AlfalfaGEDB | Msa0057690

M. sativa cultivar zhongmu-4 / Msa0057690

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006996 GO:0008150 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042644 GO:0042646 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057690	XP_003588461.2	71.296	108	24	3	3	104	4	110	1.89e-33	130

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057690	G7I294	71.296	108	24	3	3	104	4	110	9.03e-34	130

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0057690	0.809310	2.015212e-50	-8.615850e-47
Msa0008850	Msa0057690	0.822890	1.859195e-53	-8.615850e-47
Msa0020630	Msa0057690	0.807317	5.363367e-50	-8.615850e-47
Msa0027540	Msa0057690	0.812683	3.748012e-51	-8.615850e-47
Msa0037820	Msa0057690	0.808553	2.927461e-50	-8.615850e-47
Msa0050720	Msa0057690	0.802998	4.299433e-49	-8.615850e-47
Msa0055280	Msa0057690	0.810298	1.235585e-50	-8.615850e-47
Msa0057530	Msa0057690	0.811536	6.665588e-51	-8.615850e-47
Msa0057690	Msa0068410	0.830951	2.199031e-55	-8.615850e-47
Msa0057690	Msa0075310	0.818917	1.526235e-52	-8.615850e-47
Msa0057690	Msa0084680	0.840770	7.117731e-58	-8.615850e-47
Msa0057690	Msa0091480	0.807718	4.407257e-50	-8.615850e-47
Msa0057690	Msa0101060	0.828497	8.699764e-55	-8.615850e-47
Msa0057690	Msa0102900	0.814770	1.300661e-51	-8.615850e-47
Msa0057690	Msa0103160	0.825882	3.679204e-54	-8.615850e-47
Msa0057690	Msa0114250	0.813691	2.252029e-51	-8.615850e-47
Msa0057690	Msa0159750	0.810627	1.049036e-50	-8.615850e-47
Msa0057690	Msa0168100	0.806565	7.733769e-50	-8.615850e-47
Msa0057690	Msa0182930	0.827988	1.154181e-54	-8.615850e-47
Msa0057690	Msa0185390	0.822829	1.920976e-53	-8.615850e-47
Msa0057690	Msa0194570	0.800924	1.147092e-48	-8.615850e-47
Msa0057690	Msa0198440	0.801449	8.957555e-49	-8.615850e-47
Msa0057690	Msa0265210	0.807819	4.194597e-50	-8.615850e-47
Msa0057690	Msa0265580	0.816815	4.551214e-52	-8.615850e-47
Msa0057690	Msa0282170	0.803154	3.992294e-49	-8.615850e-47
Msa0057690	Msa0288240	0.822375	2.450559e-53	-8.615850e-47
Msa0057690	Msa0298970	0.803996	2.670121e-49	-8.615850e-47
Msa0057690	Msa0301180	0.801066	1.073152e-48	-8.615850e-47
Msa0057690	Msa0317260	0.808007	3.825575e-50	-8.615850e-47
Msa0057690	Msa0318070	0.802141	6.457854e-49	-8.615850e-47
Msa0057690	Msa0352510	0.803972	2.701209e-49	-8.615850e-47
Msa0057690	Msa0354390	0.800803	1.214378e-48	-8.615850e-47
Msa0057690	Msa0401340	0.820392	7.028976e-53	-8.615850e-47
Msa0057690	Msa0417630	0.804084	2.559936e-49	-8.615850e-47
Msa0057690	Msa0421800	0.801021	1.096118e-48	-8.615850e-47
Msa0057690	Msa0432680	0.804230	2.386356e-49	-8.615850e-47
Msa0057690	Msa0448080	0.811960	5.389530e-51	-8.615850e-47
Msa0057690	Msa0448120	0.814772	1.299702e-51	-8.615850e-47
Msa0057690	Msa0458790	0.807099	5.962901e-50	-8.615850e-47
Msa0057690	Msa0467800	0.805173	1.516716e-49	-8.615850e-47
Msa0057690	Msa0467880	0.808041	3.762952e-50	-8.615850e-47
Msa0057690	Msa0514260	0.802322	5.928393e-49	-8.615850e-47
Msa0057690	Msa0524050	0.817499	3.195254e-52	-8.615850e-47
Msa0057690	Msa0524090	0.809702	1.660779e-50	-8.615850e-47
Msa0057690	Msa0524120	0.827383	1.612526e-54	-8.615850e-47
Msa0057690	Msa0530950	0.803710	3.060776e-49	-8.615850e-47
Msa0057690	Msa0532840	0.808761	2.642487e-50	-8.615850e-47
Msa0057690	Msa0533010	0.808182	3.511004e-50	-8.615850e-47
Msa0057690	Msa0542040	0.820983	5.140026e-53	-8.615850e-47
Msa0057690	Msa0546740	0.816904	4.346976e-52	-8.615850e-47
Msa0057690	Msa0550110	0.800580	1.348259e-48	-8.615850e-47
Msa0057690	Msa0576420	0.831941	1.254845e-55	-8.615850e-47
Msa0057690	Msa0583740	0.836178	1.089365e-56	-8.615850e-47
Msa0057690	Msa0605620	0.819269	1.269375e-52	-8.615850e-47
Msa0057690	Msa0607300	0.800325	1.519750e-48	-8.615850e-47
Msa0057690	Msa0618010	0.804423	2.175860e-49	-8.615850e-47
Msa0057690	Msa0620640	0.805394	1.363624e-49	-8.615850e-47
Msa0057690	Msa0628100	0.811487	6.832747e-51	-8.615850e-47
Msa0057690	Msa0659040	0.800830	1.198997e-48	-8.615850e-47
Msa0057690	Msa0669220	0.810768	9.779458e-51	-8.615850e-47
Msa0057690	Msa0681890	0.810698	1.012811e-50	-8.615850e-47
Msa0057690	Msa0690350	0.808585	2.881856e-50	-8.615850e-47
Msa0057690	Msa0704220	0.821343	4.247237e-53	-8.615850e-47
Msa0057690	Msa0707780	0.817734	2.827850e-52	-8.615850e-47
Msa0057690	Msa0709480	0.802353	5.841485e-49	-8.615850e-47
Msa0057690	Msa0714600	0.815493	8.985603e-52	-8.615850e-47
Msa0057690	Msa0724050	0.804013	2.648273e-49	-8.615850e-47
Msa0057690	Msa0729550	0.804902	1.728109e-49	-8.615850e-47
Msa0057690	Msa0730180	0.826544	2.559384e-54	-8.615850e-47
Msa0057690	Msa0733000	0.802579	5.246230e-49	-8.615850e-47
Msa0057690	Msa0743650	0.800655	1.301822e-48	-8.615850e-47
Msa0057690	Msa0763880	0.806400	8.380102e-50	-8.615850e-47
Msa0057690	Msa0763910	0.803012	4.270882e-49	-8.615850e-47
Msa0057690	Msa0769830	0.824443	8.049580e-54	-8.615850e-47
Msa0057690	Msa0809300	0.800651	1.304177e-48	-8.615850e-47
Msa0057690	Msa0848760	0.814686	1.357629e-51	-8.615850e-47
Msa0057690	Msa0851740	0.808648	2.793261e-50	-8.615850e-47
Msa0057690	Msa0872660	0.802346	5.859014e-49	-8.615850e-47
Msa0057690	Msa0925160	0.815522	8.854788e-52	-8.615850e-47
Msa0057690	Msa0955260	0.817487	3.215234e-52	-8.615850e-47
Msa0057690	Msa0971860	0.806977	6.327514e-50	-8.615850e-47
Msa0057690	Msa0975080	0.801025	1.094035e-48	-8.615850e-47
Msa0057690	Msa1037060	-0.810089	1.370908e-50	-8.615850e-47
Msa0057690	Msa1038320	0.810458	1.141361e-50	-8.615850e-47
Msa0057690	Msa1055360	0.809992	1.438438e-50	-8.615850e-47
Msa0057690	Msa1072340	0.810014	1.422553e-50	-8.615850e-47
Msa0057690	Msa1118150	0.824770	6.741332e-54	-8.615850e-47
Msa0057690	Msa1125780	0.802964	4.368892e-49	-8.615850e-47
Msa0057690	Msa1125950	0.802036	6.786641e-49	-8.615850e-47
Msa0057690	Msa1140010	0.813355	2.668786e-51	-8.615850e-47
Msa0057690	Msa1159360	0.807564	4.752803e-50	-8.615850e-47
Msa0057690	Msa1164800	-0.808267	3.369146e-50	-8.615850e-47
Msa0057690	Msa1171340	0.810627	1.049466e-50	-8.615850e-47
Msa0057690	Msa1177240	0.840858	6.749095e-58	-8.615850e-47
Msa0057690	Msa1210770	0.808936	2.423503e-50	-8.615850e-47
Msa0057690	Msa1265960	0.809924	1.487695e-50	-8.615850e-47
Msa0057690	Msa1284520	0.803828	2.893002e-49	-8.615850e-47
Msa0057690	Msa1317030	0.827613	1.420052e-54	-8.615850e-47
Msa0057690	Msa1326400	0.805498	1.296976e-49	-8.615850e-47
Msa0057690	Msa1352290	0.830410	2.983334e-55	-8.615850e-47
Msa0057690	Msa1381130	0.824657	7.168571e-54	-8.615850e-47
Msa0057690	Msa1426290	0.802976	4.345169e-49	-8.615850e-47
Msa0057690	Msa1427830	0.813782	2.150031e-51	-8.615850e-47
Msa0057690	Msa1428420	0.833043	6.690463e-56	-8.615850e-47
Msa0057690	Msa1431730	0.807646	4.565671e-50	-8.615850e-47
Msa0057690	Msa1439120	0.805002	1.646912e-49	-8.615850e-47
Msa0057690	Msa1454110	0.832214	1.074147e-55	-8.615850e-47
Msa0057690	Msa1455660	0.801220	9.978334e-49	-8.615850e-47
Msa0057690	Msa1458920	0.819100	1.386736e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0057690	MtrunA17_Chr1g0146581	71.296	108	24	3	3	104	4	110	1.74e-37	130

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACTTTAAATGCTGAAAT+TGG	0.227545	1_2:+13875734	None:intergenic
CAACGTGATGCACTTCTTAA+AGG	0.256123	1_2:-13875580	Msa0057690:CDS
GAAGCAGAAGAATCGATTAT+TGG	0.291262	1_2:-13875713	Msa0057690:CDS
TGAGTGCAAGTTAGTAAGTT+TGG	0.300022	1_2:+13875757	None:intergenic
ACCCTTGTAAGTTCTTTCTT+AGG	0.324828	1_2:-13875520	Msa0057690:CDS
CGGTTAAGAAGTTCGTTAAG+TGG	0.478824	1_2:+13875637	None:intergenic
AGCCTAAGAAAGAACTTACA+AGG	0.515706	1_2:+13875518	None:intergenic
ACAAGTTACGAAGTTGGTGG+TGG	0.531382	1_2:-13875664	Msa0057690:CDS
GGTAATCATTTCTTGAAGAA+CGG	0.532423	1_2:+13875540	None:intergenic
AGCGATACAAGTTACGAAGT+TGG	0.581478	1_2:-13875670	Msa0057690:CDS
AAATGAAAACTCAGAAGAGG+AGG	0.610934	1_2:-13875614	Msa0057690:CDS
CCGAAATGAAAACTCAGAAG+AGG	0.625197	1_2:-13875617	Msa0057690:CDS
