Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76520.t1 | XP_003597708.1 | 86.8 | 273 | 28 | 1 | 1 | 265 | 1 | 273 | 1.80E-109 | 405.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76520.t1 | G7IUN5 | 86.8 | 273 | 28 | 1 | 1 | 265 | 1 | 273 | 1.3e-109 | 405.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene76520 | 0.802635 | 5.11E-49 | -1.69E-46 |
| MS.gene049173 | MS.gene76520 | 0.805194 | 1.50E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene76520 | 0.841202 | 5.48E-58 | -1.69E-46 |
| MS.gene049222 | MS.gene76520 | 0.808292 | 3.33E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene76520 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene76520 | 0.819475 | 1.14E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene76520 | 0.809847 | 1.55E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene76520 | 0.800738 | 1.25E-48 | -1.69E-46 |
| MS.gene049369 | MS.gene76520 | 0.821228 | 4.51E-53 | -1.69E-46 |
| MS.gene049427 | MS.gene76520 | 0.819464 | 1.15E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene76520 | 0.82957 | 4.78E-55 | -1.69E-46 |
| MS.gene049661 | MS.gene76520 | 0.810966 | 8.86E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene76520 | 0.825903 | 3.64E-54 | -1.69E-46 |
| MS.gene049907 | MS.gene76520 | 0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene050163 | MS.gene76520 | 0.840867 | 6.71E-58 | -1.69E-46 |
| MS.gene050165 | MS.gene76520 | 0.827624 | 1.41E-54 | -1.69E-46 |
| MS.gene050333 | MS.gene76520 | 0.819313 | 1.24E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene76520 | 0.844176 | 8.89E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene76520 | 0.800833 | 1.20E-48 | -1.69E-46 |
| MS.gene050518 | MS.gene76520 | 0.813043 | 3.13E-51 | -1.69E-46 |
| MS.gene050520 | MS.gene76520 | 0.822939 | 1.81E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene76520 | 0.83266 | 8.33E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene76520 | 0.821546 | 3.81E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene76520 | 0.806215 | 9.16E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene76520 | 0.843383 | 1.45E-58 | -1.69E-46 |
| MS.gene050686 | MS.gene76520 | 0.801787 | 7.64E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene76520 | 0.821077 | 4.89E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene76520 | 0.850607 | 1.52E-60 | -1.69E-46 |
| MS.gene050770 | MS.gene76520 | 0.850585 | 1.54E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene76520 | 0.845052 | 5.17E-59 | -1.69E-46 |
| MS.gene05080 | MS.gene76520 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene76520 | 0.817899 | 2.59E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene76520 | 0.800852 | 1.19E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene76520 | 0.840035 | 1.11E-57 | -1.69E-46 |
| MS.gene051228 | MS.gene76520 | 0.845862 | 3.12E-59 | -1.69E-46 |
| MS.gene051434 | MS.gene76520 | 0.802851 | 4.61E-49 | -1.69E-46 |
