Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045937.t1 | XP_013445680.1 | 88.3 | 412 | 13 | 2 | 1 | 377 | 1 | 412 | 1.80E-207 | 731.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045937.t1 | Q64LD2 | 34.9 | 375 | 219 | 5 | 18 | 378 | 178 | 541 | 1.6e-66 | 255.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045937.t1 | A0A072TR58 | 88.3 | 412 | 13 | 2 | 1 | 377 | 1 | 412 | 1.3e-207 | 731.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045937.t1 | MTR_8g467040 | 88.350 | 412 | 13 | 2 | 1 | 377 | 1 | 412 | 0.0 | 736 |
| MS.gene045937.t1 | MTR_4g102520 | 33.119 | 311 | 194 | 4 | 72 | 378 | 282 | 582 | 4.91e-52 | 184 |
| MS.gene045937.t1 | MTR_1g072740 | 32.881 | 295 | 176 | 6 | 72 | 355 | 281 | 564 | 7.22e-41 | 153 |
| MS.gene045937.t1 | MTR_1g072140 | 26.282 | 312 | 201 | 11 | 73 | 376 | 16 | 306 | 9.43e-24 | 101 |
| MS.gene045937.t1 | MTR_7g086360 | 26.974 | 304 | 202 | 9 | 80 | 377 | 51 | 340 | 1.51e-23 | 101 |
| MS.gene045937.t1 | MTR_4g119620 | 26.282 | 312 | 204 | 10 | 70 | 370 | 13 | 309 | 9.45e-21 | 93.2 |
| MS.gene045937.t1 | MTR_3g449990 | 24.606 | 317 | 218 | 11 | 66 | 377 | 252 | 552 | 1.85e-13 | 72.8 |
| MS.gene045937.t1 | MTR_8g092620 | 22.591 | 301 | 187 | 10 | 119 | 382 | 8 | 299 | 2.31e-13 | 70.9 |
| MS.gene045937.t1 | MTR_8g092620 | 22.559 | 297 | 184 | 10 | 119 | 378 | 15 | 302 | 3.64e-13 | 70.9 |
| MS.gene045937.t1 | MTR_1g069345 | 23.235 | 340 | 231 | 11 | 54 | 384 | 189 | 507 | 5.28e-12 | 68.2 |
| MS.gene045937.t1 | MTR_1g100687 | 43.548 | 62 | 35 | 0 | 376 | 437 | 164 | 225 | 9.01e-12 | 65.1 |
| MS.gene045937.t1 | MTR_3g045130 | 25.751 | 233 | 145 | 7 | 65 | 288 | 294 | 507 | 2.88e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045937.t1 | AT5G54520 | 65.318 | 346 | 117 | 2 | 32 | 377 | 111 | 453 | 2.50e-176 | 501 |
| MS.gene045937.t1 | AT1G10580 | 34.062 | 320 | 197 | 4 | 63 | 378 | 261 | 570 | 8.82e-56 | 194 |
| MS.gene045937.t1 | AT4G02730 | 26.384 | 307 | 197 | 10 | 73 | 370 | 32 | 318 | 1.64e-25 | 107 |
| MS.gene045937.t1 | AT2G43770 | 25.364 | 343 | 237 | 9 | 39 | 377 | 11 | 338 | 1.43e-22 | 98.6 |
| MS.gene045937.t1 | AT3G49660 | 26.198 | 313 | 201 | 10 | 77 | 378 | 17 | 310 | 1.61e-21 | 95.1 |
| MS.gene045937.t1 | AT5G13480 | 29.534 | 193 | 118 | 7 | 78 | 262 | 231 | 413 | 2.67e-12 | 69.3 |
| MS.gene045937.t1 | AT5G13480 | 29.534 | 193 | 118 | 7 | 78 | 262 | 231 | 413 | 3.15e-12 | 68.9 |
