AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0636070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0636070 MtrunA17_Chr4g0018731 66.667 177 42 5 1 165 1 172 2.61e-40 134
Msa0636070 MtrunA17_Chr1g0168301 81.897 116 17 1 66 177 210 325 5.07e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGTGTTTAAGACTAAC+AGG 0.286414 4_3:+25866461 Msa0636070:CDS
ATCATCAACTGACAGTGATT+CGG 0.382705 4_3:+25866699 Msa0636070:CDS
AAAGGAGGAGGAGGGAGCAC+AGG 0.386258 4_3:+25866512 Msa0636070:CDS
TCATCAACTGACAGTGATTC+GGG 0.386672 4_3:+25866700 Msa0636070:CDS
GATTCGGGTGATGGATATAA+TGG 0.404972 4_3:+25866715 Msa0636070:CDS
ACCATGGAGATGATGATTAG+AGG 0.412561 4_3:+25866788 Msa0636070:CDS
TGGATATAATGGTTCAAGTA+AGG 0.433386 4_3:+25866726 Msa0636070:CDS
CTATATTCATCACTATCATC+TGG 0.440092 4_3:-25866556 None:intergenic
GAGAGGTTCTTATAGAAAGA+AGG 0.453802 4_3:+25866368 Msa0636070:intron
TTGAGAGAGCTATTGCTAAA+GGG 0.463888 4_3:+25866330 Msa0636070:CDS
GCTAGTGATTCAGATGGTTC+CGG 0.468678 4_3:+25866487 Msa0636070:CDS
ACTGACAGTGATTCGGGTGA+TGG 0.486358 4_3:+25866706 Msa0636070:CDS
CTCCCTCCTCCTCCTTTCAC+CGG 0.487250 4_3:-25866506 None:intergenic
GTAGGAAAGAGAAGAGGAGA+AGG 0.494224 4_3:+25866596 Msa0636070:CDS
ACAAAGATGCTGACCGAAAG+AGG 0.498522 4_3:+25866415 Msa0636070:CDS
ATTCAGATGGTTCCGGTGAA+AGG 0.502557 4_3:+25866494 Msa0636070:CDS
GAGCTCAGAAGAGGAGGAAG+AGG 0.513498 4_3:+25866271 Msa0636070:CDS
ATTGAGAGAGCTATTGCTAA+AGG 0.513599 4_3:+25866329 Msa0636070:CDS
TCCTCTAATCATCATCTCCA+TGG 0.523954 4_3:-25866789 None:intergenic
GTTCCGGTGAAAGGAGGAGG+AGG 0.525308 4_3:+25866503 Msa0636070:CDS
ATGAATATAGACACAAGAAA+AGG 0.532593 4_3:+25866569 Msa0636070:CDS
GGATATAATGGTTCAAGTAA+GGG 0.549938 4_3:+25866727 Msa0636070:CDS
ATGGTTCCGGTGAAAGGAGG+AGG 0.558346 4_3:+25866500 Msa0636070:CDS
TAATGGTTCAAGTAAGGGTA+GGG 0.560844 4_3:+25866732 Msa0636070:CDS
TGTTGAGAGCTCAGAAGAGG+AGG 0.561377 4_3:+25866265 Msa0636070:CDS
GACACAAGAAAAGGAAGAGT+AGG 0.567421 4_3:+25866578 Msa0636070:CDS
TTCTGATTCTGAAACATCTG+AGG 0.573778 4_3:+25866636 Msa0636070:CDS
GGGGAAGTTAGTGAGAAGAG+AGG 0.575217 4_3:+25866351 Msa0636070:CDS
AGAGAGGTAGAGAAAAGAGA+AGG 0.579127 4_3:+25866442 Msa0636070:CDS
ATAATGGTTCAAGTAAGGGT+AGG 0.582111 4_3:+25866731 Msa0636070:CDS
TGAGAGAGCTATTGCTAAAG+GGG 0.588008 4_3:+25866331 Msa0636070:CDS
GGAAGAGTAGGAAAGAGAAG+AGG 0.590104 4_3:+25866590 Msa0636070:CDS
GGGAGCACAGGAGAAGCATG+AGG 0.590249 4_3:+25866524 Msa0636070:CDS
CAATGTTGAGAGCTCAGAAG+AGG 0.592360 4_3:+25866262 None:intergenic
AAGAGGAGGAGACGCCACCA+TGG 0.594257 4_3:+25866772 Msa0636070:CDS
CAGATGGTTCCGGTGAAAGG+AGG 0.617536 4_3:+25866497 Msa0636070:CDS
CATCTGAGGATTCTACTTCG+CGG 0.617958 4_3:+25866650 Msa0636070:CDS
AGGGAAGCTAGTGATTCAGA+TGG 0.649064 4_3:+25866481 Msa0636070:CDS
GGAGCACAGGAGAAGCATGA+GGG 0.656858 4_3:+25866525 Msa0636070:CDS
TTCCGGTGAAAGGAGGAGGA+GGG 0.666380 4_3:+25866504 Msa0636070:CDS
AGGAGTGTTTAAGACTAACA+GGG 0.683554 4_3:+25866462 Msa0636070:CDS
TCTAATCATCATCTCCATGG+TGG 0.691101 4_3:-25866786 None:intergenic
GAGAGAGCTATTGCTAAAGG+GGG 0.696345 4_3:+25866332 Msa0636070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAAAAAATCAGAAAAG+AGG + chr4_3:25866755-25866774 Msa0636070:CDS 20.0%
! ATGAATATAGACACAAGAAA+AGG + chr4_3:25866569-25866588 Msa0636070:CDS 25.0%
CTATATTCATCACTATCATC+TGG - chr4_3:25866559-25866578 None:intergenic 30.0%
TGGATATAATGGTTCAAGTA+AGG + chr4_3:25866726-25866745 Msa0636070:CDS 30.0%
GGATATAATGGTTCAAGTAA+GGG + chr4_3:25866727-25866746 Msa0636070:CDS 30.0%
