Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22670.t1 | AFK42539.1 | 99.5 | 213 | 1 | 0 | 1 | 213 | 68 | 280 | 8.90E-112 | 412.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22670.t1 | Q9S726 | 78.4 | 213 | 46 | 0 | 1 | 213 | 64 | 276 | 2.6e-91 | 336.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22670.t1 | G7L1U4 | 99.5 | 213 | 1 | 0 | 1 | 213 | 68 | 280 | 6.4e-112 | 412.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene22670 | 0.86239 | 5.24E-64 | -1.69E-46 |
| MS.gene049173 | MS.gene22670 | 0.841926 | 3.53E-58 | -1.69E-46 |
| MS.gene049181 | MS.gene22670 | -0.81044 | 1.15E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene22670 | 0.851044 | 1.15E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene22670 | 0.880132 | 7.06E-70 | -1.69E-46 |
| MS.gene049232 | MS.gene22670 | 0.86863 | 5.66E-66 | -1.69E-46 |
| MS.gene049285 | MS.gene22670 | 0.908533 | 1.54E-81 | -1.69E-46 |
| MS.gene049320 | MS.gene22670 | 0.90956 | 4.98E-82 | -1.69E-46 |
| MS.gene049609 | MS.gene22670 | 0.802956 | 4.39E-49 | -1.69E-46 |
| MS.gene049627 | MS.gene22670 | 0.813522 | 2.45E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene22670 | 0.860906 | 1.49E-63 | -1.69E-46 |
| MS.gene049865 | MS.gene22670 | 0.803398 | 3.55E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene22670 | 0.870936 | 1.00E-66 | -1.69E-46 |
| MS.gene050163 | MS.gene22670 | 0.90483 | 8.14E-80 | -1.69E-46 |
| MS.gene050165 | MS.gene22670 | 0.923095 | 4.17E-89 | -1.69E-46 |
| MS.gene050170 | MS.gene22670 | 0.817718 | 2.85E-52 | -1.69E-46 |
| MS.gene050216 | MS.gene22670 | 0.818029 | 2.43E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene22670 | 0.85387 | 1.80E-61 | -1.69E-46 |
| MS.gene050333 | MS.gene22670 | 0.893975 | 3.81E-75 | -1.69E-46 |
| MS.gene050444 | MS.gene22670 | 0.826452 | 2.69E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene22670 | 0.855762 | 5.08E-62 | -1.69E-46 |
| MS.gene050504 | MS.gene22670 | 0.896779 | 2.66E-76 | -1.69E-46 |
| MS.gene050518 | MS.gene22670 | 0.941926 | 1.77E-101 | -1.69E-46 |
| MS.gene050577 | MS.gene22670 | 0.848827 | 4.78E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene22670 | 0.917048 | 8.52E-86 | -1.69E-46 |
| MS.gene050662 | MS.gene22670 | 0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene22670 | 0.886351 | 3.69E-72 | -1.69E-46 |
| MS.gene050699 | MS.gene22670 | 0.88529 | 9.24E-72 | -1.69E-46 |
| MS.gene050772 | MS.gene22670 | 0.859456 | 4.08E-63 | -1.69E-46 |
| MS.gene05080 | MS.gene22670 | 0.832216 | 1.07E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene22670 | 0.845967 | 2.92E-59 | -1.69E-46 |
| MS.gene050871 | MS.gene22670 | 0.841514 | 4.54E-58 | -1.69E-46 |
