AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31117


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31117.t1 MTR_2g097680 92.398 171 7 4 1 170 18 183 5.09e-80 235
MS.gene31117.t1 MTR_1g071380 90.643 171 8 6 1 170 1 164 8.29e-77 226
MS.gene31117.t1 MTR_2g097680 89.831 118 6 4 1 117 18 130 2.81e-57 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31117.t1 AT2G29180 41.772 158 75 5 20 163 5 159 4.46e-24 92.8

Find 29 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTATCCGTTATCACTAT+TGG 0.245133 2.4:+6188987 MS.gene31117:CDS
GCTTATCCGTTATCACTATT+GGG 0.260636 2.4:+6188988 MS.gene31117:CDS
TCTGGTTGTAGTAGTGATAA+TGG 0.300412 2.4:-6188793 None:intergenic
GAAAGGAAGGTTGCAATCTT+TGG 0.300675 2.4:-6188696 None:intergenic
GCTGTAGCAGCAACAGGATT+TGG 0.334339 2.4:-6188852 None:intergenic
TTCAAAGGAAGTCCAAACTA+TGG 0.343791 2.4:+6188829 MS.gene31117:CDS
CATTGATGCTTCGGAGCAAT+TGG 0.360695 2.4:+6188896 MS.gene31117:CDS
CTGGTTGTAGTAGTGATAAT+GGG 0.369858 2.4:-6188792 None:intergenic
AACAGGATTTGGCCATAGTT+TGG 0.372494 2.4:-6188841 None:intergenic
AAAAGAAAAGAAAAGTAGTA+AGG 0.384781 2.4:+6189988 MS.gene31117:CDS
AGGGCAGGACCTAAAGGATT+TGG 0.404020 2.4:+6190008 MS.gene31117:CDS
GAGAAAGATGAAGAAGTTGT+TGG 0.423658 2.4:-6188768 None:intergenic
TGCTACAGCCATTGATGCTT+CGG 0.431580 2.4:+6188887 MS.gene31117:CDS
TCAACAGCTGTAGCAGCAAC+AGG 0.437132 2.4:-6188858 None:intergenic
CAATTGCTCCGAAGCATCAA+TGG 0.447559 2.4:-6188895 None:intergenic
AGAAGATGATGTTGAAAGGA+AGG 0.478894 2.4:-6188709 None:intergenic
TCATCAACTACCACTGATGA+TGG 0.480753 2.4:+6188921 MS.gene31117:CDS
AAAGAAAAGAAAAGTAGTAA+GGG 0.491413 2.4:+6189989 MS.gene31117:CDS
AAGAAGAAGATGATGTTGAA+AGG 0.496918 2.4:-6188713 None:intergenic
GTAGTAAGGGCAGGACCTAA+AGG 0.510055 2.4:+6190002 MS.gene31117:CDS
CTTATCCGTTATCACTATTG+GGG 0.525510 2.4:+6188989 MS.gene31117:CDS
CATCAACTACCACTGATGAT+GGG 0.525641 2.4:+6188922 MS.gene31117:CDS
AAAAGAAAAGTAGTAAGGGC+AGG 0.536130 2.4:+6189993 MS.gene31117:CDS
ATATACCCCAATAGTGATAA+CGG 0.550650 2.4:-6188994 None:intergenic
TTTCTTGACAAAGAGAGAGA+AGG 0.571247 2.4:+6189926 MS.gene31117:CDS
ATCAACTACCACTGATGATG+GGG 0.608198 2.4:+6188923 MS.gene31117:CDS
AAGAGACACCCCATCATCAG+TGG 0.647668 2.4:-6188931 None:intergenic
GAGAAAGAAGAAGCAGCTAG+CGG 0.687353 2.4:+6189957 MS.gene31117:CDS
TGATGATGCAAATAATGCAG+TGG 0.718262 2.4:-6188664 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCTATATTAATAAAAGAAT+TGG - chr2.4:6189054-6189073 None:intergenic 15.0%
!! TATTCAAAATGAACTAATTA+GGG - chr2.4:6189413-6189432 None:intergenic 15.0%
!!! AAATGATTATGAACTAATTT+TGG - chr2.4:6189386-6189405 None:intergenic 15.0%
!!! AATAGAATTTTACAATATTG+AGG + chr2.4:6189429-6189448 MS.gene31117:intron 15.0%
!!! CAATTCTTTTATTAATATAG+AGG + chr2.4:6189052-6189071 MS.gene31117:intron 15.0%
