Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1181680 | sp|P82787|DEF19_ARATH | 51.351 | 74 | 33 | 1 | 9 | 79 | 5 | 78 | 7.12e-21 | 80.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1181680 | A0A072TL50 | 94.937 | 79 | 4 | 0 | 1 | 79 | 1 | 79 | 1.80e-47 | 154 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0027860 | Msa1181680 | 0.807370 | 5.226481e-50 | -8.615850e-47 |
| Msa0059880 | Msa1181680 | 0.816748 | 4.711608e-52 | -8.615850e-47 |
| Msa0605470 | Msa1181680 | 0.806441 | 8.215693e-50 | -8.615850e-47 |
| Msa0631700 | Msa1181680 | 0.805052 | 1.607868e-49 | -8.615850e-47 |
| Msa0634290 | Msa1181680 | 0.822575 | 2.201272e-53 | -8.615850e-47 |
| Msa0651820 | Msa1181680 | 0.821252 | 4.457867e-53 | -8.615850e-47 |
| Msa0652610 | Msa1181680 | 0.802098 | 6.591060e-49 | -8.615850e-47 |
| Msa0668220 | Msa1181680 | 0.806373 | 8.489296e-50 | -8.615850e-47 |
| Msa0670360 | Msa1181680 | 0.808277 | 3.352661e-50 | -8.615850e-47 |
| Msa0671490 | Msa1181680 | 0.833194 | 6.136164e-56 | -8.615850e-47 |
| Msa0685080 | Msa1181680 | 0.800126 | 1.668091e-48 | -8.615850e-47 |
| Msa0701490 | Msa1181680 | 0.804247 | 2.367716e-49 | -8.615850e-47 |
| Msa0701560 | Msa1181680 | 0.804409 | 2.189927e-49 | -8.615850e-47 |
| Msa0711230 | Msa1181680 | 0.800021 | 1.752084e-48 | -8.615850e-47 |
| Msa0711240 | Msa1181680 | 0.816957 | 4.229926e-52 | -8.615850e-47 |
| Msa0727480 | Msa1181680 | 0.818837 | 1.591429e-52 | -8.615850e-47 |
| Msa0728520 | Msa1181680 | 0.806382 | 8.451174e-50 | -8.615850e-47 |
| Msa0768670 | Msa1181680 | 0.808675 | 2.756355e-50 | -8.615850e-47 |
| Msa0768720 | Msa1181680 | 0.806384 | 8.443004e-50 | -8.615850e-47 |
| Msa0776540 | Msa1181680 | 0.802980 | 4.335810e-49 | -8.615850e-47 |
| Msa0792330 | Msa1181680 | 0.803938 | 2.745236e-49 | -8.615850e-47 |
| Msa0814350 | Msa1181680 | 0.800535 | 1.376830e-48 | -8.615850e-47 |
| Msa0129850 | Msa1181680 | 0.810386 | 1.182974e-50 | -8.615850e-47 |
| Msa0143640 | Msa1181680 | 0.814007 | 1.918241e-51 | -8.615850e-47 |
| Msa0150910 | Msa1181680 | 0.814052 | 1.874798e-51 | -8.615850e-47 |
| Msa0207640 | Msa1181680 | 0.807887 | 4.058273e-50 | -8.615850e-47 |
| Msa0375030 | Msa1181680 | 0.806752 | 7.062152e-50 | -8.615850e-47 |
| Msa0409490 | Msa1181680 | 0.807539 | 4.810867e-50 | -8.615850e-47 |
| Msa0414480 | Msa1181680 | 0.802921 | 4.458913e-49 | -8.615850e-47 |
| Msa0421080 | Msa1181680 | 0.803463 | 3.444260e-49 | -8.615850e-47 |
| Msa0464300 | Msa1181680 | 0.803036 | 4.222309e-49 | -8.615850e-47 |
| Msa0464310 | Msa1181680 | 0.812947 | 3.280064e-51 | -8.615850e-47 |
| Msa0470440 | Msa1181680 | 0.806321 | 8.707298e-50 | -8.615850e-47 |
| Msa1006170 | Msa1181680 | 0.803730 | 3.031859e-49 | -8.615850e-47 |
