Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38351.t1 | XP_003603045.2 | 89.5 | 162 | 11 | 1 | 1 | 156 | 57 | 218 | 1.30E-75 | 292.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38351.t1 | Q9C5C5 | 76.0 | 129 | 31 | 0 | 23 | 151 | 76 | 204 | 4.1e-54 | 212.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38351.t1 | G7J6D9 | 89.5 | 162 | 11 | 1 | 1 | 156 | 57 | 218 | 9.3e-76 | 292.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene38351 | 0.823445 | 1.38E-53 | -1.69E-46 |
| MS.gene049284 | MS.gene38351 | 0.804684 | 1.92E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene38351 | 0.813691 | 2.25E-51 | -1.69E-46 |
| MS.gene049351 | MS.gene38351 | 0.856011 | 4.29E-62 | -1.69E-46 |
| MS.gene049427 | MS.gene38351 | 0.833189 | 6.15E-56 | -1.69E-46 |
| MS.gene049491 | MS.gene38351 | 0.804294 | 2.32E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene38351 | 0.802916 | 4.47E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene38351 | 0.884141 | 2.47E-71 | -1.69E-46 |
| MS.gene049662 | MS.gene38351 | 0.88284 | 7.43E-71 | -1.69E-46 |
| MS.gene049758 | MS.gene38351 | 0.846736 | 1.80E-59 | -1.69E-46 |
| MS.gene049760 | MS.gene38351 | 0.816628 | 5.01E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene38351 | 0.820784 | 5.71E-53 | -1.69E-46 |
| MS.gene050076 | MS.gene38351 | 0.860503 | 1.97E-63 | -1.69E-46 |
| MS.gene050301 | MS.gene38351 | 0.860733 | 1.68E-63 | -1.69E-46 |
| MS.gene050397 | MS.gene38351 | 0.900827 | 4.96E-78 | -1.69E-46 |
| MS.gene050404 | MS.gene38351 | 0.843066 | 1.76E-58 | -1.69E-46 |
| MS.gene050406 | MS.gene38351 | 0.856421 | 3.25E-62 | -1.69E-46 |
| MS.gene050426 | MS.gene38351 | -0.805557 | 1.26E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene38351 | 0.85472 | 1.02E-61 | -1.69E-46 |
| MS.gene050464 | MS.gene38351 | 0.850163 | 2.03E-60 | -1.69E-46 |
| MS.gene050529 | MS.gene38351 | 0.885534 | 7.49E-72 | -1.69E-46 |
| MS.gene050593 | MS.gene38351 | 0.805262 | 1.45E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene38351 | 0.855359 | 6.65E-62 | -1.69E-46 |
| MS.gene050767 | MS.gene38351 | 0.812547 | 4.01E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene38351 | 0.818844 | 1.59E-52 | -1.69E-46 |
| MS.gene050812 | MS.gene38351 | 0.819378 | 1.20E-52 | -1.69E-46 |
| MS.gene050971 | MS.gene38351 | 0.803484 | 3.41E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene38351 | 0.857899 | 1.19E-62 | -1.69E-46 |
| MS.gene05105 | MS.gene38351 | 0.859951 | 2.90E-63 | -1.69E-46 |
| MS.gene051387 | MS.gene38351 | 0.804903 | 1.73E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene38351 | 0.866306 | 3.14E-65 | -1.69E-46 |
| MS.gene051525 | MS.gene38351 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene051582 | MS.gene38351 | 0.836949 | 6.93E-57 | -1.69E-46 |
| MS.gene051666 | MS.gene38351 | 0.829307 | 5.54E-55 | -1.69E-46 |
