AlfalfaGEDB | Msa0866960

M. sativa cultivar zhongmu-4 / Msa0866960

KEGG: K01535
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF00122.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866960	XP_003603532.1	92.324	951	8	7	1	886	1	951	0.0	1748

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866960	sp\|Q03194\|PMA4_NICPL	81.876	949	107	7	3	886	4	952	0.0	1533

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866960	G7JCD0	92.324	951	8	7	1	886	1	951	0.0	1748

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003490	Msa0866960	0.802671	5.023487e-49	-8.615850e-47
Msa0008330	Msa0866960	0.802044	6.761845e-49	-8.615850e-47
Msa0015370	Msa0866960	0.833024	6.762991e-56	-8.615850e-47
Msa0023150	Msa0866960	0.836385	9.651562e-57	-8.615850e-47
Msa0026120	Msa0866960	0.805192	1.503021e-49	-8.615850e-47
Msa0028610	Msa0866960	0.831789	1.367872e-55	-8.615850e-47
Msa0033600	Msa0866960	0.824435	8.083049e-54	-8.615850e-47
Msa0033650	Msa0866960	0.824845	6.474064e-54	-8.615850e-47
Msa0035090	Msa0866960	0.852724	3.825456e-61	-8.615850e-47
Msa0040190	Msa0866960	0.824538	7.643788e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0866960	MtrunA17_Chr3g0138931	92.324	951	8	7	1	886	1	951	1748
Msa0866960	MtrunA17_Chr1g0148031	86.120	951	67	7	1	886	1	951	1605
Msa0866960	MtrunA17_Chr4g0072131	81.224	948	111	8	4	886	7	952	1529
Msa0866960	MtrunA17_Chr2g0298031	80.882	952	115	9	2	886	7	958	1505
Msa0866960	MtrunA17_Chr8g0365021	78.359	975	120	9	1	886	71	1043	1484
Msa0866960	MtrunA17_Chr1g0147981	81.303	936	109	8	16	886	33	967	1483
Msa0866960	MtrunA17_Chr2g0333721	77.930	947	144	7	5	886	3	949	1457
Msa0866960	MtrunA17_Chr4g0065481	76.321	946	155	8	6	886	7	948	1422
Msa0866960	MtrunA17_Chr4g0059961	75.499	951	163	9	5	886	9	958	1392
Msa0866960	MtrunA17_Chr6g0452721	73.502	951	182	9	5	886	7	956	1372

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0866960	AT2G24520.2	80.084	949	124	7	3	886	46	994	1518
Msa0866960	AT2G24520.3	80.344	931	118	7	21	886	1	931	1497
Msa0866960	AT2G24520.1	80.344	931	118	7	21	886	1	931	1497
Msa0866960	AT2G18960.1	80.021	946	124	7	6	886	4	949	1467
Msa0866960	AT3G42640.1	78.881	947	131	9	5	886	6	948	1466
Msa0866960	AT2G07560.1	78.376	948	137	9	4	886	5	949	1464
Msa0866960	AT4G30190.1	79.937	947	124	8	5	886	3	948	1464
Msa0866960	AT5G57350.4	79.175	946	131	8	6	886	5	949	1453
Msa0866960	AT5G57350.2	79.175	946	131	8	6	886	5	949	1453
Msa0866960	AT5G57350.1	79.175	946	131	8	6	886	5	949	1453

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Find 250 sgRNAs with CRISPR-Local

