Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035403.01.T01 | XP_003598604.2 | 66.017 | 359 | 105 | 3 | 3 | 345 | 4 | 361 | 6.96E-162 | 470 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035403.01.T01 | Q8GYH5 | 37.377 | 305 | 176 | 5 | 8 | 302 | 4 | 303 | 6.46E-62 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035403.01.T01 | G7IYT6 | 66.017 | 359 | 105 | 3 | 3 | 345 | 4 | 361 | 3.33e-162 | 470 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035403.01.T01 | MTR_3g016210 | 66.017 | 359 | 105 | 3 | 3 | 345 | 4 | 361 | 8.43e-166 | 470 |
| MsG0680035403.01.T01 | MTR_8g039620 | 67.429 | 350 | 109 | 3 | 5 | 350 | 10 | 358 | 2.68e-165 | 469 |
| MsG0680035403.01.T01 | MTR_8g072510 | 66.571 | 350 | 114 | 3 | 1 | 348 | 1 | 349 | 6.14e-162 | 461 |
| MsG0680035403.01.T01 | MTR_8g073260 | 64.655 | 348 | 119 | 2 | 1 | 346 | 1 | 346 | 4.76e-159 | 453 |
| MsG0680035403.01.T01 | MTR_7g021090 | 65.429 | 350 | 112 | 5 | 1 | 348 | 1 | 343 | 9.39e-157 | 446 |
| MsG0680035403.01.T01 | MTR_1g059930 | 64.689 | 354 | 121 | 2 | 1 | 350 | 1 | 354 | 1.31e-150 | 431 |
| MsG0680035403.01.T01 | MTR_7g451420 | 67.192 | 317 | 102 | 2 | 1 | 316 | 1 | 316 | 1.18e-147 | 419 |
| MsG0680035403.01.T01 | MTR_8g068990 | 62.784 | 352 | 110 | 6 | 4 | 350 | 7 | 342 | 1.36e-147 | 424 |
| MsG0680035403.01.T01 | MTR_8g062410 | 63.610 | 349 | 116 | 5 | 3 | 348 | 4 | 344 | 1.83e-147 | 424 |
| MsG0680035403.01.T01 | MTR_8g069880 | 64.265 | 347 | 122 | 2 | 5 | 350 | 3 | 348 | 5.41e-147 | 423 |
| MsG0680035403.01.T01 | MTR_8g040930 | 64.262 | 305 | 100 | 5 | 52 | 350 | 2 | 303 | 8.48e-131 | 379 |
| MsG0680035403.01.T01 | MTR_8g041240 | 64.262 | 305 | 100 | 5 | 52 | 350 | 2 | 303 | 8.48e-131 | 379 |
| MsG0680035403.01.T01 | MTR_8g467780 | 56.941 | 353 | 141 | 3 | 7 | 348 | 9 | 361 | 6.01e-130 | 379 |
| MsG0680035403.01.T01 | MTR_8g066670 | 61.429 | 350 | 131 | 4 | 1 | 348 | 1067 | 1414 | 8.37e-130 | 405 |
| MsG0680035403.01.T01 | MTR_3g105650 | 54.019 | 311 | 133 | 4 | 7 | 309 | 3 | 311 | 1.67e-110 | 329 |
| MsG0680035403.01.T01 | MTR_8g073275 | 46.721 | 366 | 118 | 7 | 52 | 350 | 2 | 357 | 7.60e-94 | 287 |
| MsG0680035403.01.T01 | MTR_1g069110 | 28.037 | 321 | 206 | 7 | 9 | 324 | 18 | 318 | 8.74e-29 | 116 |
| MsG0680035403.01.T01 | MTR_7g099580 | 25.159 | 314 | 182 | 7 | 7 | 314 | 10 | 276 | 6.91e-25 | 103 |
| MsG0680035403.01.T01 | MTR_7g099570 | 28.718 | 195 | 125 | 3 | 9 | 198 | 9 | 194 | 5.23e-20 | 91.7 |
| MsG0680035403.01.T01 | MTR_2g101320 | 28.241 | 216 | 137 | 4 | 37 | 244 | 134 | 339 | 1.83e-17 | 84.0 |
| MsG0680035403.01.T01 | MTR_7g117620 | 25.260 | 289 | 178 | 6 | 44 | 308 | 119 | 393 | 1.39e-16 | 81.3 |
| MsG0680035403.01.T01 | MTR_1g032100 | 26.460 | 291 | 173 | 8 | 45 | 308 | 95 | 371 | 6.75e-16 | 79.0 |
| MsG0680035403.01.T01 | MTR_1g115575 | 24.830 | 294 | 176 | 7 | 44 | 309 | 169 | 445 | 1.38e-15 | 78.2 |
| MsG0680035403.01.T01 | MTR_8g105260 | 24.742 | 291 | 180 | 7 | 44 | 308 | 101 | 378 | 4.12e-14 | 73.6 |
| MsG0680035403.01.T01 | MTR_2g101310 | 26.291 | 213 | 139 | 4 | 40 | 244 | 137 | 339 | 6.93e-14 | 73.2 |
| MsG0680035403.01.T01 | MTR_2g101310 | 26.291 | 213 | 139 | 4 | 40 | 244 | 137 | 339 | 9.70e-14 | 72.4 |
| MsG0680035403.01.T01 | MTR_7g100390 | 24.620 | 329 | 200 | 9 | 9 | 308 | 53 | 362 | 1.58e-12 | 68.9 |
| MsG0680035403.01.T01 | MTR_4g062100 | 22.569 | 288 | 187 | 4 | 45 | 308 | 109 | 384 | 2.53e-11 | 65.1 |
| MsG0680035403.01.T01 | MTR_7g100430 | 24.224 | 322 | 182 | 9 | 42 | 309 | 124 | 437 | 2.56e-11 | 65.1 |
| MsG0680035403.01.T01 | MTR_5g085480 | 26.829 | 246 | 160 | 6 | 9 | 246 | 214 | 447 | 6.42e-11 | 63.9 |
| MsG0680035403.01.T01 | MTR_2g096190 | 22.867 | 293 | 180 | 6 | 45 | 308 | 105 | 380 | 6.65e-11 | 63.9 |
| MsG0680035403.01.T01 | MTR_5g085480 | 26.829 | 246 | 160 | 6 | 9 | 246 | 54 | 287 | 8.12e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035403.01.T01 | AT4G10720 | 40.625 | 320 | 182 | 2 | 8 | 319 | 4 | 323 | 2.29e-79 | 249 |
| MsG0680035403.01.T01 | AT4G10720 | 40.625 | 320 | 182 | 2 | 8 | 319 | 4 | 323 | 7.86e-79 | 248 |
| MsG0680035403.01.T01 | AT5G15500 | 38.788 | 330 | 194 | 4 | 9 | 330 | 6 | 335 | 3.61e-75 | 239 |
| MsG0680035403.01.T01 | AT5G54620 | 41.424 | 309 | 170 | 4 | 7 | 306 | 3 | 309 | 3.79e-71 | 228 |
| MsG0680035403.01.T01 | AT1G14480 | 37.425 | 334 | 198 | 3 | 8 | 330 | 4 | 337 | 3.82e-69 | 223 |
| MsG0680035403.01.T01 | AT1G14500 | 36.335 | 322 | 196 | 2 | 8 | 320 | 4 | 325 | 2.68e-68 | 221 |
| MsG0680035403.01.T01 | AT5G54610 | 37.377 | 305 | 176 | 5 | 8 | 302 | 4 | 303 | 6.59e-63 | 206 |
| MsG0680035403.01.T01 | AT1G14480 | 33.951 | 324 | 180 | 4 | 8 | 330 | 4 | 294 | 1.62e-51 | 176 |
| MsG0680035403.01.T01 | AT5G15500 | 34.061 | 229 | 143 | 4 | 110 | 330 | 1 | 229 | 1.11e-33 | 127 |
| MsG0680035403.01.T01 | AT5G51160 | 28.328 | 293 | 182 | 4 | 41 | 312 | 13 | 298 | 1.64e-29 | 118 |
| MsG0680035403.01.T01 | AT1G10340 | 27.444 | 266 | 153 | 6 | 79 | 312 | 175 | 432 | 1.34e-17 | 84.3 |
| MsG0680035403.01.T01 | AT1G10340 | 27.444 | 266 | 153 | 6 | 79 | 312 | 171 | 428 | 1.44e-17 | 84.3 |
| MsG0680035403.01.T01 | AT1G34050 | 27.376 | 263 | 163 | 8 | 65 | 312 | 174 | 423 | 3.53e-17 | 83.2 |
| MsG0680035403.01.T01 | AT2G24600 | 27.536 | 276 | 140 | 8 | 79 | 312 | 175 | 432 | 1.35e-16 | 81.3 |
| MsG0680035403.01.T01 | AT2G24600 | 27.536 | 276 | 140 | 8 | 79 | 312 | 175 | 432 | 1.35e-16 | 81.3 |
| MsG0680035403.01.T01 | AT3G09550 | 25.228 | 329 | 194 | 8 | 12 | 309 | 150 | 457 | 1.76e-16 | 81.3 |
| MsG0680035403.01.T01 | AT3G09550 | 25.228 | 329 | 194 | 8 | 12 | 309 | 150 | 457 | 1.76e-16 | 81.3 |
| MsG0680035403.01.T01 | AT2G24600 | 27.536 | 276 | 140 | 8 | 79 | 312 | 175 | 432 | 1.87e-16 | 80.9 |
| MsG0680035403.01.T01 | AT2G24600 | 27.536 | 276 | 140 | 8 | 79 | 312 | 175 | 432 | 1.87e-16 | 80.9 |
| MsG0680035403.01.T01 | AT3G09550 | 25.228 | 329 | 194 | 8 | 12 | 309 | 157 | 464 | 1.89e-16 | 80.9 |
| MsG0680035403.01.T01 | AT4G03500 | 28.287 | 251 | 144 | 7 | 71 | 309 | 276 | 502 | 2.96e-16 | 80.5 |
| MsG0680035403.01.T01 | AT4G03500 | 28.287 | 251 | 144 | 7 | 71 | 309 | 253 | 479 | 3.01e-16 | 80.5 |
| MsG0680035403.01.T01 | AT4G03500 | 28.287 | 251 | 144 | 7 | 71 | 309 | 253 | 479 | 3.01e-16 | 80.5 |
| MsG0680035403.01.T01 | AT2G01680 | 22.319 | 345 | 219 | 7 | 14 | 322 | 57 | 388 | 4.56e-16 | 79.7 |
| MsG0680035403.01.T01 | AT4G03460 | 30.000 | 220 | 117 | 8 | 102 | 309 | 292 | 486 | 8.14e-15 | 75.9 |
| MsG0680035403.01.T01 | AT4G03460 | 30.000 | 220 | 117 | 8 | 102 | 309 | 260 | 454 | 8.95e-15 | 75.9 |
| MsG0680035403.01.T01 | AT5G54710 | 27.863 | 262 | 150 | 8 | 79 | 312 | 246 | 496 | 3.69e-14 | 73.9 |
| MsG0680035403.01.T01 | AT3G12360 | 26.280 | 293 | 173 | 9 | 44 | 309 | 167 | 443 | 3.77e-14 | 73.9 |
| MsG0680035403.01.T01 | AT5G54710 | 27.863 | 262 | 150 | 8 | 79 | 312 | 201 | 451 | 3.91e-14 | 73.9 |
| MsG0680035403.01.T01 | AT3G12360 | 26.280 | 293 | 173 | 9 | 44 | 309 | 155 | 431 | 4.00e-14 | 73.9 |
| MsG0680035403.01.T01 | AT1G05640 | 26.897 | 290 | 172 | 9 | 44 | 308 | 191 | 465 | 6.33e-14 | 73.2 |
| MsG0680035403.01.T01 | AT2G31820 | 25.610 | 328 | 185 | 11 | 21 | 308 | 193 | 501 | 2.11e-12 | 68.6 |
| MsG0680035403.01.T01 | AT1G07710 | 24.000 | 325 | 207 | 7 | 9 | 308 | 70 | 379 | 2.17e-12 | 68.6 |
