AlfalfaGEDB | Msa1441300

M. sativa cultivar zhongmu-4 / Msa1441300

KEGG: K00430
GO: GO:0000902 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004601 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009636 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016209 GO:0016491 GO:0016684 GO:0030312 GO:0031090 GO:0031347 GO:0031349 GO:0032502 GO:0032989 GO:0035821 GO:0040007 GO:0040008 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045926 GO:0048046 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0055114 GO:0060560 GO:0065007 GO:0070887 GO:0071840 GO:0071944 GO:0072593 GO:0075136 GO:0080134 GO:0097237 GO:0098542 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1990748
PFAM: PF00141.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1441300	sp\|P17180\|PER3_ARMRU	59.547	309	124	1	25	332	27	335	1.52e-134	390

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1441300	A4UN77	94.648	355	18	1	1	355	1	354	0.0	685

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa1441300	0.806578	7.685978e-50	-8.615850e-47
Msa0021870	Msa1441300	0.803931	2.754397e-49	-8.615850e-47
Msa0024650	Msa1441300	0.817852	2.659421e-52	-8.615850e-47
Msa0048380	Msa1441300	0.801855	7.393671e-49	-8.615850e-47
Msa0059880	Msa1441300	0.826153	3.170659e-54	-8.615850e-47
Msa0061600	Msa1441300	0.800931	1.143405e-48	-8.615850e-47
Msa0085710	Msa1441300	0.805137	1.543354e-49	-8.615850e-47
Msa0093530	Msa1441300	0.801923	7.159231e-49	-8.615850e-47
Msa0120450	Msa1441300	0.804642	1.958158e-49	-8.615850e-47
Msa0125600	Msa1441300	0.813571	2.393358e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1441300	MtrunA17_Chr2g0292151	94.648	355	18	1	1	355	1	354	0.0	685
Msa1441300	MtrunA17_Chr2g0292111	86.479	355	48	0	1	355	1	355	0.0	632
Msa1441300	MtrunA17_Chr2g0291751	76.901	355	82	0	1	355	1	355	0.0	565
Msa1441300	MtrunA17_Chr2g0292181	73.803	355	91	1	1	355	10	362	0.0	535
Msa1441300	MtrunA17_Chr2g0292211	72.676	355	94	2	1	355	12	363	0.0	520
Msa1441300	MtrunA17_Chr2g0292241	70.141	355	106	0	1	355	1	355	0.0	514
Msa1441300	MtrunA17_Chr2g0292251	69.553	358	103	3	1	355	1	355	2.16e-175	491
Msa1441300	MtrunA17_Chr2g0292271	66.292	356	116	3	1	355	1	353	2.09e-165	466
Msa1441300	MtrunA17_Chr2g0292261	65.254	354	120	1	1	354	1	351	2.80e-163	460
Msa1441300	MtrunA17_Chr2g0292291	63.944	355	123	2	2	355	3	353	1.11e-160	454

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1441300	AT2G38380.1	59.223	309	125	1	25	332	27	335	1.02e-134	388
Msa1441300	AT2G38390.1	57.605	309	130	1	25	332	27	335	5.55e-132	381
Msa1441300	AT5G06720.1	54.574	317	144	0	14	330	17	333	2.98e-124	361
Msa1441300	AT4G08780.1	54.430	316	142	2	25	338	20	335	4.31e-122	356
Msa1441300	AT4G08770.1	54.688	320	143	2	25	342	20	339	1.78e-121	354
Msa1441300	AT5G06730.1	50.915	328	161	0	14	341	18	345	2.09e-120	352
Msa1441300	AT3G32980.1	53.153	333	147	3	25	355	27	352	1.82e-119	349
Msa1441300	AT3G49120.1	52.553	333	149	3	25	355	28	353	2.18e-117	344
Msa1441300	AT3G49110.1	52.553	333	149	3	25	355	29	354	2.64e-116	341
Msa1441300	AT5G19880.1	51.250	320	148	5	15	330	14	329	4.23e-110	325

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Find 93 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGTTGACAATGCTAA+TGG	0.353968	tig0029281:-7628	None:intergenic
AAATACAATAGATTGCAATT+GGG	0.425537	tig0029281:-5974	None:intergenic
AAATCTGCCTTTGCTGATCA+AGG	0.421581	tig0029281:+6667	Msa1441300:CDS
AAATTGACAACGTTATTTCC+AGG	0.383901	tig0029281:-7495	None:intergenic
AACCTCAATATCATATAGCT+TGG	0.505657	tig0029281:+5831	Msa1441300:five_prime_UTR
AACGTGCTCTTCCAAATGTA+TGG	0.396511	tig0029281:-7362	None:intergenic
AACTACTACAACAATCTTCA+AGG	0.429163	tig0029281:+7549	Msa1441300:CDS
AAGTATGTTGGGTCAAGAGT+TGG	0.500202	tig0029281:-7438	None:intergenic
AAGTTGATCTAAACTGAAGT+TGG	0.456582	tig0029281:-6644	None:intergenic
AAGTTGTGCATTTGAGGAGA+AGG	0.531405	tig0029281:-5923	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCTCAATATCATATAGCT+TGG	+	tig0029281:5831-5850	Msa1441300:five_prime_UTR	30.0%
TACCAAGCTATATGATATTG+AGG	-	tig0029281:5836-5855	None:intergenic	30.0%
CTTCTTAAGGAGTTTACATT+TGG	-	tig0029281:6313-6332	None:intergenic	30.0%
TGTAAACTCCTTAAGAAGAT+TGG	+	tig0029281:6315-6334	Msa1441300:CDS	30.0%
CAATCAGATTAAGACTAAAG+TGG	+	tig0029281:6345-6364	Msa1441300:CDS	30.0%
ATCTATGTATATTCACAGAG+TGG	+	tig0029281:6538-6557	Msa1441300:intron	30.0%
TCTATGTATATTCACAGAGT+GGG	+	tig0029281:6539-6558	Msa1441300:intron	30.0%
AGTTGATCTAAACTGAAGTT+GGG	-	tig0029281:6646-6665	None:intergenic	30.0%
AAAGTAAGTGAACTTTGATG+AGG	+	tig0029281:7200-7219	Msa1441300:intron	30.0%
AACTACTACAACAATCTTCA+AGG	+	tig0029281:7549-7568	Msa1441300:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0029281	gene	5761	8010	5761	ID=Msa1441300;Name=Msa1441300
tig0029281	mRNA	5761	8010	5761	ID=Msa1441300-mRNA-1;Parent=Msa1441300;Name=Msa1441300-mRNA-1;_AED=0.08;_eAED=0.08;_QI=98\|1\|1\|1\|1\|1\|4\|165\|355
tig0029281	exon	5761	6080	5761	ID=Msa1441300-mRNA-1:exon:12738;Parent=Msa1441300-mRNA-1
tig0029281	exon	6247	6438	6247	ID=Msa1441300-mRNA-1:exon:12739;Parent=Msa1441300-mRNA-1
tig0029281	exon	6556	6721	6556	ID=Msa1441300-mRNA-1:exon:12740;Parent=Msa1441300-mRNA-1
tig0029281	exon	7358	8010	7358	ID=Msa1441300-mRNA-1:exon:12741;Parent=Msa1441300-mRNA-1
tig0029281	five_prime_UTR	5761	5858	5761	ID=Msa1441300-mRNA-1:five_prime_utr;Parent=Msa1441300-mRNA-1
tig0029281	CDS	5859	6080	5859	ID=Msa1441300-mRNA-1:cds;Parent=Msa1441300-mRNA-1
tig0029281	CDS	6247	6438	6247	ID=Msa1441300-mRNA-1:cds;Parent=Msa1441300-mRNA-1
tig0029281	CDS	6556	6721	6556	ID=Msa1441300-mRNA-1:cds;Parent=Msa1441300-mRNA-1
tig0029281	CDS	7358	7845	7358	ID=Msa1441300-mRNA-1:cds;Parent=Msa1441300-mRNA-1
tig0029281	three_prime_UTR	7846	8010	7846	ID=Msa1441300-mRNA-1:three_prime_utr;Parent=Msa1441300-mRNA-1

Gene Sequence

>Msa1441300

ATGAACTCCCTAAGTCTCATAGCAACAGCTCTATGCTGTGTAGTAGTTGTGTTTGGAGGACTACCCTTCTCCTCAAATGCACAACTTCGTCCAGACTTTTACGCCAAAACATGTCCCCAATTGCAATCTATTGTATTTCAAATCTTAGAGAAGGTTTCTAAGACTGATTCTCGAATGCCTGCGAGTATCATCAGGCTTCACTTTCATGATTGTTTCGTTCAAGGTTGTGATGCATCAGTTTTGTTGAATAACACTGATACTATTAAGAGTGAACAAGATGCTGGTCCAAATGTAAACTCCTTAAGAAGATTGGACGTTATCAATCAGATTAAGACTAAAGTGGAAAAAGTTTGTCCTAATACAGTTTCTTGTGCTGATATTCTCACACTTGCAGCTGGAGTATCTTCAGTTCTGAGTGGGGGTCCAGGTTGGATCGTTCCATTGGGAAGAAGGGATAGTCTAACAGCAAGTCAATCCCTTGCCAATCAAAATCTTCCAGGTCCCAACTTCAGTTTAGATCAACTTAAATCTGCCTTTGCTGATCAAGGACTCAACACGGTTGATCTTGTAGCACTCTCAGGTGCCCATACATTTGGAAGAGCACGTTGCCTTTTCATACTTGATCGATTATACGACTTCGACAATACTGGCAAACCTGATCCAACTCTTGACCCAACATACTTAAAACAGTTGCAAAAACAATGTCCTCAGAATGGACCTGGAAATAACGTTGTCAATTTTGACCCAACAACTCCCGATAAATTAGACAAGAACTACTACAACAATCTTCAAGGTAAAAAGGGTTTGCTTCAAAGTGACCAAGAATTGTTCTCAACACCAGGTGCAGATACCATTAGCATTGTCAACAACTTTGGAAATAACCAAAATGTTTTCTTCCAGAACTTTATAAATTCAATGATTAAAATGGGTAATATTGGCGTGCTTACCGGAACGAAAGGAGAAATTAGAAAACAATGCAATTTTGTTAACAAGAAATCTTCTGAATTGGATCTTGCCACTGTGGCCTCTACAGAATCAATGGAAGGGGATATGGTTAGTTCAATCTAA

Protein sequence

>Msa1441300

MNSLSLIATALCCVVVVFGGLPFSSNAQLRPDFYAKTCPQLQSIVFQILEKVSKTDSRMPASIIRLHFHDCFVQGCDASVLLNNTDTIKSEQDAGPNVNSLRRLDVINQIKTKVEKVCPNTVSCADILTLAAGVSSVLSGGPGWIVPLGRRDSLTASQSLANQNLPGPNFSLDQLKSAFADQGLNTVDLVALSGAHTFGRARCLFILDRLYDFDNTGKPDPTLDPTYLKQLQKQCPQNGPGNNVVNFDPTTPDKLDKNYYNNLQGKKGLLQSDQELFSTPGADTISIVNNFGNNQNVFFQNFINSMIKMGNIGVLTGTKGEIRKQCNFVNKKSSELDLATVASTESMEGDMVSSI*