AlfalfaGEDB | MsG0780037123.01

M. sativa cultivar ZhongmuNo.1 / MsG0780037123.01

KEGG: -
GO: GO:0003677 GO:0005634
PFAM: PF03110

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037123.01.T01	PNX74892.1	60.116	173	54	4	1	161	1	170	4.03E-56	184

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037123.01.T01	A0A2K3L8N2	60.116	173	54	4	1	161	1	170	1.93e-56	184

TFs/TRs:

Gene ID	Type	Classification
MsG0780037123.01.T01	TF	SBP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048461.01	MsG0780037123.01	0.833392	5.475888e-56	6.297562e-53
MsG0080048462.01	MsG0780037123.01	0.826762	2.270198e-54	2.148760e-51
MsG0080048856.01	MsG0780037123.01	0.829108	6.191258e-55	6.275234e-52
MsG0180000091.01	MsG0780037123.01	0.821171	4.652159e-53	3.756921e-50
MsG0180000295.01	MsG0780037123.01	0.802894	4.517969e-49	2.238983e-46
MsG0180000334.01	MsG0780037123.01	0.840424	8.769957e-58	1.248659e-54
MsG0180000335.01	MsG0780037123.01	0.809349	1.977074e-50	1.158111e-47
MsG0180000470.01	MsG0780037123.01	0.815475	9.072337e-52	6.261756e-49
MsG0180000555.01	MsG0780037123.01	0.823961	1.044932e-53	9.131459e-51
MsG0180000702.01	MsG0780037123.01	0.821284	4.382826e-53	3.550251e-50
MsG0180000703.01	MsG0780037123.01	0.812757	3.611247e-51	2.315384e-48
MsG0180000741.01	MsG0780037123.01	0.800901	1.159387e-48	5.462556e-46
MsG0180000836.01	MsG0780037123.01	0.856863	2.413142e-62	5.840268e-59
MsG0180000914.01	MsG0780037123.01	0.803427	3.503670e-49	1.760194e-46
MsG0180001173.01	MsG0780037123.01	0.816409	5.613371e-52	3.974272e-49
MsG0180001566.01	MsG0780037123.01	0.808340	3.250390e-50	1.853770e-47
MsG0180001662.01	MsG0780037123.01	0.814966	1.177148e-51	8.011676e-49
MsG0180002377.01	MsG0780037123.01	0.805143	1.538892e-49	8.080270e-47
MsG0180002952.01	MsG0780037123.01	0.828482	8.772285e-55	8.730644e-52
MsG0180002992.01	MsG0780037123.01	0.814096	1.833060e-51	1.218601e-48
MsG0180003455.01	MsG0780037123.01	0.842199	2.992182e-58	4.502373e-55
MsG0180003807.01	MsG0780037123.01	0.814896	1.219537e-51	8.284354e-49
MsG0180003810.01	MsG0780037123.01	0.830674	2.571652e-55	2.728340e-52
MsG0180003885.01	MsG0780037123.01	0.808710	2.709514e-50	1.560177e-47
MsG0180003922.01	MsG0780037123.01	0.803980	2.690967e-49	1.371306e-46
MsG0180003932.01	MsG0780037123.01	0.824460	7.974794e-54	7.067746e-51
MsG0180004331.01	MsG0780037123.01	0.808546	2.936731e-50	1.683906e-47
MsG0180004486.01	MsG0780037123.01	0.802130	6.490462e-49	3.154504e-46
MsG0180004493.01	MsG0780037123.01	0.809716	1.648922e-50	9.753360e-48
MsG0180004500.01	MsG0780037123.01	0.820214	7.719090e-53	6.069938e-50
MsG0180004725.01	MsG0780037123.01	0.815569	8.644709e-52	5.981607e-49
MsG0180004802.01	MsG0780037123.01	0.805122	1.554789e-49	8.159288e-47
MsG0180005049.01	MsG0780037123.01	0.800826	1.201295e-48	5.648763e-46
MsG0180005185.01	MsG0780037123.01	0.805944	1.045140e-49	5.601847e-47