| MsG0680035403.01.T01 | AT1G07710 | 24.000 | 325 | 207 | 7 | 9 | 308 | 70 | 379 | 2.17e-12 | 68.6 |
| MsG0680035403.01.T01 | AT1G03670 | 26.860 | 242 | 138 | 6 | 86 | 315 | 264 | 478 | 9.07e-12 | 66.6 |
Find 88 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTGGTTTGAAAGAAAA+TGG | 0.181933 | 6:+104746282 | MsG0680035403.01.T01:three_prime_UTR |
| ATCAACAATTCATTTGTAAT+TGG | 0.216753 | 6:+104746029 | MsG0680035403.01.T01:three_prime_UTR |
| GACATTGTTGTCAGACAATT+TGG | 0.217298 | 6:-104746136 | None:intergenic |
| CAGGCCCTTCAAGTACTTCT+TGG | 0.237371 | 6:+104744485 | MsG0680035403.01.T01:CDS |
| TCATAATTTCAGTGGCAAAT+TGG | 0.275256 | 6:-104744178 | None:intergenic |
| CTGGAACTGATCATTCTTAA+TGG | 0.300860 | 6:-104744465 | None:intergenic |
| CTAACCCCAACTGGGATAAT+AGG | 0.331029 | 6:+104745915 | MsG0680035403.01.T01:three_prime_UTR |
| CTTTACACATCGCTGCATCT+AGG | 0.348206 | 6:+104744147 | MsG0680035403.01.T01:CDS |
| CAAAGGACAAACAAAATTAC+TGG | 0.362816 | 6:-104745957 | None:intergenic |
| TCAAACATGAACACATATAA+TGG | 0.362911 | 6:+104744011 | MsG0680035403.01.T01:exon |
| GCTGAAAGCTGCAGCTGAAT+CGG | 0.370537 | 6:+104744040 | MsG0680035403.01.T01:CDS |
| CTGAAAGCTGCAGCTGAATC+GGG | 0.385260 | 6:+104744041 | MsG0680035403.01.T01:CDS |
| ACCCCAGCGTAGTCATATAA+AGG | 0.391742 | 6:-104746064 | None:intergenic |
| GGGGCTCACGATTGATTGAT+AGG | 0.393937 | 6:-104745716 | None:intergenic |
| TCTCACTAATGGGTGAATAT+AGG | 0.395273 | 6:-104743968 | None:intergenic |
| GAGCTGAAACGAAAGGAAAT+CGG | 0.400074 | 6:-104745848 | None:intergenic |
| AAACAAAAGGAAATCATATT+CGG | 0.400593 | 6:-104745870 | None:intergenic |
| ATTTCCCCTGCATCCATCAT+TGG | 0.402618 | 6:+104746002 | MsG0680035403.01.T01:three_prime_UTR |
| GTCAAGCATTCAATGGATTC+CGG | 0.407639 | 6:-104744413 | None:intergenic |
| CCCTTCAAGTACTTCTTGGT+TGG | 0.412618 | 6:+104744489 | MsG0680035403.01.T01:CDS |
| CACCACGATAGAAGACAAAA+TGG | 0.415268 | 6:+104745606 | MsG0680035403.01.T01:CDS |
| ATAGTTTGTCTAACCCCAAC+TGG | 0.417293 | 6:+104745906 | MsG0680035403.01.T01:three_prime_UTR |
| GACCGCCTATTATCCCAGTT+GGG | 0.419656 | 6:-104745920 | None:intergenic |
| CGAGAGAGGTGCAGAAGAGC+TGG | 0.420911 | 6:+104744526 | MsG0680035403.01.T01:CDS |
| AAGCTTTCTCTTTGCTTGTC+CGG | 0.429085 | 6:+104744394 | MsG0680035403.01.T01:CDS |
| CATTAAGAATGATCAGTTCC+AGG | 0.429925 | 6:+104744466 | MsG0680035403.01.T01:CDS |
| CTTCATTATGCAAGTCAGAT+TGG | 0.430540 | 6:+104744353 | MsG0680035403.01.T01:CDS |
| TCTTGGAAGCTAAATGTTGA+AGG | 0.439916 | 6:+104744218 | MsG0680035403.01.T01:CDS |
| TTTACACATCGCTGCATCTA+GGG | 0.447326 | 6:+104744148 | MsG0680035403.01.T01:CDS |
| TCTCACAGTCAAGCATTCAA+TGG | 0.452338 | 6:-104744420 | None:intergenic |
| TTCCTTTATATGACTACGCT+GGG | 0.454370 | 6:+104746062 | MsG0680035403.01.T01:three_prime_UTR |
| GTCAGACAATTTGGCACCAA+TGG | 0.454523 | 6:-104746127 | None:intergenic |
| TGTAAAGGAGTTTCAACAAA+TGG | 0.455867 | 6:-104744131 | None:intergenic |
| TGTAGCACAGGCTCGATTGT+TGG | 0.459562 | 6:+104745423 | MsG0680035403.01.T01:intron |
| GGTAATAATAGTAGAATTTG+AGG | 0.463196 | 6:-104745791 | None:intergenic |
| TGCTGTAATTGATAAACAAA+AGG | 0.464859 | 6:-104745883 | None:intergenic |
| GAATATACGTGTATTAATTG+TGG | 0.469920 | 6:+104746267 | MsG0680035403.01.T01:three_prime_UTR |
| TTTAGCTTCCAAGAAAATGA+AGG | 0.469976 | 6:-104744209 | None:intergenic |
| TTATTACCCCAGAAAATGTT+GGG | 0.478902 | 6:+104745806 | MsG0680035403.01.T01:CDS |
| CCCAGAAAATGTTGGGAGAT+CGG | 0.480038 | 6:+104745813 | MsG0680035403.01.T01:CDS |
| AAGAAAATATTGTTAAGTGC+TGG | 0.481481 | 6:+104745523 | MsG0680035403.01.T01:CDS |
| AGCTGAAACGAAAGGAAATC+GGG | 0.490775 | 6:-104745847 | None:intergenic |
| ATAATACCAATGATGGATGC+AGG | 0.496182 | 6:-104746008 | None:intergenic |
| ATTGTTGATAATACCAATGA+TGG | 0.496513 | 6:-104746015 | None:intergenic |
| ATTATTACCCCAGAAAATGT+TGG | 0.500996 | 6:+104745805 | MsG0680035403.01.T01:CDS |
| AGAATTAATAAGTGAACCAC+AGG | 0.512346 | 6:+104744607 | MsG0680035403.01.T01:CDS |
| AGACCGCCTATTATCCCAGT+TGG | 0.521623 | 6:-104745921 | None:intergenic |
| AAAGACTGTGATAGATTTGA+CGG | 0.526302 | 6:+104745447 | MsG0680035403.01.T01:CDS |
| GCAAGTCAGATTGGTGAGGT+TGG | 0.531080 | 6:+104744362 | MsG0680035403.01.T01:CDS |
| ATCCATCTTGCTATGCAAAA+CGG | 0.531789 | 6:+104744251 | MsG0680035403.01.T01:CDS |
| AAAGAACTTGTTAAAGTGCA+AGG | 0.532557 | 6:+104744311 | MsG0680035403.01.T01:CDS |
| GCCAACCAAGAAGTACTTGA+AGG | 0.535702 | 6:-104744490 | None:intergenic |
| CTCAAAAGAAATTGCGAGAG+AGG | 0.539413 | 6:+104744512 | MsG0680035403.01.T01:CDS |
| TTTCCTTTATATGACTACGC+TGG | 0.543366 | 6:+104746061 | MsG0680035403.01.T01:three_prime_UTR |
| CTAGATGCAGCGATGTGTAA+AGG | 0.543809 | 6:-104744146 | None:intergenic |
| GCAAAACGGTCAAACAATGA+TGG | 0.544713 | 6:+104744265 | MsG0680035403.01.T01:CDS |
| CAAGAAGTACTTGAAGGGCC+TGG | 0.547051 | 6:-104744484 | None:intergenic |
| GTTAAAGTGCAAGGAAGAGA+AGG | 0.550411 | 6:+104744320 | MsG0680035403.01.T01:CDS |
| TTTCAACATCATAATTTCAG+TGG | 0.552567 | 6:-104744186 | None:intergenic |
| ATCTGACTTGCATAATGAAG+AGG | 0.566251 | 6:-104744350 | None:intergenic |
| AAAGAAAGTACTAAATCAGA+AGG | 0.571130 | 6:+104744550 | MsG0680035403.01.T01:CDS |
| TTAGCCTGATAAACTCCACC+AGG | 0.571244 | 6:-104745739 | None:intergenic |
| TTTGCATAGCAAGATGGATG+GGG | 0.573527 | 6:-104744247 | None:intergenic |
| AGTACTAAATCAGAAGGATG+AGG | 0.575608 | 6:+104744556 | MsG0680035403.01.T01:CDS |
| TAGCGGAGCAACGTAACACT+TGG | 0.581213 | 6:+104745662 | MsG0680035403.01.T01:CDS |
| TAATACCAATGATGGATGCA+GGG | 0.582638 | 6:-104746007 | None:intergenic |
| GTATGTCTTAGTTAAACCAT+TGG | 0.583831 | 6:+104746111 | MsG0680035403.01.T01:three_prime_UTR |
| CTAAATCAGAAGGATGAGGC+AGG | 0.587405 | 6:+104744560 | MsG0680035403.01.T01:CDS |
| ACCGCCTATTATCCCAGTTG+GGG | 0.588488 | 6:-104745919 | None:intergenic |
| AGTTTATTTATTAGCAATAG+CGG | 0.590111 | 6:+104743938 | None:intergenic |
| TCTTAGTCTATCTGCAAGTG+TGG | 0.592366 | 6:-104745578 | None:intergenic |
| CTTAGTCTATCTGCAAGTGT+GGG | 0.621240 | 6:-104745577 | None:intergenic |
| TTACACATCGCTGCATCTAG+GGG | 0.628748 | 6:+104744149 | MsG0680035403.01.T01:CDS |
| AATACCAATGATGGATGCAG+GGG | 0.628750 | 6:-104746006 | None:intergenic |
| TTATGCAAGTCAGATTGGTG+AGG | 0.631196 | 6:+104744358 | MsG0680035403.01.T01:CDS |
| CATTGGAAGAAATATATTAG+CGG | 0.634862 | 6:+104745645 | MsG0680035403.01.T01:CDS |
| CCAACCAAGAAGTACTTGAA+GGG | 0.638063 | 6:-104744489 | None:intergenic |
| ATATTCGGAGCTGAAACGAA+AGG | 0.640667 | 6:-104745855 | None:intergenic |
| TAGTTTGTCTAACCCCAACT+GGG | 0.646288 | 6:+104745907 | MsG0680035403.01.T01:three_prime_UTR |
| CCATAGAAAATAGATAGCAA+AGG | 0.646305 | 6:-104745974 | None:intergenic |
| GCCTGATAAACTCCACCAGG+GGG | 0.647842 | 6:-104745736 | None:intergenic |
| TGGGGTACTCTATACATCAA+CGG | 0.652455 | 6:+104746081 | MsG0680035403.01.T01:three_prime_UTR |
| AGCCTGATAAACTCCACCAG+GGG | 0.665796 | 6:-104745737 | None:intergenic |
| TGTAGTTGCAATGTCTAATG+CGG | 0.666105 | 6:-104745491 | None:intergenic |
| ACCCCAACTGGGATAATAGG+CGG | 0.675508 | 6:+104745918 | MsG0680035403.01.T01:three_prime_UTR |
| TAGCCTGATAAACTCCACCA+GGG | 0.682940 | 6:-104745738 | None:intergenic |
| TCCTTTATATGACTACGCTG+GGG | 0.709625 | 6:+104746063 | MsG0680035403.01.T01:three_prime_UTR |
| CCTGATAAACTCCACCAGGG+GGG | 0.741917 | 6:-104745735 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGAAACTTTTAAATATAA+AGG | - | Chr6:104745069-104745088 | None:intergenic | 10.0% |
| !! | AAAGAAAAATATTCCAAAAT+AGG | - | Chr6:104745313-104745332 | None:intergenic | 15.0% |
| !! | AATTAGAAAATAAAACATCA+AGG | + | Chr6:104744692-104744711 | MsG0680035403.01.T01:intron | 15.0% |
| !! | TAAATATAAAGGACTAATTT+AGG | - | Chr6:104745058-104745077 | None:intergenic | 15.0% |
| !! | TTAAAACTTTCAAAACTTAA+AGG | + | Chr6:104745217-104745236 | MsG0680035403.01.T01:intron | 15.0% |
| !!! | ATGTTTTATTTTCTAATTGA+GGG | - | Chr6:104744690-104744709 | None:intergenic | 15.0% |
| !!! | TGTAGTAAAATAATTCATTT+TGG | + | Chr6:104744878-104744897 | MsG0680035403.01.T01:intron | 15.0% |
| !! | AAACAAAAGGAAATCATATT+CGG | - | Chr6:104745873-104745892 | None:intergenic | 20.0% |
| !! | AAGTTTCAAAAGTTTCAAAT+AGG | - | Chr6:104745155-104745174 | None:intergenic | 20.0% |
| !! | ATCAACAATTCATTTGTAAT+TGG | + | Chr6:104746029-104746048 | MsG0680035403.01.T01:three_prime_UTR | 20.0% |
| !! | TCTATATGCTCAAAAATATA+TGG | - | Chr6:104744104-104744123 | None:intergenic | 20.0% |
| !! | TTTAGTCACTATATTTCATA+TGG | + | Chr6:104744752-104744771 | MsG0680035403.01.T01:intron | 20.0% |
| !!! | CATATTTGAAACTTTTGAAA+CGG | + | Chr6:104745098-104745117 | MsG0680035403.01.T01:intron | 20.0% |
| !!! | GATGTTTTATTTTCTAATTG+AGG | - | Chr6:104744691-104744710 | None:intergenic | 20.0% |
| !!! | TGTTTTATTTTCTAATTGAG+GGG | - | Chr6:104744689-104744708 | None:intergenic | 20.0% |
| !!! | TTGAAACTTTTGAAACTTAA+AGG | + | Chr6:104745157-104745176 | MsG0680035403.01.T01:intron | 20.0% |
| ! | AAGAAAATATTGTTAAGTGC+TGG | + | Chr6:104745523-104745542 | MsG0680035403.01.T01:CDS | 25.0% |
| ! | AATTGTGGTTTGAAAGAAAA+TGG | + | Chr6:104746282-104746301 | MsG0680035403.01.T01:three_prime_UTR | 25.0% |
| ! | CATGAACAATCTCTAATAAA+TGG | + | Chr6:104745376-104745395 | MsG0680035403.01.T01:intron | 25.0% |
| ! | CATTGGAAGAAATATATTAG+CGG | + | Chr6:104745645-104745664 | MsG0680035403.01.T01:CDS | 25.0% |
| ! | GAAACTTAAAGGATTAACTT+GGG | + | Chr6:104745168-104745187 | MsG0680035403.01.T01:intron | 25.0% |
| ! | GAATATACGTGTATTAATTG+TGG | + | Chr6:104746267-104746286 | MsG0680035403.01.T01:three_prime_UTR | 25.0% |
| ! | GAGTATTATGTCAAACTAAA+AGG | + | Chr6:104745248-104745267 | MsG0680035403.01.T01:intron | 25.0% |
| ! | GGTAATAATAGTAGAATTTG+AGG | - | Chr6:104745794-104745813 | None:intergenic | 25.0% |
| ! | TCAAACATGAACACATATAA+TGG | + | Chr6:104744011-104744030 | MsG0680035403.01.T01:exon | 25.0% |
| ! | TGAAACTTAAAGGATTAACT+TGG | + | Chr6:104745167-104745186 | MsG0680035403.01.T01:intron | 25.0% |
| ! | TGCTGTAATTGATAAACAAA+AGG | - | Chr6:104745886-104745905 | None:intergenic | 25.0% |
| ! | TTTCAACATCATAATTTCAG+TGG | - | Chr6:104744189-104744208 | None:intergenic | 25.0% |
| !! | ACTTTTCTTCATCAAATTTG+TGG | - | Chr6:104744845-104744864 | None:intergenic | 25.0% |
| !! | CTTTTCTTCATCAAATTTGT+GGG | - | Chr6:104744844-104744863 | None:intergenic | 25.0% |
| !!! | AAAGAAAGTACTAAATCAGA+AGG | + | Chr6:104744550-104744569 | MsG0680035403.01.T01:CDS | 25.0% |
| !!! | ATTGTTGATAATACCAATGA+TGG | - | Chr6:104746018-104746037 | None:intergenic | 25.0% |
| !!! | CCTAATTGAAACTTTTGAAA+CGG | - | Chr6:104745014-104745033 | None:intergenic | 25.0% |
| !!! | TTTTTTTCTCTCTCACTAAT+GGG | - | Chr6:104743981-104744000 | None:intergenic | 25.0% |
| !!! | TTTTTTTTCTCTCTCACTAA+TGG | - | Chr6:104743982-104744001 | None:intergenic | 25.0% |
| AAAGACTGTGATAGATTTGA+CGG | + | Chr6:104745447-104745466 | MsG0680035403.01.T01:CDS | 30.0% | |
| AGAATTAATAAGTGAACCAC+AGG | + | Chr6:104744607-104744626 | MsG0680035403.01.T01:CDS | 30.0% | |
| ATGTGACACTTAATATAGGA+TGG | + | Chr6:104745338-104745357 | MsG0680035403.01.T01:intron | 30.0% | |
| ATTATTACCCCAGAAAATGT+TGG | + | Chr6:104745805-104745824 | MsG0680035403.01.T01:CDS | 30.0% | |
| CAAAGGACAAACAAAATTAC+TGG | - | Chr6:104745960-104745979 | None:intergenic | 30.0% | |
| CCATAGAAAATAGATAGCAA+AGG | - | Chr6:104745977-104745996 | None:intergenic | 30.0% | |
| CCGTTTCAAAAGTTTCAATT+AGG | + | Chr6:104745011-104745030 | MsG0680035403.01.T01:intron | 30.0% | |
| GCAAATGTGACACTTAATAT+AGG | + | Chr6:104745334-104745353 | MsG0680035403.01.T01:intron | 30.0% | |
| GTATGTCTTAGTTAAACCAT+TGG | + | Chr6:104746111-104746130 | MsG0680035403.01.T01:three_prime_UTR | 30.0% | |
| TAATCTAATGTCATTGCACA+AGG | - | Chr6:104744636-104744655 | None:intergenic | 30.0% | |
| TCATAATTTCAGTGGCAAAT+TGG | - | Chr6:104744181-104744200 | None:intergenic | 30.0% | |
| TGTAAAGGAGTTTCAACAAA+TGG | - | Chr6:104744134-104744153 | None:intergenic | 30.0% | |
| TGTGACACTTAATATAGGAT+GGG | + | Chr6:104745339-104745358 | MsG0680035403.01.T01:intron | 30.0% | |
| TTATTACCCCAGAAAATGTT+GGG | + | Chr6:104745806-104745825 | MsG0680035403.01.T01:CDS | 30.0% | |
| ! | GTAATTTTTATGAGTCGCAT+TGG | + | Chr6:104745628-104745647 | MsG0680035403.01.T01:CDS | 30.0% |
| ! | TGTTGAAACCTTCATTTTCT+TGG | + | Chr6:104744201-104744220 | MsG0680035403.01.T01:CDS | 30.0% |
| ! | TTTAGCTTCCAAGAAAATGA+AGG | - | Chr6:104744212-104744231 | None:intergenic | 30.0% |
| !! | AAAGAACTTGTTAAAGTGCA+AGG | + | Chr6:104744311-104744330 | MsG0680035403.01.T01:CDS | 30.0% |
| !! | CCTTTGCTATCTATTTTCTA+TGG | + | Chr6:104745974-104745993 | MsG0680035403.01.T01:three_prime_UTR | 30.0% |
| AGTACTAAATCAGAAGGATG+AGG | + | Chr6:104744556-104744575 | MsG0680035403.01.T01:CDS | 35.0% | |
| CAAACTAAAAGGACCAAAAG+TGG | + | Chr6:104745259-104745278 | MsG0680035403.01.T01:intron | 35.0% | |
| CATTAAGAATGATCAGTTCC+AGG | + | Chr6:104744466-104744485 | MsG0680035403.01.T01:CDS | 35.0% | |
| CTGGAACTGATCATTCTTAA+TGG | - | Chr6:104744468-104744487 | None:intergenic | 35.0% | |
| CTTCATTATGCAAGTCAGAT+TGG | + | Chr6:104744353-104744372 | MsG0680035403.01.T01:CDS | 35.0% | |
| GACATTGTTGTCAGACAATT+TGG | - | Chr6:104746139-104746158 | None:intergenic | 35.0% | |
| GTGACACTTAATATAGGATG+GGG | + | Chr6:104745340-104745359 | MsG0680035403.01.T01:intron | 35.0% | |
| TCATGATTACTTTGTAGCAC+AGG | + | Chr6:104745411-104745430 | MsG0680035403.01.T01:intron | 35.0% | |
| TCTTGGAAGCTAAATGTTGA+AGG | + | Chr6:104744218-104744237 | MsG0680035403.01.T01:CDS | 35.0% | |
| TGACACTTAATATAGGATGG+GGG | + | Chr6:104745341-104745360 | MsG0680035403.01.T01:intron | 35.0% | |
| TGTAGTTGCAATGTCTAATG+CGG | - | Chr6:104745494-104745513 | None:intergenic | 35.0% | |
| TTCCTTTATATGACTACGCT+GGG | + | Chr6:104746062-104746081 | MsG0680035403.01.T01:three_prime_UTR | 35.0% | |
| TTTCCTTTATATGACTACGC+TGG | + | Chr6:104746061-104746080 | MsG0680035403.01.T01:three_prime_UTR | 35.0% | |
| ! | ATACGACGTGATACCTATTT+TGG | + | Chr6:104745297-104745316 | MsG0680035403.01.T01:intron | 35.0% |
| ! | ATCCATCTTGCTATGCAAAA+CGG | + | Chr6:104744251-104744270 | MsG0680035403.01.T01:CDS | 35.0% |
| ! | ATCTGACTTGCATAATGAAG+AGG | - | Chr6:104744353-104744372 | None:intergenic | 35.0% |
| ! | TACCATTTTGTCTTCTATCG+TGG | - | Chr6:104745611-104745630 | None:intergenic | 35.0% |
| ! | TCAAGGCTAAATACCACTTT+TGG | - | Chr6:104745275-104745294 | None:intergenic | 35.0% |
| ! | TCTCACTAATGGGTGAATAT+AGG | - | Chr6:104743971-104743990 | None:intergenic | 35.0% |
| ! | TTTTGCATAGCAAGATGGAT+GGG | - | Chr6:104744251-104744270 | None:intergenic | 35.0% |