Find 456 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGTTGGCATAGCAAT+TGG	0.400400	5_4:+69041915	None:intergenic
AAAATCAAACTTCCAGATCA+AGG	0.513418	5_4:+69039971	None:intergenic
AAACATATATGTGGAATGAC+AGG	0.500678	5_4:-69040442	Msa0866960:CDS
AAAGAGAATGACAGCTATTG+AGG	0.476115	5_4:-69041838	Msa0866960:CDS
AAAGCAGTTCTCTTGTCTAC+AGG	0.423643	5_4:+69041361	None:intergenic
AAAGGACCAGCTACGGGACC+TGG	0.558086	5_4:+69039469	None:intergenic
AAAGGCATGCGACCACAATT+TGG	0.235638	5_4:-69037753	Msa0866960:three_prime_UTR
AACAAATAGTTTACCTGCTC+AGG	0.416210	5_4:+69041289	None:intergenic
AACAGACCAACAAATTGCCA+TGG	0.640731	5_4:+69040949	None:intergenic
AACATTATCTGGTATATATT+AGG	0.238496	5_4:-69037441	Msa0866960:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATGGAACAATATAAATGCA+GGG	+	chr5_4:69037452-69037471	None:intergenic	30.0%
TCATGGAACAATATAAATGC+AGG	+	chr5_4:69037453-69037472	None:intergenic	30.0%
TAGCATGTTCATCATTCAAA+TGG	+	chr5_4:69037750-69037769	None:intergenic	30.0%
TGTTTAATGACACACAACTT+AGG	+	chr5_4:69038435-69038454	None:intergenic	30.0%
CAAACAAATTGGAAGAGAAA+AGG	-	chr5_4:69038830-69038849	Msa0866960:intron	30.0%
TGAAGTTCTTAGGATTTATG+TGG	-	chr5_4:69038866-69038885	Msa0866960:intron	30.0%
TTCATTGAAGAGAATAATGC+TGG	-	chr5_4:69039021-69039040	Msa0866960:CDS	30.0%
GCATATAACCTTCAAAATTG+TGG	+	chr5_4:69039264-69039283	None:intergenic	30.0%
AAGTGAAGGACTTAATTGAT+TGG	-	chr5_4:69039371-69039390	Msa0866960:intron	30.0%
AATCAAAGTGAATAGTGCTA+GGG	-	chr5_4:69039645-69039664	Msa0866960:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	69037346	69044940	69037346	ID=Msa0866960;Name=Msa0866960
chr5_4	mRNA	69037346	69044940	69037346	ID=Msa0866960-mRNA-1;Parent=Msa0866960;Name=Msa0866960-mRNA-1;_AED=0.09;_eAED=0.09;_QI=206\|0.61\|0.63\|1\|0.66\|0.63\|19\|865\|886
chr5_4	exon	69037346	69038307	69037346	ID=Msa0866960-mRNA-1:exon:21836;Parent=Msa0866960-mRNA-1
chr5_4	exon	69038422	69038603	69038422	ID=Msa0866960-mRNA-1:exon:21835;Parent=Msa0866960-mRNA-1
chr5_4	exon	69039012	69039140	69039012	ID=Msa0866960-mRNA-1:exon:21834;Parent=Msa0866960-mRNA-1
chr5_4	exon	69039418	69039591	69039418	ID=Msa0866960-mRNA-1:exon:21833;Parent=Msa0866960-mRNA-1
chr5_4	exon	69039681	69039841	69039681	ID=Msa0866960-mRNA-1:exon:21832;Parent=Msa0866960-mRNA-1
chr5_4	exon	69039931	69040012	69039931	ID=Msa0866960-mRNA-1:exon:21831;Parent=Msa0866960-mRNA-1
chr5_4	exon	69040146	69040178	69040146	ID=Msa0866960-mRNA-1:exon:21830;Parent=Msa0866960-mRNA-1
chr5_4	exon	69040264	69040502	69040264	ID=Msa0866960-mRNA-1:exon:21829;Parent=Msa0866960-mRNA-1
chr5_4	exon	69040613	69040777	69040613	ID=Msa0866960-mRNA-1:exon:21828;Parent=Msa0866960-mRNA-1
chr5_4	exon	69040863	69041007	69040863	ID=Msa0866960-mRNA-1:exon:21827;Parent=Msa0866960-mRNA-1
chr5_4	exon	69041106	69041210	69041106	ID=Msa0866960-mRNA-1:exon:21826;Parent=Msa0866960-mRNA-1
chr5_4	exon	69041303	69041425	69041303	ID=Msa0866960-mRNA-1:exon:21825;Parent=Msa0866960-mRNA-1
chr5_4	exon	69041659	69041751	69041659	ID=Msa0866960-mRNA-1:exon:21824;Parent=Msa0866960-mRNA-1
chr5_4	exon	69041773	69041980	69041773	ID=Msa0866960-mRNA-1:exon:21823;Parent=Msa0866960-mRNA-1
chr5_4	exon	69041987	69042179	69041987	ID=Msa0866960-mRNA-1:exon:21822;Parent=Msa0866960-mRNA-1
chr5_4	exon	69042198	69042376	69042198	ID=Msa0866960-mRNA-1:exon:21821;Parent=Msa0866960-mRNA-1
chr5_4	exon	69043198	69043415	69043198	ID=Msa0866960-mRNA-1:exon:21820;Parent=Msa0866960-mRNA-1
chr5_4	exon	69043467	69043553	69043467	ID=Msa0866960-mRNA-1:exon:21819;Parent=Msa0866960-mRNA-1
chr5_4	exon	69044687	69044940	69044687	ID=Msa0866960-mRNA-1:exon:21818;Parent=Msa0866960-mRNA-1
chr5_4	five_prime_UTR	69044735	69044940	69044735	ID=Msa0866960-mRNA-1:five_prime_utr;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69044687	69044734	69044687	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69043467	69043553	69043467	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69043198	69043415	69043198	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69042198	69042376	69042198	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69041987	69042179	69041987	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69041773	69041980	69041773	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69041659	69041751	69041659	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69041303	69041425	69041303	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69041106	69041210	69041106	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69040863	69041007	69040863	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69040613	69040777	69040613	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69040264	69040502	69040264	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69040146	69040178	69040146	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69039931	69040012	69039931	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69039681	69039841	69039681	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69039418	69039591	69039418	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69039012	69039140	69039012	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69038422	69038603	69038422	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	CDS	69038211	69038307	69038211	ID=Msa0866960-mRNA-1:cds;Parent=Msa0866960-mRNA-1
chr5_4	three_prime_UTR	69037346	69038210	69037346	ID=Msa0866960-mRNA-1:three_prime_utr;Parent=Msa0866960-mRNA-1

