AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021492.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021492.01.T01 MTR_4g074450 96.429 140 5 0 1 140 1 140 1.88e-97 277
MsG0480021492.01.T01 MTR_4g108000 96.429 140 5 0 1 140 254 393 6.11e-95 280
MsG0480021492.01.T01 MTR_4g108000 96.429 140 5 0 1 140 269 408 1.22e-94 279
MsG0480021492.01.T01 MTR_4g074450 92.254 142 9 1 1 142 1 140 9.45e-94 268
MsG0480021492.01.T01 MTR_4g108000 91.724 145 8 2 1 141 269 413 1.62e-90 270
MsG0480021492.01.T01 MTR_1g012600 44.048 84 45 1 49 132 47 128 1.32e-17 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021492.01.T01 AT3G07860 63.380 142 47 2 3 141 2 141 1.58e-51 161
MsG0480021492.01.T01 AT3G07860 63.380 142 47 2 3 141 2 141 1.58e-51 161
MsG0480021492.01.T01 AT3G07860 65.414 133 43 1 3 132 2 134 3.62e-50 157
MsG0480021492.01.T01 AT5G25340 40.964 83 47 1 50 132 19 99 4.71e-17 74.7
MsG0480021492.01.T01 AT4G32270 33.333 84 54 1 49 132 37 118 5.86e-15 69.7