MsG0180005304.01	MsG0780037123.01	0.803598	3.229982e-49	1.629881e-46
MsG0180005443.01	MsG0780037123.01	0.838620	2.580506e-57	3.475766e-54
MsG0180005445.01	MsG0780037123.01	0.872390	3.299178e-67	1.378142e-63
MsG0180005451.01	MsG0780037123.01	0.838414	2.917528e-57	3.904846e-54
MsG0180005708.01	MsG0780037123.01	0.802423	5.650984e-49	2.767171e-46
MsG0180005786.01	MsG0780037123.01	0.809506	1.829735e-50	1.076218e-47
MsG0180006025.01	MsG0780037123.01	0.803372	3.597878e-49	1.804875e-46
MsG0180006134.01	MsG0780037123.01	0.822016	2.968482e-53	2.454679e-50
MsG0180006155.01	MsG0780037123.01	0.802808	4.705784e-49	2.327091e-46
MsG0280010598.01	MsG0780037123.01	0.839899	1.201946e-57	1.683689e-54
MsG0280010599.01	MsG0780037123.01	0.857177	1.949987e-62	4.770687e-59
MsG0280010816.01	MsG0780037123.01	0.819083	1.399211e-52	1.066163e-49
MsG0280011333.01	MsG0780037123.01	0.814187	1.750447e-51	1.166572e-48
MsG0280011334.01	MsG0780037123.01	0.824903	6.271850e-54	5.630732e-51
MsG0280011444.01	MsG0780037123.01	0.811172	7.998112e-51	4.916096e-48
MsG0380012057.01	MsG0780037123.01	0.821096	4.841682e-53	3.901903e-50
MsG0380012067.01	MsG0780037123.01	0.816056	6.732427e-52	4.721100e-49
MsG0380012103.01	MsG0780037123.01	0.824546	7.611412e-54	6.763258e-51
MsG0380012160.01	MsG0780037123.01	0.804175	2.450531e-49	1.254983e-46
MsG0380013176.01	MsG0780037123.01	0.811127	8.176641e-51	5.020067e-48
MsG0380013475.01	MsG0780037123.01	0.814100	1.829721e-51	1.216491e-48
MsG0380014537.01	MsG0780037123.01	0.800337	1.510907e-48	7.017392e-46
MsG0380014576.01	MsG0780037123.01	0.812782	3.565640e-51	2.287582e-48
MsG0380014701.01	MsG0780037123.01	0.824618	7.320827e-54	6.519096e-51
MsG0680030880.01	MsG0780037123.01	0.800887	1.167301e-48	5.497824e-46
MsG0680031214.01	MsG0780037123.01	0.810281	1.245992e-50	7.479361e-48
MsG0680031505.01	MsG0780037123.01	0.801131	1.040644e-48	4.931619e-46
MsG0680031506.01	MsG0780037123.01	0.801424	9.063858e-49	4.326969e-46
MsG0680033465.01	MsG0780037123.01	0.830901	2.261683e-55	2.415673e-52
MsG0680034757.01	MsG0780037123.01	0.809241	2.085123e-50	1.217892e-47
MsG0680034913.01	MsG0780037123.01	0.800403	1.464782e-48	6.814546e-46
MsG0680035005.01	MsG0780037123.01	0.801450	8.951223e-49	4.276151e-46
MsG0680035034.01	MsG0780037123.01	0.802753	4.829745e-49	2.385004e-46
MsG0680035040.01	MsG0780037123.01	0.814196	1.742589e-51	1.161587e-48
MsG0480018465.01	MsG0780037123.01	0.816724	4.771869e-52	3.407771e-49
MsG0480019148.01	MsG0780037123.01	0.806906	6.551772e-50	3.599122e-47
MsG0480019970.01	MsG0780037123.01	0.812497	4.115319e-51	2.620712e-48
MsG0480020739.01	MsG0780037123.01	0.811931	5.469664e-51	3.430091e-48
MsG0480020780.01	MsG0780037123.01	0.805988	1.023254e-49	5.490744e-47
MsG0480021056.01	MsG0780037123.01	0.835328	1.789343e-56	2.181146e-53
