AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0085710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085710 MtrunA17_Chr1g0201331 99.333 300 2 0 1 300 1 300 0.0 602
Msa0085710 MtrunA17_Chr4g0001221 59.375 288 102 2 9 287 7 288 2.67e-113 329
Msa0085710 MtrunA17_Chr2g0333771 56.908 304 111 6 13 300 11 310 3.57e-108 317
Msa0085710 MtrunA17_Chr4g0001111 54.839 217 83 2 9 216 7 217 3.13e-72 222
Msa0085710 MtrunA17_Chr8g0379911 49.356 233 107 3 71 300 35 259 3.44e-69 216
Msa0085710 MtrunA17_Chr8g0379901 49.362 235 108 3 69 300 34 260 4.12e-69 216
Msa0085710 MtrunA17_Chr2g0284581 47.600 250 112 4 54 300 26 259 2.21e-67 211
Msa0085710 MtrunA17_Chr2g0284631 50.673 223 108 2 62 283 80 301 3.34e-66 210
Msa0085710 MtrunA17_Chr2g0284591 48.718 234 109 3 70 300 32 257 6.87e-66 207
Msa0085710 MtrunA17_Chr2g0284611 48.696 230 113 2 74 300 38 265 6.39e-65 205
Msa0085710 MtrunA17_Chr7g0217331 44.749 219 121 0 72 290 35 253 1.68e-64 204
Msa0085710 MtrunA17_Chr2g0284601 49.057 212 97 3 92 300 8 211 2.22e-59 189
Msa0085710 MtrunA17_Chr5g0426131 39.706 272 137 5 28 294 6 255 6.68e-58 186
Msa0085710 MtrunA17_Chr4g0001161 63.571 140 51 0 87 226 1 140 3.55e-56 178
Msa0085710 MtrunA17_Chr1g0184541 43.111 225 128 0 69 293 57 281 1.44e-54 179
Msa0085710 MtrunA17_Chr5g0435051 39.921 253 138 3 32 283 10 249 4.84e-52 172
Msa0085710 MtrunA17_Chr4g0044201 42.373 236 110 5 70 300 31 245 1.27e-51 170
Msa0085710 MtrunA17_Chr3g0079421 41.004 239 135 4 47 283 1 235 4.85e-49 164
Msa0085710 MtrunA17_Chr4g0001151 60.305 131 37 1 87 217 1 116 8.02e-42 147
Msa0085710 MtrunA17_Chr4g0001121 64.835 91 24 2 216 300 10 98 3.13e-31 112
Msa0085710 MtrunA17_Chr2g0331261 36.481 233 128 6 69 287 15 241 4.19e-31 117
Msa0085710 MtrunA17_Chr1g0146161 35.841 226 126 7 74 287 19 237 2.71e-28 109
Msa0085710 MtrunA17_Chr8g0340161 37.089 213 113 7 78 275 23 229 9.17e-28 108
Msa0085710 MtrunA17_Chr1g0146171 35.981 214 118 7 74 275 19 225 1.59e-27 107
Msa0085710 MtrunA17_Chr1g0212671 33.333 228 132 7 78 291 25 246 1.02e-22 94.7
Msa0085710 MtrunA17_Chr5g0399341 33.333 234 134 5 78 298 23 247 2.73e-21 90.5
Msa0085710 MtrunA17_Chr2g0326621 30.677 251 139 7 71 287 37 286 2.43e-19 85.9
Msa0085710 MtrunA17_Chr4g0030861 32.870 216 123 8 79 279 26 234 6.75e-19 84.0
Msa0085710 MtrunA17_Chr7g0222791 31.330 233 130 8 78 291 25 246 1.86e-18 82.8
Msa0085710 MtrunA17_Chr6g0478191 28.571 224 149 4 78 293 25 245 6.05e-18 81.6
Msa0085710 MtrunA17_Chr7g0264961 30.804 224 144 6 78 292 25 246 6.89e-17 78.6
Msa0085710 MtrunA17_Chr1g0188771 30.357 224 145 5 78 293 25 245 9.19e-17 78.2
Msa0085710 MtrunA17_Chr3g0145671 29.060 234 148 7 66 282 29 261 3.90e-16 77.0
Msa0085710 MtrunA17_Chr4g0020781 32.967 182 102 5 126 299 36 205 2.24e-15 73.6
Msa0085710 MtrunA17_Chr8g0386961 29.646 226 139 8 78 285 56 279 1.68e-13 69.3
Msa0085710 MtrunA17_Chr4g0006001 29.333 225 140 9 78 285 60 282 1.73e-13 69.3
Msa0085710 MtrunA17_Chr7g0264111 27.092 251 157 8 71 299 36 282 1.87e-13 69.3
Msa0085710 MtrunA17_Chr3g0113211 26.126 222 147 6 78 282 59 280 3.25e-13 68.6
Msa0085710 MtrunA17_Chr5g0436701 26.667 225 147 6 78 285 59 282 6.98e-13 67.8
Msa0085710 MtrunA17_Chr5g0397391 28.319 226 142 8 78 285 56 279 8.15e-13 67.4
Msa0085710 MtrunA17_Chr4g0029071 25.591 254 157 7 66 287 31 284 2.11e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085710 AT4G10380.1 71.174 281 71 3 25 300 29 304 1.54e-143 406
Msa0085710 AT1G80760.1 56.550 313 101 4 9 300 7 305 6.30e-111 323
Msa0085710 AT5G37820.1 43.866 269 139 4 35 300 11 270 1.04e-68 215
Msa0085710 AT4G19030.1 49.167 240 108 3 69 300 50 283 1.40e-68 215
Msa0085710 AT5G37820.2 43.866 269 139 4 35 300 47 306 2.35e-68 216
Msa0085710 AT5G37810.1 42.435 271 140 4 35 300 11 270 1.51e-67 212
Msa0085710 AT4G18910.1 46.694 242 111 3 69 300 47 280 1.49e-65 207
Msa0085710 AT1G31885.1 50.673 223 102 2 69 283 38 260 5.27e-65 207
Msa0085710 AT1G31885.2 49.515 206 96 2 86 283 1 206 1.34e-57 186
Msa0085710 AT2G34390.1 43.777 233 117 4 69 291 43 271 2.14e-52 174
Msa0085710 AT2G34390.2 43.172 227 115 4 75 291 7 229 1.57e-50 167
Msa0085710 AT3G06100.1 44.762 210 113 3 78 285 50 258 3.20e-47 160
Msa0085710 AT3G06100.2 44.340 212 115 3 78 287 2 212 3.72e-47 158
Msa0085710 AT4G19030.3 49.143 175 81 2 69 235 50 224 3.19e-45 153
Msa0085710 AT4G18910.2 48.315 178 80 2 69 236 47 222 3.41e-44 150
Msa0085710 AT4G19030.2 46.795 156 75 2 69 216 50 205 1.55e-34 125
Msa0085710 AT2G34390.3 41.573 178 91 3 69 236 43 217 7.26e-32 118
Msa0085710 AT3G16240.1 37.500 208 110 7 78 271 23 224 1.07e-28 110
Msa0085710 AT2G29870.1 50.980 102 49 1 190 291 22 122 5.07e-26 100
Msa0085710 AT2G34390.4 40.625 160 82 3 69 218 43 199 2.46e-25 101
Msa0085710 AT5G47450.1 36.652 221 121 7 69 275 14 229 1.84e-24 99.8
Msa0085710 AT4G17340.1 35.849 212 117 6 78 275 23 229 5.16e-24 98.6
Msa0085710 AT2G25810.1 36.842 209 113 7 79 275 24 225 5.66e-24 98.2
Msa0085710 AT3G06100.3 39.855 138 82 1 149 285 40 177 2.73e-22 92.4
Msa0085710 AT3G26520.1 33.186 226 133 7 78 290 26 246 8.18e-22 92.4
Msa0085710 AT2G36830.1 34.348 230 129 8 78 292 25 247 2.10e-21 91.3
Msa0085710 AT4G01470.1 31.760 233 129 8 78 291 25 246 3.62e-19 85.1
Msa0085710 AT1G17810.1 29.258 229 148 5 78 293 28 255 2.03e-17 80.9
