AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003240 MtrunA17_Chr7g0214201 95.152 165 5 1 1 165 1 162 7.00e-116 325
Msa1003240 MtrunA17_Chr3g0131991 49.612 129 54 4 7 135 1 118 1.03e-31 111
Msa1003240 MtrunA17_Chr8g0386111 41.406 128 59 3 24 144 5 123 1.65e-26 97.8
Msa1003240 MtrunA17_Chr8g0386131 53.165 79 33 1 70 144 49 127 5.07e-24 91.7
Msa1003240 MtrunA17_Chr8g0386121 53.125 64 30 0 71 134 47 110 1.05e-20 82.0
Msa1003240 MtrunA17_Chr4g0036141 53.125 64 30 0 70 133 37 100 1.01e-17 75.1
Msa1003240 MtrunA17_Chr2g0311341 43.478 92 39 1 39 130 40 118 6.85e-17 72.8
Msa1003240 MtrunA17_Chr3g0127561 42.169 83 46 1 56 136 19 101 1.18e-16 71.6
Msa1003240 MtrunA17_Chr4g0070071 45.455 66 36 0 70 135 69 134 1.20e-16 72.8
Msa1003240 MtrunA17_Chr8g0358871 50.769 65 32 0 66 130 31 95 2.19e-16 70.9
Msa1003240 MtrunA17_Chr3g0139171 35.000 140 74 4 15 145 15 146 2.44e-16 72.4
Msa1003240 MtrunA17_Chr2g0301531 40.845 71 42 0 70 140 76 146 2.67e-16 72.0
Msa1003240 MtrunA17_Chr1g0156341 41.026 78 46 0 64 141 31 108 8.04e-16 70.1
Msa1003240 MtrunA17_Chr7g0266581 43.056 72 37 1 73 144 53 120 1.09e-15 69.3
Msa1003240 MtrunA17_Chr3g0121801 40.476 84 45 2 46 128 5 84 2.46e-15 67.4
Msa1003240 MtrunA17_Chr1g0178451 42.466 73 42 0 62 134 41 113 5.02e-15 67.8
Msa1003240 MtrunA17_Chr3g0145401 38.947 95 52 3 37 129 15 105 5.41e-15 67.0
Msa1003240 MtrunA17_Chr7g0276101 44.928 69 36 1 70 136 23 91 1.55e-14 65.9
Msa1003240 MtrunA17_Chr7g0266571 45.161 62 34 0 73 134 53 114 5.19e-14 65.1
Msa1003240 MtrunA17_Chr4g0000251 35.211 71 46 0 69 139 69 139 5.82e-14 65.9
Msa1003240 MtrunA17_Chr4g0036711 41.667 72 41 1 65 135 10 81 1.14e-13 62.8
Msa1003240 MtrunA17_Chr4g0036561 38.158 76 46 1 55 129 5 80 3.15e-13 62.0
Msa1003240 MtrunA17_Chr3g0121721 38.824 85 47 2 46 129 5 85 4.04e-13 61.6
Msa1003240 MtrunA17_Chr8g0346181 43.210 81 40 3 68 142 25 105 4.97e-13 62.0
Msa1003240 MtrunA17_Chr3g0121701 36.471 85 49 2 46 129 5 85 5.00e-13 61.6
Msa1003240 MtrunA17_Chr3g0121731 36.471 85 49 2 46 129 5 85 5.00e-13 61.6
Msa1003240 MtrunA17_Chr1g0147571 39.394 66 40 0 70 135 104 169 5.48e-13 64.3
Msa1003240 MtrunA17_Chr1g0179511 37.500 64 40 0 70 133 19 82 9.36e-13 61.6
Msa1003240 MtrunA17_Chr4g0036181 41.667 72 41 1 56 126 69 140 9.97e-13 62.4
Msa1003240 MtrunA17_Chr8g0358971 45.902 61 32 1 70 129 25 85 9.99e-13 60.8