Gene Sequence

>Msa0866960

ATGGGAGGTATCAGCCTTGAGGAGATAAAAAACGAGAACGTTGATCTGGAACGAATTCCAGTAGAGGAAGTGTTCGAGCAGCTGAAATGTTCAAGAGCAGGTTTAACATCAGACGAAGGAGCCAACAGGCTTCAATTCTTAGGATTTATGTGGAACCCTTTATCATGGGTTATGGAAGCTGCTGCTATTATGGCCATTGCTTTGGCTAATGGCGGTGGAAGACCTCCTGATTGGCAGGATTTTGTTGGTATCATTTCTCTTTTGGTTATTAACTCAACTATCAGTTTCATTGAAGAGAATAATGCTGGTAATGCTGCTGCTGCTCTCATGGCTGGTTTAGCCCCCAAACAAGGGTGGTCTGAAGAAGATGCTGCAATTTTGGTCCCAGGTGATATCATCAGCATTAAATTAGGAGATATCATTCCTGCTGATGCTCGTCTGCTTGAGGGTGATGCTTTGAGTGTTGATCAGTCTGCTTTAACCGGAGAATCTCTTCCGGCAACAAAGAATCCCTCAGATGAAGTGTTTTCTGGTGAGATTGAAGCAGTTGTCATTGCTACTGGAGTGCACACATTTTTTGGTAAAGCTGCTCATTTGGTTGACAGCACCAATCAAGTTGGTCACTTTCAGAAAGTCCTAACAGCAATTGGTAATTTCTGCATTTGCTCAATTGCTGTTGGAATACTGATTGAGCTCGTTGTCATGTATCCGATACAACACCGCAAAGACGGAATTGACAATCTTTTGGTTCTATTGATTGGAGGAATTCCAATTGCTATGCCAACTGTTTTGTCTGTTACCATGGCTATTGGTTCTCACAGACTTTCTCAACAAGGTGCTATTACAAAGAGAATGACAGCTATTGAGGAAATGGCAGGGATGGATGTCCTCTGCAGTGACAAAACCGGAACTCTGACTTTAAATAAGTTATCTGTTTTTGCTAAGGGTGTAGAGAAGGATTATGTTATCCTTCTTGCAGCAAGGGCTTCTAGGACTGAGAATCAGGATGCCATAGATGCTGCAATTGCACGAGCTGGTGTCAGGGAGGTTCATTTTTTCCCATTCAATCCTGTAGACAAGAGAACTGCTTTAACTTACATTGACTCTGACGGAAATTGGCATAGATCTAGCAAAGGTGCTCCTGAGCAGATATTGAACCTCTGCAACTGCAAAGAGGATGTCAGGAAAAAAGCTCATTCAACAATTGACAAGTTCGCCGAGCGTGGACTCCGTTCTTTGGGTGTTGCTAGACAGGAAATACCTGAGAAAGATAAAGATAGCCCTGGTGCACCATGGCAATTTGTTGGTCTGTTACCTCTATTTGATCCTCCTAGGCATGATAGTGCTGAAACCATCACAAGAGCTCTGAACCTTGGTGTGAATGTTAAGATGATTACTGGTGATCAGCTTGCCATTGCCAAGGAAACTGGCCGAAGGCTTGGAATGGGAACAAACATGTATCCATCTTCTTCATTGCTCGGTCAAAGCAAGGATGCTGCTGTTTCAGCTCTTCCAGTTGATGAGTTGATTGAGAAGGCTGATGGATTTGCTGGAGTTTTTCCTGAACACAAGTATGAAATCGTAAAGAAACTGCAAGAGAGGAAACATATATGTGGAATGACAGGTGATGGTGTCAACGACGCTCCTGCGTTAAAGAGAGCAGATATTGGAATTGCTGTTGCTGATGCTACAGATGCCGCTAGAGGTGCTTCTGATATTGTCCTTACTGAGCCTGGTCTGAGTGTCATTATTAGTGCAGTGCTCACAAGCAGAGCAATTTTCCAAAGGATGAAGAACTATACTATCTACGCTGTGTCAATTACCATTCGTATTGTGTTTGGTTTCATGTTCATTGCCTTGATCTGGAAGTTTGATTTTGCACCCTTCATGGTGTTGATTATCGCCATACTAAATGATGGTACCATTATGACAATATCCAAGGATAGAGTGAAACCATCTCCACAACCAGACAGTTGGAAACTGAGAGAGATATTTGCTACTGGTGTTGTGCTAGGTAGCTACATGGCACTAATGACAGTGGTGTTTTTCTGGCTCATGAAGGATACCGATTTCTTCTCGGACAAGTTTGGTGTGAGATCTATCCGAAAAAACCCTGATGAAATGATGGCAGCCCTCTACCTACAAGTCAGTATTATAAGTCAGGCACTAATTTTTGTCACCAGGTCCCGTAGCTGGTCCTTTGTTGAAAGACCTGGTCTTCTCCTATTGGGTGCTTTTATGATTGCTCAGCTGGTGGCAACTTTTATAGCAGTATATGCTAATTGGAGCTTTGCAAGAATTAAGGGAATGGGATGGGGTTGGGCTGGTGTAATCTGGATGTACAGTATAGTGACATATATCCCCCTTGATATACTCAAATTCACTGCTTTCACAACAAAGAAAGACTATGGCAAAGAAGAGAGAGAAGCTCAGTGGGCAACTGCTCAGAGGACTCTTCATGGTCTTCAGTCACCTGACACAACCAACCTTTTCAATGACAAGAATAGCTACAGGGAGCTTTCTGAGATTGCTGAGCAAGCCAAGAGACGTGCTGAAGTTGCACGGCTTAGGGAGCTTCACACTCTAAAGGGTCATGTCGAATCAGTCGTGAAGCTTAAGGGACTTGATATCGACACTATGCAACAACATTATACTGTTTAA