| MS.gene050876 | MS.gene22670 | 0.89767 | 1.12E-76 | -1.69E-46 |
| MS.gene05087 | MS.gene22670 | 0.845062 | 5.13E-59 | -1.69E-46 |
| MS.gene050904 | MS.gene22670 | 0.871498 | 6.53E-67 | -1.69E-46 |
| MS.gene050986 | MS.gene22670 | 0.897925 | 8.76E-77 | -1.69E-46 |
| MS.gene05100 | MS.gene22670 | 0.846351 | 2.29E-59 | -1.69E-46 |
| MS.gene051228 | MS.gene22670 | 0.815213 | 1.04E-51 | -1.69E-46 |
| MS.gene051268 | MS.gene22670 | 0.826503 | 2.62E-54 | -1.69E-46 |
| MS.gene051317 | MS.gene22670 | 0.871498 | 6.53E-67 | -1.69E-46 |
| MS.gene051366 | MS.gene22670 | -0.81147 | 6.89E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene22670 | 0.851471 | 8.69E-61 | -1.69E-46 |
| MS.gene051810 | MS.gene22670 | 0.88448 | 1.85E-71 | -1.69E-46 |
| MS.gene051858 | MS.gene22670 | 0.812507 | 4.09E-51 | -1.69E-46 |
| MS.gene051949 | MS.gene22670 | 0.884001 | 2.78E-71 | -1.69E-46 |
| MS.gene052164 | MS.gene22670 | 0.809107 | 2.23E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene22670 | 0.828283 | 9.80E-55 | -1.69E-46 |
| MS.gene052482 | MS.gene22670 | 0.841491 | 4.60E-58 | -1.69E-46 |
| MS.gene052486 | MS.gene22670 | 0.844132 | 9.14E-59 | -1.69E-46 |
| MS.gene052506 | MS.gene22670 | 0.80237 | 5.80E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene22670 | 0.83498 | 2.19E-56 | -1.69E-46 |
| MS.gene052872 | MS.gene22670 | 0.804915 | 1.72E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene22670 | 0.837229 | 5.88E-57 | -1.69E-46 |
| MS.gene052966 | MS.gene22670 | 0.830939 | 2.21E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene22670 | 0.806381 | 8.46E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene22670 | 0.827919 | 1.20E-54 | -1.69E-46 |
| MS.gene053213 | MS.gene22670 | 0.857846 | 1.24E-62 | -1.69E-46 |
| MS.gene053286 | MS.gene22670 | 0.861273 | 1.15E-63 | -1.69E-46 |
| MS.gene053574 | MS.gene22670 | 0.824904 | 6.27E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene22670 | 0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene053633 | MS.gene22670 | 0.824048 | 9.97E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene22670 | 0.814332 | 1.63E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene22670 | 0.817947 | 2.53E-52 | -1.69E-46 |
| MS.gene054374 | MS.gene22670 | 0.801969 | 7.01E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene22670 | 0.810498 | 1.12E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene22670 | 0.850955 | 1.22E-60 | -1.69E-46 |
| MS.gene054638 | MS.gene22670 | 0.829341 | 5.43E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene22670 | 0.897324 | 1.57E-76 | -1.69E-46 |
| MS.gene05470 | MS.gene22670 | 0.838187 | 3.34E-57 | -1.69E-46 |
