AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0074230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074230 MtrunA17_Chr1g0181901 90.842 404 8 2 1 375 1 404 0.0 735
Msa0074230 MtrunA17_Chr7g0263281 77.540 374 79 1 1 374 1 369 0.0 591
Msa0074230 MtrunA17_Chr1g0213351 62.634 372 128 3 1 371 9 370 6.65e-170 479
Msa0074230 MtrunA17_Chr7g0250061 66.197 355 119 1 18 371 18 372 8.80e-168 474
Msa0074230 MtrunA17_Chr1g0211251 61.559 372 140 2 1 371 8 377 1.59e-164 466
Msa0074230 MtrunA17_Chr8g0371321 63.324 349 127 1 24 371 27 375 6.80e-164 464
Msa0074230 MtrunA17_Chr2g0294291 56.065 371 150 4 1 368 8 368 6.68e-146 417
Msa0074230 MtrunA17_Chr1g0175381 50.938 373 169 4 1 369 8 370 3.14e-129 376
Msa0074230 MtrunA17_Chr1g0207571 54.985 331 139 2 24 352 17 339 1.22e-121 356
Msa0074230 MtrunA17_Chr3g0089711 46.914 324 157 6 34 351 33 347 4.83e-97 293
Msa0074230 MtrunA17_Chr3g0089721 46.914 324 157 6 34 351 33 347 9.63e-97 292
Msa0074230 MtrunA17_Chr7g0256751 48.000 325 152 7 34 351 34 348 8.37e-91 280
Msa0074230 MtrunA17_Chr7g0256771 44.853 272 139 4 34 303 35 297 5.67e-72 227
Msa0074230 MtrunA17_Chr3g0089691 46.559 247 125 3 110 351 17 261 3.85e-71 224
Msa0074230 MtrunA17_Chr4g0063111 36.858 331 190 6 27 353 34 349 1.38e-62 209
Msa0074230 MtrunA17_Chr4g0063051 34.545 330 170 12 30 353 22 311 8.11e-46 160
Msa0074230 MtrunA17_Chr7g0238501 30.100 299 180 6 75 354 262 550 4.77e-41 152
Msa0074230 MtrunA17_Chr4g0039061 31.148 305 182 7 75 365 201 491 1.91e-39 146
Msa0074230 MtrunA17_Chr2g0295991 30.696 316 173 6 76 350 166 476 9.62e-39 144
Msa0074230 MtrunA17_Chr4g0031521 32.852 277 144 5 115 354 428 699 2.69e-37 142
Msa0074230 MtrunA17_Chr4g0067301 33.469 245 149 4 115 350 248 487 1.02e-35 136
Msa0074230 MtrunA17_Chr7g0256761 40.609 197 94 5 164 355 7 185 9.96e-35 126
Msa0074230 MtrunA17_Chr1g0193691 29.801 302 147 5 113 354 591 887 7.34e-34 133
Msa0074230 MtrunA17_Chr4g0054811 29.206 315 164 6 101 361 469 778 1.71e-32 129
Msa0074230 MtrunA17_Chr3g0105371 29.268 287 147 8 69 354 111 342 1.24e-27 111
Msa0074230 MtrunA17_Chr5g0430481 30.769 247 122 7 76 314 154 359 1.81e-27 111
Msa0074230 MtrunA17_Chr3g0105351 29.123 285 146 9 69 352 87 316 9.07e-27 108
Msa0074230 MtrunA17_Chr5g0407161 29.787 282 127 9 76 348 124 343 1.43e-24 103
Msa0074230 MtrunA17_Chr1g0150451 30.224 268 137 8 52 315 88 309 2.08e-23 99.8
Msa0074230 MtrunA17_Chr3g0136711 28.179 291 159 8 52 338 45 289 3.29e-23 98.2
Msa0074230 MtrunA17_Chr1g0212961 29.278 263 138 8 52 313 106 321 5.61e-23 98.6
Msa0074230 MtrunA17_Chr4g0062951 32.609 184 114 5 110 284 1 183 1.19e-22 94.0
Msa0074230 MtrunA17_Chr4g0050381 29.682 283 150 10 40 319 30 266 3.62e-22 95.1
Msa0074230 MtrunA17_Chr2g0317021 27.900 319 161 11 35 350 80 332 1.28e-21 94.4
Msa0074230 MtrunA17_Chr5g0426141 28.481 316 156 12 36 348 31 279 1.36e-21 93.6
Msa0074230 MtrunA17_Chr3g0115851 27.532 316 159 10 36 348 31 279 1.68e-21 93.2
Msa0074230 MtrunA17_Chr2g0279151 29.555 247 130 8 76 319 65 270 2.87e-21 92.4
Msa0074230 MtrunA17_Chr1g0171571 29.720 286 129 13 77 350 419 644 1.54e-20 93.6
Msa0074230 MtrunA17_Chr3g0126511 26.210 248 132 7 53 296 106 306 4.28e-20 90.5
Msa0074230 MtrunA17_Chr3g0144111 28.276 290 120 8 66 348 17 225 1.61e-19 87.0
Msa0074230 MtrunA17_Chr4g0070871 28.980 245 120 6 65 296 242 445 7.58e-19 87.8
Msa0074230 MtrunA17_Chr3g0135961 27.586 290 159 9 76 350 286 539 1.54e-18 87.0
Msa0074230 MtrunA17_Chr8g0360351 26.498 317 164 12 35 348 29 279 5.72e-18 83.2
Msa0074230 MtrunA17_Chr1g0194191 29.151 271 132 12 67 315 103 335 1.55e-17 83.2
Msa0074230 MtrunA17_Chr4g0001761 25.887 282 143 9 57 298 33 288 3.24e-17 82.0
Msa0074230 MtrunA17_Chr2g0293811 27.551 294 136 10 75 351 99 332 3.25e-17 82.0
Msa0074230 MtrunA17_Chr3g0112291 25.253 297 156 9 76 350 123 375 1.35e-16 80.5
Msa0074230 MtrunA17_Chr3g0138281 27.341 267 143 10 60 315 105 331 1.72e-16 80.1
Msa0074230 MtrunA17_Chr4g0051661 26.165 279 135 11 49 313 160 381 1.81e-16 80.1
Msa0074230 MtrunA17_Chr5g0435721 26.809 235 127 6 73 297 220 419 7.76e-16 79.0
Msa0074230 MtrunA17_Chr4g0065141 27.500 280 143 9 75 348 123 348 1.44e-15 77.4
Msa0074230 MtrunA17_Chr3g0136811 24.823 282 146 9 57 298 33 288 1.69e-15 77.0
Msa0074230 MtrunA17_Chr1g0158781 27.215 316 184 10 40 354 113 383 1.82e-15 77.0
Msa0074230 MtrunA17_Chr1g0150071 25.095 263 131 8 75 297 51 287 3.76e-15 75.9
Msa0074230 MtrunA17_Chr7g0272061 26.198 313 159 12 76 354 91 365 9.99e-15 74.7
