AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0630960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630960 MtrunA17_Chr4g0008421 98.182 110 2 0 1 110 88 197 2.66e-74 221
Msa0630960 MtrunA17_Chr4g0008411 68.142 113 28 2 1 110 1 108 2.37e-47 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630960 AT2G37970.1 73.786 103 26 1 1 103 32 133 6.77e-46 148

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAAGATGGTGGCTTTA+TGG 0.188087 4_3:+13481806 Msa0630960:CDS
TCCATAATGGGTATGGTATT+TGG 0.221891 4_3:+13481663 None:intergenic
GCAAACTATATTGGAGCATT+AGG 0.249418 4_3:+13481834 Msa0630960:CDS
GCAGAACCCTTTGTGATAAC+TGG 0.387111 4_3:-13481900 None:intergenic
TTGTTTCCTTTGAACTGTGA+TGG 0.417876 4_3:-13481789 None:intergenic
TCACTAGCCTTAGTCACAAC+TGG 0.437262 4_3:-13481945 None:intergenic
GACAGCACCAGTTGTGACTA+AGG 0.455361 4_3:+13481938 Msa0630960:CDS
TTCTCGATCCATAATGGGTA+TGG 0.497976 4_3:+13481656 None:intergenic
GTGACTAAGGCTAGTGAAGA+AGG 0.510099 4_3:+13481951 Msa0630960:CDS
TCTGATTTCATAGTCTTGTG+TGG 0.560504 4_3:-13481727 None:intergenic
AGAAGGTGAGAAGAATAAGA+TGG 0.575015 4_3:+13481968 Msa0630960:CDS
ACTGCACCAGTTATCACAAA+GGG 0.579442 4_3:+13481894 Msa0630960:CDS
TATGATCCATCACAGTTCAA+AGG 0.581780 4_3:+13481783 Msa0630960:CDS
CAGTTCAAAGGAAACAAAGA+TGG 0.590535 4_3:+13481795 Msa0630960:CDS
GTAACTTCAGCTGCTACAGA+AGG 0.609940 4_3:-13481759 None:intergenic
TTCAAAGGAAACAAAGATGG+TGG 0.644336 4_3:+13481798 Msa0630960:CDS
GACTGCACCAGTTATCACAA+AGG 0.644397 4_3:+13481893 Msa0630960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTGTCACTTCATATTT+TGG - chr4_3:13481708-13481727 None:intergenic 25.0%
!!! ATGGTTTTAGCAAACTATAT+TGG + chr4_3:13481825-13481844 Msa0630960:CDS 25.0%
GGTATGGTATTTGGAAAAAT+TGG + chr4_3:13481672-13481691 Msa0630960:CDS 30.0%
!! TCTGATTTCATAGTCTTGTG+TGG - chr4_3:13481730-13481749 None:intergenic 35.0%
TATGATCCATCACAGTTCAA+AGG + chr4_3:13481783-13481802 Msa0630960:CDS 35.0%
TTGTTTCCTTTGAACTGTGA+TGG - chr4_3:13481792-13481811 None:intergenic 35.0%
CAGTTCAAAGGAAACAAAGA+TGG + chr4_3:13481795-13481814 Msa0630960:CDS 35.0%
TTCAAAGGAAACAAAGATGG+TGG + chr4_3:13481798-13481817 Msa0630960:CDS 35.0%
! AAACAAAGATGGTGGCTTTA+TGG + chr4_3:13481806-13481825 Msa0630960:CDS 35.0%
! GCAAACTATATTGGAGCATT+AGG + chr4_3:13481834-13481853 Msa0630960:CDS 35.0%
!!! TTTCAGGTTTTGTGTTCTGA+GGG - chr4_3:13481863-13481882 None:intergenic 35.0%
!!! TTTTCAGGTTTTGTGTTCTG+AGG - chr4_3:13481864-13481883 None:intergenic 35.0%
AGAAGGTGAGAAGAATAAGA+TGG + chr4_3:13481968-13481987 Msa0630960:CDS 35.0%
! GCAGTCATAGCAATCTTTTC+AGG - chr4_3:13481879-13481898 None:intergenic 40.0%
ACTGCACCAGTTATCACAAA+GGG + chr4_3:13481894-13481913 Msa0630960:CDS 40.0%
GTAACTTCAGCTGCTACAGA+AGG - chr4_3:13481762-13481781 None:intergenic 45.0%
GACTGCACCAGTTATCACAA+AGG + chr4_3:13481893-13481912 Msa0630960:CDS 45.0%
GCAGAACCCTTTGTGATAAC+TGG - chr4_3:13481903-13481922 None:intergenic 45.0%
!! TCACTAGCCTTAGTCACAAC+TGG - chr4_3:13481948-13481967 None:intergenic 45.0%
GTGACTAAGGCTAGTGAAGA+AGG + chr4_3:13481951-13481970 Msa0630960:CDS 45.0%
GACAGCACCAGTTGTGACTA+AGG + chr4_3:13481938-13481957 Msa0630960:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 13481669 13482001 13481669 ID=Msa0630960;Name=Msa0630960
chr4_3 mRNA 13481669 13482001 13481669 ID=Msa0630960-mRNA-1;Parent=Msa0630960;Name=Msa0630960-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|110
chr4_3 exon 13481669 13482001 13481669 ID=Msa0630960-mRNA-1:exon:3786;Parent=Msa0630960-mRNA-1
chr4_3 CDS 13481669 13482001 13481669 ID=Msa0630960-mRNA-1:cds;Parent=Msa0630960-mRNA-1
Gene Sequence

>Msa0630960

ATGGGTATGGTATTTGGAAAAATTGGTGTTGAGACACCAAAATATGAAGTGACAAAAACCACACAAGACTATGAAATCAGAATATATGCACCTTCTGTAGCAGCTGAAGTTACATATGATCCATCACAGTTCAAAGGAAACAAAGATGGTGGCTTTATGGTTTTAGCAAACTATATTGGAGCATTAGGAAACCCTCAGAACACAAAACCTGAAAAGATTGCTATGACTGCACCAGTTATCACAAAGGGTTCTGCAGAGAAGATTGCTATGACAGCACCAGTTGTGACTAAGGCTAGTGAAGAAGGTGAGAAGAATAAGATGGTGACTATGTAG

Protein sequence

>Msa0630960

MGMVFGKIGVETPKYEVTKTTQDYEIRIYAPSVAAEVTYDPSQFKGNKDGGFMVLANYIGALGNPQNTKPEKIAMTAPVITKGSAEKIAMTAPVVTKASEEGEKNKMVTM*