| Msa1023430 | Msa1181680 | 0.807350 | 5.275734e-50 | -8.615850e-47 |
| Msa1024350 | Msa1181680 | 0.820137 | 8.041468e-53 | -8.615850e-47 |
| Msa1026050 | Msa1181680 | 0.847779 | 9.316789e-60 | -8.615850e-47 |
| Msa1048350 | Msa1181680 | 0.807009 | 6.230767e-50 | -8.615850e-47 |
| Msa1061670 | Msa1181680 | 0.808937 | 2.422705e-50 | -8.615850e-47 |
| Msa1061840 | Msa1181680 | 0.812799 | 3.534156e-51 | -8.615850e-47 |
| Msa1064940 | Msa1181680 | 0.816288 | 5.973191e-52 | -8.615850e-47 |
| Msa1076830 | Msa1181680 | 0.807897 | 4.037824e-50 | -8.615850e-47 |
| Msa1087380 | Msa1181680 | 0.823178 | 1.593147e-53 | -8.615850e-47 |
| Msa1106960 | Msa1181680 | 0.800121 | 1.671851e-48 | -8.615850e-47 |
| Msa0259110 | Msa1181680 | 0.801256 | 9.810840e-49 | -8.615850e-47 |
| Msa0265060 | Msa1181680 | 0.800582 | 1.347044e-48 | -8.615850e-47 |
| Msa0272210 | Msa1181680 | 0.800232 | 1.587226e-48 | -8.615850e-47 |
| Msa0274260 | Msa1181680 | 0.821356 | 4.217126e-53 | -8.615850e-47 |
| Msa0278460 | Msa1181680 | 0.825403 | 4.778086e-54 | -8.615850e-47 |
| Msa0342130 | Msa1181680 | 0.801867 | 7.352791e-49 | -8.615850e-47 |
| Msa0346170 | Msa1181680 | 0.831104 | 2.016948e-55 | -8.615850e-47 |
| Msa1107510 | Msa1181680 | 0.802946 | 4.407204e-49 | -8.615850e-47 |
| Msa1109880 | Msa1181680 | 0.825084 | 5.685412e-54 | -8.615850e-47 |
| Msa1109890 | Msa1181680 | 0.813247 | 2.818610e-51 | -8.615850e-47 |
| Msa1138110 | Msa1181680 | 0.813579 | 2.382883e-51 | -8.615850e-47 |
| Msa1150760 | Msa1181680 | 0.802521 | 5.392862e-49 | -8.615850e-47 |
| Msa1151070 | Msa1181680 | 0.802447 | 5.585857e-49 | -8.615850e-47 |
| Msa1162430 | Msa1181680 | 0.809704 | 1.659135e-50 | -8.615850e-47 |
| Msa1181680 | Msa1211610 | 0.801240 | 9.882942e-49 | -8.615850e-47 |
| Msa1181680 | Msa1214960 | 0.824787 | 6.680867e-54 | -8.615850e-47 |
| Msa1181680 | Msa1300570 | 0.809421 | 1.908459e-50 | -8.615850e-47 |
| Msa1181680 | Msa1320160 | 0.811709 | 6.112513e-51 | -8.615850e-47 |
| Msa1181680 | Msa1323120 | 0.803181 | 3.940358e-49 | -8.615850e-47 |
| Msa1181680 | Msa1355330 | 0.813505 | 2.474761e-51 | -8.615850e-47 |
| Msa1181680 | Msa1355340 | 0.806554 | 7.775752e-50 | -8.615850e-47 |
| Msa1181680 | Msa1373560 | 0.820897 | 5.380473e-53 | -8.615850e-47 |
| Msa1181680 | Msa1376070 | 0.808022 | 3.799240e-50 | -8.615850e-47 |
| Msa1181680 | Msa1411550 | 0.802252 | 6.128025e-49 | -8.615850e-47 |
| Msa1181680 | Msa1419210 | 0.801193 | 1.010775e-48 | -8.615850e-47 |
| Msa1181680 | Msa1421130 | 0.801163 | 1.024851e-48 | -8.615850e-47 |
| Msa1181680 | Msa1456600 | 0.813516 | 2.460764e-51 | -8.615850e-47 |
| Msa0489370 | Msa1181680 | 0.811463 | 6.912549e-51 | -8.615850e-47 |