Msa0074230 MtrunA17_Chr1g0150781 27.181 298 159 12 72 350 285 543 1.19e-14 75.5
Msa0074230 MtrunA17_Chr7g0223641 27.354 223 117 6 140 350 7 196 2.06e-14 71.6
Msa0074230 MtrunA17_Chr7g0221691 29.825 285 144 16 68 314 120 386 8.73e-13 69.3
Msa0074230 MtrunA17_Chr3g0127091 27.236 246 141 9 75 300 88 315 1.10e-12 68.9
Msa0074230 MtrunA17_Chr4g0067711 28.929 280 132 14 76 321 91 337 2.13e-12 67.8
Msa0074230 MtrunA17_Chr2g0295401 28.477 302 147 12 78 351 94 354 5.15e-12 66.6
Msa0074230 MtrunA17_Chr8g0389921 27.181 298 164 12 63 353 186 437 7.51e-12 66.6
Msa0074230 MtrunA17_Chr6g0483411 27.519 258 136 9 77 316 80 304 1.14e-11 65.1
Msa0074230 MtrunA17_Chr1g0192421 27.575 301 155 14 76 351 89 351 2.00e-11 64.7
Msa0074230 MtrunA17_Chr8g0342431 30.723 166 85 5 157 315 210 352 3.28e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074230 AT3G12620.2 66.667 372 120 2 1 371 10 378 0.0 514
Msa0074230 AT3G12620.1 66.667 372 120 2 1 371 10 378 0.0 514
Msa0074230 AT3G55050.1 63.612 371 131 1 1 371 9 375 1.58e-179 504
Msa0074230 AT3G55050.2 63.612 371 131 1 1 371 9 375 1.58e-179 504
Msa0074230 AT3G51370.1 65.805 348 117 1 24 371 27 372 7.81e-174 489
Msa0074230 AT3G51370.3 65.805 348 117 1 24 371 27 372 7.81e-174 489
Msa0074230 AT5G66080.1 65.714 350 118 2 24 371 29 378 2.01e-171 483
Msa0074230 AT4G38520.2 60.484 372 144 2 1 371 8 377 1.12e-165 469
Msa0074230 AT4G38520.1 60.484 372 144 2 1 371 8 377 1.12e-165 469
Msa0074230 AT5G02760.1 60.811 370 138 3 1 370 1 363 1.23e-155 442
Msa0074230 AT3G17090.1 59.451 328 123 3 29 355 45 363 7.44e-137 395
Msa0074230 AT5G66080.2 66.296 270 90 1 103 371 5 274 8.54e-131 376
Msa0074230 AT3G51370.2 65.056 269 92 1 103 371 21 287 1.00e-129 374
Msa0074230 AT5G06750.3 52.035 344 154 2 29 369 43 378 4.55e-122 358
Msa0074230 AT5G06750.1 52.035 344 154 2 29 369 43 378 4.55e-122 358
Msa0074230 AT4G33920.1 50.420 357 161 4 21 373 14 358 9.08e-115 339
Msa0074230 AT3G17090.2 56.569 274 109 3 29 301 45 309 2.08e-106 315
Msa0074230 AT5G06750.2 52.963 270 124 1 103 369 27 296 1.06e-94 285
Msa0074230 AT4G33920.2 50.903 277 130 2 100 373 2 275 3.32e-89 271
Msa0074230 AT4G33920.3 51.210 248 115 2 129 373 1 245 8.19e-80 246
Msa0074230 AT3G16560.1 29.808 312 176 7 76 350 175 480 2.04e-38 144
Msa0074230 AT3G16560.4 29.808 312 176 7 76 350 175 480 2.04e-38 144
Msa0074230 AT3G16560.2 29.808 312 176 7 76 350 175 480 2.04e-38 144
Msa0074230 AT3G09400.1 32.979 282 143 8 115 365 384 650 6.38e-38 144
Msa0074230 AT5G02400.1 32.509 283 146 6 117 365 403 674 5.45e-37 142
Msa0074230 AT1G07630.1 31.769 277 147 5 115 354 386 657 9.53e-37 141
Msa0074230 AT2G28890.1 31.655 278 148 5 114 354 377 649 1.91e-36 140
Msa0074230 AT2G46920.3 29.470 302 147 5 114 354 543 839 3.99e-35 137
Msa0074230 AT2G46920.1 29.470 302 147 5 114 354 543 839 3.99e-35 137
Msa0074230 AT2G46920.2 29.470 302 147 5 114 354 543 839 3.99e-35 137
Msa0074230 AT2G46920.4 29.470 302 147 5 114 354 543 839 3.99e-35 137
Msa0074230 AT1G07160.1 32.862 283 135 8 72 352 149 378 1.66e-33 128
Msa0074230 AT2G35350.1 29.293 297 154 6 114 364 493 779 8.27e-33 130
Msa0074230 AT3G16560.3 29.078 282 157 7 76 320 175 450 2.13e-29 118
Msa0074230 AT2G40180.1 29.412 289 144 9 71 354 156 389 6.83e-28 113
Msa0074230 AT3G09400.2 32.068 237 121 7 115 320 384 611 1.15e-27 115
Msa0074230 AT2G30020.1 30.742 283 134 9 75 352 169 394 3.38e-27 111
Msa0074230 AT1G67820.2 33.480 227 108 5 73 297 112 297 6.89e-26 108
Msa0074230 AT1G67820.1 33.480 227 108 5 73 297 148 333 8.01e-26 108
Msa0074230 AT4G31750.2 30.996 271 138 8 52 318 44 269 1.66e-25 105
Msa0074230 AT4G31750.1 30.996 271 138 8 52 318 44 269 2.06e-25 105
Msa0074230 AT4G31750.3 30.996 271 138 8 52 318 44 269 2.06e-25 105
Msa0074230 AT3G51470.2 31.727 249 124 7 74 315 60 269 8.97e-25 103
Msa0074230 AT3G51470.1 31.727 249 124 7 74 315 104 313 1.41e-24 103
Msa0074230 AT2G33700.1 31.973 294 127 9 68 348 109 342 3.86e-24 102
Msa0074230 AT5G10740.1 29.225 284 149 9 36 315 30 265 3.85e-22 96.7
Msa0074230 AT5G10740.2 29.225 284 149 9 36 315 30 265 3.85e-22 96.7
Msa0074230 AT1G43900.1 30.714 280 130 11 71 348 149 366 4.83e-22 96.7
Msa0074230 AT4G28400.3 28.205 273 150 10 49 319 22 250 8.33e-22 94.0
Msa0074230 AT4G28400.1 28.205 273 150 10 49 319 44 272 1.45e-21 94.0
Msa0074230 AT5G24940.1 28.689 244 128 7 76 315 64 265 4.70e-21 94.7
Msa0074230 AT2G20630.1 27.985 268 147 10 49 314 40 263 2.73e-20 90.1
