AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0598720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0598720 MtrunA17_Chr4g0022511 97.403 154 4 0 1 154 1 154 1.55e-111 313
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0598720 AT1G03650.1 69.079 152 44 1 6 154 6 157 1.10e-74 220

Find 41 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCAACGGTGACAATATTT+AGG 0.161108 4_2:+36135077 None:intergenic
CAGGTTATGTCATGTATTCT+TGG 0.193464 4_2:-36134827 Msa0598720:CDS
AGTGGAAGAGATAGTGAAAT+TGG 0.247431 4_2:-36134955 Msa0598720:CDS
CACGTTGATGCCCAACTTGC+AGG 0.261704 4_2:-36134846 Msa0598720:CDS
TTAAACAAACACAATATTAC+AGG 0.325566 4_2:+36133520 None:intergenic
CATGTTGACTGTTTGGTTGA+AGG 0.355783 4_2:-36133701 Msa0598720:CDS
GAAGCGAGTGATTCGTGTTT+GGG 0.367842 4_2:+36134918 None:intergenic
GGAAGCGAGTGATTCGTGTT+TGG 0.373847 4_2:+36134917 None:intergenic
TGGCTTTCATGTTGACTGTT+TGG 0.383570 4_2:-36133708 Msa0598720:CDS
TGCAACGGTGACAATATTTA+GGG 0.394824 4_2:+36135078 None:intergenic
CATGACATAACCTGCAAGTT+GGG 0.434152 4_2:+36134836 None:intergenic
TGGACTTCGATTCAAATTGA+AGG 0.474763 4_2:-36133640 Msa0598720:exon
TATTTGATGGACTAAATTGA+CGG 0.491712 4_2:+36135123 None:intergenic
AGGGCATGGAGAGGTTCTAC+TGG 0.510423 4_2:-36133828 Msa0598720:CDS
AAATGCTTATAGAATGTACT+TGG 0.517363 4_2:-36133660 Msa0598720:CDS
CAATGGAGGAGGCAAGGGCA+TGG 0.517572 4_2:-36133842 Msa0598720:CDS
TTGATTCTATTTCTCTGCAA+CGG 0.519902 4_2:+36135063 None:intergenic
TCTTGTACAGATTCAGCGCA+GGG 0.523854 4_2:+36133735 None:intergenic
AAGGAGCAATGGAGGAGGCA+AGG 0.528602 4_2:-36133848 Msa0598720:CDS
ACATGACATAACCTGCAAGT+TGG 0.533996 4_2:+36134835 None:intergenic
GGAGTTGGAACCTGCGAGCT+TGG 0.538376 4_2:+36134779 None:intergenic
GCAGAACAAGAAAAGTTTCG+CGG 0.541873 4_2:-36133787 Msa0598720:CDS
TGGAGAGGTTCTACTGGAAG+CGG 0.545320 4_2:-36133822 Msa0598720:CDS
GCTTCTATCACCAAGCTCGC+AGG 0.549548 4_2:-36134789 Msa0598720:intron
TTCTTGTACAGATTCAGCGC+AGG 0.552919 4_2:+36133734 None:intergenic
TGCTTCACGTCGATCCGTTG+AGG 0.562820 4_2:-36133760 Msa0598720:CDS
ATAGAAGCATACAATGAAGA+AGG 0.562971 4_2:+36134804 None:intergenic
TCACGCTCACGCACGAGTGA+TGG 0.564291 4_2:-36135021 Msa0598720:exon
AGGAGCAATGGAGGAGGCAA+GGG 0.571699 4_2:-36133847 Msa0598720:CDS
CAGAAACAGTGGTAAAGTAG+TGG 0.572015 4_2:-36134973 Msa0598720:CDS
CTTCTGTAGTGAAGGAGCAA+TGG 0.596774 4_2:-36133859 Msa0598720:intron
CTGAATCTGTACAAGAAGCA+TGG 0.599242 4_2:-36133728 Msa0598720:CDS
CTGTAGTGAAGGAGCAATGG+AGG 0.602271 4_2:-36133856 Msa0598720:intron
TAGTGAAGGAGCAATGGAGG+AGG 0.603189 4_2:-36133853 Msa0598720:CDS
GGAACATTGTGTTTGTAACA+TGG 0.606394 4_2:-36133560 Msa0598720:three_prime_UTR
CGCACGAGTGATGGCAACTG+TGG 0.609912 4_2:-36135012 Msa0598720:exon
TTCAGCGCAGGGGTCCTCAA+CGG 0.614591 4_2:+36133746 None:intergenic
