Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23403.t1 | XP_003627712.3 | 88.8 | 89 | 10 | 0 | 12 | 100 | 17 | 105 | 6.20E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23403.t1 | A0A072TPC7 | 86.1 | 72 | 10 | 0 | 29 | 100 | 1 | 72 | 7.1e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene23403 | 0.848001 | 8.09E-60 | -1.69E-46 |
| MS.gene049171 | MS.gene23403 | 0.832603 | 8.60E-56 | -1.69E-46 |
| MS.gene049173 | MS.gene23403 | 0.812429 | 4.26E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene23403 | 0.818357 | 2.04E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene23403 | 0.858852 | 6.20E-63 | -1.69E-46 |
| MS.gene049285 | MS.gene23403 | 0.840329 | 9.29E-58 | -1.69E-46 |
| MS.gene049320 | MS.gene23403 | 0.832528 | 8.98E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene23403 | 0.853745 | 1.95E-61 | -1.69E-46 |
| MS.gene049805 | MS.gene23403 | 0.820696 | 5.98E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene23403 | 0.825084 | 5.68E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene23403 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene23403 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene050323 | MS.gene23403 | 0.804789 | 1.82E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene23403 | 0.841127 | 5.74E-58 | -1.69E-46 |
| MS.gene050507 | MS.gene23403 | 0.822725 | 2.03E-53 | -1.69E-46 |
| MS.gene050518 | MS.gene23403 | 0.825967 | 3.51E-54 | -1.69E-46 |
| MS.gene050520 | MS.gene23403 | 0.808533 | 2.96E-50 | -1.69E-46 |
| MS.gene050538 | MS.gene23403 | 0.839317 | 1.70E-57 | -1.69E-46 |
| MS.gene050577 | MS.gene23403 | 0.840589 | 7.94E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene23403 | 0.803485 | 3.41E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene23403 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene23403 | 0.833986 | 3.89E-56 | -1.69E-46 |
| MS.gene050747 | MS.gene23403 | 0.871878 | 4.89E-67 | -1.69E-46 |
| MS.gene050772 | MS.gene23403 | 0.841016 | 6.13E-58 | -1.69E-46 |
| MS.gene050781 | MS.gene23403 | 0.829033 | 6.45E-55 | -1.69E-46 |
| MS.gene050783 | MS.gene23403 | 0.807224 | 5.61E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene23403 | 0.828933 | 6.83E-55 | -1.69E-46 |
| MS.gene050812 | MS.gene23403 | 0.800708 | 1.27E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene23403 | 0.813216 | 2.86E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene23403 | 0.813814 | 2.12E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene23403 | 0.858658 | 7.08E-63 | -1.69E-46 |
| MS.gene050986 | MS.gene23403 | 0.824568 | 7.52E-54 | -1.69E-46 |
| MS.gene05100 | MS.gene23403 | 0.831149 | 1.97E-55 | -1.69E-46 |
| MS.gene05105 | MS.gene23403 | 0.828167 | 1.05E-54 | -1.69E-46 |
| MS.gene051387 | MS.gene23403 | 0.810685 | 1.02E-50 | -1.69E-46 |
| MS.gene051438 | MS.gene23403 | 0.811739 | 6.02E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene23403 | 0.832566 | 8.79E-56 | -1.69E-46 |
| MS.gene051666 | MS.gene23403 | 0.847901 | 8.62E-60 | -1.69E-46 |
| MS.gene051734 | MS.gene23403 | 0.8159 | 7.29E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene23403 | 0.824661 | 7.15E-54 | -1.69E-46 |
| MS.gene051941 | MS.gene23403 | 0.802629 | 5.12E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene23403 | 0.866939 | 1.98E-65 | -1.69E-46 |
| MS.gene052414 | MS.gene23403 | 0.814267 | 1.68E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene23403 | 0.844947 | 5.51E-59 | -1.69E-46 |
| MS.gene052482 | MS.gene23403 | 0.814249 | 1.70E-51 | -1.69E-46 |