| Msa0497680 | Msa1181680 | 0.803143 | 4.012443e-49 | -8.615850e-47 |
| Msa0515130 | Msa1181680 | 0.804059 | 2.590047e-49 | -8.615850e-47 |
| Msa0529420 | Msa1181680 | 0.802425 | 5.644787e-49 | -8.615850e-47 |
| Msa0535570 | Msa1181680 | 0.811984 | 5.326604e-51 | -8.615850e-47 |
| Msa0538440 | Msa1181680 | 0.828307 | 9.669434e-55 | -8.615850e-47 |
| Msa0538450 | Msa1181680 | 0.824379 | 8.335427e-54 | -8.615850e-47 |
| Msa0538520 | Msa1181680 | 0.826763 | 2.269293e-54 | -8.615850e-47 |
| Msa0538530 | Msa1181680 | 0.823043 | 1.712791e-53 | -8.615850e-47 |
| Msa0554660 | Msa1181680 | 0.805899 | 1.067974e-49 | -8.615850e-47 |
| Msa0580840 | Msa1181680 | 0.805193 | 1.502529e-49 | -8.615850e-47 |
| Msa0589740 | Msa1181680 | 0.809839 | 1.551740e-50 | -8.615850e-47 |
| Msa0591040 | Msa1181680 | 0.803248 | 3.816524e-49 | -8.615850e-47 |
| Msa0591490 | Msa1181680 | 0.800142 | 1.655809e-48 | -8.615850e-47 |
| Msa0594320 | Msa1181680 | 0.814093 | 1.835843e-51 | -8.615850e-47 |
| Msa0594330 | Msa1181680 | 0.808144 | 3.577631e-50 | -8.615850e-47 |
| Msa0599600 | Msa1181680 | 0.801256 | 9.808653e-49 | -8.615850e-47 |
| Msa0855260 | Msa1181680 | 0.822453 | 2.349354e-53 | -8.615850e-47 |
| Msa0871420 | Msa1181680 | 0.803009 | 4.277454e-49 | -8.615850e-47 |
| Msa0874750 | Msa1181680 | 0.808903 | 2.463383e-50 | -8.615850e-47 |
| Msa0886370 | Msa1181680 | 0.803670 | 3.120789e-49 | -8.615850e-47 |
| Msa0896920 | Msa1181680 | 0.841189 | 5.526679e-58 | -8.615850e-47 |
| Msa0902340 | Msa1181680 | 0.831472 | 1.636933e-55 | -8.615850e-47 |
| Msa0910830 | Msa1181680 | 0.801078 | 1.067042e-48 | -8.615850e-47 |
| Msa0932910 | Msa1181680 | 0.813761 | 2.173067e-51 | -8.615850e-47 |
| Msa0932930 | Msa1181680 | 0.807428 | 5.080391e-50 | -8.615850e-47 |
| Msa0933580 | Msa1181680 | 0.800265 | 1.563163e-48 | -8.615850e-47 |
| Msa0933590 | Msa1181680 | 0.810464 | 1.137716e-50 | -8.615850e-47 |
| Msa0933610 | Msa1181680 | 0.824531 | 7.672926e-54 | -8.615850e-47 |
| Msa0962290 | Msa1181680 | 0.811918 | 5.504519e-51 | -8.615850e-47 |
| Msa0976400 | Msa1181680 | 0.813941 | 1.983242e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1181680 | MtrunA17_Chr8g0339701 | 94.937 | 79 | 4 | 0 | 1 | 79 | 1 | 79 | 3.46e-51 | 154 |
| Msa1181680 | MtrunA17_Chr8g0339681 | 88.608 | 79 | 9 | 0 | 1 | 79 | 1 | 79 | 2.15e-48 | 149 |
| Msa1181680 | MtrunA17_Chr8g0339681 | 64.706 | 68 | 24 | 0 | 12 | 79 | 69 | 136 | 2.03e-27 | 96.7 |
| Msa1181680 | MtrunA17_Chr8g0339721 | 67.089 | 79 | 26 | 0 | 1 | 79 | 1 | 79 | 1.30e-28 | 97.8 |
| Msa1181680 | MtrunA17_Chr8g0339691 | 78.431 | 51 | 11 | 0 | 27 | 77 | 1 | 51 | 1.44e-26 | 93.2 |
| Msa1181680 | MtrunA17_Chr8g0339741 | 55.000 | 80 | 35 | 1 | 1 | 79 | 1 | 80 | 1.04e-24 | 87.8 |
