AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1363070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363070 MtrunA17_Chr4g0070211 75.000 72 15 1 8 79 3 71 2.03e-30 103
Msa1363070 MtrunA17_Chr2g0321051 36.893 103 60 2 9 111 8 105 1.39e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363070 AT3G17210.1 62.500 112 39 1 1 112 1 109 2.60e-45 142
Msa1363070 AT3G17210.2 62.500 112 39 1 1 112 1 109 2.60e-45 142
Msa1363070 AT5G22580.1 34.021 97 59 2 9 105 7 98 8.53e-15 65.5

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTCCTCAATCTTCTCT+TGG 0.329948 tig0018025:-113359 None:intergenic
CAAAGTGAAAGAGGAGGAAA+TGG 0.338863 tig0018025:+113278 Msa1363070:exon
GATTCAGGTAACAAATAATA+AGG 0.348275 tig0018025:-113677 None:intergenic
AATGAGTAAATCCTTGATTC+AGG 0.352373 tig0018025:-113692 None:intergenic
AGGAGGGAGCAAAGTGAAAG+AGG 0.406040 tig0018025:+113269 Msa1363070:five_prime_UTR
TTACCAATGGAATGACTTCA+TGG 0.420824 tig0018025:-113418 None:intergenic
ATTATTTGTTACCTGAATCA+AGG 0.432767 tig0018025:+113681 Msa1363070:CDS
AAAGTTGCTCGTTCCTTGCT+TGG 0.439365 tig0018025:+113788 Msa1363070:CDS
GAGTTCCTCAATCTTCTCTT+GGG 0.443255 tig0018025:-113358 None:intergenic
GTAGTGAAGAACATAGTGAT+TGG 0.457715 tig0018025:+113315 Msa1363070:CDS
AGTGAAAGAGGAGGAAATGG+AGG 0.502250 tig0018025:+113281 Msa1363070:exon
GAGGAAATGGAGGAAGCAAA+AGG 0.502851 tig0018025:+113291 Msa1363070:exon
TGGGATGAGATTGACGAGAT+TGG 0.516286 tig0018025:-113394 None:intergenic
ATTCAAAGGTTAACGATGGT+GGG 0.545431 tig0018025:-113837 None:intergenic
TCCTTGCTTGGAGAAAGTGC+TGG 0.548785 tig0018025:+113800 Msa1363070:CDS
CACCCATGAAGTCATTCCAT+TGG 0.553232 tig0018025:+113415 Msa1363070:CDS
TGAATCAACATTTGAGAGTG+TGG 0.555452 tig0018025:+113719 Msa1363070:CDS
TTTGCATACTCAACATGAGC+AGG 0.558557 tig0018025:-113768 None:intergenic
GAAGATTGAGGAACTCATCA+AGG 0.571882 tig0018025:+113365 Msa1363070:CDS
GCCAGCACTTTCTCCAAGCA+AGG 0.576667 tig0018025:-113801 None:intergenic
GATTCAAAGGTTAACGATGG+TGG 0.578082 tig0018025:-113838 None:intergenic
GAGATTGACGAGATTGGCAA+AGG 0.580515 tig0018025:-113388 None:intergenic
AGGGAGCAAAGTGAAAGAGG+AGG 0.582719 tig0018025:+113272 Msa1363070:five_prime_UTR
CAATGGAATGACTTCATGGG+TGG 0.583790 tig0018025:-113414 None:intergenic
AATCTCCCAAGAGAAGATTG+AGG 0.597731 tig0018025:+113353 Msa1363070:CDS
ATGAGCTACATACTCTGCAA+TGG 0.614733 tig0018025:-113745 None:intergenic
TACCAATGGAATGACTTCAT+GGG 0.631729 tig0018025:-113417 None:intergenic
AAGAAACACTGCAATGCACG+AGG 0.694375 tig0018025:+113218 None:intergenic
AATGGAATGACTTCATGGGT+GGG 0.706119 tig0018025:-113413 None:intergenic
ACGGATTCAAAGGTTAACGA+TGG 0.711712 tig0018025:-113841 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAAGTTTTTTAAGTTT+TGG - tig0018025:113520-113539 None:intergenic 10.0%
!!! TATACATGTCTTATTTTTTT+AGG + tig0018025:113637-113656 Msa1363070:intron 15.0%
!!! TTGTAGTATACAGCTATTTT+AGG + tig0018025:113249-113268 Msa1363070:five_prime_UTR 25.0%
!! TTTAAGTTTCATCTTACCAA+TGG - tig0018025:113434-113453 None:intergenic 25.0%
! GATTCAGGTAACAAATAATA+AGG - tig0018025:113680-113699 None:intergenic 25.0%
!! ATTATTTGTTACCTGAATCA+AGG + tig0018025:113681-113700 Msa1363070:CDS 25.0%
!! TAGTATACAGCTATTTTAGG+AGG + tig0018025:113252-113271 Msa1363070:five_prime_UTR 30.0%
!! AGTATACAGCTATTTTAGGA+GGG + tig0018025:113253-113272 Msa1363070:five_prime_UTR 30.0%
AATGAGTAAATCCTTGATTC+AGG - tig0018025:113695-113714 None:intergenic 30.0%
GTAGTGAAGAACATAGTGAT+TGG + tig0018025:113315-113334 Msa1363070:CDS 35.0%
TACCAATGGAATGACTTCAT+GGG - tig0018025:113420-113439 None:intergenic 35.0%
TTACCAATGGAATGACTTCA+TGG - tig0018025:113421-113440 None:intergenic 35.0%
!! ATAGTCTAGACTGCTTTTGT+CGG - tig0018025:113470-113489 None:intergenic 35.0%
TGAATCAACATTTGAGAGTG+TGG + tig0018025:113719-113738 Msa1363070:CDS 35.0%
CAAAGTGAAAGAGGAGGAAA+TGG + tig0018025:113278-113297 Msa1363070:exon 40.0%
AATCTCCCAAGAGAAGATTG+AGG + tig0018025:113353-113372 Msa1363070:CDS 40.0%
GAGTTCCTCAATCTTCTCTT+GGG - tig0018025:113361-113380 None:intergenic 40.0%
TGAGTTCCTCAATCTTCTCT+TGG - tig0018025:113362-113381 None:intergenic 40.0%
GAAGATTGAGGAACTCATCA+AGG + tig0018025:113365-113384 Msa1363070:CDS 40.0%
AATGGAATGACTTCATGGGT+GGG - tig0018025:113416-113435 None:intergenic 40.0%
ATGAGCTACATACTCTGCAA+TGG - tig0018025:113748-113767 None:intergenic 40.0%
TTTGCATACTCAACATGAGC+AGG - tig0018025:113771-113790 None:intergenic 40.0%
AGTGAAAGAGGAGGAAATGG+AGG + tig0018025:113281-113300 Msa1363070:exon 45.0%
GAGGAAATGGAGGAAGCAAA+AGG + tig0018025:113291-113310 Msa1363070:exon 45.0%
GAGATTGACGAGATTGGCAA+AGG - tig0018025:113391-113410 None:intergenic 45.0%
TGGGATGAGATTGACGAGAT+TGG - tig0018025:113397-113416 None:intergenic 45.0%
CAATGGAATGACTTCATGGG+TGG - tig0018025:113417-113436 None:intergenic 45.0%
CACCCATGAAGTCATTCCAT+TGG + tig0018025:113415-113434 Msa1363070:CDS 45.0%
! GATTTTCGCTAAGTGGACGA+GGG - tig0018025:113556-113575 None:intergenic 45.0%
! TACCAGCGATTTTCGCTAAG+TGG - tig0018025:113563-113582 None:intergenic 45.0%
AAAGTTGCTCGTTCCTTGCT+TGG + tig0018025:113788-113807 Msa1363070:CDS 45.0%
AGGAGGGAGCAAAGTGAAAG+AGG + tig0018025:113269-113288 Msa1363070:five_prime_UTR 50.0%
AGGGAGCAAAGTGAAAGAGG+AGG + tig0018025:113272-113291 Msa1363070:five_prime_UTR 50.0%
! CGATTTTCGCTAAGTGGACG+AGG - tig0018025:113557-113576 None:intergenic 50.0%
GTCCACTTAGCGAAAATCGC+TGG + tig0018025:113558-113577 Msa1363070:intron 50.0%
! TCCTTGCTTGGAGAAAGTGC+TGG + tig0018025:113800-113819 Msa1363070:CDS 50.0%
GCCAGCACTTTCTCCAAGCA+AGG - tig0018025:113804-113823 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0018025 gene 113226 113857 113226 ID=Msa1363070;Name=Msa1363070
tig0018025 mRNA 113226 113857 113226 ID=Msa1363070-mRNA-1;Parent=Msa1363070;Name=Msa1363070-mRNA-1;_AED=0.36;_eAED=0.37;_QI=71|0|0.5|1|1|1|2|0|112
tig0018025 exon 113226 113436 113226 ID=Msa1363070-mRNA-1:exon:13406;Parent=Msa1363070-mRNA-1
tig0018025 exon 113659 113857 113659 ID=Msa1363070-mRNA-1:exon:13407;Parent=Msa1363070-mRNA-1
tig0018025 five_prime_UTR 113226 113296 113226 ID=Msa1363070-mRNA-1:five_prime_utr;Parent=Msa1363070-mRNA-1
tig0018025 CDS 113297 113436 113297 ID=Msa1363070-mRNA-1:cds;Parent=Msa1363070-mRNA-1
tig0018025 CDS 113659 113857 113659 ID=Msa1363070-mRNA-1:cds;Parent=Msa1363070-mRNA-1
Gene Sequence

>Msa1363070

ATGGAGGAAGCAAAAGGAGTAGTGAAGAACATAGTGATTGGAAAGTTCAAAGATGAAATCTCCCAAGAGAAGATTGAGGAACTCATCAAGGCCTTTGCCAATCTCGTCAATCTCATCCCACCCATGAAGTCATTCCATTGGAATTGTTGTTATACTTTCCTTATTATTTGTTACCTGAATCAAGGATTTACTCATTTCTTTGAATCAACATTTGAGAGTGTGGAAGCCATTGCAGAGTATGTAGCTCATCCTGCTCATGTTGAGTATGCAAAGTTGCTCGTTCCTTGCTTGGAGAAAGTGCTGGCATTTGATTATAAGCCCACCATCGTTAACCTTTGA

Protein sequence

>Msa1363070

MEEAKGVVKNIVIGKFKDEISQEKIEELIKAFANLVNLIPPMKSFHWNCCYTFLIICYLNQGFTHFFESTFESVEAIAEYVAHPAHVEYAKLLVPCLEKVLAFDYKPTIVNL*