Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303820 | XP_027356244.1 | 72.727 | 110 | 27 | 1 | 1 | 110 | 1 | 107 | 3.76e-49 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303820 | sp|Q9LUV2|POP3_ARATH | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.56e-44 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303820 | A0A151TDN5 | 67.857 | 112 | 33 | 1 | 1 | 112 | 1 | 109 | 3.47e-48 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004340 | Msa1303820 | 0.837266 | 5.753291e-57 | -8.615850e-47 |
| Msa0012380 | Msa1303820 | 0.819364 | 1.207303e-52 | -8.615850e-47 |
| Msa0037270 | Msa1303820 | 0.800390 | 1.473842e-48 | -8.615850e-47 |
| Msa0072390 | Msa1303820 | 0.814485 | 1.504540e-51 | -8.615850e-47 |
| Msa0080810 | Msa1303820 | 0.843100 | 1.724360e-58 | -8.615850e-47 |
| Msa0081120 | Msa1303820 | 0.816996 | 4.146429e-52 | -8.615850e-47 |
| Msa0091470 | Msa1303820 | 0.804211 | 2.408494e-49 | -8.615850e-47 |
| Msa0114700 | Msa1303820 | 0.800698 | 1.275706e-48 | -8.615850e-47 |
| Msa0116470 | Msa1303820 | 0.821535 | 3.835430e-53 | -8.615850e-47 |
| Msa0116510 | Msa1303820 | 0.836983 | 6.793557e-57 | -8.615850e-47 |
| Msa0120320 | Msa1303820 | 0.807043 | 6.128698e-50 | -8.615850e-47 |
| Msa0608780 | Msa1303820 | 0.813524 | 2.450215e-51 | -8.615850e-47 |
| Msa0610780 | Msa1303820 | 0.863011 | 3.373764e-64 | -8.615850e-47 |
| Msa0610810 | Msa1303820 | 0.834620 | 2.698083e-56 | -8.615850e-47 |
| Msa0619390 | Msa1303820 | 0.808682 | 2.747410e-50 | -8.615850e-47 |
| Msa0645410 | Msa1303820 | 0.807955 | 3.925330e-50 | -8.615850e-47 |
| Msa0646190 | Msa1303820 | 0.801787 | 7.634695e-49 | -8.615850e-47 |
| Msa0649250 | Msa1303820 | 0.808629 | 2.820018e-50 | -8.615850e-47 |
| Msa0651310 | Msa1303820 | 0.850197 | 1.984014e-60 | -8.615850e-47 |
| Msa0693400 | Msa1303820 | 0.817029 | 4.076475e-52 | -8.615850e-47 |
| Msa0693500 | Msa1303820 | 0.841848 | 3.704768e-58 | -8.615850e-47 |
| Msa0705810 | Msa1303820 | 0.812699 | 3.717991e-51 | -8.615850e-47 |
| Msa0742090 | Msa1303820 | 0.815668 | 8.215419e-52 | -8.615850e-47 |
| Msa0749150 | Msa1303820 | 0.804265 | 2.347098e-49 | -8.615850e-47 |
| Msa0803530 | Msa1303820 | 0.857839 | 1.241997e-62 | -8.615850e-47 |
| Msa0823310 | Msa1303820 | 0.825471 | 4.602808e-54 | -8.615850e-47 |
| Msa0147540 | Msa1303820 | 0.806260 | 8.967853e-50 | -8.615850e-47 |
| Msa0163960 | Msa1303820 | 0.810656 | 1.034166e-50 | -8.615850e-47 |
| Msa0173370 | Msa1303820 | 0.830891 | 2.275110e-55 | -8.615850e-47 |
| Msa0180100 | Msa1303820 | 0.837290 | 5.673301e-57 | -8.615850e-47 |
| Msa0184510 | Msa1303820 | 0.853018 | 3.152100e-61 | -8.615850e-47 |
| Msa0187980 | Msa1303820 | 0.860331 | 2.225933e-63 | -8.615850e-47 |