MsG0480021409.01	MsG0780037123.01	0.807867	4.097391e-50	2.307729e-47
MsG0480021489.01	MsG0780037123.01	0.826166	3.149541e-54	2.930851e-51
MsG0480021492.01	MsG0780037123.01	0.813878	2.047590e-51	1.353251e-48
MsG0480021534.01	MsG0780037123.01	0.813576	2.386294e-51	1.564230e-48
MsG0480021870.01	MsG0780037123.01	0.860732	1.682651e-63	4.646197e-60
MsG0480021900.01	MsG0780037123.01	0.810729	9.973742e-51	6.059057e-48
MsG0480021918.01	MsG0780037123.01	0.819935	8.940967e-53	6.976778e-50
MsG0480022318.01	MsG0780037123.01	0.802187	6.318532e-49	3.075305e-46
MsG0480023172.01	MsG0780037123.01	0.820323	7.287132e-53	5.747371e-50
MsG0480023354.01	MsG0780037123.01	0.842808	2.061780e-58	3.162243e-55
MsG0480023451.01	MsG0780037123.01	0.814476	1.511328e-51	1.015164e-48
MsG0480023463.01	MsG0780037123.01	0.820417	6.937180e-53	5.486104e-50
MsG0480023488.01	MsG0780037123.01	0.809841	1.550192e-50	9.199513e-48
MsG0480023670.01	MsG0780037123.01	0.854897	9.064290e-62	2.055154e-58
MsG0480023718.01	MsG0780037123.01	0.821663	3.581296e-53	2.932079e-50
MsG0580024157.01	MsG0780037123.01	0.836310	1.008828e-56	1.266639e-53
MsG0580024197.01	MsG0780037123.01	0.804010	2.652588e-49	1.352735e-46
MsG0580024306.01	MsG0780037123.01	0.801667	8.080522e-49	3.881312e-46
MsG0580024690.01	MsG0780037123.01	0.812940	3.292381e-51	2.121127e-48
MsG0580024781.01	MsG0780037123.01	0.813638	2.312837e-51	1.518581e-48
MsG0580024960.01	MsG0780037123.01	0.809968	1.455488e-50	8.666277e-48
MsG0580025008.01	MsG0780037123.01	0.803016	4.262806e-49	2.118959e-46
MsG0580025022.01	MsG0780037123.01	0.807245	5.553924e-50	3.078052e-47
MsG0580025206.01	MsG0780037123.01	0.844830	5.929893e-59	9.692289e-56
MsG0580025332.01	MsG0780037123.01	0.829917	3.936097e-55	4.085274e-52
MsG0580025532.01	MsG0780037123.01	0.812075	5.087509e-51	3.203137e-48
MsG0580025555.01	MsG0780037123.01	0.821303	4.337328e-53	3.515419e-50
MsG0580025661.01	MsG0780037123.01	0.808338	3.252401e-50	1.854842e-47
MsG0580025900.01	MsG0780037123.01	0.822259	2.606500e-53	2.170564e-50
MsG0380014948.01	MsG0780037123.01	0.846854	1.672110e-59	2.916814e-56
MsG0380014949.01	MsG0780037123.01	0.840706	7.397679e-58	1.062555e-54
MsG0380015220.01	MsG0780037123.01	0.803161	3.978619e-49	1.985195e-46
MsG0380015332.01	MsG0780037123.01	0.836350	9.852998e-57	1.238534e-53
MsG0380015440.01	MsG0780037123.01	0.814258	1.688577e-51	1.127505e-48
MsG0380015555.01	MsG0780037123.01	0.804913	1.719559e-49	8.976096e-47
MsG0380016005.01	MsG0780037123.01	0.820915	5.329000e-53	4.272992e-50
MsG0380016236.01	MsG0780037123.01	0.806716	7.185728e-50	3.928030e-47
MsG0380016505.01	MsG0780037123.01	0.813551	2.416934e-51	1.583259e-48
MsG0380016694.01	MsG0780037123.01	0.815197	1.046040e-51	7.163962e-49
MsG0380016923.01	MsG0780037123.01	0.836397	9.582433e-57	1.206098e-53
MsG0380017375.01	MsG0780037123.01	0.814855	1.245691e-51	8.452754e-49
