AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25515

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25515.t1 MTR_8g467040 96.746 338 11 0 87 424 79 416 0.0 685
MS.gene25515.t1 MTR_4g102520 33.226 310 193 4 115 420 282 581 5.73e-51 181
MS.gene25515.t1 MTR_1g072740 32.881 295 176 6 115 398 281 564 7.17e-40 150
MS.gene25515.t1 MTR_7g086360 26.409 337 225 11 94 424 25 344 7.37e-24 102
MS.gene25515.t1 MTR_1g072140 26.367 311 200 11 116 418 16 305 3.86e-23 99.4
MS.gene25515.t1 MTR_4g119620 26.282 312 204 10 113 413 13 309 1.84e-20 92.0
MS.gene25515.t1 MTR_3g449990 24.375 320 221 11 109 423 252 555 7.69e-14 73.9
MS.gene25515.t1 MTR_8g092620 22.667 300 186 10 162 424 8 298 2.31e-13 70.9
MS.gene25515.t1 MTR_8g092620 22.635 296 183 10 162 420 15 301 1.20e-12 68.9
MS.gene25515.t1 MTR_1g069345 23.214 336 228 11 97 423 189 503 1.54e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25515.t1 AT5G54520 66.272 338 114 0 87 424 120 457 5.38e-178 504
MS.gene25515.t1 AT1G10580 33.746 323 200 4 106 424 261 573 4.39e-55 192
MS.gene25515.t1 AT4G02730 26.384 307 197 10 116 413 32 318 9.05e-25 104
MS.gene25515.t1 AT2G43770 26.144 306 210 8 123 424 49 342 2.04e-22 98.2
MS.gene25515.t1 AT3G49660 26.032 315 203 10 120 423 17 312 8.22e-22 95.9
MS.gene25515.t1 AT5G13480 29.534 193 118 7 121 305 254 436 5.67e-12 68.2
MS.gene25515.t1 AT5G13480 29.534 193 118 7 121 305 231 413 6.77e-12 67.8
MS.gene25515.t1 AT5G13480 29.534 193 118 7 121 305 231 413 6.81e-12 67.8
MS.gene25515.t1 AT5G13480 29.534 193 118 7 121 305 243 425 7.14e-12 67.8
MS.gene25515.t1 AT5G64730 22.830 311 221 9 115 424 6 298 7.54e-12 66.2
MS.gene25515.t1 AT4G28450 22.263 274 186 9 121 382 60 318 2.22e-11 65.9
MS.gene25515.t1 AT5G43920 21.136 317 231 8 113 423 210 513 2.32e-11 65.9

Find 107 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTGAAATTGCACTTAAT+TGG 0.089098 8.2:-42334383 None:intergenic
TATGAGAACCATGGGGTTTC+TGG 0.132809 8.2:+42334356 MS.gene25515:CDS
AATTCAAGTCTCTTTCTTTC+TGG 0.210960 8.2:+42329164 MS.gene25515:CDS
CTCATGGGATGGAAGTATTT+TGG 0.214310 8.2:+42334559 MS.gene25515:CDS
GTCCATGCATGCTTGGTTAA+AGG 0.247778 8.2:-42334495 None:intergenic
GCAATAACATTAGGCAAAAC+TGG 0.260777 8.2:-42334530 None:intergenic