| Msa0207680 | Msa1303820 | -0.810662 | 1.031074e-50 | -8.615850e-47 |
| Msa1228460 | Msa1303820 | 0.802717 | 4.913206e-49 | -8.615850e-47 |
| Msa1236860 | Msa1303820 | 0.869903 | 2.183523e-66 | -8.615850e-47 |
| Msa1255020 | Msa1303820 | 0.865267 | 6.683330e-65 | -8.615850e-47 |
| Msa1295470 | Msa1303820 | 0.865630 | 5.135340e-65 | -8.615850e-47 |
| Msa1297220 | Msa1303820 | 0.809054 | 2.287366e-50 | -8.615850e-47 |
| Msa1303760 | Msa1303820 | 0.976656 | 3.037957e-142 | -8.615850e-47 |
| Msa1303820 | Msa1317240 | 0.862839 | 3.813326e-64 | -8.615850e-47 |
| Msa1303820 | Msa1332690 | 0.810503 | 1.115924e-50 | -8.615850e-47 |
| Msa1303820 | Msa1333210 | -0.811592 | 6.482595e-51 | -8.615850e-47 |
| Msa1303820 | Msa1343450 | 0.819479 | 1.136391e-52 | -8.615850e-47 |
| Msa1303820 | Msa1363070 | 0.964301 | 3.734343e-123 | -8.615850e-47 |
| Msa1303820 | Msa1373390 | 0.802458 | 5.558379e-49 | -8.615850e-47 |
| Msa1303820 | Msa1382750 | 0.821031 | 5.011381e-53 | -8.615850e-47 |
| Msa1303820 | Msa1404690 | 0.808485 | 3.026602e-50 | -8.615850e-47 |
| Msa1303820 | Msa1450780 | 0.832570 | 8.769125e-56 | -8.615850e-47 |
| Msa1303820 | Msa1460530 | 0.800383 | 1.479083e-48 | -8.615850e-47 |
| Msa0378310 | Msa1303820 | 0.814646 | 1.385942e-51 | -8.615850e-47 |
| Msa0404060 | Msa1303820 | 0.838541 | 2.705143e-57 | -8.615850e-47 |
| Msa0404070 | Msa1303820 | 0.830006 | 3.743994e-55 | -8.615850e-47 |
| Msa0425320 | Msa1303820 | 0.824786 | 6.682302e-54 | -8.615850e-47 |
| Msa0428330 | Msa1303820 | 0.804670 | 1.932133e-49 | -8.615850e-47 |
| Msa0436840 | Msa1303820 | -0.805327 | 1.408004e-49 | -8.615850e-47 |
| Msa0468360 | Msa1303820 | 0.816492 | 5.377090e-52 | -8.615850e-47 |
| Msa0471760 | Msa1303820 | 0.808210 | 3.464150e-50 | -8.615850e-47 |
| Msa1004720 | Msa1303820 | 0.800346 | 1.504670e-48 | -8.615850e-47 |
| Msa1031530 | Msa1303820 | 0.800411 | 1.459528e-48 | -8.615850e-47 |
| Msa1071640 | Msa1303820 | 0.835125 | 2.012975e-56 | -8.615850e-47 |
| Msa1117510 | Msa1303820 | 0.837377 | 5.389540e-57 | -8.615850e-47 |
| Msa1161210 | Msa1303820 | 0.827955 | 1.175350e-54 | -8.615850e-47 |
| Msa1173380 | Msa1303820 | 0.812272 | 4.609407e-51 | -8.615850e-47 |
| Msa1209160 | Msa1303820 | 0.970276 | 2.262955e-131 | -8.615850e-47 |
| Msa1219060 | Msa1303820 | 0.816878 | 4.405691e-52 | -8.615850e-47 |
| Msa1219540 | Msa1303820 | -0.809262 | 2.064138e-50 | -8.615850e-47 |
| Msa0508780 | Msa1303820 | 0.820083 | 8.271625e-53 | -8.615850e-47 |
| Msa0515460 | Msa1303820 | 0.809788 | 1.591420e-50 | -8.615850e-47 |
| Msa0530340 | Msa1303820 | 0.807585 | 4.704249e-50 | -8.615850e-47 |
| Msa0556070 | Msa1303820 | 0.833918 | 4.047340e-56 | -8.615850e-47 |