Msa1003240 MtrunA17_Chr3g0130911 38.824 85 43 2 62 138 2 85 1.12e-12 60.8
Msa1003240 MtrunA17_Chr4g0036531 40.909 66 38 1 70 134 25 90 1.26e-12 60.5
Msa1003240 MtrunA17_Chr8g0359011 48.276 58 29 1 70 126 3 60 1.30e-12 59.7
Msa1003240 MtrunA17_Chr4g0036461 47.541 61 31 1 70 129 25 85 1.42e-12 60.5
Msa1003240 MtrunA17_Chr7g0264401 45.902 61 33 0 70 130 40 100 1.50e-12 60.8
Msa1003240 MtrunA17_Chr2g0281291 49.231 65 30 2 68 129 22 86 1.67e-12 60.1
Msa1003240 MtrunA17_Chr8g0358981 36.471 85 49 2 46 129 5 85 1.70e-12 60.1
Msa1003240 MtrunA17_Chr1g0199531 44.444 63 35 0 68 130 48 110 2.02e-12 60.5
Msa1003240 MtrunA17_Chr3g0121761 47.692 65 30 3 64 126 20 82 2.06e-12 60.1
Msa1003240 MtrunA17_Chr4g0036751 36.842 76 47 1 55 129 5 80 2.60e-12 59.3
Msa1003240 MtrunA17_Chr4g0036271 40.909 66 38 1 70 134 23 88 3.99e-12 59.3
Msa1003240 MtrunA17_Chr4g0036611 45.902 61 32 1 70 129 25 85 4.06e-12 59.3
Msa1003240 MtrunA17_Chr4g0011821 38.961 77 44 1 70 143 79 155 4.87e-12 60.8
Msa1003240 MtrunA17_Chr4g0036211 34.884 86 55 1 56 140 150 235 6.87e-12 61.6
Msa1003240 MtrunA17_Chr4g0036841 46.552 58 30 1 70 126 35 92 8.72e-12 58.5
Msa1003240 MtrunA17_Chr4g0036811 39.394 66 39 1 70 134 26 91 1.06e-11 58.2
Msa1003240 MtrunA17_Chr4g0036201 36.250 80 50 1 56 134 12 91 1.13e-11 58.2
Msa1003240 MtrunA17_Chr7g0264551 38.889 72 42 1 61 130 43 114 1.21e-11 58.5
Msa1003240 MtrunA17_Chr4g0036831 37.333 75 46 1 56 129 12 86 1.41e-11 57.8
Msa1003240 MtrunA17_Chr4g0036571 35.484 93 55 3 41 129 29 120 1.93e-11 58.2
Msa1003240 MtrunA17_Chr4g0036491 47.541 61 31 1 70 129 25 85 2.15e-11 57.4
Msa1003240 MtrunA17_Chr3g0121781 45.763 59 29 2 70 126 25 82 2.51e-11 57.0
Msa1003240 MtrunA17_Chr8g0358991 45.763 59 29 2 70 126 25 82 2.65e-11 57.0
Msa1003240 MtrunA17_Chr3g0139711 35.294 85 51 2 46 129 5 86 2.68e-11 57.0
Msa1003240 MtrunA17_Chr4g0036581 47.458 59 30 1 72 129 27 85 3.83e-11 56.6
Msa1003240 MtrunA17_Chr4g0036221 42.424 66 36 2 70 134 26 90 4.22e-11 56.6
Msa1003240 MtrunA17_Chr4g0036801 44.262 61 33 1 70 129 26 86 4.27e-11 56.6
Msa1003240 MtrunA17_Chr1g0181011 31.624 117 58 2 14 130 55 149 4.29e-11 58.2
Msa1003240 MtrunA17_Chr3g0139701 44.262 61 33 1 70 129 83 143 4.57e-11 57.8
Msa1003240 MtrunA17_Chr3g0121741 44.068 59 30 2 70 126 25 82 6.57e-11 55.8
Msa1003240 MtrunA17_Chr4g0036431 45.763 59 31 1 72 129 27 85 7.95e-11 55.8
