Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067125.t1 | AFK42793.1 | 99 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 4.90E-148 | 533.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067125.t1 | Q84X53 | 35.9 | 237 | 151 | 1 | 56 | 291 | 33 | 269 | 2.6e-33 | 144.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067125.t1 | G7IIY0 | 99.0 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 3.5e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067125.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene067125 | 0.828254 | 9.96E-55 | -1.69E-46 |
| MS.gene049356 | MS.gene067125 | 0.832408 | 9.62E-56 | -1.69E-46 |
| MS.gene049369 | MS.gene067125 | 0.838644 | 2.54E-57 | -1.69E-46 |
| MS.gene049427 | MS.gene067125 | 0.834701 | 2.57E-56 | -1.69E-46 |
| MS.gene049491 | MS.gene067125 | 0.815448 | 9.20E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene067125 | 0.806169 | 9.37E-50 | -1.69E-46 |
| MS.gene049614 | MS.gene067125 | 0.821212 | 4.55E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene067125 | 0.851777 | 7.12E-61 | -1.69E-46 |
| MS.gene049662 | MS.gene067125 | 0.843236 | 1.59E-58 | -1.69E-46 |
| MS.gene049805 | MS.gene067125 | 0.800263 | 1.56E-48 | -1.69E-46 |
| MS.gene049828 | MS.gene067125 | 0.80274 | 4.86E-49 | -1.69E-46 |
| MS.gene049907 | MS.gene067125 | 0.817403 | 3.36E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene067125 | 0.870172 | 1.78E-66 | -1.69E-46 |
| MS.gene050301 | MS.gene067125 | 0.845613 | 3.64E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene067125 | 0.830551 | 2.76E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene067125 | 0.909182 | 7.55E-82 | -1.69E-46 |
| MS.gene050520 | MS.gene067125 | 0.819918 | 9.03E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene067125 | 0.803263 | 3.79E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene067125 | 0.814756 | 1.31E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene067125 | 0.876771 | 1.07E-68 | -1.69E-46 |
| MS.gene050770 | MS.gene067125 | 0.876022 | 1.94E-68 | -1.69E-46 |
| MS.gene050772 | MS.gene067125 | 0.819788 | 9.66E-53 | -1.69E-46 |
| MS.gene050783 | MS.gene067125 | 0.812285 | 4.58E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene067125 | 0.847353 | 1.22E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene067125 | 0.848752 | 5.02E-60 | -1.69E-46 |
| MS.gene051228 | MS.gene067125 | 0.827071 | 1.92E-54 | -1.69E-46 |
| MS.gene051255 | MS.gene067125 | 0.823289 | 1.50E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene067125 | 0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene067125 | 0.803085 | 4.13E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene067125 | 0.806219 | 9.15E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene067125 | 0.819405 | 1.18E-52 | -1.69E-46 |
| MS.gene051810 | MS.gene067125 | 0.801142 | 1.04E-48 | -1.69E-46 |
| MS.gene051877 | MS.gene067125 | 0.827012 | 1.98E-54 | -1.69E-46 |
| MS.gene052078 | MS.gene067125 | 0.831425 | 1.68E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene067125 | 0.810002 | 1.43E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene067125 | 0.807128 | 5.88E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene067125 | 0.831826 | 1.34E-55 | -1.69E-46 |
| MS.gene052287 | MS.gene067125 | 0.818013 | 2.45E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene067125 | 0.835838 | 1.33E-56 | -1.69E-46 |
