AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0268260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268260 MtrunA17_CPg0492421 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0268260 MtrunA17_Chr4g0016101 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0268260 MtrunA17_Chr3g0090841 91.729 133 9 1 1 131 25 157 1.30e-85 246
Msa0268260 MtrunA17_Chr4g0002541 83.333 60 10 0 1 60 1 60 3.60e-30 103
Msa0268260 MtrunA17_Chr4g0057401 87.500 40 5 0 92 131 1 40 1.06e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0268260 ATCG00900.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0268260 ATCG01240.1 95.420 131 6 0 1 131 25 155 7.02e-91 260
Msa0268260 ATMG01270.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5
Msa0268260 AT2G07696.1 33.824 136 80 3 2 131 15 146 3.86e-18 75.5

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCATTCGTTGGTTATT+AGG 0.268444 2_2:-70159847 Msa0268260:CDS
CATCAAGTTCCCGTTGAAAT+AGG 0.307921 2_2:-70159889 Msa0268260:CDS
AAAACGTCCAGGTCGAAATA+TGG 0.317789 2_2:-70159815 Msa0268260:CDS
CAAATTAAGTTCCGAATTAG+TGG 0.342047 2_2:-70159788 Msa0268260:CDS
TGGCGATGCCATACGCAAAA+AGG 0.366109 2_2:-70159749 Msa0268260:CDS
TTTGGCAGCATCCACTAATT+CGG 0.404543 2_2:+70159777 None:intergenic
ACGTATTGCTTGACGTAAAA+CGG 0.405511 2_2:+70159963 None:intergenic
GCTTGACGTAAAACGGATAG+TGG 0.418362 2_2:+70159970 None:intergenic
GTAAAAGCAAGACGCGTAAA+CGG 0.427761 2_2:-70159919 Msa0268260:CDS
TATGGCATCGCCATTCCCTT+TGG 0.436234 2_2:+70159759 None:intergenic
TGTGTGGATCCTATTTCAAC+GGG 0.470768 2_2:+70159880 None:intergenic
TTATCTATCGAGCTATGAAA+AGG 0.512086 2_2:-70160017 Msa0268260:CDS
GAAAAGCACTTGCCATTCGT+TGG 0.526859 2_2:-70159855 Msa0268260:CDS
GTGGATGCTGCCAAAGGGAA+TGG 0.532446 2_2:-70159769 Msa0268260:CDS
GGCGCATCCCGAAAACGTCC+AGG 0.540732 2_2:-70159826 Msa0268260:CDS
AAAGGAAGAGACTCATAGAA+TGG 0.548096 2_2:-70159731 Msa0268260:CDS
TTGTGTGGATCCTATTTCAA+CGG 0.548545 2_2:+70159879 None:intergenic
AATTAGTGGATGCTGCCAAA+GGG 0.575951 2_2:-70159774 Msa0268260:CDS
GAATTAGTGGATGCTGCCAA+AGG 0.584526 2_2:-70159775 Msa0268260:CDS
AGAGACTCATAGAATGGCAG+AGG 0.593635 2_2:-70159725 Msa0268260:CDS
TTGAAAGCCATATTTCGACC+TGG 0.605724 2_2:+70159808 None:intergenic
TGCGCCTAATAACCAACGAA+TGG 0.620213 2_2:+70159843 None:intergenic
GTTAACCGTATTATGAAACA+CGG 0.650772 2_2:-70160063 Msa0268260:CDS
TTTACGTCAAGCAATACGTG+GGG 0.715577 2_2:-70159959 Msa0268260:CDS
GTTGAAATAGGATCCACACA+AGG 0.731975 2_2:-70159877 Msa0268260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTTTCCGTGTTTCATAATA+CGG + chr2_2:70159710-70159729 None:intergenic 25.0%
GTTAACCGTATTATGAAACA+CGG - chr2_2:70159702-70159721 Msa0268260:CDS 30.0%
GAAACACGGAAAAAAATCAT+TGG - chr2_2:70159716-70159735 Msa0268260:CDS 30.0%
TTATCTATCGAGCTATGAAA+AGG - chr2_2:70159748-70159767 Msa0268260:CDS 30.0%
!! TCTTGCTTTTACTGCTATAT+CGG + chr2_2:70159838-70159857 None:intergenic 30.0%
CAAATTAAGTTCCGAATTAG+TGG - chr2_2:70159977-70159996 Msa0268260:CDS 30.0%
ACGTATTGCTTGACGTAAAA+CGG + chr2_2:70159805-70159824 None:intergenic 35.0%
! TTTTACGTCAAGCAATACGT+GGG - chr2_2:70159805-70159824 Msa0268260:CDS 35.0%
