AlfalfaGEDB | Msa1332690

M. sativa cultivar zhongmu-4 / Msa1332690

KEGG: K21362
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657
PFAM: PF00232.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332690	sp\|Q8L6H7\|SFR2_ORYSJ	69.764	592	163	4	42	617	42	633	0.0	865

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332690	A0A072UTJ9	98.418	632	8	1	1	630	1	632	0.0	1294

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003340	Msa1332690	0.804182	2.442168e-49	-8.615850e-47
Msa0004340	Msa1332690	0.821435	4.042923e-53	-8.615850e-47
Msa0007570	Msa1332690	0.821003	5.085119e-53	-8.615850e-47
Msa0007810	Msa1332690	0.819041	1.430006e-52	-8.615850e-47
Msa0009560	Msa1332690	0.828374	9.313357e-55	-8.615850e-47
Msa0012380	Msa1332690	0.851325	9.555037e-61	-8.615850e-47
Msa0013270	Msa1332690	-0.803634	3.173976e-49	-8.615850e-47
Msa0014590	Msa1332690	-0.809603	1.743712e-50	-8.615850e-47
Msa0014970	Msa1332690	0.831119	1.999617e-55	-8.615850e-47
Msa0016380	Msa1332690	0.841890	3.610445e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332690	MtrunA17_Chr4g0068871	98.418	632	8	1	1	630	1	632	0.0	1294
Msa1332690	MtrunA17_Chr4g0043091	25.478	471	260	15	144	534	46	505	1.49e-32	131
Msa1332690	MtrunA17_Chr4g0042171	28.009	432	225	16	180	536	86	506	3.28e-31	127
Msa1332690	MtrunA17_Chr4g0033311	27.913	412	218	13	181	522	103	505	4.53e-31	127
Msa1332690	MtrunA17_Chr4g0033201	28.058	417	227	14	181	530	100	510	4.26e-30	124
Msa1332690	MtrunA17_Chr4g0033191	27.103	428	234	14	181	540	107	524	4.94e-29	121
Msa1332690	MtrunA17_Chr3g0088441	25.870	460	247	16	145	522	57	504	5.71e-29	121
Msa1332690	MtrunA17_Chr4g0074551	26.464	427	230	14	181	535	113	527	6.69e-29	121
Msa1332690	MtrunA17_Chr4g0033321	25.882	425	230	12	181	532	97	509	1.08e-28	120
Msa1332690	MtrunA17_Chr4g0033401	26.024	415	222	12	181	522	98	500	3.35e-28	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332690	AT3G06510.1	65.353	609	187	4	4	609	1	588	0.0	843
Msa1332690	AT3G06510.2	61.897	643	187	5	4	609	1	622	0.0	828
Msa1332690	AT3G60140.1	26.834	477	261	18	157	559	68	530	2.22e-31	129
Msa1332690	AT1G26560.1	25.219	456	247	15	160	532	64	508	2.37e-31	128
Msa1332690	AT3G60120.1	26.606	436	239	12	167	530	63	489	2.43e-31	129
Msa1332690	AT3G60130.3	28.431	408	222	14	181	530	42	437	2.97e-31	127
Msa1332690	AT3G60130.1	28.162	419	220	14	181	530	94	500	6.63e-31	127
Msa1332690	AT3G60130.2	28.162	419	220	14	181	530	42	448	7.49e-31	126
Msa1332690	AT5G44640.1	26.683	416	238	10	181	534	95	505	3.09e-30	125
Msa1332690	AT2G44470.3	27.033	455	244	17	164	542	75	517	4.66e-30	125

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Find 184 sgRNAs with CRISPR-Local