| MS.gene052310 | MS.gene067125 | 0.842891 | 1.96E-58 | -1.69E-46 |
| MS.gene052326 | MS.gene067125 | 0.853509 | 2.28E-61 | -1.69E-46 |
| MS.gene052340 | MS.gene067125 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene067125 | 0.815707 | 8.05E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene067125 | 0.819795 | 9.63E-53 | -1.69E-46 |
| MS.gene052484 | MS.gene067125 | 0.822125 | 2.80E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene067125 | 0.841663 | 4.15E-58 | -1.69E-46 |
| MS.gene052508 | MS.gene067125 | 0.810246 | 1.27E-50 | -1.69E-46 |
| MS.gene052510 | MS.gene067125 | 0.807165 | 5.77E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene067125 | 0.82502 | 5.89E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene067125 | 0.841423 | 4.80E-58 | -1.69E-46 |
| MS.gene053207 | MS.gene067125 | 0.830174 | 3.41E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene067125 | 0.878981 | 1.81E-69 | -1.69E-46 |
| MS.gene053213 | MS.gene067125 | 0.823282 | 1.51E-53 | -1.69E-46 |
| MS.gene053286 | MS.gene067125 | 0.812558 | 3.99E-51 | -1.69E-46 |
| MS.gene053322 | MS.gene067125 | 0.846853 | 1.67E-59 | -1.69E-46 |
| MS.gene053557 | MS.gene067125 | 0.80508 | 1.59E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene067125 | 0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene067125 | 0.839768 | 1.30E-57 | -1.69E-46 |
| MS.gene054123 | MS.gene067125 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene067125 | 0.881683 | 1.96E-70 | -1.69E-46 |
| MS.gene05455 | MS.gene067125 | 0.809701 | 1.66E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene067125 | 0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene054802 | MS.gene067125 | 0.842908 | 1.94E-58 | -1.69E-46 |
| MS.gene054803 | MS.gene067125 | 0.837042 | 6.56E-57 | -1.69E-46 |
| MS.gene054804 | MS.gene067125 | 0.843354 | 1.48E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene067125 | 0.844523 | 7.17E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene067125 | 0.850259 | 1.91E-60 | -1.69E-46 |
| MS.gene055254 | MS.gene067125 | 0.846339 | 2.31E-59 | -1.69E-46 |
| MS.gene055277 | MS.gene067125 | 0.886287 | 3.90E-72 | -1.69E-46 |
| MS.gene055390 | MS.gene067125 | 0.84596 | 2.93E-59 | -1.69E-46 |
| MS.gene055397 | MS.gene067125 | 0.844391 | 7.78E-59 | -1.69E-46 |
| MS.gene055412 | MS.gene067125 | 0.814871 | 1.24E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene067125 | 0.811332 | 7.38E-51 | -1.69E-46 |
| MS.gene055428 | MS.gene067125 | 0.826659 | 2.40E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene067125 | 0.860735 | 1.68E-63 | -1.69E-46 |
| MS.gene055647 | MS.gene067125 | 0.816295 | 5.95E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene067125 | 0.837229 | 5.88E-57 | -1.69E-46 |
| MS.gene05592 | MS.gene067125 | 0.857633 | 1.43E-62 | -1.69E-46 |
| MS.gene056191 | MS.gene067125 | 0.858241 | 9.43E-63 | -1.69E-46 |
| MS.gene056374 | MS.gene067125 | 0.817985 | 2.48E-52 | -1.69E-46 |
| MS.gene056407 | MS.gene067125 | 0.837527 | 4.93E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene067125 | 0.854764 | 9.91E-62 | -1.69E-46 |
| MS.gene056452 | MS.gene067125 | 0.805297 | 1.43E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene067125 | 0.807576 | 4.73E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene067125 | 0.867484 | 1.32E-65 | -1.69E-46 |