Msa1003240 MtrunA17_Chr4g0036191 46.667 60 29 2 71 129 27 84 8.18e-11 55.8
Msa1003240 MtrunA17_Chr3g0121711 44.068 59 30 2 70 126 25 82 9.12e-11 55.5
Msa1003240 MtrunA17_Chr4g0036651 40.625 64 37 1 72 134 27 90 9.92e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003240 AT5G50760.1 44.828 116 61 2 19 133 5 118 2.11e-23 91.3
Msa1003240 AT1G56150.1 47.887 71 37 0 60 130 28 98 4.81e-18 75.5
Msa1003240 AT5G20810.1 46.970 66 35 0 70 135 79 144 2.83e-17 74.7
Msa1003240 AT5G20810.2 46.970 66 35 0 70 135 79 144 4.13e-17 75.1
Msa1003240 AT3G43120.1 45.455 66 36 0 70 135 79 144 8.25e-17 73.6
Msa1003240 AT3G12830.1 44.048 84 44 2 49 130 28 110 3.47e-16 71.2
Msa1003240 AT2G46690.1 45.833 72 36 1 68 136 22 93 1.39e-15 69.3
Msa1003240 AT3G61900.1 41.379 87 39 2 68 142 28 114 1.76e-15 69.3
Msa1003240 AT1G16510.1 46.970 66 35 0 65 130 49 114 4.27e-15 68.9
Msa1003240 AT4G00880.1 40.964 83 45 2 56 136 16 96 1.13e-14 67.0
Msa1003240 AT4G34750.2 44.262 61 34 0 70 130 37 97 1.27e-14 67.4
Msa1003240 AT4G34750.1 44.262 61 34 0 70 130 37 97 1.27e-14 67.4
Msa1003240 AT1G75590.1 49.123 57 29 0 70 126 42 98 7.23e-14 65.9
Msa1003240 AT1G79130.1 36.111 108 56 2 26 133 17 111 1.34e-13 64.3
Msa1003240 AT5G10990.1 49.123 57 29 0 70 126 41 97 1.38e-13 64.7
Msa1003240 AT5G53590.1 42.466 73 37 1 70 137 46 118 1.72e-13 64.3
Msa1003240 AT1G19840.1 44.262 61 34 0 70 130 41 101 1.43e-12 62.4
Msa1003240 AT2G24400.1 40.230 87 50 1 52 136 46 132 2.03e-12 62.4
Msa1003240 AT4G31320.1 40.000 80 43 2 71 145 81 160 5.60e-12 61.2
Msa1003240 AT3G20220.1 38.235 68 41 1 65 131 42 109 9.63e-12 59.3
Msa1003240 AT2G37030.1 40.984 61 36 0 70 130 49 109 2.83e-11 58.2
Msa1003240 AT3G60690.1 38.542 96 52 3 58 148 74 167 3.94e-11 58.9
Msa1003240 AT3G51200.1 36.923 65 41 0 66 130 36 100 6.32e-11 56.6
Msa1003240 AT5G66260.1 36.364 66 41 1 70 135 34 98 6.57e-11 56.6
Msa1003240 AT2G36210.1 31.707 123 67 4 14 130 4 115 8.57e-11 57.0

Find 42 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCTTCTTAGCCCTTCC+AGG 0.273002 7_1:-291415 Msa1003240:CDS
CCTTCCCAAGGTTGTACTTT+TGG 0.273068 7_1:-291455 Msa1003240:CDS
ACCACAAACCTTTGCATTTG+AGG 0.340829 7_1:+291626 None:intergenic
GGAAAAGGGAACAAGAAAAT+AGG 0.347522 7_1:-291839 Msa1003240:CDS
GAAGAAGCTGAATCAGAATA+TGG 0.364921 7_1:-291560 Msa1003240:CDS
TTACAAAGTGTTGATGGAAA+TGG 0.371199 7_1:-291489 Msa1003240:CDS
TTATAGCTGCCAAGGACCTT+TGG 0.404368 7_1:-291537 Msa1003240:CDS
