AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0970820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970820 MtrunA17_Chr6g0449731 91.089 202 6 1 1 190 1 202 4.79e-126 361
Msa0970820 MtrunA17_Chr6g0449731 80.000 180 35 1 193 371 40 219 2.61e-93 278
Msa0970820 MtrunA17_Chr6g0449771 91.089 202 6 1 1 190 1 202 7.02e-126 361
Msa0970820 MtrunA17_Chr6g0449771 80.663 181 34 1 193 372 40 220 7.83e-95 282
Msa0970820 MtrunA17_Chr6g0449761 89.604 202 9 1 1 190 1 202 4.90e-124 357
Msa0970820 MtrunA17_Chr6g0449761 80.226 177 34 1 193 368 40 216 1.55e-91 275
Msa0970820 MtrunA17_Chr6g0449751 88.119 202 12 1 1 190 1 202 2.03e-121 350
Msa0970820 MtrunA17_Chr6g0449751 78.804 184 38 1 190 372 37 220 5.15e-94 280
Msa0970820 MtrunA17_Chr6g0449781 85.930 199 28 0 174 372 21 219 9.85e-121 348
Msa0970820 MtrunA17_Chr6g0449781 68.317 202 51 2 1 190 1 201 1.33e-85 258
Msa0970820 MtrunA17_Chr1g0189841 78.392 199 28 3 7 190 6 204 2.55e-102 301
Msa0970820 MtrunA17_Chr1g0189841 68.817 186 55 3 190 372 37 222 1.43e-77 238
Msa0970820 MtrunA17_Chr2g0285871 74.500 200 37 2 5 190 4 203 4.10e-98 290
Msa0970820 MtrunA17_Chr2g0285871 68.132 182 56 2 193 372 40 221 1.65e-75 233
Msa0970820 MtrunA17_Chr4g0007031 74.000 200 38 2 5 190 4 203 8.60e-98 290
Msa0970820 MtrunA17_Chr4g0007031 68.132 182 56 2 193 372 40 221 9.72e-76 233
Msa0970820 MtrunA17_Chr5g0419971 74.000 200 38 2 5 190 4 203 8.60e-98 290
Msa0970820 MtrunA17_Chr5g0419971 68.132 182 56 2 193 372 40 221 9.72e-76 233
Msa0970820 MtrunA17_Chr5g0419981 74.000 200 38 2 5 190 4 203 8.60e-98 290
Msa0970820 MtrunA17_Chr5g0419981 68.132 182 56 2 193 372 40 221 9.72e-76 233
Msa0970820 MtrunA17_Chr4g0007041 72.414 203 43 1 1 190 1 203 8.69e-98 290
Msa0970820 MtrunA17_Chr4g0007041 68.681 182 55 2 193 372 40 221 6.80e-76 234
Msa0970820 MtrunA17_Chr4g0007051 74.000 200 38 2 5 190 4 203 9.08e-98 290
Msa0970820 MtrunA17_Chr4g0007051 68.132 182 56 2 193 372 40 221 9.61e-76 233
Msa0970820 MtrunA17_Chr6g0449791 66.995 203 54 1 1 190 1 203 3.36e-89 267
Msa0970820 MtrunA17_Chr6g0449791 68.539 178 54 2 184 359 31 208 2.03e-76 234
Msa0970820 MtrunA17_Chr1g0156131 58.602 186 55 3 19 190 19 196 2.64e-65 206
Msa0970820 MtrunA17_Chr1g0156131 57.303 178 72 3 193 367 33 209 2.18e-61 196
Msa0970820 MtrunA17_Chr2g0292831 58.602 186 58 3 19 190 19 199 4.78e-65 206
Msa0970820 MtrunA17_Chr2g0292831 56.000 175 73 3 196 367 39 212 6.74e-57 184
Msa0970820 MtrunA17_Chr2g0292901 57.754 187 59 4 19 190 19 200 6.50e-62 197
Msa0970820 MtrunA17_Chr2g0292901 56.571 175 72 3 196 367 40 213 8.46e-58 187
Msa0970820 MtrunA17_Chr2g0292861 60.377 159 48 3 47 190 7 165 4.62e-57 184
Msa0970820 MtrunA17_Chr2g0292861 55.307 179 75 4 193 367 1 178 8.51e-55 178
Msa0970820 MtrunA17_Chr2g0292841 60.377 159 48 3 47 190 7 165 8.71e-56 181
Msa0970820 MtrunA17_Chr2g0292841 54.190 179 77 4 193 367 1 178 8.00e-53 173
Msa0970820 MtrunA17_Chr1g0193671 46.154 195 87 4 12 190 9 201 8.64e-49 164
Msa0970820 MtrunA17_Chr1g0193671 43.077 195 106 3 175 366 21 213 3.00e-45 155
Msa0970820 MtrunA17_Chr7g0271211 51.381 181 74 3 22 190 20 198 1.25e-47 160
Msa0970820 MtrunA17_Chr7g0271211 42.564 195 107 3 176 367 19 211 9.86e-39 137
Msa0970820 MtrunA17_Chr1g0193421 47.568 185 83 3 18 190 20 202 5.07e-46 157
Msa0970820 MtrunA17_Chr1g0193421 39.594 197 114 3 174 367 21 215 8.83e-38 135
Msa0970820 MtrunA17_Chr2g0292911 60.484 124 46 2 193 314 1 123 6.45e-43 146
Msa0970820 MtrunA17_Chr2g0292911 59.322 118 34 2 47 150 7 124 1.48e-39 137
Msa0970820 MtrunA17_Chr5g0414971 45.263 190 85 5 22 198 27 210 6.92e-43 149
Msa0970820 MtrunA17_Chr5g0414971 41.011 178 99 4 194 367 44 219 4.47e-28 109
Msa0970820 MtrunA17_Chr1g0210761 43.000 200 91 6 2 189 5 193 1.21e-38 137
Msa0970820 MtrunA17_Chr1g0210761 48.462 130 64 2 238 367 81 207 3.50e-31 117
Msa0970820 MtrunA17_Chr8g0344021 42.021 188 84 5 5 177 7 184 8.30e-35 127
Msa0970820 MtrunA17_Chr8g0344021 42.500 160 87 2 196 353 39 195 3.29e-31 117
Msa0970820 MtrunA17_Chr1g0178711 38.660 194 101 4 12 189 16 207 5.79e-34 125
Msa0970820 MtrunA17_Chr1g0178711 36.458 192 114 4 177 367 37 221 4.98e-29 112
Msa0970820 MtrunA17_Chr2g0300561 41.270 189 90 4 1 177 3 182 2.12e-33 123
Msa0970820 MtrunA17_Chr2g0300561 39.412 170 98 3 197 364 37 203 3.83e-27 107
Msa0970820 MtrunA17_Chr2g0300581 38.624 189 95 4 1 177 3 182 9.68e-31 116
Msa0970820 MtrunA17_Chr2g0300581 38.012 171 101 3 197 365 37 204 1.25e-27 108
Msa0970820 MtrunA17_Chr2g0300591 38.624 189 95 4 1 177 3 182 1.18e-30 116
Msa0970820 MtrunA17_Chr2g0300591 38.235 170 100 3 197 364 37 203 1.44e-27 108
Msa0970820 MtrunA17_Chr8g0335851 39.759 166 89 2 22 176 26 191 1.05e-28 111
Msa0970820 MtrunA17_Chr8g0335851 39.157 166 90 3 193 353 44 203 2.98e-23 96.7
Msa0970820 MtrunA17_Chr4g0026191 42.029 138 76 3 231 367 71 205 4.32e-28 109
Msa0970820 MtrunA17_Chr4g0026191 35.878 131 71 3 74 193 67 195 7.40e-17 78.6
Msa0970820 MtrunA17_Chr4g0026201 42.754 138 75 3 231 367 71 205 5.09e-28 108
Msa0970820 MtrunA17_Chr4g0026201 35.878 131 71 3 74 193 67 195 8.41e-17 78.2