| MS.gene056612 | MS.gene067125 | 0.805343 | 1.40E-49 | -1.69E-46 |
| MS.gene056897 | MS.gene067125 | 0.816078 | 6.66E-52 | -1.69E-46 |
| MS.gene056931 | MS.gene067125 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene056942 | MS.gene067125 | 0.800006 | 1.76E-48 | -1.69E-46 |
| MS.gene057011 | MS.gene067125 | 0.829462 | 5.08E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene067125 | 0.821418 | 4.08E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene067125 | 0.81585 | 7.48E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene067125 | 0.852402 | 4.73E-61 | -1.69E-46 |
| MS.gene057146 | MS.gene067125 | 0.826417 | 2.74E-54 | -1.69E-46 |
| MS.gene057295 | MS.gene067125 | 0.802256 | 6.12E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene067125 | 0.836498 | 9.03E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene067125 | 0.802766 | 4.80E-49 | -1.69E-46 |
| MS.gene05735 | MS.gene067125 | 0.818646 | 1.76E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene067125 | 0.815457 | 9.16E-52 | -1.69E-46 |
| MS.gene057448 | MS.gene067125 | 0.801616 | 8.28E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene067125 | 0.807366 | 5.24E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene067125 | 0.802693 | 4.97E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene067125 | 0.800337 | 1.51E-48 | -1.69E-46 |
| MS.gene057926 | MS.gene067125 | 0.814912 | 1.21E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene067125 | 0.813903 | 2.02E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene067125 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene067125 | 0.820583 | 6.35E-53 | -1.69E-46 |
| MS.gene058082 | MS.gene067125 | 0.814842 | 1.25E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene067125 | 0.812164 | 4.87E-51 | -1.69E-46 |
| MS.gene058233 | MS.gene067125 | 0.804049 | 2.60E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene067125 | 0.863488 | 2.40E-64 | -1.69E-46 |
| MS.gene058323 | MS.gene067125 | 0.8425 | 2.49E-58 | -1.69E-46 |
| MS.gene058365 | MS.gene067125 | 0.842331 | 2.76E-58 | -1.69E-46 |
| MS.gene058459 | MS.gene067125 | 0.800459 | 1.43E-48 | -1.69E-46 |
| MS.gene058660 | MS.gene067125 | 0.871437 | 6.84E-67 | -1.69E-46 |
| MS.gene058787 | MS.gene067125 | 0.831864 | 1.31E-55 | -1.69E-46 |
| MS.gene058903 | MS.gene067125 | 0.801908 | 7.21E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene067125 | 0.806379 | 8.46E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene067125 | 0.829683 | 4.49E-55 | -1.69E-46 |
| MS.gene059366 | MS.gene067125 | 0.819256 | 1.28E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene067125 | 0.801327 | 9.49E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene067125 | 0.831188 | 1.92E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene067125 | 0.81195 | 5.42E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene067125 | 0.836551 | 8.76E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene067125 | 0.861427 | 1.03E-63 | -1.69E-46 |
| MS.gene059692 | MS.gene067125 | 0.873619 | 1.28E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene067125 | 0.871912 | 4.76E-67 | -1.69E-46 |