AAGTGCAATTTCAGCTTAGA+TGG 0.263435 8.2:+42334389 MS.gene25515:CDS
GCTCACCTCCTTGCATCTGC+TGG 0.268487 8.2:+42328909 MS.gene25515:intron
TCAACAATGCTACAATGTTA+TGG 0.295169 8.2:-42328498 None:intergenic
CATAACTATAATCGAAGTTT+AGG 0.313370 8.2:+42329239 MS.gene25515:CDS
CAACAATGCTACAATGTTAT+GGG 0.316478 8.2:-42328497 None:intergenic
ATGTCTTCAAGTGATGTATC+TGG 0.329819 8.2:+42329305 MS.gene25515:CDS
GTGAGAATTCTATTATCGTT+TGG 0.344806 8.2:+42329339 MS.gene25515:CDS
AGCTGAAATTGCACTTAATT+GGG 0.350793 8.2:-42334382 None:intergenic
TGAGAATTCTATTATCGTTT+GGG 0.353439 8.2:+42329340 MS.gene25515:CDS
ATTCAAGTCTCTTTCTTTCT+GGG 0.354972 8.2:+42329165 MS.gene25515:CDS
GCTTGTCAGGTACCGAGTTC+AGG 0.356109 8.2:-42328546 None:intergenic
GATGTCGAGTTTACAACAAA+TGG 0.364650 8.2:+42329272 MS.gene25515:CDS
TTATATTTGTTGAGTCTGTA+TGG 0.367208 8.2:-42334329 None:intergenic
TTCAAAGAAGATCAAATTGT+TGG 0.374362 8.2:+42329119 MS.gene25515:CDS
AAGCTATCTGCAACTCTGTC+TGG 0.412532 8.2:+42328565 MS.gene25515:CDS
ATGTTATTGCTTCATGCTCA+TGG 0.419798 8.2:+42334543 MS.gene25515:CDS
AGAAAGAGACTTGAATTGTC+TGG 0.420157 8.2:-42329158 None:intergenic
CCTCCTTGCATCTGCTGGGA+TGG 0.421099 8.2:+42328914 MS.gene25515:CDS
ATGAGAACCATGGGGTTTCT+GGG 0.423886 8.2:+42334357 MS.gene25515:CDS
GAGCATGAAGCAATAACATT+AGG 0.424741 8.2:-42334539 None:intergenic
TGATATCCCATAGCTTGATA+TGG 0.425356 8.2:-42329199 None:intergenic
ACAGGTACCATTGTCTAATC+AGG 0.425682 8.2:+42329373 MS.gene25515:CDS
TGGATGACTGATAATCATAA+AGG 0.429834 8.2:-42334451 None:intergenic
AGGTTGGTTGATGTTGAAAA+GGG 0.433049 8.2:+42329080 MS.gene25515:CDS
TAGAGATAGGTTCAACGATC+AGG 0.434580 8.2:+42327098 MS.gene25515:CDS
AAGGTTGGTTGATGTTGAAA+AGG 0.438941 8.2:+42329079 MS.gene25515:CDS
ACTGCACAGGAACACCGCCC+CGG 0.442783 8.2:-42327127 None:intergenic
ATCTGGTGTTCGTCGACTGT+GGG 0.447510 8.2:+42326917 MS.gene25515:CDS
CTCACCTCCTTGCATCTGCT+GGG 0.448124 8.2:+42328910 MS.gene25515:CDS
AGAGTTGCAGATAGCTTGTC+AGG 0.454221 8.2:-42328559 None:intergenic
TGAAATCCAGCGACGGATGC+GGG 0.456373 8.2:+42327072 MS.gene25515:CDS
GATGGGAAGAAGCTTGCATC+TGG 0.466105 8.2:+42334407 MS.gene25515:CDS
AAGCTATGGGATATCAGAAC+TGG 0.476171 8.2:+42329206 MS.gene25515:CDS
CTAACTCCCGCATCCGTCGC+TGG 0.476877 8.2:-42327078 None:intergenic
GCTTCTGAATCAGGTTTATG+TGG 0.478796 8.2:+42334208 MS.gene25515:intron