| MS.gene051692 | MS.gene38351 | 0.803346 | 3.64E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene38351 | 0.805318 | 1.41E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene38351 | 0.806936 | 6.46E-50 | -1.69E-46 |
| MS.gene051877 | MS.gene38351 | 0.824642 | 7.23E-54 | -1.69E-46 |
| MS.gene052011 | MS.gene38351 | -0.808362 | 3.21E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene38351 | 0.834975 | 2.20E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene38351 | 0.880534 | 5.07E-70 | -1.69E-46 |
| MS.gene052178 | MS.gene38351 | 0.831302 | 1.80E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene38351 | 0.85067 | 1.46E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene38351 | 0.871051 | 9.18E-67 | -1.69E-46 |
| MS.gene052290 | MS.gene38351 | 0.828468 | 8.84E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene38351 | 0.905703 | 3.24E-80 | -1.69E-46 |
| MS.gene052322 | MS.gene38351 | 0.87019 | 1.76E-66 | -1.69E-46 |
| MS.gene052326 | MS.gene38351 | 0.84549 | 3.93E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene38351 | 0.873113 | 1.89E-67 | -1.69E-46 |
| MS.gene052379 | MS.gene38351 | 0.807461 | 5.00E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene38351 | 0.839814 | 1.27E-57 | -1.69E-46 |
| MS.gene052481 | MS.gene38351 | 0.847022 | 1.50E-59 | -1.69E-46 |
| MS.gene052484 | MS.gene38351 | 0.824586 | 7.45E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene38351 | 0.843044 | 1.78E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene38351 | 0.85517 | 7.55E-62 | -1.69E-46 |
| MS.gene053104 | MS.gene38351 | 0.828082 | 1.10E-54 | -1.69E-46 |
| MS.gene053107 | MS.gene38351 | 0.81381 | 2.12E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene38351 | 0.868833 | 4.87E-66 | -1.69E-46 |
| MS.gene053322 | MS.gene38351 | 0.836829 | 7.44E-57 | -1.69E-46 |
| MS.gene053557 | MS.gene38351 | 0.852386 | 4.78E-61 | -1.69E-46 |
| MS.gene053836 | MS.gene38351 | 0.861361 | 1.08E-63 | -1.69E-46 |
| MS.gene053928 | MS.gene38351 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene053971 | MS.gene38351 | 0.836543 | 8.80E-57 | -1.69E-46 |
| MS.gene054123 | MS.gene38351 | 0.84146 | 4.69E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene38351 | 0.807726 | 4.39E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene38351 | 0.821936 | 3.10E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene38351 | 0.826309 | 2.91E-54 | -1.69E-46 |
| MS.gene054803 | MS.gene38351 | 0.823374 | 1.43E-53 | -1.69E-46 |
| MS.gene054804 | MS.gene38351 | 0.826086 | 3.29E-54 | -1.69E-46 |
| MS.gene054955 | MS.gene38351 | 0.829916 | 3.94E-55 | -1.69E-46 |
| MS.gene054983 | MS.gene38351 | 0.80976 | 1.61E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene38351 | 0.834157 | 3.53E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene38351 | 0.872274 | 3.61E-67 | -1.69E-46 |
| MS.gene055206 | MS.gene38351 | 0.817969 | 2.50E-52 | -1.69E-46 |
| MS.gene055254 | MS.gene38351 | 0.816906 | 4.34E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene38351 | 0.89348 | 6.05E-75 | -1.69E-46 |
