Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97055.t1 | AFK42793.1 | 99 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 4.90E-148 | 533.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97055.t1 | Q84X53 | 35.9 | 237 | 151 | 1 | 56 | 291 | 33 | 269 | 2.6e-33 | 144.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97055.t1 | G7IIY0 | 99.0 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 3.5e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene97055.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene97055 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene049173 | MS.gene97055 | 0.813894 | 2.03E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene97055 | 0.828299 | 9.71E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene97055 | 0.820629 | 6.20E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene97055 | 0.80077 | 1.23E-48 | -1.69E-46 |
| MS.gene049320 | MS.gene97055 | 0.833089 | 6.52E-56 | -1.69E-46 |
| MS.gene049356 | MS.gene97055 | 0.811326 | 7.40E-51 | -1.69E-46 |
| MS.gene049369 | MS.gene97055 | 0.831216 | 1.89E-55 | -1.69E-46 |
| MS.gene049427 | MS.gene97055 | 0.836737 | 7.85E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene97055 | 0.855174 | 7.53E-62 | -1.69E-46 |
| MS.gene049661 | MS.gene97055 | 0.837902 | 3.95E-57 | -1.69E-46 |
| MS.gene049662 | MS.gene97055 | 0.821364 | 4.20E-53 | -1.69E-46 |
| MS.gene049760 | MS.gene97055 | 0.817472 | 3.24E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene97055 | 0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene97055 | 0.821132 | 4.75E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene97055 | 0.814624 | 1.40E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene97055 | 0.869799 | 2.36E-66 | -1.69E-46 |
| MS.gene050593 | MS.gene97055 | 0.81022 | 1.28E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene97055 | 0.811381 | 7.20E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene97055 | 0.808614 | 2.84E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene97055 | 0.847221 | 1.33E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene97055 | 0.847347 | 1.23E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene97055 | 0.868712 | 5.32E-66 | -1.69E-46 |
| MS.gene050950 | MS.gene97055 | 0.806526 | 7.88E-50 | -1.69E-46 |
| MS.gene05100 | MS.gene97055 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene97055 | 0.814117 | 1.81E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene97055 | 0.859341 | 4.42E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene97055 | 0.843714 | 1.18E-58 | -1.69E-46 |
| MS.gene051666 | MS.gene97055 | 0.820262 | 7.53E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene97055 | 0.807874 | 4.08E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene97055 | 0.828162 | 1.05E-54 | -1.69E-46 |
| MS.gene051858 | MS.gene97055 | 0.846327 | 2.33E-59 | -1.69E-46 |
| MS.gene052271 | MS.gene97055 | 0.826602 | 2.48E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene97055 | 0.814948 | 1.19E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene97055 | 0.819234 | 1.29E-52 | -1.69E-46 |