Msa0970820 MtrunA17_Chr8g0343911 38.506 174 91 4 26 187 19 188 9.88e-28 108
Msa0970820 MtrunA17_Chr8g0343911 36.628 172 104 3 196 365 34 202 3.78e-25 101
Msa0970820 MtrunA17_Chr8g0343934 38.251 183 96 4 5 177 6 181 1.77e-27 107
Msa0970820 MtrunA17_Chr8g0343934 36.810 163 98 3 206 367 47 205 1.77e-23 97.1
Msa0970820 MtrunA17_Chr2g0321601 34.466 206 111 6 180 367 4 203 9.18e-27 105
Msa0970820 MtrunA17_Chr2g0321601 30.097 206 111 7 2 193 7 193 7.70e-17 78.6
Msa0970820 MtrunA17_Chr2g0321581 42.029 138 76 3 231 367 70 204 1.08e-26 105
Msa0970820 MtrunA17_Chr2g0321581 32.057 209 103 8 2 193 8 194 6.83e-18 81.3
Msa0970820 MtrunA17_Chr2g0321591 42.029 138 76 3 231 367 70 204 1.51e-26 105
Msa0970820 MtrunA17_Chr2g0321591 32.195 205 100 8 6 193 12 194 1.02e-17 80.9
Msa0970820 MtrunA17_Chr3g0084531 37.824 193 101 5 5 187 6 189 3.68e-26 104
Msa0970820 MtrunA17_Chr3g0084531 38.037 163 96 3 206 367 47 205 1.13e-23 97.4
Msa0970820 MtrunA17_Chr8g0343954 39.412 170 89 4 28 187 24 189 6.54e-26 103
Msa0970820 MtrunA17_Chr8g0343954 37.423 163 97 3 206 367 47 205 2.06e-23 96.7
Msa0970820 MtrunA17_Chr2g0313961 39.286 168 90 3 18 175 14 179 4.07e-25 101
Msa0970820 MtrunA17_Chr2g0313961 38.150 173 102 3 196 367 37 205 2.60e-24 99.4
Msa0970820 MtrunA17_Chr3g0084511 36.788 193 102 5 5 187 6 188 5.40e-25 100
Msa0970820 MtrunA17_Chr3g0084511 38.182 165 93 4 206 367 46 204 3.56e-23 95.9
Msa0970820 MtrunA17_Chr2g0297231 37.222 180 99 4 18 187 14 189 9.49e-25 100
Msa0970820 MtrunA17_Chr2g0297231 37.572 173 103 3 196 367 37 205 3.16e-24 99.0
Msa0970820 MtrunA17_Chr3g0130241 34.328 201 113 4 1 187 65 260 1.18e-24 102
Msa0970820 MtrunA17_Chr3g0130241 42.857 105 58 1 224 328 139 241 5.85e-18 83.2
Msa0970820 MtrunA17_Chr2g0297241 38.150 173 102 3 196 367 37 205 1.53e-24 99.8
Msa0970820 MtrunA17_Chr2g0297241 38.690 168 91 3 18 175 14 179 1.55e-24 99.8
Msa0970820 MtrunA17_Chr3g0084521 36.788 193 102 5 5 187 6 188 1.57e-24 99.8
Msa0970820 MtrunA17_Chr3g0084521 38.788 165 92 4 206 367 46 204 2.57e-24 99.4
Msa0970820 MtrunA17_Chr8g0344051 37.824 193 95 5 5 182 7 189 1.75e-24 99.8
Msa0970820 MtrunA17_Chr8g0344051 38.857 175 100 4 196 367 38 208 7.69e-24 97.8
Msa0970820 MtrunA17_Chr2g0302171 36.269 193 104 5 5 187 6 189 3.60e-24 99.0
Msa0970820 MtrunA17_Chr2g0302171 38.182 165 93 4 206 367 47 205 1.58e-23 97.1
Msa0970820 MtrunA17_Chr8g0344041 36.970 165 91 3 24 177 23 185 1.22e-23 97.4
Msa0970820 MtrunA17_Chr8g0344041 35.976 164 99 3 206 367 50 209 3.16e-22 93.6
Msa0970820 MtrunA17_Chr8g0343961 35.385 195 107 6 3 187 4 189 2.19e-23 96.7
Msa0970820 MtrunA17_Chr8g0343961 36.196 163 99 3 206 367 47 205 1.70e-21 91.7
Msa0970820 MtrunA17_Chr8g0343921 38.150 173 89 6 28 188 22 188 6.85e-23 95.1
Msa0970820 MtrunA17_Chr8g0343921 34.706 170 106 4 200 367 37 203 2.98e-19 85.1
Msa0970820 MtrunA17_Chr2g0301611 38.854 157 84 3 18 164 14 168 8.42e-23 95.1
Msa0970820 MtrunA17_Chr2g0301611 36.196 163 99 3 206 367 47 205 3.16e-22 93.6
Msa0970820 MtrunA17_Chr8g0344031 38.650 163 95 3 206 367 49 207 2.12e-22 94.0
Msa0970820 MtrunA17_Chr8g0344031 33.880 183 109 3 5 177 3 183 1.49e-20 89.0
Msa0970820 MtrunA17_Chr4g0003361 40.000 145 76 3 43 177 34 177 3.40e-22 93.2
Msa0970820 MtrunA17_Chr4g0003361 35.260 173 106 4 196 367 34 201 1.44e-20 89.0
Msa0970820 MtrunA17_Chr2g0286171 34.343 198 113 6 1 187 1 192 4.80e-21 90.5
Msa0970820 MtrunA17_Chr2g0286171 35.036 137 85 2 231 367 76 208 4.65e-18 82.0
Msa0970820 MtrunA17_Chr2g0292881 68.421 57 18 0 246 302 1 57 8.14e-21 85.1
Msa0970820 MtrunA17_Chr2g0292881 54.386 57 14 1 93 137 1 57 4.42e-11 57.8
Msa0970820 MtrunA17_Chr2g0302541 36.970 165 95 4 206 367 47 205 1.28e-20 89.0
Msa0970820 MtrunA17_Chr2g0302541 36.250 160 90 3 26 175 22 179 1.36e-19 86.3
Msa0970820 MtrunA17_Chr5g0411961 34.591 159 92 3 18 166 14 170 1.54e-20 89.0
Msa0970820 MtrunA17_Chr5g0411961 33.742 163 103 3 206 367 47 205 1.74e-19 85.9
Msa0970820 MtrunA17_Chr7g0217811 36.364 165 96 4 206 367 47 205 1.61e-20 89.0
Msa0970820 MtrunA17_Chr7g0217811 34.884 172 98 4 26 187 22 189 2.73e-19 85.5
Msa0970820 MtrunA17_Chr4g0012651 36.364 165 96 4 206 367 47 205 2.81e-20 88.2
Msa0970820 MtrunA17_Chr4g0012651 34.884 172 98 4 26 187 22 189 4.76e-19 84.7
Msa0970820 MtrunA17_Chr3g0115141 38.125 160 85 4 28 177 27 182 4.78e-20 87.4
Msa0970820 MtrunA17_Chr3g0115141 30.864 162 107 3 207 367 49 206 4.59e-15 73.6
Msa0970820 MtrunA17_Chr3g0084551 35.152 165 99 5 18 177 14 175 5.21e-16 76.6
Msa0970820 MtrunA17_Chr3g0084551 30.387 181 96 8 206 370 47 213 6.50e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970820 AT5G39120.1 61.165 206 64 3 1 190 1 206 1.57e-76 236
Msa0970820 AT5G39120.1 49.758 207 99 5 165 367 14 219 2.47e-53 176
Msa0970820 AT5G39150.1 60.680 206 65 3 1 190 1 206 1.23e-75 233
Msa0970820 AT5G39150.1 49.758 207 99 5 165 367 14 219 1.77e-53 176
Msa0970820 AT5G39180.1 60.287 209 67 3 1 193 1 209 2.26e-75 233
Msa0970820 AT5G39180.1 49.758 207 99 5 165 367 14 219 4.50e-53 175
Msa0970820 AT5G39110.1 60.891 202 64 2 4 190 6 207 5.85e-75 232