| MS.gene055289 | MS.gene38351 | 0.807021 | 6.19E-50 | -1.69E-46 |
| MS.gene055370 | MS.gene38351 | 0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene055390 | MS.gene38351 | 0.812311 | 4.52E-51 | -1.69E-46 |
| MS.gene055397 | MS.gene38351 | 0.801334 | 9.46E-49 | -1.69E-46 |
| MS.gene055412 | MS.gene38351 | 0.822858 | 1.89E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene38351 | 0.869677 | 2.59E-66 | -1.69E-46 |
| MS.gene055428 | MS.gene38351 | 0.877451 | 6.22E-69 | -1.69E-46 |
| MS.gene055499 | MS.gene38351 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene38351 | 0.823146 | 1.62E-53 | -1.69E-46 |
| MS.gene055896 | MS.gene38351 | 0.812933 | 3.30E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene38351 | 0.821265 | 4.43E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene38351 | 0.838052 | 3.61E-57 | -1.69E-46 |
| MS.gene056314 | MS.gene38351 | 0.801947 | 7.08E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene38351 | 0.845444 | 4.05E-59 | -1.69E-46 |
| MS.gene056410 | MS.gene38351 | 0.833042 | 6.69E-56 | -1.69E-46 |
| MS.gene056451 | MS.gene38351 | 0.806722 | 7.17E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene38351 | 0.829717 | 4.40E-55 | -1.69E-46 |
| MS.gene056609 | MS.gene38351 | 0.844331 | 8.08E-59 | -1.69E-46 |
| MS.gene056612 | MS.gene38351 | 0.87102 | 9.39E-67 | -1.69E-46 |
| MS.gene056659 | MS.gene38351 | 0.821058 | 4.94E-53 | -1.69E-46 |
| MS.gene056796 | MS.gene38351 | 0.860783 | 1.62E-63 | -1.69E-46 |
| MS.gene056897 | MS.gene38351 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene38351 | -0.804083 | 2.56E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene38351 | 0.836979 | 6.81E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene38351 | 0.873845 | 1.07E-67 | -1.69E-46 |
| MS.gene057121 | MS.gene38351 | 0.810557 | 1.09E-50 | -1.69E-46 |
| MS.gene057123 | MS.gene38351 | 0.847841 | 8.96E-60 | -1.69E-46 |
| MS.gene057124 | MS.gene38351 | 0.832571 | 8.76E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene38351 | 0.828866 | 7.08E-55 | -1.69E-46 |
| MS.gene057146 | MS.gene38351 | 0.824592 | 7.42E-54 | -1.69E-46 |
| MS.gene057196 | MS.gene38351 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene057245 | MS.gene38351 | 0.802015 | 6.85E-49 | -1.69E-46 |
| MS.gene057295 | MS.gene38351 | 0.818222 | 2.19E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene38351 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene38351 | 0.855592 | 5.69E-62 | -1.69E-46 |
| MS.gene057416 | MS.gene38351 | 0.800669 | 1.29E-48 | -1.69E-46 |
| MS.gene057449 | MS.gene38351 | 0.800131 | 1.66E-48 | -1.69E-46 |
| MS.gene057450 | MS.gene38351 | 0.805626 | 1.22E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene38351 | 0.820735 | 5.86E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene38351 | 0.81912 | 1.37E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene38351 | 0.847027 | 1.50E-59 | -1.69E-46 |