GGAACATTGTGTTTGTGACA+TGG 0.627922 4_2:-36133480 Msa0598720:three_prime_UTR
TTAGAACTTGACAGAAACAG+TGG 0.638839 4_2:-36134984 Msa0598720:CDS
GAGGAGGCAAGGGCATGGAG+AGG 0.640974 4_2:-36133837 Msa0598720:CDS
CTTGTACAGATTCAGCGCAG+GGG 0.671497 4_2:+36133736 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAACTATCTTTAATATA+AGG - chr4_2:36134599-36134618 Msa0598720:intron 10.0%
!! ATTAACTATCTTTAATATAA+GGG - chr4_2:36134600-36134619 Msa0598720:intron 10.0%
!! AATTAACTATCTTTAATATA+AGG - chr4_2:36134599-36134618 Msa0598720:intron 10.0%
!! ATTAACTATCTTTAATATAA+GGG - chr4_2:36134600-36134619 Msa0598720:intron 10.0%
!!! ATAGATTTTCATATATTTGA+TGG + chr4_2:36133490-36133509 None:intergenic 15.0%
!! AAAGAAATTATCCAATTTAT+AGG - chr4_2:36134211-36134230 Msa0598720:intron 15.0%
!! AAGAAATTATCCAATTTATA+GGG - chr4_2:36134212-36134231 Msa0598720:intron 15.0%
!!! ATAGATTTTCATATATTTGA+TGG + chr4_2:36133490-36133509 None:intergenic 15.0%
!! AAAGAAATTATCCAATTTAT+AGG - chr4_2:36134211-36134230 Msa0598720:intron 15.0%
!! AAGAAATTATCCAATTTATA+GGG - chr4_2:36134212-36134231 Msa0598720:intron 15.0%
!! AGAAATTATCCAATTTATAG+GGG - chr4_2:36134213-36134232 Msa0598720:intron 20.0%
!! TTATATTAACCCCTATAAAT+TGG + chr4_2:36134225-36134244 None:intergenic 20.0%
!! TTAAACAAACACAATATTAC+AGG + chr4_2:36135080-36135099 None:intergenic 20.0%
!! AGAAATTATCCAATTTATAG+GGG - chr4_2:36134213-36134232 Msa0598720:intron 20.0%
!! TTATATTAACCCCTATAAAT+TGG + chr4_2:36134225-36134244 None:intergenic 20.0%
!! TTAAACAAACACAATATTAC+AGG + chr4_2:36135080-36135099 None:intergenic 20.0%
!!! TATTTGATGGACTAAATTGA+CGG + chr4_2:36133477-36133496 None:intergenic 25.0%
!!! TTTGAGTTCATTTTCAAAGA+AGG + chr4_2:36133704-36133723 None:intergenic 25.0%
!!! CAATTTTTCAAACAGCTTAT+AGG + chr4_2:36133870-36133889 None:intergenic 25.0%
! CCAGAATTATTATAAAACCT+GGG - chr4_2:36134120-36134139 Msa0598720:intron 25.0%
! AAAATTTGCAACCCATTTAT+AGG - chr4_2:36134148-36134167 Msa0598720:intron 25.0%
! AAATTTGCAACCCATTTATA+GGG - chr4_2:36134149-36134168 Msa0598720:intron 25.0%
! TTATGTAAACCCCTATAAAT+GGG + chr4_2:36134162-36134181 None:intergenic 25.0%
! CTAAACTATTGAAAGATAGT+AGG + chr4_2:36134395-36134414 None:intergenic 25.0%
! ATACAAGCATTTCATTAGTT+GGG + chr4_2:36134657-36134676 None:intergenic 25.0%
! ACAATATGGGTATGTAATAT+GGG - chr4_2:36134704-36134723 Msa0598720:intron 25.0%
! AAATGCTTATAGAATGTACT+TGG - chr4_2:36134937-36134956 Msa0598720:CDS 25.0%
!! CACTTTTATATACGTTATCA+TGG - chr4_2:36134994-36135013 Msa0598720:CDS 25.0%
! GTCAATTCAAAAAACAGATT+TGG - chr4_2:36135016-36135035 Msa0598720:exon 25.0%
! TTAAGCACTAAAAACAAATC+TGG - chr4_2:36135096-36135115 Msa0598720:five_prime_UTR 25.0%
!!! TATTTGATGGACTAAATTGA+CGG + chr4_2:36133477-36133496 None:intergenic 25.0%