Msa0074230 AT2G20630.2 27.985 268 147 10 49 314 40 263 5.45e-20 89.4
Msa0074230 AT3G62260.3 31.660 259 130 9 69 314 20 244 6.51e-20 89.4
Msa0074230 AT3G62260.1 31.274 259 131 9 69 314 109 333 2.30e-19 89.0
Msa0074230 AT3G62260.2 31.274 259 131 9 69 314 110 334 2.43e-19 89.0
Msa0074230 AT5G53140.1 24.150 294 158 8 76 366 132 363 3.12e-19 89.0
Msa0074230 AT5G53140.2 24.150 294 158 8 76 366 132 363 3.12e-19 89.0
Msa0074230 AT1G78200.1 27.097 310 163 11 40 348 33 280 4.64e-19 86.7
Msa0074230 AT1G78200.3 27.097 310 163 11 40 348 33 280 4.64e-19 86.7
Msa0074230 AT1G78200.2 27.097 310 163 11 40 348 33 280 4.64e-19 86.7
Msa0074230 AT1G34750.2 26.861 309 161 11 43 350 37 281 9.05e-19 85.9
Msa0074230 AT1G34750.1 26.861 309 161 11 43 350 37 281 9.05e-19 85.9
Msa0074230 AT1G34750.3 26.861 309 161 11 43 350 37 281 9.05e-19 85.9
Msa0074230 AT3G15260.2 28.013 307 156 11 43 348 44 286 4.42e-18 84.0
Msa0074230 AT3G15260.1 28.013 307 156 11 43 348 44 286 4.42e-18 84.0
Msa0074230 AT4G26080.1 26.144 306 176 10 62 353 156 425 5.48e-18 85.5
Msa0074230 AT1G22280.3 27.645 293 161 10 34 319 28 276 6.15e-18 83.6
Msa0074230 AT4G08260.1 26.756 299 130 11 54 352 1 210 9.41e-18 81.6
Msa0074230 AT2G25620.1 27.157 313 166 13 48 350 96 356 1.32e-17 84.0
Msa0074230 AT2G25620.2 27.157 313 166 13 48 350 96 356 1.32e-17 84.0
Msa0074230 AT2G25070.1 25.887 282 143 9 57 298 33 288 2.03e-17 83.2
Msa0074230 AT2G25070.2 25.887 282 143 9 57 298 33 288 2.03e-17 83.2
Msa0074230 AT1G78200.5 26.502 283 150 9 67 348 10 235 2.74e-17 80.9
Msa0074230 AT1G78200.4 26.502 283 150 9 67 348 10 235 2.74e-17 80.9
Msa0074230 AT1G22280.1 27.431 288 162 9 34 319 28 270 5.59e-17 80.9
Msa0074230 AT4G31860.3 25.795 283 142 10 57 298 33 288 1.92e-16 79.3
Msa0074230 AT2G40860.4 28.622 283 137 11 76 350 325 550 3.49e-16 80.5
Msa0074230 AT1G07430.1 26.180 233 126 7 76 297 157 354 3.62e-16 80.1
Msa0074230 AT2G40860.1 28.622 283 137 11 76 350 423 648 5.89e-16 80.1
Msa0074230 AT4G31860.1 25.795 283 142 10 57 298 33 288 7.62e-16 78.6
Msa0074230 AT1G48040.1 27.019 322 163 12 44 348 81 347 1.60e-15 77.8
Msa0074230 AT2G34740.1 27.016 248 132 11 49 293 96 297 2.11e-15 77.0
Msa0074230 AT2G29380.1 27.917 240 119 9 72 296 109 309 2.68e-15 77.0
Msa0074230 AT1G72770.4 27.469 324 144 13 66 350 230 501 2.99e-15 77.4
Msa0074230 AT1G72770.1 27.469 324 144 13 66 350 230 501 2.99e-15 77.4
Msa0074230 AT1G72770.3 27.469 324 144 13 66 350 230 501 2.99e-15 77.4
Msa0074230 AT2G40860.2 28.800 250 133 10 76 316 423 636 3.37e-15 77.4
Msa0074230 AT4G28400.2 27.615 239 128 9 49 286 44 238 3.88e-15 75.1
Msa0074230 AT5G51760.2 28.750 240 120 7 76 296 114 321 4.06e-15 76.6
Msa0074230 AT5G51760.1 28.750 240 120 7 76 296 144 351 4.86e-15 76.6
Msa0074230 AT5G27930.1 26.733 303 152 15 76 349 92 353 5.50e-15 75.9
Msa0074230 AT5G27930.2 26.733 303 152 15 76 349 92 353 5.50e-15 75.9
Msa0074230 AT1G17550.1 26.971 241 123 8 76 295 237 445 6.13e-15 76.6
Msa0074230 AT1G17550.2 26.971 241 123 8 76 295 237 445 6.13e-15 76.6
Msa0074230 AT3G05640.2 27.946 297 154 12 76 351 93 350 7.80e-15 75.5
Msa0074230 AT3G05640.1 27.946 297 154 12 76 351 93 350 7.80e-15 75.5
Msa0074230 AT3G05640.3 27.946 297 154 12 76 351 93 350 7.80e-15 75.5
Msa0074230 AT5G59220.1 27.706 231 122 7 76 297 148 342 9.03e-15 75.5
Msa0074230 AT5G57050.2 24.561 285 174 8 76 351 120 372 1.04e-14 75.5
Msa0074230 AT5G57050.1 24.561 285 174 8 76 351 160 412 1.05e-14 75.5
Msa0074230 AT3G17250.1 27.076 277 139 12 67 319 142 379 3.24e-14 73.9
Msa0074230 AT4G31860.2 26.296 270 131 10 57 285 33 275 4.56e-14 72.4
Msa0074230 AT3G16800.2 27.119 295 158 10 75 350 92 348 4.75e-14 73.2
Msa0074230 AT3G16800.1 27.119 295 158 10 75 350 92 348 4.75e-14 73.2
Msa0074230 AT3G16800.3 27.119 295 158 10 75 350 92 348 4.75e-14 73.2
Msa0074230 AT5G26010.1 28.231 294 156 13 76 353 73 327 9.86e-14 72.0
Msa0074230 AT2G34740.2 26.556 241 128 11 49 286 110 304 1.42e-13 71.2
Msa0074230 AT2G34740.3 26.556 241 128 11 49 286 92 286 1.72e-13 70.9
Msa0074230 AT1G34750.4 25.306 245 138 7 43 286 37 237 4.66e-13 68.9
Msa0074230 AT5G01700.2 24.834 302 164 13 76 353 78 340 7.56e-13 69.7
Msa0074230 AT5G01700.1 24.834 302 164 13 76 353 29 291 7.95e-13 69.3
Msa0074230 AT4G32950.1 26.756 299 164 13 69 353 62 319 1.25e-12 68.6
Msa0074230 AT3G16800.4 28.448 232 124 7 127 350 56 253 7.31e-12 65.5
Msa0074230 AT3G16800.6 28.448 232 124 7 127 350 56 253 7.31e-12 65.5

Find 96 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTATGGTACAAAGATTT+AGG 0.145257 1_2:-48540325 Msa0074230:CDS