GGGCACTTCTTGCTTTCTTG+TGG 0.484923 8.2:+42329041 MS.gene25515:CDS
CTTGCATCTGGCTCTTCAGA+TGG 0.486666 8.2:+42334419 MS.gene25515:CDS
TTCAAGTCTCTTTCTTTCTG+GGG 0.490030 8.2:+42329166 MS.gene25515:CDS
AATCCAATACACCGCGGTTG+TGG 0.497945 8.2:-42327159 None:intergenic
TCAAGTCTCTTTCTTTCTGG+GGG 0.500922 8.2:+42329167 MS.gene25515:CDS
CAAAGGGCCATATCAAGCTA+TGG 0.503725 8.2:+42329192 MS.gene25515:CDS
CGTCTTTACTAATGACGATC+TGG 0.505308 8.2:+42326900 None:intergenic
CGGATGCGGGAGTTAGAGAT+AGG 0.506506 8.2:+42327085 MS.gene25515:CDS
ATGTCGAGTTTACAACAAAT+GGG 0.515879 8.2:+42329273 MS.gene25515:CDS
GGCCTTTAACCAAGCATGCA+TGG 0.516184 8.2:+42334493 MS.gene25515:CDS
CTGAAATCCAGCGACGGATG+CGG 0.530492 8.2:+42327071 MS.gene25515:CDS
ATAAGAGATATGAGAACCAT+GGG 0.533569 8.2:+42334348 MS.gene25515:CDS
CATCTTTCACTGCTGCATTG+TGG 0.536720 8.2:-42329001 None:intergenic
CATAAATATAGGTAGTATCT+CGG 0.537273 8.2:+42328462 MS.gene25515:intron
TGTTATTGCTTCATGCTCAT+GGG 0.537623 8.2:+42334544 MS.gene25515:CDS
CGTTTGGGATGTGTCAAGAC+AGG 0.538329 8.2:+42329355 MS.gene25515:CDS
CTGCACAGGAACACCGCCCC+GGG 0.540039 8.2:-42327126 None:intergenic
CAGCAGTGAAAGATGTGAAA+TGG 0.541653 8.2:+42329009 MS.gene25515:CDS
ATTTGTTGAGTCTGTATGGT+GGG 0.541991 8.2:-42334325 None:intergenic
GTTGAGTCTGTATGGTGGGT+AGG 0.542763 8.2:-42334321 None:intergenic
TCAACGATCAGGATCTGCCC+GGG 0.542837 8.2:+42327109 MS.gene25515:CDS
GCGGTGTTCCTGTGCAGTAG+AGG 0.548971 8.2:+42327132 MS.gene25515:CDS
TGTCTTCAAGTGATGTATCT+GGG 0.549234 8.2:+42329306 MS.gene25515:CDS
ATTGTTGAAAAGTAAAGCAA+AGG 0.551190 8.2:+42328513 MS.gene25515:CDS
ATTAGGCAAAACTGGGTGGA+AGG 0.557065 8.2:-42334522 None:intergenic
AGTGCAATTTCAGCTTAGAT+GGG 0.561560 8.2:+42334390 MS.gene25515:CDS
TTGTTGAAAAGTAAAGCAAA+GGG 0.562663 8.2:+42328514 MS.gene25515:CDS
CAATACACCGCGGTTGTGGA+AGG 0.562838 8.2:-42327155 None:intergenic
TTCAACGATCAGGATCTGCC+CGG 0.564348 8.2:+42327108 MS.gene25515:CDS
AAAGGGCCATATCAAGCTAT+GGG 0.566987 8.2:+42329193 MS.gene25515:CDS
GTGGAAGGCCTCTACTGCAC+AGG 0.569621 8.2:-42327140 None:intergenic
GGTTGATGTTGAAAAGGGGA+TGG 0.571477 8.2:+42329085 MS.gene25515:CDS
AGAGACTTGAATTGTCTGGA+TGG 0.583021 8.2:-42329154 None:intergenic
ATACATTGGTCATCAACTCA+TGG 0.592308 8.2:+42328610 MS.gene25515:CDS