Protein sequence

>Msa0866960

MGGISLEEIKNENVDLERIPVEEVFEQLKCSRAGLTSDEGANRLQFLGFMWNPLSWVMEAAAIMAIALANGGGRPPDWQDFVGIISLLVINSTISFIEENNAGNAAAALMAGLAPKQGWSEEDAAILVPGDIISIKLGDIIPADARLLEGDALSVDQSALTGESLPATKNPSDEVFSGEIEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCICSIAVGILIELVVMYPIQHRKDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVFAKGVEKDYVILLAARASRTENQDAIDAAIARAGVREVHFFPFNPVDKRTALTYIDSDGNWHRSSKGAPEQILNLCNCKEDVRKKAHSTIDKFAERGLRSLGVARQEIPEKDKDSPGAPWQFVGLLPLFDPPRHDSAETITRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSSSLLGQSKDAAVSALPVDELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKRADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMFIALIWKFDFAPFMVLIIAILNDGTIMTISKDRVKPSPQPDSWKLREIFATGVVLGSYMALMTVVFFWLMKDTDFFSDKFGVRSIRKNPDEMMAALYLQVSIISQALIFVTRSRSWSFVERPGLLLLGAFMIAQLVATFIAVYANWSFARIKGMGWGWAGVIWMYSIVTYIPLDILKFTAFTTKKDYGKEEREAQWATAQRTLHGLQSPDTTNLFNDKNSYRELSEIAEQAKRRAEVARLRELHTLKGHVESVVKLKGLDIDTMQQHYTV*