GATTTAGGGAACCATATTTA+TGG 0.226833 1_2:-48540310 Msa0074230:CDS
TTGAAAATTCACCATAAATA+TGG 0.240008 1_2:+48540299 None:intergenic
ACCTTTGAGTTCTAACCATT+TGG 0.248033 1_2:-48540221 Msa0074230:CDS
TGATTGATAACAGCTCTCTT+TGG 0.269072 1_2:-48538198 Msa0074230:CDS
TATTTGCTTCTGATGGGTTA+TGG 0.270609 1_2:-48538902 Msa0074230:CDS
GATTGATAACAGCTCTCTTT+GGG 0.317947 1_2:-48538197 Msa0074230:CDS
ATTTGCTTCTGATGGGTTAT+GGG 0.319833 1_2:-48538901 Msa0074230:CDS
TGAAGAAAGCAGAATTCAAT+AGG 0.336510 1_2:-48539034 Msa0074230:CDS
CTGCTATATATTGCAAATTC+TGG 0.336609 1_2:-48539389 Msa0074230:CDS
CCTTTGAGTTCTAACCATTT+GGG 0.343999 1_2:-48540220 Msa0074230:CDS
ACCAAATTTGTACCTGTATG+AGG 0.357900 1_2:+48539192 None:intergenic
CAGAAGCAAATATGAGAAAT+TGG 0.359419 1_2:+48538913 None:intergenic
TGTTATGGTACAAAGATTTA+GGG 0.362538 1_2:-48540324 Msa0074230:CDS
GGGAGTGGTGGGAGAGTTGA+TGG 0.367545 1_2:-48540349 Msa0074230:CDS
ACAATTGTCTAAAATTTGAT+TGG 0.372658 1_2:+48538055 None:intergenic
GTTCATCTCTCACTCTTTCA+AGG 0.384829 1_2:+48539282 None:intergenic
TCAGCAAGTCTGAACTTTGA+TGG 0.386663 1_2:+48539002 None:intergenic
TTTCTCATATTTGCTTCTGA+TGG 0.398381 1_2:-48538909 Msa0074230:CDS
AATATGAGAAATTGGTCATC+TGG 0.401453 1_2:+48538921 None:intergenic
ATCAGCAGGTACTTGTTGTT+TGG 0.418296 1_2:-48539432 Msa0074230:CDS
GATGGCTCACAGCTAAGAAT+TGG 0.420072 1_2:+48538966 None:intergenic
GATGGGAGTGGAAGTGGGAG+TGG 0.421706 1_2:-48540364 Msa0074230:CDS
AGCAGGTACTTGTTGTTTGG+TGG 0.424999 1_2:-48539429 Msa0074230:CDS
ACCACAATTTGTGAATCATA+AGG 0.427794 1_2:+48539245 None:intergenic
TGTCGGAAAATGTGATCAAA+AGG 0.429591 1_2:-48539526 Msa0074230:CDS
GATGGAGATGGGAGTGGAAG+TGG 0.429908 1_2:-48540370 Msa0074230:CDS
GATTCAAGCTAATAGTTCAT+TGG 0.431739 1_2:-48540269 Msa0074230:CDS
CCCTTATGATTCACAAATTG+TGG 0.437712 1_2:-48539246 Msa0074230:CDS
GGTGACTCAAGGGTGGTGTT+AGG 0.443325 1_2:-48539368 Msa0074230:CDS
GCAGGTACTTGTTGTTTGGT+GGG 0.444107 1_2:-48539428 Msa0074230:CDS
ATGAAGTTTATGTGATGAAA+TGG 0.445727 1_2:+48538944 None:intergenic
TAATCCACCAGATCATAAAC+TGG 0.453417 1_2:+48537968 None:intergenic
GATAGAAGTGAATTTGAGTC+TGG 0.454463 1_2:-48540244 Msa0074230:CDS
CCACAATTTGTGAATCATAA+GGG 0.455049 1_2:+48539246 None:intergenic
GTTCTGTCCAGTTTATGATC+TGG 0.459600 1_2:-48537975 Msa0074230:three_prime_UTR
GGGAGTGGAAGTGGGAGTGG+TGG 0.471115 1_2:-48540361 Msa0074230:CDS
TCTCTTTCAATCAAAGGAGG+TGG 0.472096 1_2:-48538169 Msa0074230:CDS
TTCTCATATTTGCTTCTGAT+GGG 0.482026 1_2:-48538908 Msa0074230:CDS
GGAGAGTTGATGGATTGTTA+TGG 0.482127 1_2:-48540339 Msa0074230:CDS
AAAAGATGGTGAGATCTTGC+TGG 0.490229 1_2:-48540414 None:intergenic
GGGCTTGCAGCTGAAAATCA+AGG 0.497382 1_2:-48539551 Msa0074230:CDS
CACAATGTTTGGCGTGTCAA+AGG 0.498022 1_2:-48539215 Msa0074230:CDS
GGAGTGGAAGTGGGAGTGGT+GGG 0.499352 1_2:-48540360 Msa0074230:CDS
ATGTTCTGCAGATAATTGTA+TGG 0.500987 1_2:+48539313 None:intergenic
TTGCAGGTTTCGCGATCGAT+CGG 0.503749 1_2:-48539068 Msa0074230:intron
AAATGAAGCCTTACATTAGG+TGG 0.504260 1_2:+48538834 None:intergenic
TGGTTATGAGACACAATGTT+TGG 0.504918 1_2:-48539226 Msa0074230:CDS
TGATCAATCAACTTAGTGAT+TGG 0.507620 1_2:+48538141 None:intergenic
CCATCATCACCATCTGCAGC+TGG 0.507878 1_2:+48540388 None:intergenic
TTGCAAATTCTGGTGACTCA+AGG 0.512676 1_2:-48539379 Msa0074230:CDS
AGCTGAAAATCAAGGCTTGT+CGG 0.513050 1_2:-48539543 Msa0074230:CDS
ATTGGTGTTTATGATGGTCA+TGG 0.514731 1_2:-48540181 Msa0074230:CDS
GGTTCTTCTCTTTCAATCAA+AGG 0.518546 1_2:-48538175 Msa0074230:CDS
CAGGTACTTGTTGTTTGGTG+GGG 0.520451 1_2:-48539427 Msa0074230:CDS
CCAGCTGCAGATGGTGATGA+TGG 0.524607 1_2:-48540388 Msa0074230:CDS
CTGTCCAGTTTATGATCTGG+TGG 0.527868 1_2:-48537972 Msa0074230:three_prime_UTR
TGCAAATTCTGGTGACTCAA+GGG 0.528408 1_2:-48539378 Msa0074230:CDS
GAAGAAAGCAGAATTCAATA+GGG 0.530148 1_2:-48539033 Msa0074230:CDS
ATGGAGATGGGAGTGGAAGT+GGG 0.535272 1_2:-48540369 Msa0074230:CDS
AAGTCCCTTGAGGACCCAAA+TGG 0.544433 1_2:+48540206 None:intergenic
TCTAACCATTTGGGTCCTCA+AGG 0.545656 1_2:-48540211 Msa0074230:CDS
TGCTGGAAGCCAGCTGCAGA+TGG 0.549950 1_2:-48540397 Msa0074230:CDS
GCGTGTCAAAGGCCTCATAC+AGG 0.554102 1_2:-48539204 Msa0074230:intron
GGTGATGATGGAGATGGGAG+TGG 0.556141 1_2:-48540376 Msa0074230:CDS
AAACTGGACAGAACAAGGTA+GGG 0.557135 1_2:+48537984 None:intergenic
TCAAGCTAATAGTTCATTGG+AGG 0.562094 1_2:-48540266 Msa0074230:CDS
TAAACTGGACAGAACAAGGT+AGG 0.564051 1_2:+48537983 None:intergenic
CAGCCACATATTGCATCAGC+AGG 0.566567 1_2:-48539446 Msa0074230:CDS