Msa0085710 AT1G31885.3 50.000 80 40 0 69 148 38 117 2.41e-17 77.4
Msa0085710 AT4G35100.1 29.600 250 156 7 66 299 30 275 8.17e-17 79.3
Msa0085710 AT4G35100.2 29.600 250 156 7 66 299 30 275 8.17e-17 79.3
Msa0085710 AT2G16850.1 29.796 245 152 7 71 299 33 273 1.02e-15 76.3
Msa0085710 AT1G73190.1 28.509 228 147 5 78 292 28 252 1.20e-15 75.9
Msa0085710 AT3G53420.1 27.376 263 164 8 59 297 24 283 8.11e-15 73.6
Msa0085710 AT3G53420.2 27.376 263 164 8 59 297 24 283 8.11e-15 73.6
Msa0085710 AT5G60660.1 26.383 235 147 7 72 282 37 269 3.46e-14 72.0
Msa0085710 AT2G37170.1 26.908 249 159 7 71 297 34 281 1.49e-13 70.1
Msa0085710 AT2G37170.2 26.908 249 159 7 71 297 77 324 2.44e-13 69.7
Msa0085710 AT3G54820.1 27.111 225 140 7 71 272 35 258 1.06e-12 67.4
Msa0085710 AT2G37180.1 26.104 249 161 7 71 297 34 281 2.71e-12 66.2
Msa0085710 AT2G45960.1 28.000 225 143 8 78 285 56 278 1.33e-11 64.3
Msa0085710 AT1G01620.1 27.488 211 137 6 78 272 56 266 1.61e-11 63.9
Msa0085710 AT3G61430.2 27.556 225 144 8 78 285 56 278 1.87e-11 63.9
Msa0085710 AT3G61430.1 27.556 225 144 8 78 285 56 278 1.87e-11 63.9
Msa0085710 AT3G47440.1 27.897 233 141 6 78 296 27 246 2.36e-11 63.2
Msa0085710 AT4G00430.1 27.232 224 146 7 78 285 57 279 2.77e-11 63.5
Msa0085710 AT4G23400.1 27.556 225 144 8 78 285 57 279 2.99e-11 63.2

Find 132 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCATCTCTGGATATATT+TGG 0.216287 1_2:+65998396 Msa0085710:CDS
AATCTCTCCAACTTCGAAAT+TGG 0.246759 1_2:+65993505 Msa0085710:five_prime_UTR
GGTGTCCCTGACTCCGATTC+TGG 0.274084 1_2:-65993888 None:intergenic
TTGTATTTGTTGTTCACTAT+TGG 0.275818 1_2:-65997338 None:intergenic
ATAGATTATAAAATTGAGTT+TGG 0.281086 1_2:-65993346 None:intergenic
AAAGAGTACAGTTACATAAT+TGG 0.297226 1_2:-65993715 None:intergenic
TTCGAAGTTGGAGAGATTAT+AGG 0.312909 1_2:-65993500 None:intergenic
CAATGGAGTAGAAACTCTTA+TGG 0.319532 1_2:+65997358 Msa0085710:CDS
CTTGGTGCTCTAGCTGGTTC+TGG 0.336743 1_2:+65998430 Msa0085710:CDS
ATAAATTTGTTTCTAAGGTA+TGG 0.361143 1_2:-65993683 None:intergenic
TTGCTCAACATTCTTATCTC+AGG 0.361959 1_2:+65997953 Msa0085710:CDS
GAACATGTGCCCACGGGAAA+TGG 0.378646 1_2:-65997490 None:intergenic
ATGAATCCGGTGCGCACCTT+AGG 0.389490 1_2:+65998349 Msa0085710:CDS
GTTTACCATCCTTTCCTCTC+TGG 0.391500 1_2:+65997572 Msa0085710:CDS
ATTCTTATAGATGAAAATAA+TGG 0.396106 1_2:-65993954 None:intergenic
TGCAATGCAGGTTGGTGAAT+TGG 0.400837 1_2:+65997904 Msa0085710:intron
TATGTAGTTGTTGTTAAGGT+TGG 0.402677 1_2:-65993565 None:intergenic
ATGTTCATTATTCTATCAAT+TGG 0.408502 1_2:+65997410 Msa0085710:CDS
ATCACTCACCATTGCCTTCG+CGG 0.410120 1_2:+65997460 Msa0085710:CDS
TAAGGTGCGCACCGGATTCA+TGG 0.410875 1_2:-65998347 None:intergenic
CAGGAAATTACAAGCATCTC+TGG 0.418374 1_2:+65998386 Msa0085710:CDS
TAATTTCCTGCTGCAACGGC+TGG 0.421426 1_2:-65998373 None:intergenic
CCGACGCTTGGTGCTCTAGC+TGG 0.422426 1_2:+65998424 Msa0085710:CDS
AAAATGCCAGAATCGGAGTC+AGG 0.425265 1_2:+65993882 Msa0085710:exon
TGCATCGCTAATTTCTCTGC+TGG 0.426344 1_2:+65994053 Msa0085710:CDS
TTTGGAAAGTGCTATGAATA+TGG 0.427152 1_2:-65993328 None:intergenic
AATGGCCCTCCGGGAGTATC+CGG 0.427525 1_2:-65993936 None:intergenic
TGGATATGTAGTTGTTGTTA+AGG 0.429772 1_2:-65993569 None:intergenic
TAATGAACATATGAAAAGAT+TGG 0.431700 1_2:-65993589 None:intergenic
CGGAGCTGAACCACCTCAAC+CGG 0.432157 1_2:+65998483 Msa0085710:CDS
TGCTGCAACGGCTGGACCTA+AGG 0.434795 1_2:-65998365 None:intergenic
TCAACAACTTATGGGGATGA+TGG 0.436919 1_2:-65993411 None:intergenic
AAGATGAGGTATTTAATTGT+GGG 0.439925 1_2:-65993622 None:intergenic
CAACGTGAGTCATTTGCAAT+GGG 0.443310 1_2:+65993990 Msa0085710:CDS
CGTTATCACGAAGCTTGATA+AGG 0.443454 1_2:-65998462 None:intergenic
TTCATGGAACCACCGCTTGT+TGG 0.443747 1_2:-65998331 None:intergenic
AAAGATGAGGTATTTAATTG+TGG 0.444642 1_2:-65993623 None:intergenic
CTATCAATTGGTCATATCTC+AGG 0.449732 1_2:+65997422 Msa0085710:CDS
TTGTGATCCAATTTCGAAGT+TGG 0.460953 1_2:-65993512 None:intergenic
CTTATCAAGCTTCGTGATAA+CGG 0.463299 1_2:+65998463 Msa0085710:CDS
GGAGCTTCCGTCGCTAGAGC+TGG 0.463603 1_2:+65998509 Msa0085710:exon
ATGGGAAATGCAGCTTGTGC+TGG 0.464614 1_2:+65997377 Msa0085710:CDS
TAAGAGAAAACTTGTGATGA+AGG 0.468893 1_2:-65993535 None:intergenic
AATGGAGTAGAAACTCTTAT+GGG 0.469616 1_2:+65997359 Msa0085710:CDS
TTTAGAAAATTATGTAGAGT+TGG 0.474450 1_2:+65993793 Msa0085710:five_prime_UTR
CTAAGGTATGGAAAGAAAGA+AGG 0.477970 1_2:-65993671 None:intergenic
GTTATCACGAAGCTTGATAA+GGG 0.481400 1_2:-65998461 None:intergenic
CTGAACCACCTCAACCGGTT+AGG 0.481691 1_2:+65998488 Msa0085710:CDS
TAGCGACGGAAGCTCCTAAC+CGG 0.489899 1_2:-65998502 None:intergenic
TGTGTCATGAAAGCTTTGTT+TGG 0.490520 1_2:+65998675 Msa0085710:three_prime_UTR
CTTGTAATTTCCTGCTGCAA+CGG 0.491803 1_2:-65998377 None:intergenic
AGCCTAATTAAATAAAATGT+GGG 0.492607 1_2:+65998594 Msa0085710:three_prime_UTR
CGGCGTTTCGCCATTTCCCG+TGG 0.493217 1_2:+65997480 Msa0085710:CDS
ACTTGTGCTGCAATGTAGGC+TGG 0.494118 1_2:-65997512 None:intergenic
GAGGTGCAACATGTGTATGC+CGG 0.494142 1_2:+65994013 Msa0085710:CDS
ACAGATTGGAGCAGAGTTTG+TGG 0.494648 1_2:+65997280 Msa0085710:intron
TATTTGGTGGCACCGACGCT+TGG 0.495981 1_2:+65998412 Msa0085710:CDS
TTAGGTCCAGCCGTTGCAGC+AGG 0.497485 1_2:+65998367 Msa0085710:CDS
CGGAAGCTCCTAACCGGTTG+AGG 0.497802 1_2:-65998496 None:intergenic