!!! TTTGAGTTCATTTTCAAAGA+AGG + chr4_2:36133704-36133723 None:intergenic 25.0%
!!! CAATTTTTCAAACAGCTTAT+AGG + chr4_2:36133870-36133889 None:intergenic 25.0%
! CCAGAATTATTATAAAACCT+GGG - chr4_2:36134120-36134139 Msa0598720:intron 25.0%
! AAAATTTGCAACCCATTTAT+AGG - chr4_2:36134148-36134167 Msa0598720:intron 25.0%
! AAATTTGCAACCCATTTATA+GGG - chr4_2:36134149-36134168 Msa0598720:intron 25.0%
! TTATGTAAACCCCTATAAAT+GGG + chr4_2:36134162-36134181 None:intergenic 25.0%
! CTAAACTATTGAAAGATAGT+AGG + chr4_2:36134395-36134414 None:intergenic 25.0%
! ATACAAGCATTTCATTAGTT+GGG + chr4_2:36134657-36134676 None:intergenic 25.0%
! ACAATATGGGTATGTAATAT+GGG - chr4_2:36134704-36134723 Msa0598720:intron 25.0%
! AAATGCTTATAGAATGTACT+TGG - chr4_2:36134937-36134956 Msa0598720:CDS 25.0%
!! CACTTTTATATACGTTATCA+TGG - chr4_2:36134994-36135013 Msa0598720:CDS 25.0%
! GTCAATTCAAAAAACAGATT+TGG - chr4_2:36135016-36135035 Msa0598720:exon 25.0%
! TTAAGCACTAAAAACAAATC+TGG - chr4_2:36135096-36135115 Msa0598720:five_prime_UTR 25.0%
! TTGATTCTATTTCTCTGCAA+CGG + chr4_2:36133537-36133556 None:intergenic 30.0%
! ATAGAAGCATACAATGAAGA+AGG + chr4_2:36133796-36133815 None:intergenic 30.0%
GCCAGAATTATTATAAAACC+TGG - chr4_2:36134119-36134138 Msa0598720:intron 30.0%
!! CCCAGGTTTTATAATAATTC+TGG + chr4_2:36134123-36134142 None:intergenic 30.0%
AATTTGCAACCCATTTATAG+GGG - chr4_2:36134150-36134169 Msa0598720:intron 30.0%
CTTATGTAAACCCCTATAAA+TGG + chr4_2:36134163-36134182 None:intergenic 30.0%
AAATTGCAAAAACATTCAGC+CGG - chr4_2:36134269-36134288 Msa0598720:intron 30.0%
!! ACTAAGTTGATGTATTAAGG+GGG + chr4_2:36134355-36134374 None:intergenic 30.0%
!! GACTAAGTTGATGTATTAAG+GGG + chr4_2:36134356-36134375 None:intergenic 30.0%
!! CGACTAAGTTGATGTATTAA+GGG + chr4_2:36134357-36134376 None:intergenic 30.0%
!! ACGACTAAGTTGATGTATTA+AGG + chr4_2:36134358-36134377 None:intergenic 30.0%
TAATACATCAACTTAGTCGT+TGG - chr4_2:36134358-36134377 Msa0598720:intron 30.0%
CATACAAGCATTTCATTAGT+TGG + chr4_2:36134658-36134677 None:intergenic 30.0%
! TTTGTTGGTTACTCACAATA+TGG - chr4_2:36134690-36134709 Msa0598720:intron 30.0%
! TTGTTGGTTACTCACAATAT+GGG - chr4_2:36134691-36134710 Msa0598720:intron 30.0%
CACAATATGGGTATGTAATA+TGG - chr4_2:36134703-36134722 Msa0598720:intron 30.0%
! GTATGTAATATGGGATGTTT+TGG - chr4_2:36134713-36134732 Msa0598720:intron 30.0%
! TTGATTCTATTTCTCTGCAA+CGG + chr4_2:36133537-36133556 None:intergenic 30.0%
! ATAGAAGCATACAATGAAGA+AGG + chr4_2:36133796-36133815 None:intergenic 30.0%
GCCAGAATTATTATAAAACC+TGG - chr4_2:36134119-36134138 Msa0598720:intron 30.0%
!! CCCAGGTTTTATAATAATTC+TGG + chr4_2:36134123-36134142 None:intergenic 30.0%
AATTTGCAACCCATTTATAG+GGG - chr4_2:36134150-36134169 Msa0598720:intron 30.0%
CTTATGTAAACCCCTATAAA+TGG + chr4_2:36134163-36134182 None:intergenic 30.0%