| MS.gene058006 | MS.gene38351 | 0.830587 | 2.70E-55 | -1.69E-46 |
| MS.gene058082 | MS.gene38351 | 0.835846 | 1.32E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene38351 | 0.856153 | 3.90E-62 | -1.69E-46 |
| MS.gene058142 | MS.gene38351 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene38351 | 0.883283 | 5.12E-71 | -1.69E-46 |
| MS.gene058276 | MS.gene38351 | 0.839386 | 1.63E-57 | -1.69E-46 |
| MS.gene058293 | MS.gene38351 | 0.852973 | 3.25E-61 | -1.69E-46 |
| MS.gene058365 | MS.gene38351 | 0.826251 | 3.01E-54 | -1.69E-46 |
| MS.gene058376 | MS.gene38351 | 0.829649 | 4.58E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene38351 | 0.881462 | 2.35E-70 | -1.69E-46 |
| MS.gene058710 | MS.gene38351 | 0.815065 | 1.12E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene38351 | 0.807298 | 5.41E-50 | -1.69E-46 |
| MS.gene058787 | MS.gene38351 | 0.823307 | 1.49E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene38351 | 0.843724 | 1.18E-58 | -1.69E-46 |
| MS.gene05907 | MS.gene38351 | 0.869189 | 3.73E-66 | -1.69E-46 |
| MS.gene059141 | MS.gene38351 | 0.810767 | 9.79E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene38351 | 0.806592 | 7.63E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene38351 | 0.820459 | 6.78E-53 | -1.69E-46 |
| MS.gene059326 | MS.gene38351 | 0.847511 | 1.10E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene38351 | 0.806446 | 8.19E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene38351 | 0.81725 | 3.64E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene38351 | 0.825396 | 4.80E-54 | -1.69E-46 |
| MS.gene059536 | MS.gene38351 | 0.838593 | 2.62E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene38351 | 0.854077 | 1.56E-61 | -1.69E-46 |
| MS.gene05959 | MS.gene38351 | 0.818853 | 1.58E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene38351 | 0.860775 | 1.63E-63 | -1.69E-46 |
| MS.gene059688 | MS.gene38351 | 0.88103 | 3.37E-70 | -1.69E-46 |
| MS.gene059692 | MS.gene38351 | 0.895034 | 1.41E-75 | -1.69E-46 |
| MS.gene059695 | MS.gene38351 | 0.888792 | 4.31E-73 | -1.69E-46 |
| MS.gene059708 | MS.gene38351 | 0.816198 | 6.26E-52 | -1.69E-46 |
| MS.gene05984 | MS.gene38351 | 0.866523 | 2.68E-65 | -1.69E-46 |
| MS.gene059894 | MS.gene38351 | 0.894813 | 1.73E-75 | -1.69E-46 |
| MS.gene059902 | MS.gene38351 | 0.807219 | 5.63E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene38351 | 0.841435 | 4.76E-58 | -1.69E-46 |
| MS.gene06037 | MS.gene38351 | 0.851004 | 1.18E-60 | -1.69E-46 |
| MS.gene060403 | MS.gene38351 | 0.854106 | 1.54E-61 | -1.69E-46 |
| MS.gene060407 | MS.gene38351 | 0.835376 | 1.74E-56 | -1.69E-46 |
| MS.gene060804 | MS.gene38351 | 0.850223 | 1.95E-60 | -1.69E-46 |
| MS.gene060818 | MS.gene38351 | 0.828041 | 1.12E-54 | -1.69E-46 |
| MS.gene06090 | MS.gene38351 | 0.826314 | 2.90E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene38351 | 0.831643 | 1.49E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene38351 | 0.893623 | 5.29E-75 | -1.69E-46 |
