AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354490 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1354490 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1354490 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa1354490 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa1354490 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354490 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1354490 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa1354490 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa1354490 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 tig0014543:-301744 Msa1354490:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 tig0014543:-301786 Msa1354490:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 tig0014543:-301712 Msa1354490:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 tig0014543:-301685 Msa1354490:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 tig0014543:-301646 Msa1354490:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 tig0014543:+301674 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 tig0014543:+301860 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 tig0014543:+301867 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 tig0014543:-301816 Msa1354490:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 tig0014543:+301656 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 tig0014543:+301777 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 tig0014543:-301914 Msa1354490:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 tig0014543:-301752 Msa1354490:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 tig0014543:-301666 Msa1354490:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 tig0014543:-301723 Msa1354490:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 tig0014543:-301628 Msa1354490:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 tig0014543:+301776 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 tig0014543:-301671 Msa1354490:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 tig0014543:-301672 Msa1354490:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 tig0014543:-301622 Msa1354490:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 tig0014543:+301705 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 tig0014543:+301740 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 tig0014543:-301960 Msa1354490:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 tig0014543:-301856 Msa1354490:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 tig0014543:-301774 Msa1354490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + tig0014543:301607-301626 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - tig0014543:301599-301618 Msa1354490:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - tig0014543:301613-301632 Msa1354490:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - tig0014543:301645-301664 Msa1354490:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + tig0014543:301735-301754 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - tig0014543:301874-301893 Msa1354490:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + tig0014543:301702-301721 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - tig0014543:301702-301721 Msa1354490:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + tig0014543:301734-301753 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + tig0014543:301786-301805 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - tig0014543:301931-301950 Msa1354490:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - tig0014543:301701-301720 Msa1354490:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - tig0014543:301703-301722 Msa1354490:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - tig0014543:301743-301762 Msa1354490:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - tig0014543:301773-301792 Msa1354490:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + tig0014543:301785-301804 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - tig0014543:301785-301804 Msa1354490:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - tig0014543:301815-301834 Msa1354490:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + tig0014543:301847-301866 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - tig0014543:301847-301866 Msa1354490:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + tig0014543:301857-301876 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + tig0014543:301888-301907 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - tig0014543:301888-301907 Msa1354490:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + tig0014543:301924-301943 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + tig0014543:301695-301714 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - tig0014543:301807-301826 Msa1354490:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + tig0014543:301822-301841 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + tig0014543:301846-301865 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - tig0014543:301887-301906 Msa1354490:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - tig0014543:301937-301956 Msa1354490:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + tig0014543:301801-301820 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + tig0014543:301906-301925 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - tig0014543:301913-301932 Msa1354490:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - tig0014543:301893-301912 Msa1354490:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - tig0014543:301836-301855 Msa1354490:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 301593 301988 301593 ID=Msa1354490;Name=Msa1354490
tig0014543 mRNA 301593 301988 301593 ID=Msa1354490-mRNA-1;Parent=Msa1354490;Name=Msa1354490-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
tig0014543 exon 301593 301988 301593 ID=Msa1354490-mRNA-1:exon:9138;Parent=Msa1354490-mRNA-1
tig0014543 CDS 301593 301988 301593 ID=Msa1354490-mRNA-1:cds;Parent=Msa1354490-mRNA-1
Gene Sequence

>Msa1354490

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa1354490

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*