MsG0480018183.01	MsG0780037123.01	0.822861	1.888133e-53	1.599549e-50
MsG0480018251.01	MsG0780037123.01	0.804971	1.672268e-49	8.741782e-47
MsG0480018313.01	MsG0780037123.01	0.805574	1.250267e-49	6.637490e-47
MsG0580027891.01	MsG0780037123.01	0.845353	4.282910e-59	7.118089e-56
MsG0580028517.01	MsG0780037123.01	0.831359	1.745490e-55	1.889752e-52
MsG0580028592.01	MsG0780037123.01	0.800701	1.273946e-48	5.971294e-46
MsG0580029009.01	MsG0780037123.01	0.800208	1.605038e-48	7.429951e-46
MsG0580029231.01	MsG0780037123.01	0.804872	1.753234e-49	9.142591e-47
MsG0580029236.01	MsG0780037123.01	0.813932	1.992596e-51	1.318744e-48
MsG0580029349.01	MsG0780037123.01	0.815298	9.931011e-52	6.820576e-49
MsG0580029622.01	MsG0780037123.01	0.827919	1.199195e-54	1.174015e-51
MsG0580029651.01	MsG0780037123.01	0.800231	1.587844e-48	7.354744e-46
MsG0580029919.01	MsG0780037123.01	0.800937	1.140316e-48	5.377352e-46
MsG0580029921.01	MsG0780037123.01	0.806536	7.843170e-50	4.267746e-47
MsG0580029926.01	MsG0780037123.01	0.813745	2.191370e-51	1.442960e-48
MsG0680030321.01	MsG0780037123.01	0.801209	1.003243e-48	4.763462e-46
MsG0680030447.01	MsG0780037123.01	0.835665	1.469807e-56	1.809592e-53
MsG0680030674.01	MsG0780037123.01	0.800930	1.143596e-48	5.392038e-46
MsG0680030677.01	MsG0780037123.01	0.835697	1.442800e-56	1.778223e-53
MsG0280006601.01	MsG0780037123.01	0.822197	2.694398e-53	2.239652e-50
MsG0280006692.01	MsG0780037123.01	0.820707	5.949423e-53	4.742563e-50
MsG0280006930.01	MsG0780037123.01	0.813714	2.225927e-51	1.464588e-48
MsG0280007527.01	MsG0780037123.01	0.815248	1.018953e-51	6.988130e-49
MsG0280007531.01	MsG0780037123.01	0.819909	9.066763e-53	7.069729e-50
MsG0280007710.01	MsG0780037123.01	0.808017	3.807350e-50	2.152923e-47
MsG0280007756.01	MsG0780037123.01	0.803189	3.925793e-49	1.960245e-46
MsG0280008100.01	MsG0780037123.01	0.803141	4.016659e-49	2.003092e-46
MsG0280008113.01	MsG0780037123.01	0.810638	1.043433e-50	6.323231e-48
MsG0280008363.01	MsG0780037123.01	0.853914	1.743235e-61	3.826504e-58
MsG0280008378.01	MsG0780037123.01	0.806772	6.994651e-50	3.829039e-47
MsG0280008615.01	MsG0780037123.01	0.825647	4.182185e-54	3.834810e-51
MsG0280008696.01	MsG0780037123.01	0.808928	2.433876e-50	1.409565e-47
MsG0280008738.01	MsG0780037123.01	0.803745	3.010377e-49	1.524769e-46
MsG0280009417.01	MsG0780037123.01	0.823471	1.360901e-53	1.172824e-50
MsG0280009421.01	MsG0780037123.01	0.803989	2.678540e-49	1.365313e-46
MsG0280009544.01	MsG0780037123.01	0.813202	2.883507e-51	1.870883e-48
MsG0280009748.01	MsG0780037123.01	0.805032	1.623306e-49	8.498883e-47
MsG0280010109.01	MsG0780037123.01	0.834238	3.364686e-56	3.968931e-53
MsG0280010184.01	MsG0780037123.01	0.810850	9.389901e-51	5.722947e-48
MsG0280010233.01	MsG0780037123.01	0.806445	8.196339e-50	4.449593e-47
MsG0280010350.01	MsG0780037123.01	0.830208	3.341882e-55	3.497776e-52