| MS.gene052322 | MS.gene97055 | 0.807986 | 3.87E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene97055 | 0.825893 | 3.66E-54 | -1.69E-46 |
| MS.gene052481 | MS.gene97055 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene97055 | 0.82138 | 4.16E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene97055 | 0.827029 | 1.96E-54 | -1.69E-46 |
| MS.gene053009 | MS.gene97055 | 0.877956 | 4.14E-69 | -1.69E-46 |
| MS.gene053103 | MS.gene97055 | 0.815775 | 7.78E-52 | -1.69E-46 |
| MS.gene053104 | MS.gene97055 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene97055 | 0.867579 | 1.23E-65 | -1.69E-46 |
| MS.gene053209 | MS.gene97055 | 0.859095 | 5.24E-63 | -1.69E-46 |
| MS.gene053213 | MS.gene97055 | 0.847681 | 9.91E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene97055 | 0.839353 | 1.67E-57 | -1.69E-46 |
| MS.gene053322 | MS.gene97055 | 0.830926 | 2.23E-55 | -1.69E-46 |
| MS.gene053574 | MS.gene97055 | 0.83742 | 5.25E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene97055 | 0.844615 | 6.78E-59 | -1.69E-46 |
| MS.gene053912 | MS.gene97055 | 0.823288 | 1.50E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene97055 | 0.824337 | 8.52E-54 | -1.69E-46 |
| MS.gene054376 | MS.gene97055 | 0.847305 | 1.26E-59 | -1.69E-46 |
| MS.gene054600 | MS.gene97055 | 0.834035 | 3.78E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene97055 | 0.834755 | 2.50E-56 | -1.69E-46 |
| MS.gene054802 | MS.gene97055 | 0.802045 | 6.76E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene97055 | 0.806592 | 7.63E-50 | -1.69E-46 |
| MS.gene054927 | MS.gene97055 | 0.848944 | 4.44E-60 | -1.69E-46 |
| MS.gene05505 | MS.gene97055 | 0.846799 | 1.73E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene97055 | 0.846145 | 2.61E-59 | -1.69E-46 |
| MS.gene055206 | MS.gene97055 | 0.828711 | 7.73E-55 | -1.69E-46 |
| MS.gene055212 | MS.gene97055 | 0.800836 | 1.20E-48 | -1.69E-46 |
| MS.gene055254 | MS.gene97055 | 0.858628 | 7.23E-63 | -1.69E-46 |
| MS.gene055277 | MS.gene97055 | 0.800723 | 1.26E-48 | -1.69E-46 |
| MS.gene055289 | MS.gene97055 | 0.826787 | 2.24E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene97055 | 0.830048 | 3.66E-55 | -1.69E-46 |
| MS.gene055397 | MS.gene97055 | 0.841299 | 5.17E-58 | -1.69E-46 |
| MS.gene055499 | MS.gene97055 | 0.857097 | 2.06E-62 | -1.69E-46 |
| MS.gene055647 | MS.gene97055 | 0.821216 | 4.54E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene97055 | 0.883986 | 2.82E-71 | -1.69E-46 |
| MS.gene05592 | MS.gene97055 | 0.831396 | 1.71E-55 | -1.69E-46 |
| MS.gene056083 | MS.gene97055 | 0.854078 | 1.56E-61 | -1.69E-46 |
| MS.gene056191 | MS.gene97055 | 0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene97055 | 0.823117 | 1.65E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene97055 | 0.813018 | 3.16E-51 | -1.69E-46 |
| MS.gene056487 | MS.gene97055 | 0.822009 | 2.98E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene97055 | 0.834585 | 2.75E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene97055 | 0.841246 | 5.34E-58 | -1.69E-46 |
| MS.gene05664 | MS.gene97055 | 0.812543 | 4.02E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene97055 | 0.827637 | 1.40E-54 | -1.69E-46 |
| MS.gene05666 | MS.gene97055 | 0.803848 | 2.87E-49 | -1.69E-46 |
| MS.gene056897 | MS.gene97055 | 0.803655 | 3.14E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene97055 | 0.804201 | 2.42E-49 | -1.69E-46 |
| MS.gene057048 | MS.gene97055 | 0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene97055 | 0.826641 | 2.43E-54 | -1.69E-46 |
| MS.gene057129 | MS.gene97055 | 0.860632 | 1.80E-63 | -1.69E-46 |
| MS.gene057146 | MS.gene97055 | 0.835494 | 1.62E-56 | -1.69E-46 |
| MS.gene057331 | MS.gene97055 | 0.841468 | 4.66E-58 | -1.69E-46 |
| MS.gene05735 | MS.gene97055 | 0.812667 | 3.78E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene97055 | 0.851177 | 1.05E-60 | -1.69E-46 |
| MS.gene057734 | MS.gene97055 | 0.81435 | 1.61E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene97055 | 0.806852 | 6.73E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene97055 | 0.816857 | 4.45E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene97055 | 0.803079 | 4.14E-49 | -1.69E-46 |
| MS.gene058130 | MS.gene97055 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene058419 | MS.gene97055 | 0.828324 | 9.58E-55 | -1.69E-46 |
| MS.gene058459 | MS.gene97055 | 0.800427 | 1.45E-48 | -1.69E-46 |
| MS.gene058660 | MS.gene97055 | 0.827738 | 1.33E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene97055 | 0.88416 | 2.43E-71 | -1.69E-46 |
| MS.gene058773 | MS.gene97055 | 0.81111 | 8.25E-51 | -1.69E-46 |
| MS.gene05883 | MS.gene97055 | 0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene97055 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene97055 | 0.816152 | 6.41E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene97055 | 0.813564 | 2.40E-51 | -1.69E-46 |
| MS.gene059536 | MS.gene97055 | 0.833069 | 6.59E-56 | -1.69E-46 |
| MS.gene059597 | MS.gene97055 | 0.8006 | 1.34E-48 | -1.69E-46 |
| MS.gene059677 | MS.gene97055 | 0.823326 | 1.47E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene97055 | 0.838287 | 3.14E-57 | -1.69E-46 |
| MS.gene059692 | MS.gene97055 | 0.845246 | 4.58E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene97055 | 0.84116 | 5.62E-58 | -1.69E-46 |
| MS.gene059711 | MS.gene97055 | 0.847959 | 8.31E-60 | -1.69E-46 |
| MS.gene059718 | MS.gene97055 | 0.84573 | 3.38E-59 | -1.69E-46 |
| MS.gene05986 | MS.gene97055 | 0.805496 | 1.30E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene97055 | 0.848265 | 6.84E-60 | -1.69E-46 |
| MS.gene059960 | MS.gene97055 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene060356 | MS.gene97055 | 0.824012 | 1.02E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene97055 | 0.804222 | 2.40E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene97055 | 0.81573 | 7.96E-52 | -1.69E-46 |
| MS.gene060469 | MS.gene97055 | 0.818128 | 2.30E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene97055 | 0.854696 | 1.04E-61 | -1.69E-46 |
| MS.gene060818 | MS.gene97055 | 0.832356 | 9.90E-56 | -1.69E-46 |
| MS.gene060835 | MS.gene97055 | 0.828681 | 7.85E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene97055 | 0.835171 | 1.96E-56 | -1.69E-46 |