CAATCACAGTTATATCATCA+TGG 0.569267 1_2:+48538242 None:intergenic
ATCATAAACTGGACAGAACA+AGG 0.576122 1_2:+48537979 None:intergenic
GTCGGAAAATGTGATCAAAA+GGG 0.584435 1_2:-48539525 Msa0074230:CDS
TCTTCTCTTTCAATCAAAGG+AGG 0.590775 1_2:-48538172 Msa0074230:CDS
AAGACTAGAGAGGGCAACAA+GGG 0.596221 1_2:-48539345 Msa0074230:CDS
GCAGATGGTGATGATGGAGA+TGG 0.599305 1_2:-48540382 Msa0074230:CDS
CTAACCATTTGGGTCCTCAA+GGG 0.601292 1_2:-48540210 Msa0074230:CDS
ATTGATAACAGCTCTCTTTG+GGG 0.611908 1_2:-48538196 Msa0074230:CDS
AGTCTGAACTTTGATGGTAG+AGG 0.612065 1_2:+48539008 None:intergenic
GAAGACTAGAGAGGGCAACA+AGG 0.614407 1_2:-48539346 Msa0074230:CDS
CCCAAATGGTTAGAACTCAA+AGG 0.617302 1_2:+48540220 None:intergenic
AACTGGACAGAACAAGGTAG+GGG 0.621101 1_2:+48537985 None:intergenic
GGTGTTAGGAAGACTAGAGA+GGG 0.624868 1_2:-48539354 Msa0074230:CDS
CAGATGGTGATGATGGAGAT+GGG 0.625666 1_2:-48540381 Msa0074230:CDS
TTGGTGGGGATAATTTGCAA+TGG 0.626466 1_2:-48539413 Msa0074230:CDS
CAGCACTCAGAGAAGCAGCA+AGG 0.626568 1_2:-48538324 Msa0074230:CDS
AAAAGATTGAACAAGGAACA+AGG 0.630842 1_2:-48538273 Msa0074230:CDS
TGGTGTTAGGAAGACTAGAG+AGG 0.638450 1_2:-48539355 Msa0074230:CDS
GACCTTCAAAAGATTGAACA+AGG 0.644560 1_2:-48538280 Msa0074230:CDS
GGAGCAGCTTAGCAATCAAG+AGG 0.645253 1_2:-48538880 Msa0074230:CDS
TATCACTTGTGAATAAACAG+TGG 0.649816 1_2:-48539475 Msa0074230:CDS
GTACCTGCTGATGCAATATG+TGG 0.654955 1_2:+48539443 None:intergenic
GGTGTTTATGATGGTCATGG+TGG 0.668522 1_2:-48540178 Msa0074230:CDS
AGTTTATGTGATGAAATGGA+TGG 0.689072 1_2:+48538948 None:intergenic
ACACCAATAAAAGTCCCTTG+AGG 0.707591 1_2:+48540196 None:intergenic
AAATTCTGGTGACTCAAGGG+TGG 0.717797 1_2:-48539375 Msa0074230:CDS
ACTGGACAGAACAAGGTAGG+GGG 0.718372 1_2:+48537986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATTTATTTTTTGTTG+AGG + chr1_2:48538616-48538635 None:intergenic 10.0%
!! AATAATAAACTCAAAATAAA+GGG + chr1_2:48538679-48538698 None:intergenic 10.0%
!! TAATAATAAACTCAAAATAA+AGG + chr1_2:48538680-48538699 None:intergenic 10.0%
!! GTTCAAATAAATTAATTTAA+AGG + chr1_2:48539676-48539695 None:intergenic 10.0%
!!! ATTTTTTTTTTTTTTTTGAG+AGG + chr1_2:48540249-48540268 None:intergenic 10.0%
!!! TATATATATAAGATTTTCAA+TGG + chr1_2:48540366-48540385 None:intergenic 10.0%
!!! TATATATATGAATTTTTCTT+TGG - chr1_2:48540392-48540411 Msa0074230:CDS 10.0%
!! TATGAAAATCTATATAGTTA+TGG + chr1_2:48538197-48538216 None:intergenic 15.0%
!!! ATTTTGAAAATATACATAAG+AGG + chr1_2:48538250-48538269 None:intergenic 15.0%
!!! TATATTTTCAAAATAACTGA+GGG - chr1_2:48538256-48538275 Msa0074230:CDS 15.0%
!!! CAGGAATTATAAAATTTTTA+CGG + chr1_2:48539126-48539145 None:intergenic 15.0%
!!! ATGTAGTTTTGTAAATTTTA+AGG + chr1_2:48539183-48539202 None:intergenic 15.0%
!! AATTTATTTGAACTTATCTA+CGG - chr1_2:48539683-48539702 Msa0074230:intron 15.0%
!! TTGAAAATTCACCATAAATA+TGG + chr1_2:48537980-48537999 None:intergenic 20.0%
!!! TATTTATGGTGAATTTTCAA+TGG - chr1_2:48537980-48537999 Msa0074230:three_prime_UTR 20.0%
!!! GTATATTTTCAAAATAACTG+AGG - chr1_2:48538255-48538274 Msa0074230:CDS 20.0%
!!! AAATAAGCATTTTATGTAAC+AGG - chr1_2:48539905-48539924 Msa0074230:intron 20.0%
!!! AATAAGCATTTTATGTAACA+GGG - chr1_2:48539906-48539925 Msa0074230:intron 20.0%
!!! ACAATTGTCTAAAATTTGAT+TGG + chr1_2:48540224-48540243 None:intergenic 20.0%
! TTGTTATGGTACAAAGATTT+AGG - chr1_2:48537951-48537970 Msa0074230:three_prime_UTR 25.0%
! TGTTATGGTACAAAGATTTA+GGG - chr1_2:48537952-48537971 Msa0074230:three_prime_UTR 25.0%
!!! ACTTTTATTGGTGTTTATGA+TGG - chr1_2:48538089-48538108 Msa0074230:three_prime_UTR 25.0%
! TTAATCTAACAGTCTATAGT+CGG - chr1_2:48538487-48538506 Msa0074230:intron 25.0%
!!! GTAAATTTTAAGGACTAAAG+TGG + chr1_2:48539173-48539192 None:intergenic 25.0%
! ATGAAGTTTATGTGATGAAA+TGG + chr1_2:48539335-48539354 None:intergenic 25.0%
! ATAAAATGAAGCCTTACATT+AGG + chr1_2:48539448-48539467 None:intergenic 25.0%
!!! CATTTTATTTTTGCCATTCA+AGG - chr1_2:48539460-48539479 Msa0074230:CDS 25.0%
! ATGATAACAATAACCTTGAA+TGG + chr1_2:48539476-48539495 None:intergenic 25.0%
! ACAACTTAAGGATATATCAT+CGG + chr1_2:48539724-48539743 None:intergenic 25.0%
!!! GTTGTTTTCATTACTTATCA+TGG + chr1_2:48539770-48539789 None:intergenic 25.0%
! ATAATCACTTATCATGTAAG+TGG + chr1_2:48539855-48539874 None:intergenic 25.0%
!! GAACATATATTTTGTACAGA+TGG - chr1_2:48540335-48540354 Msa0074230:CDS 25.0%
!! ATTTTTCTTTGGCAAAAGTA+AGG - chr1_2:48540403-48540422 Msa0074230:CDS 25.0%
GATTTAGGGAACCATATTTA+TGG - chr1_2:48537966-48537985 Msa0074230:three_prime_UTR 30.0%