AAGAACATGAAGCAAGGAAA+AGG 0.428717 7_1:-291854 Msa1003240:CDS
ACCTCAAATGCAAAGGTTTG+TGG 0.451149 7_1:-291627 Msa1003240:CDS
GTTGTTTAAGACAATCATCC+TGG 0.454314 7_1:+291397 None:intergenic
GATTTGCTCCTTCTTGTCGT+TGG 0.461730 7_1:+291728 None:intergenic
TAAAAGACATCAACATTACA+AGG 0.510903 7_1:+291509 None:intergenic
AGAACATGAAGCAAGGAAAA+GGG 0.512113 7_1:-291853 Msa1003240:CDS
TGCAAGTCCATAGGAGGAGG+AGG 0.512745 7_1:-291791 Msa1003240:CDS
ATCATGGCCTAGTCTTCCGC+GGG 0.516084 7_1:-291708 Msa1003240:CDS
AAGGAAGAGCCAAAGGTCCT+TGG 0.517712 7_1:+291528 None:intergenic
CGACAAGAAGGAGCAAATCA+TGG 0.519386 7_1:-291724 Msa1003240:CDS
TAGACAAAAGAAAGTGGCCC+CGG 0.522285 7_1:-291675 Msa1003240:CDS
ACACCGAAAAGCATCCCTCC+GGG 0.524201 7_1:+291657 None:intergenic
TCAAGCAACATCTTGAACAA+AGG 0.532123 7_1:+291581 None:intergenic
ACGACCAAAAGTACAACCTT+GGG 0.533998 7_1:+291451 None:intergenic
AACGACCAAAAGTACAACCT+TGG 0.538821 7_1:+291450 None:intergenic
AGATGCAAGTCCATAGGAGG+AGG 0.541895 7_1:-291794 Msa1003240:CDS
TACACCGAAAAGCATCCCTC+CGG 0.548704 7_1:+291656 None:intergenic
AAGTCCATAGGAGGAGGAGG+AGG 0.549753 7_1:-291788 Msa1003240:CDS
AGAAAATAGACAAAAGAAAG+TGG 0.550670 7_1:-291681 Msa1003240:CDS
GAATATGGTTATAGCTGCCA+AGG 0.552741 7_1:-291545 Msa1003240:CDS
CAAAAGAAAGTGGCCCCGGA+GGG 0.557663 7_1:-291671 Msa1003240:CDS
CAGTGATTCCAACGACAAGA+AGG 0.563887 7_1:-291736 Msa1003240:CDS
TTTAAGACAATCATCCTGGA+AGG 0.580942 7_1:+291401 None:intergenic
TGGGAGAGATGCAAGTCCAT+AGG 0.597578 7_1:-291800 Msa1003240:CDS
AATCATGGCCTAGTCTTCCG+CGG 0.600149 7_1:-291709 Msa1003240:CDS
GAGAGATGCAAGTCCATAGG+AGG 0.601664 7_1:-291797 Msa1003240:CDS
TTAAGACAATCATCCTGGAA+GGG 0.611880 7_1:+291402 None:intergenic
ACATTACAAGGAAGAGCCAA+AGG 0.626356 7_1:+291521 None:intergenic
ACGTTGGACCTCAAATGCAA+AGG 0.634178 7_1:-291634 Msa1003240:CDS
ATTGGCAAGAACATGAAGCA+AGG 0.634830 7_1:-291860 Msa1003240:CDS
GATAACGAAGCTCCTTCCCA+AGG 0.635029 7_1:-291467 Msa1003240:CDS
ACAAAAGAAAGTGGCCCCGG+AGG 0.644682 7_1:-291672 Msa1003240:CDS
CCAAAAGTACAACCTTGGGA+AGG 0.674764 7_1:+291455 None:intergenic
CACCGAAAAGCATCCCTCCG+GGG 0.706013 7_1:+291658 None:intergenic
TCATGGCCTAGTCTTCCGCG+GGG 0.775767 7_1:-291707 Msa1003240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAATAGACAAAAGAAAG+TGG - chr7_1:291576-291595 Msa1003240:CDS 25.0%
! TAAAAGACATCAACATTACA+AGG + chr7_1:291751-291770 None:intergenic 25.0%