TCAAAAAATCAGAAAAGAGG+AGG + chr4_3:25866758-25866777 Msa0636070:CDS 30.0%
ATTGAGAGAGCTATTGCTAA+AGG + chr4_3:25866329-25866348 Msa0636070:CDS 35.0%
TTGAGAGAGCTATTGCTAAA+GGG + chr4_3:25866330-25866349 Msa0636070:CDS 35.0%
GAGAGGTTCTTATAGAAAGA+AGG + chr4_3:25866368-25866387 Msa0636070:intron 35.0%
! TACCTCTCTTTTTCCTCTTT+CGG - chr4_3:25866431-25866450 None:intergenic 35.0%
!! AAGGAGTGTTTAAGACTAAC+AGG + chr4_3:25866461-25866480 Msa0636070:CDS 35.0%
!! AGGAGTGTTTAAGACTAACA+GGG + chr4_3:25866462-25866481 Msa0636070:CDS 35.0%
!! TTCTGATTCTGAAACATCTG+AGG + chr4_3:25866636-25866655 Msa0636070:CDS 35.0%
ATCATCAACTGACAGTGATT+CGG + chr4_3:25866699-25866718 Msa0636070:CDS 35.0%
ATAATGGTTCAAGTAAGGGT+AGG + chr4_3:25866731-25866750 Msa0636070:CDS 35.0%
TAATGGTTCAAGTAAGGGTA+GGG + chr4_3:25866732-25866751 Msa0636070:CDS 35.0%
TGAGAGAGCTATTGCTAAAG+GGG + chr4_3:25866331-25866350 Msa0636070:CDS 40.0%
AGAGAGGTAGAGAAAAGAGA+AGG + chr4_3:25866442-25866461 Msa0636070:CDS 40.0%
GACACAAGAAAAGGAAGAGT+AGG + chr4_3:25866578-25866597 Msa0636070:CDS 40.0%
TCATCAACTGACAGTGATTC+GGG + chr4_3:25866700-25866719 Msa0636070:CDS 40.0%
!! GATTCGGGTGATGGATATAA+TGG + chr4_3:25866715-25866734 Msa0636070:CDS 40.0%
GAGAGAGCTATTGCTAAAGG+GGG + chr4_3:25866332-25866351 Msa0636070:CDS 45.0%
ACAAAGATGCTGACCGAAAG+AGG + chr4_3:25866415-25866434 Msa0636070:CDS 45.0%
GACCGAAAGAGGAAAAAGAG+AGG + chr4_3:25866426-25866445 Msa0636070:CDS 45.0%
AGGGAAGCTAGTGATTCAGA+TGG + chr4_3:25866481-25866500 Msa0636070:CDS 45.0%
GCTAGTGATTCAGATGGTTC+CGG + chr4_3:25866487-25866506 Msa0636070:CDS 45.0%
ATTCAGATGGTTCCGGTGAA+AGG + chr4_3:25866494-25866513 Msa0636070:CDS 45.0%
GGAAGAGTAGGAAAGAGAAG+AGG + chr4_3:25866590-25866609 Msa0636070:CDS 45.0%
GTAGGAAAGAGAAGAGGAGA+AGG + chr4_3:25866596-25866615 Msa0636070:CDS 45.0%
CATCTGAGGATTCTACTTCG+CGG + chr4_3:25866650-25866669 Msa0636070:CDS 45.0%
TGTTGAGAGCTCAGAAGAGG+AGG + chr4_3:25866265-25866284 Msa0636070:CDS 50.0%
GGGGAAGTTAGTGAGAAGAG+AGG + chr4_3:25866351-25866370 Msa0636070:CDS 50.0%
! ACTGACAGTGATTCGGGTGA+TGG + chr4_3:25866706-25866725 Msa0636070:CDS 50.0%
GAGCTCAGAAGAGGAGGAAG+AGG + chr4_3:25866271-25866290 Msa0636070:CDS 55.0%
CAGATGGTTCCGGTGAAAGG+AGG + chr4_3:25866497-25866516 Msa0636070:CDS 55.0%
ATGGTTCCGGTGAAAGGAGG+AGG + chr4_3:25866500-25866519 Msa0636070:CDS 55.0%
TTCCGGTGAAAGGAGGAGGA+GGG + chr4_3:25866504-25866523 Msa0636070:CDS 55.0%
! GGAGCACAGGAGAAGCATGA+GGG + chr4_3:25866525-25866544 Msa0636070:CDS 55.0%
GTTCCGGTGAAAGGAGGAGG+AGG + chr4_3:25866503-25866522 Msa0636070:CDS 60.0%
CTCCCTCCTCCTCCTTTCAC+CGG - chr4_3:25866509-25866528 None:intergenic 60.0%
! AAAGGAGGAGGAGGGAGCAC+AGG + chr4_3:25866512-25866531 Msa0636070:CDS 60.0%
! GGGAGCACAGGAGAAGCATG+AGG + chr4_3:25866524-25866543 Msa0636070:CDS 60.0%
AAGAGGAGGAGACGCCACCA+TGG + chr4_3:25866772-25866791 Msa0636070:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 25866264 25866807 25866264 ID=Msa0636070;Name=Msa0636070
chr4_3 mRNA 25866264 25866807 25866264 ID=Msa0636070-mRNA-1;Parent=Msa0636070;Name=Msa0636070-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|0|0|2|0|177
chr4_3 exon 25866264 25866372 25866264 ID=Msa0636070-mRNA-1:exon:6498;Parent=Msa0636070-mRNA-1
chr4_3 exon 25866383 25866807 25866383 ID=Msa0636070-mRNA-1:exon:6499;Parent=Msa0636070-mRNA-1
chr4_3 CDS 25866264 25866372 25866264 ID=Msa0636070-mRNA-1:cds;Parent=Msa0636070-mRNA-1
chr4_3 CDS 25866383 25866807 25866383 ID=Msa0636070-mRNA-1:cds;Parent=Msa0636070-mRNA-1
Gene Sequence