AAGCGGTGGTTCCATGAATC+CGG 0.497866 1_2:+65998336 Msa0085710:CDS
TACCATCCTTTCCTCTCTGG+TGG 0.500750 1_2:+65997575 Msa0085710:CDS
TCTAGCTAAATCTACAAACC+TGG 0.501272 1_2:+65993645 Msa0085710:five_prime_UTR
TTCACACACACTCGATTACA+AGG 0.504768 1_2:-65998621 None:intergenic
TGCTCAACATTCTTATCTCA+GGG 0.505305 1_2:+65997954 Msa0085710:CDS
AGATGAAAATAATGGCCCTC+CGG 0.507965 1_2:-65993946 None:intergenic
AGCGTCGGTGCCGGCGACAC+CGG 0.511138 1_2:+65993917 Msa0085710:CDS
AGGTGCAACATGTGTATGCC+GGG 0.512535 1_2:+65994014 Msa0085710:CDS
CAGATTGGAGCAGAGTTTGT+GGG 0.519331 1_2:+65997281 Msa0085710:intron
GCCATGGTTTGTATTATGAA+TGG 0.521934 1_2:+65998729 Msa0085710:three_prime_UTR
GAATTGGCTGGTATTGCTGT+TGG 0.524769 1_2:+65997920 Msa0085710:CDS
GATGAAAATAATGGCCCTCC+GGG 0.524874 1_2:-65993945 None:intergenic
AAAGCTTTGTTTGGTTAATG+TGG 0.526294 1_2:+65998684 Msa0085710:three_prime_UTR
TAATGGCAAGATTCTAGCCA+TGG 0.528152 1_2:+65998713 Msa0085710:three_prime_UTR
AAGTGTCTCTCTTTCTCAGA+AGG 0.536928 1_2:+65994088 Msa0085710:CDS
CCGGGAGTATCCGGTGTCGC+CGG 0.538656 1_2:-65993927 None:intergenic
AGAGACTTGTGCTGCAATGT+AGG 0.541162 1_2:-65997516 None:intergenic
AGTTGTTGTTAAGGTTGGAA+TGG 0.541398 1_2:-65993560 None:intergenic
ACCGACGGCAGCGTCGGTGC+CGG 0.542854 1_2:+65993908 Msa0085710:CDS
GAGCCTAATTAAATAAAATG+TGG 0.546465 1_2:+65998593 Msa0085710:three_prime_UTR
TCAACGTGAGTCATTTGCAA+TGG 0.548537 1_2:+65993989 Msa0085710:CDS
TGCGCTTGTTATGCGCTCAA+AGG 0.549531 1_2:+65997548 Msa0085710:CDS
TAGATTTAGCTAGAAAGATG+AGG 0.551425 1_2:-65993636 None:intergenic
AAGGTGGATGAGAGGACTAA+TGG 0.556098 1_2:-65993450 None:intergenic
AGTACAGTTACATAATTGGA+TGG 0.558005 1_2:-65993711 None:intergenic
GGCGTTTCGCCATTTCCCGT+GGG 0.558137 1_2:+65997481 Msa0085710:CDS
TAATAATGTGTTCTGTTGTG+TGG 0.558749 1_2:+65998765 Msa0085710:three_prime_UTR
GAGCTTGAAATATGAGAAGT+GGG 0.558919 1_2:-65998554 None:intergenic
GCGAAACGCCGCGAAGGCAA+TGG 0.561703 1_2:-65997468 None:intergenic
TTCTGAGAAAGAGAGACACT+TGG 0.567115 1_2:-65994086 None:intergenic
GCTTGTCCAACGCTAACAGT+AGG 0.567496 1_2:-65997608 None:intergenic
GCCGGCACCGACGCTGCCGT+CGG 0.571291 1_2:-65993909 None:intergenic
CTAATATATGCAGCAACAGC+AGG 0.571804 1_2:+65997314 Msa0085710:CDS
AAATGCCAGAATCGGAGTCA+GGG 0.577507 1_2:+65993883 Msa0085710:exon
CCGGCGACACCGGATACTCC+CGG 0.580871 1_2:+65993927 Msa0085710:CDS
TAAGGTATGGAAAGAAAGAA+GGG 0.581499 1_2:-65993670 None:intergenic
CTTGTCCAACGCTAACAGTA+GGG 0.582924 1_2:-65997607 None:intergenic
GCGAAGGCAATGGTGAGTGA+TGG 0.584999 1_2:-65997458 None:intergenic
AGAGCTTGAAATATGAGAAG+TGG 0.588128 1_2:-65998555 None:intergenic
GCATCTCTGGATATATTTGG+TGG 0.589329 1_2:+65998399 Msa0085710:CDS
CTAGCTAAATCTACAAACCT+GGG 0.589533 1_2:+65993646 Msa0085710:five_prime_UTR
GAAATGGCGAAACGCCGCGA+AGG 0.592464 1_2:-65997474 None:intergenic
ACCATTCATAATACAAACCA+TGG 0.595763 1_2:-65998730 None:intergenic
ATGCAGGTTGGTGAATTGGC+TGG 0.597369 1_2:+65997908 Msa0085710:intron
ATGAATTGAACGTACCGCAC+GGG 0.604011 1_2:-65997696 None:intergenic
CGTGGTTGAAGGTGGATGAG+AGG 0.608502 1_2:-65993458 None:intergenic
GCTGGACCTAAGGTGCGCAC+CGG 0.614709 1_2:-65998355 None:intergenic
TAGGCTGGAACATGTGCCCA+CGG 0.616186 1_2:-65997497 None:intergenic
ACTGTCCCTACTGTTAGCGT+TGG 0.619097 1_2:+65997602 Msa0085710:CDS
ATGATGGAGAAGAAGTGATG+AGG 0.621769 1_2:-65993395 None:intergenic
GTCGCAGCCAGCTCTAGCGA+CGG 0.626232 1_2:-65998516 None:intergenic
ATAGTGAACAACAAATACAA+TGG 0.626790 1_2:+65997341 Msa0085710:CDS
CCAGCTAGAGCACCAAGCGT+CGG 0.629619 1_2:-65998424 None:intergenic
AGGCTGGAACATGTGCCCAC+GGG 0.634760 1_2:-65997496 None:intergenic
CACCACCAGAGAGGAAAGGA+TGG 0.636860 1_2:-65997577 None:intergenic
AGGGACACCGACGGCAGCGT+CGG 0.638264 1_2:+65993902 Msa0085710:CDS
GCTTGAAATATGAGAAGTGG+GGG 0.639472 1_2:-65998552 None:intergenic
GTGAGTCATTTGCAATGGGG+AGG 0.644380 1_2:+65993994 Msa0085710:CDS
GCCTAATTAAATAAAATGTG+GGG 0.644829 1_2:+65998595 Msa0085710:three_prime_UTR
GTGACACCACCAGAGAGGAA+AGG 0.645575 1_2:-65997581 None:intergenic
TGGAAAGAAAGAAGGGACCC+AGG 0.648546 1_2:-65993663 None:intergenic
TGGATGAGAGGACTAATGGT+CGG 0.655282 1_2:-65993446 None:intergenic
AAGCTCCTAACCGGTTGAGG+TGG 0.655759 1_2:-65998493 None:intergenic
AATGCTAGGCCAACAAGCGG+TGG 0.661522 1_2:+65998322 Msa0085710:intron
AGCTTGAAATATGAGAAGTG+GGG 0.669139 1_2:-65998553 None:intergenic
ATCGGAGTCAGGGACACCGA+CGG 0.669278 1_2:+65993893 Msa0085710:CDS
GTAAATGCTAGGCCAACAAG+CGG 0.699062 1_2:+65998319 Msa0085710:intron
CGACACCGGATACTCCCGGA+GGG 0.703914 1_2:+65993931 Msa0085710:CDS
AACGTGAGTCATTTGCAATG+GGG 0.719998 1_2:+65993991 Msa0085710:CDS
GGACAGTGACACCACCAGAG+AGG 0.725765 1_2:-65997586 None:intergenic
GCGACACCGGATACTCCCGG+AGG 0.726349 1_2:+65993930 Msa0085710:CDS
TGCAACATGTGTATGCCGGG+TGG 0.746549 1_2:+65994017 Msa0085710:CDS
TGTTGCTACTGATTCCCGTG+CGG 0.778451 1_2:+65997682 Msa0085710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAAATTTGTTTCTA+AGG - chr1_2:65993691-65993710 None:intergenic 10.0%
!! TAGTTAATTTAATTAACAAT+TGG + chr1_2:65994395-65994414 Msa0085710:intron 10.0%
!! AGTTAATTTAATTAACAATT+GGG + chr1_2:65994396-65994415 Msa0085710:intron 10.0%
!!! AATGTATATTTTTTTTTTGA+AGG - chr1_2:65994683-65994702 None:intergenic 10.0%
!!! TAATAAAAAAATTGTTTTCA+AGG - chr1_2:65994769-65994788 None:intergenic 10.0%