| MS.gene051617 | MS.gene76520 | 0.813666 | 2.28E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene76520 | 0.824505 | 7.78E-54 | -1.69E-46 |
| MS.gene051692 | MS.gene76520 | 0.813899 | 2.03E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene76520 | 0.863603 | 2.21E-64 | -1.69E-46 |
| MS.gene051858 | MS.gene76520 | 0.830678 | 2.57E-55 | -1.69E-46 |
| MS.gene052078 | MS.gene76520 | 0.818969 | 1.48E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene76520 | 0.816435 | 5.54E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene76520 | 0.843191 | 1.63E-58 | -1.69E-46 |
| MS.gene052310 | MS.gene76520 | 0.813652 | 2.30E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene76520 | 0.823287 | 1.50E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene76520 | 0.809205 | 2.12E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene76520 | 0.831712 | 1.43E-55 | -1.69E-46 |
| MS.gene052485 | MS.gene76520 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene052508 | MS.gene76520 | 0.803827 | 2.89E-49 | -1.69E-46 |
| MS.gene052510 | MS.gene76520 | 0.803494 | 3.39E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene76520 | 0.823998 | 1.02E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene76520 | 0.841498 | 4.58E-58 | -1.69E-46 |
| MS.gene053207 | MS.gene76520 | 0.866471 | 2.78E-65 | -1.69E-46 |
| MS.gene053209 | MS.gene76520 | 0.833584 | 4.90E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene76520 | 0.826381 | 2.80E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene76520 | 0.814842 | 1.25E-51 | -1.69E-46 |
| MS.gene053322 | MS.gene76520 | 0.82077 | 5.76E-53 | -1.69E-46 |
| MS.gene053574 | MS.gene76520 | 0.809687 | 1.67E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene76520 | 0.848793 | 4.89E-60 | -1.69E-46 |
| MS.gene053813 | MS.gene76520 | 0.824981 | 6.01E-54 | -1.69E-46 |
| MS.gene053915 | MS.gene76520 | 0.803839 | 2.88E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene76520 | 0.831024 | 2.11E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene76520 | 0.849939 | 2.34E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene76520 | 0.835237 | 1.89E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene76520 | 0.836408 | 9.52E-57 | -1.69E-46 |
| MS.gene054802 | MS.gene76520 | 0.817336 | 3.48E-52 | -1.69E-46 |
| MS.gene054803 | MS.gene76520 | 0.818515 | 1.88E-52 | -1.69E-46 |
| MS.gene054804 | MS.gene76520 | 0.823436 | 1.39E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene76520 | 0.82519 | 5.37E-54 | -1.69E-46 |
| MS.gene055206 | MS.gene76520 | 0.831583 | 1.54E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene76520 | 0.83176 | 1.39E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene76520 | 0.809836 | 1.55E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene76520 | 0.868353 | 6.96E-66 | -1.69E-46 |
| MS.gene055499 | MS.gene76520 | 0.858165 | 9.93E-63 | -1.69E-46 |