| Msa0570680 | Msa1303820 | 0.830507 | 2.825378e-55 | -8.615850e-47 |
| Msa0577050 | Msa1303820 | 0.833965 | 3.937210e-56 | -8.615850e-47 |
| Msa0864730 | Msa1303820 | 0.803741 | 3.016876e-49 | -8.615850e-47 |
| Msa0864750 | Msa1303820 | 0.805809 | 1.115587e-49 | -8.615850e-47 |
| Msa0879650 | Msa1303820 | 0.860041 | 2.723125e-63 | -8.615850e-47 |
| Msa0887100 | Msa1303820 | 0.883668 | 3.693457e-71 | -8.615850e-47 |
| Msa0892020 | Msa1303820 | 0.834579 | 2.761780e-56 | -8.615850e-47 |
| Msa0909720 | Msa1303820 | 0.818302 | 2.104041e-52 | -8.615850e-47 |
| Msa0913140 | Msa1303820 | 0.863771 | 1.962012e-64 | -8.615850e-47 |
| Msa0928940 | Msa1303820 | 0.820704 | 5.959650e-53 | -8.615850e-47 |
| Msa0930210 | Msa1303820 | -0.811077 | 8.384149e-51 | -8.615850e-47 |
| Msa0950470 | Msa1303820 | 0.852548 | 4.294374e-61 | -8.615850e-47 |
| Msa0950570 | Msa1303820 | 0.855966 | 4.423214e-62 | -8.615850e-47 |
| Msa0956910 | Msa1303820 | 0.848020 | 7.997284e-60 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303820 | MtrunA17_Chr4g0070211 | 75.000 | 72 | 15 | 1 | 8 | 79 | 3 | 71 | 2.03e-30 | 103 |
| Msa1303820 | MtrunA17_Chr2g0321051 | 36.893 | 103 | 60 | 2 | 9 | 111 | 8 | 105 | 1.39e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1303820 | AT3G17210.1 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.60e-45 | 142 |
| Msa1303820 | AT3G17210.2 | 62.500 | 112 | 39 | 1 | 1 | 112 | 1 | 109 | 2.60e-45 | 142 |
| Msa1303820 | AT5G22580.1 | 34.021 | 97 | 59 | 2 | 9 | 105 | 7 | 98 | 8.53e-15 | 65.5 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTCCTCAATCTTCTCT+TGG | 0.329948 | 8_3:+67117960 | None:intergenic |
| CAAAGTGAAAGAGGAGGAAA+TGG | 0.338863 | 8_3:-67118041 | Msa1303820:exon |
| GATTCAGGTAACAAATAATA+AGG | 0.348275 | 8_3:+67117642 | None:intergenic |
| AATGAGTAAATCCTTGATTC+AGG | 0.352373 | 8_3:+67117627 | None:intergenic |
| AGGAGGGAGCAAAGTGAAAG+AGG | 0.406040 | 8_3:-67118050 | Msa1303820:five_prime_UTR |
| TTACCAATGGAATGACTTCA+TGG | 0.420824 | 8_3:+67117901 | None:intergenic |
| ATTATTTGTTACCTGAATCA+AGG | 0.432767 | 8_3:-67117638 | Msa1303820:CDS |
| AAAGTTGCTCGTTCCTTGCT+TGG | 0.439365 | 8_3:-67117531 | Msa1303820:CDS |
| GAGTTCCTCAATCTTCTCTT+GGG | 0.443255 | 8_3:+67117961 | None:intergenic |
| GTAGTGAAGAACATAGTGAT+TGG | 0.457715 | 8_3:-67118004 | Msa1303820:CDS |
| AGTGAAAGAGGAGGAAATGG+AGG | 0.502250 | 8_3:-67118038 | Msa1303820:exon |
| GAGGAAATGGAGGAAGCAAA+AGG | 0.502851 | 8_3:-67118028 | Msa1303820:exon |
| TGGGATGAGATTGACGAGAT+TGG | 0.516286 | 8_3:+67117925 | None:intergenic |
| ATTCAAAGGTTAACGATGGT+GGG | 0.545431 | 8_3:+67117482 | None:intergenic |
| TCCTTGCTTGGAGAAAGTGC+TGG | 0.548785 | 8_3:-67117519 | Msa1303820:CDS |
| CACCCATGAAGTCATTCCAT+TGG | 0.553232 | 8_3:-67117904 | Msa1303820:intron |
| TGAATCAACATTTGAGAGTG+TGG | 0.555452 | 8_3:-67117600 | Msa1303820:CDS |
| TTTGCATACTCAACATGAGC+AGG | 0.558557 | 8_3:+67117551 | None:intergenic |
| GAAGATTGAGGAACTCATCA+AGG | 0.571882 | 8_3:-67117954 | Msa1303820:CDS |
| GCCAGCACTTTCTCCAAGCA+AGG | 0.576667 | 8_3:+67117518 | None:intergenic |
| GATTCAAAGGTTAACGATGG+TGG | 0.578082 | 8_3:+67117481 | None:intergenic |
| GAGATTGACGAGATTGGCAA+AGG | 0.580515 | 8_3:+67117931 | None:intergenic |
| AGGGAGCAAAGTGAAAGAGG+AGG | 0.582719 | 8_3:-67118047 | Msa1303820:five_prime_UTR |
| CAATGGAATGACTTCATGGG+TGG | 0.583790 | 8_3:+67117905 | None:intergenic |
| AATCTCCCAAGAGAAGATTG+AGG | 0.597731 | 8_3:-67117966 | Msa1303820:CDS |
| ATGAGCTACATACTCTGCAA+TGG | 0.614733 | 8_3:+67117574 | None:intergenic |
| TACCAATGGAATGACTTCAT+GGG | 0.631729 | 8_3:+67117902 | None:intergenic |
| AAGAAACACTGCAATGCACG+AGG | 0.694375 | 8_3:-67118101 | None:intergenic |
| AATGGAATGACTTCATGGGT+GGG | 0.706119 | 8_3:+67117906 | None:intergenic |
| ACGGATTCAAAGGTTAACGA+TGG | 0.711712 | 8_3:+67117478 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTTTTTTAAGTTT+TGG | + | chr8_3:67117778-67117797 | None:intergenic | 10.0% |
| !!! | TATACATGTCTTATTTTTTT+AGG | - | chr8_3:67117895-67117914 | Msa1303820:intron | 15.0% |
| !!! | TTGTAGTATACAGCTATTTT+AGG | - | chr8_3:67117507-67117526 | Msa1303820:CDS | 25.0% |
| !! | TTTAAGTTTCATCTTACCAA+TGG | + | chr8_3:67117692-67117711 | None:intergenic | 25.0% |
| ! | GATTCAGGTAACAAATAATA+AGG | + | chr8_3:67117938-67117957 | None:intergenic | 25.0% |
| !! | ATTATTTGTTACCTGAATCA+AGG | - | chr8_3:67117939-67117958 | Msa1303820:CDS | 25.0% |
| !! | TAGTATACAGCTATTTTAGG+AGG | - | chr8_3:67117510-67117529 | Msa1303820:CDS | 30.0% |
| !! | AGTATACAGCTATTTTAGGA+GGG | - | chr8_3:67117511-67117530 | Msa1303820:CDS | 30.0% |
| AATGAGTAAATCCTTGATTC+AGG | + | chr8_3:67117953-67117972 | None:intergenic | 30.0% | |
| GTAGTGAAGAACATAGTGAT+TGG | - | chr8_3:67117573-67117592 | Msa1303820:CDS | 35.0% | |
| TACCAATGGAATGACTTCAT+GGG | + | chr8_3:67117678-67117697 | None:intergenic | 35.0% | |
| TTACCAATGGAATGACTTCA+TGG | + | chr8_3:67117679-67117698 | None:intergenic | 35.0% | |
| !! | ATAGTCTAGACTGCTTTTGT+CGG | + | chr8_3:67117728-67117747 | None:intergenic | 35.0% |
| TGAATCAACATTTGAGAGTG+TGG | - | chr8_3:67117977-67117996 | Msa1303820:CDS | 35.0% | |
| CAAAGTGAAAGAGGAGGAAA+TGG | - | chr8_3:67117536-67117555 | Msa1303820:CDS | 40.0% | |
| AATCTCCCAAGAGAAGATTG+AGG | - | chr8_3:67117611-67117630 | Msa1303820:CDS | 40.0% | |
| GAGTTCCTCAATCTTCTCTT+GGG | + | chr8_3:67117619-67117638 | None:intergenic | 40.0% | |