| Msa1181680 | MtrunA17_Chr8g0339711 | 51.250 | 80 | 37 | 2 | 1 | 79 | 1 | 79 | 1.69e-21 | 79.7 |
| Msa1181680 | MtrunA17_Chr8g0339751 | 56.962 | 79 | 33 | 1 | 1 | 79 | 1 | 78 | 5.69e-20 | 75.9 |
| Msa1181680 | MtrunA17_Chr3g0084701 | 51.899 | 79 | 36 | 1 | 1 | 79 | 1 | 77 | 1.79e-19 | 74.7 |
| Msa1181680 | MtrunA17_Chr8g0339651 | 46.835 | 79 | 37 | 2 | 4 | 79 | 13 | 89 | 2.68e-18 | 72.0 |
| Msa1181680 | MtrunA17_Chr7g0260561 | 45.122 | 82 | 39 | 4 | 1 | 79 | 1 | 79 | 6.05e-18 | 70.9 |
| Msa1181680 | MtrunA17_Chr8g0345161 | 48.052 | 77 | 36 | 2 | 3 | 79 | 4 | 76 | 5.95e-17 | 68.2 |
| Msa1181680 | MtrunA17_Chr8g0357271 | 48.148 | 81 | 37 | 4 | 1 | 79 | 1 | 78 | 2.74e-16 | 66.6 |
| Msa1181680 | MtrunA17_Chr8g0339481 | 42.500 | 80 | 45 | 1 | 1 | 79 | 1 | 80 | 1.07e-13 | 60.1 |
| Msa1181680 | MtrunA17_Chr8g0339571 | 48.101 | 79 | 38 | 2 | 1 | 79 | 9 | 84 | 1.03e-12 | 57.8 |
| Msa1181680 | MtrunA17_Chr8g0339501 | 43.750 | 64 | 35 | 1 | 1 | 63 | 1 | 64 | 2.03e-12 | 57.8 |
| Msa1181680 | MtrunA17_Chr8g0359831 | 40.260 | 77 | 41 | 2 | 4 | 79 | 5 | 77 | 3.26e-12 | 56.2 |
| Msa1181680 | MtrunA17_Chr4g0064831 | 43.038 | 79 | 40 | 4 | 1 | 76 | 1 | 77 | 1.73e-11 | 54.3 |
| Msa1181680 | MtrunA17_Chr8g0339731 | 57.143 | 42 | 18 | 0 | 1 | 42 | 1 | 42 | 5.19e-11 | 52.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1181680 | AT1G19610.1 | 51.351 | 74 | 33 | 1 | 9 | 79 | 5 | 78 | 7.24e-22 | 80.9 |
| Msa1181680 | AT2G26020.1 | 39.744 | 78 | 42 | 1 | 7 | 79 | 3 | 80 | 9.05e-13 | 58.2 |
| Msa1181680 | AT5G44420.1 | 37.179 | 78 | 44 | 1 | 7 | 79 | 3 | 80 | 1.46e-11 | 55.1 |
| Msa1181680 | AT5G44430.1 | 42.308 | 78 | 40 | 2 | 7 | 79 | 3 | 80 | 1.77e-11 | 54.7 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCCTTATTTCCACATC+TGG | 0.345226 | 8_1:-16122619 | Msa1181680:intron |
| TTGTACCAGATGTGGAAATA+AGG | 0.362903 | 8_1:+16122614 | None:intergenic |
| GCTTGTCATCGTCAAGGCTT+TGG | 0.378692 | 8_1:-16122396 | Msa1181680:CDS |
| GATGTGGAGCAAGCTACTTT+TGG | 0.461271 | 8_1:-16122420 | Msa1181680:CDS |
| AAACGAAGTAAAACTTGGTC+AGG | 0.485517 | 8_1:-16122483 | Msa1181680:CDS |
| TGATTAGTTTGTACCAGATG+TGG | 0.519499 | 8_1:+16122606 | None:intergenic |
| GGTGTAAAATTTATTAGCAG+AGG | 0.526587 | 8_1:+16122666 | None:intergenic |
| CAGTTTCCTGTGTTAAGACA+AGG | 0.529102 | 8_1:+16122459 | None:intergenic |
| TTTGGTGCTTGTCATCGTCA+AGG | 0.545774 | 8_1:-16122402 | Msa1181680:CDS |
| CAGATGTGGAAATAAGGAGA+AGG | 0.597896 | 8_1:+16122620 | None:intergenic |
| AGATGTGGAAATAAGGAGAA+GGG | 0.609701 | 8_1:+16122621 | None:intergenic |
| TAAAAGACAATGCGTTGATG+TGG | 0.623871 | 8_1:-16122436 | Msa1181680:CDS |
| TGGAAATAAGGAGAAGGGCA+AGG | 0.634862 | 8_1:+16122626 | None:intergenic |
| TCAGGACCTTGTCTTAACAC+AGG | 0.672794 | 8_1:-16122465 | Msa1181680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAACAGTTATTAATAT+AGG | + | chr8_1:16122463-16122482 | None:intergenic | 15.0% |
| !!! | TTTATTTTTTTTATGTGTAG+AGG | - | chr8_1:16122512-16122531 | Msa1181680:intron | 15.0% |
| !! | ACAAGTTAAGAACATAAATA+AGG | + | chr8_1:16122492-16122511 | None:intergenic | 20.0% |
| !!! | ATTTTTTTTATGTGTAGAGG+TGG | - | chr8_1:16122515-16122534 | Msa1181680:intron | 25.0% |
| GGTGTAAAATTTATTAGCAG+AGG | + | chr8_1:16122375-16122394 | None:intergenic | 30.0% | |
| GTCAAAAACGAAGTAAAACT+TGG | - | chr8_1:16122550-16122569 | Msa1181680:intron | 30.0% | |
| AAGGCAGACAAAAACAAAGA+TGG | + | chr8_1:16122396-16122415 | None:intergenic | 35.0% | |
| AGATGTGGAAATAAGGAGAA+GGG | + | chr8_1:16122420-16122439 | None:intergenic | 35.0% | |
| TTGTACCAGATGTGGAAATA+AGG | + | chr8_1:16122427-16122446 | None:intergenic | 35.0% | |
| !! | TGATTAGTTTGTACCAGATG+TGG | + | chr8_1:16122435-16122454 | None:intergenic | 35.0% |
| AAACGAAGTAAAACTTGGTC+AGG | - | chr8_1:16122555-16122574 | Msa1181680:intron | 35.0% | |
| ! | TAAAAGACAATGCGTTGATG+TGG | - | chr8_1:16122602-16122621 | Msa1181680:intron | 35.0% |
| CAGATGTGGAAATAAGGAGA+AGG | + | chr8_1:16122421-16122440 | None:intergenic | 40.0% | |
| ! | CTTCTCCTTATTTCCACATC+TGG | - | chr8_1:16122419-16122438 | Msa1181680:CDS | 40.0% |
| CAGTTTCCTGTGTTAAGACA+AGG | + | chr8_1:16122582-16122601 | None:intergenic | 40.0% | |
| TGGAAATAAGGAGAAGGGCA+AGG | + | chr8_1:16122415-16122434 | None:intergenic | 45.0% | |
| TCAGGACCTTGTCTTAACAC+AGG | - | chr8_1:16122573-16122592 | Msa1181680:intron | 45.0% | |
| !! | GATGTGGAGCAAGCTACTTT+TGG | - | chr8_1:16122618-16122637 | Msa1181680:intron | 45.0% |
| !! | TTTGGTGCTTGTCATCGTCA+AGG | - | chr8_1:16122636-16122655 | Msa1181680:CDS | 45.0% |
| GCTTGTCATCGTCAAGGCTT+TGG | - | chr8_1:16122642-16122661 | Msa1181680:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 16122365 | 16122695 | 16122365 | ID=Msa1181680;Name=Msa1181680 |
| chr8_1 | mRNA | 16122365 | 16122695 | 16122365 | ID=Msa1181680-mRNA-1;Parent=Msa1181680;Name=Msa1181680-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|79 |
| chr8_1 | exon | 16122620 | 16122695 | 16122620 | ID=Msa1181680-mRNA-1:exon:6067;Parent=Msa1181680-mRNA-1 |
| chr8_1 | exon | 16122365 | 16122528 | 16122365 | ID=Msa1181680-mRNA-1:exon:6066;Parent=Msa1181680-mRNA-1 |
| chr8_1 | CDS | 16122620 | 16122695 | 16122620 | ID=Msa1181680-mRNA-1:cds;Parent=Msa1181680-mRNA-1 |
| chr8_1 | CDS | 16122365 | 16122528 | 16122365 | ID=Msa1181680-mRNA-1:cds;Parent=Msa1181680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