GATTCAAGCTAATAGTTCAT+TGG - chr1_2:48538007-48538026 Msa0074230:three_prime_UTR 30.0%
TTTCAAAATAACTGAGGGTT+AGG - chr1_2:48538261-48538280 Msa0074230:CDS 30.0%
TTCAAAATAACTGAGGGTTA+GGG - chr1_2:48538262-48538281 Msa0074230:CDS 30.0%
AAAAAAATCACTCAAGGTGT+TGG + chr1_2:48538404-48538423 None:intergenic 30.0%
CAGGTTAAAAAAATCACTCA+AGG + chr1_2:48538410-48538429 None:intergenic 30.0%
TAATCTAACAGTCTATAGTC+GGG - chr1_2:48538488-48538507 Msa0074230:intron 30.0%
! TCAATTTTCTTTGTGATCCA+GGG - chr1_2:48538705-48538724 Msa0074230:intron 30.0%
TATCACTTGTGAATAAACAG+TGG - chr1_2:48538801-48538820 Msa0074230:intron 30.0%
CTGCTATATATTGCAAATTC+TGG - chr1_2:48538887-48538906 Msa0074230:CDS 30.0%
ATGTTCTGCAGATAATTGTA+TGG + chr1_2:48538966-48538985 None:intergenic 30.0%
CCACAATTTGTGAATCATAA+GGG + chr1_2:48539033-48539052 None:intergenic 30.0%
ACCACAATTTGTGAATCATA+AGG + chr1_2:48539034-48539053 None:intergenic 30.0%
TGGATACAAAGACTAATAAC+AGG + chr1_2:48539145-48539164 None:intergenic 30.0%
TGAAGAAAGCAGAATTCAAT+AGG - chr1_2:48539242-48539261 Msa0074230:CDS 30.0%
GAAGAAAGCAGAATTCAATA+GGG - chr1_2:48539243-48539262 Msa0074230:CDS 30.0%
AGTTTATGTGATGAAATGGA+TGG + chr1_2:48539331-48539350 None:intergenic 30.0%
AATATGAGAAATTGGTCATC+TGG + chr1_2:48539358-48539377 None:intergenic 30.0%
CAGAAGCAAATATGAGAAAT+TGG + chr1_2:48539366-48539385 None:intergenic 30.0%
TTTCTCATATTTGCTTCTGA+TGG - chr1_2:48539367-48539386 Msa0074230:CDS 30.0%
TTCTCATATTTGCTTCTGAT+GGG - chr1_2:48539368-48539387 Msa0074230:CDS 30.0%
CTTTCACATTATTGCAAATC+TGG - chr1_2:48539501-48539520 Msa0074230:CDS 30.0%
TTAATCCGTCCAATTAAGAT+CGG + chr1_2:48539594-48539613 None:intergenic 30.0%
! GACTAACTTTTGGTATACTA+TGG - chr1_2:48539649-48539668 Msa0074230:intron 30.0%
AATCAGCTGAAAACAACTTA+AGG + chr1_2:48539736-48539755 None:intergenic 30.0%
! TTCCTTGTTCAATCTTTTGA+AGG + chr1_2:48540001-48540020 None:intergenic 30.0%
AAAAGATTGAACAAGGAACA+AGG - chr1_2:48540003-48540022 Msa0074230:intron 30.0%
CAATCACAGTTATATCATCA+TGG + chr1_2:48540037-48540056 None:intergenic 30.0%
TGATCAATCAACTTAGTGAT+TGG + chr1_2:48540138-48540157 None:intergenic 30.0%
TCAAGCTAATAGTTCATTGG+AGG - chr1_2:48538010-48538029 Msa0074230:three_prime_UTR 35.0%
GATAGAAGTGAATTTGAGTC+TGG - chr1_2:48538032-48538051 Msa0074230:three_prime_UTR 35.0%
! ACCTTTGAGTTCTAACCATT+TGG - chr1_2:48538055-48538074 Msa0074230:three_prime_UTR 35.0%
! CCTTTGAGTTCTAACCATTT+GGG - chr1_2:48538056-48538075 Msa0074230:three_prime_UTR 35.0%
! ATTGGTGTTTATGATGGTCA+TGG - chr1_2:48538095-48538114 Msa0074230:three_prime_UTR 35.0%
AGGGAAATTTGTAGTCTCTT+CGG - chr1_2:48538281-48538300 Msa0074230:CDS 35.0%
CCTTTAGATTAATCCAACGA+CGG + chr1_2:48538315-48538334 None:intergenic 35.0%
TTAATCTAAAGGCTCGTGTT+TGG - chr1_2:48538323-48538342 Msa0074230:CDS 35.0%
AATCTAACAATCTACACAGC+CGG + chr1_2:48538445-48538464 None:intergenic 35.0%
! GTCAATTTTCTTTGTGATCC+AGG - chr1_2:48538704-48538723 Msa0074230:intron 35.0%
TGTCGGAAAATGTGATCAAA+AGG - chr1_2:48538750-48538769 Msa0074230:intron 35.0%
GTCGGAAAATGTGATCAAAA+GGG - chr1_2:48538751-48538770 Msa0074230:intron 35.0%
CCCTTATGATTCACAAATTG+TGG - chr1_2:48539030-48539049 Msa0074230:CDS 35.0%
TGGTTATGAGACACAATGTT+TGG - chr1_2:48539050-48539069 Msa0074230:CDS 35.0%
ACCAAATTTGTACCTGTATG+AGG + chr1_2:48539087-48539106 None:intergenic 35.0%
TAAGGACTAAAGTGGAATAG+TGG + chr1_2:48539165-48539184 None:intergenic 35.0%
!! CAAAACTACATGCTTTTTGC+AGG - chr1_2:48539192-48539211 Msa0074230:intron 35.0%
!! TATTTGCTTCTGATGGGTTA+TGG - chr1_2:48539374-48539393 Msa0074230:CDS 35.0%
!! ATTTGCTTCTGATGGGTTAT+GGG - chr1_2:48539375-48539394 Msa0074230:CDS 35.0%
ACAACAATCCACCTAATGTA+AGG - chr1_2:48539434-48539453 Msa0074230:CDS 35.0%
AAATGAAGCCTTACATTAGG+TGG + chr1_2:48539445-48539464 None:intergenic 35.0%
ACTGTTCGATCAAGATTTGA+CGG + chr1_2:48539528-48539547 None:intergenic 35.0%
! ACTGCATTCTGACTAACTTT+TGG - chr1_2:48539639-48539658 Msa0074230:intron 35.0%
TTACTTATCATGGAGAGCTA+TGG + chr1_2:48539760-48539779 None:intergenic 35.0%
GACCTTCAAAAGATTGAACA+AGG - chr1_2:48539996-48540015 Msa0074230:intron 35.0%
! TGATTGATAACAGCTCTCTT+TGG - chr1_2:48540078-48540097 Msa0074230:intron 35.0%
! GATTGATAACAGCTCTCTTT+GGG - chr1_2:48540079-48540098 Msa0074230:intron 35.0%
! ATTGATAACAGCTCTCTTTG+GGG - chr1_2:48540080-48540099 Msa0074230:intron 35.0%
GGTTCTTCTCTTTCAATCAA+AGG - chr1_2:48540101-48540120 Msa0074230:intron 35.0%
TCTTCTCTTTCAATCAAAGG+AGG - chr1_2:48540104-48540123 Msa0074230:intron 35.0%
