AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59353


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59353.t1 MTR_5g019920 96.825 126 4 0 1 126 43 168 1.86e-83 241
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59353.t1 AT3G50685 77.600 125 27 1 2 126 35 158 3.32e-63 190

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATCTCAATTCCTGTTAT+AGG 0.193029 5.4:+12107447 MS.gene59353:CDS
CTCCCTGCTGATCAGTGTTT+TGG 0.233472 5.4:+12107738 MS.gene59353:CDS
GGTCTTCCTAATGGTCCTTT+TGG 0.235150 5.4:+12107615 MS.gene59353:CDS
TGTTGAAGGGTTGTCATATT+TGG 0.326036 5.4:+12107650 MS.gene59353:CDS
TTTCAACAAGGTTACATTCC+TGG 0.368209 5.4:+12107711 MS.gene59353:CDS
GGGTTATTAGGTGCTGTTGA+AGG 0.373371 5.4:+12107636 MS.gene59353:CDS
GCTATTGCTGCTATCTCTGT+TGG 0.385488 5.4:-12107417 None:intergenic
TGCTGCTATCTCTGTTGGGC+TGG 0.397920 5.4:-12107412 None:intergenic
ACTGGTTCTGGTCTTCCTAA+TGG 0.397978 5.4:+12107606 MS.gene59353:CDS
CCAGGACCAGGTGGATCAAT+TGG 0.412152 5.4:+12107513 MS.gene59353:CDS
CTCTACACTCTCAAAACAAC+TGG 0.418846 5.4:+12107477 MS.gene59353:CDS
AATAACCCAAAAGGACCATT+AGG 0.423242 5.4:-12107621 None:intergenic
TAGAGAGACCAACCTATAAC+AGG 0.435409 5.4:-12107459 None:intergenic
GGATCAATTGGTGCATTAGA+AGG 0.445690 5.4:+12107525 MS.gene59353:CDS
TATCCAAAACACTGATCAGC+AGG 0.446026 5.4:-12107741 None:intergenic
CTATTGCTGCTATCTCTGTT+GGG 0.455946 5.4:-12107416 None:intergenic
TGTAAGTTATCTTGTAGTTG+TGG 0.468651 5.4:+12107548 MS.gene59353:CDS
GTAAGTTATCTTGTAGTTGT+GGG 0.479034 5.4:+12107549 MS.gene59353:CDS
TAGTTGTGGGAATTGTTGCT+TGG 0.495156 5.4:+12107562 MS.gene59353:CDS
CCAATTGATCCACCTGGTCC+TGG 0.509784 5.4:-12107513 None:intergenic
GGTTATTAGGTGCTGTTGAA+GGG 0.510819 5.4:+12107637 MS.gene59353:CDS
TGTGGTCTTCCACCAGGACC+AGG 0.516068 5.4:+12107501 MS.gene59353:CDS
AGGGTTGTCATATTTGGCAT+TGG 0.526933 5.4:+12107656 MS.gene59353:CDS
TCTCAATTCCTGTTATAGGT+TGG 0.527179 5.4:+12107451 MS.gene59353:CDS
TGATCAGCAGGGAGAGGACC+AGG 0.529852 5.4:-12107729 None:intergenic
ACAGAGATAGCAGCAATAGC+AGG 0.544713 5.4:+12107420 MS.gene59353:CDS
ACAGCACCTAATAACCCAAA+AGG 0.547610 5.4:-12107630 None:intergenic
GAGATAGCAGCAATAGCAGG+TGG 0.565377 5.4:+12107423 MS.gene59353:CDS
AAACACTGATCAGCAGGGAG+AGG 0.597184 5.4:-12107735 None:intergenic
ACTCTCAAAACAACTGGTTG+TGG 0.598063 5.4:+12107483 MS.gene59353:CDS
ATAACAAACATGGCAAAGGA+AGG 0.600615 5.4:+12107375 None:intergenic