| MS.gene055502 | MS.gene76520 | 0.803315 | 3.70E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene76520 | 0.824717 | 6.94E-54 | -1.69E-46 |
| MS.gene055636 | MS.gene76520 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene055638 | MS.gene76520 | 0.81201 | 5.26E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene76520 | 0.843327 | 1.50E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene76520 | 0.841908 | 3.57E-58 | -1.69E-46 |
| MS.gene05592 | MS.gene76520 | 0.868163 | 8.01E-66 | -1.69E-46 |
| MS.gene055941 | MS.gene76520 | 0.808563 | 2.91E-50 | -1.69E-46 |
| MS.gene05602 | MS.gene76520 | 0.837226 | 5.89E-57 | -1.69E-46 |
| MS.gene056083 | MS.gene76520 | 0.800716 | 1.26E-48 | -1.69E-46 |
| MS.gene056374 | MS.gene76520 | 0.822419 | 2.39E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene76520 | 0.808439 | 3.10E-50 | -1.69E-46 |
| MS.gene056449 | MS.gene76520 | 0.800572 | 1.35E-48 | -1.69E-46 |
| MS.gene056487 | MS.gene76520 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene056609 | MS.gene76520 | 0.856142 | 3.93E-62 | -1.69E-46 |
| MS.gene056657 | MS.gene76520 | 0.818662 | 1.74E-52 | -1.69E-46 |
| MS.gene05666 | MS.gene76520 | 0.800856 | 1.18E-48 | -1.69E-46 |
| MS.gene056897 | MS.gene76520 | 0.812213 | 4.75E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene76520 | 0.832722 | 8.04E-56 | -1.69E-46 |
| MS.gene057124 | MS.gene76520 | 0.8446 | 6.84E-59 | -1.69E-46 |
| MS.gene057129 | MS.gene76520 | 0.884467 | 1.87E-71 | -1.69E-46 |
| MS.gene057146 | MS.gene76520 | 0.801932 | 7.13E-49 | -1.69E-46 |
| MS.gene057295 | MS.gene76520 | 0.809831 | 1.56E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene76520 | 0.846353 | 2.29E-59 | -1.69E-46 |
| MS.gene05735 | MS.gene76520 | 0.838175 | 3.36E-57 | -1.69E-46 |
| MS.gene057416 | MS.gene76520 | 0.839604 | 1.43E-57 | -1.69E-46 |
| MS.gene057734 | MS.gene76520 | 0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene76520 | 0.830842 | 2.34E-55 | -1.69E-46 |
| MS.gene058006 | MS.gene76520 | 0.813167 | 2.93E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene76520 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene058082 | MS.gene76520 | 0.814168 | 1.77E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene76520 | 0.835521 | 1.60E-56 | -1.69E-46 |
| MS.gene058323 | MS.gene76520 | 0.821403 | 4.11E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene76520 | 0.838238 | 3.24E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene76520 | 0.829447 | 5.12E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene76520 | 0.825478 | 4.59E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene76520 | 0.855432 | 6.33E-62 | -1.69E-46 |
| MS.gene058748 | MS.gene76520 | 0.822827 | 1.92E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene76520 | 0.810334 | 1.21E-50 | -1.69E-46 |
| MS.gene058787 | MS.gene76520 | 0.810316 | 1.22E-50 | -1.69E-46 |