!!! TGTCTTTTACAAAGTGTTGA+TGG - chr7_1:291762-291781 Msa1003240:CDS 30.0%
!! TTACAAAGTGTTGATGGAAA+TGG - chr7_1:291768-291787 Msa1003240:CDS 30.0%
AAGAACATGAAGCAAGGAAA+AGG - chr7_1:291403-291422 Msa1003240:CDS 35.0%
AGAACATGAAGCAAGGAAAA+GGG - chr7_1:291404-291423 Msa1003240:CDS 35.0%
GGAAAAGGGAACAAGAAAAT+AGG - chr7_1:291418-291437 Msa1003240:CDS 35.0%
TCAAGCAACATCTTGAACAA+AGG + chr7_1:291679-291698 None:intergenic 35.0%
GAAGAAGCTGAATCAGAATA+TGG - chr7_1:291697-291716 Msa1003240:CDS 35.0%
TTAAGACAATCATCCTGGAA+GGG + chr7_1:291858-291877 None:intergenic 35.0%
! TTTAAGACAATCATCCTGGA+AGG + chr7_1:291859-291878 None:intergenic 35.0%
! GTTGTTTAAGACAATCATCC+TGG + chr7_1:291863-291882 None:intergenic 35.0%
ATTGGCAAGAACATGAAGCA+AGG - chr7_1:291397-291416 Msa1003240:CDS 40.0%
TAGGCCTCATAACAAAAACG+TGG - chr7_1:291437-291456 Msa1003240:CDS 40.0%
AGGCCTCATAACAAAAACGT+GGG - chr7_1:291438-291457 Msa1003240:CDS 40.0%
!! TCTCCCACGTTTTTGTTATG+AGG + chr7_1:291444-291463 None:intergenic 40.0%
ACCTCAAATGCAAAGGTTTG+TGG - chr7_1:291630-291649 Msa1003240:CDS 40.0%
ACCACAAACCTTTGCATTTG+AGG + chr7_1:291634-291653 None:intergenic 40.0%
GAATATGGTTATAGCTGCCA+AGG - chr7_1:291712-291731 Msa1003240:CDS 40.0%
ACATTACAAGGAAGAGCCAA+AGG + chr7_1:291739-291758 None:intergenic 40.0%
! ACGACCAAAAGTACAACCTT+GGG + chr7_1:291809-291828 None:intergenic 40.0%
! AACGACCAAAAGTACAACCT+TGG + chr7_1:291810-291829 None:intergenic 40.0%
CAGTGATTCCAACGACAAGA+AGG - chr7_1:291521-291540 Msa1003240:CDS 45.0%
! GATTTGCTCCTTCTTGTCGT+TGG + chr7_1:291532-291551 None:intergenic 45.0%
CGACAAGAAGGAGCAAATCA+TGG - chr7_1:291533-291552 Msa1003240:CDS 45.0%
TAGACAAAAGAAAGTGGCCC+CGG - chr7_1:291582-291601 Msa1003240:CDS 45.0%
ACGTTGGACCTCAAATGCAA+AGG - chr7_1:291623-291642 Msa1003240:CDS 45.0%
TTATAGCTGCCAAGGACCTT+TGG - chr7_1:291720-291739 Msa1003240:CDS 45.0%
! CCAAAAGTACAACCTTGGGA+AGG + chr7_1:291805-291824 None:intergenic 45.0%
! CCTTCCCAAGGTTGTACTTT+TGG - chr7_1:291802-291821 Msa1003240:CDS 45.0%
ATCATCTTCTTAGCCCTTCC+AGG - chr7_1:291842-291861 Msa1003240:CDS 45.0%
TGGGAGAGATGCAAGTCCAT+AGG - chr7_1:291457-291476 Msa1003240:CDS 50.0%
GAGAGATGCAAGTCCATAGG+AGG - chr7_1:291460-291479 Msa1003240:CDS 50.0%
AGATGCAAGTCCATAGGAGG+AGG - chr7_1:291463-291482 Msa1003240:CDS 50.0%
! AATCATGGCCTAGTCTTCCG+CGG - chr7_1:291548-291567 Msa1003240:CDS 50.0%
