AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000176.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000176.01.T01 MTR_1g021995 98.077 52 1 0 1 52 210 261 2.85e-29 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000176.01.T01 AT1G47750 67.308 52 17 0 1 52 197 248 3.36e-19 77.0

Find 9 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGCTTTGGATGATATTT+TGG 0.183924 1:-2402107 MsG0180000176.01.T01:CDS
AGATTTGGCTGATGGATTAA+TGG 0.238206 1:-2402125 MsG0180000176.01.T01:CDS
TCCAGCAGATGACAACAAAA+TGG 0.295903 1:+2402060 None:intergenic
GCTTTCAATTGTTCAAGATT+TGG 0.305698 1:-2402140 MsG0180000176.01.T01:CDS
TGATCAGTACTCATAAGAAT+TGG 0.312137 1:-2402024 MsG0180000176.01.T01:CDS
GGCTGATGGATTAATGGCTT+TGG 0.368741 1:-2402119 MsG0180000176.01.T01:CDS
GATCAGTACTCATAAGAATT+GGG 0.413893 1:-2402023 MsG0180000176.01.T01:CDS
ATTGTTCAAGATTTGGCTGA+TGG 0.452124 1:-2402133 MsG0180000176.01.T01:CDS
CCAGCAGATGACAACAAAAT+GGG 0.495057 1:+2402061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATCAGTACTCATAAGAATT+GGG - Chr1:2402137-2402156 MsG0180000176.01.T01:CDS 30.0%
GCTTTCAATTGTTCAAGATT+TGG - Chr1:2402020-2402039 MsG0180000176.01.T01:CDS 30.0%
TGATCAGTACTCATAAGAAT+TGG - Chr1:2402136-2402155 MsG0180000176.01.T01:CDS 30.0%
!! CTTTGGATGATATTTTGGAT+GGG - Chr1:2402058-2402077 MsG0180000176.01.T01:CDS 30.0%
!!! AATGGCTTTGGATGATATTT+TGG - Chr1:2402053-2402072 MsG0180000176.01.T01:CDS 30.0%
ATTGTTCAAGATTTGGCTGA+TGG - Chr1:2402027-2402046 MsG0180000176.01.T01:CDS 35.0%
!! AGATTTGGCTGATGGATTAA+TGG - Chr1:2402035-2402054 MsG0180000176.01.T01:CDS 35.0%
!! GATGATATTTTGGATGGGAA+GGG - Chr1:2402063-2402082 MsG0180000176.01.T01:CDS 35.0%
!! GCTTTGGATGATATTTTGGA+TGG - Chr1:2402057-2402076 MsG0180000176.01.T01:CDS 35.0%
CCAGCAGATGACAACAAAAT+GGG + Chr1:2402102-2402121 None:intergenic 40.0%
TCCAGCAGATGACAACAAAA+TGG + Chr1:2402103-2402122 None:intergenic 40.0%
!! GGATGATATTTTGGATGGGA+AGG - Chr1:2402062-2402081 MsG0180000176.01.T01:CDS 40.0%
!! CCCATTTTGTTGTCATCTGC+TGG - Chr1:2402099-2402118 MsG0180000176.01.T01:CDS 45.0%
!! GGCTGATGGATTAATGGCTT+TGG - Chr1:2402041-2402060 MsG0180000176.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 2402012 2402170 2402012 ID=MsG0180000176.01;Name=MsG0180000176.01
Chr1 mRNA 2402012 2402170 2402012 ID=MsG0180000176.01.T01;Parent=MsG0180000176.01;Name=MsG0180000176.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|52
Chr1 exon 2402012 2402170 2402012 ID=MsG0180000176.01.T01:exon:6653;Parent=MsG0180000176.01.T01
Chr1 CDS 2402012 2402170 2402012 ID=MsG0180000176.01.T01:cds;Parent=MsG0180000176.01.T01
Gene Sequence

>MsG0180000176.01.T01

ATGAAGAAGCTTTCAATTGTTCAAGATTTGGCTGATGGATTAATGGCTTTGGATGATATTTTGGATGGGAAGGGATTGTTTTCGAAGCCCATTTTGTTGTCATCTGCTGGACTTTTGTCTGCATTGATCAGTACTCATAAGAATTGGGTTTCTTGCTGA

Protein sequence

>MsG0180000176.01.T01

MKKLSIVQDLADGLMALDDILDGKGLFSKPILLSSAGLLSALISTHKNWVSC*