ACTGGTTGTGGTCTTCCACC+AGG 0.608557 5.4:+12107495 MS.gene59353:CDS
CAAGATAACAAACATGGCAA+AGG 0.614035 5.4:+12107371 None:intergenic
ATTGATCCACCTGGTCCTGG+TGG 0.622088 5.4:-12107510 None:intergenic
GGTCTTCCACCAGGACCAGG+TGG 0.646487 5.4:+12107504 MS.gene59353:CDS
AATGCACCAATTGATCCACC+TGG 0.654079 5.4:-12107519 None:intergenic
AGATAGCAGCAATAGCAGGT+GGG 0.659143 5.4:+12107424 MS.gene59353:CDS
ATCCAAAACACTGATCAGCA+GGG 0.675935 5.4:-12107740 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATACCAAAAATAAAAC+TGG + chr5.4:12107588-12107607 MS.gene59353:CDS 15.0%
! TTAATCTCAATTCCTGTTAT+AGG + chr5.4:12107447-12107466 MS.gene59353:CDS 25.0%
GTAAGTTATCTTGTAGTTGT+GGG + chr5.4:12107549-12107568 MS.gene59353:CDS 30.0%
TGTAAGTTATCTTGTAGTTG+TGG + chr5.4:12107548-12107567 MS.gene59353:CDS 30.0%
! ACCAAAAATAAAACTGGTTC+TGG + chr5.4:12107594-12107613 MS.gene59353:CDS 30.0%
!!! ACCAGAACCAGTTTTATTTT+TGG - chr5.4:12107598-12107617 None:intergenic 30.0%
!!! GTTGCTATTGTTGTTGTTTT+TGG + chr5.4:12107678-12107697 MS.gene59353:CDS 30.0%
AATAACCCAAAAGGACCATT+AGG - chr5.4:12107624-12107643 None:intergenic 35.0%
TCTCAATTCCTGTTATAGGT+TGG + chr5.4:12107451-12107470 MS.gene59353:CDS 35.0%
TGTTGAAGGGTTGTCATATT+TGG + chr5.4:12107650-12107669 MS.gene59353:CDS 35.0%
TTTCAACAAGGTTACATTCC+TGG + chr5.4:12107711-12107730 MS.gene59353:CDS 35.0%
! GGTTTGCAGTATTTTCAACA+AGG + chr5.4:12107699-12107718 MS.gene59353:CDS 35.0%
!!! AATGGTCCTTTTGGGTTATT+AGG + chr5.4:12107624-12107643 MS.gene59353:CDS 35.0%
ACAGCACCTAATAACCCAAA+AGG - chr5.4:12107633-12107652 None:intergenic 40.0%
ACTCTCAAAACAACTGGTTG+TGG + chr5.4:12107483-12107502 MS.gene59353:CDS 40.0%
AGGGTTGTCATATTTGGCAT+TGG + chr5.4:12107656-12107675 MS.gene59353:CDS 40.0%
ATCCAAAACACTGATCAGCA+GGG - chr5.4:12107743-12107762 None:intergenic 40.0%
CTATTGCTGCTATCTCTGTT+GGG - chr5.4:12107419-12107438 None:intergenic 40.0%
CTCTACACTCTCAAAACAAC+TGG + chr5.4:12107477-12107496 MS.gene59353:CDS 40.0%
GGATCAATTGGTGCATTAGA+AGG + chr5.4:12107525-12107544 MS.gene59353:CDS 40.0%
TAGAGAGACCAACCTATAAC+AGG - chr5.4:12107462-12107481 None:intergenic 40.0%
TAGTTGTGGGAATTGTTGCT+TGG + chr5.4:12107562-12107581 MS.gene59353:CDS 40.0%
TATCCAAAACACTGATCAGC+AGG - chr5.4:12107744-12107763 None:intergenic 40.0%
! GTCTTCCTAATGGTCCTTTT+GGG + chr5.4:12107616-12107635 MS.gene59353:CDS 40.0%