MsG0280010366.01	MsG0780037123.01	0.809283	2.042405e-50	1.194247e-47
MsG0280010411.01	MsG0780037123.01	0.811954	5.405746e-51	3.392308e-48
MsG0280010491.01	MsG0780037123.01	0.814488	1.501540e-51	1.008943e-48
MsG0280010517.01	MsG0780037123.01	0.803917	2.772621e-49	1.410665e-46
MsG0780036430.01	MsG0780037123.01	0.820978	5.154085e-53	4.140274e-50
MsG0780036602.01	MsG0780037123.01	0.807850	4.132470e-50	2.326470e-47
MsG0780036773.01	MsG0780037123.01	0.849375	3.366451e-60	6.369305e-57
MsG0780037054.01	MsG0780037123.01	0.830632	2.632129e-55	2.789003e-52
MsG0780037123.01	MsG0780037141.01	0.810577	1.075427e-50	6.506672e-48
MsG0780037123.01	MsG0780037144.01	0.823890	1.085495e-53	9.467148e-51
MsG0780037123.01	MsG0780037146.01	0.829347	5.415224e-55	5.528327e-52
MsG0780037123.01	MsG0780037630.01	0.810823	9.515319e-51	5.795096e-48
MsG0780037123.01	MsG0780038051.01	0.828982	6.639848e-55	6.704429e-52
MsG0780037123.01	MsG0780038439.01	0.817371	3.414200e-52	2.481878e-49
MsG0780037123.01	MsG0780038443.01	0.808208	3.466948e-50	1.970469e-47
MsG0780037123.01	MsG0780038602.01	0.823747	1.172937e-53	1.018912e-50
MsG0780037123.01	MsG0780038603.01	0.823796	1.142266e-53	9.935996e-51
MsG0780037123.01	MsG0780038907.01	0.845191	4.736195e-59	7.830204e-56
MsG0780037123.01	MsG0780039155.01	0.802025	6.823855e-49	3.307607e-46
MsG0780037123.01	MsG0780039170.01	0.828672	7.891635e-55	7.897438e-52
MsG0780037123.01	MsG0780039257.01	0.803808	2.921034e-49	1.481910e-46
MsG0780037123.01	MsG0780039443.01	0.850141	2.056639e-60	3.989442e-57
MsG0780037123.01	MsG0780039655.01	0.839024	2.029256e-57	2.766919e-54
MsG0780037123.01	MsG0780039866.01	0.803141	4.017067e-49	2.003271e-46
MsG0780037123.01	MsG0780040176.01	0.806714	7.194536e-50	3.932595e-47
MsG0780037123.01	MsG0780040591.01	0.817183	3.763214e-52	2.721452e-49
MsG0780037123.01	MsG0780040669.01	0.808540	2.945836e-50	1.688836e-47
MsG0780037123.01	MsG0780041127.01	0.801405	9.145276e-49	4.363751e-46
MsG0780037123.01	MsG0780041258.01	0.802995	4.305848e-49	2.139223e-46
MsG0780037123.01	MsG0780041305.01	0.829053	6.384184e-55	6.459739e-52
MsG0780037123.01	MsG0780041387.01	0.820501	6.634686e-53	5.258709e-50
MsG0780037123.01	MsG0780041654.01	0.811968	5.367618e-51	3.369668e-48
MsG0780037123.01	MsG0880042324.01	0.837775	4.260337e-57	5.592457e-54
MsG0780037123.01	MsG0880042348.01	0.813937	1.987906e-51	1.315799e-48
MsG0780037123.01	MsG0880042518.01	0.806415	8.317518e-50	4.511761e-47
MsG0780037123.01	MsG0880042547.01	0.801092	1.059920e-48	5.018156e-46
MsG0780037123.01	MsG0880042939.01	0.810717	1.003482e-50	6.094228e-48
MsG0780037123.01	MsG0880043479.01	0.813627	2.325622e-51	1.526556e-48
MsG0780037123.01	MsG0880043480.01	0.820091	8.235576e-53	6.454852e-50
MsG0780037123.01	MsG0880043521.01	0.804159	2.468844e-49	1.263867e-46
MsG0780037123.01	MsG0880043522.01	0.809536	1.802592e-50	1.061129e-47
