AlfalfaGEDB | Msa0076280

M. sativa cultivar zhongmu-4 / Msa0076280

KEGG: K17470|K17471
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008272 GO:0008509 GO:0009507 GO:0009536 GO:0015075 GO:0015103 GO:0015116 GO:0015318 GO:0015698 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0072348 GO:0098656 GO:0098660 GO:0098661 GO:1901682 GO:1902358
PFAM: PF00916.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076280	sp\|Q9SV13\|SUT31_ARATH	74.394	660	163	4	1	656	1	658	0.0	1018

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0076280	G7IDK9	98.628	656	9	0	1	656	1	656	0.0	1336

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0028610	Msa0076280	0.990981	2.728449e-185	-8.615850e-47
Msa0030710	Msa0076280	0.817117	3.894223e-52	-8.615850e-47
Msa0035090	Msa0076280	0.852155	5.557987e-61	-8.615850e-47
Msa0074310	Msa0076280	0.838623	2.575650e-57	-8.615850e-47
Msa0076280	Msa0078110	0.803477	3.422193e-49	-8.615850e-47
Msa0076280	Msa0083200	0.827934	1.189027e-54	-8.615850e-47
Msa0076280	Msa0083570	0.803229	3.850961e-49	-8.615850e-47
Msa0076280	Msa0092750	0.806092	9.728885e-50	-8.615850e-47
Msa0076280	Msa0096230	0.814225	1.717375e-51	-8.615850e-47
Msa0076280	Msa0099830	0.826107	3.252489e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0076280	MtrunA17_Chr1g0185231	98.628	656	9	0	1	656	1	656	1336
Msa0076280	MtrunA17_Chr7g0260181	83.308	659	104	4	1	656	1	656	1095
Msa0076280	MtrunA17_Chr2g0331951	57.387	643	274	0	3	645	5	647	769
Msa0076280	MtrunA17_Chr4g0043931	55.864	648	281	3	4	649	1	645	753
Msa0076280	MtrunA17_Chr5g0425251	55.112	626	280	1	21	645	30	655	747
Msa0076280	MtrunA17_Chr4g0004261	56.230	626	274	0	20	645	29	654	737
Msa0076280	MtrunA17_Chr6g0485191	52.194	638	298	4	15	649	56	689	702
Msa0076280	MtrunA17_Chr3g0115481	54.299	628	283	3	21	645	33	659	701
Msa0076280	MtrunA17_Chr2g0278931	53.409	616	285	2	20	634	34	648	674
Msa0076280	MtrunA17_Chr4g0043951	57.169	544	225	1	10	545	3	546	655

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0076280	AT3G51895.2	74.394	660	163	4	1	656	46	703	1018
Msa0076280	AT3G51895.1	74.394	660	163	4	1	656	1	658	1018
Msa0076280	AT4G02700.1	69.702	637	192	1	21	656	10	646	922
Msa0076280	AT3G15990.1	57.030	633	271	1	12	644	19	650	749
Msa0076280	AT1G23090.1	57.005	621	266	1	20	639	3	623	747
Msa0076280	AT4G08620.1	55.049	614	275	1	26	638	28	641	685
Msa0076280	AT5G19600.1	52.548	628	282	4	21	644	15	630	681
Msa0076280	AT1G78000.2	54.560	625	283	1	14	637	21	645	677
Msa0076280	AT1G78000.1	54.560	625	283	1	14	637	21	645	677
Msa0076280	AT1G78000.3	54.560	625	283	1	14	637	21	645	677

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Find 160 sgRNAs with CRISPR-Local