ATCATAAACTGGACAGAACA+AGG + chr1_2:48540300-48540319 None:intergenic 35.0%
TAATCCACCAGATCATAAAC+TGG + chr1_2:48540311-48540330 None:intergenic 35.0%
!! GGAGAGTTGATGGATTGTTA+TGG - chr1_2:48537937-48537956 Msa0074230:three_prime_UTR 40.0%
CCCAAATGGTTAGAACTCAA+AGG + chr1_2:48538059-48538078 None:intergenic 40.0%
ACACCAATAAAAGTCCCTTG+AGG + chr1_2:48538083-48538102 None:intergenic 40.0%
TTAATCCAACGACGGATGTT+TGG + chr1_2:48538307-48538326 None:intergenic 40.0%
CCGTCGTTGGATTAATCTAA+AGG - chr1_2:48538312-48538331 Msa0074230:CDS 40.0%
TAACCTGAATCCTCTATAGC+CGG - chr1_2:48538423-48538442 Msa0074230:intron 40.0%
AGCTGAAAATCAAGGCTTGT+CGG - chr1_2:48538733-48538752 Msa0074230:intron 40.0%
!! ATCAGCAGGTACTTGTTGTT+TGG - chr1_2:48538844-48538863 Msa0074230:intron 40.0%
! TTGGTGGGGATAATTTGCAA+TGG - chr1_2:48538863-48538882 Msa0074230:CDS 40.0%
TTGCAAATTCTGGTGACTCA+AGG - chr1_2:48538897-48538916 Msa0074230:CDS 40.0%
TGCAAATTCTGGTGACTCAA+GGG - chr1_2:48538898-48538917 Msa0074230:CDS 40.0%
GTTCATCTCTCACTCTTTCA+AGG + chr1_2:48538997-48539016 None:intergenic 40.0%
GCCTCATACAGGTACAAATT+TGG - chr1_2:48539083-48539102 Msa0074230:intron 40.0%
! AGTCTGAACTTTGATGGTAG+AGG + chr1_2:48539271-48539290 None:intergenic 40.0%
TCAGCAAGTCTGAACTTTGA+TGG + chr1_2:48539277-48539296 None:intergenic 40.0%
AAATCTTGATCGAACAGTCC+AGG - chr1_2:48539530-48539549 Msa0074230:CDS 40.0%
GCAAAACAAGCTAAAACACC+TGG + chr1_2:48539551-48539570 None:intergenic 40.0%
GGATTAAATTGCGTGAATGC+AGG - chr1_2:48539607-48539626 Msa0074230:intron 40.0%
TCTCTTTCAATCAAAGGAGG+TGG - chr1_2:48540107-48540126 Msa0074230:intron 40.0%
AAACTGGACAGAACAAGGTA+GGG + chr1_2:48540295-48540314 None:intergenic 40.0%
TAAACTGGACAGAACAAGGT+AGG + chr1_2:48540296-48540315 None:intergenic 40.0%
GTTCTGTCCAGTTTATGATC+TGG - chr1_2:48540301-48540320 Msa0074230:CDS 40.0%
!! CAGATGGTGATGATGGAGAT+GGG - chr1_2:48537895-48537914 Msa0074230:three_prime_UTR 45.0%
TCTAACCATTTGGGTCCTCA+AGG - chr1_2:48538065-48538084 Msa0074230:three_prime_UTR 45.0%
CTAACCATTTGGGTCCTCAA+GGG - chr1_2:48538066-48538085 Msa0074230:three_prime_UTR 45.0%
! GGTCCTCAAGGGACTTTTAT+TGG - chr1_2:48538077-48538096 Msa0074230:three_prime_UTR 45.0%
GGTGTTTATGATGGTCATGG+TGG - chr1_2:48538098-48538117 Msa0074230:three_prime_UTR 45.0%
TTGGAATGAGACGAACAGCT+TGG + chr1_2:48538385-48538404 None:intergenic 45.0%
ATCCTTCACAATCACCGCTT+TGG + chr1_2:48538518-48538537 None:intergenic 45.0%
GTACCTGCTGATGCAATATG+TGG + chr1_2:48538836-48538855 None:intergenic 45.0%
!! AGCAGGTACTTGTTGTTTGG+TGG - chr1_2:48538847-48538866 Msa0074230:intron 45.0%
!! GCAGGTACTTGTTGTTTGGT+GGG - chr1_2:48538848-48538867 Msa0074230:CDS 45.0%
!! CAGGTACTTGTTGTTTGGTG+GGG - chr1_2:48538849-48538868 Msa0074230:CDS 45.0%
AAATTCTGGTGACTCAAGGG+TGG - chr1_2:48538901-48538920 Msa0074230:CDS 45.0%
! TGGTGTTAGGAAGACTAGAG+AGG - chr1_2:48538921-48538940 Msa0074230:CDS 45.0%
GGTGTTAGGAAGACTAGAGA+GGG - chr1_2:48538922-48538941 Msa0074230:CDS 45.0%
AAGACTAGAGAGGGCAACAA+GGG - chr1_2:48538931-48538950 Msa0074230:CDS 45.0%
CACAATGTTTGGCGTGTCAA+AGG - chr1_2:48539061-48539080 Msa0074230:CDS 45.0%
GATGGCTCACAGCTAAGAAT+TGG + chr1_2:48539313-48539332 None:intergenic 45.0%
ACGAGCTGTCCGATCTTAAT+TGG - chr1_2:48539582-48539601 Msa0074230:intron 45.0%
GCTGTCCGATCTTAATTGGA+CGG - chr1_2:48539586-48539605 Msa0074230:intron 45.0%
AACTGGACAGAACAAGGTAG+GGG + chr1_2:48540294-48540313 None:intergenic 45.0%
CTGTCCAGTTTATGATCTGG+TGG - chr1_2:48540304-48540323 Msa0074230:CDS 45.0%
!!! TATTATTTATATAAATGTTT+TGG + chr1_2:48538583-48538602 None:intergenic 5.0%
!! GCAGATGGTGATGATGGAGA+TGG - chr1_2:48537894-48537913 Msa0074230:three_prime_UTR 50.0%
ATGGAGATGGGAGTGGAAGT+GGG - chr1_2:48537907-48537926 Msa0074230:three_prime_UTR 50.0%
AAGTCCCTTGAGGACCCAAA+TGG + chr1_2:48538073-48538092 None:intergenic 50.0%
TCTAAAGGCTCGTGTTTGGC+CGG - chr1_2:48538327-48538346 Msa0074230:CDS 50.0%
ATCTACACAGCCGGCTATAG+AGG + chr1_2:48538436-48538455 None:intergenic 50.0%
TATAGTCGGGTGCACCAAAG+CGG - chr1_2:48538501-48538520 Msa0074230:intron 50.0%
CACCAAAGCGGTGATTGTGA+AGG - chr1_2:48538513-48538532 Msa0074230:intron 50.0%
GGGTAGCTTAGTGCATGAGA+GGG + chr1_2:48538659-48538678 None:intergenic 50.0%
AGGGTAGCTTAGTGCATGAG+AGG + chr1_2:48538660-48538679 None:intergenic 50.0%
! TGATTTTCAGCTGCAAGCCC+TGG + chr1_2:48538725-48538744 None:intergenic 50.0%
GGGCTTGCAGCTGAAAATCA+AGG - chr1_2:48538725-48538744 Msa0074230:intron 50.0%