AAATTGCAAAAACATTCAGC+CGG - chr4_2:36134269-36134288 Msa0598720:intron 30.0%
!! ACTAAGTTGATGTATTAAGG+GGG + chr4_2:36134355-36134374 None:intergenic 30.0%
!! GACTAAGTTGATGTATTAAG+GGG + chr4_2:36134356-36134375 None:intergenic 30.0%
!! CGACTAAGTTGATGTATTAA+GGG + chr4_2:36134357-36134376 None:intergenic 30.0%
!! ACGACTAAGTTGATGTATTA+AGG + chr4_2:36134358-36134377 None:intergenic 30.0%
TAATACATCAACTTAGTCGT+TGG - chr4_2:36134358-36134377 Msa0598720:intron 30.0%
CATACAAGCATTTCATTAGT+TGG + chr4_2:36134658-36134677 None:intergenic 30.0%
! TTTGTTGGTTACTCACAATA+TGG - chr4_2:36134690-36134709 Msa0598720:intron 30.0%
! TTGTTGGTTACTCACAATAT+GGG - chr4_2:36134691-36134710 Msa0598720:intron 30.0%
CACAATATGGGTATGTAATA+TGG - chr4_2:36134703-36134722 Msa0598720:intron 30.0%
! GTATGTAATATGGGATGTTT+TGG - chr4_2:36134713-36134732 Msa0598720:intron 30.0%
TGCAACGGTGACAATATTTA+GGG + chr4_2:36133522-36133541 None:intergenic 35.0%
TTAGAACTTGACAGAAACAG+TGG - chr4_2:36133613-36133632 Msa0598720:three_prime_UTR 35.0%
AGTGGAAGAGATAGTGAAAT+TGG - chr4_2:36133642-36133661 Msa0598720:exon 35.0%
GAACTCAAAAAGAAGAACAG+TGG - chr4_2:36133715-36133734 Msa0598720:CDS 35.0%
CAGGTTATGTCATGTATTCT+TGG - chr4_2:36133770-36133789 Msa0598720:CDS 35.0%
AGCATAAAAAAGAGTGGAGT+TGG + chr4_2:36133836-36133855 None:intergenic 35.0%
CTTGACAGCATAAAAAAGAG+TGG + chr4_2:36133842-36133861 None:intergenic 35.0%
! TTTTGTAAGTGTGACTCAGT+TGG - chr4_2:36134032-36134051 Msa0598720:intron 35.0%
!!! GTTGCAAATTTTTTTTGCCC+AGG + chr4_2:36134140-36134159 None:intergenic 35.0%
TGATTTGAGCAAAAGGCTAA+AGG + chr4_2:36134322-36134341 None:intergenic 35.0%
!! ACTCAACTGATTTGAGCAAA+AGG + chr4_2:36134329-36134348 None:intergenic 35.0%
TTGTGTGTGTGAATTTCATG+TGG + chr4_2:36134509-36134528 None:intergenic 35.0%
CATTGTCGATATAGTTAGGT+TGG + chr4_2:36134566-36134585 None:intergenic 35.0%
CTGACATTGTCGATATAGTT+AGG + chr4_2:36134570-36134589 None:intergenic 35.0%
CTATATCGACAATGTCAGTT+AGG - chr4_2:36134572-36134591 Msa0598720:intron 35.0%
!! ATGCGAATCCTTTGATTTGT+TGG - chr4_2:36134675-36134694 Msa0598720:intron 35.0%
GTGAGTAACCAACAAATCAA+AGG + chr4_2:36134686-36134705 None:intergenic 35.0%
!!! TTTTGGTTCTTCTGTAGTGA+AGG - chr4_2:36134730-36134749 Msa0598720:intron 35.0%
TGGACTTCGATTCAAATTGA+AGG - chr4_2:36134957-36134976 Msa0598720:CDS 35.0%
GGAACATTGTGTTTGTAACA+TGG - chr4_2:36135037-36135056 Msa0598720:five_prime_UTR 35.0%
TGCAACGGTGACAATATTTA+GGG + chr4_2:36133522-36133541 None:intergenic 35.0%
TTAGAACTTGACAGAAACAG+TGG - chr4_2:36133613-36133632 Msa0598720:three_prime_UTR 35.0%
AGTGGAAGAGATAGTGAAAT+TGG - chr4_2:36133642-36133661 Msa0598720:exon 35.0%
GAACTCAAAAAGAAGAACAG+TGG - chr4_2:36133715-36133734 Msa0598720:CDS 35.0%