!! AAGAAAAATGTATAAATAAA+GGG - chr1_2:65995105-65995124 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr1_2:65995106-65995125 None:intergenic 10.0%
!! TTATATTGAAATGAAATATA+TGG + chr1_2:65995224-65995243 Msa0085710:intron 10.0%
!!! ATTTCAATATATAATTTTGT+CGG + chr1_2:65995276-65995295 Msa0085710:intron 10.0%
!! TAAAATAAAAAAGATTAATG+CGG + chr1_2:65995665-65995684 Msa0085710:intron 10.0%
!! ATAGATTATAAAATTGAGTT+TGG - chr1_2:65993349-65993368 None:intergenic 15.0%
!! ATTCTTATAGATGAAAATAA+TGG - chr1_2:65993957-65993976 None:intergenic 15.0%
!!! AAATAGATAGCATTTTTTTT+AGG + chr1_2:65994302-65994321 Msa0085710:intron 15.0%
!!! AATAGATAGCATTTTTTTTA+GGG + chr1_2:65994303-65994322 Msa0085710:intron 15.0%
!!! ATTTTTGTTTGCTTTTTTTT+AGG - chr1_2:65994726-65994745 None:intergenic 15.0%
!!! GACTTTTTTTTTTAGAAAAT+GGG - chr1_2:65994971-65994990 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr1_2:65995040-65995059 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr1_2:65995041-65995060 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr1_2:65995104-65995123 None:intergenic 15.0%
!! AAAATTGTGTCAATTAAATT+GGG + chr1_2:65995141-65995160 Msa0085710:intron 15.0%
!! AAAACTATTCAATATACTTT+CGG - chr1_2:65995563-65995582 None:intergenic 15.0%
!!! TAAGCAAAAATTGATTTTTT+AGG + chr1_2:65995726-65995745 Msa0085710:intron 15.0%
!!! AAAAAAAAGTTGATTAAATG+AGG + chr1_2:65996202-65996221 Msa0085710:intron 15.0%
!!! AAAAAAAGTTGATTAAATGA+GGG + chr1_2:65996203-65996222 Msa0085710:intron 15.0%
!!! GAAATTACATAGATTTTTTT+TGG + chr1_2:65996708-65996727 Msa0085710:intron 15.0%
!!! TATAAGTTGTTTGTTTTTTT+TGG - chr1_2:65996838-65996857 None:intergenic 15.0%
!! ATTCAGACAAAATTTATATT+CGG - chr1_2:65998155-65998174 None:intergenic 15.0%
!! TAATGAACATATGAAAAGAT+TGG - chr1_2:65993592-65993611 None:intergenic 20.0%
!!! ATAAATTTGTTTCTAAGGTA+TGG - chr1_2:65993686-65993705 None:intergenic 20.0%
!!! TTTTGTTTTTGAGAGTTTTA+AGG - chr1_2:65993744-65993763 None:intergenic 20.0%
!! TTTAGAAAATTATGTAGAGT+TGG + chr1_2:65993793-65993812 Msa0085710:five_prime_UTR 20.0%
!! TTAAAAAAAAATGCCAGAAT+CGG + chr1_2:65993875-65993894 Msa0085710:exon 20.0%
!!! TCTATTTGATATTTTAGTCA+AGG - chr1_2:65994289-65994308 None:intergenic 20.0%
!! ATTACAAATACAAAAACAAG+TGG - chr1_2:65994427-65994446 None:intergenic 20.0%
!!! AGGAAATAAATTTTATTTGC+TGG - chr1_2:65994706-65994725 None:intergenic 20.0%
!! TACATATACTATATTACACA+CGG - chr1_2:65994802-65994821 None:intergenic 20.0%
!!! GGACTTTTTTTTTTAGAAAA+TGG - chr1_2:65994972-65994991 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+AGG - chr1_2:65995081-65995100 None:intergenic 20.0%
!! CAAAATTGTGTCAATTAAAT+TGG + chr1_2:65995140-65995159 Msa0085710:intron 20.0%
!!! AAATGTATTTTGACATAGAT+GGG + chr1_2:65995304-65995323 Msa0085710:intron 20.0%
!! TTTCATTGATAAACAAAAGA+TGG + chr1_2:65995344-65995363 Msa0085710:intron 20.0%
!!! ACATGTTTGATATTTTCTTA+GGG - chr1_2:65996163-65996182 None:intergenic 20.0%
!!! AACATGTTTGATATTTTCTT+AGG - chr1_2:65996164-65996183 None:intergenic 20.0%
!!! AAAAAAGTTGATTAAATGAG+GGG + chr1_2:65996204-65996223 Msa0085710:intron 20.0%
!! AAACTCAAAGTTTAAGTATT+CGG - chr1_2:65996254-65996273 None:intergenic 20.0%
!!! TTGGATTTATTTTTTTTGAG+AGG + chr1_2:65996343-65996362 Msa0085710:intron 20.0%
!! ATTGTTTAAACAACTAAAAG+TGG - chr1_2:65996602-65996621 None:intergenic 20.0%
!! ACAAAATTAAATCAACAACA+TGG - chr1_2:65996920-65996939 None:intergenic 20.0%
!!! TGATTTAATTTTGTAAGCTT+TGG + chr1_2:65996926-65996945 Msa0085710:intron 20.0%
!!! GATTTAATTTTGTAAGCTTT+GGG + chr1_2:65996927-65996946 Msa0085710:intron 20.0%
!!! ATTTAATTTTGTAAGCTTTG+GGG + chr1_2:65996928-65996947 Msa0085710:intron 20.0%
!!! AATGTATTTTTGAATACACA+TGG + chr1_2:65997055-65997074 Msa0085710:intron 20.0%
!!! TTTTTGGAAAAATCTACATT+TGG - chr1_2:65997087-65997106 None:intergenic 20.0%
!!! TTTTTCCAAAAATCTACATT+TGG + chr1_2:65997095-65997114 Msa0085710:intron 20.0%
!!! TACTTTTCCTTTTTCATTAA+GGG - chr1_2:65997225-65997244 None:intergenic 20.0%
!!! TTACTTTTCCTTTTTCATTA+AGG - chr1_2:65997226-65997245 None:intergenic 20.0%
!! ATGTTCATTATTCTATCAAT+TGG + chr1_2:65997410-65997429 Msa0085710:CDS 20.0%
!!! AAATTTCAAGTTCTCTAAAT+TGG + chr1_2:65997796-65997815 Msa0085710:intron 20.0%
!!! AATTTCAAGTTCTCTAAATT+GGG + chr1_2:65997797-65997816 Msa0085710:intron 20.0%
!!! TTGAGTTTTTTTATGAGTTA+AGG + chr1_2:65997849-65997868 Msa0085710:intron 20.0%
!!! TGATGTTATTGATTTGTATT+TGG + chr1_2:65997999-65998018 Msa0085710:intron 20.0%
!!! TTGTATTTGGTTTTCATAAT+AGG + chr1_2:65998012-65998031 Msa0085710:intron 20.0%
!!! ATTCAATGATAATGTTTCAA+AGG + chr1_2:65998065-65998084 Msa0085710:intron 20.0%
!! AGCCTAATTAAATAAAATGT+GGG + chr1_2:65998594-65998613 Msa0085710:three_prime_UTR 20.0%
!! TTGATTTTCAACAACTTATG+GGG - chr1_2:65993421-65993440 None:intergenic 25.0%
!!! GTTGATTTTCAACAACTTAT+GGG - chr1_2:65993422-65993441 None:intergenic 25.0%
!!! AGTTGATTTTCAACAACTTA+TGG - chr1_2:65993423-65993442 None:intergenic 25.0%
! AGAGATTATAGGAAAAAGAA+AGG - chr1_2:65993492-65993511 None:intergenic 25.0%
!! AAGATGAGGTATTTAATTGT+GGG - chr1_2:65993625-65993644 None:intergenic 25.0%
!! AAAGATGAGGTATTTAATTG+TGG - chr1_2:65993626-65993645 None:intergenic 25.0%
!! AAAGAGTACAGTTACATAAT+TGG - chr1_2:65993718-65993737 None:intergenic 25.0%