| !! | ATAATACCAATGATGGATGC+AGG | - | Chr6:104746011-104746030 | None:intergenic | 35.0% |
| !! | TAATACCAATGATGGATGCA+GGG | - | Chr6:104746010-104746029 | None:intergenic | 35.0% |
| AAGCTTTCTCTTTGCTTGTC+CGG | + | Chr6:104744394-104744413 | MsG0680035403.01.T01:CDS | 40.0% | |
| AGCTGAAACGAAAGGAAATC+GGG | - | Chr6:104745850-104745869 | None:intergenic | 40.0% | |
| ATATTCGGAGCTGAAACGAA+AGG | - | Chr6:104745858-104745877 | None:intergenic | 40.0% | |
| CACCACGATAGAAGACAAAA+TGG | + | Chr6:104745606-104745625 | MsG0680035403.01.T01:CDS | 40.0% | |
| CTCAAAAGAAATTGCGAGAG+AGG | + | Chr6:104744512-104744531 | MsG0680035403.01.T01:CDS | 40.0% | |
| CTTAGTCTATCTGCAAGTGT+GGG | - | Chr6:104745580-104745599 | None:intergenic | 40.0% | |
| GACACTTAATATAGGATGGG+GGG | + | Chr6:104745342-104745361 | MsG0680035403.01.T01:intron | 40.0% | |
| GAGCTGAAACGAAAGGAAAT+CGG | - | Chr6:104745851-104745870 | None:intergenic | 40.0% | |
| GCAAAACGGTCAAACAATGA+TGG | + | Chr6:104744265-104744284 | MsG0680035403.01.T01:CDS | 40.0% | |
| GTTAAAGTGCAAGGAAGAGA+AGG | + | Chr6:104744320-104744339 | MsG0680035403.01.T01:CDS | 40.0% | |
| TCCTTTATATGACTACGCTG+GGG | + | Chr6:104746063-104746082 | MsG0680035403.01.T01:three_prime_UTR | 40.0% | |
| TCTCACAGTCAAGCATTCAA+TGG | - | Chr6:104744423-104744442 | None:intergenic | 40.0% | |
| TCTTAGTCTATCTGCAAGTG+TGG | - | Chr6:104745581-104745600 | None:intergenic | 40.0% | |
| TTATGCAAGTCAGATTGGTG+AGG | + | Chr6:104744358-104744377 | MsG0680035403.01.T01:CDS | 40.0% | |
| TTTACACATCGCTGCATCTA+GGG | + | Chr6:104744148-104744167 | MsG0680035403.01.T01:CDS | 40.0% | |
| TTTGCATAGCAAGATGGATG+GGG | - | Chr6:104744250-104744269 | None:intergenic | 40.0% | |
| ! | ATAGTTTGTCTAACCCCAAC+TGG | + | Chr6:104745906-104745925 | MsG0680035403.01.T01:three_prime_UTR | 40.0% |
| ! | GGTATCACGTCGTATTTTCA+AGG | - | Chr6:104745292-104745311 | None:intergenic | 40.0% |
| ! | GTCAAGCATTCAATGGATTC+CGG | - | Chr6:104744416-104744435 | None:intergenic | 40.0% |
| ! | GTTTTGCATAGCAAGATGGA+TGG | - | Chr6:104744252-104744271 | None:intergenic | 40.0% |
| ! | TAGTTTGTCTAACCCCAACT+GGG | + | Chr6:104745907-104745926 | MsG0680035403.01.T01:three_prime_UTR | 40.0% |
| ! | TGGGGTACTCTATACATCAA+CGG | + | Chr6:104746081-104746100 | MsG0680035403.01.T01:three_prime_UTR | 40.0% |
| !! | AATACCAATGATGGATGCAG+GGG | - | Chr6:104746009-104746028 | None:intergenic | 40.0% |
| !! | CCAACCAAGAAGTACTTGAA+GGG | - | Chr6:104744492-104744511 | None:intergenic | 40.0% |
| ACCCCAGCGTAGTCATATAA+AGG | - | Chr6:104746067-104746086 | None:intergenic | 45.0% | |
| ATTTCCCCTGCATCCATCAT+TGG | + | Chr6:104746002-104746021 | MsG0680035403.01.T01:three_prime_UTR | 45.0% | |
| CCCAGAAAATGTTGGGAGAT+CGG | + | Chr6:104745813-104745832 | MsG0680035403.01.T01:CDS | 45.0% | |
| CCCTTCAAGTACTTCTTGGT+TGG | + | Chr6:104744489-104744508 | MsG0680035403.01.T01:CDS | 45.0% | |
| CTAAATCAGAAGGATGAGGC+AGG | + | Chr6:104744560-104744579 | MsG0680035403.01.T01:CDS | 45.0% | |
| CTAACCCCAACTGGGATAAT+AGG | + | Chr6:104745915-104745934 | MsG0680035403.01.T01:three_prime_UTR | 45.0% | |
| CTAGATGCAGCGATGTGTAA+AGG | - | Chr6:104744149-104744168 | None:intergenic | 45.0% | |
| CTTTACACATCGCTGCATCT+AGG | + | Chr6:104744147-104744166 | MsG0680035403.01.T01:CDS | 45.0% | |
| GTCAGACAATTTGGCACCAA+TGG | - | Chr6:104746130-104746149 | None:intergenic | 45.0% | |
| TAGCCTGATAAACTCCACCA+GGG | - | Chr6:104745741-104745760 | None:intergenic | 45.0% | |
| TCATTGCACAAGGTTACCTG+TGG | - | Chr6:104744626-104744645 | None:intergenic | 45.0% | |
| TTACACATCGCTGCATCTAG+GGG | + | Chr6:104744149-104744168 | MsG0680035403.01.T01:CDS | 45.0% | |