Find 409 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGATGTAGGAGGAAAT+TGG	0.320530	1_2:+52261723	Msa0076280:CDS
AAACACCTTGCTTATGCATG+TGG	0.583289	1_2:+52254899	Msa0076280:five_prime_UTR
AAAGAGATGAATCAGAGGGT+TGG	0.561882	1_2:+52261807	Msa0076280:CDS
AAATTGGATCTATCTGACAG+TGG	0.706142	1_2:+52261739	Msa0076280:CDS
AACAAAGAGATGATAGCTAT+TGG	0.443400	1_2:+52260477	Msa0076280:CDS
AACCTCCTCAATGGATCATC+TGG	0.355664	1_2:-52255138	None:intergenic
AACGTTGATGAGAGTGTGCA+TGG	0.657132	1_2:+52255048	Msa0076280:CDS
AACTCACATAGTCCAAGAAT+TGG	0.411726	1_2:-52255339	None:intergenic
AAGAACTCAACAACTTATGT+AGG	0.500061	1_2:-52262042	None:intergenic
AAGAACTGAAAAGTGTAGCT+TGG	0.541491	1_2:-52255231	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGACTTCAAAAATGGTTG+AGG	-	chr1_2:52255090-52255109	None:intergenic	30.0%
TTGAATTGTGACTAGATGAA+TGG	-	chr1_2:52255577-52255596	None:intergenic	30.0%
ATCATTGCATAAATTAACGG+TGG	-	chr1_2:52256249-52256268	None:intergenic	30.0%
ACCGTTAATTTATGCAATGA+TGG	+	chr1_2:52256248-52256267	Msa0076280:CDS	30.0%
CCCATCATTGCATAAATTAA+CGG	-	chr1_2:52256252-52256271	None:intergenic	30.0%
CCGTTAATTTATGCAATGAT+GGG	+	chr1_2:52256249-52256268	Msa0076280:CDS	30.0%
TGTTAGTATGTTACTGGATA+TGG	+	chr1_2:52256532-52256551	Msa0076280:intron	30.0%
ACAAGACAACACAAAAAGAA+GGG	-	chr1_2:52256631-52256650	None:intergenic	30.0%
TCTGAATTAAGGCATATACT+TGG	+	chr1_2:52256744-52256763	Msa0076280:intron	30.0%
ATAAATAGCAACGTTCGAAA+GGG	-	chr1_2:52256988-52257007	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	52254750	52262192	52254750	ID=Msa0076280;Name=Msa0076280
chr1_2	mRNA	52254750	52262192	52254750	ID=Msa0076280-mRNA-1;Parent=Msa0076280;Name=Msa0076280-mRNA-1;_AED=0.07;_eAED=0.07;_QI=256\|1\|1\|1\|1\|1\|12\|351\|656
chr1_2	exon	52254750	52255354	52254750	ID=Msa0076280-mRNA-1:exon:16097;Parent=Msa0076280-mRNA-1
chr1_2	exon	52256232	52256439	52256232	ID=Msa0076280-mRNA-1:exon:16098;Parent=Msa0076280-mRNA-1
chr1_2	exon	52257240	52257411	52257240	ID=Msa0076280-mRNA-1:exon:16099;Parent=Msa0076280-mRNA-1
chr1_2	exon	52257681	52257746	52257681	ID=Msa0076280-mRNA-1:exon:16100;Parent=Msa0076280-mRNA-1
chr1_2	exon	52258261	52258374	52258261	ID=Msa0076280-mRNA-1:exon:16101;Parent=Msa0076280-mRNA-1
chr1_2	exon	52259259	52259375	52259259	ID=Msa0076280-mRNA-1:exon:16102;Parent=Msa0076280-mRNA-1
chr1_2	exon	52260420	52260546	52260420	ID=Msa0076280-mRNA-1:exon:16103;Parent=Msa0076280-mRNA-1
chr1_2	exon	52260638	52260924	52260638	ID=Msa0076280-mRNA-1:exon:16104;Parent=Msa0076280-mRNA-1
chr1_2	exon	52261008	52261198	52261008	ID=Msa0076280-mRNA-1:exon:16105;Parent=Msa0076280-mRNA-1
chr1_2	exon	52261303	52261388	52261303	ID=Msa0076280-mRNA-1:exon:16106;Parent=Msa0076280-mRNA-1
chr1_2	exon	52261478	52261554	52261478	ID=Msa0076280-mRNA-1:exon:16107;Parent=Msa0076280-mRNA-1
chr1_2	exon	52261665	52262192	52261665	ID=Msa0076280-mRNA-1:exon:16108;Parent=Msa0076280-mRNA-1
chr1_2	five_prime_UTR	52254750	52255005	52254750	ID=Msa0076280-mRNA-1:five_prime_utr;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52255006	52255354	52255006	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52256232	52256439	52256232	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52257240	52257411	52257240	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52257681	52257746	52257681	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52258261	52258374	52258261	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52259259	52259375	52259259	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52260420	52260546	52260420	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52260638	52260924	52260638	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52261008	52261198	52261008	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52261303	52261388	52261303	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52261478	52261554	52261478	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	CDS	52261665	52261841	52261665	ID=Msa0076280-mRNA-1:cds;Parent=Msa0076280-mRNA-1
chr1_2	three_prime_UTR	52261842	52262192	52261842	ID=Msa0076280-mRNA-1:three_prime_utr;Parent=Msa0076280-mRNA-1