| MS.gene045937.t1 | AT5G13480 | 29.534 | 193 | 118 | 7 | 78 | 262 | 254 | 436 | 3.36e-12 | 68.9 |
| MS.gene045937.t1 | AT5G13480 | 29.534 | 193 | 118 | 7 | 78 | 262 | 243 | 425 | 3.42e-12 | 68.9 |
| MS.gene045937.t1 | AT5G64730 | 23.473 | 311 | 219 | 9 | 72 | 381 | 6 | 298 | 5.38e-12 | 67.0 |
| MS.gene045937.t1 | AT5G43920 | 21.622 | 333 | 239 | 9 | 56 | 382 | 199 | 515 | 3.28e-11 | 65.9 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGAAATTGCACTTAAT+TGG | 0.089098 | 8.3:-43921870 | None:intergenic |
| TATGAGAACCATGGGGTTTC+TGG | 0.132809 | 8.3:+43921843 | MS.gene045937:CDS |
| CATAACTATAATCGAAATTT+AGG | 0.181559 | 8.3:+43915550 | MS.gene045937:CDS |
| AATTCAAGTCTCTTTCTTTC+TGG | 0.210960 | 8.3:+43915475 | MS.gene045937:CDS |
| GTCCATGCATGCTTGGTTAA+AGG | 0.247778 | 8.3:-43921982 | None:intergenic |
| GAGAGAAGGAGAAGGGAGAT+AGG | 0.255834 | 8.3:-43912463 | None:intergenic |
| GCAATAACATTAGGCAAAAC+TGG | 0.260777 | 8.3:-43922017 | None:intergenic |
| AAGTGCAATTTCAGCTTAGA+TGG | 0.263435 | 8.3:+43921876 | MS.gene045937:CDS |
| GCTCACCTCCTTGCATCTGC+TGG | 0.268487 | 8.3:+43915220 | MS.gene045937:intron |
| CTGAGATGATTTCAGTCGTT+TGG | 0.277602 | 8.3:-43912418 | None:intergenic |
| TCAACAATGCTACAATGTTA+TGG | 0.295169 | 8.3:-43914809 | None:intergenic |
| TTCTTCTTCGTCTGAGGAAT+TGG | 0.307219 | 8.3:-43912391 | None:intergenic |
| CAACAATGCTACAATGTTAT+GGG | 0.316478 | 8.3:-43914808 | None:intergenic |
| TCTCCTTCTCTCGTTCTTCC+AGG | 0.321145 | 8.3:+43912474 | MS.gene045937:CDS |
| ATGTCTTCAAGTGATGTATC+TGG | 0.329819 | 8.3:+43915616 | MS.gene045937:CDS |
| GTGAGAATTCTATTATCGTT+TGG | 0.344806 | 8.3:+43915650 | MS.gene045937:CDS |
| AGCTGAAATTGCACTTAATT+GGG | 0.350793 | 8.3:-43921869 | None:intergenic |
| TGAGAATTCTATTATCGTTT+GGG | 0.353439 | 8.3:+43915651 | MS.gene045937:CDS |
| ATTCAAGTCTCTTTCTTTCT+GGG | 0.354972 | 8.3:+43915476 | MS.gene045937:CDS |
| GCTTGTCAGGTACCGAGTTC+AGG | 0.356109 | 8.3:-43914857 | None:intergenic |
| TTGTATTTGTTGAGTCTGTA+TGG | 0.357549 | 8.3:-43921816 | None:intergenic |
| GATGTCGAGTTTACAACAAA+TGG | 0.364650 | 8.3:+43915583 | MS.gene045937:CDS |
| TTCAAAGAAGATCAAATTGT+TGG | 0.374362 | 8.3:+43915430 | MS.gene045937:CDS |
| AGAAATTAAAGCCAACAATA+TGG | 0.377390 | 8.3:+43922769 | MS.gene045937:CDS |
| TGAGATGATTTCAGTCGTTT+GGG | 0.386304 | 8.3:-43912417 | None:intergenic |
| GGAGAAGGGAGATAGGGTTT+TGG | 0.400705 | 8.3:-43912456 | None:intergenic |
| AAGCTATCTGCAACTCTGTC+TGG | 0.412532 | 8.3:+43914876 | MS.gene045937:CDS |