| MS.gene054954 | MS.gene22670 | 0.920046 | 2.10E-87 | -1.69E-46 |
| MS.gene055134 | MS.gene22670 | -0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene055204 | MS.gene22670 | -0.804488 | 2.11E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene22670 | 0.888522 | 5.48E-73 | -1.69E-46 |
| MS.gene055502 | MS.gene22670 | 0.872804 | 2.40E-67 | -1.69E-46 |
| MS.gene055577 | MS.gene22670 | 0.85932 | 4.49E-63 | -1.69E-46 |
| MS.gene055634 | MS.gene22670 | 0.879558 | 1.13E-69 | -1.69E-46 |
| MS.gene055636 | MS.gene22670 | 0.820191 | 7.81E-53 | -1.69E-46 |
| MS.gene055638 | MS.gene22670 | 0.843864 | 1.08E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene22670 | 0.819195 | 1.32E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene22670 | 0.871464 | 6.70E-67 | -1.69E-46 |
| MS.gene055750 | MS.gene22670 | 0.86234 | 5.43E-64 | -1.69E-46 |
| MS.gene055751 | MS.gene22670 | 0.892358 | 1.71E-74 | -1.69E-46 |
| MS.gene055776 | MS.gene22670 | 0.919755 | 3.03E-87 | -1.69E-46 |
| MS.gene055849 | MS.gene22670 | 0.911973 | 3.31E-83 | -1.69E-46 |
| MS.gene05589 | MS.gene22670 | 0.835692 | 1.45E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene22670 | 0.814544 | 1.46E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene22670 | -0.801054 | 1.08E-48 | -1.69E-46 |
| MS.gene056046 | MS.gene22670 | 0.813909 | 2.02E-51 | -1.69E-46 |
| MS.gene056083 | MS.gene22670 | 0.805704 | 1.17E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene22670 | 0.839872 | 1.22E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene22670 | 0.880408 | 5.63E-70 | -1.69E-46 |
| MS.gene056510 | MS.gene22670 | 0.899197 | 2.52E-77 | -1.69E-46 |
| MS.gene056549 | MS.gene22670 | 0.837902 | 3.95E-57 | -1.69E-46 |
| MS.gene056657 | MS.gene22670 | 0.908457 | 1.67E-81 | -1.69E-46 |
| MS.gene056692 | MS.gene22670 | 0.853927 | 1.73E-61 | -1.69E-46 |
| MS.gene056898 | MS.gene22670 | 0.835847 | 1.32E-56 | -1.69E-46 |
| MS.gene056910 | MS.gene22670 | 0.87867 | 2.33E-69 | -1.69E-46 |
| MS.gene057129 | MS.gene22670 | 0.800049 | 1.73E-48 | -1.69E-46 |
| MS.gene057148 | MS.gene22670 | 0.823661 | 1.23E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene22670 | 0.808 | 3.84E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene22670 | 0.819257 | 1.28E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene22670 | 0.838878 | 2.21E-57 | -1.69E-46 |
| MS.gene057872 | MS.gene22670 | 0.825376 | 4.85E-54 | -1.69E-46 |
| MS.gene057986 | MS.gene22670 | 0.834797 | 2.43E-56 | -1.69E-46 |
| MS.gene058334 | MS.gene22670 | -0.833147 | 6.30E-56 | -1.69E-46 |