!! GGTTATTAGGTGCTGTTGAA+GGG + chr5.4:12107637-12107656 MS.gene59353:CDS 40.0%
AATGCACCAATTGATCCACC+TGG - chr5.4:12107522-12107541 None:intergenic 45.0%
ACAGAGATAGCAGCAATAGC+AGG + chr5.4:12107420-12107439 MS.gene59353:CDS 45.0%
AGATAGCAGCAATAGCAGGT+GGG + chr5.4:12107424-12107443 MS.gene59353:CDS 45.0%
GCTATTGCTGCTATCTCTGT+TGG - chr5.4:12107420-12107439 None:intergenic 45.0%
! GGTCTTCCTAATGGTCCTTT+TGG + chr5.4:12107615-12107634 MS.gene59353:CDS 45.0%
!! ACTGGTTCTGGTCTTCCTAA+TGG + chr5.4:12107606-12107625 MS.gene59353:CDS 45.0%
!! GGGTTATTAGGTGCTGTTGA+AGG + chr5.4:12107636-12107655 MS.gene59353:CDS 45.0%
AAACACTGATCAGCAGGGAG+AGG - chr5.4:12107738-12107757 None:intergenic 50.0%
GAGATAGCAGCAATAGCAGG+TGG + chr5.4:12107423-12107442 MS.gene59353:CDS 50.0%
! CTCCCTGCTGATCAGTGTTT+TGG + chr5.4:12107738-12107757 MS.gene59353:CDS 50.0%
ACTGGTTGTGGTCTTCCACC+AGG + chr5.4:12107495-12107514 MS.gene59353:CDS 55.0%
ATTGATCCACCTGGTCCTGG+TGG - chr5.4:12107513-12107532 None:intergenic 55.0%
CCAATTGATCCACCTGGTCC+TGG - chr5.4:12107516-12107535 None:intergenic 55.0%
CCAGGACCAGGTGGATCAAT+TGG + chr5.4:12107513-12107532 MS.gene59353:CDS 55.0%
TGCTGCTATCTCTGTTGGGC+TGG - chr5.4:12107415-12107434 None:intergenic 55.0%
TGATCAGCAGGGAGAGGACC+AGG - chr5.4:12107732-12107751 None:intergenic 60.0%
TGTGGTCTTCCACCAGGACC+AGG + chr5.4:12107501-12107520 MS.gene59353:CDS 60.0%
GGTCTTCCACCAGGACCAGG+TGG + chr5.4:12107504-12107523 MS.gene59353:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 12107384 12107764 12107384 ID=MS.gene59353
chr5.4 mRNA 12107384 12107764 12107384 ID=MS.gene59353.t1;Parent=MS.gene59353
chr5.4 exon 12107384 12107764 12107384 ID=MS.gene59353.t1.exon1;Parent=MS.gene59353.t1
chr5.4 CDS 12107384 12107764 12107384 ID=cds.MS.gene59353.t1;Parent=MS.gene59353.t1
Gene Sequence

>MS.gene59353

ATGGCAAAGGAAGGAAGCGACACTAACACCAGCCCAACAGAGATAGCAGCAATAGCAGGTGGGTTAATCTCAATTCCTGTTATAGGTTGGTCTCTCTACACTCTCAAAACAACTGGTTGTGGTCTTCCACCAGGACCAGGTGGATCAATTGGTGCATTAGAAGGTGTAAGTTATCTTGTAGTTGTGGGAATTGTTGCTTGGTCTTTATATACCAAAAATAAAACTGGTTCTGGTCTTCCTAATGGTCCTTTTGGGTTATTAGGTGCTGTTGAAGGGTTGTCATATTTGGCATTGGTTGCTATTGTTGTTGTTTTTGGTTTGCAGTATTTTCAACAAGGTTACATTCCTGGTCCTCTCCCTGCTGATCAGTGTTTTGGATAG

Protein sequence

>MS.gene59353.t1

MAKEGSDTNTSPTEIAAIAGGLISIPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSLYTKNKTGSGLPNGPFGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG