Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0510350 | KEH34975.1 | 97.137 | 489 | 14 | 0 | 1 | 489 | 1 | 489 | 0.0 | 977 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0510350 | sp|F4JF21|PTA10_ARATH | 70.722 | 485 | 129 | 4 | 1 | 478 | 1 | 479 | 0.0 | 705 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0510350 | A0A072V9X0 | 97.137 | 489 | 14 | 0 | 1 | 489 | 1 | 489 | 0.0 | 977 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003640 | Msa0510350 | 0.818831 | 1.596727e-52 | -8.615850e-47 |
| Msa0003780 | Msa0510350 | 0.829405 | 5.242602e-55 | -8.615850e-47 |
| Msa0004860 | Msa0510350 | 0.815587 | 8.564595e-52 | -8.615850e-47 |
| Msa0004920 | Msa0510350 | 0.802871 | 4.566922e-49 | -8.615850e-47 |
| Msa0004980 | Msa0510350 | 0.804783 | 1.829998e-49 | -8.615850e-47 |
| Msa0011960 | Msa0510350 | 0.827386 | 1.610080e-54 | -8.615850e-47 |
| Msa0012240 | Msa0510350 | 0.834968 | 2.205065e-56 | -8.615850e-47 |
| Msa0013050 | Msa0510350 | -0.837326 | 5.552681e-57 | -8.615850e-47 |
| Msa0025730 | Msa0510350 | 0.828856 | 7.122161e-55 | -8.615850e-47 |
| Msa0027260 | Msa0510350 | 0.806680 | 7.314498e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0510350 | MtrunA17_Chr3g0116321 | 98.954 | 478 | 5 | 0 | 1 | 478 | 1 | 478 | 0.0 | 976 |
| Msa0510350 | MtrunA17_Chr3g0116421 | 98.502 | 267 | 4 | 0 | 478 | 744 | 1644 | 1910 | 1.94e-172 | 541 |
| Msa0510350 | MtrunA17_Chr1g0213461 | 66.171 | 269 | 91 | 0 | 473 | 741 | 1626 | 1894 | 1.41e-108 | 363 |
| Msa0510350 | MtrunA17_Chr3g0130141 | 52.652 | 264 | 122 | 2 | 481 | 741 | 1655 | 1918 | 1.89e-86 | 299 |
| Msa0510350 | MtrunA17_Chr8g0384221 | 50.379 | 264 | 128 | 2 | 481 | 741 | 329 | 592 | 3.08e-86 | 284 |
| Msa0510350 | MtrunA17_Chr1g0203941 | 47.266 | 256 | 132 | 2 | 492 | 744 | 2 | 257 | 4.11e-82 | 262 |
| Msa0510350 | MtrunA17_Chr7g0214481 | 49.621 | 264 | 129 | 3 | 480 | 739 | 1686 | 1949 | 9.24e-82 | 285 |
| Msa0510350 | MtrunA17_Chr3g0096261 | 47.253 | 273 | 141 | 2 | 475 | 744 | 1635 | 1907 | 1.40e-79 | 279 |
| Msa0510350 | MtrunA17_Chr2g0308711 | 47.909 | 263 | 134 | 2 | 480 | 739 | 1676 | 1938 | 1.89e-79 | 278 |
| Msa0510350 | MtrunA17_Chr1g0213421 | 49.049 | 263 | 131 | 2 | 480 | 739 | 1684 | 1946 | 5.91e-73 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0510350 | AT3G48500.1 | 70.722 | 485 | 129 | 4 | 1 | 478 | 1 | 479 | 0.0 | 705 |
| Msa0510350 | AT3G48500.2 | 66.732 | 514 | 129 | 5 | 1 | 478 | 1 | 508 | 0.0 | 690 |
| Msa0510350 | AT2G36850.1 | 75.000 | 272 | 68 | 0 | 473 | 744 | 1633 | 1904 | 1.22e-129 | 424 |
| Msa0510350 | AT3G07160.3 | 62.825 | 269 | 100 | 0 | 473 | 741 | 1621 | 1889 | 1.06e-107 | 362 |
| Msa0510350 | AT3G07160.2 | 62.825 | 269 | 100 | 0 | 473 | 741 | 1621 | 1889 | 1.06e-107 | 362 |
| Msa0510350 | AT3G07160.1 | 62.825 | 269 | 100 | 0 | 473 | 741 | 1619 | 1887 | 1.10e-107 | 362 |
| Msa0510350 | AT1G06490.2 | 51.538 | 260 | 123 | 2 | 483 | 739 | 1666 | 1925 | 6.91e-86 | 298 |