| MS.gene058335 | MS.gene22670 | 0.830139 | 3.47E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene22670 | 0.894108 | 3.36E-75 | -1.69E-46 |
| MS.gene058544 | MS.gene22670 | 0.824876 | 6.36E-54 | -1.69E-46 |
| MS.gene058719 | MS.gene22670 | 0.868105 | 8.36E-66 | -1.69E-46 |
| MS.gene058723 | MS.gene22670 | 0.866957 | 1.95E-65 | -1.69E-46 |
| MS.gene058773 | MS.gene22670 | 0.892531 | 1.46E-74 | -1.69E-46 |
| MS.gene059022 | MS.gene22670 | 0.902038 | 1.46E-78 | -1.69E-46 |
| MS.gene059023 | MS.gene22670 | 0.84253 | 2.44E-58 | -1.69E-46 |
| MS.gene059026 | MS.gene22670 | 0.840735 | 7.27E-58 | -1.69E-46 |
| MS.gene059029 | MS.gene22670 | 0.858024 | 1.09E-62 | -1.69E-46 |
| MS.gene059079 | MS.gene22670 | 0.8633 | 2.75E-64 | -1.69E-46 |
| MS.gene05912 | MS.gene22670 | 0.853701 | 2.01E-61 | -1.69E-46 |
| MS.gene05913 | MS.gene22670 | 0.835282 | 1.84E-56 | -1.69E-46 |
| MS.gene059181 | MS.gene22670 | 0.805165 | 1.52E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene22670 | 0.916265 | 2.19E-85 | -1.69E-46 |
| MS.gene059718 | MS.gene22670 | 0.857278 | 1.82E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene22670 | 0.831745 | 1.40E-55 | -1.69E-46 |
| MS.gene059801 | MS.gene22670 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene059837 | MS.gene22670 | 0.83083 | 2.35E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene22670 | 0.905282 | 5.06E-80 | -1.69E-46 |
| MS.gene059902 | MS.gene22670 | 0.807743 | 4.36E-50 | -1.69E-46 |
| MS.gene060337 | MS.gene22670 | -0.842866 | 1.99E-58 | -1.69E-46 |
| MS.gene060526 | MS.gene22670 | 0.824915 | 6.23E-54 | -1.69E-46 |
| MS.gene060703 | MS.gene22670 | 0.899673 | 1.57E-77 | -1.69E-46 |
| MS.gene060762 | MS.gene22670 | 0.888111 | 7.89E-73 | -1.69E-46 |
| MS.gene060778 | MS.gene22670 | 0.812352 | 4.43E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene22670 | 0.858112 | 1.03E-62 | -1.69E-46 |
| MS.gene060823 | MS.gene22670 | -0.815753 | 7.87E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene22670 | 0.879471 | 1.21E-69 | -1.69E-46 |
| MS.gene061134 | MS.gene22670 | 0.824853 | 6.45E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene22670 | 0.846742 | 1.79E-59 | -1.69E-46 |
| MS.gene061171 | MS.gene22670 | 0.806268 | 8.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22670.t1 | MTR_7g114900 | 99.531 | 213 | 1 | 0 | 1 | 213 | 68 | 280 | 2.63e-153 | 426 |
| MS.gene22670.t1 | MTR_1g108440 | 66.822 | 214 | 70 | 1 | 1 | 213 | 55 | 268 | 5.91e-99 | 288 |
| MS.gene22670.t1 | MTR_2g032540 | 27.111 | 225 | 149 | 5 | 1 | 213 | 67 | 288 | 4.15e-23 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22670.t1 | AT3G04790 | 78.404 | 213 | 46 | 0 | 1 | 213 | 64 | 276 | 2.51e-121 | 345 |