| MS.gene061041 | MS.gene97055 | 0.849142 | 3.91E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene97055 | -0.801954 | 7.06E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene97055 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene061200 | MS.gene97055 | 0.842436 | 2.59E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97055 | MS.gene77650 | PPI |
| MS.gene97055 | MS.gene039043 | PPI |
| MS.gene92189 | MS.gene97055 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97055.t1 | MTR_2g049780 | 99.000 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 0.0 | 603 |
| MS.gene97055.t1 | MTR_5g041630 | 42.491 | 273 | 151 | 1 | 30 | 296 | 25 | 297 | 3.68e-68 | 214 |
| MS.gene97055.t1 | MTR_2g067230 | 39.076 | 238 | 140 | 2 | 56 | 293 | 69 | 301 | 4.93e-57 | 187 |
| MS.gene97055.t1 | MTR_7g081270 | 40.654 | 214 | 123 | 4 | 81 | 291 | 67 | 279 | 2.27e-42 | 147 |
| MS.gene97055.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 322 | 549 | 2.18e-25 | 106 |
| MS.gene97055.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 261 | 488 | 2.20e-25 | 105 |
| MS.gene97055.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 326 | 553 | 2.27e-25 | 105 |
| MS.gene97055.t1 | MTR_4g070060 | 28.112 | 249 | 170 | 6 | 55 | 296 | 344 | 590 | 1.80e-21 | 94.7 |
| MS.gene97055.t1 | MTR_5g068860 | 26.136 | 264 | 154 | 7 | 69 | 293 | 223 | 484 | 1.75e-19 | 89.0 |
| MS.gene97055.t1 | MTR_6g003960 | 25.000 | 228 | 159 | 3 | 70 | 291 | 366 | 587 | 5.80e-18 | 84.3 |
| MS.gene97055.t1 | MTR_5g094610 | 24.215 | 223 | 154 | 4 | 57 | 268 | 227 | 445 | 1.66e-17 | 82.8 |
| MS.gene97055.t1 | MTR_4g084630 | 23.600 | 250 | 181 | 4 | 52 | 291 | 69 | 318 | 3.86e-17 | 80.9 |
| MS.gene97055.t1 | MTR_1g115415 | 25.556 | 270 | 160 | 5 | 63 | 293 | 185 | 452 | 4.93e-17 | 81.6 |
| MS.gene97055.t1 | MTR_0320s0040 | 35.556 | 90 | 57 | 1 | 56 | 145 | 48 | 136 | 2.92e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97055.t1 | AT2G34620 | 66.212 | 293 | 92 | 3 | 9 | 295 | 3 | 294 | 1.84e-129 | 370 |
| MS.gene97055.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 32 | 268 | 7.64e-70 | 218 |
| MS.gene97055.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 33 | 269 | 9.57e-70 | 218 |
| MS.gene97055.t1 | AT2G36000 | 37.037 | 243 | 144 | 4 | 58 | 296 | 75 | 312 | 4.31e-51 | 171 |
| MS.gene97055.t1 | AT2G36000 | 37.037 | 243 | 144 | 4 | 58 | 296 | 75 | 312 | 5.56e-51 | 171 |
| MS.gene97055.t1 | AT3G18870 | 37.705 | 244 | 143 | 4 | 51 | 291 | 31 | 268 | 5.91e-46 | 156 |
| MS.gene97055.t1 | AT2G21710 | 28.340 | 247 | 168 | 6 | 57 | 296 | 353 | 597 | 3.38e-24 | 102 |
| MS.gene97055.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 1.07e-21 | 95.1 |
| MS.gene97055.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 1.07e-21 | 95.1 |
| MS.gene97055.t1 | AT1G78930 | 25.328 | 229 | 167 | 2 | 65 | 291 | 348 | 574 | 1.59e-19 | 89.0 |
| MS.gene97055.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 226 | 443 | 7.02e-17 | 81.3 |
| MS.gene97055.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 120 | 337 | 7.55e-17 | 80.5 |
| MS.gene97055.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 4.46e-16 | 79.0 |