| MS.gene058993 | MS.gene76520 | 0.852189 | 5.43E-61 | -1.69E-46 |
| MS.gene059029 | MS.gene76520 | 0.82866 | 7.94E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene76520 | 0.808984 | 2.37E-50 | -1.69E-46 |
| MS.gene059536 | MS.gene76520 | 0.800773 | 1.23E-48 | -1.69E-46 |
| MS.gene059676 | MS.gene76520 | 0.822138 | 2.78E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene76520 | 0.826105 | 3.26E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene76520 | 0.81777 | 2.77E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene76520 | 0.82816 | 1.05E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene76520 | 0.829151 | 6.04E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene76520 | 0.807491 | 4.93E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene76520 | 0.834293 | 3.26E-56 | -1.69E-46 |
| MS.gene05986 | MS.gene76520 | 0.806951 | 6.41E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene76520 | 0.822328 | 2.51E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene76520 | 0.848876 | 4.63E-60 | -1.69E-46 |
| MS.gene060193 | MS.gene76520 | 0.824517 | 7.73E-54 | -1.69E-46 |
| MS.gene060356 | MS.gene76520 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene76520 | 0.831685 | 1.45E-55 | -1.69E-46 |
| MS.gene060818 | MS.gene76520 | 0.83311 | 6.44E-56 | -1.69E-46 |
| MS.gene060835 | MS.gene76520 | 0.846068 | 2.74E-59 | -1.69E-46 |
| MS.gene060837 | MS.gene76520 | 0.809491 | 1.84E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene76520 | 0.808275 | 3.36E-50 | -1.69E-46 |
| MS.gene061041 | MS.gene76520 | 0.819742 | 9.90E-53 | -1.69E-46 |
| MS.gene061051 | MS.gene76520 | -0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene76520 | 0.809326 | 2.00E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene76520 | 0.853684 | 2.03E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76520.t1 | MTR_2g101430 | 90.110 | 273 | 19 | 1 | 1 | 265 | 1 | 273 | 3.09e-179 | 494 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76520.t1 | AT1G51080 | 48.416 | 221 | 103 | 4 | 47 | 259 | 63 | 280 | 1.31e-55 | 180 |
Find 75 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACCTTCTCGGCACTTCTA+AGG | 0.214375 | 2.3:-2645574 | MS.gene76520:CDS |
| TTTGCTCTTAATGTGTTAAA+TGG | 0.235237 | 2.3:-2645717 | MS.gene76520:CDS |
| TCATGTTTACTTTATGTTTC+GGG | 0.243260 | 2.3:-2646006 | MS.gene76520:CDS |
| GTTAGGTTGGGTGTTGGTAT+TGG | 0.289295 | 2.3:+2645509 | None:intergenic |
| ATCATGTTTACTTTATGTTT+CGG | 0.295381 | 2.3:-2646007 | MS.gene76520:CDS |
| TAGCAGGTGGGTGGTTAGGT+TGG | 0.367914 | 2.3:+2645496 | None:intergenic |
| AAAGTTGATTTCGACCTTCT+CGG | 0.376013 | 2.3:-2645585 | MS.gene76520:CDS |
| TAGGATGCTGCCATGGTTGA+TGG | 0.394720 | 2.3:+2645263 | None:intergenic |
| AGAGACAGAATAATAGATTT+CGG | 0.398637 | 2.3:-2645891 | MS.gene76520:CDS |
| TCAATGCGGTCTTTGTAGAT+TGG | 0.399152 | 2.3:+2645747 | None:intergenic |
| GAAATCTATTATTCTGTCTC+TGG | 0.401072 | 2.3:+2645893 | None:intergenic |