!! ATCTCTCTCTTTATGATTTT+TGG + chr1_2:65994115-65994134 Msa0085710:intron 25.0%
!!! ACCAAATGGAATTAATTTGT+GGG - chr1_2:65994193-65994212 None:intergenic 25.0%
!! AACCAAATGGAATTAATTTG+TGG - chr1_2:65994194-65994213 None:intergenic 25.0%
!!! TTTTTTTTGAAGGAATTTGC+TGG - chr1_2:65994673-65994692 None:intergenic 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - chr1_2:65994993-65995012 None:intergenic 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - chr1_2:65994994-65995013 None:intergenic 25.0%
! CTATGTCAAAATACATTTGA+AGG - chr1_2:65995302-65995321 None:intergenic 25.0%
!!! CAAATGTATTTTGACATAGA+TGG + chr1_2:65995303-65995322 Msa0085710:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr1_2:65995716-65995735 None:intergenic 25.0%
!! CTTTTACTTATAATAGTGAC+CGG + chr1_2:65995824-65995843 Msa0085710:intron 25.0%
!!! AGGAGGTATATATTTTACTA+AGG + chr1_2:65995900-65995919 Msa0085710:intron 25.0%
!!! GGAGGTATATATTTTACTAA+GGG + chr1_2:65995901-65995920 Msa0085710:intron 25.0%
!!! TATTTTTTTTGAGAGGAACT+TGG + chr1_2:65996350-65996369 Msa0085710:intron 25.0%
!!! ATTTTTTTTGAGAGGAACTT+GGG + chr1_2:65996351-65996370 Msa0085710:intron 25.0%
!!! TTTTTTTAAGGGAATGAACT+TGG + chr1_2:65996382-65996401 Msa0085710:intron 25.0%
!!! TTTTTTAAGGGAATGAACTT+GGG + chr1_2:65996383-65996402 Msa0085710:intron 25.0%
!! AAACAGTCTCAAAGTATTTA+TGG - chr1_2:65996783-65996802 None:intergenic 25.0%
!! ATGTTCCAAATGTAGATTTT+TGG - chr1_2:65997079-65997098 None:intergenic 25.0%
!!! ACTTTTCCTTTTTCATTAAG+GGG - chr1_2:65997224-65997243 None:intergenic 25.0%
!!! TTGTATTTGTTGTTCACTAT+TGG - chr1_2:65997341-65997360 None:intergenic 25.0%
! ATAGTGAACAACAAATACAA+TGG + chr1_2:65997341-65997360 Msa0085710:CDS 25.0%
!!! GTTAAGGTTGTTTTAATTCA+TGG + chr1_2:65997865-65997884 Msa0085710:intron 25.0%
!!! TATTTTTGTATATGCAATGC+AGG + chr1_2:65997892-65997911 Msa0085710:intron 25.0%
! TAACATCAATTCATGAGAAA+AGG - chr1_2:65997987-65998006 None:intergenic 25.0%
! TGTCAAATGTTATATTCAGT+TGG + chr1_2:65998041-65998060 Msa0085710:intron 25.0%
! ATCATGAAAAACTATTCCTA+AGG + chr1_2:65998093-65998112 Msa0085710:intron 25.0%
!! AATTTTGTCTGAATAATGAC+AGG + chr1_2:65998161-65998180 Msa0085710:intron 25.0%
! GAGCCTAATTAAATAAAATG+TGG + chr1_2:65998593-65998612 Msa0085710:three_prime_UTR 25.0%
! GCCTAATTAAATAAAATGTG+GGG + chr1_2:65998595-65998614 Msa0085710:three_prime_UTR 25.0%
!!! TCCCCACATTTTATTTAATT+AGG - chr1_2:65998599-65998618 None:intergenic 25.0%
!! TTTGGAAAGTGCTATGAATA+TGG - chr1_2:65993331-65993350 None:intergenic 30.0%
AAAAGAAAGGCAAAAAAACG+TGG - chr1_2:65993479-65993498 None:intergenic 30.0%
TAAGAGAAAACTTGTGATGA+AGG - chr1_2:65993538-65993557 None:intergenic 30.0%
! TATGTAGTTGTTGTTAAGGT+TGG - chr1_2:65993568-65993587 None:intergenic 30.0%
! TGGATATGTAGTTGTTGTTA+AGG - chr1_2:65993572-65993591 None:intergenic 30.0%
TAGATTTAGCTAGAAAGATG+AGG - chr1_2:65993639-65993658 None:intergenic 30.0%
TAAGGTATGGAAAGAAAGAA+GGG - chr1_2:65993673-65993692 None:intergenic 30.0%
AGTACAGTTACATAATTGGA+TGG - chr1_2:65993714-65993733 None:intergenic 30.0%
!! AAATGGAATTAATTTGTGGG+CGG - chr1_2:65994190-65994209 None:intergenic 30.0%
GTCAGATTTAACTGTTTCAT+GGG + chr1_2:65994227-65994246 Msa0085710:intron 30.0%
TAAATAGTATTGTTTGCACG+TGG + chr1_2:65994515-65994534 Msa0085710:intron 30.0%
TGTATAAATAAAGGGGAAAG+TGG - chr1_2:65995097-65995116 None:intergenic 30.0%
TTGTGTCAATTAAATTGGGA+CGG + chr1_2:65995145-65995164 Msa0085710:intron 30.0%
!!! TTTTGACATAGATGGGTTAT+TGG + chr1_2:65995311-65995330 Msa0085710:intron 30.0%
CTAGTAACATGGGTTTATTA+GGG - chr1_2:65995488-65995507 None:intergenic 30.0%
ACTAGTAACATGGGTTTATT+AGG - chr1_2:65995489-65995508 None:intergenic 30.0%
ACAAATGCAACTAGTAACAT+GGG - chr1_2:65995498-65995517 None:intergenic 30.0%
AACAAATGCAACTAGTAACA+TGG - chr1_2:65995499-65995518 None:intergenic 30.0%
TATATGACAGCCAAAATTTC+AGG + chr1_2:65995853-65995872 Msa0085710:intron 30.0%
ATATGACAGCCAAAATTTCA+GGG + chr1_2:65995854-65995873 Msa0085710:intron 30.0%
! GACATGTTTTCTCAATATCA+AGG + chr1_2:65995880-65995899 Msa0085710:intron 30.0%
! ATGTTTTCTCAATATCAAGG+AGG + chr1_2:65995883-65995902 Msa0085710:intron 30.0%
!! TATATTTTACTAAGGGTGTG+TGG + chr1_2:65995908-65995927 Msa0085710:intron 30.0%
TTTGGATTGAGAGTTTAAGA+GGG - chr1_2:65996136-65996155 None:intergenic 30.0%
! ATATTTTCTTAGGGTGTGTT+TGG - chr1_2:65996154-65996173 None:intergenic 30.0%
!!! TGGGTTCGACTTTTTTTTTA+AGG + chr1_2:65996370-65996389 Msa0085710:intron 30.0%
!!! GGGTTCGACTTTTTTTTTAA+GGG + chr1_2:65996371-65996390 Msa0085710:intron 30.0%
TTAGGTTAACAAGTGTCATA+AGG + chr1_2:65996478-65996497 Msa0085710:intron 30.0%
TAGGTTAACAAGTGTCATAA+GGG + chr1_2:65996479-65996498 Msa0085710:intron 30.0%
TTCAAAAGTTCTCCAAACAA+AGG + chr1_2:65996890-65996909 Msa0085710:intron 30.0%
GGATTAGAATAATGTGACAA+TGG - chr1_2:65997202-65997221 None:intergenic 30.0%
!! CTTTTCCTTTTTCATTAAGG+GGG - chr1_2:65997223-65997242 None:intergenic 30.0%
TGTTAAAATGTGTCACAGAT+TGG + chr1_2:65997266-65997285 Msa0085710:intron 30.0%
AATGGAGTAGAAACTCTTAT+GGG + chr1_2:65997359-65997378 Msa0085710:CDS 30.0%
TTAGAGAACTTGAAATTTCG+CGG - chr1_2:65997794-65997813 None:intergenic 30.0%
GTCATGTCATTAAAAACCTT+AGG - chr1_2:65998112-65998131 None:intergenic 30.0%
