AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0908600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908600 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0908600 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0908600 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0908600 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa0908600 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0908600 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0908600 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0908600 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa0908600 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 6_2:-9754801 Msa0908600:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 6_2:-9754843 Msa0908600:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 6_2:-9754769 Msa0908600:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 6_2:-9754742 Msa0908600:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 6_2:-9754703 Msa0908600:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 6_2:+9754731 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 6_2:+9754917 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 6_2:+9754924 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 6_2:-9754873 Msa0908600:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 6_2:+9754713 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 6_2:+9754834 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 6_2:-9754971 Msa0908600:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 6_2:-9754809 Msa0908600:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 6_2:-9754723 Msa0908600:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 6_2:-9754780 Msa0908600:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 6_2:-9754685 Msa0908600:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 6_2:+9754833 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 6_2:-9754728 Msa0908600:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 6_2:-9754729 Msa0908600:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 6_2:-9754679 Msa0908600:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 6_2:+9754762 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 6_2:+9754797 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 6_2:-9755017 Msa0908600:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 6_2:-9754913 Msa0908600:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 6_2:-9754831 Msa0908600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + chr6_2:9754664-9754683 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - chr6_2:9754656-9754675 Msa0908600:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - chr6_2:9754670-9754689 Msa0908600:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - chr6_2:9754702-9754721 Msa0908600:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + chr6_2:9754792-9754811 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - chr6_2:9754931-9754950 Msa0908600:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + chr6_2:9754759-9754778 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - chr6_2:9754759-9754778 Msa0908600:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + chr6_2:9754791-9754810 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + chr6_2:9754843-9754862 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - chr6_2:9754988-9755007 Msa0908600:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - chr6_2:9754758-9754777 Msa0908600:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - chr6_2:9754760-9754779 Msa0908600:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - chr6_2:9754800-9754819 Msa0908600:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - chr6_2:9754830-9754849 Msa0908600:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + chr6_2:9754842-9754861 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - chr6_2:9754842-9754861 Msa0908600:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - chr6_2:9754872-9754891 Msa0908600:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + chr6_2:9754904-9754923 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - chr6_2:9754904-9754923 Msa0908600:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + chr6_2:9754914-9754933 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + chr6_2:9754945-9754964 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - chr6_2:9754945-9754964 Msa0908600:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + chr6_2:9754981-9755000 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + chr6_2:9754752-9754771 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - chr6_2:9754864-9754883 Msa0908600:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + chr6_2:9754879-9754898 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + chr6_2:9754903-9754922 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - chr6_2:9754944-9754963 Msa0908600:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - chr6_2:9754994-9755013 Msa0908600:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + chr6_2:9754858-9754877 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + chr6_2:9754963-9754982 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - chr6_2:9754970-9754989 Msa0908600:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - chr6_2:9754950-9754969 Msa0908600:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - chr6_2:9754893-9754912 Msa0908600:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 9754650 9755045 9754650 ID=Msa0908600;Name=Msa0908600
chr6_2 mRNA 9754650 9755045 9754650 ID=Msa0908600-mRNA-1;Parent=Msa0908600;Name=Msa0908600-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
chr6_2 exon 9754650 9755045 9754650 ID=Msa0908600-mRNA-1:exon:2377;Parent=Msa0908600-mRNA-1
chr6_2 CDS 9754650 9755045 9754650 ID=Msa0908600-mRNA-1:cds;Parent=Msa0908600-mRNA-1
Gene Sequence

>Msa0908600

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa0908600

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*