CAGGTTATGTCATGTATTCT+TGG - chr4_2:36133770-36133789 Msa0598720:CDS 35.0%
AGCATAAAAAAGAGTGGAGT+TGG + chr4_2:36133836-36133855 None:intergenic 35.0%
CTTGACAGCATAAAAAAGAG+TGG + chr4_2:36133842-36133861 None:intergenic 35.0%
! TTTTGTAAGTGTGACTCAGT+TGG - chr4_2:36134032-36134051 Msa0598720:intron 35.0%
!!! GTTGCAAATTTTTTTTGCCC+AGG + chr4_2:36134140-36134159 None:intergenic 35.0%
TGATTTGAGCAAAAGGCTAA+AGG + chr4_2:36134322-36134341 None:intergenic 35.0%
!! ACTCAACTGATTTGAGCAAA+AGG + chr4_2:36134329-36134348 None:intergenic 35.0%
TTGTGTGTGTGAATTTCATG+TGG + chr4_2:36134509-36134528 None:intergenic 35.0%
CATTGTCGATATAGTTAGGT+TGG + chr4_2:36134566-36134585 None:intergenic 35.0%
CTGACATTGTCGATATAGTT+AGG + chr4_2:36134570-36134589 None:intergenic 35.0%
CTATATCGACAATGTCAGTT+AGG - chr4_2:36134572-36134591 Msa0598720:intron 35.0%
!! ATGCGAATCCTTTGATTTGT+TGG - chr4_2:36134675-36134694 Msa0598720:intron 35.0%
GTGAGTAACCAACAAATCAA+AGG + chr4_2:36134686-36134705 None:intergenic 35.0%
!!! TTTTGGTTCTTCTGTAGTGA+AGG - chr4_2:36134730-36134749 Msa0598720:intron 35.0%
TGGACTTCGATTCAAATTGA+AGG - chr4_2:36134957-36134976 Msa0598720:CDS 35.0%
GGAACATTGTGTTTGTAACA+TGG - chr4_2:36135037-36135056 Msa0598720:five_prime_UTR 35.0%
CTGCAACGGTGACAATATTT+AGG + chr4_2:36133523-36133542 None:intergenic 40.0%
CAGAAACAGTGGTAAAGTAG+TGG - chr4_2:36133624-36133643 Msa0598720:exon 40.0%
CATGACATAACCTGCAAGTT+GGG + chr4_2:36133764-36133783 None:intergenic 40.0%
ACATGACATAACCTGCAAGT+TGG + chr4_2:36133765-36133784 None:intergenic 40.0%
!! CAGTTGGTAAGCTTGGATAT+TGG - chr4_2:36134048-36134067 Msa0598720:intron 40.0%
AAGCTTGGATATTGGTATGC+AGG - chr4_2:36134056-36134075 Msa0598720:intron 40.0%
AGCTTGGATATTGGTATGCA+GGG - chr4_2:36134057-36134076 Msa0598720:intron 40.0%
TGAGCAAAAGGCTAAAGGTA+GGG + chr4_2:36134317-36134336 None:intergenic 40.0%
TTGAGCAAAAGGCTAAAGGT+AGG + chr4_2:36134318-36134337 None:intergenic 40.0%
AATTCACACACACAACTGCA+AGG - chr4_2:36134514-36134533 Msa0598720:intron 40.0%
GCAGAACAAGAAAAGTTTCG+CGG - chr4_2:36134810-36134829 Msa0598720:CDS 40.0%
CTGAATCTGTACAAGAAGCA+TGG - chr4_2:36134869-36134888 Msa0598720:CDS 40.0%
! TGGCTTTCATGTTGACTGTT+TGG - chr4_2:36134889-36134908 Msa0598720:CDS 40.0%
! CATGTTGACTGTTTGGTTGA+AGG - chr4_2:36134896-36134915 Msa0598720:CDS 40.0%
GGAACATTGTGTTTGTGACA+TGG - chr4_2:36135117-36135136 Msa0598720:five_prime_UTR 40.0%
CTGCAACGGTGACAATATTT+AGG + chr4_2:36133523-36133542 None:intergenic 40.0%
CAGAAACAGTGGTAAAGTAG+TGG - chr4_2:36133624-36133643 Msa0598720:exon 40.0%
CATGACATAACCTGCAAGTT+GGG + chr4_2:36133764-36133783 None:intergenic 40.0%
ACATGACATAACCTGCAAGT+TGG + chr4_2:36133765-36133784 None:intergenic 40.0%
!! CAGTTGGTAAGCTTGGATAT+TGG - chr4_2:36134048-36134067 Msa0598720:intron 40.0%