!! CTTGCTTTTACTGCTATATC+GGG + chr2_2:70159837-70159856 None:intergenic 35.0%
TTGTGTGGATCCTATTTCAA+CGG + chr2_2:70159889-70159908 None:intergenic 35.0%
AAAGGAAGAGACTCATAGAA+TGG - chr2_2:70160034-70160053 Msa0268260:CDS 35.0%
! GTTTTACGTCAAGCAATACG+TGG - chr2_2:70159804-70159823 Msa0268260:CDS 40.0%
TTTACGTCAAGCAATACGTG+GGG - chr2_2:70159806-70159825 Msa0268260:CDS 40.0%
GTAAAAGCAAGACGCGTAAA+CGG - chr2_2:70159846-70159865 Msa0268260:CDS 40.0%
CATCAAGTTCCCGTTGAAAT+AGG - chr2_2:70159876-70159895 Msa0268260:CDS 40.0%
TGTGTGGATCCTATTTCAAC+GGG + chr2_2:70159888-70159907 None:intergenic 40.0%
GTTGAAATAGGATCCACACA+AGG - chr2_2:70159888-70159907 Msa0268260:CDS 40.0%
! CTTGCCATTCGTTGGTTATT+AGG - chr2_2:70159918-70159937 Msa0268260:CDS 40.0%
! TATTTCGACCTGGACGTTTT+CGG + chr2_2:70159950-70159969 None:intergenic 40.0%
AAAACGTCCAGGTCGAAATA+TGG - chr2_2:70159950-70159969 Msa0268260:CDS 40.0%
TTGAAAGCCATATTTCGACC+TGG + chr2_2:70159960-70159979 None:intergenic 40.0%
TTTGGCAGCATCCACTAATT+CGG + chr2_2:70159991-70160010 None:intergenic 40.0%
! AATTAGTGGATGCTGCCAAA+GGG - chr2_2:70159991-70160010 Msa0268260:CDS 40.0%
! AGTCTCTTCCTTTTTGCGTA+TGG + chr2_2:70160027-70160046 None:intergenic 40.0%
GCTTGACGTAAAACGGATAG+TGG + chr2_2:70159798-70159817 None:intergenic 45.0%
!! GAAAAGCACTTGCCATTCGT+TGG - chr2_2:70159910-70159929 Msa0268260:CDS 45.0%
TGCGCCTAATAACCAACGAA+TGG + chr2_2:70159925-70159944 None:intergenic 45.0%
! ATTTCGACCTGGACGTTTTC+GGG + chr2_2:70159949-70159968 None:intergenic 45.0%
! GAATTAGTGGATGCTGCCAA+AGG - chr2_2:70159990-70160009 Msa0268260:CDS 45.0%
AGAGACTCATAGAATGGCAG+AGG - chr2_2:70160040-70160059 Msa0268260:CDS 45.0%
! GGCAAGTGCTTTTCCTTGTG+TGG + chr2_2:70159904-70159923 None:intergenic 50.0%
TATGGCATCGCCATTCCCTT+TGG + chr2_2:70160009-70160028 None:intergenic 50.0%
TGGCGATGCCATACGCAAAA+AGG - chr2_2:70160016-70160035 Msa0268260:CDS 50.0%
GTGGATGCTGCCAAAGGGAA+TGG - chr2_2:70159996-70160015 Msa0268260:CDS 55.0%
GGCGCATCCCGAAAACGTCC+AGG - chr2_2:70159939-70159958 Msa0268260:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 70159696 70160091 70159696 ID=Msa0268260;Name=Msa0268260
chr2_2 mRNA 70159696 70160091 70159696 ID=Msa0268260-mRNA-1;Parent=Msa0268260;Name=Msa0268260-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|131
chr2_2 exon 70159696 70160091 70159696 ID=Msa0268260-mRNA-1:exon:22483;Parent=Msa0268260-mRNA-1
chr2_2 CDS 70159696 70160091 70159696 ID=Msa0268260-mRNA-1:cds;Parent=Msa0268260-mRNA-1
Gene Sequence

>Msa0268260

ATGTTGGTTAACCGTATTATGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGAGCTATGAAAAGGATTCAACAAAAGACAGAAACAAATCCACTATCCGTTTTACGTCAAGCAATACGTGGGGTAACTCCCGATATAGCAGTAAAAGCAAGACGCGTAAACGGATCGACTCATCAAGTTCCCGTTGAAATAGGATCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGGCGCATCCCGAAAACGTCCAGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAATGGCGATGCCATACGCAAAAAGGAAGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA

Protein sequence

>Msa0268260

MLVNRIMKHGKKSLAYQIIYRAMKRIQQKTETNPLSVLRQAIRGVTPDIAVKARRVNGSTHQVPVEIGSTQGKALAIRWLLGASRKRPGRNMAFKLSSELVDAAKGNGDAIRKKEETHRMAEANRAFAHFR*