TAAGATTGTGAGTAATGTTG+TGG + chr1_2:65998294-65998313 Msa0085710:intron 30.0%
!! AAAGCTTTGTTTGGTTAATG+TGG + chr1_2:65998684-65998703 Msa0085710:three_prime_UTR 30.0%
! GGTTAATGTGGATTTTCTAA+TGG + chr1_2:65998696-65998715 Msa0085710:three_prime_UTR 30.0%
ACCATTCATAATACAAACCA+TGG - chr1_2:65998733-65998752 None:intergenic 30.0%
TTCGAAGTTGGAGAGATTAT+AGG - chr1_2:65993503-65993522 None:intergenic 35.0%
AATCTCTCCAACTTCGAAAT+TGG + chr1_2:65993505-65993524 Msa0085710:five_prime_UTR 35.0%
TTGTGATCCAATTTCGAAGT+TGG - chr1_2:65993515-65993534 None:intergenic 35.0%
! AGTTGTTGTTAAGGTTGGAA+TGG - chr1_2:65993563-65993582 None:intergenic 35.0%
TCTAGCTAAATCTACAAACC+TGG + chr1_2:65993645-65993664 Msa0085710:five_prime_UTR 35.0%
CTAGCTAAATCTACAAACCT+GGG + chr1_2:65993646-65993665 Msa0085710:five_prime_UTR 35.0%
CTAAGGTATGGAAAGAAAGA+AGG - chr1_2:65993674-65993693 None:intergenic 35.0%
!!! GATTTTTGGTCAACGAGTTT+TGG + chr1_2:65994129-65994148 Msa0085710:intron 35.0%
GCCCACAAATTAATTCCATT+TGG + chr1_2:65994189-65994208 Msa0085710:intron 35.0%
GGTCAGATTTAACTGTTTCA+TGG + chr1_2:65994226-65994245 Msa0085710:intron 35.0%
AGAAAATGGGTCAGTTATTC+CGG - chr1_2:65994958-65994977 None:intergenic 35.0%
GAAACAGTGCAAAAACATGT+AGG + chr1_2:65995393-65995412 Msa0085710:intron 35.0%
TGTAGGATATTAAGCTACAC+TGG + chr1_2:65995410-65995429 Msa0085710:intron 35.0%
! TTAAGCTACACTGGTTATCT+TGG + chr1_2:65995419-65995438 Msa0085710:intron 35.0%
AGTTTAAGAGGGAAAAGGAA+GGG - chr1_2:65996125-65996144 None:intergenic 35.0%
TTGAGAGTTTAAGAGGGAAA+AGG - chr1_2:65996130-65996149 None:intergenic 35.0%
GTTTGGATTGAGAGTTTAAG+AGG - chr1_2:65996137-65996156 None:intergenic 35.0%
! AAGTTGATTAAATGAGGGGT+AGG + chr1_2:65996208-65996227 Msa0085710:intron 35.0%
ATCGTGTCAATCTGAAAACT+TGG + chr1_2:65996318-65996337 Msa0085710:intron 35.0%
TCGTGTCAATCTGAAAACTT+GGG + chr1_2:65996319-65996338 Msa0085710:intron 35.0%
TCAATCTGAAAACTTGGGTT+TGG + chr1_2:65996324-65996343 Msa0085710:intron 35.0%
!! TGTCATAAGGGCATTGTTTA+AGG + chr1_2:65996491-65996510 Msa0085710:intron 35.0%
CAACATGGTTAACCTTTGTT+TGG - chr1_2:65996905-65996924 None:intergenic 35.0%
! GAATGATGATGTGTGTACAT+AGG + chr1_2:65996954-65996973 Msa0085710:intron 35.0%
AGCATTGCTTGAGTTATTAG+TGG + chr1_2:65997005-65997024 Msa0085710:intron 35.0%
!! AGAGCTACTTTGGAGTTTAT+TGG - chr1_2:65997127-65997146 None:intergenic 35.0%
GTAGAATGAAAGAGCTACTT+TGG - chr1_2:65997137-65997156 None:intergenic 35.0%
CTAATCCCCCTTAATGAAAA+AGG + chr1_2:65997215-65997234 Msa0085710:intron 35.0%
CAATGGAGTAGAAACTCTTA+TGG + chr1_2:65997358-65997377 Msa0085710:CDS 35.0%
CTATCAATTGGTCATATCTC+AGG + chr1_2:65997422-65997441 Msa0085710:CDS 35.0%
TTTGTATATGCAATGCAGGT+TGG + chr1_2:65997896-65997915 Msa0085710:intron 35.0%
TTGCTCAACATTCTTATCTC+AGG + chr1_2:65997953-65997972 Msa0085710:CDS 35.0%
TGCTCAACATTCTTATCTCA+GGG + chr1_2:65997954-65997973 Msa0085710:CDS 35.0%
AACATTCTTATCTCAGGGTA+TGG + chr1_2:65997959-65997978 Msa0085710:intron 35.0%
ATATTCGGAGACTAACTTGA+TGG - chr1_2:65998140-65998159 None:intergenic 35.0%
ATGTTGTGGTTGTAAATGCT+AGG + chr1_2:65998308-65998327 Msa0085710:intron 35.0%
CAAGCATCTCTGGATATATT+TGG + chr1_2:65998396-65998415 Msa0085710:CDS 35.0%
GTTATCACGAAGCTTGATAA+GGG - chr1_2:65998464-65998483 None:intergenic 35.0%
CTTATCAAGCTTCGTGATAA+CGG + chr1_2:65998463-65998482 Msa0085710:CDS 35.0%
AGCTTGAAATATGAGAAGTG+GGG - chr1_2:65998556-65998575 None:intergenic 35.0%
GAGCTTGAAATATGAGAAGT+GGG - chr1_2:65998557-65998576 None:intergenic 35.0%
AGAGCTTGAAATATGAGAAG+TGG - chr1_2:65998558-65998577 None:intergenic 35.0%
!! TGTGTCATGAAAGCTTTGTT+TGG + chr1_2:65998675-65998694 Msa0085710:three_prime_UTR 35.0%
! GCCATGGTTTGTATTATGAA+TGG + chr1_2:65998729-65998748 Msa0085710:three_prime_UTR 35.0%
! ATGATGGAGAAGAAGTGATG+AGG - chr1_2:65993398-65993417 None:intergenic 40.0%
TCAACAACTTATGGGGATGA+TGG - chr1_2:65993414-65993433 None:intergenic 40.0%
CAAAAAAACGTGGTTGAAGG+TGG - chr1_2:65993469-65993488 None:intergenic 40.0%
AGGCAAAAAAACGTGGTTGA+AGG - chr1_2:65993472-65993491 None:intergenic 40.0%
AGATGAAAATAATGGCCCTC+CGG - chr1_2:65993949-65993968 None:intergenic 40.0%
TCAACGTGAGTCATTTGCAA+TGG + chr1_2:65993989-65994008 Msa0085710:CDS 40.0%
CAACGTGAGTCATTTGCAAT+GGG + chr1_2:65993990-65994009 Msa0085710:CDS 40.0%
AACGTGAGTCATTTGCAATG+GGG + chr1_2:65993991-65994010 Msa0085710:CDS 40.0%
!! CAACTATTGCTTTTACCACC+CGG - chr1_2:65994035-65994054 None:intergenic 40.0%
! TTCTGAGAAAGAGAGACACT+TGG - chr1_2:65994089-65994108 None:intergenic 40.0%
AAGTGTCTCTCTTTCTCAGA+AGG + chr1_2:65994088-65994107 Msa0085710:CDS 40.0%
CATGCAAGCTAGAAACCAAA+TGG - chr1_2:65994207-65994226 None:intergenic 40.0%
!! CATTTGGTTTCTAGCTTGCA+TGG + chr1_2:65994205-65994224 Msa0085710:intron 40.0%
TGTGATGAGATGTGCATGAT+TGG + chr1_2:65994472-65994491 Msa0085710:intron 40.0%
GTGATGAGATGTGCATGATT+GGG + chr1_2:65994473-65994492 Msa0085710:intron 40.0%
GAAAATGGGTCAGTTATTCC+GGG - chr1_2:65994957-65994976 None:intergenic 40.0%
TGTCAATTAAATTGGGACGG+AGG + chr1_2:65995148-65995167 Msa0085710:intron 40.0%
! GGGAGTACGAGTTAAGTAAA+TGG - chr1_2:65995692-65995711 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr1_2:65995712-65995731 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr1_2:65995713-65995732 None:intergenic 40.0%