| MS.gene061051 | MS.gene38351 | -0.829102 | 6.21E-55 | -1.69E-46 |
| MS.gene061154 | MS.gene38351 | 0.827404 | 1.59E-54 | -1.69E-46 |
| MS.gene061200 | MS.gene38351 | 0.80693 | 6.48E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38351.t1 | MTR_3g101740 | 91.975 | 162 | 7 | 1 | 1 | 156 | 57 | 218 | 8.39e-102 | 291 |
| MS.gene38351.t1 | MTR_3g112410 | 37.719 | 114 | 61 | 3 | 39 | 151 | 13 | 117 | 6.88e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38351.t1 | AT1G07700 | 76.515 | 132 | 30 | 1 | 20 | 151 | 74 | 204 | 5.83e-71 | 213 |
| MS.gene38351.t1 | AT1G07700 | 76.515 | 132 | 30 | 1 | 20 | 151 | 74 | 204 | 5.83e-71 | 213 |
| MS.gene38351.t1 | AT1G07700 | 76.515 | 132 | 30 | 1 | 20 | 151 | 74 | 204 | 5.83e-71 | 213 |
| MS.gene38351.t1 | AT1G07700 | 76.515 | 132 | 30 | 1 | 20 | 151 | 87 | 217 | 1.99e-70 | 211 |
| MS.gene38351.t1 | AT5G42980 | 33.628 | 113 | 67 | 2 | 39 | 151 | 12 | 116 | 2.69e-12 | 60.5 |
| MS.gene38351.t1 | AT3G51030 | 36.111 | 108 | 61 | 2 | 39 | 146 | 13 | 112 | 5.55e-12 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTCTTTGTCTCTGGTT+GGG | 0.298576 | 3.2:-84692478 | None:intergenic |
| AACAATCCTTTCTTTGTCTC+TGG | 0.330128 | 3.2:-84692483 | None:intergenic |
| CTAGATAAGTCTAAAGGAAC+TGG | 0.380813 | 3.2:+84691625 | MS.gene38351:CDS |
| GCAAAGTTGTGCAAGAAATC+TGG | 0.381106 | 3.2:+84691715 | MS.gene38351:CDS |
| TTGCGGAACGACTTAGAATC+AGG | 0.406477 | 3.2:+84692086 | MS.gene38351:CDS |
| AATTCTCTCACACATTCAAC+AGG | 0.416746 | 3.2:-84691574 | None:intergenic |
| TTATGCTTTAGAAAGATAAC+AGG | 0.421854 | 3.2:-84691751 | None:intergenic |
| ATCCTTTCTTTGTCTCTGGT+TGG | 0.431773 | 3.2:-84692479 | None:intergenic |
| TGCAACTTCCACAAGAGGTT+CGG | 0.443956 | 3.2:-84691672 | None:intergenic |
| CTCTTCCACTTCTACAAAGA+TGG | 0.449719 | 3.2:+84692437 | MS.gene38351:CDS |
| GATTTCTTCCGAACCTCTTG+TGG | 0.457067 | 3.2:+84691664 | MS.gene38351:CDS |
| AAGCCTTGTTGACATTCAAC+GGG | 0.478995 | 3.2:-84691523 | None:intergenic |
| ACATCATCATCATCTTCATC+AGG | 0.492703 | 3.2:-84691547 | None:intergenic |
| CAAGCCTTGTTGACATTCAA+CGG | 0.505128 | 3.2:-84691524 | None:intergenic |
| AAGTTGCAAGTATATTGAGC+AGG | 0.512637 | 3.2:+84691687 | MS.gene38351:CDS |
| CTACAAAGATGGAAAACTGT+TGG | 0.519244 | 3.2:+84692448 | MS.gene38351:CDS |
| AAAATTCTAGATAAGTCTAA+AGG | 0.519884 | 3.2:+84691619 | MS.gene38351:CDS |
| TCTAAGTCGTTCCGCAACCT+CGG | 0.520659 | 3.2:-84692080 | None:intergenic |
| AACTGGATCTCTTGTTGTGG+TGG | 0.529192 | 3.2:+84691642 | MS.gene38351:CDS |
| TGATGAAGAATCCGAGGTTG+CGG | 0.530168 | 3.2:+84692069 | MS.gene38351:CDS |
| TCCCAACCAGAGACAAAGAA+AGG | 0.565898 | 3.2:+84692477 | MS.gene38351:CDS |
| ATACTTGCAACTTCCACAAG+AGG | 0.567703 | 3.2:-84691677 | None:intergenic |
| TGGTACCTATTAATAAAATG+AGG | 0.575324 | 3.2:+84691488 | MS.gene38351:CDS |
| AGTTGCAAGTATATTGAGCA+GGG | 0.585364 | 3.2:+84691688 | MS.gene38351:CDS |
| CTTCAAGAGATGAGTACTTG+AGG | 0.592510 | 3.2:-84692514 | None:intergenic |
| AAGAGATGAGTACTTGAGGA+TGG | 0.618825 | 3.2:-84692510 | None:intergenic |
| TGCGGAACGACTTAGAATCA+GGG | 0.632999 | 3.2:+84692087 | MS.gene38351:CDS |
| TCTTTGTAGAAGTGGAAGAG+AGG | 0.655662 | 3.2:-84692434 | None:intergenic |
| AGGAACTGGATCTCTTGTTG+TGG | 0.658617 | 3.2:+84691639 | MS.gene38351:CDS |
| TATCCCGTTGAATGTCAACA+AGG | 0.662409 | 3.2:+84691520 | MS.gene38351:CDS |
| TGAGTATGATGAAGAATCCG+AGG | 0.729308 | 3.2:+84692063 | MS.gene38351:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTCAATAAGTTGATTT+TGG | - | chr3.2:84692390-84692409 | None:intergenic | 15.0% |
| !! | AAAATTCTAGATAAGTCTAA+AGG | + | chr3.2:84691619-84691638 | MS.gene38351:CDS | 20.0% |
| !! | ACTATTAGTTACAAATTAGT+TGG | + | chr3.2:84691825-84691844 | MS.gene38351:intron | 20.0% |
| !! | ATAATACAAATGTGATACAT+TGG | - | chr3.2:84691922-84691941 | None:intergenic | 20.0% |
| !! | TTAGATATTTATAGCATGTA+GGG | - | chr3.2:84692351-84692370 | None:intergenic | 20.0% |
| !!! | TTGTACCTCATTTTATTAAT+AGG | - | chr3.2:84691496-84691515 | None:intergenic | 20.0% |
| ! | GTTAGATATTTATAGCATGT+AGG | - | chr3.2:84692352-84692371 | None:intergenic | 25.0% |
| ! | TAATAATCACAAACTAATGC+AGG | + | chr3.2:84692406-84692425 | MS.gene38351:intron | 25.0% |
| ! | TGGTACCTATTAATAAAATG+AGG | + | chr3.2:84691488-84691507 | MS.gene38351:CDS | 25.0% |
| !! | TATGGATTAAAAACTGTTAG+CGG | + | chr3.2:84691853-84691872 | MS.gene38351:intron | 25.0% |
| !!! | TTATGCTTTAGAAAGATAAC+AGG | - | chr3.2:84691754-84691773 | None:intergenic | 25.0% |
| AAACATGAAGATGATACTGA+TGG | - | chr3.2:84692155-84692174 | None:intergenic | 30.0% | |
| ATCAATTACCTGCAATAAGA+GGG | - | chr3.2:84692044-84692063 | None:intergenic | 30.0% | |
| CTTAGTATGCATTCAATTGA+TGG | + | chr3.2:84692232-84692251 | MS.gene38351:intron | 30.0% | |
| TTAACTGTTCAGTTTGTCTA+TGG | + | chr3.2:84691796-84691815 | MS.gene38351:intron | 30.0% | |
| !! | ACAAATTAGTTGGTGACTTA+TGG | + | chr3.2:84691835-84691854 | MS.gene38351:intron | 30.0% |
| !! | GATGGATAATGTTGTTTCTT+AGG | + | chr3.2:84692250-84692269 | MS.gene38351:intron | 30.0% |
| AACAATCCTTTCTTTGTCTC+TGG | - | chr3.2:84692486-84692505 | None:intergenic | 35.0% | |
| AAGTTGCAAGTATATTGAGC+AGG | + | chr3.2:84691687-84691706 | MS.gene38351:CDS | 35.0% | |
| AATTCTCTCACACATTCAAC+AGG | - | chr3.2:84691577-84691596 | None:intergenic | 35.0% | |
| ACATCATCATCATCTTCATC+AGG | - | chr3.2:84691550-84691569 | None:intergenic | 35.0% | |
| AGTTGCAAGTATATTGAGCA+GGG | + | chr3.2:84691688-84691707 | MS.gene38351:CDS | 35.0% | |
| CATCAATTACCTGCAATAAG+AGG | - | chr3.2:84692045-84692064 | None:intergenic | 35.0% | |
| CTACAAAGATGGAAAACTGT+TGG | + | chr3.2:84692448-84692467 | MS.gene38351:CDS | 35.0% | |
| CTAGATAAGTCTAAAGGAAC+TGG | + | chr3.2:84691625-84691644 | MS.gene38351:CDS | 35.0% | |
| ! | GTTTTCCATCTTTGTAGAAG+TGG | - | chr3.2:84692445-84692464 | None:intergenic | 35.0% |