Msa0970820 AT5G39110.1 52.459 183 83 3 189 367 38 220 5.10e-54 178
Msa0970820 AT5G38940.1 63.934 183 52 2 22 190 26 208 1.31e-73 228
Msa0970820 AT5G38940.1 59.551 178 69 2 193 367 44 221 7.25e-62 198
Msa0970820 AT5G38940.2 63.934 183 52 2 22 190 50 232 1.99e-73 229
Msa0970820 AT5G38940.2 59.551 178 69 2 193 367 68 245 1.30e-61 198
Msa0970820 AT3G05950.1 63.590 195 54 4 12 190 15 208 8.71e-73 226
Msa0970820 AT3G05950.1 57.143 182 72 4 191 367 41 221 1.29e-55 182
Msa0970820 AT5G38930.1 63.243 185 54 2 20 190 24 208 9.95e-72 223
Msa0970820 AT5G38930.1 60.674 178 67 2 193 367 44 221 4.28e-62 199
Msa0970820 AT5G39160.1 62.903 186 53 3 20 190 22 206 1.26e-71 223
Msa0970820 AT5G39160.1 55.866 179 75 3 193 367 41 219 4.90e-56 183
Msa0970820 AT5G39190.1 62.903 186 53 3 20 190 22 206 2.15e-71 223
Msa0970820 AT5G39190.1 55.866 179 75 3 193 367 41 219 7.77e-56 182
Msa0970820 AT5G38960.1 61.702 188 57 2 18 190 22 209 2.97e-71 222
Msa0970820 AT5G38960.1 55.866 179 75 2 193 367 44 222 2.13e-55 181
Msa0970820 AT5G39130.1 61.828 186 55 3 20 190 22 206 6.61e-71 221
Msa0970820 AT5G39130.1 54.749 179 77 3 193 367 41 219 1.12e-54 179
Msa0970820 AT3G04200.1 59.606 203 63 4 3 190 11 209 2.64e-70 220
Msa0970820 AT3G04200.1 56.425 179 74 3 193 367 44 222 5.14e-51 170
Msa0970820 AT4G14630.1 60.317 189 60 2 20 193 24 212 2.58e-69 217
Msa0970820 AT4G14630.1 55.307 179 76 3 193 367 44 222 4.06e-55 181
Msa0970820 AT5G38910.1 56.306 222 71 6 1 206 1 212 4.25e-69 217
Msa0970820 AT5G38910.1 58.333 180 68 3 193 367 42 219 8.88e-57 185
Msa0970820 AT5G39160.3 60.215 186 54 3 20 190 22 202 7.95e-67 211
Msa0970820 AT5G39160.3 55.056 178 76 3 194 367 38 215 8.88e-54 177
Msa0970820 AT3G04190.1 56.316 190 67 3 15 189 19 207 2.70e-61 197
Msa0970820 AT3G04190.1 50.838 179 84 3 193 367 43 221 2.11e-43 150
Msa0970820 AT3G04150.1 53.846 208 76 5 1 189 1 207 1.16e-60 195
Msa0970820 AT3G04150.1 52.514 179 81 3 193 367 43 221 2.56e-47 160
Msa0970820 AT3G04150.2 50.455 220 78 5 1 189 1 220 1.96e-58 190
Msa0970820 AT3G04150.2 52.486 181 82 3 191 367 54 234 1.05e-47 162
Msa0970820 AT5G39190.2 55.435 184 48 2 20 190 22 184 1.72e-57 186
Msa0970820 AT5G39190.2 56.954 151 63 2 219 367 47 197 5.23e-49 164
Msa0970820 AT5G39160.2 54.891 184 49 2 20 190 22 184 4.08e-57 185
Msa0970820 AT5G39160.2 56.954 151 63 2 219 367 47 197 4.80e-49 164
Msa0970820 AT3G04180.1 53.684 190 72 4 15 189 19 207 8.98e-57 185
Msa0970820 AT3G04180.1 50.556 180 83 4 193 367 43 221 3.22e-43 150
Msa0970820 AT3G04170.1 52.821 195 72 5 11 189 14 204 1.34e-56 185
Msa0970820 AT3G04170.1 51.099 182 82 4 190 367 40 218 3.23e-45 155
Msa0970820 AT5G38910.2 52.381 210 61 6 1 206 1 175 3.09e-56 182
Msa0970820 AT5G38910.2 44.444 180 56 4 193 367 42 182 4.01e-32 119
Msa0970820 AT1G09560.1 49.451 182 78 3 22 191 24 203 1.93e-47 160
Msa0970820 AT1G09560.1 40.000 200 107 4 176 368 23 216 5.86e-41 144
Msa0970820 AT3G05930.1 47.059 204 90 5 1 189 1 201 3.02e-47 160
Msa0970820 AT3G05930.1 43.503 177 94 3 195 367 41 215 2.77e-39 139
Msa0970820 AT3G62020.1 47.716 197 88 4 7 190 8 202 1.07e-46 159
Msa0970820 AT3G62020.1 40.201 199 113 4 173 367 19 215 2.66e-38 137
Msa0970820 AT1G02335.1 46.995 183 83 3 20 190 22 202 3.77e-46 157
Msa0970820 AT1G02335.1 44.318 176 93 3 195 367 42 215 1.18e-40 143
Msa0970820 AT1G18970.1 44.162 197 94 4 7 191 14 206 1.73e-42 148
Msa0970820 AT1G18970.1 44.242 165 87 3 206 367 56 218 5.30e-35 128
Msa0970820 AT5G26700.1 41.327 196 105 4 176 367 20 209 3.11e-42 147
Msa0970820 AT5G26700.1 45.361 194 79 6 11 190 16 196 5.88e-42 146
Msa0970820 AT1G18980.1 46.703 182 81 4 22 191 29 206 2.45e-41 145
Msa0970820 AT1G18980.1 45.455 165 85 3 206 367 56 218 3.10e-35 129
Msa0970820 AT3G62020.2 49.091 165 71 3 39 190 9 173 1.76e-39 139
Msa0970820 AT3G62020.2 43.575 179 95 4 193 367 10 186 2.55e-38 136
Msa0970820 AT1G10460.1 40.104 192 96 6 22 198 19 206 1.53e-38 137
Msa0970820 AT1G10460.1 37.017 181 107 4 192 367 37 215 3.48e-28 110
Msa0970820 AT3G10080.1 39.459 185 96 4 19 189 23 205 8.24e-34 125
Msa0970820 AT3G10080.1 42.262 168 93 3 201 367 55 219 2.55e-31 119
Msa0970820 AT3G10080.2 39.247 186 97 4 18 189 93 276 2.35e-33 126
Msa0970820 AT3G10080.2 42.262 168 93 3 201 367 126 290 1.57e-30 118
Msa0970820 AT5G20630.1 38.095 189 97 4 1 177 3 183 2.16e-31 118
Msa0970820 AT5G20630.1 36.842 171 103 3 196 364 37 204 9.61e-25 100
Msa0970820 AT5G61750.1 37.186 199 106 5 5 191 7 198 1.19e-30 116
Msa0970820 AT5G61750.1 42.169 166 91 3 190 353 34 196 4.91e-29 112
Msa0970820 AT1G74820.1 35.468 203 107 5 8 190 14 212 4.04e-25 102
Msa0970820 AT1G74820.1 32.178 202 125 5 176 367 26 225 2.20e-22 94.7
Msa0970820 AT1G72610.1 37.805 164 88 3 26 177 19 180 6.80e-25 101
Msa0970820 AT1G72610.1 38.095 168 98 4 199 364 38 201 1.04e-24 100
Msa0970820 AT5G38950.1 67.797 59 19 0 132 190 2 60 2.38e-18 80.1
Msa0970820 AT5G38950.1 55.738 61 26 1 297 356 2 62 4.48e-12 62.4

Find 83 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATTGCTAATGCTGTATT+TGG 0.046653 6_4:+2464446 Msa0970820:CDS