| Msa0510350 | AT1G06490.1 | 50.951 | 263 | 126 | 2 | 480 | 739 | 1663 | 1925 | 8.72e-86 | 297 |
| Msa0510350 | AT1G05570.2 | 49.624 | 266 | 130 | 3 | 480 | 741 | 1637 | 1902 | 2.63e-80 | 281 |
| Msa0510350 | AT1G05570.4 | 49.624 | 266 | 130 | 3 | 480 | 741 | 1678 | 1943 | 2.68e-80 | 281 |
Find 213 sgRNAs with CRISPR-Local
Find 1038 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGTTGGGAGTCTTGG+TGG | 0.524891 | 3_4:-59372069 | Msa0510350:CDS |
| AAAGGAGGAGTAGGAGTGAA+AGG | 0.423758 | 3_4:-59372094 | Msa0510350:CDS |
| AAAGTGAAGAGATAAGAATT+GGG | 0.395883 | 3_4:+59382844 | None:intergenic |
| AACCAATCATTGTTCTTTAT+AGG | 0.253075 | 3_4:+59380460 | None:intergenic |
| AACCGCGTTCGATCCAGTTG+CGG | 0.604282 | 3_4:+59382638 | None:intergenic |
| AACGAAGTTCGATCGGGAAC+CGG | 0.411671 | 3_4:+59378814 | None:intergenic |
| AACTTTGCATCTCTATCAAG+GGG | 0.623804 | 3_4:-59381205 | Msa0510350:CDS |
| AAGAAGAAGATTGAGGAAAT+GGG | 0.426271 | 3_4:-59382484 | Msa0510350:CDS |
| AAGATTATCACCCTGGGAGG+AGG | 0.522356 | 3_4:-59382016 | Msa0510350:CDS |
| AAGCAAGGATGCTCGCGAAA+AGG | 0.501565 | 3_4:+59370819 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTGAAGAGATAAGAATTG+GGG | + | chr3_4:59369603-59369622 | None:intergenic | 30.0% | |
| GAAAGTGAAGAGATAAGAAT+TGG | + | chr3_4:59369605-59369624 | None:intergenic | 30.0% | |
| AAGAAGAAGATTGAGGAAAT+GGG | - | chr3_4:59369961-59369980 | Msa0510350:CDS | 30.0% | |
| GGAAGCTAATAAGGATTTAT+TGG | - | chr3_4:59370275-59370294 | Msa0510350:intron | 30.0% | |
| GCTAATAAGGATTTATTGGA+AGG | - | chr3_4:59370279-59370298 | Msa0510350:intron | 30.0% | |
| TGAGAGAGTAAAATTGTTCT+GGG | - | chr3_4:59370536-59370555 | Msa0510350:intron | 30.0% | |
| GAATTGTGAAATAGCTTACT+TGG | + | chr3_4:59370618-59370637 | None:intergenic | 30.0% | |
| TGAAAACAGACTAATACCAT+AGG | + | chr3_4:59370731-59370750 | None:intergenic | 30.0% | |
| GTTTCGAATTACTGCTATTA+TGG | - | chr3_4:59370764-59370783 | Msa0510350:intron | 30.0% | |
| TAACCGAATTTACCCTTTAA+TGG | - | chr3_4:59370968-59370987 | Msa0510350:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 59369563 | 59382904 | 59369563 | ID=Msa0510350;Name=Msa0510350 |
| chr3_4 | mRNA | 59369563 | 59382904 | 59369563 | ID=Msa0510350-mRNA-1;Parent=Msa0510350;Name=Msa0510350-mRNA-1;_AED=0.04;_eAED=0.04;_QI=26|0.93|0.81|1|0.86|0.93|16|232|744 |
| chr3_4 | exon | 59369563 | 59370031 | 59369563 | ID=Msa0510350-mRNA-1:exon:13870;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59370790 | 59370972 | 59370790 | ID=Msa0510350-mRNA-1:exon:13869;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59371143 | 59371193 | 59371143 | ID=Msa0510350-mRNA-1:exon:13868;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59371399 | 59371518 | 59371399 | ID=Msa0510350-mRNA-1:exon:13867;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59372057 | 59372159 | 59372057 | ID=Msa0510350-mRNA-1:exon:13866;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59372537 | 59372643 | 59372537 | ID=Msa0510350-mRNA-1:exon:13865;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59372990 | 59373040 | 59372990 | ID=Msa0510350-mRNA-1:exon:13864;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59376122 | 59376298 | 59376122 | ID=Msa0510350-mRNA-1:exon:13863;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59378270 | 59378362 | 59378270 | ID=Msa0510350-mRNA-1:exon:13862;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59378597 | 59378663 | 59378597 | ID=Msa0510350-mRNA-1:exon:13861;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59378786 | 59378859 | 59378786 | ID=Msa0510350-mRNA-1:exon:13860;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59380408 | 59380489 | 59380408 | ID=Msa0510350-mRNA-1:exon:13859;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59381174 | 59381247 | 59381174 | ID=Msa0510350-mRNA-1:exon:13858;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59381849 | 59381878 | 59381849 | ID=Msa0510350-mRNA-1:exon:13857;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59381986 | 59382045 | 59381986 | ID=Msa0510350-mRNA-1:exon:13856;Parent=Msa0510350-mRNA-1 |
| chr3_4 | exon | 59382153 | 59382904 | 59382153 | ID=Msa0510350-mRNA-1:exon:13855;Parent=Msa0510350-mRNA-1 |
| chr3_4 | five_prime_UTR | 59382879 | 59382904 | 59382879 | ID=Msa0510350-mRNA-1:five_prime_utr;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59382153 | 59382878 | 59382153 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59381986 | 59382045 | 59381986 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59381849 | 59381878 | 59381849 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59381174 | 59381247 | 59381174 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59380408 | 59380489 | 59380408 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59378786 | 59378859 | 59378786 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59378597 | 59378663 | 59378597 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59378270 | 59378362 | 59378270 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59376122 | 59376298 | 59376122 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59372990 | 59373040 | 59372990 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59372537 | 59372643 | 59372537 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59372057 | 59372159 | 59372057 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59371399 | 59371518 | 59371399 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59371143 | 59371193 | 59371143 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59370790 | 59370972 | 59370790 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | CDS | 59369795 | 59370031 | 59369795 | ID=Msa0510350-mRNA-1:cds;Parent=Msa0510350-mRNA-1 |
| chr3_4 | three_prime_UTR | 59369563 | 59369794 | 59369563 | ID=Msa0510350-mRNA-1:three_prime_utr;Parent=Msa0510350-mRNA-1 |
| Gene Sequence |