| MS.gene059708 | MS.gene067125 | 0.818995 | 1.46E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene067125 | 0.829262 | 5.68E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene067125 | 0.821164 | 4.67E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene067125 | 0.843535 | 1.32E-58 | -1.69E-46 |
| MS.gene059974 | MS.gene067125 | 0.84414 | 9.09E-59 | -1.69E-46 |
| MS.gene060005 | MS.gene067125 | 0.831151 | 1.96E-55 | -1.69E-46 |
| MS.gene060356 | MS.gene067125 | 0.821686 | 3.54E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene067125 | 0.879945 | 8.23E-70 | -1.69E-46 |
| MS.gene060569 | MS.gene067125 | 0.810902 | 9.15E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene067125 | 0.88013 | 7.07E-70 | -1.69E-46 |
| MS.gene060818 | MS.gene067125 | 0.826497 | 2.63E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene067125 | 0.818391 | 2.01E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene067125 | 0.840071 | 1.08E-57 | -1.69E-46 |
| MS.gene061041 | MS.gene067125 | 0.863878 | 1.82E-64 | -1.69E-46 |
| MS.gene061049 | MS.gene067125 | 0.843684 | 1.20E-58 | -1.69E-46 |
| MS.gene061051 | MS.gene067125 | -0.860605 | 1.84E-63 | -1.69E-46 |
| MS.gene061200 | MS.gene067125 | 0.856638 | 2.81E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067125 | MS.gene92189 | PPI |
| MS.gene067125 | MS.gene77650 | PPI |
| MS.gene067125 | MS.gene039043 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067125.t1 | MTR_2g049780 | 99.000 | 300 | 3 | 0 | 1 | 300 | 14 | 313 | 0.0 | 603 |
| MS.gene067125.t1 | MTR_5g041630 | 42.491 | 273 | 151 | 1 | 30 | 296 | 25 | 297 | 3.68e-68 | 214 |
| MS.gene067125.t1 | MTR_2g067230 | 39.076 | 238 | 140 | 2 | 56 | 293 | 69 | 301 | 4.93e-57 | 187 |
| MS.gene067125.t1 | MTR_7g081270 | 40.654 | 214 | 123 | 4 | 81 | 291 | 67 | 279 | 2.27e-42 | 147 |
| MS.gene067125.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 322 | 549 | 2.18e-25 | 106 |
| MS.gene067125.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 261 | 488 | 2.20e-25 | 105 |
| MS.gene067125.t1 | MTR_2g034600 | 30.165 | 242 | 154 | 6 | 57 | 297 | 326 | 553 | 2.27e-25 | 105 |
| MS.gene067125.t1 | MTR_4g070060 | 28.112 | 249 | 170 | 6 | 55 | 296 | 344 | 590 | 1.80e-21 | 94.7 |
| MS.gene067125.t1 | MTR_5g068860 | 26.136 | 264 | 154 | 7 | 69 | 293 | 223 | 484 | 1.75e-19 | 89.0 |
| MS.gene067125.t1 | MTR_6g003960 | 25.000 | 228 | 159 | 3 | 70 | 291 | 366 | 587 | 5.80e-18 | 84.3 |
| MS.gene067125.t1 | MTR_5g094610 | 24.215 | 223 | 154 | 4 | 57 | 268 | 227 | 445 | 1.66e-17 | 82.8 |
| MS.gene067125.t1 | MTR_4g084630 | 23.600 | 250 | 181 | 4 | 52 | 291 | 69 | 318 | 3.86e-17 | 80.9 |
| MS.gene067125.t1 | MTR_1g115415 | 25.556 | 270 | 160 | 5 | 63 | 293 | 185 | 452 | 4.93e-17 | 81.6 |
| MS.gene067125.t1 | MTR_0320s0040 | 35.556 | 90 | 57 | 1 | 56 | 145 | 48 | 136 | 2.92e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067125.t1 | AT2G34620 | 66.212 | 293 | 92 | 3 | 9 | 295 | 3 | 294 | 1.84e-129 | 370 |
| MS.gene067125.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 32 | 268 | 7.64e-70 | 218 |
| MS.gene067125.t1 | AT2G03050 | 48.101 | 237 | 122 | 1 | 56 | 291 | 33 | 269 | 9.57e-70 | 218 |
| MS.gene067125.t1 | AT2G36000 | 37.037 | 243 | 144 | 4 | 58 | 296 | 75 | 312 | 4.31e-51 | 171 |
| MS.gene067125.t1 | AT2G36000 | 37.037 | 243 | 144 | 4 | 58 | 296 | 75 | 312 | 5.56e-51 | 171 |
| MS.gene067125.t1 | AT3G18870 | 37.705 | 244 | 143 | 4 | 51 | 291 | 31 | 268 | 5.91e-46 | 156 |
| MS.gene067125.t1 | AT2G21710 | 28.340 | 247 | 168 | 6 | 57 | 296 | 353 | 597 | 3.38e-24 | 102 |
| MS.gene067125.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 1.07e-21 | 95.1 |
| MS.gene067125.t1 | AT4G14605 | 29.787 | 235 | 160 | 5 | 58 | 291 | 237 | 467 | 1.07e-21 | 95.1 |
| MS.gene067125.t1 | AT1G78930 | 25.328 | 229 | 167 | 2 | 65 | 291 | 348 | 574 | 1.59e-19 | 89.0 |
| MS.gene067125.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 226 | 443 | 7.02e-17 | 81.3 |
| MS.gene067125.t1 | AT5G55580 | 24.771 | 218 | 160 | 4 | 60 | 273 | 120 | 337 | 7.55e-17 | 80.5 |
| MS.gene067125.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 4.46e-16 | 79.0 |
| MS.gene067125.t1 | AT4G02990 | 26.667 | 270 | 157 | 8 | 63 | 293 | 225 | 492 | 4.46e-16 | 79.0 |
| MS.gene067125.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.17e-15 | 76.6 |
| MS.gene067125.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.17e-15 | 76.6 |
| MS.gene067125.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.60e-15 | 76.6 |
| MS.gene067125.t1 | AT4G38160 | 24.506 | 253 | 180 | 4 | 52 | 293 | 70 | 322 | 1.79e-15 | 76.3 |
| MS.gene067125.t1 | AT2G44020 | 25.481 | 208 | 119 | 2 | 86 | 293 | 286 | 457 | 3.95e-15 | 75.9 |
| MS.gene067125.t1 | AT2G21710 | 25.243 | 206 | 145 | 6 | 57 | 255 | 353 | 556 | 3.29e-13 | 70.1 |
| MS.gene067125.t1 | AT2G21710 | 24.878 | 205 | 145 | 6 | 57 | 254 | 353 | 555 | 2.52e-12 | 67.4 |
Find 58 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTAAGCATTTGGAGAGTA+TGG | 0.202455 | 2.4:+41836159 | MS.gene067125:CDS |
| AAAGTACTGATGATGAATTT+AGG | 0.234140 | 2.4:+41836419 | MS.gene067125:CDS |
| TTATCAAGATTGTGTTCTTT+TGG | 0.240932 | 2.4:+41836105 | MS.gene067125:CDS |
| ATACTTGAAGTAAGAATCTT+AGG | 0.245965 | 2.4:-41835902 | None:intergenic |
| TTTGGGTTTGGGAGTTAAAT+TGG | 0.262027 | 2.4:-41835651 | None:intergenic |
| GAAGTAATGGGCATTGATTC+AGG | 0.276282 | 2.4:+41835755 | MS.gene067125:CDS |
| TGTGGAAATGAAGGGGAAAT+TGG | 0.285794 | 2.4:+41836282 | MS.gene067125:CDS |
| AAGTACTGATGATGAATTTA+GGG | 0.305836 | 2.4:+41836420 | MS.gene067125:CDS |
| GGACACATGCCAAAGATTCT+TGG | 0.310114 | 2.4:-41835881 | None:intergenic |
| AAAGACCTTCCAAGAATCTT+TGG | 0.314251 | 2.4:+41835872 | MS.gene067125:CDS |
| AAGGTAGCTGTATGAAGGTT+AGG | 0.324878 | 2.4:-41835797 | None:intergenic |
| GTCTTTATGTTGGATACCTT+TGG | 0.333066 | 2.4:-41835855 | None:intergenic |
| AATGGAATGAATGGATTCTA+AGG | 0.339360 | 2.4:-41835816 | None:intergenic |
| TATTCCTAAACTTAAGCATT+TGG | 0.349186 | 2.4:+41836150 | MS.gene067125:CDS |
| CTCTCCAAATGCTTAAGTTT+AGG | 0.351147 | 2.4:-41836154 | None:intergenic |
| ATTCTTTGTCTTGAAGTAAT+GGG | 0.356794 | 2.4:+41835743 | MS.gene067125:CDS |
| TAGTTTGGAGAATAGGATAA+AGG | 0.362241 | 2.4:+41836339 | MS.gene067125:CDS |
| GGAGACAAGAAAGGTTATAA+TGG | 0.387380 | 2.4:-41835834 | None:intergenic |
| GATTCTTTGTCTTGAAGTAA+TGG | 0.416623 | 2.4:+41835742 | MS.gene067125:CDS |
| ACACTAGAAGTAAGCAATCT+TGG | 0.430968 | 2.4:-41836013 | None:intergenic |
| AAAGGTTATAATGGAATGAA+TGG | 0.434056 | 2.4:-41835825 | None:intergenic |
| ATTAAGAGATTTAGAAGCAT+TGG | 0.444652 | 2.4:+41836081 | MS.gene067125:CDS |
| TTTAGGGAGTTGATAAAGAA+GGG | 0.454349 | 2.4:+41836436 | MS.gene067125:CDS |
| AGAGCTATGGTTCTAGCAGC+TGG | 0.458017 | 2.4:-41835572 | None:intergenic |
| GGTTAGACACATGGAGGTTG+TGG | 0.463307 | 2.4:+41836360 | MS.gene067125:CDS |