| MS.gene052486 | MS.gene23403 | 0.814003 | 1.92E-51 | -1.69E-46 |
| MS.gene052501 | MS.gene23403 | 0.835903 | 1.28E-56 | -1.69E-46 |
| MS.gene052503 | MS.gene23403 | 0.858628 | 7.23E-63 | -1.69E-46 |
| MS.gene052505 | MS.gene23403 | 0.824668 | 7.12E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene23403 | 0.822403 | 2.41E-53 | -1.69E-46 |
| MS.gene052823 | MS.gene23403 | 0.854713 | 1.02E-61 | -1.69E-46 |
| MS.gene052825 | MS.gene23403 | 0.850371 | 1.77E-60 | -1.69E-46 |
| MS.gene052966 | MS.gene23403 | 0.805326 | 1.41E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene23403 | 0.83208 | 1.16E-55 | -1.69E-46 |
| MS.gene053203 | MS.gene23403 | 0.843655 | 1.23E-58 | -1.69E-46 |
| MS.gene053207 | MS.gene23403 | 0.848597 | 5.54E-60 | -1.69E-46 |
| MS.gene053213 | MS.gene23403 | 0.849672 | 2.78E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene23403 | 0.847981 | 8.19E-60 | -1.69E-46 |
| MS.gene053436 | MS.gene23403 | 0.845506 | 3.89E-59 | -1.69E-46 |
| MS.gene053480 | MS.gene23403 | 0.837288 | 5.68E-57 | -1.69E-46 |
| MS.gene053574 | MS.gene23403 | 0.85362 | 2.12E-61 | -1.69E-46 |
| MS.gene053579 | MS.gene23403 | 0.811765 | 5.94E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene23403 | 0.843569 | 1.29E-58 | -1.69E-46 |
| MS.gene053632 | MS.gene23403 | 0.858252 | 9.36E-63 | -1.69E-46 |
| MS.gene053824 | MS.gene23403 | 0.833023 | 6.77E-56 | -1.69E-46 |
| MS.gene053916 | MS.gene23403 | 0.819655 | 1.04E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene23403 | 0.834032 | 3.79E-56 | -1.69E-46 |
| MS.gene054595 | MS.gene23403 | 0.833696 | 4.60E-56 | -1.69E-46 |
| MS.gene054600 | MS.gene23403 | 0.844624 | 6.74E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene23403 | 0.817245 | 3.64E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene23403 | 0.834931 | 2.25E-56 | -1.69E-46 |
| MS.gene054927 | MS.gene23403 | 0.858019 | 1.10E-62 | -1.69E-46 |
| MS.gene054954 | MS.gene23403 | 0.815882 | 7.36E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene23403 | 0.843821 | 1.11E-58 | -1.69E-46 |
| MS.gene055206 | MS.gene23403 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene055212 | MS.gene23403 | 0.80315 | 4.00E-49 | -1.69E-46 |
| MS.gene055224 | MS.gene23403 | 0.817652 | 2.95E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene23403 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene23403 | 0.840244 | 9.77E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene23403 | 0.840547 | 8.14E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene23403 | 0.841422 | 4.80E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene23403 | 0.820967 | 5.18E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene23403 | 0.819847 | 9.37E-53 | -1.69E-46 |
| MS.gene055750 | MS.gene23403 | 0.83245 | 9.39E-56 | -1.69E-46 |
| MS.gene055751 | MS.gene23403 | 0.806679 | 7.32E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene23403 | 0.813046 | 3.12E-51 | -1.69E-46 |
| MS.gene055941 | MS.gene23403 | 0.817976 | 2.49E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene23403 | 0.838918 | 2.16E-57 | -1.69E-46 |
| MS.gene056166 | MS.gene23403 | 0.817548 | 3.12E-52 | -1.69E-46 |
| MS.gene056409 | MS.gene23403 | 0.811437 | 7.00E-51 | -1.69E-46 |
| MS.gene056449 | MS.gene23403 | 0.838094 | 3.53E-57 | -1.69E-46 |
| MS.gene056487 | MS.gene23403 | 0.808841 | 2.54E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene23403 | 0.828659 | 7.95E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene23403 | 0.819479 | 1.14E-52 | -1.69E-46 |