| TTAGCCTGATAAACTCCACC+AGG | - | Chr6:104745742-104745761 | None:intergenic | 45.0% | |
| ! | ACCGATCTCCCAACATTTTC+TGG | - | Chr6:104745817-104745836 | None:intergenic | 45.0% |
| ! | CCGATCTCCCAACATTTTCT+GGG | - | Chr6:104745816-104745835 | None:intergenic | 45.0% |
| ! | CGATCTCCCAACATTTTCTG+GGG | - | Chr6:104745815-104745834 | None:intergenic | 45.0% |
| ! | GACCGTTTTGCATAGCAAGA+TGG | - | Chr6:104744256-104744275 | None:intergenic | 45.0% |
| !! | GCCAACCAAGAAGTACTTGA+AGG | - | Chr6:104744493-104744512 | None:intergenic | 45.0% |
| !!! | GGGAGTATCAGTGACTTTTG+AGG | - | Chr6:104745560-104745579 | None:intergenic | 45.0% |
| !!! | GTATCAGTGACTTTTGAGGC+AGG | - | Chr6:104745556-104745575 | None:intergenic | 45.0% |
| ACCCCAACTGGGATAATAGG+CGG | + | Chr6:104745918-104745937 | MsG0680035403.01.T01:three_prime_UTR | 50.0% | |
| ACCGCCTATTATCCCAGTTG+GGG | - | Chr6:104745922-104745941 | None:intergenic | 50.0% | |
| AGACCGCCTATTATCCCAGT+TGG | - | Chr6:104745924-104745943 | None:intergenic | 50.0% | |
| AGCCTGATAAACTCCACCAG+GGG | - | Chr6:104745740-104745759 | None:intergenic | 50.0% | |
| CAGGCCCTTCAAGTACTTCT+TGG | + | Chr6:104744485-104744504 | MsG0680035403.01.T01:CDS | 50.0% | |
| CTGAAAGCTGCAGCTGAATC+GGG | + | Chr6:104744041-104744060 | MsG0680035403.01.T01:CDS | 50.0% | |
| GACCGCCTATTATCCCAGTT+GGG | - | Chr6:104745923-104745942 | None:intergenic | 50.0% | |
| GCAAGTCAGATTGGTGAGGT+TGG | + | Chr6:104744362-104744381 | MsG0680035403.01.T01:CDS | 50.0% | |
| GCTGAAAGCTGCAGCTGAAT+CGG | + | Chr6:104744040-104744059 | MsG0680035403.01.T01:CDS | 50.0% | |
| GGGGCTCACGATTGATTGAT+AGG | - | Chr6:104745719-104745738 | None:intergenic | 50.0% | |
| TAGCGGAGCAACGTAACACT+TGG | + | Chr6:104745662-104745681 | MsG0680035403.01.T01:CDS | 50.0% | |
| TGTAGCACAGGCTCGATTGT+TGG | + | Chr6:104745423-104745442 | MsG0680035403.01.T01:intron | 50.0% | |
| !! | CAAGAAGTACTTGAAGGGCC+TGG | - | Chr6:104744487-104744506 | None:intergenic | 50.0% |
| CCTGATAAACTCCACCAGGG+GGG | - | Chr6:104745738-104745757 | None:intergenic | 55.0% | |
| GCCTGATAAACTCCACCAGG+GGG | - | Chr6:104745739-104745758 | None:intergenic | 55.0% | |
| CAATCAATCGTGAGCCCCCC+TGG | + | Chr6:104745721-104745740 | MsG0680035403.01.T01:CDS | 60.0% | |
| CCCCCCTGGTGGAGTTTATC+AGG | + | Chr6:104745735-104745754 | MsG0680035403.01.T01:CDS | 60.0% | |
| ! | CGAGAGAGGTGCAGAAGAGC+TGG | + | Chr6:104744526-104744545 | MsG0680035403.01.T01:CDS | 60.0% |
| TCAATCGTGAGCCCCCCTGG+TGG | + | Chr6:104745724-104745743 | MsG0680035403.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 104743950 | 104746310 | 104743950 | ID=MsG0680035403.01;Name=MsG0680035403.01 |
| Chr6 | mRNA | 104743950 | 104746310 | 104743950 | ID=MsG0680035403.01.T01;Parent=MsG0680035403.01;Name=MsG0680035403.01.T01;_AED=0.25;_eAED=0.25;_QI=67|1|1|1|1|1|2|431|352 |
| Chr6 | exon | 104743950 | 104744628 | 104743950 | ID=MsG0680035403.01.T01:exon:11243;Parent=MsG0680035403.01.T01 |
| Chr6 | exon | 104745433 | 104746310 | 104745433 | ID=MsG0680035403.01.T01:exon:11244;Parent=MsG0680035403.01.T01 |
| Chr6 | five_prime_UTR | 104743950 | 104744016 | 104743950 | ID=MsG0680035403.01.T01:five_prime_utr;Parent=MsG0680035403.01.T01 |
| Chr6 | CDS | 104744017 | 104744628 | 104744017 | ID=MsG0680035403.01.T01:cds;Parent=MsG0680035403.01.T01 |
| Chr6 | CDS | 104745433 | 104745879 | 104745433 | ID=MsG0680035403.01.T01:cds;Parent=MsG0680035403.01.T01 |
| Chr6 | three_prime_UTR | 104745880 | 104746310 | 104745880 | ID=MsG0680035403.01.T01:three_prime_utr;Parent=MsG0680035403.01.T01 |
| Gene Sequence |
| Protein sequence |