MsG0780037123.01	MsG0880044067.01	0.805093	1.576524e-49	8.267033e-47
MsG0780037123.01	MsG0880044565.01	0.812920	3.325006e-51	2.141040e-48
MsG0780037123.01	MsG0880045111.01	0.803143	4.012250e-49	2.000983e-46
MsG0780037123.01	MsG0880045294.01	0.832548	8.878468e-56	9.956429e-53
MsG0780037123.01	MsG0880045329.01	0.809607	1.740797e-50	1.026727e-47
MsG0780037123.01	MsG0880045372.01	0.821658	3.590914e-53	2.939518e-50
MsG0780037123.01	MsG0880045639.01	0.832068	1.167297e-55	1.290537e-52
MsG0780037123.01	MsG0880046027.01	0.839892	1.207599e-57	1.691134e-54
MsG0780037123.01	MsG0880047055.01	-0.804494	2.102365e-49	1.085524e-46
MsG0780037123.01	MsG0880047473.01	0.805832	1.103407e-49	5.897156e-47
MsG0780037123.01	MsG0880047696.01	0.801976	6.981426e-49	3.379685e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037123.01.T01	MTR_7g028740	64.000	175	49	4	1	161	1	175	2.79e-59	188
MsG0780037123.01.T01	MTR_3g099080	32.161	199	90	7	1	159	1	194	1.83e-18	81.3
MsG0780037123.01.T01	MTR_8g096780	64.444	45	15	1	115	159	151	194	1.61e-12	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 30 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAAATCAGTTCATGATTC+AGG	0.275617	7:+20196394	MsG0780037123.01.T01:CDS
AACCAACACAACAGGAGTTT+TGG	0.287936	7:-20196583	None:intergenic
GGATTTGAGGCTTGGTGATC+AGG	0.330157	7:+20196357	MsG0780037123.01.T01:CDS
ATGAGGTTAAGAGGAGTTGC+AGG	0.333125	7:+20197063	MsG0780037123.01.T01:CDS
AAAAGAGCGCGTTTGCAAAA+TGG	0.355601	7:+20196466	MsG0780037123.01.T01:CDS
GAGAGTATCATAAGCGTCAT+AGG	0.369267	7:+20196544	MsG0780037123.01.T01:CDS
GGGATTGGAAAGAGTTTACT+TGG	0.383641	7:+20196290	MsG0780037123.01.T01:CDS
TGATCAGGCTTCTGATTCAT+TGG	0.384148	7:+20196372	MsG0780037123.01.T01:CDS
GAGTTTACTTGGGATCCATC+TGG	0.428398	7:+20196301	MsG0780037123.01.T01:CDS
AAAACTCCTGTTGTGTTGGT+TGG	0.434108	7:+20196585	MsG0780037123.01.T01:CDS
GATTCAATGGATTTGAGGCT+TGG	0.443840	7:+20196349	MsG0780037123.01.T01:CDS
AAGGAGATTCAATGGATTTG+AGG	0.446707	7:+20196344	MsG0780037123.01.T01:CDS
GAATATGATTGCTCGGTTGA+TGG	0.451779	7:+20196495	MsG0780037123.01.T01:CDS
GCGAGTTTGATGAGGTTAAG+AGG	0.452089	7:+20197054	MsG0780037123.01.T01:CDS
TACTTGGGATCCATCTGGAT+TGG	0.477477	7:+20196306	MsG0780037123.01.T01:CDS
TTGGGATCCATCTGGATTGG+AGG	0.483016	7:+20196309	MsG0780037123.01.T01:CDS
AGTTGCAGGAAACGTCTTGA+TGG	0.488371	7:+20197077	MsG0780037123.01.T01:CDS
TTACCTCCAACCAACACAAC+AGG	0.509843	7:-20196591	None:intergenic
ACTCCTGTTGTGTTGGTTGG+AGG	0.530507	7:+20196588	MsG0780037123.01.T01:CDS
ATGAAAAGAGACTGTGGCTG+AGG	0.533136	7:-20197122	None:intergenic
TTCAACTCCTCCAATCCAGA+TGG	0.535266	7:-20196316	None:intergenic
TTCCAAAACTCCTGTTGTGT+TGG	0.540226	7:+20196581	MsG0780037123.01.T01:CDS
GATCGCAGAATATGATTGCT+CGG	0.570479	7:+20196488	MsG0780037123.01.T01:CDS
ATCAGTTCATGATTCAGGAA+AGG	0.585059	7:+20196399	MsG0780037123.01.T01:CDS