Gene Sequence

>Msa0076280

ATGGGAAACGTAGACTATGATGATTACCCTTGTTCGGGTATGAACGTTGATGAGAGTGTGCATGGTGTGGCAATTCCACCTCCTCAACCATTTTTGAAGTCTATGAAGTATTCAATGAAAGAGACTTTCTTTCCAGATGATCCATTGAGGAGGTTTAAGAATCAACCAGCTTCTAAAAAGTTGGTGCTTGGTCTTCAGTATTTTTTCCCCATATTTGAATGGGCACCAAGCTACACTTTTCAGTTCTTGAAATCTGATCTCATAGCTGGCATCACTATTGCTAGCTTGGCTATTCCTCAAGGCATTAGCTATGCCAAACTTGCCAACCTTCCTCCAATTCTTGGACTATATTCAAGCTTTATACCACCGTTAATTTATGCAATGATGGGTAGTTCAAGGGATTTGGCAGTTGGAACTGTGGCAGTTGGATCTCTTCTGATGGGTTCCATGTTGGCCAATGAGGTTAATCCTACCCAAAATCCTAAGCTTTTTCTCCACCTTGCTTTCACAGCTACATTCTTTGCTGGACTTTTGCAAGCTTCATTGGGTCTCTTTAGGTTAGGGTTTATCGTGGATTTCCTGTCACATGCAGCCATAGTAGGGTTCATGGGAGGAGCAGCTACAGTGGTTTGTCTACAGCAGCTGAAATCAATTCTAGGACTTGAACATTTCACCCATGCTGCTGATATCGTATCCGTCATGCGATCCATTTTTACCCAAACTCATCAGTGGAGGTGGGAAAGCGCTGTGTTGGGATTTTGCTTCATTTTCTTTCTCCTTGTTACAAGATACTTCAGCAAGAAACAACCGAGGTTTTTTTGGGTGTCAGCAATGACGCCGTTGGCGTCCGTTATACTGGGAAGTGTGCTGGTTTATTTCACTCACGCCGAGCACCACGGTGTTCAAGTGATAGGAGAATTGAAGAAAGGACTAAATCCACCATCACTCACAGATTTGGTATTTGTGTCACCATATATGACCACAGCTATCAAAACTGGCCTTATTGTTGGCATTATAGCTCTTGCGGAAGGAATAGCTGTGGGAAGAAGCTTTGCAATGTATAAAAATTATCATATTGATGGCAACAAAGAGATGATAGCTATTGGAACCATGAACATAGTTGGTTCTTTCACCTCTTGCTACCTCACAACTGGGCCATTTTCGCGTTCGGCCGTGAACTATAATGCTGGATGCAAGACAGCAGCATCCAACATTGTGATGTCAATTGCAGTCATGTTGACACTGTTGTTCTTGACACCTTTGTTCTATTACACTCCTCTGGTGGTACTAGCAGCCATAATTGTATCTGCAATGCTTGGACTTATAGATTATGAAGCAGCCATCCATCTCTGGAAGATTGACAAATTCGATTTTTTCGTCTGCATTAGCGCATATATGGGCGTTGTCTTTGGCAGTGTTGAAATTGGCTTAGTCATAGCAGTTGCAATATCAGTGCTTCGGTTACTACTATTTGTTGCAAGGCCAAGGACATTTGTTTTGGGAAACATTCCAAATTCTGTAATATACAGAAACATTGAGCATTATCCGAATGCAAATCGTGTTTCCGGAATTCTAATTCTAAAGATTGATGCACCAATTTACTTTGCCAATGCAAGCTATTTAAGAGAGAGGATCTCAAGGTGGATCGATGAAGAGGAAGACAGAATTAAAGATACAGGGGAGACAATTTTGAATTATGTTATACTGGATATGAGTGCTGTTGGAAATATTGATACGAGTGGAATAAGTATGCTTGAAGAGACTAAAAAGATGGCTGAAAGAAGAGAGCTGCAACTTGTTTTGGTCAATCCTGGAAGTGAGGTGATGAAGAAACTCAACAAATCCAGCTTCCAAAAGGATGTAGGAGGAAATTGGATCTATCTGACAGTGGAAGATGCTGTTAGAGCATGTAACTTTGCATGCAAAACAAATCCCAAAAGAGATGAATCAGAGGGTTGGAACAATGTCTGA

Protein sequence

>Msa0076280

MGNVDYDDYPCSGMNVDESVHGVAIPPPQPFLKSMKYSMKETFFPDDPLRRFKNQPASKKLVLGLQYFFPIFEWAPSYTFQFLKSDLIAGITIASLAIPQGISYAKLANLPPILGLYSSFIPPLIYAMMGSSRDLAVGTVAVGSLLMGSMLANEVNPTQNPKLFLHLAFTATFFAGLLQASLGLFRLGFIVDFLSHAAIVGFMGGAATVVCLQQLKSILGLEHFTHAADIVSVMRSIFTQTHQWRWESAVLGFCFIFFLLVTRYFSKKQPRFFWVSAMTPLASVILGSVLVYFTHAEHHGVQVIGELKKGLNPPSLTDLVFVSPYMTTAIKTGLIVGIIALAEGIAVGRSFAMYKNYHIDGNKEMIAIGTMNIVGSFTSCYLTTGPFSRSAVNYNAGCKTAASNIVMSIAVMLTLLFLTPLFYYTPLVVLAAIIVSAMLGLIDYEAAIHLWKIDKFDFFVCISAYMGVVFGSVEIGLVIAVAISVLRLLLFVARPRTFVLGNIPNSVIYRNIEHYPNANRVSGILILKIDAPIYFANASYLRERISRWIDEEEDRIKDTGETILNYVILDMSAVGNIDTSGISMLEETKKMAERRELQLVLVNPGSEVMKKLNKSSFQKDVGGNWIYLTVEDAVRACNFACKTNPKRDESEGWNNV*