| CTTAAGAGCACGCGAGTTTG+AGG | 0.411946 | 2.3:-2645805 | MS.gene76520:CDS |
| CTAGTAGCAGGTGGGTGGTT+AGG | 0.415512 | 2.3:+2645492 | None:intergenic |
| CAATCCATTCCCCGGACGTC+AGG | 0.418797 | 2.3:-2645301 | MS.gene76520:CDS |
| TCTGCTGCTGCTAGATCCAT+TGG | 0.419249 | 2.3:-2645681 | MS.gene76520:CDS |
| CTTTGTAGATTGGGTCTAGA+AGG | 0.419945 | 2.3:+2645757 | None:intergenic |
| CAATGCGGTCTTTGTAGATT+GGG | 0.420162 | 2.3:+2645748 | None:intergenic |
| TTTAAAGTTGAGTTTAGCTT+AGG | 0.423436 | 2.3:+2645244 | None:intergenic |
| AGCAGGTGGGTGGTTAGGTT+GGG | 0.433268 | 2.3:+2645497 | None:intergenic |
| CAGGAGATAAGCAAGAGATT+TGG | 0.434593 | 2.3:-2645639 | MS.gene76520:CDS |
| TTCAGAAAGAGAAATTAAAC+TGG | 0.440028 | 2.3:-2645916 | MS.gene76520:CDS |
| TGGGTGGTTAGGTTGGGTGT+TGG | 0.441793 | 2.3:+2645503 | None:intergenic |
| CTCAGAATCTGCCGCTCCAA+TGG | 0.465779 | 2.3:+2645665 | None:intergenic |
| ACGGAGAAGAGCCTGACGTC+CGG | 0.467710 | 2.3:+2645290 | None:intergenic |
| AAATCAAAAGCAGCGTTATG+TGG | 0.472830 | 2.3:-2645967 | MS.gene76520:CDS |
| GATAAGCAAGAGATTTGGTT+GGG | 0.481364 | 2.3:-2645634 | MS.gene76520:CDS |
| AGAGCCTGACGTCCGGGGAA+TGG | 0.482518 | 2.3:+2645297 | None:intergenic |
| AAATACAAGGGGAAGATGTT+GGG | 0.485271 | 2.3:-2645867 | MS.gene76520:CDS |
| CGGAGAAGAGCCTGACGTCC+GGG | 0.490784 | 2.3:+2645291 | None:intergenic |
| GACCTTCTCGGCACTTCTAA+GGG | 0.491235 | 2.3:-2645573 | MS.gene76520:CDS |
| AAAATACAAGGGGAAGATGT+TGG | 0.491381 | 2.3:-2645868 | MS.gene76520:CDS |
| CTGACGTCCGGGGAATGGAT+TGG | 0.498352 | 2.3:+2645302 | None:intergenic |
| AGCGGCAGATTCTGAGCTGC+AGG | 0.508668 | 2.3:-2645658 | MS.gene76520:CDS |
| CCACATTGTTGTCCTTCTGT+TGG | 0.509399 | 2.3:+2645370 | None:intergenic |
| TGCCATGGTTGATGGCCTTA+CGG | 0.514572 | 2.3:+2645271 | None:intergenic |
| TCGCTGAAATTGACAAGTCT+CGG | 0.520757 | 2.3:+2645540 | None:intergenic |
| TTCCGTCGAGCTACCTCAAG+TGG | 0.521226 | 2.3:-2645839 | MS.gene76520:CDS |
| AGCACGCGAGTTTGAGGAGT+GGG | 0.522721 | 2.3:-2645799 | MS.gene76520:CDS |
| ACAGTAGTAGTAGGACTAAG+AGG | 0.528829 | 2.3:-2645446 | MS.gene76520:CDS |
| AATAGATTTCGGAAAATACA+AGG | 0.531136 | 2.3:-2645880 | MS.gene76520:CDS |
| GCCATTGCCAATCCATTCCC+CGG | 0.538546 | 2.3:-2645309 | MS.gene76520:CDS |
| ACGGTAGTAACAGTAGTAGT+AGG | 0.539912 | 2.3:-2645455 | MS.gene76520:CDS |
| CTCCCTTAGAAGTGCCGAGA+AGG | 0.555291 | 2.3:+2645571 | None:intergenic |
| AATCAAAAGCAGCGTTATGT+GGG | 0.565427 | 2.3:-2645966 | MS.gene76520:CDS |
| TGCTCCAACCAACTTTGTCA+AGG | 0.565623 | 2.3:+2645607 | None:intergenic |
| ACGTCAGGCTCTTCTCCGTA+AGG | 0.566162 | 2.3:-2645286 | MS.gene76520:CDS |
| AAATGAAGCGCACCAACAGA+AGG | 0.567882 | 2.3:-2645382 | MS.gene76520:CDS |
| AGATAAGCAAGAGATTTGGT+TGG | 0.569741 | 2.3:-2645635 | MS.gene76520:CDS |
| TAGTAGTAGGACTAAGAGGA+TGG | 0.572795 | 2.3:-2645442 | MS.gene76520:CDS |
| TCCGGGGAATGGATTGGCAA+TGG | 0.584712 | 2.3:+2645308 | None:intergenic |