CGTGATGCACTTCTTAAAGG+TGG	0.640526	1_2:-13875577	Msa0057690:CDS
GCCTAAGAAAGAACTTACAA+GGG	0.649502	1_2:+13875519	None:intergenic
GATACAAGTTACGAAGTTGG+TGG	0.649679	1_2:-13875667	Msa0057690:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTAAGTTTGGAATATTTTT+AGG	+	chr1_2:13875561-13875580	None:intergenic	20.0%
!!!	TTTTTAGGATAAGTTTGTGT+GGG	+	chr1_2:13875546-13875565	None:intergenic	25.0%
!!!	ATTTTTAGGATAAGTTTGTG+TGG	+	chr1_2:13875547-13875566	None:intergenic	25.0%
!	TCAACTTTAAATGCTGAAAT+TGG	+	chr1_2:13875596-13875615	None:intergenic	25.0%
	GGTAATCATTTCTTGAAGAA+CGG	+	chr1_2:13875790-13875809	None:intergenic	30.0%
	TGAGTGCAAGTTAGTAAGTT+TGG	+	chr1_2:13875573-13875592	None:intergenic	35.0%
	GAAGCAGAAGAATCGATTAT+TGG	-	chr1_2:13875614-13875633	Msa0057690:CDS	35.0%
!	CCTCTTCTGAGTTTTCATTT+CGG	+	chr1_2:13875713-13875732	None:intergenic	35.0%
	AAATGAAAACTCAGAAGAGG+AGG	-	chr1_2:13875713-13875732	Msa0057690:CDS	35.0%
	ACCCTTGTAAGTTCTTTCTT+AGG	-	chr1_2:13875807-13875826	Msa0057690:CDS	35.0%
	GCCTAAGAAAGAACTTACAA+GGG	+	chr1_2:13875811-13875830	None:intergenic	35.0%
	AGCCTAAGAAAGAACTTACA+AGG	+	chr1_2:13875812-13875831	None:intergenic	35.0%
	AGCGATACAAGTTACGAAGT+TGG	-	chr1_2:13875657-13875676	Msa0057690:CDS	40.0%
	GATACAAGTTACGAAGTTGG+TGG	-	chr1_2:13875660-13875679	Msa0057690:CDS	40.0%
	CGGTTAAGAAGTTCGTTAAG+TGG	+	chr1_2:13875693-13875712	None:intergenic	40.0%
	CCGAAATGAAAACTCAGAAG+AGG	-	chr1_2:13875710-13875729	Msa0057690:CDS	40.0%
	CAACGTGATGCACTTCTTAA+AGG	-	chr1_2:13875747-13875766	Msa0057690:CDS	40.0%
!!	ACAAGTTACGAAGTTGGTGG+TGG	-	chr1_2:13875663-13875682	Msa0057690:CDS	45.0%
	CGTGATGCACTTCTTAAAGG+TGG	-	chr1_2:13875750-13875769	Msa0057690:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	13875513	13875836	13875513	ID=Msa0057690;Name=Msa0057690
chr1_2	mRNA	13875513	13875836	13875513	ID=Msa0057690-mRNA-1;Parent=Msa0057690;Name=Msa0057690-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|107
chr1_2	exon	13875513	13875836	13875513	ID=Msa0057690-mRNA-1:exon:5487;Parent=Msa0057690-mRNA-1
chr1_2	CDS	13875513	13875836	13875513	ID=Msa0057690-mRNA-1:cds;Parent=Msa0057690-mRNA-1

Gene Sequence	>Msa0057690 ATGCTTAGCTCTTCAATCTTGTTCACGCTTCCCACACAAACTTATCCTAAAAATATTCCAAACTTACTAACTTGCACTCACCAATTTCAGCATTTAAAGTTGAAGCAGAAGAATCGATTATTGGTGTTAAACAACAGTAGCAGCAGCGATACAAGTTACGAAGTTGGTGGTGGATTTCCACTTAACGAACTTCTTAACCGAAATGAAAACTCAGAAGAGGAGGATACATCTGCTCAACGTGATGCACTTCTTAAAGGTGGTGATCAAGTTATCTCCGTTCTTCAAGAAATGATTACCCTTGTAAGTTCTTTCTTAGGCTTATGA
Protein sequence	>Msa0057690 MLSSSILFTLPTQTYPKNIPNLLTCTHQFQHLKLKQKNRLLVLNNSSSSDTSYEVGGGFPLNELLNRNENSEEEDTSAQRDALLKGGDQVISVLQEMITLVSSFLGL*