TATAAGAGATATGAGAACCA+TGG 0.593911 8.2:+42334347 MS.gene25515:CDS
GTGGATATGATTGCACATCA+AGG 0.600456 8.2:+42329060 MS.gene25515:CDS
CAATAACATTAGGCAAAACT+GGG 0.602442 8.2:-42334529 None:intergenic
TGTCCATCCCAGCAGATGCA+AGG 0.605709 8.2:-42328917 None:intergenic
AGGCAATGTCCATGCATGCT+TGG 0.606698 8.2:-42334502 None:intergenic
TCACATACTATGTTGAGCAT+CGG 0.607175 8.2:-42326968 None:intergenic
AACAATGCTACAATGTTATG+GGG 0.613093 8.2:-42328496 None:intergenic
GATCTGGTGTTCGTCGACTG+TGG 0.613772 8.2:+42326916 MS.gene25515:CDS
GAGCTGTCAATGCTATACAT+TGG 0.614108 8.2:+42328596 MS.gene25515:CDS
ATTGCTTCATGCTCATGGGA+TGG 0.618591 8.2:+42334548 MS.gene25515:CDS
TATTTGTTGAGTCTGTATGG+TGG 0.619013 8.2:-42334326 None:intergenic
AAGGGGTGAATTCTAATGCA+AGG 0.619618 8.2:-42334245 None:intergenic
ATATGATTGCACATCAAGGT+TGG 0.628197 8.2:+42329064 MS.gene25515:CDS
AGAAAATGCGTGAAGTGGCG+TGG 0.636640 8.2:+42327011 MS.gene25515:CDS
TTGGGAACCCAGAAACCCCA+TGG 0.636691 8.2:-42334364 None:intergenic
ATCTGCTGGGATGGACAATG+TGG 0.644625 8.2:+42328923 MS.gene25515:CDS
GTGTGAGAAAATGCGTGAAG+TGG 0.644657 8.2:+42327006 MS.gene25515:CDS
CTGCTTCTGAAATCCAGCGA+CGG 0.645684 8.2:+42327065 MS.gene25515:CDS
GATGTGAAATGGTCTCAACA+AGG 0.649349 8.2:+42329020 MS.gene25515:CDS
TACTATGTTGAGCATCGGAG+TGG 0.658448 8.2:-42326963 None:intergenic
TAACATTAGGCAAAACTGGG+TGG 0.660319 8.2:-42334526 None:intergenic
ATGTGAAATGGTCTCAACAA+GGG 0.661548 8.2:+42329021 MS.gene25515:CDS
GGTTGGTTGATGTTGAAAAG+GGG 0.664123 8.2:+42329081 MS.gene25515:CDS
CAACGATCAGGATCTGCCCG+GGG 0.664425 8.2:+42327110 MS.gene25515:CDS
TCTGGTGTTCGTCGACTGTG+GGG 0.665190 8.2:+42326918 MS.gene25515:CDS
CGATCAGGATCTGCCCGGGG+CGG 0.665741 8.2:+42327113 MS.gene25515:CDS
GGGTCATCAAAACCTGAACT+CGG 0.669945 8.2:+42328534 MS.gene25515:CDS
TATCTCACCTGATTAGACAA+TGG 0.678265 8.2:-42329380 None:intergenic
GTAGAGGCCTTCCACAACCG+CGG 0.679407 8.2:+42327148 MS.gene25515:CDS
CCATCCCAGCAGATGCAAGG+AGG 0.698307 8.2:-42328914 None:intergenic
AAATATAGGTAGTATCTCGG+AGG 0.721969 8.2:+42328465 MS.gene25515:intron
TAAGAGATATGAGAACCATG+GGG 0.795950 8.2:+42334349 MS.gene25515:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.2 gene 42326911 42334592 42326911 ID=MS.gene25515
chr8.2 mRNA 42326911 42334592 42326911 ID=MS.gene25515.t1;Parent=MS.gene25515