>Msa0636070

ATGTTGAGAGCTCAGAAGAGGAGGAAGAGGATAGTGAGAGTTCAGATTCTGAGATTGATTCAGAGATTGAGAGAGCTATTGCTAAAGGGGGAAGTTAGTGAGAAGAGAGAAAGAAGGATGACTAAGATGATGACGAGTCAGACAAAGATGCTGACCGAAAGAGGAAAAAGAGAGGTAGAGAAAAGAGAAGGAGTGTTTAAGACTAACAGGGAAGCTAGTGATTCAGATGGTTCCGGTGAAAGGAGGAGGAGGGAGCACAGGAGAAGCATGAGGGACGAGTCTCCAGATGATAGTGATGAATATAGACACAAGAAAAGGAAGAGTAGGAAAGAGAAGAGGAGAAGGAGAAGTCGTCTTTCAGATTCTGATTCTGAAACATCTGAGGATTCTACTTCGCGGCACAAGCGAAATAGCAAGAGAACTTCATCATCAACTGACAGTGATTCGGGTGATGGATATAATGGTTCAAGTAAGGGTAGGGATATCAAAAAATCAGAAAAGAGGAGGAGACGCCACCATGGAGATGATGATTAG

Protein sequence

>Msa0636070

MLRAQKRRKRIVRVQILRLIQRLRELLLKGEVSEKRERRMTKMMTSQTKMLTERGKREVEKREGVFKTNREASDSDGSGERRRREHRRSMRDESPDDSDEYRHKKRKSRKEKRRRRSRLSDSDSETSEDSTSRHKRNSKRTSSSTDSDSGDGYNGSSKGRDIKKSEKRRRRHHGDDD*