AAGCTTGGATATTGGTATGC+AGG - chr4_2:36134056-36134075 Msa0598720:intron 40.0%
AGCTTGGATATTGGTATGCA+GGG - chr4_2:36134057-36134076 Msa0598720:intron 40.0%
TGAGCAAAAGGCTAAAGGTA+GGG + chr4_2:36134317-36134336 None:intergenic 40.0%
TTGAGCAAAAGGCTAAAGGT+AGG + chr4_2:36134318-36134337 None:intergenic 40.0%
AATTCACACACACAACTGCA+AGG - chr4_2:36134514-36134533 Msa0598720:intron 40.0%
GCAGAACAAGAAAAGTTTCG+CGG - chr4_2:36134810-36134829 Msa0598720:CDS 40.0%
CTGAATCTGTACAAGAAGCA+TGG - chr4_2:36134869-36134888 Msa0598720:CDS 40.0%
! TGGCTTTCATGTTGACTGTT+TGG - chr4_2:36134889-36134908 Msa0598720:CDS 40.0%
! CATGTTGACTGTTTGGTTGA+AGG - chr4_2:36134896-36134915 Msa0598720:CDS 40.0%
GGAACATTGTGTTTGTGACA+TGG - chr4_2:36135117-36135136 Msa0598720:five_prime_UTR 40.0%
GAAGCGAGTGATTCGTGTTT+GGG + chr4_2:36133682-36133701 None:intergenic 45.0%
!! TGTGACTCAGTTGGTAAGCT+TGG - chr4_2:36134041-36134060 Msa0598720:intron 45.0%
!! AATAATTCTGGCTCTGCCAC+TGG + chr4_2:36134111-36134130 None:intergenic 45.0%
GAGCAAAAGGCTAAAGGTAG+GGG + chr4_2:36134316-36134335 None:intergenic 45.0%
ACACACAACTGCAAGGTCTT+GGG - chr4_2:36134521-36134540 Msa0598720:intron 45.0%
CTTCTGTAGTGAAGGAGCAA+TGG - chr4_2:36134738-36134757 Msa0598720:intron 45.0%
TCTTGTACAGATTCAGCGCA+GGG + chr4_2:36134865-36134884 None:intergenic 45.0%
TTCTTGTACAGATTCAGCGC+AGG + chr4_2:36134866-36134885 None:intergenic 45.0%
GAAGCGAGTGATTCGTGTTT+GGG + chr4_2:36133682-36133701 None:intergenic 45.0%
!! TGTGACTCAGTTGGTAAGCT+TGG - chr4_2:36134041-36134060 Msa0598720:intron 45.0%
!! AATAATTCTGGCTCTGCCAC+TGG + chr4_2:36134111-36134130 None:intergenic 45.0%
GAGCAAAAGGCTAAAGGTAG+GGG + chr4_2:36134316-36134335 None:intergenic 45.0%
ACACACAACTGCAAGGTCTT+GGG - chr4_2:36134521-36134540 Msa0598720:intron 45.0%
CTTCTGTAGTGAAGGAGCAA+TGG - chr4_2:36134738-36134757 Msa0598720:intron 45.0%
TCTTGTACAGATTCAGCGCA+GGG + chr4_2:36134865-36134884 None:intergenic 45.0%
TTCTTGTACAGATTCAGCGC+AGG + chr4_2:36134866-36134885 None:intergenic 45.0%
GGAAGCGAGTGATTCGTGTT+TGG + chr4_2:36133683-36133702 None:intergenic 50.0%
GGTATGCAGGGTTCGAGATT+CGG - chr4_2:36134069-36134088 Msa0598720:intron 50.0%
GTATGCAGGGTTCGAGATTC+GGG - chr4_2:36134070-36134089 Msa0598720:intron 50.0%
TATGCAGGGTTCGAGATTCG+GGG - chr4_2:36134071-36134090 Msa0598720:intron 50.0%
GGTTCGAACCATTTAGCCAG+TGG - chr4_2:36134092-36134111 Msa0598720:intron 50.0%
TAAAGGTAGGGGCGGTTCTA+AGG + chr4_2:36134305-36134324 None:intergenic 50.0%
CAAAAGGCTAAAGGTAGGGG+CGG + chr4_2:36134313-36134332 None:intergenic 50.0%
CACACACAACTGCAAGGTCT+TGG - chr4_2:36134520-36134539 Msa0598720:intron 50.0%
CTGTAGTGAAGGAGCAATGG+AGG - chr4_2:36134741-36134760 Msa0598720:intron 50.0%
TAGTGAAGGAGCAATGGAGG+AGG - chr4_2:36134744-36134763 Msa0598720:intron 50.0%