| ACTAGTATCGTTGGTAGCAA+CGG | 0.585566 | 2.3:-2645474 | MS.gene76520:CDS |
| TGGGATAACCTTGACAAAGT+TGG | 0.586673 | 2.3:-2645615 | MS.gene76520:CDS |
| ATAACCTTGACAAAGTTGGT+TGG | 0.591237 | 2.3:-2645611 | MS.gene76520:CDS |
| TTCTCGGCACTTCTAAGGGA+GGG | 0.601983 | 2.3:-2645569 | MS.gene76520:CDS |
| TTTAGCTTAGGATGCTGCCA+TGG | 0.602732 | 2.3:+2645256 | None:intergenic |
| CTACCAACGATACTAGTAGC+AGG | 0.604969 | 2.3:+2645480 | None:intergenic |
| CTCCGTAAGGCCATCAACCA+TGG | 0.607049 | 2.3:-2645273 | MS.gene76520:CDS |
| TCTACAAAGACCGCATTGAG+TGG | 0.608441 | 2.3:-2645743 | MS.gene76520:CDS |
| TCCGTCGAGCTACCTCAAGT+GGG | 0.610493 | 2.3:-2645838 | MS.gene76520:CDS |
| ATAGATTTCGGAAAATACAA+GGG | 0.628131 | 2.3:-2645879 | MS.gene76520:CDS |
| CCACCTGCTACTAGTATCGT+TGG | 0.631045 | 2.3:-2645483 | MS.gene76520:CDS |
| CGAGACAGAAAATGAGATCG+AGG | 0.635307 | 2.3:-2645410 | MS.gene76520:CDS |
| CTACAAAGACCGCATTGAGT+GGG | 0.639133 | 2.3:-2645742 | MS.gene76520:CDS |
| GGAGAAGAGCCTGACGTCCG+GGG | 0.642345 | 2.3:+2645292 | None:intergenic |
| CGATACTAGTAGCAGGTGGG+TGG | 0.643375 | 2.3:+2645487 | None:intergenic |
| CTTCTCGGCACTTCTAAGGG+AGG | 0.649267 | 2.3:-2645570 | MS.gene76520:CDS |
| TAGATTTCGGAAAATACAAG+GGG | 0.656423 | 2.3:-2645878 | MS.gene76520:CDS |
| CCAACGATACTAGTAGCAGG+TGG | 0.663999 | 2.3:+2645483 | None:intergenic |
| ACTTGAGGTAGCTCGACGGA+AGG | 0.664751 | 2.3:+2645841 | None:intergenic |
| TGCTGCTAGATCCATTGGAG+CGG | 0.676565 | 2.3:-2645676 | MS.gene76520:CDS |
| ACCCACTTGAGGTAGCTCGA+CGG | 0.680643 | 2.3:+2645837 | None:intergenic |
| CAACGATACTAGTAGCAGGT+GGG | 0.693048 | 2.3:+2645484 | None:intergenic |
| CCAACAGAAGGACAACAATG+TGG | 0.706083 | 2.3:-2645370 | MS.gene76520:CDS |
| GAGCAAACTCCCACTCAATG+CGG | 0.708509 | 2.3:+2645733 | None:intergenic |
| GAGCACGCGAGTTTGAGGAG+TGG | 0.717265 | 2.3:-2645800 | MS.gene76520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGTTTACTTTATGTTT+CGG | - | chr2.3:2645282-2645301 | MS.gene76520:CDS | 20.0% |
| ! | AATAGATTTCGGAAAATACA+AGG | - | chr2.3:2645409-2645428 | MS.gene76520:CDS | 25.0% |
| ! | AGAGACAGAATAATAGATTT+CGG | - | chr2.3:2645398-2645417 | MS.gene76520:CDS | 25.0% |
| ! | ATAGATTTCGGAAAATACAA+GGG | - | chr2.3:2645410-2645429 | MS.gene76520:CDS | 25.0% |
| ! | TCATGTTTACTTTATGTTTC+GGG | - | chr2.3:2645283-2645302 | MS.gene76520:CDS | 25.0% |
| ! | TTCAGAAAGAGAAATTAAAC+TGG | - | chr2.3:2645373-2645392 | MS.gene76520:CDS | 25.0% |
| ! | TTTGCTCTTAATGTGTTAAA+TGG | - | chr2.3:2645572-2645591 | MS.gene76520:CDS | 25.0% |
| GAAATCTATTATTCTGTCTC+TGG | + | chr2.3:2645399-2645418 | None:intergenic | 30.0% | |
| TAGATTTCGGAAAATACAAG+GGG | - | chr2.3:2645411-2645430 | MS.gene76520:CDS | 30.0% | |
| AAAATACAAGGGGAAGATGT+TGG | - | chr2.3:2645421-2645440 | MS.gene76520:CDS | 35.0% | |
| AAATACAAGGGGAAGATGTT+GGG | - | chr2.3:2645422-2645441 | MS.gene76520:CDS | 35.0% | |
| AAATCAAAAGCAGCGTTATG+TGG | - | chr2.3:2645322-2645341 | MS.gene76520:CDS | 35.0% | |