| MS.gene22670.t1 | AT2G01290 | 62.441 | 213 | 80 | 0 | 1 | 213 | 52 | 264 | 1.43e-93 | 275 |
| MS.gene22670.t1 | AT1G71100 | 60.092 | 218 | 82 | 1 | 1 | 213 | 48 | 265 | 4.83e-86 | 255 |
| MS.gene22670.t1 | AT5G44520 | 31.532 | 222 | 136 | 5 | 1 | 209 | 56 | 274 | 1.12e-25 | 101 |
| MS.gene22670.t1 | AT5G44520 | 31.532 | 222 | 136 | 5 | 1 | 209 | 86 | 304 | 2.27e-25 | 101 |
Find 60 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTTCTCAACCTTGTTAA+AGG | 0.149369 | 7.2:-2357017 | MS.gene22670:CDS |
| GTTGAGAAGTTCGCCGAGTT+TGG | 0.275002 | 7.2:+2357181 | None:intergenic |
| TGGTATTCCTCTCTCTGTTC+TGG | 0.303086 | 7.2:-2357090 | MS.gene22670:CDS |
| AGCTTGTTCTTCGGTGCGTT+TGG | 0.324267 | 7.2:+2357121 | None:intergenic |
| TGCTCTCCTCCGTGAGAAAA+TGG | 0.332089 | 7.2:-2356985 | MS.gene22670:CDS |
| GTTGGAAATATAATTTGATT+AGG | 0.342353 | 7.2:-2356857 | MS.gene22670:CDS |
| GTCATCATTGCTGGTAAAAC+TGG | 0.347429 | 7.2:-2356630 | MS.gene22670:CDS |
| AGCAGCATTTGCATCTCTAA+TGG | 0.354451 | 7.2:+2356719 | None:intergenic |
| AGCGGCATGGTCCTCGGCCT+CGG | 0.399773 | 7.2:-2357233 | None:intergenic |
| TTCTTCGGTGCGTTTGGAAG+TGG | 0.409826 | 7.2:+2357127 | None:intergenic |
| GCTACCTCTGTCATCATTGC+TGG | 0.413073 | 7.2:-2356639 | MS.gene22670:CDS |
| GCTGAGATTTCGTCGCTTGA+AGG | 0.433006 | 7.2:-2356693 | MS.gene22670:CDS |
| TTAGAATTATCAGTAACATA+TGG | 0.433220 | 7.2:+2356771 | None:intergenic |
| GACGAAGGCGGCGGTGGAGC+CGG | 0.434905 | 7.2:+2357208 | None:intergenic |
| GCAGCATTTGCATCTCTAAT+GGG | 0.454829 | 7.2:+2356720 | None:intergenic |
| GTCTCTGGTCTCGGTGGAAG+CGG | 0.462851 | 7.2:-2356912 | MS.gene22670:CDS |
| ACGAAACTGGTCTCTGGTCT+CGG | 0.463473 | 7.2:-2356921 | MS.gene22670:CDS |
| CTCAACCTTGTTAAAGGCCG+CGG | 0.475097 | 7.2:-2357011 | MS.gene22670:CDS |
| CCTTTAACAAGGTTGAGAAA+TGG | 0.475449 | 7.2:+2357017 | None:intergenic |
| GGTGGTCGATGATACGAAAC+TGG | 0.476820 | 7.2:-2356934 | MS.gene22670:CDS |
| TGGAGCCGGTGCCGAGGCCG+AGG | 0.476978 | 7.2:+2357222 | None:intergenic |
| GGAGACGACGAAGGCGGCGG+TGG | 0.479468 | 7.2:+2357202 | None:intergenic |
| ATGGTCCTCGGCCTCGGCAC+CGG | 0.489708 | 7.2:-2357227 | MS.gene22670:CDS |
| ATTAGAGATGCAAATGCTGC+TGG | 0.490372 | 7.2:-2356717 | MS.gene22670:CDS |
| CAAGAATTGTTCAAAGAAGA+AGG | 0.494177 | 7.2:-2356831 | MS.gene22670:CDS |
| GAAGAACAAGCTCGTTCTCT+TGG | 0.494473 | 7.2:-2357110 | MS.gene22670:CDS |
| GTCAAAAGCGGCATGGTCCT+CGG | 0.496677 | 7.2:-2357239 | None:intergenic |
| CGGTTTAGCGATGCCGGTGG+AGG | 0.498139 | 7.2:-2356892 | MS.gene22670:CDS |
| AAGAGAACGAGCTTGTTCTT+CGG | 0.503417 | 7.2:+2357112 | None:intergenic |
| CTTGAAGGTGTCGTCGAGCA+TGG | 0.504176 | 7.2:-2356678 | MS.gene22670:CDS |
