | - | chr6_2:9503996-9504015 | Msa0908230:CDS | 40.0% | |
| ! | CTTGCCATTCGTTGGTTATT+AGG | - | chr6_2:9504026-9504045 | Msa0908230:CDS | 40.0% |
| ! | TATTTCGACCTGGACGTTTT+CGG | + | chr6_2:9504058-9504077 | None:intergenic | 40.0% |
| AAAACGTCCAGGTCGAAATA+TGG | - | chr6_2:9504058-9504077 | Msa0908230:CDS | 40.0% | |
| TTGAAAGCCATATTTCGACC+TGG | + | chr6_2:9504068-9504087 | None:intergenic | 40.0% | |
| TTTGGCAGCATCCACTAATT+CGG | + | chr6_2:9504099-9504118 | None:intergenic | 40.0% | |
| ! | AATTAGTGGATGCTGCCAAA+GGG | - | chr6_2:9504099-9504118 | Msa0908230:CDS | 40.0% |
| ! | AGTCTCTTCCTTTTTGCGTA+TGG | + | chr6_2:9504135-9504154 | None:intergenic | 40.0% |
| GCTTGACGTAAAACGGATAG+TGG | + | chr6_2:9503906-9503925 | None:intergenic | 45.0% | |
| !! | GAAAAGCACTTGCCATTCGT+TGG | - | chr6_2:9504018-9504037 | Msa0908230:CDS | 45.0% |
| TGCGCCTAATAACCAACGAA+TGG | + | chr6_2:9504033-9504052 | None:intergenic | 45.0% | |
| ! | ATTTCGACCTGGACGTTTTC+GGG | + | chr6_2:9504057-9504076 | None:intergenic | 45.0% |
| ! | GAATTAGTGGATGCTGCCAA+AGG | - | chr6_2:9504098-9504117 | Msa0908230:CDS | 45.0% |
| AGAGACTCATAGAATGGCAG+AGG | - | chr6_2:9504148-9504167 | Msa0908230:CDS | 45.0% | |
| GCTTGACGTAAAACGGATAG+TGG | + | chr6_2:9503906-9503925 | None:intergenic | 45.0% | |
| !! | GAAAAGCACTTGCCATTCGT+TGG | - | chr6_2:9504018-9504037 | Msa0908230:CDS | 45.0% |
| TGCGCCTAATAACCAACGAA+TGG | + | chr6_2:9504033-9504052 | None:intergenic | 45.0% | |
| ! | ATTTCGACCTGGACGTTTTC+GGG | + | chr6_2:9504057-9504076 | None:intergenic | 45.0% |
| ! | GAATTAGTGGATGCTGCCAA+AGG | - | chr6_2:9504098-9504117 | Msa0908230:CDS | 45.0% |
| AGAGACTCATAGAATGGCAG+AGG | - | chr6_2:9504148-9504167 | Msa0908230:CDS | 45.0% | |
| ! | GGCAAGTGCTTTTCCTTGTG+TGG | + | chr6_2:9504012-9504031 | None:intergenic | 50.0% |
| TATGGCATCGCCATTCCCTT+TGG | + | chr6_2:9504117-9504136 | None:intergenic | 50.0% | |
| TGGCGATGCCATACGCAAAA+AGG | - | chr6_2:9504124-9504143 | Msa0908230:CDS | 50.0% | |
| ! | GGCAAGTGCTTTTCCTTGTG+TGG | + | chr6_2:9504012-9504031 | None:intergenic | 50.0% |
| TATGGCATCGCCATTCCCTT+TGG | + | chr6_2:9504117-9504136 | None:intergenic | 50.0% | |
| TGGCGATGCCATACGCAAAA+AGG | - | chr6_2:9504124-9504143 | Msa0908230:CDS | 50.0% | |
| GTGGATGCTGCCAAAGGGAA+TGG | - | chr6_2:9504104-9504123 | Msa0908230:CDS | 55.0% | |
| GTGGATGCTGCCAAAGGGAA+TGG | - | chr6_2:9504104-9504123 | Msa0908230:CDS | 55.0% | |
| GGCGCATCCCGAAAACGTCC+AGG | - | chr6_2:9504047-9504066 | Msa0908230:CDS | 65.0% | |
| GGCGCATCCCGAAAACGTCC+AGG | - | chr6_2:9504047-9504066 | Msa0908230:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 9503732 | 9504199 | 9503732 | ID=Msa0908230;Name=Msa0908230 |
| chr6_2 | mRNA | 9503732 | 9504199 | 9503732 | ID=Msa0908230-mRNA-1;Parent=Msa0908230;Name=Msa0908230-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|155 |
| chr6_2 | exon | 9503732 | 9504199 | 9503732 | ID=Msa0908230-mRNA-1:exon:2292;Parent=Msa0908230-mRNA-1 |
| chr6_2 | CDS | 9503732 | 9504199 | 9503732 | ID=Msa0908230-mRNA-1:cds;Parent=Msa0908230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