| MS.gene97055.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 4.46e-16 | 79.0 |
| MS.gene97055.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.17e-15 | 76.6 |
| MS.gene97055.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.17e-15 | 76.6 |
| MS.gene97055.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.60e-15 | 76.6 |
| MS.gene97055.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.79e-15 | 76.3 |
| MS.gene97055.t1 | AT2G44020 | 25.481 | 208 | 119 | 2 | 86 | 293 | 286 | 457 | 3.95e-15 | 75.9 |
| MS.gene97055.t1 | AT2G21710 | 25.243 | 206 | 145 | 6 | 57 | 255 | 353 | 556 | 3.29e-13 | 70.1 |
| MS.gene97055.t1 | AT2G21710 | 24.878 | 205 | 145 | 6 | 57 | 254 | 353 | 555 | 2.52e-12 | 67.4 |
Find 57 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTAAGCATTTGGAGAGTA+TGG | 0.202455 | 2.2:+37499489 | MS.gene97055:CDS |
| AAAGTACTGATGATGAATTT+AGG | 0.234140 | 2.2:+37499749 | MS.gene97055:CDS |
| TTATCAAGATTGTGTTCTTT+TGG | 0.240932 | 2.2:+37499435 | MS.gene97055:CDS |
| TTTGGGTTTGGGAGTTAAAT+TGG | 0.262027 | 2.2:-37498981 | None:intergenic |
| GAAGTAATGGGCATTGATTC+AGG | 0.276282 | 2.2:+37499085 | MS.gene97055:CDS |
| ATGCTTGATGTGAGAATCTT+AGG | 0.276341 | 2.2:-37499232 | None:intergenic |
| TGTGGAAATGAAGGGGAAAT+TGG | 0.285794 | 2.2:+37499612 | MS.gene97055:CDS |
| AAGTACTGATGATGAATTTA+GGG | 0.305836 | 2.2:+37499750 | MS.gene97055:CDS |
| GGACACATGCCAAAGATTCT+TGG | 0.310114 | 2.2:-37499211 | None:intergenic |
| AAAGACCTTCCAAGAATCTT+TGG | 0.314251 | 2.2:+37499202 | MS.gene97055:CDS |
| AAGGTAGCTGTATGAAGGTT+AGG | 0.324878 | 2.2:-37499127 | None:intergenic |
| GTCTTTATGTTGGATACCTT+TGG | 0.333066 | 2.2:-37499185 | None:intergenic |
| AATGGAATGAATGGATTCTA+AGG | 0.339360 | 2.2:-37499146 | None:intergenic |
| TATTCCTAAACTTAAGCATT+TGG | 0.349186 | 2.2:+37499480 | MS.gene97055:CDS |
| CTCTCCAAATGCTTAAGTTT+AGG | 0.351147 | 2.2:-37499484 | None:intergenic |
| TAGTTTGGAGAATAGGATAA+AGG | 0.362241 | 2.2:+37499669 | MS.gene97055:CDS |
| ATCCTTTGTCTTGAAGTAAT+GGG | 0.365238 | 2.2:+37499073 | MS.gene97055:CDS |
| AATCCTTTGTCTTGAAGTAA+TGG | 0.396956 | 2.2:+37499072 | MS.gene97055:CDS |
| GGAGACAAGAAAAGTGATAA+TGG | 0.414952 | 2.2:-37499164 | None:intergenic |
| ACACTAGAAGTAAGCAATCT+TGG | 0.430968 | 2.2:-37499343 | None:intergenic |
| AAAAGTGATAATGGAATGAA+TGG | 0.434056 | 2.2:-37499155 | None:intergenic |
| ATTAAGAGATTTAGAAGCAT+TGG | 0.444652 | 2.2:+37499411 | MS.gene97055:CDS |
| TTTAGGGAGTTGATAAAGAA+GGG | 0.454349 | 2.2:+37499766 | MS.gene97055:CDS |
| AGAGCTATGGTTCTAGCAGC+TGG | 0.458017 | 2.2:-37498902 | None:intergenic |
| AATAAGTGTTCTTTCAACAT+TGG | 0.460379 | 2.2:-37499461 | None:intergenic |
| GGTTAGACACATGGAGGTTG+TGG | 0.463307 | 2.2:+37499690 | MS.gene97055:CDS |
| TTCTTGGAAGGTCTTTATGT+TGG | 0.468294 | 2.2:-37499195 | None:intergenic |
| CACATGGAGGTTGTGGAAAG+TGG | 0.473398 | 2.2:+37499697 | MS.gene97055:CDS |
| ATACTAAAAGTCAACAATGC+AGG | 0.478012 | 2.2:-37499553 | None:intergenic |
| TTGGTTGAAGGAGGTGATGA+TGG | 0.480821 | 2.2:-37498962 | None:intergenic |
| AGGTAGCTGTATGAAGGTTA+GGG | 0.481780 | 2.2:-37499126 | None:intergenic |
| TAAAGAAGAGGCTAGGTGTA+TGG | 0.485234 | 2.2:+37499519 | MS.gene97055:CDS |
| CTTAAGCATTTGGAGAGTAT+GGG | 0.487893 | 2.2:+37499490 | MS.gene97055:CDS |
| ATTTAGGGAGTTGATAAAGA+AGG | 0.499226 | 2.2:+37499765 | MS.gene97055:CDS |
| TTGGGAGTTAAATTGGTTGA+AGG | 0.501320 | 2.2:-37498974 | None:intergenic |
| GGAGTTAAATTGGTTGAAGG+AGG | 0.504389 | 2.2:-37498971 | None:intergenic |
| AGAAGATGAGTGTAGAGCTA+TGG | 0.532807 | 2.2:-37498915 | None:intergenic |
| CTGTTAAGATAAAACAAAGC+TGG | 0.538212 | 2.2:-37499382 | None:intergenic |
| ATTGCTTACTTCTAGTGTGG+TGG | 0.553869 | 2.2:+37499348 | MS.gene97055:CDS |
| AAGATTGCTTACTTCTAGTG+TGG | 0.564951 | 2.2:+37499345 | MS.gene97055:CDS |
| TACTAAAAGTCAACAATGCA+GGG | 0.568581 | 2.2:-37499552 | None:intergenic |
| ATTCTAAGGTAGCTGTATGA+AGG | 0.573875 | 2.2:-37499132 | None:intergenic |
| TAGGGAGTTGATAAAGAAGG+GGG | 0.580226 | 2.2:+37499768 | MS.gene97055:CDS |
| GGAAATGAAGGGGAAATTGG+AGG | 0.580999 | 2.2:+37499615 | MS.gene97055:CDS |
| ACATGGAGGTTGTGGAAAGT+GGG | 0.587398 | 2.2:+37499698 | MS.gene97055:CDS |
| TCATGAGTAGGTGTGTAAAG+TGG | 0.589759 | 2.2:-37499022 | None:intergenic |
| AGGCTAGGTGTATGGTGTTG+AGG | 0.591790 | 2.2:+37499527 | MS.gene97055:CDS |
| GAGCTATGGTTCTAGCAGCT+GGG | 0.599605 | 2.2:-37498901 | None:intergenic |
| TATGGGATTTACTAAAGAAG+AGG | 0.599972 | 2.2:+37499507 | MS.gene97055:CDS |
| TGCCCATTACTTCAAGACAA+AGG | 0.602484 | 2.2:-37499075 | None:intergenic |
| TTAGGGAGTTGATAAAGAAG+GGG | 0.610325 | 2.2:+37499767 | MS.gene97055:CDS |
| GATTTACTAAAGAAGAGGCT+AGG | 0.635507 | 2.2:+37499512 | MS.gene97055:CDS |
| GAGTAGGTGTGTAAAGTGGA+TGG | 0.647606 | 2.2:-37499018 | None:intergenic |
| TAGGATAAAGGTTAGACACA+TGG | 0.648833 | 2.2:+37499681 | MS.gene97055:CDS |
| ACATGCCAAAGATTCTTGGA+AGG | 0.652767 | 2.2:-37499207 | None:intergenic |
| AAACTAAAAGAGAAATACTG+AGG | 0.668925 | 2.2:-37499652 | None:intergenic |
| GATAAAGGTTAGACACATGG+AGG | 0.698797 | 2.2:+37499684 | MS.gene97055:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATTTGAGTATTTTTCTG+TGG | + | chr2.2:37499594-37499613 | MS.gene97055:CDS | 20.0% |
| !!! | ACAGAAAAATACTCAAATTT+TGG | - | chr2.2:37499595-37499614 | None:intergenic | 20.0% |
| !!! | TTGTTTTATCTTAACAGATT+AGG | + | chr2.2:37499388-37499407 | MS.gene97055:CDS | 20.0% |
| ! | AAACTAAAAGAGAAATACTG+AGG | - | chr2.2:37499655-37499674 | None:intergenic | 25.0% |
| ! | AATAAGTGTTCTTTCAACAT+TGG | - | chr2.2:37499464-37499483 | None:intergenic | 25.0% |
| ! | GTATGAGAAAATCAAAAACT+GGG | - | chr2.2:37499273-37499292 | None:intergenic | 25.0% |
| ! | TATTCCTAAACTTAAGCATT+TGG | + | chr2.2:37499480-37499499 | MS.gene97055:CDS | 25.0% |
| ! | TGTATGAGAAAATCAAAAAC+TGG | - | chr2.2:37499274-37499293 | None:intergenic | 25.0% |
| !! | ATTAAGAGATTTAGAAGCAT+TGG | + | chr2.2:37499411-37499430 | MS.gene97055:CDS | 25.0% |
| !! | TATTTTTCTGTGGAAATGAA+GGG | + | chr2.2:37499604-37499623 | MS.gene97055:CDS | 25.0% |
| !! | TTATCAAGATTGTGTTCTTT+TGG | + | chr2.2:37499435-37499454 | MS.gene97055:CDS | 25.0% |
| !!! | AAAAGTGATAATGGAATGAA+TGG | - | chr2.2:37499158-37499177 | None:intergenic | 25.0% |
| !!! | AAAGTACTGATGATGAATTT+AGG | + | chr2.2:37499749-37499768 | MS.gene97055:CDS | 25.0% |
| !!! | AAGTACTGATGATGAATTTA+GGG | + | chr2.2:37499750-37499769 | MS.gene97055:CDS | 25.0% |
| !!! | TCAGTATTTCTCTTTTAGTT+TGG | + | chr2.2:37499654-37499673 | MS.gene97055:CDS | 25.0% |
| AATCCTTTGTCTTGAAGTAA+TGG | + | chr2.2:37499072-37499091 | MS.gene97055:CDS | 30.0% | |
| ATACTAAAAGTCAACAATGC+AGG | - | chr2.2:37499556-37499575 | None:intergenic | 30.0% | |
| ATCCTTTGTCTTGAAGTAAT+GGG | + | chr2.2:37499073-37499092 | MS.gene97055:CDS | 30.0% | |
| CTGTTAAGATAAAACAAAGC+TGG | - | chr2.2:37499385-37499404 | None:intergenic | 30.0% | |
| TACTAAAAGTCAACAATGCA+GGG | - | chr2.2:37499555-37499574 | None:intergenic | 30.0% | |
| TAGTTTGGAGAATAGGATAA+AGG | + | chr2.2:37499669-37499688 | MS.gene97055:CDS | 30.0% | |
| TATGGGATTTACTAAAGAAG+AGG | + | chr2.2:37499507-37499526 | MS.gene97055:CDS | 30.0% | |
| ! | AATGGAATGAATGGATTCTA+AGG | - | chr2.2:37499149-37499168 | None:intergenic | 30.0% |
| ! | ATTTTTCTGTGGAAATGAAG+GGG | + | chr2.2:37499605-37499624 | MS.gene97055:CDS | 30.0% |
| ! | CTTTTAAGCATCAATGACAA+AGG | - | chr2.2:37499733-37499752 | None:intergenic | 30.0% |
| ! | GTATTTTTCTGTGGAAATGA+AGG | + | chr2.2:37499603-37499622 | MS.gene97055:CDS | 30.0% |
| !! | ATTTAGGGAGTTGATAAAGA+AGG | + | chr2.2:37499765-37499784 | MS.gene97055:CDS | 30.0% |
| !! | TTTAGGGAGTTGATAAAGAA+GGG | + | chr2.2:37499766-37499785 | MS.gene97055:CDS | 30.0% |
| !!! | ACTTTTCTAAAGCTATGATC+AGG | - | chr2.2:37499310-37499329 | None:intergenic | 30.0% |
| !!! | TCTCTTTTAGTTTGGAGAAT+AGG | + | chr2.2:37499662-37499681 | MS.gene97055:CDS | 30.0% |
| AAAGACCTTCCAAGAATCTT+TGG | + | chr2.2:37499202-37499221 | MS.gene97055:CDS | 35.0% | |
| AAGATTGCTTACTTCTAGTG+TGG | + | chr2.2:37499345-37499364 | MS.gene97055:CDS | 35.0% | |
| ACACTAGAAGTAAGCAATCT+TGG | - | chr2.2:37499346-37499365 | None:intergenic | 35.0% | |
| ACTTAAGCATTTGGAGAGTA+TGG | + | chr2.2:37499489-37499508 | MS.gene97055:CDS | 35.0% | |
| ATGCTTGATGTGAGAATCTT+AGG | - | chr2.2:37499235-37499254 | None:intergenic | 35.0% | |
| CTCTCCAAATGCTTAAGTTT+AGG | - | chr2.2:37499487-37499506 | None:intergenic | 35.0% | |
| GATTTACTAAAGAAGAGGCT+AGG | + | chr2.2:37499512-37499531 | MS.gene97055:CDS | 35.0% | |
| GGAGACAAGAAAAGTGATAA+TGG | - | chr2.2:37499167-37499186 | None:intergenic | 35.0% | |
| GTCTTTATGTTGGATACCTT+TGG | - | chr2.2:37499188-37499207 | None:intergenic | 35.0% | |
| TAGGATAAAGGTTAGACACA+TGG | + | chr2.2:37499681-37499700 | MS.gene97055:CDS | 35.0% | |
| TTCTTGGAAGGTCTTTATGT+TGG | - | chr2.2:37499198-37499217 | None:intergenic | 35.0% | |
| TTGGGAGTTAAATTGGTTGA+AGG | - | chr2.2:37498977-37498996 | None:intergenic | 35.0% | |
| ! | AGTGAGAGTTTTTCATGAGT+AGG | - | chr2.2:37499037-37499056 | None:intergenic | 35.0% |
| ! | CTTAAGCATTTGGAGAGTAT+GGG | + | chr2.2:37499490-37499509 | MS.gene97055:CDS | 35.0% |
| ! | TTTGGGTTTGGGAGTTAAAT+TGG | - | chr2.2:37498984-37499003 | None:intergenic | 35.0% |
| !! | ATCACTTTTCTTGTCTCCAA+AGG | + | chr2.2:37499169-37499188 | MS.gene97055:CDS | 35.0% |
| !! | ATTCTAAGGTAGCTGTATGA+AGG | - | chr2.2:37499135-37499154 | None:intergenic | 35.0% |