| TCTTCGGTGCGTTTGGAAGT+GGG | 0.512477 | 7.2:+2357128 | None:intergenic |
| TGGAAGCGGTTTAGCGATGC+CGG | 0.518745 | 7.2:-2356898 | MS.gene22670:CDS |
| GATGATACGAAACTGGTCTC+TGG | 0.522213 | 7.2:-2356927 | MS.gene22670:CDS |
| CACCGCCGCGGCCTTTAACA+AGG | 0.522675 | 7.2:+2357006 | None:intergenic |
| GCATGGTTTGTTCTTGAACA+TGG | 0.523331 | 7.2:-2356661 | MS.gene22670:CDS |
| CGTCTCGATCTCGCCATCGA+CGG | 0.527285 | 7.2:-2357056 | MS.gene22670:CDS |
| CGGCGGTGGAGCCGGTGCCG+AGG | 0.537408 | 7.2:+2357216 | None:intergenic |
| GATGATGTTGGTGAGTTCGC+CGG | 0.539899 | 7.2:+2357157 | None:intergenic |
| GCCTTCGTCGTCTCCAAACT+CGG | 0.546205 | 7.2:-2357194 | MS.gene22670:CDS |
| GGCGAACTCACCAACATCAT+CGG | 0.560538 | 7.2:-2357155 | MS.gene22670:CDS |
| CAAAACTGAACAACCTCCAC+CGG | 0.562915 | 7.2:+2356879 | None:intergenic |
| TTTGGAGACGACGAAGGCGG+CGG | 0.566766 | 7.2:+2357199 | None:intergenic |
| CACGACGAATTTATCAGAAG+CGG | 0.584428 | 7.2:+2356956 | None:intergenic |
| TTATCGTCCAGAACAGAGAG+AGG | 0.606235 | 7.2:+2357083 | None:intergenic |
| TTCTGATAAATTCGTCGTGG+TGG | 0.606783 | 7.2:-2356952 | MS.gene22670:CDS |
| GACTTCATCAGCACCGTCGA+TGG | 0.609689 | 7.2:+2357043 | None:intergenic |
| AGTGGGAACGCCGATGATGT+TGG | 0.637767 | 7.2:+2357145 | None:intergenic |
| AAGCGGTTTAGCGATGCCGG+TGG | 0.640481 | 7.2:-2356895 | MS.gene22670:CDS |
| GCCGAGTTTGGAGACGACGA+AGG | 0.645857 | 7.2:+2357193 | None:intergenic |
| CGTTGATGCGAAATTGAGAG+TGG | 0.649594 | 7.2:-2356808 | MS.gene22670:CDS |
| AAATTGAGAGTGGATGAAAG+TGG | 0.649838 | 7.2:-2356798 | MS.gene22670:CDS |
| GAGTTTGGAGACGACGAAGG+CGG | 0.656291 | 7.2:+2357196 | None:intergenic |
| CTCGGCGAACTTCTCAACTC+CGG | 0.656946 | 7.2:-2357176 | MS.gene22670:CDS |
| CGCTTCTGATAAATTCGTCG+TGG | 0.657924 | 7.2:-2356955 | MS.gene22670:CDS |
| AACCTTGTTAAAGGCCGCGG+CGG | 0.661052 | 7.2:-2357008 | MS.gene22670:CDS |
| CACGGAGGAGAGCACCGCCG+CGG | 0.668975 | 7.2:+2356994 | None:intergenic |
| AAACTGGTCTCTGGTCTCGG+TGG | 0.677817 | 7.2:-2356918 | MS.gene22670:CDS |
| TTTACCAGCAATGATGACAG+AGG | 0.680041 | 7.2:+2356635 | None:intergenic |
| TCTCCTCCGTGAGAAAATGG+TGG | 0.693512 | 7.2:-2356982 | MS.gene22670:CDS |
| TCGATGGCGAGATCGAGACG+AGG | 0.700409 | 7.2:+2357059 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGGAAATATAATTTGATT+AGG | - | chr7.2:2356978-2356997 | MS.gene22670:CDS | 20.0% |
| !! | TTAGAATTATCAGTAACATA+TGG | + | chr7.2:2357067-2357086 | None:intergenic | 20.0% |
| CAAGAATTGTTCAAAGAAGA+AGG | - | chr7.2:2357004-2357023 | MS.gene22670:CDS | 30.0% | |
| AAATTGAGAGTGGATGAAAG+TGG | - | chr7.2:2357037-2357056 | MS.gene22670:CDS | 35.0% | |
| CCATTTCTCAACCTTGTTAA+AGG | - | chr7.2:2356818-2356837 | MS.gene22670:CDS | 35.0% | |