CTTGTTTGTGACAGCCTTCT+TGG 0.121595 6_4:+2463730 Msa0970820:CDS
ACTATTGTTAAGGAGGTATT+TGG 0.136261 6_4:+2468147 Msa0970820:CDS
GGAAATGCAGCTGCCATTTC+CGG 0.176460 6_4:+2468096 Msa0970820:CDS
TAGTAATAACACCTGGATTT+TGG 0.212711 6_4:-2464427 Msa0970810:intron
TCATCTGGTGTAACAAGTTT+TGG 0.264444 6_4:-2467757 Msa0970810:intron
ACAATCTTCAGAAGCAATTC+TGG 0.265114 6_4:+2468232 Msa0970820:CDS
GCTACAGTTAGTGAGATATT+AGG 0.297571 6_4:+2467841 Msa0970820:CDS
TAGTGATAACTCTTGAATTA+TGG 0.314281 6_4:-2468128 Msa0970810:intron
TTCACTTTCAATTGAATGTT+GGG 0.325573 6_4:+2464369 Msa0970820:CDS
AGTATACATACCACCATTCT+TGG 0.343654 6_4:-2463822 Msa0970810:intron
CTTCTGAAGATTGTCAACTA+TGG 0.352917 6_4:-2468223 Msa0970810:intron
TCATTTGCTGTAGCAAGCTT+TGG 0.355521 6_4:-2464056 Msa0970810:intron
TTTATCCACTTGAAAAGCTT+TGG 0.360291 6_4:-2464501 Msa0970810:intron
AAGAGTTATCACTATTGTTA+AGG 0.372185 6_4:+2468137 Msa0970820:CDS
GAAATGCAGCTGCCATTTCC+GGG 0.374807 6_4:+2468097 Msa0970820:CDS
ATACTAGGACTAAACACTTT+AGG 0.375557 6_4:+2464155 Msa0970820:CDS
GATGTCTTTGTGTTTCCAAT+TGG 0.380749 6_4:+2468042 Msa0970820:CDS
GATGTCTTTGTGTTTCCAAT+TGG 0.393557 6_4:+2464341 Msa0970820:CDS
GGAAATGCAGTTGTCATTGC+TGG 0.408325 6_4:+2464395 Msa0970820:CDS
TAGTCCTAGTATCTCATTAA+CGG 0.408621 6_4:-2464144 Msa0970810:intron
CTATTGTTAAGGAGGTATTT+GGG 0.412780 6_4:+2468148 Msa0970820:CDS
TCGTATTGACTTTGCACCTA+AGG 0.416056 6_4:+2467891 Msa0970820:CDS
TGAAAGTGAATGAGGCCAAT+TGG 0.419001 6_4:-2464356 Msa0970810:intron
TGAGATACTTATAGTGTTGA+AGG 0.421959 6_4:+2467948 Msa0970820:CDS
CTATTGCTAATGCTGTATTT+GGG 0.423500 6_4:+2464447 Msa0970820:CDS
AGTATCTCATTAACGGTAAC+CGG 0.427049 6_4:-2464137 Msa0970810:intron
ATTCACTTTCAATTGAATGT+TGG 0.434824 6_4:+2464368 Msa0970820:CDS
TTTCAATTGAATGTTGGGTA+TGG 0.435827 6_4:+2464374 Msa0970820:CDS
TGGAAGTGAATAAGACCAAT+TGG 0.438371 6_4:-2468057 Msa0970810:intron
TGAATTATGGCTGTTAAGCC+CGG 0.446254 6_4:-2468115 Msa0970810:intron
ATGGCTGTTAAGCCCGGAAA+TGG 0.455636 6_4:-2468109 Msa0970810:intron
TGTGGAGGATTTAGACCCTT+AGG 0.459506 6_4:-2467907 Msa0970810:intron
TTCTTCTACTCGGTGAAAGA+AGG 0.467010 6_4:+2464080 Msa0970820:CDS
ATAAGAATCTCAGTGGCTCT+AGG 0.470121 6_4:-2464236 Msa0970810:intron
TGAGATTCTTATCGTGTTGG+AGG 0.479548 6_4:+2464247 Msa0970820:CDS
TCGCATTGACTTCGCTGCTA+GGG 0.481033 6_4:+2464190 Msa0970820:CDS
ATATTAGGATTAAATACTCT+TGG 0.484202 6_4:+2467856 Msa0970820:CDS
CGTATTGACTTTGCACCTAA+GGG 0.496446 6_4:+2467892 Msa0970820:CDS
ACAAAAGTCTTGTAGAGGAC+TGG 0.500872 6_4:-2463783 Msa0970810:intron
CTCGCATTGACTTCGCTGCT+AGG 0.500918 6_4:+2464189 Msa0970820:CDS
TATTCACTTCCAACATAATG+TGG 0.501651 6_4:+2468068 Msa0970820:CDS
ATAAGTATCTCAGCGGCTCT+AGG 0.503745 6_4:-2467937 Msa0970810:intron
TTGAAGGGTACTCTCAATGT+TGG 0.510254 6_4:+2467964 Msa0970820:CDS
CAAAAGTCTTGTAGAGGACT+GGG 0.538162 6_4:-2463782 Msa0970810:intron
CACTGAGATTCTTATCGTGT+TGG 0.542092 6_4:+2464244 Msa0970820:CDS
GTGGCTCTAGGGTGAGTATG+TGG 0.548107 6_4:-2464224 Msa0970810:intron
TAAGAATCTCAGTGGCTCTA+GGG 0.548530 6_4:-2464235 Msa0970810:intron
CGCATTGACTTCGCTGCTAG+GGG 0.548623 6_4:+2464191 Msa0970820:CDS
GGACTTAGCAGCCAAAATCC+AGG 0.549743 6_4:+2464416 Msa0970820:CDS
CATTCAATTGAAAGTGAATG+AGG 0.551568 6_4:-2464364 Msa0970810:intron
AGCAAATGATTTCTTCTACT+CGG 0.553402 6_4:+2464070 Msa0970820:CDS
ATTCACTTCCAACATAATGT+GGG 0.554540 6_4:+2468069 Msa0970820:CDS
TCTTCAGAAGCAATTCTGGT+TGG 0.562500 6_4:+2468236 Msa0970820:CDS
TTGTAGAGGACTGGGATCAA+AGG 0.567532 6_4:-2463774 Msa0970810:intron
ATTGTCCACTTGAAGAGCCT+TGG 0.568947 6_4:-2468199 Msa0970810:intron
GTTACCGTTAATGAGATACT+AGG 0.571440 6_4:+2464140 Msa0970820:CDS
TTCACTTCCAACATAATGTG+GGG 0.575295 6_4:+2468070 Msa0970820:CDS
TCTCACCAAGGCTCTTCAAG+TGG 0.580497 6_4:+2468194 Msa0970820:CDS
AGTTATCACTATTGTTAAGG+AGG 0.582820 6_4:+2468140 Msa0970820:CDS
TGTGAATGGCAAATTCTGCA+AGG 0.584711 6_4:+2464031 Msa0970820:CDS
ACATGATGCTAAAGCCAAGA+AGG 0.585634 6_4:-2463744 Msa0970810:intron
GAAAACATATCACGTCCACT+TGG 0.597398 6_4:+2467802 Msa0970820:CDS
GAGATACTTATAGTGTTGAA+GGG 0.608927 6_4:+2467949 Msa0970820:CDS
ATCAACGACATTAAGACTTG+GGG 0.609156 6_4:+2467222 Msa0970820:intron
GAGATTCTTATCGTGTTGGA+GGG 0.612927 6_4:+2464248 Msa0970820:CDS
ATTACAAAAGTTCTCAACAA+AGG 0.621502 6_4:+2468018 Msa0970820:CDS
TTCTTCAGAAGTTCTCACCA+AGG 0.626705 6_4:+2468182 Msa0970820:CDS
ATCAATGACACCAAGAATGG+TGG 0.627435 6_4:+2463812 Msa0970820:CDS
GCCACACAAAAGTCTTGTAG+AGG 0.632662 6_4:-2463788 Msa0970810:intron
GCTATCAATGACACCAAGAA+TGG 0.633041 6_4:+2463809 Msa0970820:CDS
ATCACAAAAGTACTCAACAA+AGG 0.644517 6_4:+2464317 Msa0970820:CDS
TCACTTCCAACATAATGTGG+GGG 0.647461 6_4:+2468071 Msa0970820:CDS
AGGACTGGGATCAAAGGCAA+AGG 0.648011 6_4:-2463768 Msa0970810:intron
AGTGTCACATGCGAACCAAG+TGG 0.659204 6_4:-2467817 Msa0970810:intron
GGAAACGCATCAAATCCACT+TGG 0.667487 6_4:+2464101 Msa0970820:CDS
ACTTGGTTCAAAAGTGACAC+CGG 0.669513 6_4:+2464118 Msa0970820:CDS
GCTCTAGGGTGAGTATGTGG+AGG 0.673911 6_4:-2464221 Msa0970810:intron
GCGGCTCTAGGATGAGCATG+TGG 0.681614 6_4:-2467925 Msa0970810:intron
GCTCTAGGATGAGCATGTGG+AGG 0.684016 6_4:-2467922 Msa0970810:intron
TTAGCAATAGTAATAACACC+TGG 0.687379 6_4:-2464434 Msa0970810:intron
CAACACTATAAGTATCTCAG+CGG 0.743612 6_4:-2467944 Msa0970810:intron
CAACACGATAAGAATCTCAG+TGG 0.757588 6_4:-2464243 Msa0970810:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAATTTATATATTTTT+TGG - chr6_4:2465908-2465927 Msa0970810:intron 0.0%
!!! ATTATAATTTATATATTTTT+TGG - chr6_4:2465908-2465927 Msa0970810:intron 0.0%
!!! TTATATATTTATATATTTTT+TGG - chr6_4:2466366-2466385 Msa0970810:intron 0.0%
!!! ATTATAATTTATATATTTTT+TGG - chr6_4:2465908-2465927 Msa0970810:intron 0.0%
!!! ATTATAATTTATATATTTTT+AGG - chr6_4:2465908-2465927 Msa0970810:intron 0.0%
!! AAACTTAAACAATAAATAAT+AGG - chr6_4:2464764-2464783 Msa0970810:intron 10.0%
!! AAAATTAATTATAACATTAG+AGG + chr6_4:2464958-2464977 Msa0970820:intron 10.0%
!!! TTGATTATTATTTTTATTAG+AGG - chr6_4:2465214-2465233 Msa0970810:intron 10.0%
!!! ATTTATTTTTTGTTGATAAA+TGG + chr6_4:2465556-2465575 Msa0970820:intron 10.0%
!! AATTATACAATATATATGAT+TGG + chr6_4:2465706-2465725 Msa0970820:intron 10.0%
!! ATTATACAATATATATGATT+GGG + chr6_4:2465707-2465726 Msa0970820:intron 10.0%
!!! TTTTAATAATTTAATGATTG+TGG + chr6_4:2465809-2465828 Msa0970820:intron 10.0%
!!! TATATTTATATATTTTTTGG+TGG - chr6_4:2466363-2466382 Msa0970810:intron 10.0%
!!! TTTTTGTAGATATTTTTTTT+TGG + chr6_4:2466812-2466831 Msa0970820:intron 10.0%
!! CTAGAATAAAATAAAATGTA+AGG + chr6_4:2464667-2464686 Msa0970820:intron 15.0%
!! AATAAAATAAAATGTAAGGT+TGG + chr6_4:2464671-2464690 Msa0970820:intron 15.0%
!!! AAAAAACGTTTATTTTCTAT+AGG + chr6_4:2464725-2464744 Msa0970820:intron 15.0%
!!! TTTTAATATGTTCGATAATA+TGG + chr6_4:2465146-2465165 Msa0970820:intron 15.0%
!!! TATAATTGAATGCTTTTTTA+GGG + chr6_4:2466278-2466297 Msa0970820:intron 15.0%
!!! TATAATTGAATGCTTTTTTA+GGG + chr6_4:2466278-2466297 Msa0970820:intron 15.0%
!!! TATAATTGAATGCTTTTTTA+GGG + chr6_4:2466278-2466297 Msa0970820:intron 15.0%
!!! TTACTTTAGAAATATTTTGA+TGG + chr6_4:2467508-2467527 Msa0970820:intron 15.0%
!! TGTATATATATCAATGTTGT+TGG - chr6_4:2463958-2463977 Msa0970810:intron 20.0%
!!! ATTTGTAACTTACTTTTGAT+AGG + chr6_4:2464829-2464848 Msa0970820:intron 20.0%
!!! TTTGTAACTTACTTTTGATA+GGG + chr6_4:2464830-2464849 Msa0970820:intron 20.0%
!!! AAAATACAACTTAGTTGATA+AGG + chr6_4:2464878-2464897 Msa0970820:intron 20.0%
!! AATAATTTAATGATTGTGGT+TGG + chr6_4:2465813-2465832 Msa0970820:intron 20.0%
!!! GTATAATTGAATGCTTTTTT+AGG + chr6_4:2466277-2466296 Msa0970820:intron 20.0%
!!! GTATAATTGAATGCTTTTTT+AGG + chr6_4:2466277-2466296 Msa0970820:intron 20.0%
!!! GTATAATTGAATGCTTTTTT+AGG + chr6_4:2466277-2466296 Msa0970820:intron 20.0%
!!! GATAAAATACATTTGACTTT+TGG - chr6_4:2467341-2467360 Msa0970810:intron 20.0%
!! AAATGTATTCGATCTAAATT+TGG - chr6_4:2467363-2467382 Msa0970810:intron 20.0%
!!! ATTGACATTTTTGAATTTGA+GGG + chr6_4:2467544-2467563 Msa0970820:intron 20.0%
!! ATGTGTTTAAATGCATATAT+AGG - chr6_4:2467648-2467667 Msa0970810:intron 20.0%
!! TATTTACTGTTATAAGAGAA+TGG - chr6_4:2467680-2467699 Msa0970810:intron 20.0%
!!! ATCAATTTATTTTTCAGTGT+TGG + chr6_4:2467711-2467730 Msa0970820:intron 20.0%
!! ATATTAGGATTAAATACTCT+TGG + chr6_4:2467856-2467875 Msa0970820:CDS 20.0%
! TTTATTCATCTTTACTCGTT+TGG + chr6_4:2463873-2463892 Msa0970820:intron 25.0%
! TCATAGTTCAATCAGTTTAA+CGG - chr6_4:2463905-2463924 Msa0970810:intron 25.0%
! ATTCACTTTCAATTGAATGT+TGG + chr6_4:2464368-2464387 Msa0970820:CDS 25.0%
! TTCACTTTCAATTGAATGTT+GGG + chr6_4:2464369-2464388 Msa0970820:CDS 25.0%
! TCAAGTGGATAAAAATATAG+TGG + chr6_4:2464511-2464530 Msa0970820:intron 25.0%
! ACAATCTTCAAAAACAATTC+TGG + chr6_4:2464534-2464553 Msa0970820:intron 25.0%
! CAATCTTCAAAAACAATTCT+GGG + chr6_4:2464535-2464554 Msa0970820:intron 25.0%
! GTAAATATAACAATATCGCA+AGG - chr6_4:2464640-2464659 Msa0970810:intron 25.0%
!! TCAACTAAGTTGTATTTTCA+AGG - chr6_4:2464876-2464895 Msa0970810:intron 25.0%
!!! TTATAACATTAGAGGTACTT+TGG + chr6_4:2464966-2464985 Msa0970820:intron 25.0%
!! TATTGCGTTTTTATACTTGT+AGG + chr6_4:2465026-2465045 Msa0970820:intron 25.0%
!! ATTGCGTTTTTATACTTGTA+GGG + chr6_4:2465027-2465046 Msa0970820:intron 25.0%
!!! ATGTAGTAAACTTTGAGATA+TGG - chr6_4:2465111-2465130 Msa0970810:intron 25.0%
! TGTGAAATCTCAAAATTGTT+AGG + chr6_4:2465258-2465277 Msa0970820:intron 25.0%
! ATCCAAAAATCTATCAAATG+TGG + chr6_4:2465629-2465648 Msa0970820:intron 25.0%
! TCCAAAAATCTATCAAATGT+GGG + chr6_4:2465630-2465649 Msa0970820:intron 25.0%
! TTCTAAACTCAACATATGAA+TGG - chr6_4:2465765-2465784 Msa0970810:intron 25.0%
! CATACTCATAATCATATCTA+GGG - chr6_4:2466699-2466718 Msa0970810:intron 25.0%
! ACATACTCATAATCATATCT+AGG - chr6_4:2466700-2466719 Msa0970810:intron 25.0%
! TGATTATGAGTATGTAAAAG+AGG + chr6_4:2466705-2466724 Msa0970820:intron 25.0%
! GATTATGAGTATGTAAAAGA+GGG + chr6_4:2466706-2466725 Msa0970820:intron 25.0%
!! CTTTTACATATGTAAAAGAG+TGG - chr6_4:2466787-2466806 Msa0970810:intron 25.0%
! AACAATCACGTAAAAGTTAA+TGG + chr6_4:2466881-2466900 Msa0970820:intron 25.0%
! TACTTTCACTGAAAATTGTA+CGG - chr6_4:2466973-2466992 Msa0970810:intron 25.0%
!! GTTTTATGAGAGATGATATT+TGG - chr6_4:2467045-2467064 Msa0970810:intron 25.0%
! GAAAAAGTGACTAATTTGAT+TGG - chr6_4:2467442-2467461 Msa0970810:intron 25.0%
!! GATTGACATTTTTGAATTTG+AGG + chr6_4:2467543-2467562 Msa0970820:intron 25.0%
!! CTTAGTTCTTGAAATTGTAA+GGG - chr6_4:2467612-2467631 Msa0970810:intron 25.0%
!! TCTTAGTTCTTGAAATTGTA+AGG - chr6_4:2467613-2467632 Msa0970810:intron 25.0%
! TGCATATATAGGAGAAAAAA+TGG - chr6_4:2467637-2467656 Msa0970810:intron 25.0%
! ATTACAAAAGTTCTCAACAA+AGG + chr6_4:2468018-2468037 Msa0970820:CDS 25.0%
! TAGTGATAACTCTTGAATTA+TGG - chr6_4:2468131-2468150 Msa0970810:intron 25.0%
! AAGAGTTATCACTATTGTTA+AGG + chr6_4:2468137-2468156 Msa0970820:CDS 25.0%
CATAGTTCAATCAGTTTAAC+GGG - chr6_4:2463904-2463923 Msa0970810:intron 30.0%
ACTATGATACAAAAGTCTCA+CGG + chr6_4:2463918-2463937 Msa0970820:intron 30.0%
! TGTTTTGCAGTTTTTGTGAA+TGG + chr6_4:2464017-2464036 Msa0970820:intron 30.0%
AGCAAATGATTTCTTCTACT+CGG + chr6_4:2464070-2464089 Msa0970820:CDS 30.0%
TAGTCCTAGTATCTCATTAA+CGG - chr6_4:2464147-2464166 Msa0970810:intron 30.0%
ATACTAGGACTAAACACTTT+AGG + chr6_4:2464155-2464174 Msa0970820:CDS 30.0%
!! ATCACAAAAGTACTCAACAA+AGG + chr6_4:2464317-2464336 Msa0970820:CDS 30.0%
CATTCAATTGAAAGTGAATG+AGG - chr6_4:2464367-2464386 Msa0970810:intron 30.0%
TTTCAATTGAATGTTGGGTA+TGG + chr6_4:2464374-2464393 Msa0970820:CDS 30.0%
! TAGTAATAACACCTGGATTT+TGG - chr6_4:2464430-2464449 Msa0970810:intron 30.0%
TTAGCAATAGTAATAACACC+TGG - chr6_4:2464437-2464456 Msa0970810:intron 30.0%
ACTATTGCTAATGCTGTATT+TGG + chr6_4:2464446-2464465 Msa0970820:CDS 30.0%
CTATTGCTAATGCTGTATTT+GGG + chr6_4:2464447-2464466 Msa0970820:CDS 30.0%
!! AGAACTTCTGAAGAGATTTT+AGG - chr6_4:2464479-2464498 Msa0970810:intron 30.0%
TTTATCCACTTGAAAAGCTT+TGG - chr6_4:2464504-2464523 Msa0970810:intron 30.0%
TCTCAAAGTTTACTACATTC+AGG + chr6_4:2465113-2465132 Msa0970820:intron 30.0%
CTCAAAGTTTACTACATTCA+GGG + chr6_4:2465114-2465133 Msa0970820:intron 30.0%
TCAAAGTTTACTACATTCAG+GGG + chr6_4:2465115-2465134 Msa0970820:intron 30.0%
ACAAGTTAAGAGAGAATATG+TGG - chr6_4:2465241-2465260 Msa0970810:intron 30.0%
!! TTCAAAAACTTGTTGATCTC+TGG + chr6_4:2465526-2465545 Msa0970820:intron 30.0%
CCAAAAATCTATCAAATGTG+GGG + chr6_4:2465631-2465650 Msa0970820:intron 30.0%
TTGAATGTTATTGCATGAGA+TGG + chr6_4:2466637-2466656 Msa0970820:intron 30.0%
AGTATGTAAAAGAGGGATTA+GGG + chr6_4:2466713-2466732 Msa0970820:intron 30.0%
TGTGTGCATTAATGATAAAG+AGG - chr6_4:2466943-2466962 Msa0970810:intron 30.0%
TTATCAACGACATTAAGACT+TGG + chr6_4:2467220-2467239 Msa0970820:intron 30.0%
TATCAACGACATTAAGACTT+GGG + chr6_4:2467221-2467240 Msa0970820:intron 30.0%
! TGGTTATATCTAAGATCAGA+TGG - chr6_4:2467321-2467340 Msa0970810:intron 30.0%
TTTCGTGTTTCATATAGTTG+AGG + chr6_4:2467572-2467591 Msa0970820:intron 30.0%
!!! TATTTTTCAGTGTTGGTGAA+CGG + chr6_4:2467718-2467737 Msa0970820:intron 30.0%
ACTGAGAAAAAGAAATCATC+TGG - chr6_4:2467775-2467794 Msa0970810:intron 30.0%
! TGAGATACTTATAGTGTTGA+AGG + chr6_4:2467948-2467967 Msa0970820:CDS 30.0%
! GAGATACTTATAGTGTTGAA+GGG + chr6_4:2467949-2467968 Msa0970820:CDS 30.0%
!!! TTGTAATGAGACGGTTTTTT+GGG - chr6_4:2468005-2468024 Msa0970810:intron 30.0%
!!! TTTGTAATGAGACGGTTTTT+TGG - chr6_4:2468006-2468025 Msa0970810:intron 30.0%
! TGAGAACTTTTGTAATGAGA+CGG - chr6_4:2468014-2468033 Msa0970810:intron 30.0%
TATTCACTTCCAACATAATG+TGG + chr6_4:2468068-2468087 Msa0970820:CDS 30.0%
ATTCACTTCCAACATAATGT+GGG + chr6_4:2468069-2468088 Msa0970820:CDS 30.0%
AGTTATCACTATTGTTAAGG+AGG + chr6_4:2468140-2468159 Msa0970820:CDS 30.0%
!! ACTATTGTTAAGGAGGTATT+TGG + chr6_4:2468147-2468166 Msa0970820:CDS 30.0%
!! CTATTGTTAAGGAGGTATTT+GGG + chr6_4:2468148-2468167 Msa0970820:CDS 30.0%
AGTATACATACCACCATTCT+TGG - chr6_4:2463825-2463844 Msa0970810:intron 35.0%
CTATGATACAAAAGTCTCAC+GGG + chr6_4:2463919-2463938 Msa0970820:intron 35.0%
AGTATCTCATTAACGGTAAC+CGG - chr6_4:2464140-2464159 Msa0970810:intron 35.0%
GTTACCGTTAATGAGATACT+AGG + chr6_4:2464140-2464159 Msa0970820:CDS 35.0%
! TGAGTACTTTTGTGATGAGA+CGG - chr6_4:2464313-2464332 Msa0970810:intron 35.0%
GATGTCTTTGTGTTTCCAAT+TGG + chr6_4:2464341-2464360 Msa0970820:CDS 35.0%
TCTTCAAAAACAATTCTGGG+TGG + chr6_4:2464538-2464557 Msa0970820:intron 35.0%
! ATGATAACGAAAGACTCTTC+TGG + chr6_4:2465466-2465485 Msa0970820:intron 35.0%
! CCCCACATTTGATAGATTTT+TGG - chr6_4:2465634-2465653 Msa0970810:intron 35.0%
! TAATGATTGTGGTTGGTTGA+TGG + chr6_4:2465820-2465839 Msa0970820:intron 35.0%
AATGTTATTGCATGAGATGG+AGG + chr6_4:2466640-2466659 Msa0970820:intron 35.0%
ATGTTATTGCATGAGATGGA+GGG + chr6_4:2466641-2466660 Msa0970820:intron 35.0%
CTCATAATCATATCTAGGGA+GGG - chr6_4:2466695-2466714 Msa0970810:intron 35.0%
ACTCATAATCATATCTAGGG+AGG - chr6_4:2466696-2466715 Msa0970810:intron 35.0%
GAGTATGTAAAAGAGGGATT+AGG + chr6_4:2466712-2466731 Msa0970820:intron 35.0%
!!! TAGATATTTTTTCGCGTGGA+GGG - chr6_4:2466754-2466773 Msa0970810:intron 35.0%
!!! TCTGTAGATATTTTTTCGCG+TGG - chr6_4:2466758-2466777 Msa0970810:intron 35.0%