| AAGAGATGAGTACTTGAGGA+TGG | - | chr3.2:84692513-84692532 | None:intergenic | 40.0% | |
| ATACTTGCAACTTCCACAAG+AGG | - | chr3.2:84691680-84691699 | None:intergenic | 40.0% | |
| ATCCTTTCTTTGTCTCTGGT+TGG | - | chr3.2:84692482-84692501 | None:intergenic | 40.0% | |
| CATGAAGATGATACTGATGG+CGG | - | chr3.2:84692152-84692171 | None:intergenic | 40.0% | |
| CTCTTCCACTTCTACAAAGA+TGG | + | chr3.2:84692437-84692456 | MS.gene38351:CDS | 40.0% | |
| CTGTTCAGTTTGTCTATGGT+TGG | + | chr3.2:84691800-84691819 | MS.gene38351:intron | 40.0% | |
| CTTCAAGAGATGAGTACTTG+AGG | - | chr3.2:84692517-84692536 | None:intergenic | 40.0% | |
| GCAAAGTTGTGCAAGAAATC+TGG | + | chr3.2:84691715-84691734 | MS.gene38351:CDS | 40.0% | |
| TATCACTTCCCTCTTATTGC+AGG | + | chr3.2:84692033-84692052 | MS.gene38351:intron | 40.0% | |
| TATCCCGTTGAATGTCAACA+AGG | + | chr3.2:84691520-84691539 | MS.gene38351:CDS | 40.0% | |
| TCCTTTCTTTGTCTCTGGTT+GGG | - | chr3.2:84692481-84692500 | None:intergenic | 40.0% | |
| TCTTTGTAGAAGTGGAAGAG+AGG | - | chr3.2:84692437-84692456 | None:intergenic | 40.0% | |
| TGAGTATGATGAAGAATCCG+AGG | + | chr3.2:84692063-84692082 | MS.gene38351:CDS | 40.0% | |
| !! | AAGCCTTGTTGACATTCAAC+GGG | - | chr3.2:84691526-84691545 | None:intergenic | 40.0% |
| !! | CAAGCCTTGTTGACATTCAA+CGG | - | chr3.2:84691527-84691546 | None:intergenic | 40.0% |
| AGGAACTGGATCTCTTGTTG+TGG | + | chr3.2:84691639-84691658 | MS.gene38351:CDS | 45.0% | |
| GATTTCTTCCGAACCTCTTG+TGG | + | chr3.2:84691664-84691683 | MS.gene38351:CDS | 45.0% | |
| TCCCAACCAGAGACAAAGAA+AGG | + | chr3.2:84692477-84692496 | MS.gene38351:CDS | 45.0% | |
| TGATGAAGAATCCGAGGTTG+CGG | + | chr3.2:84692069-84692088 | MS.gene38351:CDS | 45.0% | |
| TGCAACTTCCACAAGAGGTT+CGG | - | chr3.2:84691675-84691694 | None:intergenic | 45.0% | |
| TGCGGAACGACTTAGAATCA+GGG | + | chr3.2:84692087-84692106 | MS.gene38351:CDS | 45.0% | |
| TTGCGGAACGACTTAGAATC+AGG | + | chr3.2:84692086-84692105 | MS.gene38351:CDS | 45.0% | |
| ! | AACTGGATCTCTTGTTGTGG+TGG | + | chr3.2:84691642-84691661 | MS.gene38351:CDS | 45.0% |
| TCTAAGTCGTTCCGCAACCT+CGG | - | chr3.2:84692083-84692102 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 84691487 | 84692556 | 84691487 | ID=MS.gene38351 |
| chr3.2 | mRNA | 84691487 | 84692556 | 84691487 | ID=MS.gene38351.t1;Parent=MS.gene38351 |
| chr3.2 | exon | 84691487 | 84691774 | 84691487 | ID=MS.gene38351.t1.exon1;Parent=MS.gene38351.t1 |
| chr3.2 | CDS | 84691487 | 84691774 | 84691487 | ID=cds.MS.gene38351.t1;Parent=MS.gene38351.t1 |
| chr3.2 | exon | 84692055 | 84692108 | 84692055 | ID=MS.gene38351.t1.exon2;Parent=MS.gene38351.t1 |
| chr3.2 | CDS | 84692055 | 84692108 | 84692055 | ID=cds.MS.gene38351.t1;Parent=MS.gene38351.t1 |
| chr3.2 | exon | 84692428 | 84692556 | 84692428 | ID=MS.gene38351.t1.exon3;Parent=MS.gene38351.t1 |
| chr3.2 | CDS | 84692428 | 84692556 | 84692428 | ID=cds.MS.gene38351.t1;Parent=MS.gene38351.t1 |
| Gene Sequence |
| Protein sequence |