| ATGTTATTGCTTCATGCTCA+TGG | 0.419798 | 8.3:+43922030 | MS.gene045937:CDS |
| AGAAAGAGACTTGAATTGTC+TGG | 0.420157 | 8.3:-43915469 | None:intergenic |
| CCTCCTTGCATCTGCTGGGA+TGG | 0.421099 | 8.3:+43915225 | MS.gene045937:CDS |
| ATGAGAACCATGGGGTTTCT+GGG | 0.423886 | 8.3:+43921844 | MS.gene045937:CDS |
| GAGCATGAAGCAATAACATT+AGG | 0.424741 | 8.3:-43922026 | None:intergenic |
| TGATATCCCATAGCTTGATA+TGG | 0.425356 | 8.3:-43915510 | None:intergenic |
| ACAGGTACCATTGTCTAATC+AGG | 0.425682 | 8.3:+43915684 | MS.gene045937:CDS |
| AGGTTGGTTGATGTTGAAAA+GGG | 0.433049 | 8.3:+43915391 | MS.gene045937:CDS |
| ATATCCAACTTATCACCAAT+TGG | 0.438057 | 8.3:-43941984 | None:intergenic |
| AAGGTTGGTTGATGTTGAAA+AGG | 0.438941 | 8.3:+43915390 | MS.gene045937:CDS |
| CTCACCTCCTTGCATCTGCT+GGG | 0.448124 | 8.3:+43915221 | MS.gene045937:CDS |
| AATGCCTCATACCATATTGT+TGG | 0.448679 | 8.3:-43922780 | None:intergenic |
| AGAGTTGCAGATAGCTTGTC+AGG | 0.454221 | 8.3:-43914870 | None:intergenic |
| ATTTGTTGAGTCTGTATGGT+GGG | 0.464723 | 8.3:-43921812 | None:intergenic |
| GATGGGAAGAAGCTTGCATC+TGG | 0.466105 | 8.3:+43921894 | MS.gene045937:CDS |
| AGAGAAGGAGAAGGGAGATA+GGG | 0.471173 | 8.3:-43912462 | None:intergenic |
| AAGCTATGGGATATCAGAAC+TGG | 0.476171 | 8.3:+43915517 | MS.gene045937:CDS |
| GCTTCTGAATCAGGTTTATG+TGG | 0.478796 | 8.3:+43921695 | MS.gene045937:intron |
| GGGCACTTCTTGCTTTCTTG+TGG | 0.484923 | 8.3:+43915352 | MS.gene045937:CDS |
| CTTGCATCTGGCTCTTCAGA+TGG | 0.486666 | 8.3:+43921906 | MS.gene045937:CDS |
| TTCAAGTCTCTTTCTTTCTG+GGG | 0.490030 | 8.3:+43915477 | MS.gene045937:CDS |
| TCAAGTCTCTTTCTTTCTGG+GGG | 0.500922 | 8.3:+43915478 | MS.gene045937:CDS |
| CAAAGGGCCATATCAAGCTA+TGG | 0.503725 | 8.3:+43915503 | MS.gene045937:CDS |
| ATGTCGAGTTTACAACAAAT+GGG | 0.515879 | 8.3:+43915584 | MS.gene045937:CDS |
| GGCCTTTAACCAAGCATGCA+TGG | 0.516184 | 8.3:+43921980 | MS.gene045937:CDS |
| GGTTATGGTGCTCAACCAAT+TGG | 0.523247 | 8.3:+43941969 | MS.gene045937:CDS |
| ACAAGAGATATGAGAACCAT+GGG | 0.529817 | 8.3:+43921835 | MS.gene045937:CDS |
| CATCTTTCACTGCTGCATTG+TGG | 0.536720 | 8.3:-43915312 | None:intergenic |
| CATAAATATAGGTAGTATCT+CGG | 0.537273 | 8.3:+43914773 | MS.gene045937:intron |
| TGTTATTGCTTCATGCTCAT+GGG | 0.537623 | 8.3:+43922031 | MS.gene045937:CDS |
| CGTTTGGGATGTGTCAAGAC+AGG | 0.538329 | 8.3:+43915666 | MS.gene045937:CDS |
| CAGCAGTGAAAGATGTGAAA+TGG | 0.541653 | 8.3:+43915320 | MS.gene045937:CDS |