| AATCAAAAGCAGCGTTATGT+GGG | - | chr2.3:2645323-2645342 | MS.gene76520:CDS | 35.0% | |
| ! | AAAGTTGATTTCGACCTTCT+CGG | - | chr2.3:2645704-2645723 | MS.gene76520:CDS | 35.0% |
| ! | AGATAAGCAAGAGATTTGGT+TGG | - | chr2.3:2645654-2645673 | MS.gene76520:CDS | 35.0% |
| ! | ATAACCTTGACAAAGTTGGT+TGG | - | chr2.3:2645678-2645697 | MS.gene76520:CDS | 35.0% |
| ! | GATAAGCAAGAGATTTGGTT+GGG | - | chr2.3:2645655-2645674 | MS.gene76520:CDS | 35.0% |
| !!! | AGTTTTTTGAAACCCACTTG+AGG | + | chr2.3:2645466-2645485 | None:intergenic | 35.0% |
| ACAGTAGTAGTAGGACTAAG+AGG | - | chr2.3:2645843-2645862 | MS.gene76520:CDS | 40.0% | |
| ACGGTAGTAACAGTAGTAGT+AGG | - | chr2.3:2645834-2645853 | MS.gene76520:CDS | 40.0% | |
| CAATGCGGTCTTTGTAGATT+GGG | + | chr2.3:2645544-2645563 | None:intergenic | 40.0% | |
| CAGGAGATAAGCAAGAGATT+TGG | - | chr2.3:2645650-2645669 | MS.gene76520:CDS | 40.0% | |
| CTTTGTAGATTGGGTCTAGA+AGG | + | chr2.3:2645535-2645554 | None:intergenic | 40.0% | |
| TAGTAGTAGGACTAAGAGGA+TGG | - | chr2.3:2645847-2645866 | MS.gene76520:CDS | 40.0% | |
| TCAATGCGGTCTTTGTAGAT+TGG | + | chr2.3:2645545-2645564 | None:intergenic | 40.0% | |
| TCGCTGAAATTGACAAGTCT+CGG | + | chr2.3:2645752-2645771 | None:intergenic | 40.0% | |
| TGGGATAACCTTGACAAAGT+TGG | - | chr2.3:2645674-2645693 | MS.gene76520:CDS | 40.0% | |
| !! | ACTAGTATCGTTGGTAGCAA+CGG | - | chr2.3:2645815-2645834 | MS.gene76520:CDS | 40.0% |
| AAATGAAGCGCACCAACAGA+AGG | - | chr2.3:2645907-2645926 | MS.gene76520:CDS | 45.0% | |
| CAACGATACTAGTAGCAGGT+GGG | + | chr2.3:2645808-2645827 | None:intergenic | 45.0% | |
| CCAACAGAAGGACAACAATG+TGG | - | chr2.3:2645919-2645938 | MS.gene76520:CDS | 45.0% | |
| CCACATTGTTGTCCTTCTGT+TGG | + | chr2.3:2645922-2645941 | None:intergenic | 45.0% | |
| CGAGACAGAAAATGAGATCG+AGG | - | chr2.3:2645879-2645898 | MS.gene76520:CDS | 45.0% | |
| CTACAAAGACCGCATTGAGT+GGG | - | chr2.3:2645547-2645566 | MS.gene76520:CDS | 45.0% | |
| CTACCAACGATACTAGTAGC+AGG | + | chr2.3:2645812-2645831 | None:intergenic | 45.0% | |
| TCTACAAAGACCGCATTGAG+TGG | - | chr2.3:2645546-2645565 | MS.gene76520:CDS | 45.0% | |
| TGCTCCAACCAACTTTGTCA+AGG | + | chr2.3:2645685-2645704 | None:intergenic | 45.0% | |
| !! | GTTAGGTTGGGTGTTGGTAT+TGG | + | chr2.3:2645783-2645802 | None:intergenic | 45.0% |
| CCAACGATACTAGTAGCAGG+TGG | + | chr2.3:2645809-2645828 | None:intergenic | 50.0% | |
| CCACCTGCTACTAGTATCGT+TGG | - | chr2.3:2645806-2645825 | MS.gene76520:CDS | 50.0% | |
| GACCTTCTCGGCACTTCTAA+GGG | - | chr2.3:2645716-2645735 | MS.gene76520:CDS | 50.0% | |
| GAGCAAACTCCCACTCAATG+CGG | + | chr2.3:2645559-2645578 | None:intergenic | 50.0% | |
| TAGGATGCTGCCATGGTTGA+TGG | + | chr2.3:2646029-2646048 | None:intergenic | 50.0% | |
| TCTGCTGCTGCTAGATCCAT+TGG | - | chr2.3:2645608-2645627 | MS.gene76520:CDS | 50.0% | |
| TGCTGCTAGATCCATTGGAG+CGG | - | chr2.3:2645613-2645632 | MS.gene76520:CDS | 50.0% | |