| MS.gene056610 | MS.gene23403 | 0.801903 | 7.23E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene23403 | 0.844782 | 6.11E-59 | -1.69E-46 |
| MS.gene056692 | MS.gene23403 | 0.817401 | 3.36E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene23403 | 0.85149 | 8.58E-61 | -1.69E-46 |
| MS.gene057076 | MS.gene23403 | 0.805666 | 1.20E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene23403 | 0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene057658 | MS.gene23403 | 0.873374 | 1.55E-67 | -1.69E-46 |
| MS.gene057831 | MS.gene23403 | 0.82628 | 2.96E-54 | -1.69E-46 |
| MS.gene05787 | MS.gene23403 | 0.842568 | 2.39E-58 | -1.69E-46 |
| MS.gene058019 | MS.gene23403 | 0.844009 | 9.86E-59 | -1.69E-46 |
| MS.gene058120 | MS.gene23403 | 0.80744 | 5.05E-50 | -1.69E-46 |
| MS.gene058130 | MS.gene23403 | 0.807593 | 4.69E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene23403 | 0.819176 | 1.33E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene23403 | 0.822005 | 2.98E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene23403 | 0.844879 | 5.75E-59 | -1.69E-46 |
| MS.gene058544 | MS.gene23403 | 0.84043 | 8.74E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene23403 | 0.805137 | 1.54E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene23403 | 0.818463 | 1.93E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene23403 | 0.84117 | 5.59E-58 | -1.69E-46 |
| MS.gene058948 | MS.gene23403 | 0.821045 | 4.97E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene23403 | 0.814504 | 1.49E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene23403 | 0.843086 | 1.74E-58 | -1.69E-46 |
| MS.gene05913 | MS.gene23403 | 0.806222 | 9.13E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene23403 | 0.850934 | 1.23E-60 | -1.69E-46 |
| MS.gene059367 | MS.gene23403 | 0.831059 | 2.07E-55 | -1.69E-46 |
| MS.gene059667 | MS.gene23403 | 0.848976 | 4.35E-60 | -1.69E-46 |
| MS.gene059677 | MS.gene23403 | 0.841018 | 6.13E-58 | -1.69E-46 |
| MS.gene059680 | MS.gene23403 | 0.802736 | 4.87E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene23403 | 0.870423 | 1.48E-66 | -1.69E-46 |
| MS.gene059756 | MS.gene23403 | 0.837293 | 5.66E-57 | -1.69E-46 |
| MS.gene059801 | MS.gene23403 | 0.809899 | 1.51E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene23403 | 0.80149 | 8.79E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene23403 | 0.829496 | 4.98E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene23403 | 0.815002 | 1.16E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene23403 | 0.838712 | 2.44E-57 | -1.69E-46 |
| MS.gene060835 | MS.gene23403 | 0.845369 | 4.24E-59 | -1.69E-46 |
| MS.gene06092 | MS.gene23403 | 0.854114 | 1.53E-61 | -1.69E-46 |
| MS.gene061041 | MS.gene23403 | 0.81263 | 3.85E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene23403 | 0.838886 | 2.20E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene23403 | 0.809617 | 1.73E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23403.t1 | MTR_8g036830 | 86.111 | 72 | 10 | 0 | 29 | 100 | 1 | 72 | 1.80e-42 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCTTCTTGTGTTAGAAAT+TGG | 0.117758 | 8.1:+66475527 | None:intergenic |
| CCATGAACATGGGATGAATA+TGG | 0.288739 | 8.1:+66475455 | None:intergenic |
| AATATGGCTTGAAGAAAATA+AGG | 0.291272 | 8.1:+66475471 | None:intergenic |
| GCTTGAAGAAAATAAGGTTT+GGG | 0.338713 | 8.1:+66475477 | None:intergenic |
| GGCTTGAAGAAAATAAGGTT+TGG | 0.392638 | 8.1:+66475476 | None:intergenic |
| TAACACAAGAAGAATCATAA+TGG | 0.400261 | 8.1:-66475519 | MS.gene23403:CDS |