chr8.2 exon 42326911 42327169 42326911 ID=MS.gene25515.t1.exon1;Parent=MS.gene25515.t1
chr8.2 CDS 42326911 42327169 42326911 ID=cds.MS.gene25515.t1;Parent=MS.gene25515.t1
chr8.2 exon 42328473 42328631 42328473 ID=MS.gene25515.t1.exon2;Parent=MS.gene25515.t1
chr8.2 CDS 42328473 42328631 42328473 ID=cds.MS.gene25515.t1;Parent=MS.gene25515.t1
chr8.2 exon 42328910 42329394 42328910 ID=MS.gene25515.t1.exon3;Parent=MS.gene25515.t1
chr8.2 CDS 42328910 42329394 42328910 ID=cds.MS.gene25515.t1;Parent=MS.gene25515.t1
chr8.2 exon 42334221 42334592 42334221 ID=MS.gene25515.t1.exon4;Parent=MS.gene25515.t1
chr8.2 CDS 42334221 42334592 42334221 ID=cds.MS.gene25515.t1;Parent=MS.gene25515.t1
Gene Sequence

>MS.gene25515

ATGACGATCTGGTGTTCGTCGACTGTGGGGAAGAAACCTGCAAAACAGCTAACCACTCCGATGCTCAACATAGTATGTGAACTTGAGAGAGAGAAGTGTGAGAAAATGCGTGAAGTGGCGTGGAGCTATGTTTATATAGCTGAGCTCAGCGTAACTGCTTCTGAAATCCAGCGACGGATGCGGGAGTTAGAGATAGGTTCAACGATCAGGATCTGCCCGGGGCGGTGTTCCTGTGCAGTAGAGGCCTTCCACAACCGCGGTGTATTGGATTAATGAGCCTGTTGAATTGGACCATTGTATGAGCCGAAATTCGGTCCAGAACAAGAATAAAATTAATAAAATGAATTCTAATCGATATAATTTGTTAATCCAAATCCAAATCAATGGTTCTAATCCTTATAATAAATATATGAATGATTCTAACACTCCCCTCAAGCTAAAGCTCAATAAGATTGTTACAAGAAAACAATGCCTTTTCCTACAAAACATGAGACCAAGTCTCAACTATCATAAGTTGCCATTGGGATGATCGACAACAGGAAGAGAAGGCGTGGCTAATATGATTCATATGTGGGAAAAAATCGGTCAACGACATCAATTGTTTTTGATGTCTCTGCAACCATATCGTGACTGCAGTTGCGGCCGCATAACCGGTATTTGACCACAATTATCATCATTATGAAGGATATTTACGCGACCATGATTTAAAATTTTGATATTATTTCATACTCTTTCCTGATTCATTATTATCCCTTTCTCCAAATTATAGAATACTGTAATAATTCATCGTGTTCCCTTCACGACCGAAAATCCCCTCTTCTCCACCCGTCGGACGAAGCAAACACAATATGAGAAAAATTAGAAAAGCTTACTTGGCTTAATCATACATATTCATGCATCAGTCAGTAAATAATCACAGACATATCTATAATACAGTGGCTGCCACTAATACACATATCTGATACGCCTTTATGTGGGTATATCATGGTCCATAGTAATCTAGGGTTAGGAATTAAACATTTTTTCTTATTAAACTTGGGTAAATTAAGCATAATTACCTACCAAAAGGACGAAGAAAAAGAGATTCGAAGGGAGAATCCAGGCTATTCAGTAAGAATCGCAGCAATTCCCCAATCCTAACAAAGTAGTTCCTCTGGAATCTAATGAACAACAACGATTACTTAGGATCAGTTTGCGAATTTGTGGCACCGTAGTAGAATCATTACCAATGGCATGTTGTGCGCCTAAGTGCGAAAAGACACTGCAAGCTTTGTTATGCCTGGTTTTGGATTAACTTTTAGCTTATAAGAGCTTCTCAATTAAAATAAGTAATGGTTCTTTACCCCTGAAAAGTTCGTATGCACATATAGATGTCTATGGAGAAGTAAAATAGTATTGATTTTGAAAAAATCGAAATACAGAAGATATTTCAATTTATAGTTTTCTCAGATATAGATTTGCTTGATATTTTTATCCTTCTATATATTCCCCCACTTGTATATTGAACTTTAAACCATTGGTCGAGACAACTACTCTAACCTTTTTCTTGTTCATAAATATAGGTAGTATCTCGGAGGCTGATATACCCCATAACATTGTAGCATTGTTGAAAAGTAAAGCAAAGGGTCATCAAAACCTGAACTCGGTACCTGACAAGCTATCTGCAACTCTGTCTGGACATACTAGAGCTGTCAATGCTATACATTGGTCATCAACTCATGGTAAATTCTCTTAAGTTTACAAGCTGTTGCCCTGTTTGTGATATGGCATACTTTTAGGAAATAATAGTTTTCTTATACTGTTTCTTAATGGTGAAAGTATTGTATTATATATTTTTGCTTGAAGCATGTATTTATTCACAACAAGAGTAAATAAAAAGTTAAAATATTTTAAAAGCATCACACACTCACACAAGTAGGAGAGGATAGACCAGAGCAAGAGATATATCATTGCTCTATATGTTATTAGTAATGATCTGTTCATTGTAGTTACCTTGCAGCTCACCTCCTTGCATCTGCTGGGATGGACAATGTGGTTTTCATATGGAACGTATGGAGCAATGATAAGAAGAAAGCATGTGCGCTAAATTTCCACAATGCAGCAGTGAAAGATGTGAAATGGTCTCAACAAGGGCACTTCTTGCTTTCTTGTGGATATGATTGCACATCAAGGTTGGTTGATGTTGAAAAGGGGATGGAAACTCAAGTTTTCAAAGAAGATCAAATTGTTGGAGTTATAAAGTTCCATCCAGACAATTCAAGTCTCTTTCTTTCTGGGGGATCAAAGGGCCATATCAAGCTATGGGATATCAGAACTGGCAAAGCTGTGCATAACTATAATCGAAGTTTAGGTTCTATTCTAGATGTCGAGTTTACAACAAATGGGAAGCAATTCATGTCTTCAAGTGATGTATCTGGGAGCAATATCAGTGAGAATTCTATTATCGTTTGGGATGTGTCAAGACAGGTACCATTGTCTAATCAGGTGAGATAACTCTACTATTGTTTTACAGTTCACGTAATTTCTGCAAAATTGTTGTGAAAGTTACTTATCATTAGTGTAGGCTGCATTACTGATATTGATTACAACTATTATGTAGCAATAAATTGTTCTAGAGGGAATTTAATAATGGAAATAGCTTAGTAAAAGTCTTCAAAGCTTGCAAGTGCTAGTAACGGATTTAAAAGTTAAAACCAAACTTATAACTGAACCAGTAAATTGACTTATTCTAGATTTAGTGGTTCAACCAACCATGGCTGGATTGTGATTGGATCGGAATCTATGTTTAATATATGACTGGATTGGCCGTTTTAAAAGAATATCAATATTAATATTTTTTTACTAATTTAATACAATTTTCATTTTTCTCTTGAAGCTATATTCTCTTTCATTTAAACTCTGAAATCTAAGATTGTCTCAAGATCCCTAGGTCACCGATAGCCTTGTTCGAAAATTATCCTCCACCACCACCTCTGTTTTAATCTTCTTCTCTGCATAGTCATTCTCCGATCACCAGAGATGTTTCGATCATTATTCCTTATTTCCTGATTTTAATATTAGTGATGTTTTCTATCAAGTCACAGAAGCTTAATGAACATAACTTCCTCTTGTGGGGCTAAGAGTTCAAGTCTATAATCATGGCTCGGACTTCATCGCTCTTTTGCTGCACCACAATCCCTCTACAATTCTGATAGAAAATGGCATCTATATTAGTTCATTACAGTGAAACACTGGAATTTTGTATCAGAAACAGAGAAACGGCAATGTTACAAGATAACACACCAGAATTGAGAAATAACACAAACTAAAGAAAGAGTTAGTTTTCTGTTACAAGGCAACTCTTATCCAAGAAGTTCGAGAGTCTTGGTCTCTCTCTTCGAAGAATTCAAGGAGCTTGGTGTCTCCTAATCAATATCCAGTCCCAAAATAAAATGGCCACTGACTTTTCCTTCCATTTCTGTCTTTATTCAACAACTGTCTAGTACAATAACTGCAGCCTAGTAAGATAATAACATCGGACGCTTCTTTCTTTGTTTCAATCCACTTTTCCATTCAATTGTCCAATATTGTCAAGATCGAGATCTTGCCTAGGTTAGGGAAGGGCGTAGCAAGATCATAAAACATAAGATCATAACGAGGATTGTAAGATCCTACCAAAATAATTACCTAGGATCGGGAGGGGGTTGCAAGATCGTAAAACATAGGATCGTAACAAGGATTGTATCCTACTAAAATGAAAAATTATACTAAAACTGATAGAGAACAGAGGCGAATAATGAAAAACTAACAAAGAAAAATAGCAATGGATCAAATATGGGAATGTACTATTGAAATCAAGGTAATAGATTACAAAAGAAAGAGGAATTCTCTCCTGACTAGAGCACAACTCTTTCAGAGTACTCAGACTCTGCCTGTAACTAACTATAATAGTCTCCAAGTTACCTCTCTCTTGCCTATTCTCTTATAAAAAATTGTTACTCAAAATTCCCTTGCACATGCATTCAGCACTCATCACTCATCCTTTCCTGCCACCTCTGCAGCGGCATTTTCCCCACTCCCTCCTTTTCCACTCTTGACTCTCCTAGTGTAGACCCTCCACACTGCCTGCCTGACTGGGAATTGGTGCATGAGGAGCGTAATCAGCATCTGGGACTATCATTTCCACCTCCTGGAACAGCTAACTTGTCCTCCAATCGGAAAGCTGGGTGTTGACTTTGAATATTGCATGCCTCCTCCCTTCGACTGAATCAACATTTGGCTAACTTTATCTTCTCCCTTTTTAACAGTGTGAGTAGCCAACACTCTCTTAGTTTCCTGAGACTTGGTGTTGTCACCCTCCAAGTCTTGCGGTAACTCCTGCTCCACACAGTAGTTCTTTACTTCTTCGAGAAGACGGCCGTCAAACACTGAATGAACACGGGATGTTTCAGGAAACAGAAGCCTGTAGGCCACCATTCCCAACTTCTCCAAGATTTGCAAAGGGCCACAGAGGAGCCAGCTTCGGGTTGATCGGGCTCCTACAGTGTGCTGTCTGTGAGCTTGCACCTTCATTCTCACTTGTGCCCGTTGTAAATGATGTTTCGGTTGGTGCAGAGCCTCATATCTGTGATGTAGCTCTCTTTTCACCACTTCCACCCTTGTTTCTCCCATTTAAAAATTAGTAACTACCGTAGGCGGCCCCCATAGATTGCTTCAAAATCAGAAGTACCAGTCGATGCATGTTATGTTGTATTATACCAATACTTGGCTCGTGTTAACCACAACACCAAGTCTTTGGCTGGGCCGCAATGAAAACATGAAGAAGGTTTCTAGCATCTATTATTGACCTTAGTCTGCCAAAAAGTAGGTTGTGCTCATCTTTGATTGAGTGCCTTGCAACTTGAACATGTTTTGCCAACAAGTGCTAACCAAAATTGGATCTCTGCCAATACCTTTGCTGTGTCAGGATCATTTAGGGGTTGGAGCAACCCGGCCTGGAGCTGTTTCTAAATACTTCTGGTGTTGGCAAATATCTTATTACCTCACAAACTGCTGGAGAAAATTCTTCATGCCAAACTAGAATAAATTTGCTGCAATCCTCCTATAAGTTCTGTAGAAACCAGAATGTCCCCCGTGGAATGGAGCTCTTTGAGCCAAATAGAAATCCAAGAAAAGTTGGCAGAGCACCATATGACCCTCATATAGCAGCATCTCTGTTCTGCTAAAAGTCATCAATAATCTCATGCAAAACTTTATCCATGTAGACCTCCTCTACCACTTCTTTTCCCTCCAACCAATGGGGATAATTGGTCATAGTACTATGCTCCGAATCTTCATAAATGTGTGATAAAGTATCTGCTCCTCTGTTTTCCAGCCCTGGTTTATACCAAAGTAGCTTTGCTGCCCAGTTCTGCTGATCCACTGTGGTAATCTTCTGCAGCAAAAATTGTTTTGACAGTAAATTGTTTTCGCCAAATTTCTTTCTCCTGATGTCTTGCTATAATAAGCAACGGGTCTGCCATTTTGGGTGAGAATATCCACCAACCCTTTCCCTGATACGTCATCACATTTGATAACAAATTCCTGACTGAAATCAGGCAGCCTTAGAACCGGTGCCATAGTCATTTTCTTTTTTTATCACATCAAAAGCTTTTTGAGCTCGAGCCCCTCATTTAAAGGCTTCAATTTTTGCGAGCGCCGTCAGTGGCTTGGCAAATTTTCAATAGTCTTTGATGACTTTTCGATAATACACGATTAATCCCACACCCCTTTGATGTTCTTAGGGTGTGTGTGCCCAATGTCACACTTACCTTATTGGGGTCCATTGCAACCCCTTTGATGTATATAATGTGCCCGATGTATTCCAAAGAAGTTGGAGCAAAATCACACATGTTCTTATTGGATACAAGTTGCTGTCACATCCTACTATCACATATTCTTAATAAATAACTGCTATAATTTTTATTCATAACTGCCATTAAACTGTTTTGTCGCTAATTATTTCCTAACTACCGCCATCAAGTAATTCTACCACTTATTATTTCCTAATTGGAACTGCCATCCATACTGTTTCCAATGCAGCCTAGTATACACGTTCTAAATGGGTGGCTTGTTGGTTTATAGACCAAGGGATCTGCCTCTTAGAATCAGAGTGCATAATTGGGAGATTGTTGGATTCAGAGTCAGAGAGACTGGGGTGTCTTTTGACATGTTTATAGGCTAAGGGACTAGTTACAACTTGACACTCACCAAACCAAACACTGTTGGGACACCCATTCGACACCTTTAACTCCCCAACTATGACTTGACACATAAGTAAACACGGATCTTGTAAAAAAAAACCTTCCGTTATGTTTTTAAATAACTTGGATAAATGTTGGCATGAAATATAGACAAGAAATATTAAATAATGTTAAGAAAGATCTCACATAGACTCCAGTATGTTGTCCGTTCACCTGTTCATCCTATGTCTCCCACACGTGTATATATGTTGGAGGCTTATCAAGTGGGTACGTAAGAGGAGATAATCTTGATCCTATTCCTGTACTTGAATGCTCCTGTTATATGCTTCACAAACGTCAGTTCCCGGCGTCCTATATTTAGACACTGCTCATGTTTGTGTCATTTCTATGGTCCATGTTTATGTTAGTATTTAATCGGTTCCAACCCAGAAAAATGAGTAAAAATAGCAGCACATTACATTAACAGCAAACAAAAAGACCTCTTTGGCAGCAACGGAGGCAGCTAATTGTGGTTCTGGGTTGCACTGATGAATAGAAAACACATTGATGGTCCTAAAAACAAAGACACAACCCAAAAACACTTGCAACGAAGGGACTCTGCCGTTTTGTTAACCATCTACATATCAGATTAATCTACCAAAATTTTTATCTACATTTTATATTTATGTAAACTTGTCTTTGCTCGAGGGACTCTGTCGTTTTGTTGAATATTGAGATTGGTTGATGGGTGCAAGGTAATAATATTCATGCAGAACTCACATTTCTAAGAAGATTAATAAGTTTGTTAAATTTAAACATGATCTGCAGTAATAACAAATATTTTGAAAGGTCATTTTTTACCTCTTCTGAAGACTTTTATCTAATATTAATCTGCAGTAATAACAAATATTTTGAAACGTCATTTTTCACCTCTTCTGAAGACTTTTATCTAATATTAATGCTGAGATAAAACTCGACACAGAAGTGCTAATGGTGCTATATGCATTCTAGAGTGTGCAGTTATTAATATCAATTTGACCATATATATGAATAAACTTTGTTAAGACTTTTAGTATAAATTTTAATTAACATGTTATGCTTCTGAATCAGGTTTATGTGGAAGCTTATACTTGTCCTTGCATTAGAATTCACCCCTTTGATCCAGTTTTTGTCGCGCAATCAAACGGGAATTATGTTGCTATCTTTAGTACCTACCCACCATACAGACTCAACAAATATAAGAGATATGAGAACCATGGGGTTTCTGGGTTCCCAATTAAGTGCAATTTCAGCTTAGATGGGAAGAAGCTTGCATCTGGCTCTTCAGATGGATCTATTTACCTTTATGATTATCAGTCATCCAAAGTTTTGAAGAAAATAAAGGCCTTTAACCAAGCATGCATGGACATTGCCTTCCACCCAGTTTTGCCTAATGTTATTGCTTCATGCTCATGGGATGGAAGTATTTTGGTATTTGAGTAG
Protein sequence

>MS.gene25515.t1

MTIWCSSTVGKKPAKQLTTPMLNIVCELEREKCEKMREVAWSYVYIAELSVTASEIQRRMRELEIGSTIRICPGRCSCAVEAFHNRGSISEADIPHNIVALLKSKAKGHQNLNSVPDKLSATLSGHTRAVNAIHWSSTHAHLLASAGMDNVVFIWNVWSNDKKKACALNFHNAAVKDVKWSQQGHFLLSCGYDCTSRLVDVEKGMETQVFKEDQIVGVIKFHPDNSSLFLSGGSKGHIKLWDIRTGKAVHNYNRSLGSILDVEFTTNGKQFMSSSDVSGSNISENSIIVWDVSRQVPLSNQVYVEAYTCPCIRIHPFDPVFVAQSNGNYVAIFSTYPPYRLNKYKRYENHGVSGFPIKCNFSLDGKKLASGSSDGSIYLYDYQSSKVLKKIKAFNQACMDIAFHPVLPNVIASCSWDGSILVFE