TGGAGAGGTTCTACTGGAAG+CGG - chr4_2:36134775-36134794 Msa0598720:intron 50.0%
CTTGTACAGATTCAGCGCAG+GGG + chr4_2:36134864-36134883 None:intergenic 50.0%
GGAAGCGAGTGATTCGTGTT+TGG + chr4_2:36133683-36133702 None:intergenic 50.0%
GGTATGCAGGGTTCGAGATT+CGG - chr4_2:36134069-36134088 Msa0598720:intron 50.0%
GTATGCAGGGTTCGAGATTC+GGG - chr4_2:36134070-36134089 Msa0598720:intron 50.0%
TATGCAGGGTTCGAGATTCG+GGG - chr4_2:36134071-36134090 Msa0598720:intron 50.0%
GGTTCGAACCATTTAGCCAG+TGG - chr4_2:36134092-36134111 Msa0598720:intron 50.0%
TAAAGGTAGGGGCGGTTCTA+AGG + chr4_2:36134305-36134324 None:intergenic 50.0%
CAAAAGGCTAAAGGTAGGGG+CGG + chr4_2:36134313-36134332 None:intergenic 50.0%
CACACACAACTGCAAGGTCT+TGG - chr4_2:36134520-36134539 Msa0598720:intron 50.0%
CTGTAGTGAAGGAGCAATGG+AGG - chr4_2:36134741-36134760 Msa0598720:intron 50.0%
TAGTGAAGGAGCAATGGAGG+AGG - chr4_2:36134744-36134763 Msa0598720:intron 50.0%
TGGAGAGGTTCTACTGGAAG+CGG - chr4_2:36134775-36134794 Msa0598720:intron 50.0%
CTTGTACAGATTCAGCGCAG+GGG + chr4_2:36134864-36134883 None:intergenic 50.0%
CACGTTGATGCCCAACTTGC+AGG - chr4_2:36133751-36133770 Msa0598720:CDS 55.0%
GCTTCTATCACCAAGCTCGC+AGG - chr4_2:36133808-36133827 Msa0598720:CDS 55.0%
! TGGCTCTGCCACTGGCTAAA+TGG + chr4_2:36134103-36134122 None:intergenic 55.0%
AAAACATTCAGCCGGAGCCC+AGG - chr4_2:36134277-36134296 Msa0598720:intron 55.0%
AAACATTCAGCCGGAGCCCA+GGG - chr4_2:36134278-36134297 Msa0598720:intron 55.0%
AAGGAGCAATGGAGGAGGCA+AGG - chr4_2:36134749-36134768 Msa0598720:intron 55.0%
AGGAGCAATGGAGGAGGCAA+GGG - chr4_2:36134750-36134769 Msa0598720:intron 55.0%
AGGGCATGGAGAGGTTCTAC+TGG - chr4_2:36134769-36134788 Msa0598720:intron 55.0%
TGCTTCACGTCGATCCGTTG+AGG - chr4_2:36134837-36134856 Msa0598720:CDS 55.0%
CACGTTGATGCCCAACTTGC+AGG - chr4_2:36133751-36133770 Msa0598720:CDS 55.0%
GCTTCTATCACCAAGCTCGC+AGG - chr4_2:36133808-36133827 Msa0598720:CDS 55.0%
! TGGCTCTGCCACTGGCTAAA+TGG + chr4_2:36134103-36134122 None:intergenic 55.0%
AAAACATTCAGCCGGAGCCC+AGG - chr4_2:36134277-36134296 Msa0598720:intron 55.0%
AAACATTCAGCCGGAGCCCA+GGG - chr4_2:36134278-36134297 Msa0598720:intron 55.0%
AAGGAGCAATGGAGGAGGCA+AGG - chr4_2:36134749-36134768 Msa0598720:intron 55.0%
AGGAGCAATGGAGGAGGCAA+GGG - chr4_2:36134750-36134769 Msa0598720:intron 55.0%
AGGGCATGGAGAGGTTCTAC+TGG - chr4_2:36134769-36134788 Msa0598720:intron 55.0%
TGCTTCACGTCGATCCGTTG+AGG - chr4_2:36134837-36134856 Msa0598720:CDS 55.0%
TCACGCTCACGCACGAGTGA+TGG - chr4_2:36133576-36133595 Msa0598720:three_prime_UTR 60.0%
CGCACGAGTGATGGCAACTG+TGG - chr4_2:36133585-36133604 Msa0598720:three_prime_UTR 60.0%
GGAGTTGGAACCTGCGAGCT+TGG + chr4_2:36133821-36133840 None:intergenic 60.0%
AACATTCAGCCGGAGCCCAG+GGG - chr4_2:36134279-36134298 Msa0598720:intron 60.0%