! TGTCTATTTTCTTCGCCCCG+CGG + chr7_1:291568-291587 None:intergenic 50.0%
!! TACACCGAAAAGCATCCCTC+CGG + chr7_1:291604-291623 None:intergenic 50.0%
!! GGATGCTTTTCGGTGTACGT+TGG - chr7_1:291607-291626 Msa1003240:CDS 50.0%
AAGGAAGAGCCAAAGGTCCT+TGG + chr7_1:291732-291751 None:intergenic 50.0%
GATAACGAAGCTCCTTCCCA+AGG - chr7_1:291790-291809 Msa1003240:CDS 50.0%
TGCAAGTCCATAGGAGGAGG+AGG - chr7_1:291466-291485 Msa1003240:CDS 55.0%
AAGTCCATAGGAGGAGGAGG+AGG - chr7_1:291469-291488 Msa1003240:CDS 55.0%
! ATCATGGCCTAGTCTTCCGC+GGG - chr7_1:291549-291568 Msa1003240:CDS 55.0%
ACAAAAGAAAGTGGCCCCGG+AGG - chr7_1:291585-291604 Msa1003240:CDS 55.0%
CAAAAGAAAGTGGCCCCGGA+GGG - chr7_1:291586-291605 Msa1003240:CDS 55.0%
!! ACACCGAAAAGCATCCCTCC+GGG + chr7_1:291603-291622 None:intergenic 55.0%
TCCATAGGAGGAGGAGGAGG+AGG - chr7_1:291472-291491 Msa1003240:CDS 60.0%
TCCTCCTCCTCCTCCTCCTA+TGG + chr7_1:291476-291495 None:intergenic 60.0%
! TCATGGCCTAGTCTTCCGCG+GGG - chr7_1:291550-291569 Msa1003240:CDS 60.0%
!! CACCGAAAAGCATCCCTCCG+GGG + chr7_1:291602-291621 None:intergenic 60.0%
TCTTCGCCCCGCGGAAGACT+AGG + chr7_1:291559-291578 None:intergenic 65.0%
! GGCCCCGGAGGGATGCTTTT+CGG - chr7_1:291597-291616 Msa1003240:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 291391 291888 291391 ID=Msa1003240;Name=Msa1003240
chr7_1 mRNA 291391 291888 291391 ID=Msa1003240-mRNA-1;Parent=Msa1003240;Name=Msa1003240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|165
chr7_1 exon 291391 291888 291391 ID=Msa1003240-mRNA-1:exon:70;Parent=Msa1003240-mRNA-1
chr7_1 CDS 291391 291888 291391 ID=Msa1003240-mRNA-1:cds;Parent=Msa1003240-mRNA-1
Gene Sequence

>Msa1003240

ATGGATATTGGCAAGAACATGAAGCAAGGAAAAGGGAACAAGAAAATAGGCCTCATAACAAAAACGTGGGAGAGATGCAAGTCCATAGGAGGAGGAGGAGGAGGACACAAGAGTAAATCATATTCATCATCAGTGATTCCAACGACAAGAAGGAGCAAATCATGGCCTAGTCTTCCGCGGGGCGAAGAAAATAGACAAAAGAAAGTGGCCCCGGAGGGATGCTTTTCGGTGTACGTTGGACCTCAAATGCAAAGGTTTGTGGTCAAAACTGAGTATGCTAATCATCCTTTGTTCAAGATGTTGCTTGAAGAAGCTGAATCAGAATATGGTTATAGCTGCCAAGGACCTTTGGCTCTTCCTTGTAATGTTGATGTCTTTTACAAAGTGTTGATGGAAATGGATAACGAAGCTCCTTCCCAAGGTTGTACTTTTGGTCGTTCTCGTTCTTCTTATCATCTTCTTAGCCCTTCCAGGATGATTGTCTTAAACAACTTCTAA

Protein sequence

>Msa1003240

MDIGKNMKQGKGNKKIGLITKTWERCKSIGGGGGGHKSKSYSSSVIPTTRRSKSWPSLPRGEENRQKKVAPEGCFSVYVGPQMQRFVVKTEYANHPLFKMLLEEAESEYGYSCQGPLALPCNVDVFYKVLMEMDNEAPSQGCTFGRSRSSYHLLSPSRMIVLNNF*