| TTCTTGGAAGGTCTTTATGT+TGG | 0.468294 | 2.4:-41835865 | None:intergenic |
| CACATGGAGGTTGTGGAAAG+TGG | 0.473398 | 2.4:+41836367 | MS.gene067125:CDS |
| TTGGTTGAAGGAGGTGATGA+TGG | 0.480821 | 2.4:-41835632 | None:intergenic |
| AGGTAGCTGTATGAAGGTTA+GGG | 0.481780 | 2.4:-41835796 | None:intergenic |
| TAAAGAAGAGGCTAGGTGTA+TGG | 0.485234 | 2.4:+41836189 | MS.gene067125:CDS |
| CTTAAGCATTTGGAGAGTAT+GGG | 0.487893 | 2.4:+41836160 | MS.gene067125:CDS |
| GATACCTTTGGAGACAAGAA+AGG | 0.497187 | 2.4:-41835843 | None:intergenic |
| ATTTAGGGAGTTGATAAAGA+AGG | 0.499226 | 2.4:+41836435 | MS.gene067125:CDS |
| TTGGGAGTTAAATTGGTTGA+AGG | 0.501320 | 2.4:-41835644 | None:intergenic |
| GGAGTTAAATTGGTTGAAGG+AGG | 0.504389 | 2.4:-41835641 | None:intergenic |
| TCATGAGTTGGTGTGTAAAG+TGG | 0.520744 | 2.4:-41835692 | None:intergenic |
| ATAACCTTTCTTGTCTCCAA+AGG | 0.526401 | 2.4:+41835839 | MS.gene067125:CDS |
| CTGTTAAGATAAAACAAAGC+TGG | 0.538212 | 2.4:-41836052 | None:intergenic |
| ATCAGATGAGACTGAACATA+GGG | 0.547332 | 2.4:-41835606 | None:intergenic |
| AGGCTAGGTGTATGGTGTTG+AGG | 0.553273 | 2.4:+41836197 | MS.gene067125:CDS |
| ATTGCTTACTTCTAGTGTGG+TGG | 0.553869 | 2.4:+41836018 | MS.gene067125:CDS |
| ATACTAAAAGTCAACAATGC+AGG | 0.557976 | 2.4:-41836223 | None:intergenic |
| AAGATTGCTTACTTCTAGTG+TGG | 0.564951 | 2.4:+41836015 | MS.gene067125:CDS |
| GGAAGATGAGTGTAGAGCTA+TGG | 0.568612 | 2.4:-41835585 | None:intergenic |
| ATTCTAAGGTAGCTGTATGA+AGG | 0.573875 | 2.4:-41835802 | None:intergenic |
| TAGGGAGTTGATAAAGAAGG+GGG | 0.580226 | 2.4:+41836438 | MS.gene067125:CDS |
| GGAAATGAAGGGGAAATTGG+AGG | 0.580999 | 2.4:+41836285 | MS.gene067125:CDS |
| ACATGGAGGTTGTGGAAAGT+GGG | 0.587398 | 2.4:+41836368 | MS.gene067125:CDS |
| GAGCTATGGTTCTAGCAGCT+GGG | 0.599605 | 2.4:-41835571 | None:intergenic |
| TATGGGATTTACTAAAGAAG+AGG | 0.599972 | 2.4:+41836177 | MS.gene067125:CDS |
| TATCAGATGAGACTGAACAT+AGG | 0.607408 | 2.4:-41835607 | None:intergenic |
| TTAGGGAGTTGATAAAGAAG+GGG | 0.610325 | 2.4:+41836437 | MS.gene067125:CDS |
| GAGTTGGTGTGTAAAGTGGA+TGG | 0.622825 | 2.4:-41835688 | None:intergenic |
| GATTTACTAAAGAAGAGGCT+AGG | 0.635507 | 2.4:+41836182 | MS.gene067125:CDS |
| TAGGATAAAGGTTAGACACA+TGG | 0.648833 | 2.4:+41836351 | MS.gene067125:CDS |
| ACATGCCAAAGATTCTTGGA+AGG | 0.652767 | 2.4:-41835877 | None:intergenic |
| AAACTAAAAGAGAAATACTG+AGG | 0.668925 | 2.4:-41836322 | None:intergenic |
| GATAAAGGTTAGACACATGG+AGG | 0.698797 | 2.4:+41836354 | MS.gene067125:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAGAAAAATACTCAAATTT+AGG | - | chr2.4:41836265-41836284 | None:intergenic | 20.0% |
| !!! | TAAATTTGAGTATTTTTCTG+TGG | + | chr2.4:41836264-41836283 | MS.gene067125:CDS | 20.0% |
| !!! | TTGTTTTATCTTAACAGATT+AGG | + | chr2.4:41836058-41836077 | MS.gene067125:CDS | 20.0% |
| ! | AAACTAAAAGAGAAATACTG+AGG | - | chr2.4:41836325-41836344 | None:intergenic | 25.0% |
| ! | ATACTTGAAGTAAGAATCTT+AGG | - | chr2.4:41835905-41835924 | None:intergenic | 25.0% |
| ! | ATTCTTTGTCTTGAAGTAAT+GGG | + | chr2.4:41835743-41835762 | MS.gene067125:CDS | 25.0% |
| ! | TATTCCTAAACTTAAGCATT+TGG | + | chr2.4:41836150-41836169 | MS.gene067125:CDS | 25.0% |
| ! | TGTATGAGAAAATCAAAAAC+TGG | - | chr2.4:41835944-41835963 | None:intergenic | 25.0% |