AGGATTGGGATCAAATGTAA+AGG - chr6_4:2467181-2467200 Msa0970810:intron 35.0%
! CAAAAGTTTTCCAGAGGATT+GGG - chr6_4:2467195-2467214 Msa0970810:intron 35.0%
! ACAAAAGTTTTCCAGAGGAT+TGG - chr6_4:2467196-2467215 Msa0970810:intron 35.0%
! ATCACACAAAAGTTTTCCAG+AGG - chr6_4:2467201-2467220 Msa0970810:intron 35.0%
ATCAACGACATTAAGACTTG+GGG + chr6_4:2467222-2467241 Msa0970820:intron 35.0%
! GGAGTTTTTCGCAAATTTGA+AGG - chr6_4:2467300-2467319 Msa0970810:intron 35.0%
! TCATCTGGTGTAACAAGTTT+TGG - chr6_4:2467760-2467779 Msa0970810:intron 35.0%
GCTACAGTTAGTGAGATATT+AGG + chr6_4:2467841-2467860 Msa0970820:CDS 35.0%
CAACACTATAAGTATCTCAG+CGG - chr6_4:2467947-2467966 Msa0970810:intron 35.0%
GATGTCTTTGTGTTTCCAAT+TGG + chr6_4:2464341-2464360 Msa0970820:CDS 35.0%
TGGAAGTGAATAAGACCAAT+TGG - chr6_4:2468060-2468079 Msa0970810:intron 35.0%
TTCACTTCCAACATAATGTG+GGG + chr6_4:2468070-2468089 Msa0970820:CDS 35.0%
! CTTCTGAAGATTGTCAACTA+TGG - chr6_4:2468226-2468245 Msa0970810:intron 35.0%
ACAATCTTCAGAAGCAATTC+TGG + chr6_4:2468232-2468251 Msa0970820:CDS 35.0%
ACATGATGCTAAAGCCAAGA+AGG - chr6_4:2463747-2463766 Msa0970810:intron 40.0%
! CAAAAGTCTTGTAGAGGACT+GGG - chr6_4:2463785-2463804 Msa0970810:intron 40.0%
! ACAAAAGTCTTGTAGAGGAC+TGG - chr6_4:2463786-2463805 Msa0970810:intron 40.0%
! TCCTCTACAAGACTTTTGTG+TGG + chr6_4:2463787-2463806 Msa0970820:CDS 40.0%
GCTATCAATGACACCAAGAA+TGG + chr6_4:2463809-2463828 Msa0970820:CDS 40.0%
ATCAATGACACCAAGAATGG+TGG + chr6_4:2463812-2463831 Msa0970820:CDS 40.0%
AGTTCAATCAGTTTAACGGG+TGG - chr6_4:2463901-2463920 Msa0970810:intron 40.0%
TATCAATGTTGTTGGACGTG+AGG - chr6_4:2463950-2463969 Msa0970810:intron 40.0%
TGTGAATGGCAAATTCTGCA+AGG + chr6_4:2464031-2464050 Msa0970820:CDS 40.0%
!! TCATTTGCTGTAGCAAGCTT+TGG - chr6_4:2464059-2464078 Msa0970810:intron 40.0%
! TTCTTCTACTCGGTGAAAGA+AGG + chr6_4:2464080-2464099 Msa0970820:CDS 40.0%
ACTTGGTTCAAAAGTGACAC+CGG + chr6_4:2464118-2464137 Msa0970820:CDS 40.0%
! TAAGAATCTCAGTGGCTCTA+GGG - chr6_4:2464238-2464257 Msa0970810:intron 40.0%
ATAAGAATCTCAGTGGCTCT+AGG - chr6_4:2464239-2464258 Msa0970810:intron 40.0%
CAACACGATAAGAATCTCAG+TGG - chr6_4:2464246-2464265 Msa0970810:intron 40.0%
CACTGAGATTCTTATCGTGT+TGG + chr6_4:2464244-2464263 Msa0970820:CDS 40.0%
TGAGATTCTTATCGTGTTGG+AGG + chr6_4:2464247-2464266 Msa0970820:CDS 40.0%
GAGATTCTTATCGTGTTGGA+GGG + chr6_4:2464248-2464267 Msa0970820:CDS 40.0%
!! TTGGAGGGTACTCTTTATGT+TGG + chr6_4:2464263-2464282 Msa0970820:intron 40.0%
!! TGGAGGGTACTCTTTATGTT+GGG + chr6_4:2464264-2464283 Msa0970820:intron 40.0%
! TTTGTGATGAGACGGTTTTC+TGG - chr6_4:2464305-2464324 Msa0970810:intron 40.0%
TGAAAGTGAATGAGGCCAAT+TGG - chr6_4:2464359-2464378 Msa0970810:intron 40.0%
!! TCTCACCAAAGCTTTTCAAG+TGG + chr6_4:2464496-2464515 Msa0970820:intron 40.0%
GACAACAACTAGAGAATTGC+AGG + chr6_4:2464560-2464579 Msa0970820:intron 40.0%
GAGTGAATGTGTGCAATTGA+TGG + chr6_4:2464900-2464919 Msa0970820:intron 40.0%
! CGTTTTTATACTTGTAGGGC+TGG + chr6_4:2465031-2465050 Msa0970820:intron 40.0%
AAAAACCAATCGCTGAGTCA+GGG + chr6_4:2465426-2465445 Msa0970820:intron 40.0%
ATAACGAAAGACTCTTCTGG+TGG + chr6_4:2465469-2465488 Msa0970820:intron 40.0%
!! AAAACTTGTTGATCTCTGGC+AGG + chr6_4:2465530-2465549 Msa0970820:intron 40.0%
TGTTATTGCATGAGATGGAG+GGG + chr6_4:2466642-2466661 Msa0970820:intron 40.0%
! AAGAGGGATTAGGGTTGATT+AGG + chr6_4:2466722-2466741 Msa0970820:intron 40.0%
! AGAGGGATTAGGGTTGATTA+GGG + chr6_4:2466723-2466742 Msa0970820:intron 40.0%
!!! GTAGATATTTTTTCGCGTGG+AGG - chr6_4:2466755-2466774 Msa0970810:intron 40.0%
TACATTTGATCCCAATCCTC+TGG + chr6_4:2467182-2467201 Msa0970820:intron 40.0%
GAAAACATATCACGTCCACT+TGG + chr6_4:2467802-2467821 Msa0970820:CDS 40.0%
!! TCGTATTGACTTTGCACCTA+AGG + chr6_4:2467891-2467910 Msa0970820:CDS 40.0%
!! CGTATTGACTTTGCACCTAA+GGG + chr6_4:2467892-2467911 Msa0970820:CDS 40.0%
!! TTGAAGGGTACTCTCAATGT+TGG + chr6_4:2467964-2467983 Msa0970820:CDS 40.0%
TCACTTCCAACATAATGTGG+GGG + chr6_4:2468071-2468090 Msa0970820:CDS 40.0%
TGAATTATGGCTGTTAAGCC+CGG - chr6_4:2468118-2468137 Msa0970810:intron 40.0%
TTCTTCAGAAGTTCTCACCA+AGG + chr6_4:2468182-2468201 Msa0970820:CDS 40.0%
TCTTCAGAAGCAATTCTGGT+TGG + chr6_4:2468236-2468255 Msa0970820:CDS 40.0%
CTTGTTTGTGACAGCCTTCT+TGG + chr6_4:2463730-2463749 Msa0970820:CDS 45.0%
TTGTAGAGGACTGGGATCAA+AGG - chr6_4:2463777-2463796 Msa0970810:intron 45.0%
! GCCACACAAAAGTCTTGTAG+AGG - chr6_4:2463791-2463810 Msa0970810:intron 45.0%
GGAAACGCATCAAATCCACT+TGG + chr6_4:2464101-2464120 Msa0970820:CDS 45.0%
!!! GGTGTCACTTTTGAACCAAG+TGG - chr6_4:2464119-2464138 Msa0970810:intron 45.0%
GGAAATGCAGTTGTCATTGC+TGG + chr6_4:2464395-2464414 Msa0970820:CDS 45.0%
! TGTGTGCAATTGATGGTCGT+AGG + chr6_4:2464907-2464926 Msa0970820:intron 45.0%
GAAAAACCAATCGCTGAGTC+AGG + chr6_4:2465425-2465444 Msa0970820:intron 45.0%
TGTGGAGGATTTAGACCCTT+AGG - chr6_4:2467910-2467929 Msa0970810:intron 45.0%
ATAAGTATCTCAGCGGCTCT+AGG - chr6_4:2467940-2467959 Msa0970810:intron 45.0%
TTCCAACATAATGTGGGGGA+TGG + chr6_4:2468075-2468094 Msa0970820:CDS 45.0%
TTCCATCCCCCACATTATGT+TGG - chr6_4:2468080-2468099 Msa0970810:intron 45.0%