CAGCCACATATTGCATCAGC+AGG - chr1_2:48538830-48538849 Msa0074230:intron 50.0%
GAAGACTAGAGAGGGCAACA+AGG - chr1_2:48538930-48538949 Msa0074230:CDS 50.0%
TTGCAGGTTTCGCGATCGAT+CGG - chr1_2:48539208-48539227 Msa0074230:CDS 50.0%
GGAGCAGCTTAGCAATCAAG+AGG - chr1_2:48539396-48539415 Msa0074230:CDS 50.0%
ACTGGACAGAACAAGGTAGG+GGG + chr1_2:48540293-48540312 None:intergenic 50.0%
CCATCATCACCATCTGCAGC+TGG + chr1_2:48537891-48537910 None:intergenic 55.0%
! CCAGCTGCAGATGGTGATGA+TGG - chr1_2:48537888-48537907 Msa0074230:three_prime_UTR 55.0%
!! GGTGATGATGGAGATGGGAG+TGG - chr1_2:48537900-48537919 Msa0074230:three_prime_UTR 55.0%
GATGGAGATGGGAGTGGAAG+TGG - chr1_2:48537906-48537925 Msa0074230:three_prime_UTR 55.0%
TTCGGCCAAACATCCGTCGT+TGG - chr1_2:48538299-48538318 Msa0074230:CDS 55.0%
CAGCCGGCTATAGAGGATTC+AGG + chr1_2:48538429-48538448 None:intergenic 55.0%
GGTGACTCAAGGGTGGTGTT+AGG - chr1_2:48538908-48538927 Msa0074230:CDS 55.0%
GCGTGTCAAAGGCCTCATAC+AGG - chr1_2:48539072-48539091 Msa0074230:intron 55.0%
CAGCACTCAGAGAAGCAGCA+AGG - chr1_2:48539952-48539971 Msa0074230:intron 55.0%
TGCTGGAAGCCAGCTGCAGA+TGG - chr1_2:48537879-48537898 Msa0074230:three_prime_UTR 60.0%
GATGGGAGTGGAAGTGGGAG+TGG - chr1_2:48537912-48537931 Msa0074230:three_prime_UTR 60.0%
GGAGTGGAAGTGGGAGTGGT+GGG - chr1_2:48537916-48537935 Msa0074230:three_prime_UTR 60.0%
! GGGAGTGGTGGGAGAGTTGA+TGG - chr1_2:48537927-48537946 Msa0074230:three_prime_UTR 60.0%
GGGAGTGGAAGTGGGAGTGG+TGG - chr1_2:48537915-48537934 Msa0074230:three_prime_UTR 65.0%
AGGCTCGTGTTTGGCCGGAG+AGG - chr1_2:48538332-48538351 Msa0074230:CDS 65.0%
GTGTTTGGCCGGAGAGGCTG+CGG - chr1_2:48538338-48538357 Msa0074230:CDS 65.0%
GTGCAGCACCGCAGCCTCTC+CGG + chr1_2:48538349-48538368 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 48537867 48540431 48537867 ID=Msa0074230;Name=Msa0074230
chr1_2 mRNA 48537867 48540431 48537867 ID=Msa0074230-mRNA-1;Parent=Msa0074230;Name=Msa0074230-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0.75|1|1|1|4|286|375
chr1_2 exon 48537867 48538371 48537867 ID=Msa0074230-mRNA-1:exon:14759;Parent=Msa0074230-mRNA-1
chr1_2 exon 48538848 48539084 48538848 ID=Msa0074230-mRNA-1:exon:14758;Parent=Msa0074230-mRNA-1
chr1_2 exon 48539205 48539572 48539205 ID=Msa0074230-mRNA-1:exon:14757;Parent=Msa0074230-mRNA-1
chr1_2 exon 48540128 48540431 48540128 ID=Msa0074230-mRNA-1:exon:14756;Parent=Msa0074230-mRNA-1
chr1_2 CDS 48540128 48540431 48540128 ID=Msa0074230-mRNA-1:cds;Parent=Msa0074230-mRNA-1
chr1_2 CDS 48539205 48539572 48539205 ID=Msa0074230-mRNA-1:cds;Parent=Msa0074230-mRNA-1
chr1_2 CDS 48538848 48539084 48538848 ID=Msa0074230-mRNA-1:cds;Parent=Msa0074230-mRNA-1
chr1_2 CDS 48538153 48538371 48538153 ID=Msa0074230-mRNA-1:cds;Parent=Msa0074230-mRNA-1
chr1_2 three_prime_UTR 48537867 48538152 48537867 ID=Msa0074230-mRNA-1:three_prime_utr;Parent=Msa0074230-mRNA-1
Gene Sequence

>Msa0074230

ATGGTGAGATCTTGCTGGAAGCCAGCTGCAGATGGTGATGATGGAGATGGGAGTGGAAGTGGGAGTGGTGGGAGAGTTGATGGATTGTTATGGTACAAAGATTTAGGGAACCATATTTATGGTGAATTTTCAATGGCTGTGATTCAAGCTAATAGTTCATTGGAGGATAGAAGTGAATTTGAGTCTGGACCTTTGAGTTCTAACCATTTGGGTCCTCAAGGGACTTTTATTGGTGTTTATGATGGTCATGGTGGAGCTGAAGCTTCTCAATTTGTCAGTGACAATCTTTTCTGCAATCTCAAAAGGCTTGCAGCTGAAAATCAAGGCTTGTCGGAAAATGTGATCAAAAGGGCATTTTCAGCAACAGAAGAGAGTTTTCTATCACTTGTGAATAAACAGTGGCTAAGACAGCCACATATTGCATCAGCAGGTACTTGTTGTTTGGTGGGGATAATTTGCAATGGACTGCTATATATTGCAAATTCTGGTGACTCAAGGGTGGTGTTAGGAAGACTAGAGAGGGCAACAAGGGAAACTTCAGCCATACAATTATCTGCAGAACATAATGTTAACCTTGAAAGAGTGAGAGATGAACTTAGATTAAGACACCCTTATGATTCACAAATTGTGGTTATGAGACACAATGTTTGGCGTGTCAAAGGCCTCATACAGGTTTCGCGATCGATCGGTGATGCATATCTGAAGAAAGCAGAATTCAATAGGGAGCCTCTACCATCAAAGTTCAGACTTGCTGAAACATTTTTCAAACCAATTCTTAGCTGTGAGCCATCCATTTCATCACATAAACTTCATCCAGATGACCAATTTCTCATATTTGCTTCTGATGGGTTATGGGAGCAGCTTAGCAATCAAGAGGCTGTTAACATAGTCAACAACAATCCACCTAATGGAATTGCAAGAAGACTTGTGAAAGCAGCACTCAGAGAAGCAGCAAGGAAGAGAGAGATGAGACTAGCAGACCTTCAAAAGATTGAACAAGGAACAAGGAGACACTTCCATGATGATATAACTGTGATTGTTGTGTTTCTTAATCACAAACTGATTGATAACAGCTCTCTTTGGGGTTCTTCTCTTTCAATCAAAGGAGGTGGATCTGCCAATCACTAA
Protein sequence

>Msa0074230

MVRSCWKPAADGDDGDGSGSGSGGRVDGLLWYKDLGNHIYGEFSMAVIQANSSLEDRSEFESGPLSSNHLGPQGTFIGVYDGHGGAEASQFVSDNLFCNLKRLAAENQGLSENVIKRAFSATEESFLSLVNKQWLRQPHIASAGTCCLVGIICNGLLYIANSGDSRVVLGRLERATRETSAIQLSAEHNVNLERVRDELRLRHPYDSQIVVMRHNVWRVKGLIQVSRSIGDAYLKKAEFNREPLPSKFRLAETFFKPILSCEPSISSHKLHPDDQFLIFASDGLWEQLSNQEAVNIVNNNPPNGIARRLVKAALREAARKREMRLADLQKIEQGTRRHFHDDITVIVVFLNHKLIDNSSLWGSSLSIKGGGSANH*