!!! TTTTATAAATGTAAATGACT+TGG + chr2.4:6189774-6189793 MS.gene31117:intron 15.0%
!!! TTTTTCGTTTTGTAATTTTA+TGG + chr2.4:6189633-6189652 MS.gene31117:intron 15.0%
!! AAAAGAAAAGAAAAGTAGTA+AGG + chr2.4:6189988-6190007 MS.gene31117:CDS 20.0%
!! AAAGAAAAGAAAAGTAGTAA+GGG + chr2.4:6189989-6190008 MS.gene31117:CDS 20.0%
!! CTAAATTGATTATGAAAGTT+AGG - chr2.4:6189327-6189346 None:intergenic 20.0%
!! CTATTCAAAATGAACTAATT+AGG - chr2.4:6189414-6189433 None:intergenic 20.0%
!!! GAAAATCCATAATTTTTCAA+AGG + chr2.4:6188814-6188833 MS.gene31117:CDS 20.0%
!!! TATTGACATTACATGTTTTA+GGG + chr2.4:6189246-6189265 MS.gene31117:intron 20.0%
!!! TTATTGACATTACATGTTTT+AGG + chr2.4:6189245-6189264 MS.gene31117:intron 20.0%
!!! TTGAAAAATTATGGATTTTC+TGG - chr2.4:6188814-6188833 None:intergenic 20.0%
!!! TTGCGATTTTAATACATTAA+GGG - chr2.4:6189597-6189616 None:intergenic 20.0%
!!! TTTGCGATTTTAATACATTA+AGG - chr2.4:6189598-6189617 None:intergenic 20.0%
! TTACTTAGCATTAGTATTCA+GGG + chr2.4:6189848-6189867 MS.gene31117:intron 25.0%
! TTAGTGAAAATTATTTCCCT+TGG + chr2.4:6189162-6189181 MS.gene31117:intron 25.0%
!! TTTTGATCTTTGAATATGTG+AGG - chr2.4:6189740-6189759 None:intergenic 25.0%
AAGAAGAAGATGATGTTGAA+AGG - chr2.4:6188716-6188735 None:intergenic 30.0%
AGTAAAAACGTGTGTTGAAA+TGG - chr2.4:6188743-6188762 None:intergenic 30.0%
ATATACCCCAATAGTGATAA+CGG - chr2.4:6188997-6189016 None:intergenic 30.0%
CACATATTTAGAGACCTAAA+TGG - chr2.4:6189556-6189575 None:intergenic 30.0%
CTAGTTTATACTTTGTAGTG+TGG - chr2.4:6189220-6189239 None:intergenic 30.0%
GTTACTTAGCATTAGTATTC+AGG + chr2.4:6189847-6189866 MS.gene31117:intron 30.0%
TTTAGGTCTCTAAATATGTG+AGG + chr2.4:6189556-6189575 MS.gene31117:intron 30.0%
! ATCTTTTGACCAAATCCTTT+AGG - chr2.4:6190020-6190039 None:intergenic 30.0%
! CCTCCTATCTAAAACTATTT+CGG + chr2.4:6189823-6189842 MS.gene31117:intron 30.0%
! CTTTTCTTCATTGCTTTCAT+TGG + chr2.4:6188966-6188985 MS.gene31117:CDS 30.0%
! GGACTTCCTTTGAAAAATTA+TGG - chr2.4:6188823-6188842 None:intergenic 30.0%
! TTTAAAGCAAACAGCCATTT+AGG + chr2.4:6189539-6189558 MS.gene31117:intron 30.0%
!! TTTTTGTTTGTTTGTGTTGC+AGG + chr2.4:6189881-6189900 MS.gene31117:intron 30.0%
!!! ACACCGAAATAGTTTTAGAT+AGG - chr2.4:6189829-6189848 None:intergenic 30.0%
AAAAGAAAAGTAGTAAGGGC+AGG + chr2.4:6189993-6190012 MS.gene31117:CDS 35.0%
AGAAGATGATGTTGAAAGGA+AGG - chr2.4:6188712-6188731 None:intergenic 35.0%
ATTCCATGTCATATTGTCCA+AGG - chr2.4:6189182-6189201 None:intergenic 35.0%
CTGGTTGTAGTAGTGATAAT+GGG - chr2.4:6188795-6188814 None:intergenic 35.0%
CTTATCCGTTATCACTATTG+GGG + chr2.4:6188989-6189008 MS.gene31117:CDS 35.0%
GAGAAAGATGAAGAAGTTGT+TGG - chr2.4:6188771-6188790 None:intergenic 35.0%
GCTTATCCGTTATCACTATT+GGG + chr2.4:6188988-6189007 MS.gene31117:CDS 35.0%
TCTGGTTGTAGTAGTGATAA+TGG - chr2.4:6188796-6188815 None:intergenic 35.0%
TTCAAAGGAAGTCCAAACTA+TGG + chr2.4:6188829-6188848 MS.gene31117:CDS 35.0%
TTCCATGTCATATTGTCCAA+GGG - chr2.4:6189181-6189200 None:intergenic 35.0%
TTGAGACATGCTTGCAATTT+CGG - chr2.4:6189089-6189108 None:intergenic 35.0%
TTTCTTGACAAAGAGAGAGA+AGG + chr2.4:6189926-6189945 MS.gene31117:CDS 35.0%
! TGATGATGCAAATAATGCAG+TGG - chr2.4:6188667-6188686 None:intergenic 35.0%
!!! CCGAAATAGTTTTAGATAGG+AGG - chr2.4:6189826-6189845 None:intergenic 35.0%
AACAGGATTTGGCCATAGTT+TGG - chr2.4:6188844-6188863 None:intergenic 40.0%
AATCGCAAACACATCTCTAG+TGG + chr2.4:6189609-6189628 MS.gene31117:intron 40.0%
ACACATGCAGTCAACTTACT+AGG - chr2.4:6189352-6189371 None:intergenic 40.0%
CACATGCAGTCAACTTACTA+GGG - chr2.4:6189351-6189370 None:intergenic 40.0%
CATCAACTACCACTGATGAT+GGG + chr2.4:6188922-6188941 MS.gene31117:CDS 40.0%
CTTAGCATTAGTATTCAGGG+AGG + chr2.4:6189851-6189870 MS.gene31117:intron 40.0%
GAAAGGAAGGTTGCAATCTT+TGG - chr2.4:6188699-6188718 None:intergenic 40.0%
GGCAAATGGTCACGATTTAA+AGG + chr2.4:6189489-6189508 MS.gene31117:intron 40.0%
GGCTTATCCGTTATCACTAT+TGG + chr2.4:6188987-6189006 MS.gene31117:CDS 40.0%
TCATCAACTACCACTGATGA+TGG + chr2.4:6188921-6188940 MS.gene31117:CDS 40.0%
TTCCCTTGGACAATATGACA+TGG + chr2.4:6189176-6189195 MS.gene31117:intron 40.0%
! AGTAAGTTGACTGCATGTGT+TGG + chr2.4:6189352-6189371 MS.gene31117:intron 40.0%
! ATCAACTACCACTGATGATG+GGG + chr2.4:6188923-6188942 MS.gene31117:CDS 40.0%
CATTGATGCTTCGGAGCAAT+TGG + chr2.4:6188896-6188915 MS.gene31117:CDS 45.0%
GAGAAAGAAGAAGCAGCTAG+CGG + chr2.4:6189957-6189976 MS.gene31117:CDS 45.0%
TGCTACAGCCATTGATGCTT+CGG + chr2.4:6188887-6188906 MS.gene31117:CDS 45.0%
! CAATTGCTCCGAAGCATCAA+TGG - chr2.4:6188898-6188917 None:intergenic 45.0%
! GCCAAAACGAGTGACTAGTT+TGG + chr2.4:6189468-6189487 MS.gene31117:intron 45.0%
! GCCAAACTAGTCACTCGTTT+TGG - chr2.4:6189472-6189491 None:intergenic 45.0%
!! CGAGTGACTAGTTTGGCAAA+TGG + chr2.4:6189475-6189494 MS.gene31117:intron 45.0%
AAGAGACACCCCATCATCAG+TGG - chr2.4:6188934-6188953 None:intergenic 50.0%
AGGGCAGGACCTAAAGGATT+TGG + chr2.4:6190008-6190027 MS.gene31117:CDS 50.0%
GCTGTAGCAGCAACAGGATT+TGG - chr2.4:6188855-6188874 None:intergenic 50.0%
GTAGTAAGGGCAGGACCTAA+AGG + chr2.4:6190002-6190021 MS.gene31117:CDS 50.0%
TCAACAGCTGTAGCAGCAAC+AGG - chr2.4:6188861-6188880 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6188660 6190064 6188660 ID=MS.gene31117
chr2.4 mRNA 6188660 6190064 6188660 ID=MS.gene31117.t1;Parent=MS.gene31117
chr2.4 exon 6188660 6189010 6188660 ID=MS.gene31117.t1.exon1;Parent=MS.gene31117.t1
chr2.4 CDS 6188660 6189010 6188660 ID=cds.MS.gene31117.t1;Parent=MS.gene31117.t1
chr2.4 exon 6189903 6190064 6189903 ID=MS.gene31117.t1.exon2;Parent=MS.gene31117.t1
chr2.4 CDS 6189903 6190064 6189903 ID=cds.MS.gene31117.t1;Parent=MS.gene31117.t1
Gene Sequence