| !! | TTAGGGAGTTGATAAAGAAG+GGG | + | chr2.2:37499767-37499786 | MS.gene97055:CDS | 35.0% |
| !!! | ATGGTTTTGAAGGAGACTTT+TGG | - | chr2.2:37499002-37499021 | None:intergenic | 35.0% |
| !!! | TGGTTTTGAAGGAGACTTTT+GGG | - | chr2.2:37499001-37499020 | None:intergenic | 35.0% |
| !!! | TGTAAAGTGGATGGTTTTGA+AGG | - | chr2.2:37499012-37499031 | None:intergenic | 35.0% |
| AAGGTAGCTGTATGAAGGTT+AGG | - | chr2.2:37499130-37499149 | None:intergenic | 40.0% | |
| ACATGCCAAAGATTCTTGGA+AGG | - | chr2.2:37499210-37499229 | None:intergenic | 40.0% | |
| AGAAGATGAGTGTAGAGCTA+TGG | - | chr2.2:37498918-37498937 | None:intergenic | 40.0% | |
| AGGTAGCTGTATGAAGGTTA+GGG | - | chr2.2:37499129-37499148 | None:intergenic | 40.0% | |
| ATTGCTTACTTCTAGTGTGG+TGG | + | chr2.2:37499348-37499367 | MS.gene97055:CDS | 40.0% | |
| GATAAAGGTTAGACACATGG+AGG | + | chr2.2:37499684-37499703 | MS.gene97055:CDS | 40.0% | |
| GGAGTTAAATTGGTTGAAGG+AGG | - | chr2.2:37498974-37498993 | None:intergenic | 40.0% | |
| TAAAGAAGAGGCTAGGTGTA+TGG | + | chr2.2:37499519-37499538 | MS.gene97055:CDS | 40.0% | |
| TCATGAGTAGGTGTGTAAAG+TGG | - | chr2.2:37499025-37499044 | None:intergenic | 40.0% | |
| TGCCCATTACTTCAAGACAA+AGG | - | chr2.2:37499078-37499097 | None:intergenic | 40.0% | |
| TGTGGAAATGAAGGGGAAAT+TGG | + | chr2.2:37499612-37499631 | MS.gene97055:CDS | 40.0% | |
| !! | GAAGTAATGGGCATTGATTC+AGG | + | chr2.2:37499085-37499104 | MS.gene97055:CDS | 40.0% |
| !! | TAGGGAGTTGATAAAGAAGG+GGG | + | chr2.2:37499768-37499787 | MS.gene97055:CDS | 40.0% |
| !!! | TGAAGGAGACTTTTGGGTTT+GGG | - | chr2.2:37498995-37499014 | None:intergenic | 40.0% |
| !!! | TTGAAGGAGACTTTTGGGTT+TGG | - | chr2.2:37498996-37499015 | None:intergenic | 40.0% |
| ACATGGAGGTTGTGGAAAGT+GGG | + | chr2.2:37499698-37499717 | MS.gene97055:CDS | 45.0% | |
| GAGTAGGTGTGTAAAGTGGA+TGG | - | chr2.2:37499021-37499040 | None:intergenic | 45.0% | |
| GGAAATGAAGGGGAAATTGG+AGG | + | chr2.2:37499615-37499634 | MS.gene97055:CDS | 45.0% | |
| GGACACATGCCAAAGATTCT+TGG | - | chr2.2:37499214-37499233 | None:intergenic | 45.0% | |
| TTGGTTGAAGGAGGTGATGA+TGG | - | chr2.2:37498965-37498984 | None:intergenic | 45.0% | |
| CACATGGAGGTTGTGGAAAG+TGG | + | chr2.2:37499697-37499716 | MS.gene97055:CDS | 50.0% | |
| GGTTAGACACATGGAGGTTG+TGG | + | chr2.2:37499690-37499709 | MS.gene97055:CDS | 50.0% | |
| ! | AGGCTAGGTGTATGGTGTTG+AGG | + | chr2.2:37499527-37499546 | MS.gene97055:CDS | 50.0% |
| !! | AGAGCTATGGTTCTAGCAGC+TGG | - | chr2.2:37498905-37498924 | None:intergenic | 50.0% |
| !! | GAGCTATGGTTCTAGCAGCT+GGG | - | chr2.2:37498904-37498923 | None:intergenic | 50.0% |
| !! | GAGTTGATAAAGAAGGGGGC+TGG | + | chr2.2:37499772-37499791 | MS.gene97055:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 37498896 | 37499798 | 37498896 | ID=MS.gene97055 |
| chr2.2 | mRNA | 37498896 | 37499798 | 37498896 | ID=MS.gene97055.t1;Parent=MS.gene97055 |
| chr2.2 | exon | 37498896 | 37499798 | 37498896 | ID=MS.gene97055.t1.exon1;Parent=MS.gene97055.t1 |
| chr2.2 | CDS | 37498896 | 37499798 | 37498896 | ID=cds.MS.gene97055.t1;Parent=MS.gene97055.t1 |
| Gene Sequence |
| Protein sequence |