Find 359 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACAGTGGATTATTTCA+TGG	0.355262	8_4:+52619895	Msa1332690:CDS
AAATAACAGAAGAGAACACA+TGG	0.652388	8_4:+52617601	Msa1332690:five_prime_UTR
AAATGGAAGAGAATTGCATT+CGG	0.461325	8_4:+52622634	Msa1332690:three_prime_UTR
AACAGATGGTAAGAAGGGCG+AGG	0.630415	8_4:-52621281	None:intergenic
AACAGTAACACCAGTATCCT+TGG	0.478658	8_4:-52619476	None:intergenic
AACATGCGATATAAGCCATC+AGG	0.498179	8_4:-52620900	None:intergenic
AACTCATCAAATCAAATAGT+TGG	0.480032	8_4:+52620580	Msa1332690:CDS
AACTGCGACAAGTCCAAACT+TGG	0.422766	8_4:-52621231	None:intergenic
AAGGACTGTTACTTGTTTCA+AGG	0.400317	8_4:+52622834	Msa1332690:three_prime_UTR
AAGTAAGCATACAGAAGACA+TGG	0.587256	8_4:-52620226	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTAGAAATGAATGAAAAGG+AGG	+	chr8_4:52617571-52617590	Msa1332690:five_prime_UTR	30.0%
CTAGAAATGAATGAAAAGGA+GGG	+	chr8_4:52617572-52617591	Msa1332690:five_prime_UTR	30.0%
AAATAACAGAAGAGAACACA+TGG	+	chr8_4:52617601-52617620	Msa1332690:five_prime_UTR	30.0%
ATTACCGGTAATGTTGAAAT+CGG	-	chr8_4:52617937-52617956	None:intergenic	30.0%
AAATAGATTAAGGCTCTCTT+TGG	+	chr8_4:52618058-52618077	Msa1332690:intron	30.0%
TAATAAATAAGCTGCTATCC+TGG	+	chr8_4:52618235-52618254	Msa1332690:intron	30.0%
TTTCATAAGTTCTCCAAACA+TGG	+	chr8_4:52618312-52618331	Msa1332690:intron	30.0%
ACAAACAAATAAGCCATGTT+TGG	-	chr8_4:52618328-52618347	None:intergenic	30.0%
ACTTCAAAATCCAATGTTGA+CGG	-	chr8_4:52618727-52618746	None:intergenic	30.0%
GTAAGCTAATATTGTTGTCA+AGG	+	chr8_4:52618770-52618789	Msa1332690:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	52617492	52622932	52617492	ID=Msa1332690;Name=Msa1332690
chr8_4	mRNA	52617492	52622932	52617492	ID=Msa1332690-mRNA-1;Parent=Msa1332690;Name=Msa1332690-mRNA-1;_AED=0.17;_eAED=0.17;_QI=288\|1\|1\|1\|1\|1\|10\|500\|630
chr8_4	exon	52617492	52617951	52617492	ID=Msa1332690-mRNA-1:exon:15861;Parent=Msa1332690-mRNA-1
chr8_4	exon	52618356	52618686	52618356	ID=Msa1332690-mRNA-1:exon:15862;Parent=Msa1332690-mRNA-1
chr8_4	exon	52619426	52619564	52619426	ID=Msa1332690-mRNA-1:exon:15863;Parent=Msa1332690-mRNA-1
chr8_4	exon	52619761	52619927	52619761	ID=Msa1332690-mRNA-1:exon:15864;Parent=Msa1332690-mRNA-1
chr8_4	exon	52620169	52620390	52620169	ID=Msa1332690-mRNA-1:exon:15865;Parent=Msa1332690-mRNA-1
chr8_4	exon	52620579	52620747	52620579	ID=Msa1332690-mRNA-1:exon:15866;Parent=Msa1332690-mRNA-1
chr8_4	exon	52620828	52621061	52620828	ID=Msa1332690-mRNA-1:exon:15867;Parent=Msa1332690-mRNA-1
chr8_4	exon	52621161	52621310	52621161	ID=Msa1332690-mRNA-1:exon:15868;Parent=Msa1332690-mRNA-1
chr8_4	exon	52621396	52621525	52621396	ID=Msa1332690-mRNA-1:exon:15869;Parent=Msa1332690-mRNA-1
chr8_4	exon	52622254	52622932	52622254	ID=Msa1332690-mRNA-1:exon:15870;Parent=Msa1332690-mRNA-1
chr8_4	five_prime_UTR	52617492	52617779	52617492	ID=Msa1332690-mRNA-1:five_prime_utr;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52617780	52617951	52617780	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52618356	52618686	52618356	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52619426	52619564	52619426	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52619761	52619927	52619761	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52620169	52620390	52620169	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52620579	52620747	52620579	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52620828	52621061	52620828	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52621161	52621310	52621161	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52621396	52621525	52621396	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	CDS	52622254	52622432	52622254	ID=Msa1332690-mRNA-1:cds;Parent=Msa1332690-mRNA-1
chr8_4	three_prime_UTR	52622433	52622932	52622433	ID=Msa1332690-mRNA-1:three_prime_utr;Parent=Msa1332690-mRNA-1