CAATGGAGGAGGCAAGGGCA+TGG - chr4_2:36134755-36134774 Msa0598720:intron 60.0%
TTCAGCGCAGGGGTCCTCAA+CGG + chr4_2:36134854-36134873 None:intergenic 60.0%
TCACGCTCACGCACGAGTGA+TGG - chr4_2:36133576-36133595 Msa0598720:three_prime_UTR 60.0%
CGCACGAGTGATGGCAACTG+TGG - chr4_2:36133585-36133604 Msa0598720:three_prime_UTR 60.0%
GGAGTTGGAACCTGCGAGCT+TGG + chr4_2:36133821-36133840 None:intergenic 60.0%
AACATTCAGCCGGAGCCCAG+GGG - chr4_2:36134279-36134298 Msa0598720:intron 60.0%
CAATGGAGGAGGCAAGGGCA+TGG - chr4_2:36134755-36134774 Msa0598720:intron 60.0%
TTCAGCGCAGGGGTCCTCAA+CGG + chr4_2:36134854-36134873 None:intergenic 60.0%
!! GTTCTAAGGCCCCTGGGCTC+CGG + chr4_2:36134291-36134310 None:intergenic 65.0%
! GAGGAGGCAAGGGCATGGAG+AGG - chr4_2:36134760-36134779 Msa0598720:intron 65.0%
!! GTTCTAAGGCCCCTGGGCTC+CGG + chr4_2:36134291-36134310 None:intergenic 65.0%
! GAGGAGGCAAGGGCATGGAG+AGG - chr4_2:36134760-36134779 Msa0598720:intron 65.0%
!! GGGGCGGTTCTAAGGCCCCT+GGG + chr4_2:36134297-36134316 None:intergenic 70.0%
!! AGGGGCGGTTCTAAGGCCCC+TGG + chr4_2:36134298-36134317 None:intergenic 70.0%
!! GGGGCGGTTCTAAGGCCCCT+GGG + chr4_2:36134297-36134316 None:intergenic 70.0%
!! AGGGGCGGTTCTAAGGCCCC+TGG + chr4_2:36134298-36134317 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 36133459 36135160 36133459 ID=Msa0598720;Name=Msa0598720
chr4_2 mRNA 36133459 36135160 36133459 ID=Msa0598720-mRNA-1;Parent=Msa0598720;Name=Msa0598720-mRNA-1;_AED=0.04;_eAED=0.04;_QI=136|1|1|1|1|1|2|184|154
chr4_2 exon 36133459 36133872 36133459 ID=Msa0598720-mRNA-1:exon:8250;Parent=Msa0598720-mRNA-1
chr4_2 exon 36134790 36135160 36134790 ID=Msa0598720-mRNA-1:exon:8249;Parent=Msa0598720-mRNA-1
chr4_2 five_prime_UTR 36135025 36135160 36135025 ID=Msa0598720-mRNA-1:five_prime_utr;Parent=Msa0598720-mRNA-1
chr4_2 CDS 36134790 36135024 36134790 ID=Msa0598720-mRNA-1:cds;Parent=Msa0598720-mRNA-1
chr4_2 CDS 36133643 36133872 36133643 ID=Msa0598720-mRNA-1:cds;Parent=Msa0598720-mRNA-1
chr4_2 three_prime_UTR 36133459 36133642 36133459 ID=Msa0598720-mRNA-1:three_prime_utr;Parent=Msa0598720-mRNA-1
Gene Sequence

>Msa0598720

ATGGCAACTGTGGAGATTTTAGAACTTGACAGAAACAGTGGTAAAGTAGTGGAAGAGATAGTGAAATTGGAGAGAAAGATATTTCCCAAACACGAATCACTCGCTTCCTTCTTTGAAAATGAACTCAAAAAGAAGAACAGTGGATTGCTTTATCTTCACGTTGATGCCCAACTTGCAGGTTATGTCATGTATTCTTGGCCTTCTTCATTGTATGCTTCTATCACCAAGCTCGCAGTGAAGGAGCAATGGAGGAGGCAAGGGCATGGAGAGGTTCTACTGGAAGCGGCAATCGAGAAATGCAGAACAAGAAAAGTTTCGCGGATAATGCTTCACGTCGATCCGTTGAGGACCCCTGCGCTGAATCTGTACAAGAAGCATGGCTTTCATGTTGACTGTTTGGTTGAAGGCTATTACTCTTCTGATAGAAATGCTTATAGAATGTACTTGGACTTCGATTCAAATTGA
Protein sequence

>Msa0598720

MATVEILELDRNSGKVVEEIVKLERKIFPKHESLASFFENELKKKNSGLLYLHVDAQLAGYVMYSWPSSLYASITKLAVKEQWRRQGHGEVLLEAAIEKCRTRKVSRIMLHVDPLRTPALNLYKKHGFHVDCLVEGYYSSDRNAYRMYLDFDSN*