Find 35 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGATGTGCTTCAAAATT+TGG 0.144653 4:-58569383 MsG0480021492.01.T01:CDS
AGCAAATTTGCGTTTGAATT+TGG 0.188144 4:-58569882 MsG0480021492.01.T01:five_prime_UTR
GTAATTAACTTTCTTCTTGA+TGG 0.243951 4:+58569612 None:intergenic
ATCTTCAAGCGTAGGATTCT+TGG 0.255078 4:+58569756 None:intergenic
AACTCTCATTGGACTTGAAT+TGG 0.259182 4:-58569728 MsG0480021492.01.T01:CDS
TTGATTGCAGGAGGAGTGTT+TGG 0.266654 4:-58569450 MsG0480021492.01.T01:intron
TTACATGGAACAATCTAGTA+TGG 0.294231 4:-58569593 MsG0480021492.01.T01:CDS
GAAGGTGTTGAAAGCATTAT+TGG 0.319812 4:-58569803 MsG0480021492.01.T01:CDS
TGGGGCATCGTCACATTTCT+TGG 0.329427 4:-58569573 MsG0480021492.01.T01:intron
TTTCTGAAATAAAGTGAGTT+TGG 0.336025 4:-58569911 MsG0480021492.01.T01:five_prime_UTR
TATCTATTCTATGTCATTGC+TGG 0.345615 4:-58569328 MsG0480021492.01.T01:CDS
TATGAATACGGCAACGCTTA+AGG 0.362851 4:-58569647 MsG0480021492.01.T01:CDS
TGATGTGATTCTTATGAATA+CGG 0.363774 4:-58569659 MsG0480021492.01.T01:CDS
GAAGCACATCATCATCGTTA+AGG 0.405288 4:+58569392 None:intergenic
ACTCTCATTGGACTTGAATT+GGG 0.418691 4:-58569727 MsG0480021492.01.T01:CDS
TTGGGTACATCTGCAAGTAT+TGG 0.422385 4:+58569775 None:intergenic
GATGATGTGCTTCAAAATTT+GGG 0.438672 4:-58569382 MsG0480021492.01.T01:CDS
TGATTGCAGGAGGAGTGTTT+GGG 0.443491 4:-58569449 MsG0480021492.01.T01:intron
TCTTCAAGCGTAGGATTCTT+GGG 0.457805 4:+58569757 None:intergenic
TTGGTTATTGTGCGATGAGA+TGG 0.459607 4:-58569863 MsG0480021492.01.T01:exon
GGGCGTTCGAAATAATTCTC+AGG 0.483534 4:-58569362 MsG0480021492.01.T01:CDS
CTGAAATAAAGTGAGTTTGG+TGG 0.485053 4:-58569908 MsG0480021492.01.T01:five_prime_UTR
ATACAAGAGCGGTTTGAAGA+AGG 0.490103 4:-58569827 MsG0480021492.01.T01:CDS
TGGTTATTGTGCGATGAGAT+GGG 0.507966 4:-58569862 MsG0480021492.01.T01:exon
GAAGATGTGGAAACTCTCAT+TGG 0.517568 4:-58569739 MsG0480021492.01.T01:CDS
GAATCCTACGCTTGAAGATG+TGG 0.526900 4:-58569752 MsG0480021492.01.T01:CDS
CAAGAAGAAAGTTAATTACA+TGG 0.538196 4:-58569608 MsG0480021492.01.T01:CDS
GTTTCCACATCTTCAAGCGT+AGG 0.543211 4:+58569748 None:intergenic
TACATGGAACAATCTAGTAT+GGG 0.546371 4:-58569592 MsG0480021492.01.T01:CDS
GGTGTTGAAAGCATTATTGG+AGG 0.583098 4:-58569800 MsG0480021492.01.T01:CDS
TTGTGCGATGAGATGGGAGA+AGG 0.646862 4:-58569856 MsG0480021492.01.T01:exon
ACATGGAACAATCTAGTATG+GGG 0.649258 4:-58569591 MsG0480021492.01.T01:CDS
GAGCGGTTTGAAGAAGGCGA+AGG 0.652966 4:-58569821 MsG0480021492.01.T01:CDS
CGATGAGATGGGAGAAGGTG+CGG 0.664098 4:-58569851 MsG0480021492.01.T01:exon
GAAGGTGCGGAATACAAGAG+CGG 0.727904 4:-58569838 MsG0480021492.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCAAACATTGAAAAATGAT+TGG - Chr4:58569738-58569757 MsG0480021492.01.T01:CDS 25.0%
! CAAGAAGAAAGTTAATTACA+TGG - Chr4:58569637-58569656 MsG0480021492.01.T01:CDS 25.0%
! GTAATTAACTTTCTTCTTGA+TGG + Chr4:58569636-58569655 None:intergenic 25.0%
! TGATGTGATTCTTATGAATA+CGG - Chr4:58569586-58569605 MsG0480021492.01.T01:CDS 25.0%
!! TTTCAATGTTTGCTAATCAA+TGG + Chr4:58569732-58569751 None:intergenic 25.0%
!!! TTTCTGAAATAAAGTGAGTT+TGG - Chr4:58569334-58569353 MsG0480021492.01.T01:CDS 25.0%
AGCAAATTTGCGTTTGAATT+TGG - Chr4:58569363-58569382 MsG0480021492.01.T01:CDS 30.0%
GATGATGTGCTTCAAAATTT+GGG - Chr4:58569863-58569882 MsG0480021492.01.T01:exon 30.0%
TACATGGAACAATCTAGTAT+GGG - Chr4:58569653-58569672 MsG0480021492.01.T01:CDS 30.0%
TATCTATTCTATGTCATTGC+TGG - Chr4:58569917-58569936 MsG0480021492.01.T01:five_prime_UTR 30.0%
TTACATGGAACAATCTAGTA+TGG - Chr4:58569652-58569671 MsG0480021492.01.T01:CDS 30.0%
! TGATGATGTGCTTCAAAATT+TGG - Chr4:58569862-58569881 MsG0480021492.01.T01:exon 30.0%
!!! ATCTGATTGATTTTGATTGC+AGG - Chr4:58569783-58569802 MsG0480021492.01.T01:CDS 30.0%