TGGCTGTCATATACTTCATC+CGG - chr1_2:65995846-65995865 None:intergenic 40.0%
!! TTTTACTAAGGGTGTGTGGA+TGG + chr1_2:65995912-65995931 Msa0085710:intron 40.0%
ATGGAGAGTTTAAGAGAGGA+TGG + chr1_2:65995931-65995950 Msa0085710:intron 40.0%
TGGAGAGTTTAAGAGAGGAT+GGG + chr1_2:65995932-65995951 Msa0085710:intron 40.0%
GTTTAAGAGGGAAAAGGAAG+GGG - chr1_2:65996124-65996143 None:intergenic 40.0%
GAGTTTAAGAGGGAAAAGGA+AGG - chr1_2:65996126-65996145 None:intergenic 40.0%
CTAATATATGCAGCAACAGC+AGG + chr1_2:65997314-65997333 Msa0085710:CDS 40.0%
AATGAATTGAACGTACCGCA+CGG - chr1_2:65997700-65997719 None:intergenic 40.0%
!! TTTCGCGGCAACTTTTTAAG+TGG - chr1_2:65997779-65997798 None:intergenic 40.0%
GTCTGAATAATGACAGGATC+AGG + chr1_2:65998167-65998186 Msa0085710:intron 40.0%
CTTGTAATTTCCTGCTGCAA+CGG - chr1_2:65998380-65998399 None:intergenic 40.0%
CAGGAAATTACAAGCATCTC+TGG + chr1_2:65998386-65998405 Msa0085710:CDS 40.0%
GCATCTCTGGATATATTTGG+TGG + chr1_2:65998399-65998418 Msa0085710:CDS 40.0%
CGTTATCACGAAGCTTGATA+AGG - chr1_2:65998465-65998484 None:intergenic 40.0%
GCTTGAAATATGAGAAGTGG+GGG - chr1_2:65998555-65998574 None:intergenic 40.0%
TTCACACACACTCGATTACA+AGG - chr1_2:65998624-65998643 None:intergenic 40.0%
TAATGGCAAGATTCTAGCCA+TGG + chr1_2:65998713-65998732 Msa0085710:three_prime_UTR 40.0%
TGGATGAGAGGACTAATGGT+CGG - chr1_2:65993449-65993468 None:intergenic 45.0%
AAGGTGGATGAGAGGACTAA+TGG - chr1_2:65993453-65993472 None:intergenic 45.0%
AAAATGCCAGAATCGGAGTC+AGG + chr1_2:65993882-65993901 Msa0085710:exon 45.0%
AAATGCCAGAATCGGAGTCA+GGG + chr1_2:65993883-65993902 Msa0085710:exon 45.0%
GATGAAAATAATGGCCCTCC+GGG - chr1_2:65993948-65993967 None:intergenic 45.0%
TGCATCGCTAATTTCTCTGC+TGG + chr1_2:65994053-65994072 Msa0085710:CDS 45.0%
!! TGGAATTAATTTGTGGGCGG+TGG - chr1_2:65994187-65994206 None:intergenic 45.0%
TACTTAACTCGTACTCCCTC+CGG + chr1_2:65995694-65995713 Msa0085710:intron 45.0%
GACAGCCAAAATTTCAGGGA+CGG + chr1_2:65995858-65995877 Msa0085710:intron 45.0%
! CATGTCCGTCCCTGAAATTT+TGG - chr1_2:65995866-65995885 None:intergenic 45.0%
GTGGATGGAGAGTTTAAGAG+AGG + chr1_2:65995927-65995946 Msa0085710:intron 45.0%
AGAGTTTAAGAGAGGATGGG+AGG + chr1_2:65995935-65995954 Msa0085710:intron 45.0%
GAGTTTAAGAGAGGATGGGA+GGG + chr1_2:65995936-65995955 Msa0085710:intron 45.0%
AGTTTAAGAGAGGATGGGAG+GGG + chr1_2:65995937-65995956 Msa0085710:intron 45.0%
ACAGATTGGAGCAGAGTTTG+TGG + chr1_2:65997280-65997299 Msa0085710:intron 45.0%
CAGATTGGAGCAGAGTTTGT+GGG + chr1_2:65997281-65997300 Msa0085710:intron 45.0%
AGAGACTTGTGCTGCAATGT+AGG - chr1_2:65997519-65997538 None:intergenic 45.0%
GTTTACCATCCTTTCCTCTC+TGG + chr1_2:65997572-65997591 Msa0085710:CDS 45.0%
CTTGTCCAACGCTAACAGTA+GGG - chr1_2:65997610-65997629 None:intergenic 45.0%
ATGAATTGAACGTACCGCAC+GGG - chr1_2:65997699-65997718 None:intergenic 45.0%
!! TGCAATGCAGGTTGGTGAAT+TGG + chr1_2:65997904-65997923 Msa0085710:intron 45.0%
GAATTGGCTGGTATTGCTGT+TGG + chr1_2:65997920-65997939 Msa0085710:CDS 45.0%
GTAAATGCTAGGCCAACAAG+CGG + chr1_2:65998319-65998338 Msa0085710:intron 45.0%
!! AAAATTAAAAAAAAAAAGTT+AGG + chr1_2:65996460-65996479 Msa0085710:intron 5.0%
!!! TTTATTTTACATTTATTTTT+AGG - chr1_2:65996650-65996669 None:intergenic 5.0%
TGGAAAGAAAGAAGGGACCC+AGG - chr1_2:65993666-65993685 None:intergenic 50.0%
GTGAGTCATTTGCAATGGGG+AGG + chr1_2:65993994-65994013 Msa0085710:CDS 50.0%
GAGGTGCAACATGTGTATGC+CGG + chr1_2:65994013-65994032 Msa0085710:CDS 50.0%
AGGTGCAACATGTGTATGCC+GGG + chr1_2:65994014-65994033 Msa0085710:CDS 50.0%
ATGGGTCAGTTATTCCGGGA+CGG - chr1_2:65994953-65994972 None:intergenic 50.0%
ATGGGAAATGCAGCTTGTGC+TGG + chr1_2:65997377-65997396 Msa0085710:CDS 50.0%
ATCACTCACCATTGCCTTCG+CGG + chr1_2:65997460-65997479 Msa0085710:CDS 50.0%
ACTTGTGCTGCAATGTAGGC+TGG - chr1_2:65997515-65997534 None:intergenic 50.0%
!! TGCGCTTGTTATGCGCTCAA+AGG + chr1_2:65997548-65997567 Msa0085710:CDS 50.0%
TACCATCCTTTCCTCTCTGG+TGG + chr1_2:65997575-65997594 Msa0085710:CDS 50.0%
ACTGTCCCTACTGTTAGCGT+TGG + chr1_2:65997602-65997621 Msa0085710:CDS 50.0%
GCTTGTCCAACGCTAACAGT+AGG - chr1_2:65997611-65997630 None:intergenic 50.0%
!! TGTTGCTACTGATTCCCGTG+CGG + chr1_2:65997682-65997701 Msa0085710:CDS 50.0%
!! ATGCAGGTTGGTGAATTGGC+TGG + chr1_2:65997908-65997927 Msa0085710:intron 50.0%
TTCATGGAACCACCGCTTGT+TGG - chr1_2:65998334-65998353 None:intergenic 50.0%
AAGCGGTGGTTCCATGAATC+CGG + chr1_2:65998336-65998355 Msa0085710:CDS 50.0%
TAATTTCCTGCTGCAACGGC+TGG - chr1_2:65998376-65998395 None:intergenic 50.0%
CGTGGTTGAAGGTGGATGAG+AGG - chr1_2:65993461-65993480 None:intergenic 55.0%
TGCAACATGTGTATGCCGGG+TGG + chr1_2:65994017-65994036 Msa0085710:CDS 55.0%
TAATTGCACTCCCTCCGTCC+CGG + chr1_2:65994936-65994955 Msa0085710:intron 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr1_2:65994949-65994968 None:intergenic 55.0%
GCGAAGGCAATGGTGAGTGA+TGG - chr1_2:65997461-65997480 None:intergenic 55.0%
GAACATGTGCCCACGGGAAA+TGG - chr1_2:65997493-65997512 None:intergenic 55.0%
TAGGCTGGAACATGTGCCCA+CGG - chr1_2:65997500-65997519 None:intergenic 55.0%
CACCACCAGAGAGGAAAGGA+TGG - chr1_2:65997580-65997599 None:intergenic 55.0%
GTGACACCACCAGAGAGGAA+AGG - chr1_2:65997584-65997603 None:intergenic 55.0%
AATGCTAGGCCAACAAGCGG+TGG + chr1_2:65998322-65998341 Msa0085710:intron 55.0%