ATGGACATAATAAGCGCAGA+AGG	0.585971	7:+20197096	MsG0780037123.01.T01:CDS
AGAGTATCATAAGCGTCATA+GGG	0.590533	7:+20196545	MsG0780037123.01.T01:CDS
ATTCACTTGCGAGTTTGATG+AGG	0.612796	7:+20197046	MsG0780037123.01.T01:CDS
GGATTGGAAAGAGTTTACTT+GGG	0.616910	7:+20196291	MsG0780037123.01.T01:CDS
AAGTTCATGTACAATTACAA+AGG	0.619439	7:+20197155	MsG0780037123.01.T01:CDS
GCAGCCATGAAAAGAGACTG+TGG	0.673886	7:-20197128	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTATCTGAATATTCTAAAAA+TGG	+	Chr7:20196927-20196946	MsG0780037123.01.T01:intron	20.0%
!	AAGTTCATGTACAATTACAA+AGG	+	Chr7:20197155-20197174	MsG0780037123.01.T01:CDS	25.0%
!	ATATTCAGATAGCTTCTATA+TGG	-	Chr7:20196919-20196938	None:intergenic	25.0%
!	TGAATTGTAGTTGTTTGTTT+CGG	+	Chr7:20196649-20196668	MsG0780037123.01.T01:intron	25.0%
	GAAAAATGAAGGAGATTCAA+TGG	+	Chr7:20196336-20196355	MsG0780037123.01.T01:CDS	30.0%
	GAATTGTAGTTGTTTGTTTC+GGG	+	Chr7:20196650-20196669	MsG0780037123.01.T01:intron	30.0%
!!	TTGAGGTTGTTTTGTAACAT+GGG	+	Chr7:20196999-20197018	MsG0780037123.01.T01:intron	30.0%
!!	TTTGAGGTTGTTTTGTAACA+TGG	+	Chr7:20196998-20197017	MsG0780037123.01.T01:intron	30.0%
	ACCTCAAAACATGATGTGTT+TGG	-	Chr7:20196986-20197005	None:intergenic	35.0%
	ATCAGTTCATGATTCAGGAA+AGG	+	Chr7:20196399-20196418	MsG0780037123.01.T01:CDS	35.0%
	GAGAAATCAGTTCATGATTC+AGG	+	Chr7:20196394-20196413	MsG0780037123.01.T01:CDS	35.0%
	GGATTGGAAAGAGTTTACTT+GGG	+	Chr7:20196291-20196310	MsG0780037123.01.T01:CDS	35.0%
	TTGGAGGAGTTGAAAAATGA+AGG	+	Chr7:20196325-20196344	MsG0780037123.01.T01:CDS	35.0%
!	AAGGAGATTCAATGGATTTG+AGG	+	Chr7:20196344-20196363	MsG0780037123.01.T01:CDS	35.0%
!	AGAGTATCATAAGCGTCATA+GGG	+	Chr7:20196545-20196564	MsG0780037123.01.T01:CDS	35.0%
!	TCCAAACACATCATGTTTTG+AGG	+	Chr7:20196982-20197001	MsG0780037123.01.T01:intron	35.0%
	ATGGACATAATAAGCGCAGA+AGG	+	Chr7:20197096-20197115	MsG0780037123.01.T01:CDS	40.0%
	ATTCACTTGCGAGTTTGATG+AGG	+	Chr7:20197046-20197065	MsG0780037123.01.T01:CDS	40.0%
	GAATATGATTGCTCGGTTGA+TGG	+	Chr7:20196495-20196514	MsG0780037123.01.T01:CDS	40.0%
	GATCGCAGAATATGATTGCT+CGG	+	Chr7:20196488-20196507	MsG0780037123.01.T01:CDS	40.0%
	GGGATTGGAAAGAGTTTACT+TGG	+	Chr7:20196290-20196309	MsG0780037123.01.T01:CDS	40.0%
	GGGTGATGTCAATTTGATGT+AGG	+	Chr7:20197019-20197038	MsG0780037123.01.T01:intron	40.0%
	TCATATACCTGCTGCATTGT+TGG	-	Chr7:20196632-20196651	None:intergenic	40.0%
	TGATCAGGCTTCTGATTCAT+TGG	+	Chr7:20196372-20196391	MsG0780037123.01.T01:CDS	40.0%
	TTCCAAAACTCCTGTTGTGT+TGG	+	Chr7:20196581-20196600	MsG0780037123.01.T01:CDS	40.0%
!	AAAACTCCTGTTGTGTTGGT+TGG	+	Chr7:20196585-20196604	MsG0780037123.01.T01:CDS	40.0%
!	AAAAGAGCGCGTTTGCAAAA+TGG	+	Chr7:20196466-20196485	MsG0780037123.01.T01:CDS	40.0%
!	AACCAACACAACAGGAGTTT+TGG	-	Chr7:20196586-20196605	None:intergenic	40.0%
!	GATTCAATGGATTTGAGGCT+TGG	+	Chr7:20196349-20196368	MsG0780037123.01.T01:CDS	40.0%
!	GCTCTTTTCAACGATCTAGT+AGG	-	Chr7:20196454-20196473	None:intergenic	40.0%
!!	GAGAGTATCATAAGCGTCAT+AGG	+	Chr7:20196544-20196563	MsG0780037123.01.T01:CDS	40.0%
	AGTTGCAGGAAACGTCTTGA+TGG	+	Chr7:20197077-20197096	MsG0780037123.01.T01:CDS	45.0%
	ATGAAAAGAGACTGTGGCTG+AGG	-	Chr7:20197125-20197144	None:intergenic	45.0%
	ATGAGGTTAAGAGGAGTTGC+AGG	+	Chr7:20197063-20197082	MsG0780037123.01.T01:CDS	45.0%
	GAGTTTACTTGGGATCCATC+TGG	+	Chr7:20196301-20196320	MsG0780037123.01.T01:CDS	45.0%
	GCGAGTTTGATGAGGTTAAG+AGG	+	Chr7:20197054-20197073	MsG0780037123.01.T01:CDS	45.0%
	TACTTGGGATCCATCTGGAT+TGG	+	Chr7:20196306-20196325	MsG0780037123.01.T01:CDS	45.0%
	TTACCTCCAACCAACACAAC+AGG	-	Chr7:20196594-20196613	None:intergenic	45.0%
	TTCAACTCCTCCAATCCAGA+TGG	-	Chr7:20196319-20196338	None:intergenic	45.0%
!	TCAGCCACAGTCTCTTTTCA+TGG	+	Chr7:20197124-20197143	MsG0780037123.01.T01:CDS	45.0%
	GCAGCCATGAAAAGAGACTG+TGG	-	Chr7:20197131-20197150	None:intergenic	50.0%
!	TTGGGATCCATCTGGATTGG+AGG	+	Chr7:20196309-20196328	MsG0780037123.01.T01:CDS	50.0%
!!	ACTCCTGTTGTGTTGGTTGG+AGG	+	Chr7:20196588-20196607	MsG0780037123.01.T01:CDS	50.0%
!!	GGATTTGAGGCTTGGTGATC+AGG	+	Chr7:20196357-20196376	MsG0780037123.01.T01:CDS	50.0%
	GGTTCTGCCAACAATGCAGC+AGG	+	Chr7:20196622-20196641	MsG0780037123.01.T01:intron	55.0%
	TGGTTGGAGGTAAGCAGCAG+AGG	+	Chr7:20196601-20196620	MsG0780037123.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	20196283	20197211	20196283	ID=MsG0780037123.01;Name=MsG0780037123.01
Chr7	mRNA	20196283	20197211	20196283	ID=MsG0780037123.01.T01;Parent=MsG0780037123.01;Name=MsG0780037123.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|0\|0\|1\|1\|1\|2\|0\|165
Chr7	exon	20196283	20196609	20196283	ID=MsG0780037123.01.T01:exon:4938;Parent=MsG0780037123.01.T01
Chr7	exon	20197041	20197211	20197041	ID=MsG0780037123.01.T01:exon:4939;Parent=MsG0780037123.01.T01
Chr7	CDS	20196283	20196609	20196283	ID=MsG0780037123.01.T01:cds;Parent=MsG0780037123.01.T01
Chr7	CDS	20197041	20197211	20197041	ID=MsG0780037123.01.T01:cds;Parent=MsG0780037123.01.T01

Gene Sequence

>MsG0780037123.01.T01

ATGGATTGGGATTGGAAAGAGTTTACTTGGGATCCATCTGGATTGGAGGAGTTGAAAAATGAAGGAGATTCAATGGATTTGAGGCTTGGTGATCAGGCTTCTGATTCATTGGAGAAATCAGTTCATGATTCAGGAAAGGAAGATTCAAAAGCTGTGTCGTCGTCGTCACCTACTAGATCGTTGAAAAGAGCGCGTTTGCAAAATGGATCGCAGAATATGATTGCTCGGTTGATGGATGTAACTCTGATCTTCGTGATTGTAGAGAGTATCATAAGCGTCATAGGGTGTGCGAAAAGCATTCCAAAACTCCTGTTGTGTTGGTTGGAGGTTTCATTCACTTGCGAGTTTGATGAGGTTAAGAGGAGTTGCAGGAAACGTCTTGATGGACATAATAAGCGCAGAAGGAAACCTCAGCCACAGTCTCTTTTCATGGCTGCTGAGAAGTTCATGTACAATTACAAAGGTTTGTGTCTTTTGCATCTCATGATTCTAAGATAG

Protein sequence

>MsG0780037123.01.T01

MDWDWKEFTWDPSGLEELKNEGDSMDLRLGDQASDSLEKSVHDSGKEDSKAVSSSSPTRSLKRARLQNGSQNMIARLMDVTLIFVIVESIISVIGCAKSIPKLLLCWLEVSFTCEFDEVKRSCRKRLDGHNKRRRKPQPQSLFMAAEKFMYNYKGLCLLHLMILR*