>MS.gene31117

ATGGCCACTGCATTATTTGCATCATCATCATCATCTCCAAAGATTGCAACCTTCCTTTCAACATCATCTTCTTCTTCTTTCCATTTCAACACACGTTTTTACTCACTTCCAACAACTTCTTCATCTTTCTCTCCCATTATCACTACTACAACCAGAAAATCCATAATTTTTCAAAGGAAGTCCAAACTATGGCCAAATCCTGTTGCTGCTACAGCTGTTGAAGATGCTGCTACAGCCATTGATGCTTCGGAGCAATTGGTTTCATCAACTACCACTGATGATGGGGTGTCTCTTATCGTATCAGCTCTTTTCTTCATTGCTTTCATTGGCTTATCCGTTATCACTATTGGGGTATATTCGTCATTTTCTTCTATTCTCACTTTTTTGTTTTCCAATTCTTTTATTAATATAGAGGTAAATTATCATCCGAAATTGCAAGCATGTCTCAAAATAGTGTTTGATTTTTGTGAATTGACAAACATATTTGTGAAATTGTAAAATGTTAGTGAAAATTATTTCCCTTGGACAATATGACATGGAATCTAACATCAATGTTACCACACTACAAAGTATAAACTAGTTTGTTTATTGACATTACATGTTTTAGGGACTAATTTGTCAATAATTAATTAGTTCATGTATATTTGTAATTATTGATAAATTACCTAACTTTCATAATCAATTTAGTCCCTAGTAAGTTGACTGCATGTGTTGGATCAACGTCCAAAATTAGTTCATAATCATTTTAGTCCCTAATTAGTTCATTTTGAATAGAATTTTACAATATTGAGGATGTATATGTCAATTCGCCAAAACGAGTGACTAGTTTGGCAAATGGTCACGATTTAAAGGATCAATTTGACTATGCGCACTCATTTGTTTAAAGCAAACAGCCATTTAGGTCTCTAAATATGTGAGGTGTTGTTAGTACAGTCCCTTAATGTATTAAAATCGCAAACACATCTCTAGTGGCTTTTTCGTTTTGTAATTTTATGGATTATATTGACTAACGAATAAAAAAAAAAAACATGTTGATATGCTTGTAATTCGATTCACTCAGTGACTATAGTGACAACACCTCACATATTCAAAGATCAAAATGATTGATTATCGTTTTTATAAATGTAAATGACTTGGTAAGTTAGTAGTATTTGAACTTTTGACCTCCTATCTAAAACTATTTCGGTGTTACTTAGCATTAGTATTCAGGGAGGTTCTGTATTTTTGTTTGTTTGTGTTGCAGGTTATCTATTTAGCAGTAACAGATTTCTTGACAAAGAGAGAGAAGGAAAAATTTGAGAAAGAAGAAGCAGCTAGCGGAAAGAAGAAAAAGAAAAGAAAAGTAGTAAGGGCAGGACCTAAAGGATTTGGTCAAAAGATTGTTCAAACTGAAGATGATGATTGA

Protein sequence

>MS.gene31117.t1

MATALFASSSSSPKIATFLSTSSSSSFHFNTRFYSLPTTSSSFSPIITTTTRKSIIFQRKSKLWPNPVAATAVEDAATAIDASEQLVSSTTTDDGVSLIVSALFFIAFIGLSVITIGVIYLAVTDFLTKREKEKFEKEEAASGKKKKKRKVVRAGPKGFGQKIVQTEDDD