Gene Sequence

>Msa1332690

ATGACGATAGTTTCACTCTTCCTAACTGCCACTAAACTCGCCGGAGCTTTAGTCACTCTCACTGTCGCCGCCAATGCATTTTCCTTCTCTCGTTTTCGCAACAAAAATCTTCGCCGTTTCACTTCCCCTATCGATGAATCCTCCGATACTCTCGCCGATTTCAACATTACCGAAGAAGAAAATGGGTTTTTCTTTGGTCTAGCGACAGCACCGGCACATGTTGAGGACAGGCTTGATGATGCTTGGATTCAGTTTGCTGAACAAGAGAGTGTTGCCGGTGCTGAGCAGAAAGTAGATGCTTTGATGGGCTCTGCCACTGCTGATGGTGGGTCTCAACAAGCCATATCATCTGCACAACGAGGGCTTAAGGGTAACAAGAAGTCTCTTAAGGTAGCTATGGAGGCCATGATCAGAGGATTTGAGAAATACATGGAAGTAGATGGACAGGAAGGAGAAGAAGAACACATTCCTAATGTAACTGCTTGGCACAATGTTCCACATCCGGAGGAGAGATTAAGGTTTTGGTCTGATCCTGATACAGAACTAAAATTGGCCAAGGATACTGGTGTTACTGTTTTTCGTATGGGAATTGACTGGTCAAGAGTCATGCCTCATGAGCCAGTAAACAGCCTTAAAGAATCTGTCAACTATGCTGCATTGGAGCGCTATAAGTGGATTATCAATAGGGTTCGATCATATGGAATGAAGGTGATGCTCACTCTTTTTCATCACTCACTTCCACCCTGGGCCGGTGATTATGGAGGTTGGAAGCTGGAAAAGACAGTGGATTATTTCATGGATTTTACCAGGCTTGTTGTTGACAGTGTATCAGATTTAGTAGACTATTGGGTAACATTTAACGAGCCCCATGTCTTCTGTATGCTTACTTACTGTTCTGGAACCTGGCCTGGTGGACATCCTGATATGCTAGAGGCTGCTACTTCCGCACTTCCTACAGGTGTTTTCCAGCAGGCCATGCATTGGATATCTGTTGCACACTCAAAGGCATATGATTACATCCACGAACTCAGTAACTCATCAAATCAAATAGTTGGTGTCGCACATCATGTGTCGTTTATGCGACCCTATGGTCTGTTTGATATTGCTGCCGTTTCACTGGCTAATTCATTGAGTCTCTTCCCATTCATCGATGAAATATCCAAAAAGATGGACTTTATAGGCGTTAACTATTACGGCCAGGAAGCGATTTCGGGTGCTGGCCTGAAGCTTGTGGAAAATGATGAGTACAGTGAATCTGGACGTGGAGTATATCCTGATGGCTTATATCGCATGTTACTTCAGTTCCATGAAAGATACAAGCATCTAAATGTCCCTTTCATTATTGCTGAAAATGGAATTGCAGATGAGACTGATTTGATCCGCCGACCATATCTCTTGGAGCATTTACTTGCTGTTTATGCAGCTATGATCGAGGGTGTGCCTGTGCTTGGTTACCTCTTCTGGACTATTTCTGATAACTGGGAGTGGGCTGATGGATATGGTCCCAAGTTTGGACTTGTCGCAGTTGACCGAGCAAATAATCTTGCACGGATTCCTCGCCCTTCTTACCATCTGTTTTCTAAAGTTGTAAGTACCGGTAAAGTTACATGCGATGATCGTGATAACGCTTGGAATGAACTTCAAAGAGCCGCTAAAGAGAAGAAGACAAGACCATTTTATCGGGCTGTGGACAAAAATGGTTTAATGTTAGCAGGTGGACTTGATGAACCCATACAGCGGCCTTATATAGAAAGAGATTGGCGGTTTGGTCATTATGAGATGGATGACCTCCAGGATTACTTAAGCCAATTCTCAAGATTCATTATTCAACGATTCTCCCTAAAAAGGAAAAAGAAATCTCAAAAGGAGCAATTAGTTCTGCACCCGCTTTGA

Protein sequence

>Msa1332690

MTIVSLFLTATKLAGALVTLTVAANAFSFSRFRNKNLRRFTSPIDESSDTLADFNITEEENGFFFGLATAPAHVEDRLDDAWIQFAEQESVAGAEQKVDALMGSATADGGSQQAISSAQRGLKGNKKSLKVAMEAMIRGFEKYMEVDGQEGEEEHIPNVTAWHNVPHPEERLRFWSDPDTELKLAKDTGVTVFRMGIDWSRVMPHEPVNSLKESVNYAALERYKWIINRVRSYGMKVMLTLFHHSLPPWAGDYGGWKLEKTVDYFMDFTRLVVDSVSDLVDYWVTFNEPHVFCMLTYCSGTWPGGHPDMLEAATSALPTGVFQQAMHWISVAHSKAYDYIHELSNSSNQIVGVAHHVSFMRPYGLFDIAAVSLANSLSLFPFIDEISKKMDFIGVNYYGQEAISGAGLKLVENDEYSESGRGVYPDGLYRMLLQFHERYKHLNVPFIIAENGIADETDLIRRPYLLEHLLAVYAAMIEGVPVLGYLFWTISDNWEWADGYGPKFGLVAVDRANNLARIPRPSYHLFSKVVSTGKVTCDDRDNAWNELQRAAKEKKTRPFYRAVDKNGLMLAGGLDEPIQRPYIERDWRFGHYEMDDLQDYLSQFSRFIIQRFSLKRKKKSQKEQLVLHPL*