| !! | AAAGGTTATAATGGAATGAA+TGG | - | chr2.4:41835828-41835847 | None:intergenic | 25.0% |
| !! | ATTAAGAGATTTAGAAGCAT+TGG | + | chr2.4:41836081-41836100 | MS.gene067125:CDS | 25.0% |
| !! | TATTTTTCTGTGGAAATGAA+GGG | + | chr2.4:41836274-41836293 | MS.gene067125:CDS | 25.0% |
| !! | TTATCAAGATTGTGTTCTTT+TGG | + | chr2.4:41836105-41836124 | MS.gene067125:CDS | 25.0% |
| !!! | AAAGTACTGATGATGAATTT+AGG | + | chr2.4:41836419-41836438 | MS.gene067125:CDS | 25.0% |
| !!! | AAGTACTGATGATGAATTTA+GGG | + | chr2.4:41836420-41836439 | MS.gene067125:CDS | 25.0% |
| !!! | TCAGTATTTCTCTTTTAGTT+TGG | + | chr2.4:41836324-41836343 | MS.gene067125:CDS | 25.0% |
| ATACTAAAAGTCAACAATGC+AGG | - | chr2.4:41836226-41836245 | None:intergenic | 30.0% | |
| CTGTTAAGATAAAACAAAGC+TGG | - | chr2.4:41836055-41836074 | None:intergenic | 30.0% | |
| GATTCTTTGTCTTGAAGTAA+TGG | + | chr2.4:41835742-41835761 | MS.gene067125:CDS | 30.0% | |
| TAGTTTGGAGAATAGGATAA+AGG | + | chr2.4:41836339-41836358 | MS.gene067125:CDS | 30.0% | |
| TATGGGATTTACTAAAGAAG+AGG | + | chr2.4:41836177-41836196 | MS.gene067125:CDS | 30.0% | |
| ! | AATGGAATGAATGGATTCTA+AGG | - | chr2.4:41835819-41835838 | None:intergenic | 30.0% |
| ! | ATTTTTCTGTGGAAATGAAG+GGG | + | chr2.4:41836275-41836294 | MS.gene067125:CDS | 30.0% |
| ! | CTTTTAAGCATCAATGACAA+AGG | - | chr2.4:41836403-41836422 | None:intergenic | 30.0% |
| ! | GTATTTTTCTGTGGAAATGA+AGG | + | chr2.4:41836273-41836292 | MS.gene067125:CDS | 30.0% |
| !! | ATTTAGGGAGTTGATAAAGA+AGG | + | chr2.4:41836435-41836454 | MS.gene067125:CDS | 30.0% |
| !! | TTTAGGGAGTTGATAAAGAA+GGG | + | chr2.4:41836436-41836455 | MS.gene067125:CDS | 30.0% |
| !!! | ACTTTTCTAAAGCTATGATC+AGG | - | chr2.4:41835980-41835999 | None:intergenic | 30.0% |
| !!! | TCTCTTTTAGTTTGGAGAAT+AGG | + | chr2.4:41836332-41836351 | MS.gene067125:CDS | 30.0% |
| !!! | TTTTGGTTTCTAACGTTGAA+AGG | + | chr2.4:41836122-41836141 | MS.gene067125:CDS | 30.0% |
| AAAGACCTTCCAAGAATCTT+TGG | + | chr2.4:41835872-41835891 | MS.gene067125:CDS | 35.0% | |
| AAGATTGCTTACTTCTAGTG+TGG | + | chr2.4:41836015-41836034 | MS.gene067125:CDS | 35.0% | |
| ACACTAGAAGTAAGCAATCT+TGG | - | chr2.4:41836016-41836035 | None:intergenic | 35.0% | |
| ACTTAAGCATTTGGAGAGTA+TGG | + | chr2.4:41836159-41836178 | MS.gene067125:CDS | 35.0% | |
| ATAACCTTTCTTGTCTCCAA+AGG | + | chr2.4:41835839-41835858 | MS.gene067125:CDS | 35.0% | |
| ATCAGATGAGACTGAACATA+GGG | - | chr2.4:41835609-41835628 | None:intergenic | 35.0% | |
| CTCTCCAAATGCTTAAGTTT+AGG | - | chr2.4:41836157-41836176 | None:intergenic | 35.0% | |
| GATTTACTAAAGAAGAGGCT+AGG | + | chr2.4:41836182-41836201 | MS.gene067125:CDS | 35.0% | |
| GGAGACAAGAAAGGTTATAA+TGG | - | chr2.4:41835837-41835856 | None:intergenic | 35.0% | |
| GTCTTTATGTTGGATACCTT+TGG | - | chr2.4:41835858-41835877 | None:intergenic | 35.0% | |
| TAGGATAAAGGTTAGACACA+TGG | + | chr2.4:41836351-41836370 | MS.gene067125:CDS | 35.0% | |
| TATCAGATGAGACTGAACAT+AGG | - | chr2.4:41835610-41835629 | None:intergenic | 35.0% | |
| TTCTTGGAAGGTCTTTATGT+TGG | - | chr2.4:41835868-41835887 | None:intergenic | 35.0% | |
| TTGGGAGTTAAATTGGTTGA+AGG | - | chr2.4:41835647-41835666 | None:intergenic | 35.0% | |
| ! | AGTGAGAGTTTTTCATGAGT+TGG | - | chr2.4:41835707-41835726 | None:intergenic | 35.0% |
| ! | CTTAAGCATTTGGAGAGTAT+GGG | + | chr2.4:41836160-41836179 | MS.gene067125:CDS | 35.0% |