| CCATGTTCATGGAAATTTAG+AGG | 0.404305 | 8.1:-66475444 | MS.gene23403:CDS |
| CCATATTCATCCCATGTTCA+TGG | 0.415143 | 8.1:-66475455 | MS.gene23403:CDS |
| TTCTTCTTGTGTTAGAAATT+GGG | 0.416058 | 8.1:+66475528 | None:intergenic |
| CCACTTTCTTGCTCTTTCCA+CGG | 0.452311 | 8.1:-66475317 | MS.gene23403:CDS |
| CCTCTAAATTTCCATGAACA+TGG | 0.482444 | 8.1:+66475444 | None:intergenic |
| TCTTTCCACGGTCATACAAA+TGG | 0.498540 | 8.1:-66475305 | None:intergenic |
| CTTGTGTTAGAAATTGGGAA+GGG | 0.553869 | 8.1:+66475533 | None:intergenic |
| CTAATGTTCAAACAAAGAAA+CGG | 0.561468 | 8.1:-66475382 | MS.gene23403:CDS |
| CCGTGGAAAGAGCAAGAAAG+TGG | 0.561979 | 8.1:+66475317 | None:intergenic |
| TGTGTTAGAAATTGGGAAGG+GGG | 0.568484 | 8.1:+66475535 | None:intergenic |
| TGAGAGAAAGATGATAACGT+TGG | 0.576181 | 8.1:+66475581 | None:intergenic |
| TTGTGTTAGAAATTGGGAAG+GGG | 0.578970 | 8.1:+66475534 | None:intergenic |
| CTCTAAATTTCCATGAACAT+GGG | 0.587547 | 8.1:+66475445 | None:intergenic |
| TCTTGTGTTAGAAATTGGGA+AGG | 0.595691 | 8.1:+66475532 | None:intergenic |
| CTTGAAGAAAATAAGGTTTG+GGG | 0.597299 | 8.1:+66475478 | None:intergenic |
| GCATACCATTTGTATGACCG+TGG | 0.672386 | 8.1:+66475300 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATGGCTTGAAGAAAATA+AGG | + | chr8.1:66475422-66475441 | None:intergenic | 25.0% |
| ! | ATTCTTCTTGTGTTAGAAAT+TGG | + | chr8.1:66475366-66475385 | None:intergenic | 25.0% |
| ! | CTAATGTTCAAACAAAGAAA+CGG | - | chr8.1:66475508-66475527 | MS.gene23403:CDS | 25.0% |
| ! | TAACACAAGAAGAATCATAA+TGG | - | chr8.1:66475371-66475390 | MS.gene23403:CDS | 25.0% |
| ! | TTCTTCTTGTGTTAGAAATT+GGG | + | chr8.1:66475365-66475384 | None:intergenic | 25.0% |
| CTCTAAATTTCCATGAACAT+GGG | + | chr8.1:66475448-66475467 | None:intergenic | 30.0% | |
| CTTGAAGAAAATAAGGTTTG+GGG | + | chr8.1:66475415-66475434 | None:intergenic | 30.0% | |
| GCTTGAAGAAAATAAGGTTT+GGG | + | chr8.1:66475416-66475435 | None:intergenic | 30.0% | |
| ! | CATAAGAGAAGAACTTTTGT+TGG | - | chr8.1:66475330-66475349 | MS.gene23403:CDS | 30.0% |
| CCATGTTCATGGAAATTTAG+AGG | - | chr8.1:66475446-66475465 | MS.gene23403:CDS | 35.0% | |
| CCTCTAAATTTCCATGAACA+TGG | + | chr8.1:66475449-66475468 | None:intergenic | 35.0% | |
| CTTGTGTTAGAAATTGGGAA+GGG | + | chr8.1:66475360-66475379 | None:intergenic | 35.0% | |
| GGCTTGAAGAAAATAAGGTT+TGG | + | chr8.1:66475417-66475436 | None:intergenic | 35.0% | |
| TCTTGTGTTAGAAATTGGGA+AGG | + | chr8.1:66475361-66475380 | None:intergenic | 35.0% | |
| TTGTGTTAGAAATTGGGAAG+GGG | + | chr8.1:66475359-66475378 | None:intergenic | 35.0% | |
| ! | TGAGAGAAAGATGATAACGT+TGG | + | chr8.1:66475312-66475331 | None:intergenic | 35.0% |
| CCATATTCATCCCATGTTCA+TGG | - | chr8.1:66475435-66475454 | MS.gene23403:CDS | 40.0% | |
| CCATGAACATGGGATGAATA+TGG | + | chr8.1:66475438-66475457 | None:intergenic | 40.0% | |
| TGTGTTAGAAATTGGGAAGG+GGG | + | chr8.1:66475358-66475377 | None:intergenic | 40.0% | |
| CCACTTTCTTGCTCTTTCCA+CGG | - | chr8.1:66475573-66475592 | MS.gene23403:CDS | 45.0% | |
| CCGTGGAAAGAGCAAGAAAG+TGG | + | chr8.1:66475576-66475595 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 66475306 | 66475606 | 66475306 | ID=MS.gene23403 |
| chr8.1 | mRNA | 66475306 | 66475606 | 66475306 | ID=MS.gene23403.t1;Parent=MS.gene23403 |
| chr8.1 | exon | 66475306 | 66475606 | 66475306 | ID=MS.gene23403.t1.exon1;Parent=MS.gene23403.t1 |
| chr8.1 | CDS | 66475306 | 66475606 | 66475306 | ID=cds.MS.gene23403.t1;Parent=MS.gene23403.t1 |
| Gene Sequence |
| Protein sequence |