| CCTTTAACAAGGTTGAGAAA+TGG | + | chr7.2:2356821-2356840 | None:intergenic | 35.0% | |
| AGCAGCATTTGCATCTCTAA+TGG | + | chr7.2:2357119-2357138 | None:intergenic | 40.0% | |
| ATTAGAGATGCAAATGCTGC+TGG | - | chr7.2:2357118-2357137 | MS.gene22670:CDS | 40.0% | |
| CACGACGAATTTATCAGAAG+CGG | + | chr7.2:2356882-2356901 | None:intergenic | 40.0% | |
| GCAGCATTTGCATCTCTAAT+GGG | + | chr7.2:2357118-2357137 | None:intergenic | 40.0% | |
| GTCATCATTGCTGGTAAAAC+TGG | - | chr7.2:2357205-2357224 | MS.gene22670:CDS | 40.0% | |
| TTCTGATAAATTCGTCGTGG+TGG | - | chr7.2:2356883-2356902 | MS.gene22670:CDS | 40.0% | |
| TTTACCAGCAATGATGACAG+AGG | + | chr7.2:2357203-2357222 | None:intergenic | 40.0% | |
| ! | AAGAGAACGAGCTTGTTCTT+CGG | + | chr7.2:2356726-2356745 | None:intergenic | 40.0% |
| !! | GCATGGTTTGTTCTTGAACA+TGG | - | chr7.2:2357174-2357193 | MS.gene22670:CDS | 40.0% |
| !! | TGGAGGTTGTTCAGTTTTGT+TGG | - | chr7.2:2356960-2356979 | MS.gene22670:CDS | 40.0% |
| CAAAACTGAACAACCTCCAC+CGG | + | chr7.2:2356959-2356978 | None:intergenic | 45.0% | |
| CGCTTCTGATAAATTCGTCG+TGG | - | chr7.2:2356880-2356899 | MS.gene22670:CDS | 45.0% | |
| CGTTGATGCGAAATTGAGAG+TGG | - | chr7.2:2357027-2357046 | MS.gene22670:CDS | 45.0% | |
| GAAGAACAAGCTCGTTCTCT+TGG | - | chr7.2:2356725-2356744 | MS.gene22670:CDS | 45.0% | |
| TGGTATTCCTCTCTCTGTTC+TGG | - | chr7.2:2356745-2356764 | MS.gene22670:CDS | 45.0% | |
| TTATCGTCCAGAACAGAGAG+AGG | + | chr7.2:2356755-2356774 | None:intergenic | 45.0% | |
| ! | GATGATACGAAACTGGTCTC+TGG | - | chr7.2:2356908-2356927 | MS.gene22670:CDS | 45.0% |
| CTCAACCTTGTTAAAGGCCG+CGG | - | chr7.2:2356824-2356843 | MS.gene22670:CDS | 50.0% | |
| GCTACCTCTGTCATCATTGC+TGG | - | chr7.2:2357196-2357215 | MS.gene22670:CDS | 50.0% | |
| GCTGAGATTTCGTCGCTTGA+AGG | - | chr7.2:2357142-2357161 | MS.gene22670:CDS | 50.0% | |
| GGCGAACTCACCAACATCAT+CGG | - | chr7.2:2356680-2356699 | MS.gene22670:CDS | 50.0% | |
| GGTGGTCGATGATACGAAAC+TGG | - | chr7.2:2356901-2356920 | MS.gene22670:CDS | 50.0% | |
| GTTGAGAAGTTCGCCGAGTT+TGG | + | chr7.2:2356657-2356676 | None:intergenic | 50.0% | |
| TCTCCTCCGTGAGAAAATGG+TGG | - | chr7.2:2356853-2356872 | MS.gene22670:CDS | 50.0% | |
| TGCTCTCCTCCGTGAGAAAA+TGG | - | chr7.2:2356850-2356869 | MS.gene22670:CDS | 50.0% | |
| ! | ACGAAACTGGTCTCTGGTCT+CGG | - | chr7.2:2356914-2356933 | MS.gene22670:CDS | 50.0% |
| ! | AGCTTGTTCTTCGGTGCGTT+TGG | + | chr7.2:2356717-2356736 | None:intergenic | 50.0% |
| ! | CGGCTTCCACCATTTTCTCA+CGG | + | chr7.2:2356862-2356881 | None:intergenic | 50.0% |
| ! | CTTCCACCATTTTCTCACGG+AGG | + | chr7.2:2356859-2356878 | None:intergenic | 50.0% |