TAAGGTGCGCACCGGATTCA+TGG - chr1_2:65998350-65998369 None:intergenic 55.0%
ATGAATCCGGTGCGCACCTT+AGG + chr1_2:65998349-65998368 Msa0085710:CDS 55.0%
!! TATTTGGTGGCACCGACGCT+TGG + chr1_2:65998412-65998431 Msa0085710:CDS 55.0%
!! CTTGGTGCTCTAGCTGGTTC+TGG + chr1_2:65998430-65998449 Msa0085710:CDS 55.0%
CTGAACCACCTCAACCGGTT+AGG + chr1_2:65998488-65998507 Msa0085710:CDS 55.0%
AAGCTCCTAACCGGTTGAGG+TGG - chr1_2:65998496-65998515 None:intergenic 55.0%
TAGCGACGGAAGCTCCTAAC+CGG - chr1_2:65998505-65998524 None:intergenic 55.0%
GGTGTCCCTGACTCCGATTC+TGG - chr1_2:65993891-65993910 None:intergenic 60.0%
ATCGGAGTCAGGGACACCGA+CGG + chr1_2:65993893-65993912 Msa0085710:CDS 60.0%
!! AATGGCCCTCCGGGAGTATC+CGG - chr1_2:65993939-65993958 None:intergenic 60.0%
GGTCAGTTATTCCGGGACGG+AGG - chr1_2:65994950-65994969 None:intergenic 60.0%
GAAATGGCGAAACGCCGCGA+AGG - chr1_2:65997477-65997496 None:intergenic 60.0%
GGCGTTTCGCCATTTCCCGT+GGG + chr1_2:65997481-65997500 Msa0085710:CDS 60.0%
AGGCTGGAACATGTGCCCAC+GGG - chr1_2:65997499-65997518 None:intergenic 60.0%
GGACAGTGACACCACCAGAG+AGG - chr1_2:65997589-65997608 None:intergenic 60.0%
TGCTGCAACGGCTGGACCTA+AGG - chr1_2:65998368-65998387 None:intergenic 60.0%
TTAGGTCCAGCCGTTGCAGC+AGG + chr1_2:65998367-65998386 Msa0085710:CDS 60.0%
! CCAGCTAGAGCACCAAGCGT+CGG - chr1_2:65998427-65998446 None:intergenic 60.0%
CGGAGCTGAACCACCTCAAC+CGG + chr1_2:65998483-65998502 Msa0085710:CDS 60.0%
CGGAAGCTCCTAACCGGTTG+AGG - chr1_2:65998499-65998518 None:intergenic 60.0%
!! AAGTGGGGGTGTCGAGAGCA+GGG - chr1_2:65998541-65998560 None:intergenic 60.0%
CGACACCGGATACTCCCGGA+GGG + chr1_2:65993931-65993950 Msa0085710:CDS 65.0%
GCGAAACGCCGCGAAGGCAA+TGG - chr1_2:65997471-65997490 None:intergenic 65.0%
CGGCGTTTCGCCATTTCCCG+TGG + chr1_2:65997480-65997499 Msa0085710:CDS 65.0%
GCTGGACCTAAGGTGCGCAC+CGG - chr1_2:65998358-65998377 None:intergenic 65.0%
!! CCGACGCTTGGTGCTCTAGC+TGG + chr1_2:65998424-65998443 Msa0085710:CDS 65.0%
GGAGCTTCCGTCGCTAGAGC+TGG + chr1_2:65998509-65998528 Msa0085710:exon 65.0%
! GTCGCAGCCAGCTCTAGCGA+CGG - chr1_2:65998519-65998538 None:intergenic 65.0%
!! GAAGTGGGGGTGTCGAGAGC+AGG - chr1_2:65998542-65998561 None:intergenic 65.0%
AGGGACACCGACGGCAGCGT+CGG + chr1_2:65993902-65993921 Msa0085710:CDS 70.0%
!! CCGGGAGTATCCGGTGTCGC+CGG - chr1_2:65993930-65993949 None:intergenic 70.0%
CCGGCGACACCGGATACTCC+CGG + chr1_2:65993927-65993946 Msa0085710:CDS 70.0%
GCGACACCGGATACTCCCGG+AGG + chr1_2:65993930-65993949 Msa0085710:CDS 70.0%
!! ACCGACGGCAGCGTCGGTGC+CGG + chr1_2:65993908-65993927 Msa0085710:CDS 75.0%
!! AGCGTCGGTGCCGGCGACAC+CGG + chr1_2:65993917-65993936 Msa0085710:CDS 75.0%
!!! GCCGGCACCGACGCTGCCGT+CGG - chr1_2:65993912-65993931 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 65993295 65998787 65993295 ID=Msa0085710;Name=Msa0085710
chr1_2 mRNA 65993295 65998787 65993295 ID=Msa0085710-mRNA-1;Parent=Msa0085710;Name=Msa0085710-mRNA-1;_AED=0.03;_eAED=0.03;_QI=590|1|1|1|1|1|4|262|300
chr1_2 exon 65993295 65994109 65993295 ID=Msa0085710-mRNA-1:exon:22305;Parent=Msa0085710-mRNA-1
chr1_2 exon 65997284 65997703 65997284 ID=Msa0085710-mRNA-1:exon:22306;Parent=Msa0085710-mRNA-1
chr1_2 exon 65997914 65997975 65997914 ID=Msa0085710-mRNA-1:exon:22307;Parent=Msa0085710-mRNA-1
chr1_2 exon 65998330 65998787 65998330 ID=Msa0085710-mRNA-1:exon:22308;Parent=Msa0085710-mRNA-1
chr1_2 five_prime_UTR 65993295 65993884 65993295 ID=Msa0085710-mRNA-1:five_prime_utr;Parent=Msa0085710-mRNA-1
chr1_2 CDS 65993885 65994109 65993885 ID=Msa0085710-mRNA-1:cds;Parent=Msa0085710-mRNA-1
chr1_2 CDS 65997284 65997703 65997284 ID=Msa0085710-mRNA-1:cds;Parent=Msa0085710-mRNA-1
chr1_2 CDS 65997914 65997975 65997914 ID=Msa0085710-mRNA-1:cds;Parent=Msa0085710-mRNA-1
chr1_2 CDS 65998330 65998525 65998330 ID=Msa0085710-mRNA-1:cds;Parent=Msa0085710-mRNA-1
chr1_2 three_prime_UTR 65998526 65998787 65998526 ID=Msa0085710-mRNA-1:three_prime_utr;Parent=Msa0085710-mRNA-1
Gene Sequence

>Msa0085710

ATGCCAGAATCGGAGTCAGGGACACCGACGGCAGCGTCGGTGCCGGCGACACCGGATACTCCCGGAGGGCCATTATTTTCATCTATAAGAATTGATTCACTTGATCAACGTGAGTCATTTGCAATGGGGAGGTGCAACATGTGTATGCCGGGTGGTAAAAGCAATAGTTGCATCGCTAATTTCTCTGCTGGAGTTCGTATTCCAAGTGTCTCTCTTTCTCAGAAGATTGGAGCAGAGTTTGTGGGAACATTCATACTAATATATGCAGCAACAGCAGGACCAATAGTGAACAACAAATACAATGGAGTAGAAACTCTTATGGGAAATGCAGCTTGTGCTGGACTAACAGTCATGTTCATTATTCTATCAATTGGTCATATCTCAGGTGCACATCTCAATCCATCACTCACCATTGCCTTCGCGGCGTTTCGCCATTTCCCGTGGGCACATGTTCCAGCCTACATTGCAGCACAAGTCTCTGCTTCGATTTGCGCTTGTTATGCGCTCAAAGGAGTTTACCATCCTTTCCTCTCTGGTGGTGTCACTGTCCCTACTGTTAGCGTTGGACAAGCTTTTGCAACTGAGTTTATCATCACTTTTATTCTCTTGTTTGTTGTTACTGCTGTTGCTACTGATTCCCGTGCGGTTGGTGAATTGGCTGGTATTGCTGTTGGTGCTACAGTTTTGCTCAACATTCTTATCTCAGGGCCAACAAGCGGTGGTTCCATGAATCCGGTGCGCACCTTAGGTCCAGCCGTTGCAGCAGGAAATTACAAGCATCTCTGGATATATTTGGTGGCACCGACGCTTGGTGCTCTAGCTGGTTCTGGAGTTTATACCCTTATCAAGCTTCGTGATAACGGAGCTGAACCACCTCAACCGGTTAGGAGCTTCCGTCGCTAG
Protein sequence

>Msa0085710

MPESESGTPTAASVPATPDTPGGPLFSSIRIDSLDQRESFAMGRCNMCMPGGKSNSCIANFSAGVRIPSVSLSQKIGAEFVGTFILIYAATAGPIVNNKYNGVETLMGNAACAGLTVMFIILSIGHISGAHLNPSLTIAFAAFRHFPWAHVPAYIAAQVSASICACYALKGVYHPFLSGGVTVPTVSVGQAFATEFIITFILLFVVTAVATDSRAVGELAGIAVGATVLLNILISGPTSGGSMNPVRTLGPAVAAGNYKHLWIYLVAPTLGALAGSGVYTLIKLRDNGAEPPQPVRSFRR*