ATTGTCCACTTGAAGAGCCT+TGG - chr6_4:2468202-2468221 Msa0970810:intron 45.0%
!!! AATTATAATCATATTTTTTT+TGG + chr6_4:2465992-2466011 Msa0970820:intron 5.0%
!!! AATTATAATCATATTTTTTT+TGG + chr6_4:2465992-2466011 Msa0970820:intron 5.0%
!!! AATTATAATCATATTTTTTT+TGG + chr6_4:2465992-2466011 Msa0970820:intron 5.0%
!!! AATTATAATCATATTTTTTT+TGG + chr6_4:2465992-2466011 Msa0970820:intron 5.0%
AGGACTGGGATCAAAGGCAA+AGG - chr6_4:2463771-2463790 Msa0970810:intron 50.0%
!! TCGCATTGACTTCGCTGCTA+GGG + chr6_4:2464190-2464209 Msa0970820:CDS 50.0%
GGACTTAGCAGCCAAAATCC+AGG + chr6_4:2464416-2464435 Msa0970820:CDS 50.0%
AGGCAGAACGTCGTGATTAG+AGG + chr6_4:2465278-2465297 Msa0970820:intron 50.0%
TGAAACCCTGACTCAGCGAT+TGG - chr6_4:2465434-2465453 Msa0970810:intron 50.0%
TCTAGGGAGGGATTGAGAGA+GGG - chr6_4:2466683-2466702 Msa0970810:intron 50.0%
ATCTAGGGAGGGATTGAGAG+AGG - chr6_4:2466684-2466703 Msa0970810:intron 50.0%
AGTGTCACATGCGAACCAAG+TGG - chr6_4:2467820-2467839 Msa0970810:intron 50.0%
! GGAAATGCAGCTGCCATTTC+CGG + chr6_4:2468096-2468115 Msa0970820:CDS 50.0%
! GAAATGCAGCTGCCATTTCC+GGG + chr6_4:2468097-2468116 Msa0970820:CDS 50.0%
ATGGCTGTTAAGCCCGGAAA+TGG - chr6_4:2468112-2468131 Msa0970810:intron 50.0%
TCTCACCAAGGCTCTTCAAG+TGG + chr6_4:2468194-2468213 Msa0970820:CDS 50.0%
!! CTCGCATTGACTTCGCTGCT+AGG + chr6_4:2464189-2464208 Msa0970820:CDS 55.0%
!! CGCATTGACTTCGCTGCTAG+GGG + chr6_4:2464191-2464210 Msa0970820:CDS 55.0%
!! GCTCTAGGGTGAGTATGTGG+AGG - chr6_4:2464224-2464243 Msa0970810:intron 55.0%
!! GTGGCTCTAGGGTGAGTATG+TGG - chr6_4:2464227-2464246 Msa0970810:intron 55.0%
AGACTCTTCTGGTGGCTCTC+TGG + chr6_4:2465477-2465496 Msa0970820:intron 55.0%
GATTGAGAGAGGGACACCCA+GGG - chr6_4:2466673-2466692 Msa0970810:intron 55.0%
!! GCTCTAGGATGAGCATGTGG+AGG - chr6_4:2467925-2467944 Msa0970810:intron 55.0%
AGAGAGGGACACCCAGGGTT+GGG - chr6_4:2466668-2466687 Msa0970810:intron 60.0%
GGATTGAGAGAGGGACACCC+AGG - chr6_4:2466674-2466693 Msa0970810:intron 60.0%
!! GCGGCTCTAGGATGAGCATG+TGG - chr6_4:2467928-2467947 Msa0970810:intron 60.0%
AGATGGAGGGGCCCAACCCT+GGG + chr6_4:2466654-2466673 Msa0970820:intron 65.0%
GAGAGAGGGACACCCAGGGT+TGG - chr6_4:2466669-2466688 Msa0970810:intron 65.0%
GAGATGGAGGGGCCCAACCC+TGG + chr6_4:2466653-2466672 Msa0970820:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 2463716 2468269 2463716 ID=Msa0970820;Name=Msa0970820
chr6_4 mRNA 2463716 2468269 2463716 ID=Msa0970820-mRNA-1;Parent=Msa0970820;Name=Msa0970820-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0|1|0.5|0.4|5|0|372
chr6_4 exon 2463716 2463833 2463716 ID=Msa0970820-mRNA-1:exon:1146;Parent=Msa0970820-mRNA-1
chr6_4 exon 2464027 2464269 2464027 ID=Msa0970820-mRNA-1:exon:1147;Parent=Msa0970820-mRNA-1
chr6_4 exon 2464306 2464514 2464306 ID=Msa0970820-mRNA-1:exon:1148;Parent=Msa0970820-mRNA-1
chr6_4 exon 2467237 2467243 2467237 ID=Msa0970820-mRNA-1:exon:1149;Parent=Msa0970820-mRNA-1
chr6_4 exon 2467728 2468269 2467728 ID=Msa0970820-mRNA-1:exon:1150;Parent=Msa0970820-mRNA-1
chr6_4 CDS 2463716 2463833 2463716 ID=Msa0970820-mRNA-1:cds;Parent=Msa0970820-mRNA-1
chr6_4 CDS 2464027 2464269 2464027 ID=Msa0970820-mRNA-1:cds;Parent=Msa0970820-mRNA-1
chr6_4 CDS 2464306 2464514 2464306 ID=Msa0970820-mRNA-1:cds;Parent=Msa0970820-mRNA-1
chr6_4 CDS 2467237 2467243 2467237 ID=Msa0970820-mRNA-1:cds;Parent=Msa0970820-mRNA-1
chr6_4 CDS 2467728 2468269 2467728 ID=Msa0970820-mRNA-1:cds;Parent=Msa0970820-mRNA-1
Gene Sequence

>Msa0970820

ATGAAGACTTCAATCTTGTTTGTGACAGCCTTCTTGGCTTTAGCATCATGTGCCTTTGCCTTTGATCCCAGTCCTCTACAAGACTTTTGTGTGGCTATCAATGACACCAAGAATGGTGTTTTTGTGAATGGCAAATTCTGCAAGGATCCAAAGCTTGCTACAGCAAATGATTTCTTCTACTCGGTGAAAGAAGGAAACGCATCAAATCCACTTGGTTCAAAAGTGACACCGGTTACCGTTAATGAGATACTAGGACTAAACACTTTAGGTATTTCTCTTGCTCGCATTGACTTCGCTGCTAGGGGACTAAATCCTCCACATACTCACCCTAGAGCCACTGAGATTCTTATCGTGTTGGAGGAAAACCGTCTCATCACAAAAGTACTCAACAAAGGAGATGTCTTTGTGTTTCCAATTGGCCTCATTCACTTTCAATTGAATGTTGGGTATGGAAATGCAGTTGTCATTGCTGGACTTAGCAGCCAAAATCCAGGTGTTATTACTATTGCTAATGCTGTATTTGGGTCTAATCCTAAAATCTCTTCAGAAGTTCTCACCAAAGCTTTTCAAACTTGGGTGTTGGTGAACGGAAAATGCTACAAAGATCCAAAACTTGTTACACCAGATGATTTCTTTTTCTCAGTAAAAGAAGAAAACATATCACGTCCACTTGGTTCGCATGTGACACTAGCTACAGTTAGTGAGATATTAGGATTAAATACTCTTGGTATCTCTTTTGCTCGTATTGACTTTGCACCTAAGGGTCTAAATCCTCCACATGCTCATCCTAGAGCCGCTGAGATACTTATAGTGTTGAAGGGTACTCTCAATGTTGGTTTTGTTACTTCTAACCCAAAAAACCGTCTCATTACAAAAGTTCTCAACAAAGGAGATGTCTTTGTGTTTCCAATTGGTCTTATTCACTTCCAACATAATGTGGGGGATGGAAATGCAGCTGCCATTTCCGGGCTTAACAGCCATAATTCAAGAGTTATCACTATTGTTAAGGAGGTATTTGGGTCTAATAAAATTTCTTCAGAAGTTCTCACCAAGGCTCTTCAAGTGGACAATACCATAGTTGACAATCTTCAGAAGCAATTCTGGTTGGACAACAATTAG
Protein sequence

>Msa0970820

MKTSILFVTAFLALASCAFAFDPSPLQDFCVAINDTKNGVFVNGKFCKDPKLATANDFFYSVKEGNASNPLGSKVTPVTVNEILGLNTLGISLARIDFAARGLNPPHTHPRATEILIVLEENRLITKVLNKGDVFVFPIGLIHFQLNVGYGNAVVIAGLSSQNPGVITIANAVFGSNPKISSEVLTKAFQTWVLVNGKCYKDPKLVTPDDFFFSVKEENISRPLGSHVTLATVSEILGLNTLGISFARIDFAPKGLNPPHAHPRAAEILIVLKGTLNVGFVTSNPKNRLITKVLNKGDVFVFPIGLIHFQHNVGDGNAAAISGLNSHNSRVITIVKEVFGSNKISSEVLTKALQVDNTIVDNLQKQFWLDNN*