| TGAGTTCCTCAATCTTCTCT+TGG | + | chr8_3:67117620-67117639 | None:intergenic | 40.0% | |
| GAAGATTGAGGAACTCATCA+AGG | - | chr8_3:67117623-67117642 | Msa1303820:CDS | 40.0% | |
| AATGGAATGACTTCATGGGT+GGG | + | chr8_3:67117674-67117693 | None:intergenic | 40.0% | |
| ATGAGCTACATACTCTGCAA+TGG | + | chr8_3:67118006-67118025 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGAGC+AGG | + | chr8_3:67118029-67118048 | None:intergenic | 40.0% | |
| AGTGAAAGAGGAGGAAATGG+AGG | - | chr8_3:67117539-67117558 | Msa1303820:CDS | 45.0% | |
| GAGGAAATGGAGGAAGCAAA+AGG | - | chr8_3:67117549-67117568 | Msa1303820:CDS | 45.0% | |
| GAGATTGACGAGATTGGCAA+AGG | + | chr8_3:67117649-67117668 | None:intergenic | 45.0% | |
| TGGGATGAGATTGACGAGAT+TGG | + | chr8_3:67117655-67117674 | None:intergenic | 45.0% | |
| CAATGGAATGACTTCATGGG+TGG | + | chr8_3:67117675-67117694 | None:intergenic | 45.0% | |
| CACCCATGAAGTCATTCCAT+TGG | - | chr8_3:67117673-67117692 | Msa1303820:intron | 45.0% | |
| ! | GATTTTCGCTAAGTGGACGA+GGG | + | chr8_3:67117814-67117833 | None:intergenic | 45.0% |
| ! | TACCAGCGATTTTCGCTAAG+TGG | + | chr8_3:67117821-67117840 | None:intergenic | 45.0% |
| AAAGTTGCTCGTTCCTTGCT+TGG | - | chr8_3:67118046-67118065 | Msa1303820:five_prime_UTR | 45.0% | |
| AGGAGGGAGCAAAGTGAAAG+AGG | - | chr8_3:67117527-67117546 | Msa1303820:CDS | 50.0% | |
| AGGGAGCAAAGTGAAAGAGG+AGG | - | chr8_3:67117530-67117549 | Msa1303820:CDS | 50.0% | |
| ! | CGATTTTCGCTAAGTGGACG+AGG | + | chr8_3:67117815-67117834 | None:intergenic | 50.0% |
| GTCCACTTAGCGAAAATCGC+TGG | - | chr8_3:67117816-67117835 | Msa1303820:intron | 50.0% | |
| ! | TCCTTGCTTGGAGAAAGTGC+TGG | - | chr8_3:67118058-67118077 | Msa1303820:five_prime_UTR | 50.0% |
| GCCAGCACTTTCTCCAAGCA+AGG | + | chr8_3:67118062-67118081 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 67117484 | 67118115 | 67117484 | ID=Msa1303820;Name=Msa1303820 |
| chr8_3 | mRNA | 67117484 | 67118115 | 67117484 | ID=Msa1303820-mRNA-1;Parent=Msa1303820;Name=Msa1303820-mRNA-1;_AED=0.36;_eAED=0.37;_QI=71|0|0.5|1|1|1|2|0|112 |
| chr8_3 | exon | 67117484 | 67117682 | 67117484 | ID=Msa1303820-mRNA-1:exon:20532;Parent=Msa1303820-mRNA-1 |
| chr8_3 | exon | 67117905 | 67118115 | 67117905 | ID=Msa1303820-mRNA-1:exon:20531;Parent=Msa1303820-mRNA-1 |
| chr8_3 | five_prime_UTR | 67118045 | 67118115 | 67118045 | ID=Msa1303820-mRNA-1:five_prime_utr;Parent=Msa1303820-mRNA-1 |
| chr8_3 | CDS | 67117905 | 67118044 | 67117905 | ID=Msa1303820-mRNA-1:cds;Parent=Msa1303820-mRNA-1 |
| chr8_3 | CDS | 67117484 | 67117682 | 67117484 | ID=Msa1303820-mRNA-1:cds;Parent=Msa1303820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