!!! GCGAATTTCTGTTTTGAAAT+TGG - Chr4:58569550-58569569 MsG0480021492.01.T01:intron 30.0%
AACTCTCATTGGACTTGAAT+TGG - Chr4:58569517-58569536 MsG0480021492.01.T01:intron 35.0%
ACATGGAACAATCTAGTATG+GGG - Chr4:58569654-58569673 MsG0480021492.01.T01:CDS 35.0%
ACTCTCATTGGACTTGAATT+GGG - Chr4:58569518-58569537 MsG0480021492.01.T01:intron 35.0%
CTGAAATAAAGTGAGTTTGG+TGG - Chr4:58569337-58569356 MsG0480021492.01.T01:CDS 35.0%
!! GAAGGTGTTGAAAGCATTAT+TGG - Chr4:58569442-58569461 MsG0480021492.01.T01:CDS 35.0%
!!! TGATTGATTTTGATTGCAGG+AGG - Chr4:58569786-58569805 MsG0480021492.01.T01:CDS 35.0%
ATACAAGAGCGGTTTGAAGA+AGG - Chr4:58569418-58569437 MsG0480021492.01.T01:CDS 40.0%
ATCTTCAAGCGTAGGATTCT+TGG + Chr4:58569492-58569511 None:intergenic 40.0%
CATTGCTGGAATTGCGAAAT+AGG - Chr4:58569931-58569950 MsG0480021492.01.T01:five_prime_UTR 40.0%
GAAGATGTGGAAACTCTCAT+TGG - Chr4:58569506-58569525 MsG0480021492.01.T01:intron 40.0%
GAAGCACATCATCATCGTTA+AGG + Chr4:58569856-58569875 None:intergenic 40.0%
TATGAATACGGCAACGCTTA+AGG - Chr4:58569598-58569617 MsG0480021492.01.T01:CDS 40.0%
TCTTCAAGCGTAGGATTCTT+GGG + Chr4:58569491-58569510 None:intergenic 40.0%
TGGTTATTGTGCGATGAGAT+GGG - Chr4:58569383-58569402 MsG0480021492.01.T01:CDS 40.0%
TTGGGTACATCTGCAAGTAT+TGG + Chr4:58569473-58569492 None:intergenic 40.0%
TTGGTTATTGTGCGATGAGA+TGG - Chr4:58569382-58569401 MsG0480021492.01.T01:CDS 40.0%
!! GGTGTTGAAAGCATTATTGG+AGG - Chr4:58569445-58569464 MsG0480021492.01.T01:intron 40.0%
GAATCCTACGCTTGAAGATG+TGG - Chr4:58569493-58569512 MsG0480021492.01.T01:intron 45.0%
GGGCGTTCGAAATAATTCTC+AGG - Chr4:58569883-58569902 MsG0480021492.01.T01:five_prime_UTR 45.0%
GTTTCCACATCTTCAAGCGT+AGG + Chr4:58569500-58569519 None:intergenic 45.0%
!! TGATTGCAGGAGGAGTGTTT+GGG - Chr4:58569796-58569815 MsG0480021492.01.T01:CDS 45.0%
!! TTGATTGCAGGAGGAGTGTT+TGG - Chr4:58569795-58569814 MsG0480021492.01.T01:CDS 45.0%
TTGTGCGATGAGATGGGAGA+AGG - Chr4:58569389-58569408 MsG0480021492.01.T01:CDS 50.0%
! GAAGGTGCGGAATACAAGAG+CGG - Chr4:58569407-58569426 MsG0480021492.01.T01:CDS 50.0%
!! TGGGGCATCGTCACATTTCT+TGG - Chr4:58569672-58569691 MsG0480021492.01.T01:CDS 50.0%
CGATGAGATGGGAGAAGGTG+CGG - Chr4:58569394-58569413 MsG0480021492.01.T01:CDS 55.0%
GAGCGGTTTGAAGAAGGCGA+AGG - Chr4:58569424-58569443 MsG0480021492.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 58569315 58569952 58569315 ID=MsG0480021492.01;Name=MsG0480021492.01
Chr4 mRNA 58569315 58569952 58569315 ID=MsG0480021492.01.T01;Parent=MsG0480021492.01;Name=MsG0480021492.01.T01;_AED=0.38;_eAED=0.39;_QI=86|1|0.5|1|1|1|2|0|146
Chr4 exon 58569315 58569462 58569315 ID=MsG0480021492.01.T01:exon:17345;Parent=MsG0480021492.01.T01
Chr4 exon 58569574 58569952 58569574 ID=MsG0480021492.01.T01:exon:17344;Parent=MsG0480021492.01.T01
Chr4 five_prime_UTR 58569867 58569952 58569867 ID=MsG0480021492.01.T01:five_prime_utr;Parent=MsG0480021492.01.T01
Chr4 CDS 58569574 58569866 58569574 ID=MsG0480021492.01.T01:cds;Parent=MsG0480021492.01.T01
Chr4 CDS 58569315 58569462 58569315 ID=MsG0480021492.01.T01:cds;Parent=MsG0480021492.01.T01
Gene Sequence

>MsG0480021492.01.T01

ATGGGAGAAGGTGCGGAATACAAGAGCGGTTTGAAGAAGGCGAAGGTGTTGAAAGCATTATTGGAGGATCCAATACTTGCAGATGTACCCAAGAATCCTACGCTTGAAGATGTGGAAACTCTCATTGGACTTGAATTGGGTAGTGCAATGCGAATTTCTGTTTTGAAATTGGATTCAACTTCGTTTGATGTGATTCTTATGAATACGGCAACGCTTAAGGATTTGAAACTTGCCATCAAGAAGAAAGTTAATTACATGGAACAATCTAGTATGGGGCATCGTCACATTTCTTGGAGGAGTGTTTGGGCTAATTATTGTTTGTCATTCGATAACAACAAGCTCCTTAACGATGATGATGTGCTTCAAAATTTGGGCGTTCGAAATAATTCTCAGGTTTCTTCAACTTATCTATTCTATGTCATTGCTGGAATTGCGAAATAG

Protein sequence

>MsG0480021492.01.T01

MGEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRISVLKLDSTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLFYVIAGIAK*