AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047160.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047160.01.T01 MTR_4g122060 89.655 58 6 0 4 61 290 347 5.76e-28 108
MsG0880047160.01.T01 MTR_4g122060 97.959 49 1 0 76 124 177 225 3.61e-27 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047160.01.T01 AT3G17040 75.510 49 12 0 76 124 193 241 2.39e-21 89.0
MsG0880047160.01.T01 AT3G17040 72.881 59 16 0 4 62 306 364 3.88e-21 88.2
MsG0880047160.01.T01 AT3G17040 75.510 49 12 0 76 124 159 207 2.64e-21 88.6
MsG0880047160.01.T01 AT3G17040 70.492 61 18 0 2 62 270 330 4.66e-21 88.2
MsG0880047160.01.T01 AT3G17040 75.510 49 12 0 76 124 159 207 2.79e-21 89.0
MsG0880047160.01.T01 AT3G17040 72.881 59 16 0 4 62 272 330 6.30e-21 88.2
MsG0880047160.01.T01 AT3G17040 75.510 49 12 0 76 124 193 241 2.87e-21 89.0
MsG0880047160.01.T01 AT3G17040 72.881 59 16 0 4 62 306 364 5.66e-21 88.2

Find 33 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGTTGTTGTCTTGTTCA+TGG 0.303084 8:-82821020 MsG0880047160.01.T01:CDS
CATGGAAGTGTTTAAGCTAT+TGG 0.327680 8:-82821002 MsG0880047160.01.T01:CDS
GGCTCGATATTTATTCAATC+AGG 0.377634 8:-82821730 MsG0880047160.01.T01:CDS
CTAATAGGCTTGGGCTCAAA+TGG 0.378024 8:-82821571 MsG0880047160.01.T01:intron
GTGAATGAGAAGCTACATAA+TGG 0.378900 8:-82821048 MsG0880047160.01.T01:intron
GGCTCAAATGGAGGTGGAAC+AGG 0.382782 8:-82821559 MsG0880047160.01.T01:CDS
ACATACAGTCTACCATCTTC+CGG 0.444757 8:+82820979 None:intergenic
GATTTATGAGAAGGGTTGCC+AGG 0.448335 8:-82820905 MsG0880047160.01.T01:CDS
AGGGTGAAAATGCATTCATT+TGG 0.448950 8:-82820876 MsG0880047160.01.T01:CDS
TGAATGAGAAGCTACATAAT+GGG 0.450775 8:-82821047 MsG0880047160.01.T01:intron
ATCCTAAAAGCTGTGCTAGT+TGG 0.452145 8:-82821695 MsG0880047160.01.T01:CDS
GAAGGGTTGCCAGGCTACTC+AGG 0.453699 8:-82820896 MsG0880047160.01.T01:CDS
AAGCAATGCAAGTGTGTGTT+TGG 0.456923 8:+82821779 None:intergenic
ATGTTATGTATTATTTGTGA+TGG 0.464798 8:-82820826 MsG0880047160.01.T01:CDS
GGTAGACTGTATGTTGCATT+GGG 0.480510 8:-82820970 MsG0880047160.01.T01:CDS
TGAAAATGCATTCATTTGGC+AGG 0.484233 8:-82820872 MsG0880047160.01.T01:CDS
ACTTGCATTGCTTGAAGCTA+AGG 0.501379 8:-82821769 MsG0880047160.01.T01:CDS
AGCTAGAGAGATTTATGAGA+AGG 0.510163 8:-82820914 MsG0880047160.01.T01:CDS
TTAAGCTATTGGCCGGAAGA+TGG 0.530889 8:-82820991 MsG0880047160.01.T01:CDS
GAAGTGTTTAAGCTATTGGC+CGG 0.545721 8:-82820998 MsG0880047160.01.T01:CDS
TGTTATGTATTATTTGTGAT+GGG 0.551484 8:-82820825 MsG0880047160.01.T01:CDS
TACCGCTAGGAAATTGTTCG+AGG 0.552980 8:-82821526 MsG0880047160.01.T01:intron
GGCTTGGGCTCAAATGGAGG+TGG 0.554574 8:-82821565 MsG0880047160.01.T01:CDS
TGGTAGACTGTATGTTGCAT+TGG 0.555872 8:-82820971 MsG0880047160.01.T01:CDS
GCTAGAGAGATTTATGAGAA+GGG 0.568643 8:-82820913 MsG0880047160.01.T01:CDS
TAAGGCGAATAGATATCAGC+AGG 0.578086 8:-82821751 MsG0880047160.01.T01:CDS
AAGGGTTGCCAGGCTACTCA+GGG 0.619016 8:-82820895 MsG0880047160.01.T01:CDS
AGGAAAACTATCGTACCGCT+AGG 0.622711 8:-82821539 MsG0880047160.01.T01:CDS
ATAGGCTTGGGCTCAAATGG+AGG 0.638738 8:-82821568 MsG0880047160.01.T01:intron
ATTATTTGTGATGGGTCATG+AGG 0.643404 8:-82820817 None:intergenic
TTATTTGTGATGGGTCATGA+GGG 0.652180 8:-82820816 None:intergenic
AACCTCGAACAATTTCCTAG+CGG 0.752535 8:+82821524 None:intergenic
GTAGACTGTATGTTGCATTG+GGG 0.778600 8:-82820969 MsG0880047160.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATATTTATTTTATCTAAT+AGG - Chr8:82821025-82821044 MsG0880047160.01.T01:CDS 10.0%
!! AAATTATTTCATTATGACTT+AGG - Chr8:82821173-82821192 MsG0880047160.01.T01:intron 15.0%
!! AAATCAAACTATTAGGTTAT+TGG - Chr8:82820975-82820994 MsG0880047160.01.T01:CDS 20.0%
!! AATAACCTAATAGTTTGATT+TGG + Chr8:82820976-82820995 None:intergenic 20.0%
!! ATGTTATGTATTATTTGTGA+TGG - Chr8:82821785-82821804 MsG0880047160.01.T01:CDS 20.0%
!! CACAATAAACCATATAAAAA+AGG + Chr8:82821546-82821565 None:intergenic 20.0%
!! TGTTATGTATTATTTGTGAT+GGG - Chr8:82821786-82821805 MsG0880047160.01.T01:CDS 20.0%
!!! TTATTTTATCTAATAGGCTT+GGG - Chr8:82821031-82821050 MsG0880047160.01.T01:CDS 20.0%
!!! TTTATTTTATCTAATAGGCT+TGG - Chr8:82821030-82821049 MsG0880047160.01.T01:CDS 20.0%
! AACTTGAATAATGATAGTAG+TGG + Chr8:82821518-82821537 None:intergenic 25.0%
! TAAACCATATAAAAAAGGAC+AGG + Chr8:82821541-82821560 None:intergenic 25.0%
! TGTTTCCAAATCAAACTATT+AGG - Chr8:82820968-82820987 MsG0880047160.01.T01:CDS 25.0%
AGGGAAGAAAACTTAAAGAT+AGG - Chr8:82821322-82821341 MsG0880047160.01.T01:intron 30.0%
TGAATAATGATAGTAGTGGT+AGG + Chr8:82821514-82821533 None:intergenic 30.0%
TGAATGAGAAGCTACATAAT+GGG - Chr8:82821564-82821583 MsG0880047160.01.T01:CDS 30.0%
!!! AGTTCCTGTCCTTTTTTATA+TGG - Chr8:82821534-82821553 MsG0880047160.01.T01:CDS 30.0%
!!! GCTTTTAGGATTATACTTTG+TGG + Chr8:82820907-82820926 None:intergenic 30.0%
AGCTAGAGAGATTTATGAGA+AGG - Chr8:82821697-82821716 MsG0880047160.01.T01:CDS 35.0%
AGGGTGAAAATGCATTCATT+TGG - Chr8:82821735-82821754 MsG0880047160.01.T01:CDS 35.0%
ATGAAGCAAGGAAATCAGAA+AGG + Chr8:82821143-82821162 None:intergenic 35.0%
CTATTAATGCAAGAGACTGA+AGG + Chr8:82821378-82821397 None:intergenic 35.0%
GCTAGAGAGATTTATGAGAA+GGG - Chr8:82821698-82821717 MsG0880047160.01.T01:CDS 35.0%
GGCTCGATATTTATTCAATC+AGG - Chr8:82820881-82820900 MsG0880047160.01.T01:CDS 35.0%
GTGAATGAGAAGCTACATAA+TGG - Chr8:82821563-82821582 MsG0880047160.01.T01:CDS 35.0%
TGAAAATGCATTCATTTGGC+AGG - Chr8:82821739-82821758 MsG0880047160.01.T01:CDS 35.0%
!! CATGGAAGTGTTTAAGCTAT+TGG - Chr8:82821609-82821628 MsG0880047160.01.T01:intron 35.0%
!! GTTTGTTGTTGTCTTGTTCA+TGG - Chr8:82821591-82821610 MsG0880047160.01.T01:intron 35.0%
AACCTCGAACAATTTCCTAG+CGG + Chr8:82821090-82821109 None:intergenic 40.0%
AAGCAATGCAAGTGTGTGTT+TGG + Chr8:82820835-82820854 None:intergenic 40.0%
AAGCCACTTACAAATCAAGC+AGG + Chr8:82821475-82821494 None:intergenic 40.0%
ACATACAGTCTACCATCTTC+CGG + Chr8:82821635-82821654 None:intergenic 40.0%
ACTTGCATTGCTTGAAGCTA+AGG - Chr8:82820842-82820861 MsG0880047160.01.T01:CDS 40.0%
ATCCTAAAAGCTGTGCTAGT+TGG - Chr8:82820916-82820935 MsG0880047160.01.T01:CDS 40.0%
GATTTGGAAACAATGTCGCT+CGG + Chr8:82820960-82820979 None:intergenic 40.0%
GGTAGACTGTATGTTGCATT+GGG - Chr8:82821641-82821660 MsG0880047160.01.T01:intron 40.0%
GTAGACTGTATGTTGCATTG+GGG - Chr8:82821642-82821661 MsG0880047160.01.T01:intron 40.0%
GTCCTAAAAATAGGTTCGCT+TGG - Chr8:82821256-82821275 MsG0880047160.01.T01:intron 40.0%
TAAGGCGAATAGATATCAGC+AGG - Chr8:82820860-82820879 MsG0880047160.01.T01:CDS 40.0%
TGCAGTCGAGTCCTAAAAAT+AGG - Chr8:82821247-82821266 MsG0880047160.01.T01:intron 40.0%
TGGTAGACTGTATGTTGCAT+TGG - Chr8:82821640-82821659 MsG0880047160.01.T01:intron 40.0%
TTGCTGAGTTCAATGAAGCA+AGG + Chr8:82821155-82821174 None:intergenic 40.0%
TTGTAAGTGGCTTACTCAGT+TGG - Chr8:82821482-82821501 MsG0880047160.01.T01:intron 40.0%
! GAAGTGTTTAAGCTATTGGC+CGG - Chr8:82821613-82821632 MsG0880047160.01.T01:intron 40.0%
!! AAATCAAGCAGGTTGATGCT+TGG + Chr8:82821464-82821483 None:intergenic 40.0%
!! AATCAAGCAGGTTGATGCTT+GGG + Chr8:82821463-82821482 None:intergenic 40.0%
!! CAACCTGCTTGATTTGTAAG+TGG - Chr8:82821469-82821488 MsG0880047160.01.T01:intron 40.0%
!! TGCCAAGCGAACCTATTTTT+AGG + Chr8:82821261-82821280 None:intergenic 40.0%
!!! GGCATTTTTGAAGCTAACCT+GGG - Chr8:82821288-82821307 MsG0880047160.01.T01:intron 40.0%
AACCTGGGCAAGATTGACAA+GGG - Chr8:82821303-82821322 MsG0880047160.01.T01:intron 45.0%
AGGAAAACTATCGTACCGCT+AGG - Chr8:82821072-82821091 MsG0880047160.01.T01:intron 45.0%
CTAATAGGCTTGGGCTCAAA+TGG - Chr8:82821040-82821059 MsG0880047160.01.T01:CDS 45.0%
GATTTATGAGAAGGGTTGCC+AGG - Chr8:82821706-82821725 MsG0880047160.01.T01:CDS 45.0%
TAACCTGGGCAAGATTGACA+AGG - Chr8:82821302-82821321 MsG0880047160.01.T01:intron 45.0%
TACCGCTAGGAAATTGTTCG+AGG - Chr8:82821085-82821104 MsG0880047160.01.T01:intron 45.0%
TTAAGCTATTGGCCGGAAGA+TGG - Chr8:82821620-82821639 MsG0880047160.01.T01:intron 45.0%
! AGCCAACTAGCACAGCTTTT+AGG + Chr8:82820921-82820940 None:intergenic 45.0%
! AGGCCATGCTCTAAATCCAA+GGG - Chr8:82821342-82821361 MsG0880047160.01.T01:intron 45.0%
! CATCCCTTGGATTTAGAGCA+TGG + Chr8:82821348-82821367 None:intergenic 45.0%
! TAGGCCATGCTCTAAATCCA+AGG - Chr8:82821341-82821360 MsG0880047160.01.T01:intron 45.0%
!! GTTCAAGAGAGCGTCTGAAT+TGG - Chr8:82821435-82821454 MsG0880047160.01.T01:intron 45.0%
!!! GGGCATTTTTGAAGCTAACC+TGG - Chr8:82821287-82821306 MsG0880047160.01.T01:intron 45.0%
ATAGGCTTGGGCTCAAATGG+AGG - Chr8:82821043-82821062 MsG0880047160.01.T01:intron 50.0%
ATAGGTTCGCTTGGCATGTC+TGG - Chr8:82821265-82821284 MsG0880047160.01.T01:intron 50.0%
TAGGTTCGCTTGGCATGTCT+GGG - Chr8:82821266-82821285 MsG0880047160.01.T01:intron 50.0%
TGAAGGAGAACAGCATCCCT+TGG + Chr8:82821361-82821380 None:intergenic 50.0%
TTCCCTTGTCAATCTTGCCC+AGG + Chr8:82821308-82821327 None:intergenic 50.0%
! CATTTTCACCCTGAGTAGCC+TGG + Chr8:82821727-82821746 None:intergenic 50.0%
AAGGGTTGCCAGGCTACTCA+GGG - Chr8:82821716-82821735 MsG0880047160.01.T01:CDS 55.0%
AGGTTCGCTTGGCATGTCTG+GGG - Chr8:82821267-82821286 MsG0880047160.01.T01:intron 55.0%
GGCTCAAATGGAGGTGGAAC+AGG - Chr8:82821052-82821071 MsG0880047160.01.T01:intron 55.0%
GAAGGGTTGCCAGGCTACTC+AGG - Chr8:82821715-82821734 MsG0880047160.01.T01:CDS 60.0%
GGCTTGGGCTCAAATGGAGG+TGG - Chr8:82821046-82821065 MsG0880047160.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 82820819 82821814 82820819 ID=MsG0880047160.01;Name=MsG0880047160.01
Chr8 mRNA 82820819 82821814 82820819 ID=MsG0880047160.01.T01;Parent=MsG0880047160.01;Name=MsG0880047160.01.T01;_AED=0.50;_eAED=0.66;_QI=0|0|0|1|0|0.33|3|0|141
Chr8 exon 82821692 82821814 82821692 ID=MsG0880047160.01.T01:exon:20532;Parent=MsG0880047160.01.T01
Chr8 exon 82821527 82821586 82821527 ID=MsG0880047160.01.T01:exon:20531;Parent=MsG0880047160.01.T01
Chr8 exon 82820819 82821061 82820819 ID=MsG0880047160.01.T01:exon:20530;Parent=MsG0880047160.01.T01
Chr8 CDS 82821692 82821814 82821692 ID=MsG0880047160.01.T01:cds;Parent=MsG0880047160.01.T01
Chr8 CDS 82821527 82821586 82821527 ID=MsG0880047160.01.T01:cds;Parent=MsG0880047160.01.T01
Chr8 CDS 82820819 82821061 82820819 ID=MsG0880047160.01.T01:cds;Parent=MsG0880047160.01.T01
Gene Sequence

>MsG0880047160.01.T01

ATGAGTACATATACCAAACACACACTTGCATTGCTTGAAGCTAAGGCGAATAGATATCAGCAGGCTCGATATTTATTCAATCAGGCCACAAAGTATAATCCTAAAAGCTGTGCTAGTTGGCTTGCTTGGGCTCAAATGGAGGTGGAACAGGAAAACTATCGTACCGCTAGGAAATTGTTCGAGAAGCTACATAATGGGTTTTGTTTGTTGTTGTCTTGTTCATGGAAGTGTTTAAGCTATTGGCCGGAAGATGGTAGACTGTATGTTGCATTGGGGAAAATTATGAGCAAGCAATCTAAAACGAGTCAAGCTAGAGAGATTTATGAGAAGGGTTGCCAGGCTACTCAGGGTGAAAATGCATTCATTTGGCAGGTTAGTGTTCAAGTCTGTTTTTGCATGTTATGTATTATTTGTGATGGGTCATGA

Protein sequence

>MsG0880047160.01.T01

MSTYTKHTLALLEAKANRYQQARYLFNQATKYNPKSCASWLAWAQMEVEQENYRTARKLFEKLHNGFCLLLSCSWKCLSYWPEDGRLYVALGKIMSKQSKTSQAREIYEKGCQATQGENAFIWQVSVQVCFCMLCIICDGS*