| TCAACCAATTGGTGATAAGT+TGG | 0.544317 | 8.3:+43941980 | MS.gene045937:CDS |
| TAAGAAACTTGATGTCATGC+GGG | 0.545037 | 8.3:+43942079 | MS.gene045937:CDS |
| TGTCTTCAAGTGATGTATCT+GGG | 0.549234 | 8.3:+43915617 | MS.gene045937:CDS |
| ATTGTTGAAAAGTAAAGCAA+AGG | 0.551190 | 8.3:+43914824 | MS.gene045937:CDS |
| GGAAGAACGAGAGAAGGAGA+AGG | 0.555955 | 8.3:-43912471 | None:intergenic |
| ATTAGGCAAAACTGGGTGGA+AGG | 0.557065 | 8.3:-43922009 | None:intergenic |
| AGTGCAATTTCAGCTTAGAT+GGG | 0.561560 | 8.3:+43921877 | MS.gene045937:CDS |
| TTGTTGAAAAGTAAAGCAAA+GGG | 0.562663 | 8.3:+43914825 | MS.gene045937:CDS |
| AAAGGGCCATATCAAGCTAT+GGG | 0.566987 | 8.3:+43915504 | MS.gene045937:CDS |
| GGTTGATGTTGAAAAGGGGA+TGG | 0.571477 | 8.3:+43915396 | MS.gene045937:CDS |
| GAAGAACGAGAGAAGGAGAA+GGG | 0.579478 | 8.3:-43912470 | None:intergenic |
| AGAGACTTGAATTGTCTGGA+TGG | 0.583021 | 8.3:-43915465 | None:intergenic |
| TTTGTTGAGTCTGTATGGTG+GGG | 0.587259 | 8.3:-43921811 | None:intergenic |
| ATACATTGGTCATCAACTCA+TGG | 0.592308 | 8.3:+43914921 | MS.gene045937:CDS |
| GTTGAGTCTGTATGGTGGGG+TGG | 0.595657 | 8.3:-43921808 | None:intergenic |
| GTGGATATGATTGCACATCA+AGG | 0.600456 | 8.3:+43915371 | MS.gene045937:CDS |
| GACAATCATCAATGTCTCGT+CGG | 0.602425 | 8.3:-43942055 | None:intergenic |
| CAATAACATTAGGCAAAACT+GGG | 0.602442 | 8.3:-43922016 | None:intergenic |
| TGTCCATCCCAGCAGATGCA+AGG | 0.605709 | 8.3:-43915228 | None:intergenic |
| GTGCAATGGATCTCTTGAGT+TGG | 0.606378 | 8.3:+43942009 | MS.gene045937:CDS |
| AGGCAATGTCCATGCATGCT+TGG | 0.606698 | 8.3:-43921989 | None:intergenic |
| AACAATGCTACAATGTTATG+GGG | 0.613093 | 8.3:-43914807 | None:intergenic |
| GAGCTGTCAATGCTATACAT+TGG | 0.614108 | 8.3:+43914907 | MS.gene045937:CDS |
| TATTTGTTGAGTCTGTATGG+TGG | 0.615770 | 8.3:-43921813 | None:intergenic |
| CTACCTGGAAGAACGAGAGA+AGG | 0.618272 | 8.3:-43912477 | None:intergenic |
| ATTGCTTCATGCTCATGGGA+TGG | 0.618591 | 8.3:+43922035 | MS.gene045937:CDS |
| TACAAGAGATATGAGAACCA+TGG | 0.619211 | 8.3:+43921834 | MS.gene045937:CDS |
| AAGGGGTGAATTCTAATGCA+AGG | 0.619618 | 8.3:-43921732 | None:intergenic |
| GTAAGAAACTTGATGTCATG+CGG | 0.625323 | 8.3:+43942078 | MS.gene045937:CDS |
| TAAGTTGGATATGTGTGCAA+TGG | 0.626693 | 8.3:+43941995 | MS.gene045937:CDS |
| ATATGATTGCACATCAAGGT+TGG | 0.628197 | 8.3:+43915375 | MS.gene045937:CDS |
| TTGGGAACCCAGAAACCCCA+TGG | 0.636691 | 8.3:-43921851 | None:intergenic |
| ATCTGCTGGGATGGACAATG+TGG | 0.644625 | 8.3:+43915234 | MS.gene045937:CDS |
| GATGTGAAATGGTCTCAACA+AGG | 0.649349 | 8.3:+43915331 | MS.gene045937:CDS |
| TAACATTAGGCAAAACTGGG+TGG | 0.660319 | 8.3:-43922013 | None:intergenic |
| ATGTGAAATGGTCTCAACAA+GGG | 0.661548 | 8.3:+43915332 | MS.gene045937:CDS |
| GGTTGGTTGATGTTGAAAAG+GGG | 0.664123 | 8.3:+43915392 | MS.gene045937:CDS |
| GGGTCATCAAAACCTGAACT+CGG | 0.669945 | 8.3:+43914845 | MS.gene045937:CDS |
| TATCTCACCTGATTAGACAA+TGG | 0.678265 | 8.3:-43915691 | None:intergenic |
| AAGAAACTTGATGTCATGCG+GGG | 0.690083 | 8.3:+43942080 | MS.gene045937:CDS |
| CCATCCCAGCAGATGCAAGG+AGG | 0.698307 | 8.3:-43915225 | None:intergenic |
| AAATATAGGTAGTATCTCGG+AGG | 0.721969 | 8.3:+43914776 | MS.gene045937:intron |
| CAAGAGATATGAGAACCATG+GGG | 0.792722 | 8.3:+43921836 | MS.gene045937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 43912366 | 43948615 | 43912366 | ID=MS.gene045937 |
| chr8.3 | mRNA | 43912366 | 43948615 | 43912366 | ID=MS.gene045937.t1;Parent=MS.gene045937 |
| chr8.3 | exon | 43912366 | 43912495 | 43912366 | ID=MS.gene045937.t1.exon1;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43912366 | 43912495 | 43912366 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43914784 | 43914942 | 43914784 | ID=MS.gene045937.t1.exon2;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43914784 | 43914942 | 43914784 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43915221 | 43915705 | 43915221 | ID=MS.gene045937.t1.exon3;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43915221 | 43915705 | 43915221 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43921708 | 43922059 | 43921708 | ID=MS.gene045937.t1.exon4;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43921708 | 43922059 | 43921708 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43922763 | 43922790 | 43922763 | ID=MS.gene045937.t1.exon5;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43922763 | 43922790 | 43922763 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43941944 | 43942106 | 43941944 | ID=MS.gene045937.t1.exon6;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43941944 | 43942106 | 43941944 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| chr8.3 | exon | 43948598 | 43948615 | 43948598 | ID=MS.gene045937.t1.exon7;Parent=MS.gene045937.t1 |
| chr8.3 | CDS | 43948598 | 43948615 | 43948598 | ID=cds.MS.gene045937.t1;Parent=MS.gene045937.t1 |
| Gene Sequence |
| Protein sequence |