| ! | CTTAAGAGCACGCGAGTTTG+AGG | - | chr2.3:2645484-2645503 | MS.gene76520:CDS | 50.0% |
| ! | TTCTCGGCACTTCTAAGGGA+GGG | - | chr2.3:2645720-2645739 | MS.gene76520:CDS | 50.0% |
| !! | TGCCATGGTTGATGGCCTTA+CGG | + | chr2.3:2646021-2646040 | None:intergenic | 50.0% |
| ACCCACTTGAGGTAGCTCGA+CGG | + | chr2.3:2645455-2645474 | None:intergenic | 55.0% | |
| ACGTCAGGCTCTTCTCCGTA+AGG | - | chr2.3:2646003-2646022 | MS.gene76520:CDS | 55.0% | |
| ACTTGAGGTAGCTCGACGGA+AGG | + | chr2.3:2645451-2645470 | None:intergenic | 55.0% | |
| AGCACGCGAGTTTGAGGAGT+GGG | - | chr2.3:2645490-2645509 | MS.gene76520:CDS | 55.0% | |
| AGCAGGTGGGTGGTTAGGTT+GGG | + | chr2.3:2645795-2645814 | None:intergenic | 55.0% | |
| CGACCTTCTCGGCACTTCTA+AGG | - | chr2.3:2645715-2645734 | MS.gene76520:CDS | 55.0% | |
| CGATACTAGTAGCAGGTGGG+TGG | + | chr2.3:2645805-2645824 | None:intergenic | 55.0% | |
| CTAGTAGCAGGTGGGTGGTT+AGG | + | chr2.3:2645800-2645819 | None:intergenic | 55.0% | |
| CTCAGAATCTGCCGCTCCAA+TGG | + | chr2.3:2645627-2645646 | None:intergenic | 55.0% | |
| CTCCGTAAGGCCATCAACCA+TGG | - | chr2.3:2646016-2646035 | MS.gene76520:CDS | 55.0% | |
| GCCATTGCCAATCCATTCCC+CGG | - | chr2.3:2645980-2645999 | MS.gene76520:CDS | 55.0% | |
| TAGCAGGTGGGTGGTTAGGT+TGG | + | chr2.3:2645796-2645815 | None:intergenic | 55.0% | |
| TCCGTCGAGCTACCTCAAGT+GGG | - | chr2.3:2645451-2645470 | MS.gene76520:CDS | 55.0% | |
| TTCCGTCGAGCTACCTCAAG+TGG | - | chr2.3:2645450-2645469 | MS.gene76520:CDS | 55.0% | |
| ! | CTTCTCGGCACTTCTAAGGG+AGG | - | chr2.3:2645719-2645738 | MS.gene76520:CDS | 55.0% |
| !! | CTCCCTTAGAAGTGCCGAGA+AGG | + | chr2.3:2645721-2645740 | None:intergenic | 55.0% |
| !! | TCCGGGGAATGGATTGGCAA+TGG | + | chr2.3:2645984-2646003 | None:intergenic | 55.0% |
| !! | TGGGTGGTTAGGTTGGGTGT+TGG | + | chr2.3:2645789-2645808 | None:intergenic | 55.0% |
| ACGGAGAAGAGCCTGACGTC+CGG | + | chr2.3:2646002-2646021 | None:intergenic | 60.0% | |
| AGCGGCAGATTCTGAGCTGC+AGG | - | chr2.3:2645631-2645650 | MS.gene76520:CDS | 60.0% | |
| CAATCCATTCCCCGGACGTC+AGG | - | chr2.3:2645988-2646007 | MS.gene76520:CDS | 60.0% | |
| CTGACGTCCGGGGAATGGAT+TGG | + | chr2.3:2645990-2646009 | None:intergenic | 60.0% | |
| GAGCACGCGAGTTTGAGGAG+TGG | - | chr2.3:2645489-2645508 | MS.gene76520:CDS | 60.0% | |
| AGAGCCTGACGTCCGGGGAA+TGG | + | chr2.3:2645995-2646014 | None:intergenic | 65.0% | |
| CGGAGAAGAGCCTGACGTCC+GGG | + | chr2.3:2646001-2646020 | None:intergenic | 65.0% | |
| GGAGAAGAGCCTGACGTCCG+GGG | + | chr2.3:2646000-2646019 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 2645257 | 2646054 | 2645257 | ID=MS.gene76520 |
| chr2.3 | mRNA | 2645257 | 2646054 | 2645257 | ID=MS.gene76520.t1;Parent=MS.gene76520 |
| chr2.3 | exon | 2645257 | 2646054 | 2645257 | ID=MS.gene76520.t1.exon1;Parent=MS.gene76520.t1 |
| chr2.3 | CDS | 2645257 | 2646054 | 2645257 | ID=cds.MS.gene76520.t1;Parent=MS.gene76520.t1 |
| Gene Sequence |
| Protein sequence |