| ! | TTTGGGTTTGGGAGTTAAAT+TGG | - | chr2.4:41835654-41835673 | None:intergenic | 35.0% |
| !! | ATTCTAAGGTAGCTGTATGA+AGG | - | chr2.4:41835805-41835824 | None:intergenic | 35.0% |
| !! | TTAGGGAGTTGATAAAGAAG+GGG | + | chr2.4:41836437-41836456 | MS.gene067125:CDS | 35.0% |
| !!! | ATGGTTTTGAAGGAGACTTT+TGG | - | chr2.4:41835672-41835691 | None:intergenic | 35.0% |
| !!! | TGGTTTTGAAGGAGACTTTT+GGG | - | chr2.4:41835671-41835690 | None:intergenic | 35.0% |
| !!! | TGTAAAGTGGATGGTTTTGA+AGG | - | chr2.4:41835682-41835701 | None:intergenic | 35.0% |
| AAGGTAGCTGTATGAAGGTT+AGG | - | chr2.4:41835800-41835819 | None:intergenic | 40.0% | |
| ACATGCCAAAGATTCTTGGA+AGG | - | chr2.4:41835880-41835899 | None:intergenic | 40.0% | |
| AGGTAGCTGTATGAAGGTTA+GGG | - | chr2.4:41835799-41835818 | None:intergenic | 40.0% | |
| ATTGCTTACTTCTAGTGTGG+TGG | + | chr2.4:41836018-41836037 | MS.gene067125:CDS | 40.0% | |
| GATAAAGGTTAGACACATGG+AGG | + | chr2.4:41836354-41836373 | MS.gene067125:CDS | 40.0% | |
| GGAGTTAAATTGGTTGAAGG+AGG | - | chr2.4:41835644-41835663 | None:intergenic | 40.0% | |
| TAAAGAAGAGGCTAGGTGTA+TGG | + | chr2.4:41836189-41836208 | MS.gene067125:CDS | 40.0% | |
| TGTGGAAATGAAGGGGAAAT+TGG | + | chr2.4:41836282-41836301 | MS.gene067125:CDS | 40.0% | |
| ! | GATACCTTTGGAGACAAGAA+AGG | - | chr2.4:41835846-41835865 | None:intergenic | 40.0% |
| !! | GAAGTAATGGGCATTGATTC+AGG | + | chr2.4:41835755-41835774 | MS.gene067125:CDS | 40.0% |
| !! | TAGGGAGTTGATAAAGAAGG+GGG | + | chr2.4:41836438-41836457 | MS.gene067125:CDS | 40.0% |
| !! | TCATGAGTTGGTGTGTAAAG+TGG | - | chr2.4:41835695-41835714 | None:intergenic | 40.0% |
| !!! | TGAAGGAGACTTTTGGGTTT+GGG | - | chr2.4:41835665-41835684 | None:intergenic | 40.0% |
| !!! | TTGAAGGAGACTTTTGGGTT+TGG | - | chr2.4:41835666-41835685 | None:intergenic | 40.0% |
| ACATGGAGGTTGTGGAAAGT+GGG | + | chr2.4:41836368-41836387 | MS.gene067125:CDS | 45.0% | |
| GGAAATGAAGGGGAAATTGG+AGG | + | chr2.4:41836285-41836304 | MS.gene067125:CDS | 45.0% | |
| GGAAGATGAGTGTAGAGCTA+TGG | - | chr2.4:41835588-41835607 | None:intergenic | 45.0% | |
| GGACACATGCCAAAGATTCT+TGG | - | chr2.4:41835884-41835903 | None:intergenic | 45.0% | |
| TTGGTTGAAGGAGGTGATGA+TGG | - | chr2.4:41835635-41835654 | None:intergenic | 45.0% | |
| !! | GAGTTGGTGTGTAAAGTGGA+TGG | - | chr2.4:41835691-41835710 | None:intergenic | 45.0% |
| CACATGGAGGTTGTGGAAAG+TGG | + | chr2.4:41836367-41836386 | MS.gene067125:CDS | 50.0% | |
| GGTTAGACACATGGAGGTTG+TGG | + | chr2.4:41836360-41836379 | MS.gene067125:CDS | 50.0% | |
| ! | AGGCTAGGTGTATGGTGTTG+AGG | + | chr2.4:41836197-41836216 | MS.gene067125:CDS | 50.0% |
| !! | AGAGCTATGGTTCTAGCAGC+TGG | - | chr2.4:41835575-41835594 | None:intergenic | 50.0% |
| !! | GAGCTATGGTTCTAGCAGCT+GGG | - | chr2.4:41835574-41835593 | None:intergenic | 50.0% |
| !! | GAGTTGATAAAGAAGGGGGC+TGG | + | chr2.4:41836442-41836461 | MS.gene067125:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 41835566 | 41836468 | 41835566 | ID=MS.gene067125 |
| chr2.4 | mRNA | 41835566 | 41836468 | 41835566 | ID=MS.gene067125.t1;Parent=MS.gene067125 |
| chr2.4 | exon | 41835566 | 41836468 | 41835566 | ID=MS.gene067125.t1.exon1;Parent=MS.gene067125.t1 |
| chr2.4 | CDS | 41835566 | 41836468 | 41835566 | ID=cds.MS.gene067125.t1;Parent=MS.gene067125.t1 |
| Gene Sequence |
| Protein sequence |