| ! | GATGATGTTGGTGAGTTCGC+CGG | + | chr7.2:2356681-2356700 | None:intergenic | 50.0% |
| ! | TCTTCGGTGCGTTTGGAAGT+GGG | + | chr7.2:2356710-2356729 | None:intergenic | 50.0% |
| ! | TTCTTCGGTGCGTTTGGAAG+TGG | + | chr7.2:2356711-2356730 | None:intergenic | 50.0% |
| AACCTTGTTAAAGGCCGCGG+CGG | - | chr7.2:2356827-2356846 | MS.gene22670:CDS | 55.0% | |
| AGTGGGAACGCCGATGATGT+TGG | + | chr7.2:2356693-2356712 | None:intergenic | 55.0% | |
| CTCGGCGAACTTCTCAACTC+CGG | - | chr7.2:2356659-2356678 | MS.gene22670:CDS | 55.0% | |
| GACTTCATCAGCACCGTCGA+TGG | + | chr7.2:2356795-2356814 | None:intergenic | 55.0% | |
| GAGTTTGGAGACGACGAAGG+CGG | + | chr7.2:2356642-2356661 | None:intergenic | 55.0% | |
| GCCTTCGTCGTCTCCAAACT+CGG | - | chr7.2:2356641-2356660 | MS.gene22670:CDS | 55.0% | |
| TGGAAGCGGTTTAGCGATGC+CGG | - | chr7.2:2356937-2356956 | MS.gene22670:CDS | 55.0% | |
| ! | AAACTGGTCTCTGGTCTCGG+TGG | - | chr7.2:2356917-2356936 | MS.gene22670:CDS | 55.0% |
| !! | CTTGAAGGTGTCGTCGAGCA+TGG | - | chr7.2:2357157-2357176 | MS.gene22670:CDS | 55.0% |
| AAGCGGTTTAGCGATGCCGG+TGG | - | chr7.2:2356940-2356959 | MS.gene22670:CDS | 60.0% | |
| CGTCTCGATCTCGCCATCGA+CGG | - | chr7.2:2356779-2356798 | MS.gene22670:CDS | 60.0% | |
| GCCGAGTTTGGAGACGACGA+AGG | + | chr7.2:2356645-2356664 | None:intergenic | 60.0% | |
| GTCTCTGGTCTCGGTGGAAG+CGG | - | chr7.2:2356923-2356942 | MS.gene22670:CDS | 60.0% | |
| TCGATGGCGAGATCGAGACG+AGG | + | chr7.2:2356779-2356798 | None:intergenic | 60.0% | |
| TTTGGAGACGACGAAGGCGG+CGG | + | chr7.2:2356639-2356658 | None:intergenic | 60.0% | |
| CACCGCCGCGGCCTTTAACA+AGG | + | chr7.2:2356832-2356851 | None:intergenic | 65.0% | |
| CGGTTTAGCGATGCCGGTGG+AGG | - | chr7.2:2356943-2356962 | MS.gene22670:CDS | 65.0% | |
| ATGGTCCTCGGCCTCGGCAC+CGG | - | chr7.2:2356608-2356627 | MS.gene22670:CDS | 70.0% | |
| GACGAAGGCGGCGGTGGAGC+CGG | + | chr7.2:2356630-2356649 | None:intergenic | 75.0% | |
| GGAGACGACGAAGGCGGCGG+TGG | + | chr7.2:2356636-2356655 | None:intergenic | 75.0% | |
| !! | CACGGAGGAGAGCACCGCCG+CGG | + | chr7.2:2356844-2356863 | None:intergenic | 75.0% |
| !! | TGGAGCCGGTGCCGAGGCCG+AGG | + | chr7.2:2356616-2356635 | None:intergenic | 80.0% |
| !! | CGGCGGTGGAGCCGGTGCCG+AGG | + | chr7.2:2356622-2356641 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 2356608 | 2357249 | 2356608 | ID=MS.gene22670 |
| chr7.2 | mRNA | 2356608 | 2357249 | 2356608 | ID=MS.gene22670.t1;Parent=MS.gene22670 |
| chr7.2 | exon | 2356608 | 2357249 | 2356608 | ID=MS.gene22670.t1.exon1;Parent=MS.gene22670.t1 |
| chr7.2 | CDS | 2356608 | 2357249 | 2356608 | ID=cds.MS.gene22670.t1;Parent=MS.gene22670.t1 |
| Gene Sequence |
| Protein sequence |