>Msa0510350 ATGCAAGTCTTCCCCAATTCTTATCTCTTCACTTTCCCTAAACCCTTAAACCTTAAACCCTCTCCCTTATTCCACCGTCATCCACCACCTCTACTCCACCGCAAACCACCTCTCACCACCGTCGCTAAATCCTTCACCTCCGATGAATTCCCCGTCGACGAAACCTTCCTCCAAACCTTCGGCCCTAAAACCAAAGAATCTGAAGACGAAGCACGTCGCCGCAACTGGATCGAACGCGGTTGGGCTCCATGGGAAGAGATTCTCACTCCTGAAGCCGAATTCGCGCGGACGAGTCTCAACGAAGGTGAAGAAGTTCCTCTGAGCTCGCCGGAGTCGATTGAAGCTTTTAGAATGTTGAACCCTAGGTATAGGAAGAAGAAGATTGAGGAAATGGGAGTGACGGAAGAGGAGTATTTGGCGAAGCAGTTTGAGATTAAGGAAGATGTTCCGGAGGAGTTGAAGACTAAATGGGCAGGACCGTTGGTGGTGAAGTTGGTTCCGCCGAGGGATTGGCCTCCAAGAGGGTGGGAGGTGGATCGAGAAGAGCTTGCGTTTATTCGTGAAGCGCATAAGATGGAGGCGAAGAGAGTGAGGTTGGAGGATATTGAAAATGGTGTTAGAACTGAAACTGATGATGTGTGTTTGGATAGGTATAAGGTGTTTTTGAAACAGTATAATGAGTGGGTGGAAGCTAATAAGGATTTATTGGAAGGGGAATCTTATAAGTTAGACCAAGATTATCACCCTGGGAGGAGGAAAAGAGGCAAGGATTACAAGGATGGCATGTACGAGCTACCATTCTATTATCCTGGCCAAATTTGTGTGGGGAAGGTGACAACTTTGCATCTCTATCAAGGGGCATTTGTTGACATTGGTGGAGTATATGATGGGTGGGTTCCTATAAAGAACAATGATTGGTTTTGGATTCGCCATCACATAAAAGTGGGTATGACCGTCATTGTTGAAATTACGGCAAAGCGAGATCCTTATCGCTTCCGGTTCCCGATCGAACTTCGTTTTGTGGATCCAAATATAGATCATCTGATCTTTAACAGATTTGACTTCCCACCAATATTTCACCGTGACGAAGATACTAATCCAGATGAATTGCGGCGTGACTGTGGAAGGCCTCCTATTCCTAGAAAAGATCCAGGAGACAAGCCAGAGGAAGAACCTCTATTTTCTAATCACCCATATGTTGAAAAGTTGTGGCAGATTAATGTAGCTGAGCAAATGATTTTGAATGATATGGATATTAATCCTGATAAATATAAAGGCAAAAAGCTATCAGACTTGGTTGACGAAGATGATTTTGATGAAGAAAAATCAGTTCAGCAGACAAAAGTTCCTTATAAGAATGCATTAGTACCAAAAATAACTATGAAAACAAGTGTTAAAGAGCTGGACTTAGAGGCTGCACTTGCTGAGCGTGAGGGAGGTGCTGTGACATATGTTTTGCTAACGCTTAGCAGTTGGTTCCTTGTCATTTCATGGCTTTTTGCTCCATACATTTTCAACCCTTCTGGTTTCGAGTGGCAAAAGACTGTGGAAGATTTTGATGATTGGACAAGTTGGCTCCTTTACAAAGGAGGAGTAGGAGTGAAAGGAGAAAATAGTTGGGAGTCTTGGTGGCTGGAAGAACAGATGCATATCCAAACTTTGAGGGGTCGTATCTTTGAGACGATTTTGAGTTTAAGGTTCTTCTTGTTTCAATATGGTGTTGTGTATAAGTTACATCTTACTGGAGATGATACATCACTTGCGATATATGGATTCTCGTGGGTTGTATTGGTTGGATTTGTCTTGATATTTAAGATCTTTACTTACAGCCCAAAAAAATCGGCCGATTTTCAGCTTGTATTAAGATTTTTACAGGGAGTTGTATCTATAGGCCTTGTTGCAGCTGTGTGTCTTGTTGTTGCTTTTACTCAACTTTCAATACCTGACCTTTTCGCGAGCATCCTTGCTTTTATCCCTACTGGATGGGGAATATTATCTTTGGCAATTACATGGAAGGGTATTATGAAGACTCTTGGCCTATGGGAATCTGTCCGTGAGTTTGCTAGAATGTATGATGCTGGAATGGGTATGATTATTTTTTCACCAATAGCATTTTTATCATGGTTTCCGTTCATTTCCACTTTCCAGTCTCGTCTTCTATTTAATCAAGCCTTCAGCCGTGGTCTTGAAATCTCCCTCATCCTTTCTGGAAACAAAGCTAACGTTGAGACATAA |
| Protein sequence |
>Msa0510350 MQVFPNSYLFTFPKPLNLKPSPLFHRHPPPLLHRKPPLTTVAKSFTSDEFPVDETFLQTFGPKTKESEDEARRRNWIERGWAPWEEILTPEAEFARTSLNEGEEVPLSSPESIEAFRMLNPRYRKKKIEEMGVTEEEYLAKQFEIKEDVPEELKTKWAGPLVVKLVPPRDWPPRGWEVDREELAFIREAHKMEAKRVRLEDIENGVRTETDDVCLDRYKVFLKQYNEWVEANKDLLEGESYKLDQDYHPGRRKRGKDYKDGMYELPFYYPGQICVGKVTTLHLYQGAFVDIGGVYDGWVPIKNNDWFWIRHHIKVGMTVIVEITAKRDPYRFRFPIELRFVDPNIDHLIFNRFDFPPIFHRDEDTNPDELRRDCGRPPIPRKDPGDKPEEEPLFSNHPYVEKLWQINVAEQMILNDMDINPDKYKGKKLSDLVDEDDFDEEKSVQQTKVPYKNALVPKITMKTSVKELDLEAALAEREGGAVTYVLLTLSSWFLVISWLFAPYIFNPSGFEWQKTVEDFDDWTSWLLYKGGVGVKGENSWESWWLEEQMHIQTLRGRIFETILSLRFFLFQYGVVYKLHLTGDDTSLAIYGFSWVVLVGFVLIFKIFTYSPKKSADFQLVLRFLQGVVSIGLVAAVCLVVAFTQLSIPDLFASILAFIPTGWGILSLAITWKGIMKTLGLWESVREFARMYDAGMGMIIFSPIAFLSWFPFISTFQSRLLFNQAFSRGLEISLILSGNKANVET* |
